Acta cirurgica brasileira最新文献_第2页

Chemoprotective effect of nimbolide against N-methyl-N-nitrosourea induced gastric cancer via alteration of apoptosis and NF-κB signaling pathway. nimbolide通过改变细胞凋亡和NF-κB信号通路对n -甲基-n -亚硝基脲诱导胃癌的化学保护作用。

Acta cirurgica brasileira Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.1590/acb402125

Yizhong Gu, Binguo Liu, Xiaoting Xia, Chunlei Luo, Yi Ren

{"title":"Chemoprotective effect of nimbolide against N-methyl-N-nitrosourea induced gastric cancer via alteration of apoptosis and NF-κB signaling pathway.","authors":"Yizhong Gu, Binguo Liu, Xiaoting Xia, Chunlei Luo, Yi Ren","doi":"10.1590/acb402125","DOIUrl":"10.1590/acb402125","url":null,"abstract":"Purpose: Gastric cancer (GC) ranks as the third most common cause of cancer related mortality and as the fifth most frequently diagnosed cancer globally. Less than 30% of people with GC survive for more than five years.Methods: Nimbolide has been shown to have anticancer, anti-inflammatory, antiparasitic, and antioxidant properties. The current investigation showed the anticancer effect of nimbolide against N-methyl-N-nitrosourea (MNU) induced GC in rats. Rats were given MNU (100 mg/kg) orally to induce GC and received the oral administration of nimbolide (10, 20 and 40 mg/kg). The different biochemical parameters were estimated.Results: Nimbolide significantly (p < 0.001) altered the level of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), cytochrome P450, cytochrome B5 and histone deacetylase (HDAC) activity. Nimbolide treatment significantly (p < 0.001) altered the level of antioxidant parameters like superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), malondialdehyde (MDA); cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-2, IL-6; inflammatory parameters viz., cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), vascular endothelial growth factor (VEGF), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in the serum and stomach tissue. Nimbolide considerably altered (p < 0.001) the level of apoptosis parameters (Bcl-2, Bax and caspase-3), and the mRNA expression of VCAM-1, ICAM-1, TNF-α, IL-1β, IL-6, MCP-1, TLR4 and NF-κB.Conclusion: Nimbolide treatment considerably altered the GC against MNU induced GC via alteration of apoptosis and NF-κB signaling pathway.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402125"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11960577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the effect of thymoquinone and doxorubicin on the EGFR/FOXP3 signaling pathway in OVCAR3 human ovarian adenocarcinoma cells. 百里醌和阿霉素对OVCAR3人卵巢腺癌细胞EGFR/FOXP3信号通路影响的研究

Acta cirurgica brasileira Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.1590/acb401725

İlhan Özdemir, Ayfer Şanli Aktaş, Mehmet Cudi Tuncer

{"title":"Investigation of the effect of thymoquinone and doxorubicin on the EGFR/FOXP3 signaling pathway in OVCAR3 human ovarian adenocarcinoma cells.","authors":"İlhan Özdemir, Ayfer Şanli Aktaş, Mehmet Cudi Tuncer","doi":"10.1590/acb401725","DOIUrl":"10.1590/acb401725","url":null,"abstract":"Purpose: To investigate the cytotoxic and apoptotic effects of the combination of doxorubicin (Dox) and thymoquinone (TQ) on ovarian adenocarcinoma cells (OVCAR3) via the EGFR/FOXP3 signaling pathway.Methods: We used human OVCAR3 and human skin keratinocyte cells (HaCaT). Different concentrations of TQ and Dox were applied to the cells for 24, 48, and 72 hours, and the cytotoxicity level was determined via the MTT method. Expression levels of EGFR/FOXP3 for cell proliferation and apoptosis were determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blot analysis. The colony counting was performed after DAPI staining, and the effect on cell proliferation was determined.Results: Cytotoxicity was found to be highest with TQ and Dox treatments, and cell migration was prevented, especially in the group that received combined TQ and Dox treatment. Moreover, using RT-qPCR analysis, activity in the EGFR and FOXP3 pathway was found to be downregulated the most with TQ, and the amount of protein decreased with TQ and Dox.Conclusions: The findings showed that the greatest cytotoxic effect and the most apoptosis occurred during TQ treatment. Additionally, it was determined that a significant decrease in EGFR and FOXP3 levels occurred with the application of TQ and Dox.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e401725"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11960576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of photobiomodulation therapy in treating and preventing antineoplastic-induced oral mucositis: a systematic review. 光生物调节治疗和预防抗肿瘤性口腔黏膜炎的机制：系统综述。

