The International journal of developmental biology最新文献

Development of the dorsal thalamus in a reptile: identification of subdivisions and their associated nuclei. 爬行动物背侧丘脑的发育：分支及其相关核的鉴定。

IF 1.3

The International journal of developmental biology Pub Date : 2025-09-03 DOI: 10.1387/ijdb.250018mp

Michael B Pritz

{"title":"Development of the dorsal thalamus in a reptile: identification of subdivisions and their associated nuclei.","authors":"Michael B Pritz","doi":"10.1387/ijdb.250018mp","DOIUrl":"https://doi.org/10.1387/ijdb.250018mp","url":null,"abstract":"How the dorsal thalamus of amniotes (reptiles, birds, and mammals) is organized remains an important but incompletely answered question. Identification of meaningful subdivisions would greatly aid in its understanding. Because the dorsal thalamus is more simply organized during development, studies have examined this structure during embryogenesis. Most reports using this approach have examined the developing dorsal thalamus in mammals and birds. Only rarely has the development of the dorsal thalamus been investigated in reptiles. Regardless, any approach to identify subdivisions, the presumed building blocks of the dorsal thalamus, should include representatives of all three classes of vertebrates. To fill this gap in knowledge, the development of the dorsal thalamus was investigated in Alligator mississippiensis, a member of the reptilian group most closely related to birds. As the first detailed study of its kind, cytoarchitecture and calretinin expression were used to examine dorsal thalamus development. Three subdivisions, termed tiers, and the individual nuclei originating from each tier, were identified. These three tiers were similar to the subdivisions found in birds and, to a limited extent, in mammals. Taken together, these early subdivisions may represent the common building blocks of the dorsal thalamus and provide clues to understand how evolution has sculpted this structure in amniotes.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innervation of the masseter requires Mllt11 (Af1q/Tcf7c) function during trigeminal ganglion development. 在三叉神经节发育过程中，咬肌的神经支配需要Mllt11 （Af1q/Tcf7c）的功能。

IF 1.3

The International journal of developmental biology Pub Date : 2025-08-01 DOI: 10.1387/ijdb.240249ai

Nicholas W Zinck, Danielle Stanton-Turcotte, Emily A Witt, Marley Blommers, Angelo Iulianella

{"title":"Innervation of the masseter requires Mllt11 (Af1q/Tcf7c) function during trigeminal ganglion development.","authors":"Nicholas W Zinck, Danielle Stanton-Turcotte, Emily A Witt, Marley Blommers, Angelo Iulianella","doi":"10.1387/ijdb.240249ai","DOIUrl":"https://doi.org/10.1387/ijdb.240249ai","url":null,"abstract":"The development of cranial nerves, including the trigeminal nerve, and the formation of neuromuscular junctions (NMJs) are crucial processes for craniofacial motor function. Mllt11/Af1q/Tcf7c (hereafter Mllt11), a novel type of cytoskeletal-interacting protein, has been implicated in neuronal migration and neuritogenesis during central nervous system development. However, its role in peripheral nerve development and NMJ formation remains poorly understood. This study investigates the function of Mllt11 during trigeminal ganglion development and its impact on motor innervation of the masseter muscle. We report Mllt11 expression in the developing trigeminal ganglia, suggesting a potential role in cranial nerve development. Using a conditional knockout mouse model to delete Mllt11 in Wnt1-expressing neural crest cells, we assessed trigeminal ganglion development and innervation of the masseter muscle in the jaw. Surprisingly, we found that Mllt11 loss does not affect the initial formation of the trigeminal ganglion but disrupts its placodal vs. neural crest cellular composition. Furthermore, we showed that conditional inactivation of Mllt11 using Wnt1Cre2 led to a reduction of neurofilament density and NMJs within the masseter muscle, along with a reduction of Phox2b+ branchiomotor neurons in rhombomere 2, indicating altered trigeminal motor innervation. This was due to the surprising finding that the Wnt1Cre2/+ mouse driver promoted aberrant recombination and reporter gene expression within branchiomotor neuron pools in rhombomere 2, as well as targeting neural crest cell populations. Our findings show that Mllt11 regulates the cellular composition of the trigeminal ganglion and is essential for proper trigeminal motor innervation in the masseter muscle.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of somatic testicular cells during human development: fetal, peripubertal, adolescent and adult human testis from healthy and infertility related disease. 人类发育过程中体细胞睾丸细胞的特征：来自健康和不孕症相关疾病的胎儿、青春期、青少年和成人睾丸。

IF 1.3

The International journal of developmental biology Pub Date : 2025-06-13 DOI: 10.1387/ijdb.250005ce

