Acta cirurgica brasileira最新文献_第4页

Brazilian authorship gender trends on academic surgery: a bigdata analysis. 巴西学术外科的作者性别趋势：大数据分析。

Acta cirurgica brasileira Pub Date : 2024-11-29 eCollection Date: 2024-01-01 DOI: 10.1590/acb397724

Ana Kim, Luana Baptistele Dornelas, Luiza Telles, Ayla Gerk, Sarah Bueno Motter, Sarah Lopes Salomão, David Mooney, Cristina Camargo, Roseanne Ferreira

{"title":"Brazilian authorship gender trends on academic surgery: a bigdata analysis.","authors":"Ana Kim, Luana Baptistele Dornelas, Luiza Telles, Ayla Gerk, Sarah Bueno Motter, Sarah Lopes Salomão, David Mooney, Cristina Camargo, Roseanne Ferreira","doi":"10.1590/acb397724","DOIUrl":"10.1590/acb397724","url":null,"abstract":"Purpose: To evaluate the gender distribution of first and last authors with Brazilian surgical affiliations in PubMed-indexed surgical journals.Methods: Data from eligible surgical journals were retrieved using Scimago Journal & Country Rank 2021 and manually reviewed. Manuscripts published from 2018 to 2022 were included if at least one author was affiliated with a Brazilian institution and a surgical specialty.Results: Data from 340 eligible surgical journals were included. We analyzed first and last authors' forenames of 1,881 manuscripts. Women comprised 16.7% of the first and 12.4% of the last authors. Analyzing the differences in gender trends in authorship across the five Brazilian regions, we found that the South had the highest representation, while the Midwest and North showed the lowest, respectively. Obstetrics and gynecology featured the highest percentage of women-first authors, whereas orthopedics had the lowest. For the last authorship, pediatric surgery showed the highest, with hand surgery having the lowest representation. Male first authors were 1.9 times more likely to engage in international collaborations.Conclusions: This study suggests the persistent underrepresentation of Brazilian women in surgical journal authorship. Local policy changes should be considered to encourage greater diversity and inclusivity in surgical research.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e397724"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11606619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cesarean scar dehiscence in early puerperium and influence of barbed suture: tridimensional ultrasound evaluation in a randomized clinical study. 产褥期早期剖宫产瘢痕裂开及刺缝线的影响：随机临床研究中的三维超声评价。

Acta cirurgica brasileira Pub Date : 2024-11-29 eCollection Date: 2024-01-01 DOI: 10.1590/acb399124

Newton de Paula Ishikawa, Gabriela Ewerling Souza, Thays Andressa Albuquerque Monteiro, Albert Schiaveto de Souza, Ricardo Dutra Aydos, Durval Batista Palhares

{"title":"Cesarean scar dehiscence in early puerperium and influence of barbed suture: tridimensional ultrasound evaluation in a randomized clinical study.","authors":"Newton de Paula Ishikawa, Gabriela Ewerling Souza, Thays Andressa Albuquerque Monteiro, Albert Schiaveto de Souza, Ricardo Dutra Aydos, Durval Batista Palhares","doi":"10.1590/acb399124","DOIUrl":"10.1590/acb399124","url":null,"abstract":"Purpose: This study investigated the hypothesis of early dehiscence of hysterorrhaphy as the initial stage of post-cesarean uterine scar defects, examining the possible influence of barbed suture in this process.Methods: This longitudinal, prospective, double-blind study included 54 pregnant women with no history of cesarean section, randomized into two suture groups: #0 polyglactin or #1 barbed PDS threads. Sutures were continuous, unlocked, involved the entire myometrium in a single layer, and included the endometrium. Tridimensional transvaginal ultrasonography was performed on the second day postpartum to investigate scar dehiscence and measure its maximal width.Results: The groups had 29 and 25 participants, respectively. Ages: 18-37 (mean 25.80 ± standard error of the mean 0.69) years old. Groups were homogeneous for labor duration, cervical thickness, edge-to-os distance, retroversion, amniotic sac rupture, and additional hemostatic sutures required. Uterine retroversion accounted for 7.4% of cases. Dehiscence was observed in 68.5% (3.98 ± 0.57 mm). The only factor correlating (positively) with dehiscence width was myometrial thickness, whether proximal or distal.Conclusions: Suture type had no influence on early dehiscence, which occurred at the same rate as published niche formation rates. Tridimensional ultrasound proved effective for evaluating dehiscence.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e399124"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11606612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research trends in pediatric splenic trauma in Brazil: how much has changed in the last two decades? 巴西小儿脾损伤的研究趋势：在过去的二十年里发生了多大的变化？