Acta cirurgica brasileira Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.1590/acb403125

Paulo Victor Alves de Sales, Isabella Piassi Dias Godói, Gerly Anne de Castro Brito, Renata Carvalho Leitão, Aurigena Antunes de Araújo, Caroline Addison Carvalho Xavier de Medeiros

{"title":"Mechanisms of photobiomodulation therapy in treating and preventing antineoplastic-induced oral mucositis: a systematic review.","authors":"Paulo Victor Alves de Sales, Isabella Piassi Dias Godói, Gerly Anne de Castro Brito, Renata Carvalho Leitão, Aurigena Antunes de Araújo, Caroline Addison Carvalho Xavier de Medeiros","doi":"10.1590/acb403125","DOIUrl":"10.1590/acb403125","url":null,"abstract":"Purpose: To conduct a systematic review of the mechanisms of photobiomodulation therapy (PBMT) for treating or preventing oral mucositis (OM) caused by antineoplastic therapy.Methods: Following PRISMA 2020 guidelines, a search was conducted in Medline, Latin American and Caribbean Health Sciences Literature (LILACS), Scientific Electronic Library Online (SciELO), and Bibliografia Brasileira de Odontologia from August to September 2023 using descriptors related to OM and laser therapy. Studies on the mechanisms of photobiomodulation in OM were included. Randomized (RCTs) or non-randomized trials from the past 10 years were reviewed. Risk of bias was assessed using RoB 2.0 and ROBINS-I tools.Results: A total of 355 studies was identified. After the screening, seven met the eligibility criteria. The RCTs showed a low risk of bias. PBMT reduced OM incidence in patients undergoing chemotherapy/radiotherapy. PBMT decreased pro-inflammatory cytokines (interleukin-6, tumor necrosis factor-α) and increased anti-inflammatory cytokines (interleukin-4, interleukin-10). It also modulated inflammatory mediators, enhancing the antioxidant enzyme superoxide dismutase and overexpressing genes for keratinocyte differentiation, aiding injury repair.Conclusion: The findings suggested that the mechanism of action of PBMT in OM involves modulation of the inflammatory response, balancing oxygen reactive species generation, and expression of factors related to healing or repair. Further studies are needed to elucidate these mechanisms and optimize treatment protocols.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e403125"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11960598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastrointestinal histological injury in pigs subjected to triple stent interposition in the thoracoabdominal aorta. 猪胸腹主动脉支架置入后的胃肠道组织学损伤。

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI: 10.1590/acb402425

Angela Claudia Paixão Soares de Magalhães, Gutenberg do Amaral Gurgel, Svetlana Maria Wanderley de Barros, Miguel Lucas Silva Valente, Maurício de Amorim Aquino, Sthefanie da Silva Bessa, Rogério Ferraz Baquette, Aldemar Araújo Castro, Guilherme Benjamim Brandão Pitta

{"title":"Gastrointestinal histological injury in pigs subjected to triple stent interposition in the thoracoabdominal aorta.","authors":"Angela Claudia Paixão Soares de Magalhães, Gutenberg do Amaral Gurgel, Svetlana Maria Wanderley de Barros, Miguel Lucas Silva Valente, Maurício de Amorim Aquino, Sthefanie da Silva Bessa, Rogério Ferraz Baquette, Aldemar Araújo Castro, Guilherme Benjamim Brandão Pitta","doi":"10.1590/acb402425","DOIUrl":"10.1590/acb402425","url":null,"abstract":"Purpose: To evaluate gastrointestinal histological injury in pigs subjected to triple stent interposition versus a control group, hypothesizing no significant injury increase with triple stents.Methods: A prospective study with 15 pigs divided into a control group (G0, n = 5) undergoing arteriography only, and a triple stent group (G3, n = 10) undergoing arteriography and three stent implantations in the thoracoabdominal aorta. After an eight-day observation, arteriography, euthanasia, and en bloc gastrointestinal harvesting were performed. Lesions were graded using the Park/Chiu classification, and serum markers were analyzed pre- and post-procedure.Results: Arteriography confirmed mesenteric artery patency in all animals. Histological analysis showed ischemic lesions in 88.9% of G3, mainly in the colon (89%), compared to 60% in G0, primarily in the colon (60%) and stomach (40%). Most G3 lesions were grade 1, while G0 had higher-grade lesions. Serum markers showed no significant intergroup differences.Conclusion: Triple stent interposition did not significantly increase gastrointestinal injury, indicating its safety for maintaining gastrointestinal perfusion in this model.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402425"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of blood concentrates in controlling inflammatory signs and symptoms after lower third molar extractions: an overview. 血液浓缩物在控制下第三磨牙拔牙后炎症体征和症状中的作用：综述

