The International journal of developmental biology最新文献

Follistatin regulates tooth root furcation morphogenesis. 卵泡素调节牙根分叉的形态发生。

IF 1.3

The International journal of developmental biology Pub Date : 2026-09-16 DOI: 10.1387/ijdb.28998

Liu Yang, Ju-Kyung Jeong, Hwajung Choi, Tak-Heun Kim, Hyuk-Jae Edward Kwon, Sung-Won Cho, Martin Matthew Matzuk, Eui-Sic Cho

{"title":"Follistatin regulates tooth root furcation morphogenesis.","authors":"Liu Yang, Ju-Kyung Jeong, Hwajung Choi, Tak-Heun Kim, Hyuk-Jae Edward Kwon, Sung-Won Cho, Martin Matthew Matzuk, Eui-Sic Cho","doi":"10.1387/ijdb.28998","DOIUrl":"10.1387/ijdb.28998","url":null,"abstract":"Follistatin (Fst), an extracellular inhibitor of the TGF-β signaling pathway, plays a critical role in tooth development, particularly in crown morphogenesis and enamel patterning. While molar root number is conserved within species and between upper and lower jaws, the molecular mechanisms governing root patterning remain largely unknown. To investigate the role of Fst in root formation, we analyzed K14-Cre-mediated Fst knockout mice. During embryonic and early postnatal stages, Fst was dynamically expressed in the dental epithelium including the lingual epithelium, and primary and secondary enamel knots, as well as in the dental mesenchyme at the base of the dental papilla. However, during root formation, Fst expression became restricted to the outer layer of Hertwig's epithelial root sheath (HERS). Micro-CT analysis revealed that mutant mice exhibited molar root abnormalities, including enlarged pulp chambers and partial furcation defects on the lingual side of the mandibular molars. Histological analysis showed that, while HERS initially exhibited buccolingual asymmetry - with the buccal-side HERS extending more prominently in both control and mutant mice - control mice eventually established bucco-lingual symmetry in HERS extension, ensuring proper root furcation formation. In contrast, mutant mice displayed delayed lingual-side HERS extension, resulting in defective root furcation. Molecular analysis revealed that Gli1 expression was upregulated, whereas Cdkn1a and Wnt signaling molecules (Wnt4, Lhx6, and Notum) were significantly downregulated in the delayed lingual HERS of mutant mice. These findings suggest that Fst regulates tooth root morphogenesis by modulating Shh and Wnt signaling to control lingual epithelial proliferation and HERS extension. This study offers novel insights into the molecular mechanisms governing molar root number and morphology determination.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"43-54"},"PeriodicalIF":1.3,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149121366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schlafen regulates gastrulation in Xenopus laevis early embryonic development and exhibits immune tissue-specific expression in adult frogs. Schlafen调节非洲爪蟾早期胚胎发育的原肠胚形成，并在成年蛙中表现出免疫组织特异性表达。

IF 1.3

The International journal of developmental biology Pub Date : 2026-09-16 DOI: 10.1387/ijdb.28997

Gianmatteo Vit, Alexander Hirth, Bianca N Kraft, Anna Cazzola, Harald Klüter, Christof Niehrs, Alwin Krämer

{"title":"Schlafen regulates gastrulation in Xenopus laevis early embryonic development and exhibits immune tissue-specific expression in adult frogs.","authors":"Gianmatteo Vit, Alexander Hirth, Bianca N Kraft, Anna Cazzola, Harald Klüter, Christof Niehrs, Alwin Krämer","doi":"10.1387/ijdb.28997","DOIUrl":"10.1387/ijdb.28997","url":null,"abstract":"Schlafen genes are conserved regulators of cell proliferation and play a pivotal role in immunity and host defence, yet their function during vertebrate development remains poorly understood. Here, we characterize the expression, phosphorylation dynamics, and function of the single Schlafen ortholog in Xenopus laevis (slfn13) during embryogenesis and in adult tissues. Slfn13 protein levels increase markedly around the mid-blastula transition/late blastula, with unphosphorylated Slfn13 predominating during gastrulation and neurulation, while phosphorylated Slfn13 (p-Slfn13) is more abundant during early cleavage stages. We found that Morpholino-mediated knockdown of Slfn13 in Xenopus laevis causes delayed blastopore lip formation, impaired blastopore closure, and severe gastrulation defects in a dose-dependent manner, indicating a non-redundant requirement for Slfn13 in early morphogenesis. We further analysed Slfn13 expression in adult Xenopus laevis. Slfn13 is enriched in immune tissues and thymic tumour cell lines, with p-Slfn13 specifically detected in proliferative tumour-derived cells. Collectively, our findings reveal a previously unrecognized role for slfn13 in gastrulation and support a conserved immuneassociated expression pattern in adult frogs, expanding the functional landscape of Schlafen proteins across vertebrates.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"31-42"},"PeriodicalIF":1.3,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149121360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regeneration can take place across Drosophila compartments or segments with different Hox gene expression. 再生可以发生在具有不同Hox基因表达的果蝇隔室或区段之间。