Acta cirurgica brasileira Pub Date : 2024-11-29 eCollection Date: 2024-01-01 DOI: 10.1590/acb399524

Luiza Telles, Ayla Gerk, Ana Maria Diniz, Madeleine Carroll, Sarah Lopes, Ana Kim, Brenda Feres, Anna Fontes, Roseanne Ferreira, Joaquim Bustorff-Silva, David P Mooney

{"title":"Research trends in pediatric splenic trauma in Brazil: how much has changed in the last two decades?","authors":"Luiza Telles, Ayla Gerk, Ana Maria Diniz, Madeleine Carroll, Sarah Lopes, Ana Kim, Brenda Feres, Anna Fontes, Roseanne Ferreira, Joaquim Bustorff-Silva, David P Mooney","doi":"10.1590/acb399524","DOIUrl":"10.1590/acb399524","url":null,"abstract":"Purpose: Research in high-income countries has extensively documented the non-operative management of spleen injuries in children, resulting in low splenectomy rates (5%). However, there is a lack of literature on this topic in low- and-middle-income countries (LMICs), including Brazil. This scoping review analyzed pediatric spleen trauma research trends in Brazil and the United States of America (USA).Methods: Search strategy was conducted across five databases, considering articles published in English or Portuguese from January 1968 to 2023 that reported spleen injury in patients younger than 18 years old in Brazil or the USA. Two pairs of independent reviewers screened the title and the abstract, followed by a full-text review.Results: The total of 7,150 studies was identified, of which 295 were eligible for data extraction. Most papers (98.64%, 301) originated from the USA, while only 1.36% (4) were from Brazil. In addition, 46.44% (137) articles reported intrabdominal injury, including splenic trauma, 16.27% (48) liver and spleen injury, and 37.29% (110) reported isolated spleen injury. The operative rate for spleen injury was 11.33% in American studies (40,812/359,926) compared to 98.57% (137/139) in Brazilian studies.Conclusions: Brazil contributed only with four studies on pediatric splenic trauma over two decades. Future studies should explore the incidence and management of splenic trauma in LMICs.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e399524"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11606610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Results of the use of human platelet-rich fibrin membrane applied on colorrhaphy in nourished and malnourished rats. 使用富含血小板的人纤维蛋白膜对营养不良和营养不良大鼠进行彩超检查的结果。

Acta cirurgica brasileira Pub Date : 2024-11-08 eCollection Date: 2024-01-01 DOI: 10.1590/acb397824

Fábio Henrique de Oliveira, Lucas Silva de Oliveira, Luciana Xavier Pereira, Vinicius Silva Belo, Fernanda Elias Ferreira Rabelo, Guilherme Santos Couto, Antonio Lacerda-Filho

{"title":"Results of the use of human platelet-rich fibrin membrane applied on colorrhaphy in nourished and malnourished rats.","authors":"Fábio Henrique de Oliveira, Lucas Silva de Oliveira, Luciana Xavier Pereira, Vinicius Silva Belo, Fernanda Elias Ferreira Rabelo, Guilherme Santos Couto, Antonio Lacerda-Filho","doi":"10.1590/acb397824","DOIUrl":"10.1590/acb397824","url":null,"abstract":"Purpose: To evaluate the effects of platelet-rich fibrin (PRF) on the healing of intestinal sutures in rats.Methods: Forty rats were distributed into four groups. Two groups were treated with a standard diet and considered nourished (I and II). Two other groups were treated with cornmeal and considered malnourished (III and IV). All animals underwent cecotomy and cecorrhaphy. Groups II and IV had sutures overlapped with human PRF membrane. The following parameters were evaluated: animal weight, death, rupture site, rupture pressure, collagen, and reticulin dosage in the suture line.Results: The use of PRF did not influence deaths, rupture pressure or rupture location. For malnourished animals, a significant difference was observed in relation to the rupture site, corresponding to the suture line (p = 0.038) and reticulin dosage (p = 0.040), when PRF was used. There was no difference in relation to burst pressures.Conclusions: The use of PRF did not influence intestinal healing in nourished rats. In the group of malnourished animals, its use favored healing.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e397824"},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11548133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptotic effect of thymoquinone on OVCAR3 cells via the P53 and CASP3 activation. 胸腺醌通过激活 P53 和 CASP3 对 OVCAR3 细胞产生凋亡效应。

Acta cirurgica brasileira Pub Date : 2024-11-08 eCollection Date: 2024-01-01 DOI: 10.1590/acb399224

Özge Karaosmanoğlu, Zeynep Kamalak, İlhan Özdemir, Şamil Öztürk, Mehmet Cudi Tuncer