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI: 10.1590/acb401825

Vinícius Lima de Almeida, Marcelo Dias Moreira de Assis Costa, Caio Melo Mesquita, Walbert Andrade Vieira, Rafael Rodrigues Lima, Livia Bonjardim Lima, Sigmar de Mello Rode, Luiz Renato Paranhos

{"title":"Performance of blood concentrates in controlling inflammatory signs and symptoms after lower third molar extractions: an overview.","authors":"Vinícius Lima de Almeida, Marcelo Dias Moreira de Assis Costa, Caio Melo Mesquita, Walbert Andrade Vieira, Rafael Rodrigues Lima, Livia Bonjardim Lima, Sigmar de Mello Rode, Luiz Renato Paranhos","doi":"10.1590/acb401825","DOIUrl":"10.1590/acb401825","url":null,"abstract":"Purpose: To summarize the available evidence and answer the following question: What is the current knowledge on the performance of blood concentrates in handling sequelae after lower third molar extractions with the evidence available in systematic reviews?Methods: An electronic search was conducted across nine databases. The study included systematic reviews with or without meta-analyses investigating the performance of blood concentrates in managing sequelae after lower third molar extractions. The four outcomes analyzed were pain, edema, mouth opening, and alveolar osteitis. The AMSTAR-2 tool assessed the methodological quality of the included systematic reviews, while ROBIS evaluated the risk of bias.Results: The electronic search revealed 690 records, of which 15 were eligible systematic reviews for the present study. Overall, these reviews evaluated 75 primary studies published from 2007 to 2023. According to AMSTAR-2, only one systematic review presented high methodological quality. The ROBIS tool showed two systematic reviews with a low risk, and the others had a high risk of bias.Conclusion: The current evidence is based on only one systematic review with high methodological quality and a low risk of bias, while the others exhibited a high risk of bias and low methodological quality. Therefore, the evidence regarding the efficacy of blood concentrates in controlling sequelae following lower third molar extractions is inconclusive.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e401825"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of toxicity, local biocompatibility, biodegradation, and systemic metabolism of cellulose/alginate/strontium apatite membranes implanted subcutaneously in mice. 小鼠皮下植入纤维素/海藻酸盐/磷灰石锶膜的毒性、局部生物相容性、生物降解和全身代谢评价。

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI: 10.1590/acb401925

Juliana Dantas da Costa, Erika Iara de Souza Araújo, Juan Feliphe Silva de Castro, Tamiris Bezerra Costa, Érika Patrícia Chagas Gomes Luz, Rodrigo Silveira Vieira, Igor Iuco Castro-Silva

{"title":"Evaluation of toxicity, local biocompatibility, biodegradation, and systemic metabolism of cellulose/alginate/strontium apatite membranes implanted subcutaneously in mice.","authors":"Juliana Dantas da Costa, Erika Iara de Souza Araújo, Juan Feliphe Silva de Castro, Tamiris Bezerra Costa, Érika Patrícia Chagas Gomes Luz, Rodrigo Silveira Vieira, Igor Iuco Castro-Silva","doi":"10.1590/acb401925","DOIUrl":"10.1590/acb401925","url":null,"abstract":"Purpose: To evaluate membranes originating from pure or oxidized bacterial cellulose (BC)/alginate/strontium apatite hydrogels regarding toxicity, biocompatibility, biodegradation and metabolism.Methods: The toxicity was measured by incubating the materials with Artemia salina for 24 h, and mortality and the 50% lethal concentration were determined in comparison to potassium dichromate by Probit analysis. Local biocompatibility and biodegradation were evaluated by subcutaneous assay in 75 Swiss mice; the test groups were compared to sham and collagen membrane at one, three and nine weeks. The histopathology of tissue irritation followed the ISO 10993-6 standard, and the integrity of the biomaterials scored by quartiles. Metabolic analysis of relative weight and the intensity of catalase, iodine and nitrite were carried out for liver, kidneys and tibias of the tested animals.Results: All cellulose-based materials were nontoxic, biocompatible, and none presented nitrosative stress. The oxidized BC was more resorbable, and the non-oxidized BC had greater renal biochemical reactivity.Conclusion: The membranes suggest applicability as regenerative barriers. However, long-term studies in bone defects are necessary to elucidate their osteopromoting efficiency.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e401925"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of low- and high-dose methotrexate on wound healing in rats. 低、高剂量甲氨蝶呤对大鼠创面愈合的影响。

