MPF,海星卵母细胞和无细胞提取物的背景-对岸本武夫的采访。

J. Kubiak, T. Kishimoto
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引用次数: 0

摘要

岸本武夫教授的研究对细胞周期领域产生了巨大的影响。虽然他最喜欢的模型一直是海星卵母细胞,但他在研究中也使用了许多其他模式生物。在他的实验室里,无细胞提取物作为回答细胞周期研究问题的一种非常有用的工具被广泛使用。最近,岸本教授发现了多年来被认为是细胞周期蛋白依赖性激酶1 (CDK1)的m期促进因子(MPF)的身份。然而,Takeo Kishimoto发现MPF实际上由两种激酶组成:CDK1和Greatwall激酶。当CDK1磷酸化有丝分裂底物时,Greatwall激酶允许这些底物持续处于磷酸化状态,因为它调节磷酸酶PP2A,它使大多数CDK1底物去磷酸化。当我开始采访岸本教授时,我最感兴趣的是他在无细胞提取物方面的经验。然而,正如你将在下文看到的,我们几乎立即转向强积金的身份问题。这是完全可以理解的,因为MPF的身份似乎是武夫科学生涯的主要兴趣所在。我希望读者会喜欢这次采访,并能够了解科学研究的许多方面,这些方面通常不会出现在正规的研究论文中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MPF, starfish oocyte and cell-free extract in the background - an interview with Takeo Kishimoto.
Professor Takeo Kishimoto's research has an enormous impact on the cell cycle field. Although his favorite model has always been a starfish oocyte, he has used many other model organisms in his research. Cell-free extracts have been wildly used in his laboratory as a very useful tool to answer cell cycle research questions. Recently, professor Kishimoto discovered the identity of the M-phase promoting factor (MPF) that was thought for years to be cyclin-dependent kinase 1 (CDK1). However, Takeo Kishimoto found that MPF consists in fact of two kinases: CDK1 and Greatwall kinase. While CDK1 phosphorylates mitotic substrates, Greatwall kinase allows these substrates to persist in their phosphorylated state because it regulates phosphatase PP2A, which dephosphorylates the majority of CDK1 substrates. When I started to interview Prof. Kishimoto, I was mostly interested in his experiences with cell-free extracts. However, as you will see below we almost immediately turned to the problem of the identity of MPF. This is fully understandable because the identity of MPF seems to be a major interest in Takeo's scientific career. I hope readers will enjoy this interview and will be able to learn about many aspects of scientific research, which do not usually appear in regular research papers.
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