{"title":"Berberine protects against lung injury induced by liver transplantation through upregulating PPARγ and suppressing NF-κB-mediated pyroptosis pathway.","authors":"Wenna Liu, Xiaohui Liang, Mingxia Huo, Yongwang Wang, Guanghua Zhang","doi":"10.1590/acb404925","DOIUrl":"10.1590/acb404925","url":null,"abstract":"<p><strong>Purpose: </strong>We builted a orthotopic autologous liver transplantation (OALT) model in rats to evaluate the possible mechanisms of berberine against lung injury.</p><p><strong>Methods: </strong>Forty clean grade Sprague-Dawley rats (male, healthy, 250-280 g) were divided into five groups (n = 8): sham-operated group (group S), orthotopic autologous liver transplantation group (group T), berberine group (group B), peroxisome proliferator-activated receptor gamma (PPARγ) inhibitor GW9662 group (group G), and berberine + GW9662 group (group B+G). In group S, the relevant tissues around the liver were dissociated only. Orthotopic autologous liver transplantation was used in other groups, berberine 200 mg/kg/day was given one week before surgery in group B and group B+G. GW9662 1 mg/kg was intraperitoneally injected in group G and group B+G 4 hours before surgery. Blood samples were obtained for detecting PaO2 and the concentration of serum clam cell protein (CC16), surfactant protein-D (SP-D), interleukin (IL)-1β and IL-18. The immunohistochemical method detects the expression of PPARγ and nuclear factor-kappa B (NF-κB) in lung tissues. The expression of PPARγ, NF-κBand pyroptosis-related proteins were analysed by western blotting.</p><p><strong>Results: </strong>Rats exhibited increased histological lung injury following OALT. Liver transplantation caused upregulated CC16, SP-D, IL-18 and IL-1β levels, reduced PaO2 and the PPARγ expression, upregulated the NF-κB and pyroptosis-related protein expressions. BBR pretreatment greatly alleviates these lung damages induced by OALT. However, administration of GW9662 partially reversed the beneficial effects of BBR on lung injury.</p><p><strong>Conclusion: </strong>Berberine may play protective capacities against lung injury by upregulating PPARγ to downregulate the NF-κB-mediated pyroptosis pathway.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e404925"},"PeriodicalIF":1.3,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Victor Pedro, Maria do Socorro Medeiros Amarante, Everlândja Gomes de Almeida, Manoel André de Souza Neto, Silvana Maria Zucolotto, Matheus Augusto de Bittencourt Pasquali, Sérgio Adriane Bezerra de Moura, Fabiane Ferreira Martins, Christina da Silva Camillo
{"title":"Antiulcerative and antioxidant action of hydroalcoholic extract of Anacardium occidentale L. leaves in an induced experimental colitis model.","authors":"Victor Pedro, Maria do Socorro Medeiros Amarante, Everlândja Gomes de Almeida, Manoel André de Souza Neto, Silvana Maria Zucolotto, Matheus Augusto de Bittencourt Pasquali, Sérgio Adriane Bezerra de Moura, Fabiane Ferreira Martins, Christina da Silva Camillo","doi":"10.1590/acb406025","DOIUrl":"10.1590/acb406025","url":null,"abstract":"<p><strong>Purpose: </strong>To examine the antiulcerative and antioxidant action of the Anacardium occidentale hydroalcoholic leaf extract (HEA) on experimentally induced colitis in rats via acetic acid (AA).</p><p><strong>Methods: </strong>Male rats were distributed into six groups (n = 10 per group): C (control), CC (colitis control), SZC (sulfasalazine 500 mg/kg), and three doses of HEA (HEA50 = 50 mg/kg, HEA100 = 100 mg/kg, HEA200 = 200 mg/kg).</p><p><strong>Results: </strong>The treatment with HEA100 for seven days decreased diarrhea, increased food intake, attenuated weight loss, and recovered the macroscopic and histological parameters of the colon, mitigating the severity of colitis and restoring the intestinal morphophysiology of animals with induced colitis. Additionally, HEA50 and HEA100 treatment increased the activity of superoxide dismutase, catalase, and thiol, and reduced the levels of thiobarbituric acid reactive substances and carbonyl, contributing to the re-establishment of antioxidant homeostasis.</p><p><strong>Conclusion: </strong>This study provided preclinical evidence of the potential of A. occidentale leaf extract, particularly at the concentration of 100 mg/kg, as an antiulcer agent for attenuating colitis in rats, likely due to the positive modulation of the antioxidant system.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e406025"},"PeriodicalIF":1.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thiago de Almeida Furtado, Álvaro Cota Carvalho, Marcelo Lopes Furtado, Luiz Ronaldo Alberti, Diego Paim de Carvalho Garcia
