Acta cirurgica brasileira最新文献

{"title":"The effect of liraglutide, a GLP-1 analog, on indomethacin-induced gastric ulcers in diabetic rats.","authors":"Huseyin Emre Arslan, Yasemin Teksen, Orhan Ozatik, Mustafa Cem Algin","doi":"10.1590/acb407325","DOIUrl":"10.1590/acb407325","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the potential pleiotropic effects of liraglutide (LG), a glucagon-like-peptide-1 analog, on gastric ulcer prevention in rats with diabetes induced by streptozotocin (STZ).</p><p><strong>Methods: </strong>We randomly divided 63 male Wistar rats into seven groups. STZ was administered intraperitoneally (IP) to the animals in the diabetic control (group STZ), diabetic control + indomethacin (INDO) (group STZI), STZ + INDO + omeprazole (group OMP), STZ + INDO + LG (0.2 mg/kg) (group 0.2LG), and STZ + INDO + LG (0.4 mg/kg) group (group 0.4LG). We administered OMP IP to group OMP, 0.2 mg/kg LG to group 0.2LG SC, 0.4 mg/kg LG to group 0.4LG SC, normal saline to non-diabetic control (sham group), group STZ, non-diabetic control + INDO (group KI), and group STZI SC. INDO was administered to the animals in groups KI, STZI, OMP, 0.2LG, and 0.4LG by gavage. Then, the caspase-3, epidermal growth factor (EGF), vascular endothelial growth factor-A (VEGF-A), prostaglandin E2 (PGE2), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), superoxide dismutase-1 (SOD-1), glutathione (GSH), and malondialdehyde (MDA) levels were studied.</p><p><strong>Results: </strong>LG prevented INDO-induced ulcers and decreased apoptosis in the stomach tissue. It increased the SOD-1, GSH, EGF, VEGF-A, and PGE2 levels, and reduced the MDA, IL-6, and TNF-α levels. The anti-ulcer effect of LG was lower, but close to that of OMP.</p><p><strong>Conclusion: </strong>The antioxidant, anti-inflammatory, and anti-apoptotic effects of LG, its ability to regulate EGF, VEGF-A, and PGE2 levels, and its capacity to reduce blood glucose levels in diabetic rats may contribute to its anti-ulcer effect.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e407325"},"PeriodicalIF":1.3,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematemesis is associated with worse outcomes in upper gastrointestinal bleeding: a retrospective study.","authors":"Gabriela Caetano Lopes Martins, Carolina Villalba Moya Rodrigues, Lucas Domingos Ribeiro, Karin Romano Posegger, Rafael Leite Pacheco, Leonardo de Mello Del Grande, Diego Adão","doi":"10.1590/acb407125","DOIUrl":"10.1590/acb407125","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate whether hematemesis is associated with increased morbidity and mortality for upper gastrointestinal bleeding.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted at a quaternary university hospital from January 2022 to September 2024. Adults presenting with upper gastrointestinal bleeding, confirmed by endoscopy, were included. We excluded patients with terminal disease, patients who refused to receive blood products, and trauma. The main outcomes were all-cause mortality, need for orotracheal intubation, emergency blood transfusion, need for re-endoscopy, and length of hospital and intensive care unit (ICU) stays.</p><p><strong>Results: </strong>A total of 69 patients (65% male, mean age 58 years) were included. Hematemesis was associated with a higher need for emergency blood transfusions (73% vs. 23%; odds ratio - OR = 8.82, 95% confidence interval - 95%CI 2.44-31.94, p = 0.001), longer hospital (12 vs. 6 days; mean difference - MD = 6.02, 95%CI 2.39-9.64, p = 0.001) and ICU stays (7.7 vs. 3.2 days; MD = 4.5, 95%CI 1.73-7.26, p = 0.002). Data were sparse and imprecise on all-cause mortality, orotracheal intubation, and the need for re-endoscopy.</p><p><strong>Conclusion: </strong>Hematemesis is associated with higher transfusion requirements and longer hospital and ICU stays. These findings highlight the potential predictive value of hematemesis in acute upper gastrointestinal bleeding.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e407125"},"PeriodicalIF":1.3,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of enoxaparin treatment against uterus ischemia/reperfusion injury in rats.","authors":"Duygu Lafci, Mustafa Hilmi Yaranoglu, Eren Altun, Eray Metin Guler, Ceyda Sancakli Usta","doi":"10.1590/acb407225","DOIUrl":"10.1590/acb407225","url":null,"abstract":"<p><strong>Purpose: </strong>This study analyzed the protective effects of enoxaparin in rat uteruses by exposing the uterus to experimental ischemia-reperfusion injury.</p><p><strong>Methods: </strong>Thirty female rats were homogenized by weight and cycle and divided into three groups: a control group, an ischemia-reperfusion (I/R) group, and an ischemia-reperfusion plus enoxaparin (I/R+E) group. An experimental uterine I/R model was established in the I/R and I/R+E groups. Unlike I/R group, all rats in the I/R+E group received subcutaneous enoxaparin at 0.5 mg/kg 2 hours before ischemia. In histopathological analysis, endometrial glandular and endo/myometrial stromal changes were scored according to the histopathological scoring system. Biochemically, catalase (CAT), superoxide dismutase (SOD) enzyme activities, and malondialdehyde (MDA) levels were measured in uterine tissues.