IF 1.3

The International journal of developmental biology Pub Date : 2026-09-16 DOI: 10.1387/ijdb.28996

Rafael Alejandro Juárez-Uribe, Paloma Martín, Laura Utiel, Blanca L Arrabal, Marina Blanco, Roberto Yagüe-Serrano, Eduardo Cazalla, Ernesto Sánchez-Herrero

引用次数: 0

Forkhead as a cross-regulator of cell death and the innate immune response in the Malpighian tubules of Drosophila melanogaster. 叉头蛋白作为黑腹果蝇马氏小管细胞死亡和先天免疫反应的交叉调节因子。

IF 1.3

The International journal of developmental biology Pub Date : 2026-09-15 DOI: 10.1387/ijdb.28995

Shainy Ojha, Priyanka Jaiswal, Madhu G Tapadia

{"title":"Forkhead as a cross-regulator of cell death and the innate immune response in the Malpighian tubules of Drosophila melanogaster.","authors":"Shainy Ojha, Priyanka Jaiswal, Madhu G Tapadia","doi":"10.1387/ijdb.28995","DOIUrl":"10.1387/ijdb.28995","url":null,"abstract":"Forkhead (fkh) transcription factor derives its name from the distinctive appearance of the DNA-binding motifs when attached to DNA. In mammals, they are grouped into seven families from FOXA to FOXS. FoxA subfamily participates in several essential functions, including the development of various organs, the regulation of senescence, proliferation, and metabolic homeostasis. In this study, we provide evidence that the Drosophila orthologue of FOXA, known as fkh, is instrumental in preventing programmed cell death in Drosophila Malpighian tubules (MTs) during metamorphosis. This characteristic is unique to Malpighian tubules when compared to several other larval tissues. We also demonstrate its role in the immune function of the MTs, as its depletion in MTs suppresses the expression of Anti-Microbial Peptides (AMPs). Furthermore, reduced expression of NF-kB transcription factor, Relish and Dorsal, could contribute to the reduced synthesis of AMPs. Fkh also regulates the fluid secretion of the MTs and its absence disrupts the ion transporters thus affecting fluid homeostasis. Our results demonstrate that fkh plays an essential role in the survival of MTs, without that the MTs cannot survive and fail to mount an effective immune response.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"1-13"},"PeriodicalIF":1.3,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149077259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal expression of carnitine palmitoyltransferase I genes during zebrafish development and heart regeneration. 斑马鱼发育和心脏再生过程中肉毒碱棕榈酰转移酶I基因的时空表达。

IF 1.3

The International journal of developmental biology Pub Date : 2025-11-19 DOI: 10.1387/ijdb.250046yh

Wenping Huang, Chang Kong, Xiaohan Cheng, Zongyi Duan, Hao Cao, Yanchao Han

{"title":"Spatiotemporal expression of carnitine palmitoyltransferase I genes during zebrafish development and heart regeneration.","authors":"Wenping Huang, Chang Kong, Xiaohan Cheng, Zongyi Duan, Hao Cao, Yanchao Han","doi":"10.1387/ijdb.250046yh","DOIUrl":"10.1387/ijdb.250046yh","url":null,"abstract":"Carnitine palmitoyltransferase 1 (CPT1) is a key regulatory enzyme in fatty acid metabolism, responsible for the translocation of long-chain fatty acids into the mitochondria for β-oxidation in diverse biological contexts. Recent studies implicated the critical role of cpt1 genes during zebrafish development and heart regeneration; however, a comprehensive characterization of their spatiotemporal expression dynamics remains lacking. Here, we systematically analyzed the expression profiles of four cpt1 paralogs (cpt1aa, cpt1ab, cpt1b, and cpt1a2b) during zebrafish embryogenesis and the expression of cpt1ab and cpt1b during zebrafish heart regeneration. Our results reveal that these paralogs exhibit distinct spatiotemporal expression patterns during zygotic development. While cpt1aa and cpt1ab share high sequence conservation (77%), their expression patterns diverge substantially. Conversely, cpt1ab and cpt1b display convergent cardiac and somitic expression despite lower sequence similarity (53%). Following ventricular ablation, cpt1b expression transiently ceased then recovered during regeneration, whereas cpt1ab remained unchanged. These findings shed light on the evolutionary conservation and functional divergence of cpt1 paralogs, which establish a critical foundation for elucidating paralog-specific roles in fatty acid metabolism during vertebrate development and regeneration.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 3","pages":"143-150"},"PeriodicalIF":1.3,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osteogenic gene expression in the temporal region of the opossum embryos: an insight into the evolution of synapsid skull unique to mammalian lineage. 负鼠胚胎颞区成骨基因表达：对哺乳动物谱系特有的突触颅骨进化的洞察。