{"title":"Apoptotic effect of thymoquinone on OVCAR3 cells via the P53 and CASP3 activation.","authors":"Özge Karaosmanoğlu, Zeynep Kamalak, İlhan Özdemir, Şamil Öztürk, Mehmet Cudi Tuncer","doi":"10.1590/acb399224","DOIUrl":"10.1590/acb399224","url":null,"abstract":"Purpose: The limitations in cancer treatment and the inadequacy of classical methods have made it necessary to discover therapeutics in cancer treatment. The cytotoxicity of thymoquinone, which has quite different properties in terms of biological activities, in ovarian cancer cells, and the changes in the expression levels of apoptotic genes (p53/caspase-3 (casp-3)) were investigated.Methods: In the study, thymoquinone (5, 50, 100, 250 and 500 µM and 24, 48, 72 hours) were applied to ovarian adenocarcinoma cancer cell line (OVCAR3), at different concentrations. Cytotoxic effect of thymoquinone on OVCAR-3 cells were analyzed by MTT method, and apoptotic and pro-apoptotic gene expression levels (p53, Casp-3) of thymoquinone in cancer cells were analyzed by quantitative real-time polymerase chain reaction.Results: Thymoquinone, whose effect has been revealed in many types of cancer, was shown to significantly reduce the viability of OVCAR3 cancer cells depending on the dose and time (p < 0.05). It was also determined that Casp-3 and p53 gene expressions increased in OVCAR3 cells.Conclusions: Considering the in-vitro cytotoxic activity and apoptotic gene expressions of thymoquinone, an important treatment agent, since it is a promising agent for the future of cancer treatment, more comprehensive studies may pave the way for its clinical use.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e399224"},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11548134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of the thyroidectomy training box: cervical simulator for training endoscopic transoral thyroidectomy. 甲状腺切除术训练箱的开发和验证：用于训练内窥镜经口甲状腺切除术的颈部模拟器。

Acta cirurgica brasileira Pub Date : 2024-11-08 eCollection Date: 2024-01-01 DOI: 10.1590/acb397624

Valdenor Neves Feitosa, Taís Vasconcelos Cidrão, Ingrid Arruda Castro, Klayton Coelho de Souza Júnior, Sarah Rodrigues Chaves Martins, Francisco Everton Pereira da Silva, Luiz Gonzaga Moura Junior

{"title":"Development and validation of the thyroidectomy training box: cervical simulator for training endoscopic transoral thyroidectomy.","authors":"Valdenor Neves Feitosa, Taís Vasconcelos Cidrão, Ingrid Arruda Castro, Klayton Coelho de Souza Júnior, Sarah Rodrigues Chaves Martins, Francisco Everton Pereira da Silva, Luiz Gonzaga Moura Junior","doi":"10.1590/acb397624","DOIUrl":"10.1590/acb397624","url":null,"abstract":"Purpose: To develop and validate a trans oral endoscopic thyroidectomy vestibular approach (TOETVA) simulator.Methods: The first phase of the project consisted of designing and developing a transoral thyroid surgery simulator based on real surgeries. The product has the oral cavity for attaching the three trocars and the cervical part containing the thyroid and adjacent structures. In the second phase, the simulator was validated by specialists who performed an endoscopic thyroidectomy procedure. They all filled a questionnaire about the simulator and the simulation based on the Likert scale.Results: The simulator consists of a console similar to a human bust and a high-resolution camera system connected to a 22-inch monitor. The simulator had excellent results in the visual evaluation (face validity), with 100% of responses between good and excellent for the following characteristics: synthetic structures, design, visibility of the surgery field, resistance, resilience, fulcrum effect, ergonomics, surgical material, and practicality. The last three were rated higher, with more votes for excellent. For content validity, the items that received the best ratings were, precisely, the steps relating to the surgical procedure: opening the intermuscular midline, isthmotomy, and thyroidectomy.Conclusions: The thyroidectomy training box showed great ability to simulate a TOETVA, with satisfactory evaluations concerning its visual and content validation.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e397624"},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11548132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robotic surgery versus conventional laparoscopy in colon cancer patients: a systematic review and meta-analysis. 结肠癌患者的机器人手术与传统腹腔镜手术：系统回顾与荟萃分析。

Acta cirurgica brasileira Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.1590/acb397224

Giuliana Fulco Gonçalves, Pedro Vilar de Oliveira Villarim, Vitória Ribeiro Dantas Marinho, Clarissa Amaral Abreu, Luiz Henrique Moreira Pereira, Luiz Henrique Moreira Pereira, Sofia Emerenciano Gurgel, Amália Cínthia Meneses Rêgo, Kleyton Santos de Medeiros, Irami Araújo-Filho