</p><p><strong>Results: </strong>In histopathological analysis, all of the control group's scores were lower than those of the other groups, except for necrosis (p 0.05). There was no significant improvement in the glandular and stromal changes between I/R and I/R+E (p 0.05). However, the I/R+E group showed significantly increased SOD and CAT activities and decreased MDA levels compared to the I/R group (p = 0.000).</p><p><strong>Conclusion: </strong>Although enoxaparin did not significantly improve histopathological injury, its potent antioxidant effects suggest a protective role against oxidative stress in uterine I/R injury.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e407225"},"PeriodicalIF":1.3,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the protective efficacy of Caesalpinia sappan and spirulina against ethanol-induced experimental rat gastritis model.","authors":"İdris Oruç, Zelal Karakoç, Filiz Akduman, Zeynep Oruç, Nazan Baksi, Muzaffer Aydin Ketani","doi":"10.1590/acb406725","DOIUrl":"10.1590/acb406725","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the protective effect of Caesalpinia sappan and spirulina against gastritis and determine changes in the expression of CD8, CD68, and major histocompatibility complex (MHC) molecules.</p><p><strong>Methods: </strong>Gastritis was induced in 24 female Wistar albino rats on the first day using ethanol. The treatment groups were given C. sappan (250 mg/kg) and spirulina (400 mg/kg) using oral gavage for five days. Blood and stomach tissue samples of the mice were analyzed.</p><p><strong>Results: </strong>Caesalpinia sappan and spirulina increased CD8 and CD68 expression and tumor necrosis factor-alpha levels thereby decreasing the severity of inflammation. It was found that they simultaneously decreased MHC I and MHC II expressing cells, increased superoxide dismutase levels, whereas malondialdehyde, and myeloperoxidase levels decreased in the treatment group.</p><p><strong>Conclusion: </strong>This study revealed that C. sappan and spirulina can protect gastric mucosa by reducing oxidative stress and inflammation.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e406725"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of fisetin on ovarian ischemia-reperfusion injury in rats via modulation of the TLR4-MyD88-TRAF6 signaling pathway.","authors":"Qianqian Gao, Dan Zhao, Wencong He","doi":"10.1590/acb405925","DOIUrl":"10.1590/acb405925","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the protective effects of fisetin on ovarian ischemia-reperfusion injury in rats, focusing on the modulation of the TLR4-MyD88-TRAF6 signaling pathway.</p><p><strong>Methods: </strong>Wistar rats randomly divided into different groups received oral administration of fisetin (5, 10 and 15 mg/kg). Body weight and ovary weight were measured. Hormones, hematological, antioxidant, cytokines, inflammatory and apoptosis parameters were assessed. Histological and histopathologic evaluations were conducted on ovary tissue. Different mRNA expressions were estimated.</p><p><strong>Results: </strong>Dose dependent treatment significantly improved body and ovary weight along with alteration in hematological parameters such as red blood cells, white blood cells, hemoglobin, platelet, lymphocyte; antioxidant parameters, including malonaldehyde, superoxide dismutase, glutathione, glutathione peroxidase, catalase; cytokines viz., tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-18 (IL-18); inflammatory parameters such as cyclooxynase-2, inducible nitric oxide synthetase (iNOS), prostaglaindin, nuclear kappa B factor (NF-κB), C reactive protein and apoptosis parameters, including Bcl-2, Bax, and caspase-3. Fisetin treatment significantly (p < 0.001) altered the mRNA expression of TNF-α, IL-6, IL-1β, IL-10, Bax, Bcl-2, caspase-3, toll-like receptor 4, myeloid differentiation primary response protein 88, tumor necrosis factor receptor-associated factor 6, endothelial nitric oxide synthase, iNOS, NF-κB, inhibitor of kappaB kinase alpha, heme oxygenase-1, and nuclear factor erythroid 2-related factor 2. Fisetin treatment altered the hemorrhage, vascular proliferation, polymorphonuclear leukocyte, edema, vascular congestion, and apoptosis.</p><p><strong>Conclusion: </strong>Fisetin ameliorated the ovary injury against ovarian ischemia-reperfusion via conquering TLR4-MyD88-TRAF6 signaling pathway.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e405925"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of AMPK/OPA1 pathway alleviates traumatic brain damage by regulating mitophagy.","authors":"Hao Wei, Jiushan Liao, Wei Gao, Xiangzhong He","doi":"10.1590/acb406925","DOIUrl":"10.1590/acb406925","url":null,"abstract":"<p><strong>Purpose: </strong>Mitophagy is an important process in brain damage, and the precise impact on a traumatic brain injury (TBI) model remains unclear. AMP-activated protein kinase (AMPK) regulates mitochondrial homeostasis and mitophagy, which are closely related to the remission of early brain injury. This study sought to explore the mechanism behind AMPK/optic atrophy 1 (OPA1) pathway in TBI via experimental verifications.