IF 1.3

The International journal of developmental biology Pub Date : 2025-11-19 DOI: 10.1387/ijdb.250093mt

Shunsuke Mizuno, Hiromu Sato, Riko Yoshimi, Hiroshi Kiyonari, Masayoshi Tokita

{"title":"Osteogenic gene expression in the temporal region of the opossum embryos: an insight into the evolution of synapsid skull unique to mammalian lineage.","authors":"Shunsuke Mizuno, Hiromu Sato, Riko Yoshimi, Hiroshi Kiyonari, Masayoshi Tokita","doi":"10.1387/ijdb.250093mt","DOIUrl":"10.1387/ijdb.250093mt","url":null,"abstract":"The skull of amniotes is categorized into three conditions based on skeletal arrangement in the temporal region: anapsid, synapsid and diapsid. Mammals (class Mammalia), a descendent lineage of the clade Synapsida, possess the synapsid skull, which is characterized by a single lower temporal arch that ventrally borders the lower temporal fenestra. Although we previously suggested, based on the data from placental mammals, that the reduction in the expression domain of the upstream osteogenic genes Msx2 and Runx2 in the embryonic temporal mesenchyme might have played a role in the evolution of synapsid skulls, the molecular basis of synapsid skull evolution is still largely unknown. In this study, we investigated expression patterns of four osteogenic genes (two upstream genes Msx2 and Runx2 and two downstream genes Sp7 and Sparc) in the embryonic and neonatal temporal region of the gray short-tailed opossum Monodelphis domestica, the most commonly used experimental marsupial model, in order to more thoroughly understand the molecular basis of development of synapsid skulls unique to mammals. We found that M. domestica embryos and neonates display very restricted expressions of Msx2 and/or Runx2 in the dermal bone precursors in the temporal region, as two placental species do (the house mouse Mus musculus and the greater horseshoe bat Rhinolophus ferrumequinum). Spatially restricted expression of Msx2 and Runx2 in the embryonic temporal region may be a foundation for creating the \"advanced\" synapsid skull shared by all mammals where only three dermal bones configure the temporal region.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 3","pages":"151-158"},"PeriodicalIF":1.3,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Inhibition of COX2 impairs angiogenesis and causes vascular defects in developing zebrafish embryos. 更正：抑制COX2会损害血管生成并导致斑马鱼胚胎发育中的血管缺陷。

IF 1.3

The International journal of developmental biology Pub Date : 2025-11-19 DOI: 10.1387/ijdb.250154sb

Lakshmi Pillai, Vishakha Nesari, Dhanush Danes, Suresh Balakrishnan

引用次数: 0

Comprehensive analysis of Ephrin ligand and receptor expression reveals exclusive domains during nephrogenesis for epha4/epha7 and efna3. 综合分析Ephrin配体和受体的表达，揭示了epha4/epha7和efna3在肾形成过程中的专属结构域。

IF 1.3

The International journal of developmental biology Pub Date : 2025-10-13 DOI: 10.1387/ijdb.250065jr

Ronan Le Bouffant, Valérie Bello, Isabelle Buisson, Muriel Umbhauer, Jean-François Riou

{"title":"Comprehensive analysis of Ephrin ligand and receptor expression reveals exclusive domains during nephrogenesis for epha4/epha7 and efna3.","authors":"Ronan Le Bouffant, Valérie Bello, Isabelle Buisson, Muriel Umbhauer, Jean-François Riou","doi":"10.1387/ijdb.250065jr","DOIUrl":"10.1387/ijdb.250065jr","url":null,"abstract":"Interactions between ephrins and their Eph receptors regulate a broad range of cellular processes, including attraction, repulsion, adhesion and migration, all of which play crucial roles in tissue remodeling and homeostasis. While several ephrin ligands and Eph receptors are known to be expressed in the developing kidney, their specific roles, particularly during nephrogenesis, remain poorly understood. The development of the Xenopus pronephros provides an accessible and relatively simple model for studying vertebrate nephrogenesis. Through a comprehensive gene expression analysis of all ephrin ligands and Eph receptors present in Xenopus genomes, we have identified members of the Eph-ephrin signaling pathway that may contribute to pronephric development. Among them, efna3, which encodes an ephrin ligand, is strongly expressed in the ventral region of the pronephric anlage and later in the intermediate and distal segments of the developing tubule. This expression pattern is strikingly complementary to that of epha4 and epha7, which are expressed in the region forming the proximal tubule. This suggests a potential role for efna3-epha4/epha7 signaling in establishing a boundary between these domains during pronephros development.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 3","pages":"133-142"},"PeriodicalIF":1.3,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145644093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of the dorsal thalamus in a reptile: identification of subdivisions and their associated nuclei. 爬行动物背侧丘脑的发育：分支及其相关核的鉴定。