Myriam Martin-Inaraja, Lara Herrera, Silvia Santos, Maria Diaz-Nuñez, Antonia Exposito, Roberto Matorras, Maria Begoña Prieto, Susana M Chuva de Sousa Lopes, Cristina Eguizabal

{"title":"Characterization of somatic testicular cells during human development: fetal, peripubertal, adolescent and adult human testis from healthy and infertility related disease.","authors":"Myriam Martin-Inaraja, Lara Herrera, Silvia Santos, Maria Diaz-Nuñez, Antonia Exposito, Roberto Matorras, Maria Begoña Prieto, Susana M Chuva de Sousa Lopes, Cristina Eguizabal","doi":"10.1387/ijdb.250005ce","DOIUrl":"10.1387/ijdb.250005ce","url":null,"abstract":"The transcription factor GATA4 is found in Sertoli and Leydig cells, whereas SOX9 is exclusive to Sertoli cells, being both factors essential for the normal development of murine and human fetal testis. In turn, the steroidogenic acute regulatory protein (STAR) is specifically expressed in Leydig cells. Nevertheless, the function of STAR, GATA4 and SOX9 in peripubertal, adolescent and adult testes in Klinefelter syndrome and azoospermic patients remains poorly understood. To characterize the developmental expression of STAR, GATA4 and SOX9 in human testicular somatic cells, we performed immunofluorescence using fetal, peripubertal, adolescent and adult testes. Our findings demonstrate that STAR is absent in early fetal stages, but present in Leydig cells from 12 weeks of gestation, as well as in peripubertal, adolescent and adult Klinefelter patients, in the adult testis with idiopathic azoospermia and in men showing normal spermatogenesis. GATA4 was expressed in both Sertoli and Leydig cells during all the studied developmental stages and in peripubertal, adolescent and adult patients with and without spermatogenesis. SOX9 was mainly expressed in Sertoli cells in fetal, peripubertal, adolescent and adult Sertoli cell patients. In patients with Klinefelter syndrome as well as in men with or without spermatogenesis SOX9 was also found in Leydig cells. Our findings support the premise that STAR is a key steroidogenic protein for androgen development in the fetal testis, that GATA4 regulates Sertoli and Leydig cells during testis development and that SOX9 regulates the development of Sertoli cells and is present in the Leydig cells of patients with azoospermia.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 2","pages":"91-99"},"PeriodicalIF":1.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tension-induced enhancement of SIX1 expression during preplacodal ectoderm differentiation from human induced pluripotent stem cells. 紧张诱导的SIX1表达在人诱导多能干细胞胎盘前外胚层分化过程中的增强。

IF 1.3

The International journal of developmental biology Pub Date : 2025-06-13 DOI: 10.1387/ijdb.240212tm

Seungtae Kim, Ayumi Horikawa, Takayoshi Yamamoto, Tatsuo Michiue

{"title":"Tension-induced enhancement of SIX1 expression during preplacodal ectoderm differentiation from human induced pluripotent stem cells.","authors":"Seungtae Kim, Ayumi Horikawa, Takayoshi Yamamoto, Tatsuo Michiue","doi":"10.1387/ijdb.240212tm","DOIUrl":"10.1387/ijdb.240212tm","url":null,"abstract":"Based on observations of in vivo morphogenesis, differentiation is expected to be regulated by mechanical cues. However, the detail mechanisms remain largely unknown. A previous study using human pluripotent stem cells (hPSCs) demonstrated that neural plate border (NPB) specification was enhanced by mechanical force. However, it is unknown whether mechanical force is also involved in the specification of the preplacodal ectoderm (PPE), which is derived from the NPB. Here, we verified the validity of the PPE induction method in stretch chambers, and conducted the stretching stimuli experiments. When repetitive stretching stimuli were applied from Day 2 to 10 or Day 2 to 7, expression of the PPE marker SIX1 was increased. However, this increase was not observed when the stimuli were applied from Day 5 to 10, suggesting there is a critical period of sensitivity to mechanical forces. Immunofluorescent staining revealed lower active β-catenin signals in the cell sheet in the stretched samples compared to those in the controls, suggesting a negative correlation between stretching stimuli and Wnt signaling. Our finding suggests that mechanical force is important in PPE differentiation.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 2","pages":"61-69"},"PeriodicalIF":1.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TLR7 expression patterns in mouse eye development and adult ocular tissues. TLR7在小鼠眼发育和成人眼组织中的表达模式。

IF 1.3

The International journal of developmental biology Pub Date : 2025-06-13 DOI: 10.1387/ijdb.240229fa

Marco Rasile, Michele Sommariva, Elena Menegola, Francesca Di Renzo, Martina Anselmi, Lucia Sfondrini, Isabella Barajon, Francesca Arnaboldi