</p><p><strong>Methods: </strong>TBI mouse model induced by weight-drop method was applied in this study. Neurological function tests, Nissl staining, TUNEL staining, and transmission electron microscopy were undertaken to assess the effects of mitophagy on the TBI model. Levels of apoptosis-related factors and mitophagy-related indicators were detected to further reveal the molecular regulatory mechanism of mitophagy in TBI.</p><p><strong>Results: </strong>Activation of mitophagy (MK-8722 or rapamycin treatment) reduced the severity of brain damage and mitigated neurological function deficits following TBI. MK-8722 treatment reduced neuronal apoptosis, improved neuronal mitophagy, effectively inhibited the expression of proteins Bcl-2 and Bax, and increased the expression of proteins Parkin, PINK1 and OPA1. Besides, MK-8722 improved TBI through accelerating the AMPK/OPA1 pathway, resulting in increase of mitophagy.</p><p><strong>Conclusion: </strong>This study is the first to pinpoint the AMPK/OPA1 pathway's involvement in TBI and the mechanism of mitophagy, thereby providing a good foundation for future experimental studies.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e406925"},"PeriodicalIF":1.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective effect of lotus leaf against non-alcoholic fatty liver disease in rats via alteration of AMPK/SIRT1 and Nrf2/HO-1 signaling pathway.","authors":"Qingxia Shen, Junqian Wang, Na Yao, Xiyan Niu, Mi Liu, Xiaohui Li","doi":"10.1590/acb407025","DOIUrl":"10.1590/acb407025","url":null,"abstract":"<p><strong>Purpose: </strong>In this study, we scrutinized the protective effect of lotus leaf (LF) against high-fat diet (HFD) induced liver injury in rats.</p><p><strong>Methods: </strong>The rats received the HFD for the induction of non-alcoholic fatty liver disease. Rats received the oral administration of LF (25, 50, and 100 mg/kg, b.w.). The insulin level, organ index, glucose level, hepatic, oxidative stress, lipid and cytokines parameters were measured. The different mRNA expression and histopathology were performed in the hepatic tissue.</p><p><strong>Results: </strong>LF treatment suppressed the insulin, glucose and HOMA-IR along with organ index (liver index and spleen index). LF treatment altered the level of liver parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase) and oxidative stress parameters in the serum, as well as the liver tissue. LF treatment altered the level of lipid parameters and fat parameters (total fat, perirenal fat, abdominal fat, epididymal fat); cytokines (tumor necrosis factor-α, interleukin-1β, interleukin-6, interleukin-10, interleukin-17, interleukin-33); HO-1, and Nrf2. LF treatment altered the mRNA expression of tumor necrosis factor-α, interleukin-1β, interleukin-6, interleukin-10, caspase-3, caspase-9, cytochrome C, cytochrome D, AMP-activated protein kinase (AMPK), sirtuin 1 (SIRT1), FRX-1, liver X Receptor alpha, fibronectin, matrix metalloproteinase-9, inducible nitric oxide synthase, and transforming growth factor-β 1 (TGF-β1). LF treatment suppressed the necrosis of hepatocytes with less inflammatory cell infiltration in the liver tissue along with alteration of liver injury score.</p><p><strong>Conclusion: </strong>The result showed the protective effect of LF against non-alcoholic fatty liver disease via activating the AMPK/SIRT1 and Nrf2/HO-1 pathway activation.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e407025"},"PeriodicalIF":1.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Randomized clinical trial comparing removal followed by topical imiquimod versus removal followed by topical methylprednisolone in the treatment of keloids.","authors":"Alexandre Spiandorello Ricciardi, Marcio Fernandes Chedid, Claudia Elizabeth Thompson, Rafaela Katrine da Silva, David Rubem Azulay, Mônica Manela-Azulay","doi":"10.1590/acb406225","DOIUrl":"10.1590/acb406225","url":null,"abstract":"<p><strong>Purpose: </strong>Keloids are unaesthetic benign dermatosis characterized by a disorganized proliferation of collagen. Treatment of keloids constitutes a therapeutic challenge. The aim of this study was to evaluate the efficacy and effectiveness of topical imiquimod associated with surgical excision in the treatment of keloid.</p><p><strong>Methods: </strong>A randomized, double blind, matching-lesion (self-paired manner) clinical trial. Ten patients with two keloid lesions each in similar anatomical and contralateral areas (paired lesions) had their keloids excised, and the operative site treated with the application of 5% imiquimod cream or 0.1% methylprednisolone aceponate cream (gold standard) for eight weeks.</p><p><strong>Results: </strong>Eight patients (total = 16 lesions) completed the study. Four of the total eight keloids (50%) in the methylprednisolone group vs. 3/8 keloids (37.5%) in the imiquimod group recurred in the first post-treatment year (p 0.05).