{"title":"Biomechanical evaluation of prophylactic reinforcement of the linea alba with a novel method of positioning and fixation of polypropylene mesh, in an animal model.","authors":"Thiago de Almeida Furtado, Álvaro Cota Carvalho, Marcelo Lopes Furtado, Luiz Ronaldo Alberti, Diego Paim de Carvalho Garcia","doi":"10.1590/acb405325","DOIUrl":"10.1590/acb405325","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effectiveness of a new format of prophylactic mesh, with a new method of placement.</p><p><strong>Methods: </strong>Rabbits were randomly distributed into four groups: control rabbits without laparotomy group; sutured rabbits (SR); light-weight mesh rabbits (LWMR); and heavy-weight mesh rabbits (HWMR). The meshes were cut to a size of 10 cm in length by 1 cm in width. A longitudinal, median abdominal incision, 11 cm in length, was performed, followed by an incision over the linea alba, encompassing the entire thickness of the abdominal wall. On the 91st postoperative day, the animals were killed for linea alba scar evaluation.</p><p><strong>Results: </strong>There was a significant difference among the evaluated groups concerning elongation, maximum load, and elasticity modulus. HWMR and LWMR were statistically different from controls regarding elongation. As for the elasticity modulus, SR presented a higher mean when compared to the others. Microscopic visual analysis showed greater fibrotic scarring with a larger amount of implanted material in HWMR.</p><p><strong>Conclusion: </strong>The reinforcement of scars with polypropylene mesh does not increase tensile strength when compared to the use of sutures. Mesh implantation results in scar remodeling with greater fibrosis, leading to increased elongation. However, midline reinforcement using only sutures has greater elasticity than the groups reinforced with mesh.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e405325"},"PeriodicalIF":1.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Berkin Gunar, Ejder Saylav Bora, Osman Mert Topkar, Özgür Baysal, Ziya Shammadli, Oytun Erbas
{"title":"Peripheral nerve injury recovery enhanced by ceftriaxone: a preclinical investigation.","authors":"Berkin Gunar, Ejder Saylav Bora, Osman Mert Topkar, Özgür Baysal, Ziya Shammadli, Oytun Erbas","doi":"10.1590/acb404625","DOIUrl":"10.1590/acb404625","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate ceftriaxone's potential in enhancing peripheral nerve injuries (PNIs) recovery.</p><p><strong>Methods: </strong>Thirty male Wistar rats were divided into control (no surgery), vehicle (sciatic nerve injury + 0.9% NaCl), and ceftriaxone (sciatic nerve injury + 50 mg/kg/day ceftriaxone) groups. Treatments were administered intraperitoneally for 12 weeks. Functional recovery was assessed using inclined plane tests and electromyography. Sciatic nerve regeneration was evaluated via histology, nerve growth factor (NGF) immunohistochemistry, and enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>Compared to the control group, inclined plane test scores, nerve action potentials, NGF expression, axon counts, and diameters were diminished in both injury groups (p 0.001). However, these parameters were significantly improved in the ceftriaxone group compared to the vehicle group (p 0.001). Increased fibrosis was observed in the ceftriaxone group.</p><p><strong>Conclusion: </strong>Ceftriaxone demonstrates potential as a pharmacological agent for PNI recovery by enhancing nerve regeneration and functional outcomes. Further studies are warranted to elucidate its mechanisms.