引用次数: 0

RNA and proteins extracted from the regenerating tail of lizards determine inhibition of cancer cell proliferation in vitro. 从蜥蜴再生尾巴中提取的RNA和蛋白质决定了癌细胞增殖的抑制作用。

IF 1.3

The International journal of developmental biology Pub Date : 2025-06-13 DOI: 10.1387/ijdb.250040la

Nicola Greco, Maurizio Onisto, Lorenzo Alibardi

{"title":"RNA and proteins extracted from the regenerating tail of lizards determine inhibition of cancer cell proliferation in vitro.","authors":"Nicola Greco, Maurizio Onisto, Lorenzo Alibardi","doi":"10.1387/ijdb.250040la","DOIUrl":"10.1387/ijdb.250040la","url":null,"abstract":"Recent studies suggest that tail regeneration in lizards begins with a tumor-like stage usually termed regenerative blastema. Oncogenes and tumor suppressors are activated in blastema cells, resulting in a balanced cell proliferation that does not turn the blastema into a tumor. This outgrowth elongates forming new tissues and tail. We previously showed that physiological extracts from regenerating lizard tissues inhibit the growth of cancer cells in vitro within 2-4 days of administration, demonstrating that the growing lizard blastema contains regulatory molecules which can also influence human cancer cells. The molecules responsible for this inhibition were not identified in that initial study. In the present experimental study, after specific extractions of RNAs and/or proteins from the regenerating tail of lizard, we have confirmed the inhibition of breast cancer cell vitality in vitro within 2-3 days from their addition to the culture medium. Proteolysis or heat denaturation of proteins abolished the inhibitory effect. RNA delivered to breast cancer cells in vitro through lipid vesicles (liposomes) showed the highest inhibition of cancer cells vitality. Cell degeneration, detected by microscopy, revealed that RNA is more effective than proteins extracted from regenerating tissues. The present observations further suggest that RNAs coding for known tumor suppressor proteins, and non-coding RNAs that are highly expressed in the regenerating tail, may be key inhibitors (tumor suppressors) of blastema and cancer cell proliferation. The evolution of a mechanism for the self-remission of tumor growth in lizards remains uncertain, but continuing study of this reptile may help uncover natural mechanisms for tumor growth inhibition.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 2","pages":"71-79"},"PeriodicalIF":1.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promoter strength delimits enhancer threshold in the early Drosophila embryo. 启动子强度决定了早期胚胎的增强子阈值。

IF 1.3

The International journal of developmental biology Pub Date : 2025-06-13 DOI: 10.1387/ijdb.240230jh

Miroo Hong, Joung-Woo Hong

{"title":"Promoter strength delimits enhancer threshold in the early Drosophila embryo.","authors":"Miroo Hong, Joung-Woo Hong","doi":"10.1387/ijdb.240230jh","DOIUrl":"10.1387/ijdb.240230jh","url":null,"abstract":"The enhancer threshold is defined as the minimum concentration of transcription factors (TFs) required to elicit an enhancer response in a given time and space. Here, evidence is presented that the enhancer threshold is relative to promoter strength in the early Drosophila embryo. The apparently inactive even-skipped (eve) minimal stripe element (MSE), in which a single Hunchback (Hb)-binding site is deleted, is functionally complemented by the hsp70 promoter in transgenic embryos. Forced pause release of RNA polymerase II (Pol II) and transcription bubble assays show that both eve and heat shock protein 70 (hsp70) promoters exhibit paused Pol II. However, bioinformatics analyses and transient transfection assays indicate that the strength of the hsp70 promoter is much stronger than that of the eve promoter. Consistently, inactive MSE function is also restored by promoters stronger than the eve promoter. It is conceivable that the functional complementarity between enhancer and promoter strengths defines the enhancer threshold, thus determining whether a genomic locus acts as an enhancer for a particular promoter.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 2","pages":"81-90"},"PeriodicalIF":1.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melastatin family Transient Receptor Potential channels support spermatogenesis in planarian flatworms. Melastatin家族瞬时受体电位通道支持涡虫精子发生。

IF 1.3

The International journal of developmental biology Pub Date : 2025-01-01 DOI: 10.1387/ijdb.240180lr

