The International journal of developmental biology最新文献_第8页

Human transient receptor potential (TRP) channel expression profiling in carcinogenesis. 人瞬时受体电位(TRP)通道在癌变中的表达谱。

The International journal of developmental biology Pub Date : 2015-11-19 DOI: 10.1387/ijdb.150232dg

M. Bernardini, A. Fiorio Pla, N. Prevarskaya, D. Gkika

引用次数: 25

Ion currents involved in gamete physiology. 离子电流与配子生理有关。

The International journal of developmental biology Pub Date : 2015-11-19 DOI: 10.1387/ijdb.150202et

A. Gallo, E. Tosti

引用次数: 18

Ionic messengers in development and cancer. 发育和癌症中的离子信使。

The International journal of developmental biology Pub Date : 2015-11-19 DOI: 10.1387/ijdb.150215mm

M. Moreau, C. Leclerc

引用次数: 1

Two-Pore Channel 2 activity is required for slow muscle cell-generated Ca(2+) signaling during myogenesis in intact zebrafish. 在完整斑马鱼的肌肉形成过程中，双孔通道2的活性是缓慢的肌肉细胞产生的Ca(2+)信号所必需的。

The International journal of developmental biology Pub Date : 2015-11-19 DOI: 10.1387/ijdb.150206am

Jeffrey J. Kelu, Hayley Chan, S. Webb, Arthur H. Cheng, M. Ruas, J. Parrington, A. Galione, A. Miller

{"title":"Two-Pore Channel 2 activity is required for slow muscle cell-generated Ca(2+) signaling during myogenesis in intact zebrafish.","authors":"Jeffrey J. Kelu, Hayley Chan, S. Webb, Arthur H. Cheng, M. Ruas, J. Parrington, A. Galione, A. Miller","doi":"10.1387/ijdb.150206am","DOIUrl":"https://doi.org/10.1387/ijdb.150206am","url":null,"abstract":"We have recently characterized essential inositol 1,4,5-trisphosphate receptor (IP 3R) and ryanodine receptor (RyR)-mediated Ca(2+) signals generated during the differentiation of slow muscle cells (SMCs) in intact zebrafish embryos. Here, we show that the lysosomal two-pore channel 2 (TPC2) also plays a crucial role in generating, and perhaps triggering, these essential Ca(2+) signals, and thus contributes to the regulation of skeletal muscle myogenesis. We used a transgenic line of zebrafish that expresses the bioluminescent Ca(2+) reporter, aequorin, specifically in skeletal muscle, in conjunction with morpholino (MO)-based and pharmacological inhibition of TPC2, in both intact embryos and isolated SMCs. MO-based knock-down of TPC2 resulted in a dramatic attenuation of the Ca(2+) signals, whereas the introduction of TPCN2-MO and TPCN2 mRNA together partially rescued the Ca(2+) signaling signature. Embryos treated with trans-ned-19 or bafilomycin A1, a specific NAADP receptor inhibitor and vacuolar-type H(+)ATPase inhibitor, respectively, also displayed a similar disruption of SMC Ca(2+) signaling. TPC2 and lysosomes were shown via immunohistochemistry and confocal laser scanning microscopy to be localized in perinuclear and striated cytoplasmic domains of SMCs, coincident with patterns of IP 3R and RyR expression. These data together imply that TPC2-mediated Ca(2+) release from lysosomes acts upstream from RyR- and IP 3R-mediated Ca(2+) release, suggesting that the former might act as a sensitive trigger to initiate the SR-mediated Ca(2+)-induced-Ca(2+)-release essential for SMC myogenesis and function.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"15 1","pages":"313-25"},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82419039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Inhibition of SOCE disrupts cytokinesis in zebrafish embryos via inhibition of cleavage furrow deepening. 抑制SOCE通过抑制卵裂沟加深破坏斑马鱼胚胎的细胞分裂。

The International journal of developmental biology Pub Date : 2015-11-19 DOI: 10.1387/ijdb.150209sw