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI: 10.1590/acb403225

Abdullah Karasu, Yağmur Kuşcu, Caner Kayikci, Serkan Yildirim, Oğuzhan Kuşcu, Metin Kiliçlioğlu

{"title":"Effect of low- and high-dose methotrexate on wound healing in rats.","authors":"Abdullah Karasu, Yağmur Kuşcu, Caner Kayikci, Serkan Yildirim, Oğuzhan Kuşcu, Metin Kiliçlioğlu","doi":"10.1590/acb403225","DOIUrl":"10.1590/acb403225","url":null,"abstract":"Purpose: To investigate the effect of intraperitoneal treatment with low- and high-dose methotrexate (MTX) on wound healing in rats.Methods: The study sample consisted of 54 healthy rats. Under aseptic conditions, skin wounds were created with two circular full-thickness punch tools, 10 mm in diameter, one on the right and the other one on the left of the dorsal vertebral line. The rats were randomly assigned to one of three main treatment groups. On the 0th day (2 hours before wound creation), 7th day, and 14th day, the control group received 0.3-mL saline, the low-MTX group received 3 mg/kg MTX, and the high-MTX group received 30 mg/kg MTX, all administered intraperitoneally. The wounds were evaluated seven, 14, and 21 days after injury through morphometrical, biochemical, histopathological, and immunohistochemical analyses.Results: MTX dose-dependently decreased the degree of inflammation and angiogenesis, tissue hydroxyproline level, and HSP70 and tumor necrosis factor-α expression in the early phase of wound healing. It also suppressed epithelialization and collagen 1 expression throughout the wound-healing process.Conclusion: The wounds treated with high-dose of MTX had statistically delayed wound closure on days 7, 14 and 21 compared to the saline group, while wounds treated with low-dose of MTX only had statistically delayed wound closure on day 14. In addition, weight loss was observed in rats treated with high-dose MTX, which was thought to reflect its toxicity. The dose-dependent adverse effect of MTX on wound healing may be due to its antiproliferative, antifibrotic, anti-inflammatory, and antiangiogenic effects.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e403225"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats. 冰片对缺氧/再氧合H9c2细胞凋亡及心肌缺血再灌注损伤的影响。

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI: 10.1590/acb402225

Hui Zhang, Junfang Dong, Jianwu Zhang, Hongxue Chen, Ting Liu, Ruixue Gan, Jing Wen, Yangyou Li

{"title":"Effects of borneol on apoptosis of hypoxia/reoxygenation H9c2 cells and myocardial ischemia-reperfusion injury rats.","authors":"Hui Zhang, Junfang Dong, Jianwu Zhang, Hongxue Chen, Ting Liu, Ruixue Gan, Jing Wen, Yangyou Li","doi":"10.1590/acb402225","DOIUrl":"10.1590/acb402225","url":null,"abstract":"Purpose: To explore the protective effects of borneol in myocardial ischemia-reperfusion injury (MIRI) and the mechanism of apoptosis.Methods: Cell viability was detected by CCK-8. The total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) leakage of cells were tested by biochemical assay kit. Detection of apoptosis was by flow cytometry. Serum levels of creatine kinase isoenzyme MB (CK-MB), LDH, and cardiac troponin I (cTnI) were detected by enzyme-linked immunosorbent assay. Myocardial infarction area and pathological changes were observed via 2,3,5-triphenyltetrazolium chloride (TTC) staining and hematoxylin and eosin staining. The expressions of apoptosis-related proteins in cells and myocardial tissues were detected by Western blot.Results: H9c2 cell viability was significantly increased by pretreatment with 16 and 32 μg/mL of borneol. Borneol pretreatment significantly increased the T-SOD levels and reduced LDH leakage and apoptosis. In MIRI rats, borneol pretreatment significantly reduced serum levels of CK-MB, LDH and cTnI, decreased myocardial infarction area, and improved myocardial injury in different degree. Western blot results showed that borneol pretreatment significantly reduced the expression of Bcl-2-associated X protein (Bax) and Cysteine-aspartate protease-3 (Caspase-3) in cells and myocardial tissues of rats.Conclusion: Borneol can protect myocardial injury cells and mitigate MIRI by inhibiting cardiomyocyte apoptosis.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402225"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia. 血脂异常的肥胖Zucker大鼠肝脏体视学分析。