{"title":"Robotic surgery versus conventional laparoscopy in colon cancer patients: a systematic review and meta-analysis.","authors":"Giuliana Fulco Gonçalves, Pedro Vilar de Oliveira Villarim, Vitória Ribeiro Dantas Marinho, Clarissa Amaral Abreu, Luiz Henrique Moreira Pereira, Luiz Henrique Moreira Pereira, Sofia Emerenciano Gurgel, Amália Cínthia Meneses Rêgo, Kleyton Santos de Medeiros, Irami Araújo-Filho","doi":"10.1590/acb397224","DOIUrl":"10.1590/acb397224","url":null,"abstract":"Purpose: To compare robotic versus laparoscopic colectomies in colon cancer patients in general complications.Methods: Nine databases were searched for randomized controlled trials (RCT) investigating patients with colon cancer, submitted to robotic surgery (RS) compared to a laparoscopic (LC) approach. The risk of bias was assessed using RoB 2.0 tool, and certainty of the evidence was evaluated by Grading of Recommendations, Assessment, Development and Evaluation (GRADE). Data synthesis was performed using the software R. The meta-analysis of the included studies was carried out using the fixed-effects model (DerSimonian and Laird). Heterogeneity was measured using I2 analysis.Results: A total of four studies were used with 293 patients. Three studies were used in this comparative LC vs. RS when evaluating infection rates on surgical wound sites. The odds ratio (OR) appeared to be slightly favorable to LC (OR = 3.05; 95% confidence interval-95%CI 0.78-11.96). In the hospitalization rates analysis, two randomized controlled trials were used, and the mean differences slightly favored the RS (MD = -0.54; 95%CI -2.28-1.19). GRADE evaluation detected a serious risk of bias due to RCT format and RoB-2 concurred.Conclusion: Both types of procedures seem to have their own benefits, risks, and limitations. They seem close to equal in terms of postsurgical infection and hospitalization.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e397224"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of postoperative outcomes between different dissection techniques during laparoscopic cholecystectomy in rabbits: randomized study. 比较兔子腹腔镜胆囊切除术中不同解剖技术的术后效果：随机研究。

Acta cirurgica brasileira Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.1590/acb396324

María Camila Maldonado Vera, Monica Carolina Nery Wittmaack, Maria Eduarda Bastos Andrade Moutinho Conceição, Rachel Inamassu Faccini, Guilherme Sembenelli, Gabriel Luiz Montanhim, Mareliza Possa de Menezes, Cléber Kazuo Ido, Luiz Paulo Nogueira Aires, Gabriel João Unger Carra, Paola Castro Moraes

{"title":"Comparison of postoperative outcomes between different dissection techniques during laparoscopic cholecystectomy in rabbits: randomized study.","authors":"María Camila Maldonado Vera, Monica Carolina Nery Wittmaack, Maria Eduarda Bastos Andrade Moutinho Conceição, Rachel Inamassu Faccini, Guilherme Sembenelli, Gabriel Luiz Montanhim, Mareliza Possa de Menezes, Cléber Kazuo Ido, Luiz Paulo Nogueira Aires, Gabriel João Unger Carra, Paola Castro Moraes","doi":"10.1590/acb396324","DOIUrl":"10.1590/acb396324","url":null,"abstract":"Purpose: Laparoscopic cholecystectomy (LC) is the gold standard for the treatment of gallbladder (GB) disease in small animals. The aims of this study were to investigate and compare the effect of different types of dissectors during LC in rabbits; electrothermal bipolar vessel sealing device (EBVS-LigaSure) and standard electrosurgical dissection (bipolar Maryland) for dissection of the GB in LC, correlating liver function tests (LFTs) in pre and postoperative periods (days 0, 3, 7, 15); macroscopic checking 15 days after surgery through necropsy; histopathological, bacteriological through bacterial growth by culture and intraoperative complications.Methods: Twenty rabbits were used, group (n = 10) using EBVS for GB dissection and cystic duct seal (GLL), and group (n = 10) using bipolar dissecting forceps and EVBS for cystic duct seal (GLE).Results: A higher concentration of alkaline phosphatase was observed on GLL 15 days after surgery when compared to GLE. In addition, GLE resulted in a higher concentration of alanine aminotransferase at three days when compared to GLL.Conclusion: In LC no significant statistical differences were found between EBVS and bipolar Maryland; both devices are equally safe and effective in LC. Further studies are required to evaluate the effectiveness of these devices in animals with gallbladder pathologies. Therefore, clinical studies are necessary.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e396324"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin associated with bacterial cellulose-based hydrogel improves healing of skin wounds in diabetic rats. 褪黑素与细菌纤维素水凝胶可改善糖尿病大鼠皮肤伤口的愈合。