C. Chan, Yiyun Chen, T. S. Hung, A. Miller, A. Shipley, S. Webb

{"title":"Inhibition of SOCE disrupts cytokinesis in zebrafish embryos via inhibition of cleavage furrow deepening.","authors":"C. Chan, Yiyun Chen, T. S. Hung, A. Miller, A. Shipley, S. Webb","doi":"10.1387/ijdb.150209sw","DOIUrl":"https://doi.org/10.1387/ijdb.150209sw","url":null,"abstract":"During the first few cell division cycles in zebrafish, distinct Ca(2+) transients are localized to the early embryonic cleavage furrows, where they accompany (and are required for) furrow positioning, propagation, deepening and apposition. It has previously been shown that the endoplasmic reticulum (ER) acts as the primary store for generating these Ca(2+) transients via release through inositol 1,4,5-trisphosphate receptors (IP 3Rs). We hypothesised that maintaining the elevated levels of intracellular Ca(2+) required for deepening and apposition of the cleavage furrows in these large eggs might result in the depletion of the available ER Ca(2+) store, thus the role of store-operated Ca(2+) entry (SOCE) was examined. Newly fertilized, dechorionated embryos were incubated with various SOCE inhibitors, starting just prior to the onset of the first cell division cycle. The effect of these inhibitors on mitosis, furrow positioning, propagation, deepening and apposition, and the generation of the cytokinetic Ca(2+) transients was determined. Treatment with 2-APB or SKF 96365 had no major effect on mitosis, furrow positioning or propagation, but inhibited furrow deepening resulting in regression of the cleavage furrow. Both of these inhibitors also blocked the furrowing Ca(2+) transient, with SKF 96365 having a more profound inhibitory effect than 2-APB. In zebrafish, SOCE does not appear to be required for mitosis or the early stages of cytokinesis during the early embryonic cell division cycles, but it does appear to be essential for maintaining the elevated levels of [Ca(2+)]i for the extended periods that are required during furrow deepening and daughter cell apposition.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"29 1","pages":"289-301"},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85001242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150310ht

Cheng-Yung Lin, Hsing-Yen Huang, Po-nien Lu, Chien-Wei Lin, Kuan-Ming Lu, H. Tsai

{"title":"Ras-Related Nuclear Protein is required for late developmental stages of retinal cells in zebrafish eyes.","authors":"Cheng-Yung Lin, Hsing-Yen Huang, Po-nien Lu, Chien-Wei Lin, Kuan-Ming Lu, H. Tsai","doi":"10.1387/ijdb.150310ht","DOIUrl":"https://doi.org/10.1387/ijdb.150310ht","url":null,"abstract":"Ras-related nuclear protein (Ran) is involved in cell division by regulating nucleocytoplasmic transport and modulating the assembly of tubulin. However, its function in embryonic development is unclear. We used zebrafish to study the roles of Ran in eye development. The ran transcripts were restrictedly expressed in head and eyes after the pharyngula stage. The microphthalmos, in which no ordered layers with differentiated retinal cells were detected, was observed in the ran-deficient embryos. They exhibited faster decline cyclinD1-expressed cells, suggesting that cell cycle regulation in retinae was defective. The apoptotic signals in the retinae of ran-deficient embryos remained low at early (24 hpf) stage. Early eye field specification markers, rx1 and pax6, were only slightly affected, and markers for establishing axon migration, fgf8 and pax2, were normally expressed, suggesting Ran is not required in the early stages of eye development. However, the early optic nerve differentiation marker p57kip2 was not expressed at middle (48 hpf) and late (72 hpf) stages. We also observed a decrease in the retinal neuron proteins HuC and Neurolin. The proneural gene ath5, which first determines the cell fate of the developing ganglion cell layer, was undetectable. Furthermore, we found that Ran was associated with ADP-ribosylation factor-like protein 6-interacting protein 1 (Arl6ip1), which plays a role in retinal development, suggesting that Ran associates with Arl6ip1 to regulate retinal development. Therefore, while the effects of Ran are minimal during early specification of the eye field, Ran is required for proliferation and differentiation of retinal cells at later developmental stages.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"96 1","pages":"435-42"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80216455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Angiogenesis and hyperbaric oxygen in the chick embryo chorioallantoic membrane. 鸡胚绒毛膜尿囊膜的血管生成与高压氧作用。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150280dr

