Application of double filtration plasmapheresis to cynomolgus monkeys: surgical techniques and a pilot study of pig fetal kidney transplantation.

IF 1.3

Acta cirurgica brasileira Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI:10.1590/acb405125

Genki Watanabe, Maiko Horikawa, Izumi Yamamoto, Tomohisa Endo, Takashi Watanabe, Akinori Hiratsuka, Tsuyoshi Takamura, Kenji Matsui, Naoto Matsumoto, Yatsumu Saito, Hiroshi Sasaki, Akihiko Kiyoshi, Takao Kuroda, Makoto Inoue, Eiji Kobayashi, Takashi Yokoo

{"title":"Application of double filtration plasmapheresis to cynomolgus monkeys: surgical techniques and a pilot study of pig fetal kidney transplantation.","authors":"Genki Watanabe, Maiko Horikawa, Izumi Yamamoto, Tomohisa Endo, Takashi Watanabe, Akinori Hiratsuka, Tsuyoshi Takamura, Kenji Matsui, Naoto Matsumoto, Yatsumu Saito, Hiroshi Sasaki, Akihiko Kiyoshi, Takao Kuroda, Makoto Inoue, Eiji Kobayashi, Takashi Yokoo","doi":"10.1590/acb405125","DOIUrl":null,"url":null,"abstract":"Purpose: To detail a technique to implant a double-lumen catheter to remove anti-pig antibodies. We transplanted fetal pig kidneys into cynomolgus monkeys using a double filtration plasma exchange (DFPP) protocol.Methods: Two approaches for double-lumen catheter insertion in monkeys (3-9.2 kg) were developed. DFPP was performed using hydroxyethyl starch (6%) as a replacement fluid. We transplanted fetal porcine kidneys, administered immunosuppressive agents, and evaluated the grafts.Results: The catheter insertion site was large, with postoperative hemostasis similar to blind subcutaneous puncture. Monkeys tolerated DFPP well, maintaining stable blood pressure. The technique reduced anti-pig antibodies by 67%, though acute rejection was not fully suppressed.Conclusion: A safe technique for double-lumen catheter placement in cynomolgus monkeys was developed, along with a DFPP protocol for reducing anti-pig antibodies.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e405125"},"PeriodicalIF":1.3000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12273875/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta cirurgica brasileira","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/acb405125","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: To detail a technique to implant a double-lumen catheter to remove anti-pig antibodies. We transplanted fetal pig kidneys into cynomolgus monkeys using a double filtration plasma exchange (DFPP) protocol.

Methods: Two approaches for double-lumen catheter insertion in monkeys (3-9.2 kg) were developed. DFPP was performed using hydroxyethyl starch (6%) as a replacement fluid. We transplanted fetal porcine kidneys, administered immunosuppressive agents, and evaluated the grafts.

Results: The catheter insertion site was large, with postoperative hemostasis similar to blind subcutaneous puncture. Monkeys tolerated DFPP well, maintaining stable blood pressure. The technique reduced anti-pig antibodies by 67%, though acute rejection was not fully suppressed.

Conclusion: A safe technique for double-lumen catheter placement in cynomolgus monkeys was developed, along with a DFPP protocol for reducing anti-pig antibodies.

Abstract Image

查看原文本刊更多论文

双滤浆分离法在食蟹猴上的应用：手术技术及猪胎肾移植的初步研究。

目的：详细介绍一种植入双腔导管去除抗猪抗体的技术。我们采用双过滤血浆交换（DFPP）方案将猪胎儿肾脏移植到食蟹猴体内。方法：采用两种方法对3 ~ 9.2 kg的猕猴进行双腔置管。DFPP采用羟乙基淀粉（6%）作为替代液。我们移植了猪胎儿肾脏，给予免疫抑制剂，并评估移植物。结果：置管部位大，术后止血效果与盲皮下穿刺相似。猴子对DFPP耐受性良好，血压保持稳定。这项技术减少了67%的抗猪抗体，尽管急性排斥反应并没有完全被抑制。结论：开发了一种安全的食蟹猴双腔导管置入技术，以及一种降低抗猪抗体的DFPP方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta cirurgica brasileira

自引率

0.00%

发文量