Haley Nicole Curry, Roger Huynh, Labib Rouhana

{"title":"Melastatin family Transient Receptor Potential channels support spermatogenesis in planarian flatworms.","authors":"Haley Nicole Curry, Roger Huynh, Labib Rouhana","doi":"10.1387/ijdb.240180lr","DOIUrl":"10.1387/ijdb.240180lr","url":null,"abstract":"The Transient Receptor Potential superfamily of proteins (TRPs) form cation channels that are abundant in animal sensory systems. Amongst TRPs, the Melastatin-related family (TRPMs) is composed of members that respond to temperature, pH, sex hormones, and various other stimuli. Some TRPMs exhibit enriched expression in the gonads of vertebrate and invertebrate species, but their contributions to germline development remain to be determined. We identified twenty-one potential TRPMs in the planarian flatworm Schmidtea mediterranea and analyzed their anatomical distribution of expression by whole-mount in situ hybridization. Enriched expression of two TRPMs (Smed-TRPM-c and Smed-TRPM-l) was detected in testis, whereas eight TRPM genes had detectable expression in patterns representative of neuronal and/or sensory cell types. Functional analysis of TRPM homologs by RNA-interference (RNAi) revealed that disruption of normal levels of Smed-TRPM-c expression impaired sperm development, indicating a role for this receptor in supporting spermatogenesis. Smed-TRPM-l RNAi alone did not result in a detectable phenotype, but it did increase sperm development deficiencies when combined with Smed-TRPM-c RNAi. Fluorescence in situ hybridization revealed expression of Smed-TRPM-c in early spermatogenic cells within testes, suggesting cell-autonomous regulatory functions in germ cells for this gene. In addition, Smed-TRPM-c RNAi resulted in reduced numbers of presumptive germline stem cell clusters in asexual planarians, suggesting that Smed-TRPM-c supports the establishment, maintenance, and/or expansion of spermatogonial germline stem cells. While further research is needed to identify the factors that trigger Smed-TRPM-c activity, these findings reveal one of the few known examples for TRPM function in the direct regulation of sperm development.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 1","pages":"21-34"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three Decades of the Spanish Society for Developmental Biology (SEBD): Insights and Emerging Perspectives from the 18th Spanish Society for Developmental Biology Meeting (SEBD 2024). 三十年的西班牙社会发展生物学（SEBD）：从第18届西班牙社会发展生物学会议（SEBD 2024）的见解和新兴观点。

IF 1.3

The International journal of developmental biology Pub Date : 2025-01-01 DOI: 10.1387/ijdb.250034sa

Eloisa Herrera, Sandra Acosta, María Almuedo, Victor Borrell, Cristian Cañestro, Sergio Casas-Tintó, Luis M Escudero, Nicole Gorfinkiel, Esteban Hoijman, José Carlos Pastor-Pareja, Barbara Pernaute, Teresa Rayón, Murielle Saade, Jordi Solana, Vikas Trivedi, Elisa Martí, Cristina Pujades, Sofia J Araújo

引用次数: 0

Inhibition of COX2 impairs angiogenesis and causes vascular defects in developing zebrafish embryos. 抑制COX2损害血管生成并导致发育中的斑马鱼胚胎血管缺陷。

IF 1.3

The International journal of developmental biology Pub Date : 2025-01-01 DOI: 10.1387/ijdb.240222sb

Lakshmi Pillai, Vishakha Nesari, Dhanush Danes, Suresh Balakrishnan

{"title":"Inhibition of COX2 impairs angiogenesis and causes vascular defects in developing zebrafish embryos.","authors":"Lakshmi Pillai, Vishakha Nesari, Dhanush Danes, Suresh Balakrishnan","doi":"10.1387/ijdb.240222sb","DOIUrl":"10.1387/ijdb.240222sb","url":null,"abstract":"This study investigated the role of cyclooxygenase-2 (COX2) in angiogenesis during zebrafish embryogenesis by inhibiting COX2 activity with etoricoxib. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis confirmed the successful penetration of etoricoxib into zebrafish embryos, leading to selective inhibition of COX2 without affecting COX1 activity. COX2 inhibition caused a significant reduction in prostaglandin E2 levels throughout development. Phenotypically, treated embryos exhibited pericardial edema, bradycardia, and defective vascular development, including delays in intersegmental vessel (ISV) sprouting, incomplete dorsal longitudinal anastomotic vessel (DLAV) formation by 48 hpf, and impaired vascular networks by 72 hpf. Confocal imaging and AngioTool analysis revealed reduced vessel length, area and increased lacunarity. Molecular analysis showed significant downregulation of vascular endothelial growth factor A (vegfa), kdr, pi3k and akt transcripts, as well as reduced VEGFA, EP4 and Akt protein levels, disrupting VEGFA-PI3K-Akt signaling. Additionally, reduced expression of ephrinb and prox1 affected arterial and venous identity formation. These results demonstrate that COX2 is essential for proper angiogenesis during zebrafish development, and its inhibition leads to significant vascular defects, underscoring COX2's crucial role in regulating VEGFA-mediated angiogenesis.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"11-20"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0