</p><p><strong>Conclusion: </strong>Surgical removal plus application of topical imiquimod was shown as safe, and its efficacy was not statistically inferior for the treatment of keloids as compared to methylprednisolone. Due to the lack of efficacy in most therapeutic modalities, surgical removal plus topical imiquimod could be recommended as an additional first line therapy and especially for recurrent keloids. Studies with larger samples are necessary to evaluatre therapies for keloids.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e406225"},"PeriodicalIF":1.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frog skin as an alternative temporary dressing for diabetic foot ulcers treatment: animal model.","authors":"Cristina Pires Camargo, Janayna Terlera Paulino, Deborah Luisa de Sousa Santos, Bianca Loula Santos, Ezequiel da Silva Passos Porto, Maria José Ferreira Alves, Miyuki Uno, Tatiane Katsue Furuya, Rolf Gemperli","doi":"10.1590/acb406625","DOIUrl":"https://doi.org/10.1590/acb406625","url":null,"abstract":"<p><strong>Purpose: </strong>Diabetic foot ulcers (DFUs) are chronic lesions, and despite extensive research, no standardized treatment exists yet. This study aimed to evaluate the effect of frog skin application as a temporary dressing on the DFUs of diabetic rats.</p><p><strong>Methods: </strong>Diabetes was induced in male Wistar rats (n = 22) weighing 200-300 g through a streptozotocin injection (55 mg/kg). The animals received sub-doses of long-acting insulin to induce a moderate, chronic diabetic state. A DFU was surgically created on the animal's paws (1 × 1 cm). The animals were divided into two groups: a control group treated with gauze and 0.9% saline solution (n = 10), and the frog skin group (n = 12). Both groups were further subdivided so it was possible to analyze their healing on postoperative 7 and 14 days (POD7 and POD14). The primary endpoints assessed included DFU contraction area, histological analysis, and gene expression.</p><p><strong>Results: </strong>On POD14, the frog skin group showed six times less contracture when compared to the control group. Histological analysis revealed a 30% increase in neoangiogenesis and 50% reduction in inflammation in the POD14 frog skin group when compared to the control group. Additionally, on POD7, Il10 expression was about five times higher in the frog skin group compared to the respective control (p = 0.011).</p><p><strong>Conclusion: </strong>This study suggests that the use of frog skin as temporary biological dressing can reduce DFU secondary contraction and inflammation, offering potential therapeutic benefits for chronic wound management.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e406625"},"PeriodicalIF":1.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12360677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corynoline alleviates renal ischemia-reperfusion injury by enhancing Nrf2/HO-1 pathway.","authors":"Ming Chang, Buhe Sichen","doi":"10.1590/acb406825","DOIUrl":"https://doi.org/10.1590/acb406825","url":null,"abstract":"<p><strong>Purpose: </strong>The potential of corynoline in ameliorating oxidative stress and inflammatory responses has been extensively demonstrated across various diseases. However, its specific role in the context of renal ischemia-reperfusion (IR) injury remains elusive. The aim of this study was to investigate the role of corynoline in renal IR injury and its mechanism.</p><p><strong>Methods: </strong>A rat model of renal IR injury was successfully developed. Samples of kidney tissue and blood were obtained to evaluate alterations in tissue damage, inflammatory response, oxidative stress, and apoptosis.</p><p><strong>Results: </strong>Corynoline significantly reduced renal IR injury, thus leading to improved renal function and mitigated tissue structure damage and cell apoptosis. Moreover, corynoline effectively suppressed the oxidative stress and inflammatory response induced by IR by increasing the superoxide dismutase (SOD) content, reducing the malondialdehyde (MDA) level, inhibiting neutrophil infiltration, and suppressing the release of proinflammatory cytokines. Mechanistically, corynoline successfully restored the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), which were significantly inhibited during renal IR injury. Furthermore, when coadministered with ML-385 (an Nrf2 inhibitor), the protective effect of corynoline against renal IR injury was counteracted.</p><p><strong>Conclusion: </strong>Corynoline protects against renal IR injury by suppressing oxidative stress, inflammatory responses, and apoptosis, and its mechanism of action may involve the activation of the Nrf2/HO-1 pathway.</p>","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e406825"},"PeriodicalIF":1.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12360678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144982180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}