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e404625"},"PeriodicalIF":1.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of double filtration plasmapheresis to cynomolgus monkeys: surgical techniques and a pilot study of pig fetal kidney transplantation.","authors":"Genki Watanabe, Maiko Horikawa, Izumi Yamamoto, Tomohisa Endo, Takashi Watanabe, Akinori Hiratsuka, Tsuyoshi Takamura, Kenji Matsui, Naoto Matsumoto, Yatsumu Saito, Hiroshi Sasaki, Akihiko Kiyoshi, Takao Kuroda, Makoto Inoue, Eiji Kobayashi, Takashi Yokoo","doi":"10.1590/acb405125","DOIUrl":"10.1590/acb405125","url":null,"abstract":"<p><strong>Purpose: </strong>To detail a technique to implant a double-lumen catheter to remove anti-pig antibodies. We transplanted fetal pig kidneys into cynomolgus monkeys using a double filtration plasma exchange (DFPP) protocol.</p><p><strong>Methods: </strong>Two approaches for double-lumen catheter insertion in monkeys (3-9.2 kg) were developed. DFPP was performed using hydroxyethyl starch (6%) as a replacement fluid. We transplanted fetal porcine kidneys, administered immunosuppressive agents, and evaluated the grafts.</p><p><strong>Results: </strong>The catheter insertion site was large, with postoperative hemostasis similar to blind subcutaneous puncture. Monkeys tolerated DFPP well, maintaining stable blood pressure. The technique reduced anti-pig antibodies by 67%, though acute rejection was not fully suppressed.</p><p><strong>Conclusion: </strong>A safe technique for double-lumen catheter placement in cynomolgus monkeys was developed, along with a DFPP protocol for reducing anti-pig antibodies.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e405125"},"PeriodicalIF":1.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yury Tavares de Lima, Larissa Holanda Gomes, Luiza Matias Marques, Igor Castelo Branco Fontenele Costa, Pedro Henrique Araújo Marques, Josue Viana Castro
{"title":"Pericardiocentesis simulation model: conception and development.","authors":"Yury Tavares de Lima, Larissa Holanda Gomes, Luiza Matias Marques, Igor Castelo Branco Fontenele Costa, Pedro Henrique Araújo Marques, Josue Viana Castro","doi":"10.1590/acb404725","DOIUrl":"10.1590/acb404725","url":null,"abstract":"<p><strong>Purpose: </strong>To develop a pericardiocentesis (PCT) simulation model applied for undergraduate medical training.</p><p><strong>Methods: </strong>A PCT simulator consisted of a torso mannequin, a silicone rubber heart (SRH), a container, and a filling system. The mannequin was submitted to a coronal section and an 18 × 18-cm precordial area opening. The SRH was prepared in accordance with structural dimensions of a normal heart. The elaboration of a structure container to simulate the pericardial cavity consisted of a non-leakable unit inside a cardboard (PcavBox). The PcavBox filling system was connected to a 10-mm diameter tube and a total of 2.5 L of saline solution. This structure was adapted inside the mannequin and covered with a thermoformable rubber material of 2 mm in skin color. For PCT simulation, we used a 10-mL syringe connected to a 14G needle for an imaging guided puncture facilitated by a portable ultrasound.</p><p><strong>Results: </strong>A SRH was successfully developed and fixed inside the Pcav Box, connected to the fluid pressure system. It was able to simulate a cardiac tamponade scenario identified by ultrasound. A series of 50 punctures was successively performed without liquid leak.</p><p><strong>Conclusion: </strong>A low-cost PCT simulator was developed and coan be applied to healthcare education.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e404725"},"PeriodicalIF":1.3,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pedro Afonso Kono, Rivair Gonçalves Junior, Adriana Yuriko Koga, Melquesedeque Dos Santos, Matheus Von Jelita Salina, Elder Dalazoana Filho, Alceu Toledo Junior, Leandro Cavalcante Lipinski, Marcos Ricardo da Silva Rodrigues, Eurico Cleto Ribeiro de Campos