IF 1.3

The International journal of developmental biology Pub Date : 2025-09-03 DOI: 10.1387/ijdb.250018mp

Michael B Pritz

{"title":"Development of the dorsal thalamus in a reptile: identification of subdivisions and their associated nuclei.","authors":"Michael B Pritz","doi":"10.1387/ijdb.250018mp","DOIUrl":"10.1387/ijdb.250018mp","url":null,"abstract":"How the dorsal thalamus of amniotes (reptiles, birds, and mammals) is organized remains an important but incompletely answered question. Identification of meaningful subdivisions would greatly aid in its understanding. Because the dorsal thalamus is more simply organized during development, studies have examined this structure during embryogenesis. Most reports using this approach have examined the developing dorsal thalamus in mammals and birds. Only rarely has the development of the dorsal thalamus been investigated in reptiles. Regardless, any approach to identify subdivisions, the presumed building blocks of the dorsal thalamus, should include representatives of all three classes of vertebrates. To fill this gap in knowledge, the development of the dorsal thalamus was investigated in Alligator mississippiensis, a member of the reptilian group most closely related to birds. As the first detailed study of its kind, cytoarchitecture and calretinin expression were used to examine dorsal thalamus development. Three subdivisions, termed tiers, and the individual nuclei originating from each tier, were identified. These three tiers were similar to the subdivisions found in birds and, to a limited extent, in mammals. Taken together, these early subdivisions may represent the common building blocks of the dorsal thalamus and provide clues to understand how evolution has sculpted this structure in amniotes.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"109-122"},"PeriodicalIF":1.3,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innervation of the masseter requires Mllt11 (Af1q/Tcf7c) function during trigeminal ganglion development. 在三叉神经节发育过程中，咬肌的神经支配需要Mllt11 （Af1q/Tcf7c）的功能。

IF 1.3

The International journal of developmental biology Pub Date : 2025-08-01 DOI: 10.1387/ijdb.240249ai

Nicholas W Zinck, Danielle Stanton-Turcotte, Emily A Witt, Marley Blommers, Angelo Iulianella

{"title":"Innervation of the masseter requires Mllt11 (Af1q/Tcf7c) function during trigeminal ganglion development.","authors":"Nicholas W Zinck, Danielle Stanton-Turcotte, Emily A Witt, Marley Blommers, Angelo Iulianella","doi":"10.1387/ijdb.240249ai","DOIUrl":"10.1387/ijdb.240249ai","url":null,"abstract":"The development of cranial nerves, including the trigeminal nerve, and the formation of neuromuscular junctions (NMJs) are crucial processes for craniofacial motor function. Mllt11/Af1q/Tcf7c (hereafter Mllt11), a novel type of cytoskeletal-interacting protein, has been implicated in neuronal migration and neuritogenesis during central nervous system development. However, its role in peripheral nerve development and NMJ formation remains poorly understood. This study investigates the function of Mllt11 during trigeminal ganglion development and its impact on motor innervation of the masseter muscle. We report Mllt11 expression in the developing trigeminal ganglia, suggesting a potential role in cranial nerve development. Using a conditional knockout mouse model to delete Mllt11 in Wnt1-expressing neural crest cells, we assessed trigeminal ganglion development and innervation of the masseter muscle in the jaw. Surprisingly, we found that Mllt11 loss does not affect the initial formation of the trigeminal ganglion but disrupts its placodal vs. neural crest cellular composition. Furthermore, we showed that conditional inactivation of Mllt11 using Wnt1Cre2 led to a reduction of neurofilament density and NMJs within the masseter muscle, along with a reduction of Phox2b+ branchiomotor neurons in rhombomere 2, indicating altered trigeminal motor innervation. This was due to the surprising finding that the Wnt1Cre2/+ mouse driver promoted aberrant recombination and reporter gene expression within branchiomotor neuron pools in rhombomere 2, as well as targeting neural crest cell populations. Our findings show that Mllt11 regulates the cellular composition of the trigeminal ganglion and is essential for proper trigeminal motor innervation in the masseter muscle.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"123-131"},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0