U. Montecorboli, T. Annese, C. Marinaccio, D. Ribatti

{"title":"Angiogenesis and hyperbaric oxygen in the chick embryo chorioallantoic membrane.","authors":"U. Montecorboli, T. Annese, C. Marinaccio, D. Ribatti","doi":"10.1387/ijdb.150280dr","DOIUrl":"https://doi.org/10.1387/ijdb.150280dr","url":null,"abstract":"Hyperbaric Oxygen Therapy (HBOT) is increasingly applied in different areas of medical practice. The oxy-hyperbarism effects are not well understood in cancer malignancy. One unique feature of cancer is the presence of hypoxic regions that are insensitive to conventional therapies. It is possible to alter the hypoxic state and produce reactive oxygen species for better treatment outcome by HBOT. In the present study, we determined the effects of HBOT on angiogenesis, a signature of cancer progression, by using the chick chorioallantoic membrane (CAM) in vivo assay. CAMs were exposed to 2.0 ATA (atmospheres absolute) for 30 min of hyperbaric oxygen on the 6(th) and 7(th) days of incubation (ED6, ED7). On the 10-11(th) day of incubation, CAMs were excised from eggs, fixed and analysed using APERIO ImageScope software. HBOT outcomes were evaluated quantifying the volumetric area occupied by blood vessels and calculating the number of blood vessel ramifications. Results indicated that CAMs treated at ED6 and ED7 had a significantly higher CAM vascularization and an increased number of blood vessel ramifications (+82% higher for ED6) compared to untreated CAMs (ED6=63.3±2.5 and ED7=57.7±5.5 vs. CTRL=34.7±2.5). Thus, HBOT induces an angiogenic response in treated CAMs through a classic sprouting mechanism.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"59 10-12 1","pages":"461-4"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87923269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Developmental expression of the N-myc downstream regulated gene (Ndrg) family during Xenopus tropicalis embryogenesis. 热带非洲爪蟾胚胎发生过程中N-myc下游调控基因(Ndrg)家族的发育表达。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150178xh

Chao Zhong, Yan-Kuan Zhou, Shan-shan Yang, Jun-Fang Zhao, Xiao-long Zhu, Hen-Huang Chen, Peichao Chen, Liquan Huang, Xiao Huang

{"title":"Developmental expression of the N-myc downstream regulated gene (Ndrg) family during Xenopus tropicalis embryogenesis.","authors":"Chao Zhong, Yan-Kuan Zhou, Shan-shan Yang, Jun-Fang Zhao, Xiao-long Zhu, Hen-Huang Chen, Peichao Chen, Liquan Huang, Xiao Huang","doi":"10.1387/ijdb.150178xh","DOIUrl":"https://doi.org/10.1387/ijdb.150178xh","url":null,"abstract":"The N-myc downstream regulated gene (Ndrg) family consists of four main members Ndrg1, 2, 3, and 4. The Ndrg genes are involved in many vital biological events including development. However, comprehensive expression patterns of this gene family during vertebrate embryogenesis remain largely unknown. Here, we analyzed the Ndrg family from the evolutionary perspective and examined the expression patterns of the Ndrg genes during Xenopus tropicalis embryogenesis. Different Ndrg family members of vertebrates are separated into different homology clusters which can be further classified into two groups and each Ndrg family member is well conserved during evolution. The temporal and spatial expression patterns of Ndrg1, 2, 3 and 4 are different during early Xenopus tropicalis development. Ndrg1, 2 and 4 are maternally expressed genes while Ndrg3 is a zygotically expressed gene. The Ndrg genes are differentially expressed in the developing central nervous system, the developing sensory organs, and the developing excretory organs. Moreover, they also show other specific expression domains. Our results indicate that the Ndrg genes exhibit specific expression patterns and may play different roles during vertebrate embryogenesis.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"68 1","pages":"511-7"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87426169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