Acta cirurgica brasileira Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.1590/acb399024

Jaiurte Gomes Martins da Silva, Ismaela Maria Ferreira de Melo, Érique Ricardo Alves, Glícia Maria de Oliveira, Anderson Arnaldo da Silva, Flávia Cristina Morone Pinto, José Lamartine de Andrade Aguiar, Diego Neves Araújo, Valéria Wanderley Teixeira, Álvaro Aguiar Coelho Teixeira

{"title":"Melatonin associated with bacterial cellulose-based hydrogel improves healing of skin wounds in diabetic rats.","authors":"Jaiurte Gomes Martins da Silva, Ismaela Maria Ferreira de Melo, Érique Ricardo Alves, Glícia Maria de Oliveira, Anderson Arnaldo da Silva, Flávia Cristina Morone Pinto, José Lamartine de Andrade Aguiar, Diego Neves Araújo, Valéria Wanderley Teixeira, Álvaro Aguiar Coelho Teixeira","doi":"10.1590/acb399024","DOIUrl":"10.1590/acb399024","url":null,"abstract":"Purpose: To describe the effects of melatonin associated with bacterial cellulose-based hydrogel on healing of skin wounds in diabetic rats.Methods: Streptozotocin was used to induce diabetes in Wistar rats. After wound induction, animals were randomly divided into groups GC, GDCC, GDCB, and GDMCB. Animals were evaluated in days 3, 7, and 14 for the following variables: glycemic levels, histopathological and histochemical analyses, healing rate, morphometry and C-reactive protein.Results: There was no change in glycemic levels in the diabetic animals as a result of the treatments; histopathological analyses showed better healing in GDCB and GDMCB groups, as well as histochemistry; at day 14, the highest healing rate was observed in animals from the GDMCB group, reaching almost 100%; morphometry revealed a significant increase of fibroblasts and reduction of macrophages and blood vessels in lesions treated with bacterial cellulose associated or not with melatonin when compared to the other experimental groups. There was also an increase in C-reactive protein in GDCC group at day 14.Conclusion: Bacterial cellulose-based dressings associated with systemic melatonin showed beneficial results in experimentally induced wounds in diabetic rats, favoring the healing process.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e399024"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pretreatment of artesunate promoted hepatocyte proliferation by activating the PI3K/Akt/mTOR signaling pathway in mice. 青蒿琥酯预处理通过激活 PI3K/Akt/mTOR 信号通路促进小鼠肝细胞增殖。

Acta cirurgica brasileira Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.1590/acb394324

Changyou Lu, Xinkai Li, Chao Fang, Chuntao Li, Yunke Xu, Yong Guo

{"title":"Pretreatment of artesunate promoted hepatocyte proliferation by activating the PI3K/Akt/mTOR signaling pathway in mice.","authors":"Changyou Lu, Xinkai Li, Chao Fang, Chuntao Li, Yunke Xu, Yong Guo","doi":"10.1590/acb394324","DOIUrl":"10.1590/acb394324","url":null,"abstract":"Purpose: Artesunate (ART) has been implicated in regulating the many processes of liver injury, but its roles in liver regeneration still need to be illustrated.Methods: In the present study, ART was used to pretreat hepatocyte cell line NCTC1469 to study the effect of ART on hepatocyte proliferation in vitro. Furthermore, the potency of ART as a regimen to promote liver regeneration and restore liver function was evaluated following partial hepatectomy (PH) on C57BL/6 mice.Results: ART concentration-dependently promoted hepatocyte proliferation and reduced apoptosis. Cell cycle and Ki-67 immunohistochemical analyses demonstrated that ART supplementation promoted the proliferation of hepatocytes and accelerated liver regeneration. Our results provided evidence that ART improved liver function in a dose-dependent manner, as indicated by decreased serum alanine aminotransferase, aspartate aminotransferase, and increased albumin, and hepatocyte growth factor levels in PH mice. Meanwhile, ART promoted the PI3K/Akt/mTOR signaling in NCTC1469 cells and liver tissue of PH mice. In addition, PI3K inhibitor LY294002 blocked the promotion effect of ART on hepatocyte proliferation and cell cycle progression.Conclusion: ART promoted hepatocyte proliferation via activation of the PI3K/Akt/mTOR pathway, which was beneficial to liver regeneration of PH-induced liver injury.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"39 ","pages":"e394324"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11506702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0