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI: 10.1590/acb402325

Silvio Pires Gomes, Gabriela Salim de Castro, Vinicius Pedro Silva de Oliveira, Bruno Cogliati, Andressa Galvão da Silva Iacopino, Ivanir Santana de Oliveira Pires, Bruno Cesar Schimming, Fernanda Gosuen Gonçalves Dias, José Roberto Kfoury Junior, Tais Harumi de Castro Sasahara

{"title":"Hepatic stereological analysis in obese Zucker rats (Leprfa) with dyslipidemia.","authors":"Silvio Pires Gomes, Gabriela Salim de Castro, Vinicius Pedro Silva de Oliveira, Bruno Cogliati, Andressa Galvão da Silva Iacopino, Ivanir Santana de Oliveira Pires, Bruno Cesar Schimming, Fernanda Gosuen Gonçalves Dias, José Roberto Kfoury Junior, Tais Harumi de Castro Sasahara","doi":"10.1590/acb402325","DOIUrl":"10.1590/acb402325","url":null,"abstract":"Purpose: To characterize histologically and stereologically the hepatic steatosis in obese Zucker (fat, n = 6, with a mutation in the leptin receptor - Leprfa) and control Zucker (lean, n = 6) rats, analyzing macroscopic and microscopic differences to understand the influence of obesity on hepatic pathology.Methods: Zucker rats were fed standard chow for 90 days. Macroscopic, qualitative, and histoquantitative (stereological) approaches were used, involving body and liver weight measurement, morphological analysis, and histopathological classification of metabolic dysfunction-associated steatotic liver disease.Results: Zucker fat rats had higher body weight (p = 0.0022), liver weight (p = 0.0022), serum total cholesterol (p = 0.0022), and triacylglycerol (p = 0.0022) compared to Zucker lean rats. Stereological analysis showed that hepatocyte volume density (p = 0.0022) and total hepatocyte volume (p = 0.0001) were lower, and the volume density (p = 0.002) and total volume of steatosis (p = 0.002) were higher in Zucker fat rats compared to lean rats.Conclusion: The findings indicated that obesity induces significant alterations in the hepatic morphology of Zucker rats, showing that hepatocyte volume is lower in obese animals. This study reinforces the utility of the obese Zucker rat model to investigate the effects of obesity on liver health and suggests hepatic steatosis requires therapeutic strategies focused on modulating these parameters.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402325"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Public health system expenditure on motor vehicle collisions in Brazil: an ecological study. 巴西公共卫生系统在机动车碰撞上的支出：一项生态学研究。

Acta cirurgica brasileira Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI: 10.1590/acb402525

Sofia Wagemaker Viana, Ayla Gerk, Sofia Schmitt Schlindwein, Enzzo Marrazzo, Brenda Feres, Lívia Ribeiro, Madeleine Carroll, David Patrick Mooney, Gabriel Schnitman, Cristina Pires Camargo

{"title":"Public health system expenditure on motor vehicle collisions in Brazil: an ecological study.","authors":"Sofia Wagemaker Viana, Ayla Gerk, Sofia Schmitt Schlindwein, Enzzo Marrazzo, Brenda Feres, Lívia Ribeiro, Madeleine Carroll, David Patrick Mooney, Gabriel Schnitman, Cristina Pires Camargo","doi":"10.1590/acb402525","DOIUrl":"10.1590/acb402525","url":null,"abstract":"Purpose: To assess the cost of traffic accidents in Brazil and the impact of age/location.Methods: All patients admitted to a Brazilian hospital due to traffic accidents from January 2012 to December 2022 and cost of hospital services were obtained from the Department of Information Technology of the Unified Health System. Demographic data were collected in the Brazilian Institute of Geography and Statistics database. Parametric and nonparametric data were analyzed. The Kruskal-Wallis' test and a post-hoc test were used for data comparison. The ARIMA linear regression method for trend estimation.Results: In Brazil, 1.6 million individuals were involved in traffic accidents between 2012-2022, resulting in a cumulative hospital expenditure of US$ 38 million. The average hospital admission cost during this time was US$ 239.66, but no correlation was found between the cost per capita and driver population density increase. Hospitalization in the Midwest/South was higher.Conclusion: The economic impact of traffic accidents on the Brazilian public health system is significant. With a high number of victims admitted annually and evident regional and age-related disparities, there is a clear need for comprehensive and cost-effective healthcare strategies.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"40 ","pages":"e402525"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0