T-cell internal antigen 1 counteracts somatic RNA degradation during early Xenopus embryogenesis. 在非洲爪蟾胚胎发生早期，t细胞内抗原1抵消体细胞RNA降解。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150137db

Diana Bauermeister, Maike Claussen, T. Pieler

{"title":"T-cell internal antigen 1 counteracts somatic RNA degradation during early Xenopus embryogenesis.","authors":"Diana Bauermeister, Maike Claussen, T. Pieler","doi":"10.1387/ijdb.150137db","DOIUrl":"https://doi.org/10.1387/ijdb.150137db","url":null,"abstract":"In Xenopus laevis, maternal transcripts that localize to the vegetal cortex of the oocyte are specifically inherited by prospective germ cells during cleavage stages. While a large fraction of maternal transcripts is degraded during the maternal to zygotic transition (MZT), transcripts associated with the germ-line are stable. A sequence in the dead end 1 3'UTR mediates vegetal localization in the oocyte as well as miR mediated clearance in somatic cells and germ cell specific stabilization during the MZT in embryos. We could identify Tia1 to co-precipitate with known components of vegetal localization RNPs in X. laevis oocytes. Tia1 interacts and co-localizes with various localization elements from vegetally localizing RNAs. In X. laevis embryos, ectopic expression of Tia1 counteracts somatic degradation of dnd1 localization element reporter RNAs and it can synergize with Dnd1 protein in reporter RNA stabilization. Ectopic Tia1 also protects several endogenous localizing and germ cell specific mRNAs from somatic degradation. Thus, proteins that protect germ-line transcripts from miR mediated decay during the MZT in embryos might bind these RNAs already in the oocyte.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"1 1","pages":"425-33"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89435839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

External ear microRNA expression profiles during mouse development. 小鼠发育过程中外耳microRNA表达谱。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150124sf

Leda Torres, Ulises Juárez, Laura García, J. Miranda-Ríos, S. Frías

{"title":"External ear microRNA expression profiles during mouse development.","authors":"Leda Torres, Ulises Juárez, Laura García, J. Miranda-Ríos, S. Frías","doi":"10.1387/ijdb.150124sf","DOIUrl":"https://doi.org/10.1387/ijdb.150124sf","url":null,"abstract":"MicroRNAs (miRNAs) comprise a class of approximately 22 nucleotide regulatory non-coding RNAs that play several roles in diverse biological processes. Recent reports suggest that embryonic development in mammals is accompanied by dynamic changes in miRNA expression; however, there is no information regarding the role of miRNAs in the development of the external ear. The aim of this study was to determine the stage-specific expression of miRNAs during mouse external ear development in order to identify potentially implicated miRNAs along with their possible targets. miRNA expression profiles from fetal mice pinnae and back skin tissues at 13.5 dpc and 14.5 dpc were obtained using an Affymetrix GeneChip miRNA 3.0 array. Biological triplicates for both tissues, each collected from a litter averaging 16 fetuses, were analyzed. The results were analyzed with Affymetrix's Transcriptome Analysis Console software to identify differentially expressed miRNAs. We observed differential expression of 40 miRNAs including some predicted to target genes implicated in external ear development, such as mmu-miR-10a, an miRNA known to modulate Hoxa1 mRNA levels, and mmu-miR-200c and mmu-miR-205. To our knowledge, this is the first miRNA expression profiling study of external ear development in mammals. These data could set the basis to understand the implications of miRNAs in normal external ear development.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"4 1","pages":"497-503"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87614753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10