{"title":"Effects of curcumin supplementation on the severity of intra-abdominal adhesions in rats.","authors":"Pedro Afonso Kono, Rivair Gonçalves Junior, Adriana Yuriko Koga, Melquesedeque Dos Santos, Matheus Von Jelita Salina, Elder Dalazoana Filho, Alceu Toledo Junior, Leandro Cavalcante Lipinski, Marcos Ricardo da Silva Rodrigues, Eurico Cleto Ribeiro de Campos","doi":"10.1590/acb404425","DOIUrl":"10.1590/acb404425","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of curcumin administered by gavage, on reducing intra-abdominal adhesions and attenuating the inflammatory process, assessed by a serum marker.</p><p><strong>Methods: </strong>Forty Wistar rats were randomly divided into two groups: the curcumin group, and the control group. The curcumin group received curcumin by gavage at the dose of 200 mg/kg over the seven days preceding and the seven days following surgery. In the control group, an isovolumetric administration of 0.9% saline solution was given by gavage. Both groups underwent a median laparotomy and left-sided colotomy. On the eighth postoperative day, the animals were euthanized for intracavitary adhesion analysis and left colon resection for histological examination. Intra-abdominal adhesions were classified from grade 0 to 4 based on increases in number, intensity, and ease of lysis.</p><p><strong>Results: </strong>Curcumin administration did not significantly reduce the severity of intra-abdominal adhesions (p = 0.7143) nor the severity of colonic inflammatory infiltration. However, a significant reduction in C-reactive protein levels was observed preoperatively in the curcumin group (p 0.05).</p><p><strong>Conclusion: </strong>While curcumin was not able to reduce the severity of intra-abdominal adhesions, it demonstrated the ability to attenuate the inflammatory process associated with the surgical procedure.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e404425"},"PeriodicalIF":0.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12248264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mauricio Mitsuru Yoshida, André Luiz Pires de Freitas, José da Conceição Carvalho Júnior, Julio Sergio de Souza, Vinicius Santos Baptista, Lydia Masako Ferreira
{"title":"Holographic model of craniosynostosis for HoloLens.","authors":"Mauricio Mitsuru Yoshida, André Luiz Pires de Freitas, José da Conceição Carvalho Júnior, Julio Sergio de Souza, Vinicius Santos Baptista, Lydia Masako Ferreira","doi":"10.1590/acb404825","DOIUrl":"10.1590/acb404825","url":null,"abstract":"<p><strong>Purpose: </strong>To develop a holographic skull model of a deformity resulting from craniosynostosis for the HoloLens.</p><p><strong>Methods: </strong>The methodology for product creation and prototyping was the design thinking structured with the double diamond. A desk survey was conducted with a literature review and an anteriority search. Based on the desk survey results, brainstorming was performed to develop solutions to improve the surgeon's performance in craniosynostosis using mixed reality.</p><p><strong>Results: </strong>Reports or scientific articles relating mixed reality use to craniosynostosis were not found in search engines or bibliography databases. A surgeon's performance potential improvement was observed using mixed reality as an auxiliary tool in craniosynostosis surgery. A craniosynostosis skull hologram was developed in mixed reality, with interactivity commands controlled by gestures, facilitating a three-dimensional spatial understanding of cranial anatomy.</p><p><strong>Conclusion: </strong>A holographic skull model with a deformity resulting from craniosynostosis was developed for the HoloLens.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e404825"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12233888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diego Adão, Wagner Gomes da Nóbrega Silva, Parisina Fraga Carvalho, Gabriela Caetano Lopes Martins, Georges Badin Hofmeister, Karin Romano Posegger, Adriano Meyer Pflug, Carlos Augusto Metidieri Menegozzo
{"title":"Podcast-based learning: report of podcast as an education tool for residents in the emergency room.","authors":"Diego Adão, Wagner Gomes da Nóbrega Silva, Parisina Fraga Carvalho, Gabriela Caetano Lopes Martins, Georges Badin Hofmeister, Karin Romano Posegger, Adriano Meyer Pflug, Carlos Augusto Metidieri Menegozzo","doi":"10.1590/acb404525","DOIUrl":"10.1590/acb404525","url":null,"abstract":"<p><strong>Purpose: </strong>To describe the academic utilization of podcasts as an educational tool for general surgery and emergency medicine residents.</p><p><strong>Methods: </strong>This narrative report details a one-year group experience incorporating podcasts into the curriculum of the general surgery and emergency medicine residency programs, focusing on podcast-based learning within the academic emergency department of a quaternary university hospital in an urban center in São Paulo, Brazil. The authors, comprising podcast developers, preceptors, and residents, shared their experiences with implementing and utilizing the podcast in the residency curriculum.</p><p><strong>Results: </strong>Over the course of one year, 50 episodes of the podcast \"Mania de Cirurgia\" were delivered to residents rotating in the emergency room. The episode content consisted of 64% clinical topics, 12% summaries of scientific events, 12% interviews with specialists, and 12% soft skills development. The authors reported that both residents and preceptors expressed high satisfaction with the podcast's integration as a consistent educational tool in the curriculum.</p><p><strong>Conclusion: </strong>The incorporation of a podcast as an educational tool was enthusiastically received by both residents and preceptors. Podcasts may serve as a valuable complement to traditional teaching methods, enabling updates on general surgery topics, fostering evidence-based practice, and enriching professional experiences.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e404525"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12233886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Victor Matheus Mendonça de Araújo, Manuela Rodrigues Neiva Fernandes, Lívia Guerreiro de Barros Bentes, Rafael Silva Lemos, Herick Pampolha Huet de Bacelar, Luis Otávio Amaral Duarte Pinto
{"title":"\"Gotta have balls\": bovine testicles and video magnification imaging in the varicocelectomy training.","authors":"Victor Matheus Mendonça de Araújo, Manuela Rodrigues Neiva Fernandes, Lívia Guerreiro de Barros Bentes, Rafael Silva Lemos, Herick Pampolha Huet de Bacelar, Luis Otávio Amaral Duarte Pinto","doi":"10.1590/acb405225","DOIUrl":"10.1590/acb405225","url":null,"abstract":"<p><strong>Purpose: </strong>To describe the varicocelectomy model using bull testicles and to evaluate microsurgical practice using the surgical microscope and video magnification system.</p><p><strong>Methods: </strong>Bovine testicles and spermatic cords were used, with the medial portion of the cord left free for the microsurgical varicocelectomy technique. Twenty 3rd-year medical students were divided into two groups, the video magnification system group (VSMG) and the surgical microscope group (SMG), to simulate varicocelectomy in the proposed model. Five training sessions were carried out for both groups (D1, D7, D14, D21, and D28), as well as a reassessment (D63), with a checklist applied on the first, third, and fifth day of training and on reassessment.</p><p><strong>Results: </strong>The model provides practical support for training in instrument handling, dissection of structures, anatomical identification, and vessel ligation as an alternative to learning the surgical technique. There was a drop in training time as the weeks went by, with no significant difference between the groups. There was no statistical difference in time (D14, D28, D63) or scores between groups.</p><p><strong>Conclusion: </strong>Microsurgical varicocelectomy training using testicles and sperm cords of bovine origin in the video magnification system and surgical microscope contributed to the acquisition of skills.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e405225"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12233887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}