Acta cirurgica brasileira最新文献_第10页

Public and private fundraising as a tool for professional development: What is fundraising? 公共和私人筹款作为专业发展的工具:什么是筹款?

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb388123

Everson Luiz de Almeida Artifon, Mariana Gonçalves Magon, Marcio Roberto Facanali Junior, Edna Frasson de Souza Montero

引用次数: 0

WD repeat domain 5 promotes the development of late-onset preeclampsia by activating nuclear factor kappa B. WD重复结构域5通过激活核因子κ B促进迟发性子痫前期的发展。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb386223

Xudong Zhao, Fengyun Su, Fanhua Kong, Juan Su, Xiaojing Yang, Lei Li, Aihua Li, Qinwen Li

{"title":"WD repeat domain 5 promotes the development of late-onset preeclampsia by activating nuclear factor kappa B.","authors":"Xudong Zhao, Fengyun Su, Fanhua Kong, Juan Su, Xiaojing Yang, Lei Li, Aihua Li, Qinwen Li","doi":"10.1590/acb386223","DOIUrl":"10.1590/acb386223","url":null,"abstract":"Purpose: Over-activation of nuclear factor kappa B (NF-κB) was proven to be involved in the pathogenesis of preeclampsia. However, its regulation mechanism is not clear yet. This paper explored the role of WD repeat domain 5 (WDR5) in the development of late-onset preeclampsia and its relationship with NF-κB.Methods: WDR5 expression was detected in normal placentas and placentas from late-onset preeclampsia patients. CCK-8 and colony formation assays were conducted to appraise the proliferative ability of trophoblast. Migration and invasion were observed by wound healing and transwell assays. The interaction between WDR5 and NF-κB inhibitor I-kappa-B-alpha (IkBa) was verified by Co-immunoprecipitation analysis. Immunofluorescence was used to analyze the activation of NF-κB. Finally, we tested the role of WDR5 using the mice late-onset preeclampsia model.Results: WDR5 was highly expressed in the placentas of late-onset preeclampsia patients. WDR5 overexpression suppressed cell proliferation, migration, and invasion in trophoblast. WDR5 could interact with IkBa to activate NF-κB. Knockdown of NF-κB counteracted the anti-proliferative and anti-metastatic effects of WDR5 overexpression in trophoblast. In-vivo studies suggested that targeting WDR5 combated late-onset preeclampsia development.Conclusions: Our finding provides new insights into the role of WDR5 in late-onset preeclampsia development.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e386223"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fasudil mediates neuroprotection in ischemia/reperfusion by modulating the ROCK-PPARα-NOX axis. 法舒地尔通过调节ROCK-PPARα-NOX轴介导缺血/再灌注神经保护。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb387023

Xitong Yang, Guangming Wang

{"title":"Fasudil mediates neuroprotection in ischemia/reperfusion by modulating the ROCK-PPARα-NOX axis.","authors":"Xitong Yang, Guangming Wang","doi":"10.1590/acb387023","DOIUrl":"10.1590/acb387023","url":null,"abstract":"Purpose: Cerebral ischemia-reperfusion (I/R) is a neurovascular disorder that leads to brain injury. In mice, Fasudil improves nerve injury induced by I/R. However, it is unclear if this is mediated by increased peroxisome proliferator-activated receptor-α (PPARα) expression and reduced oxidative damage. This study aimed to investigate the neuroprotective mechanism of action of Fasudil.Methods: MCAO (Middle cerebral artery occlusion) was performed in male C57BL/6J wild-type and PPARα KO mice between September 2021 to April 2023. Mice were treated with Fasudil and saline; 2,3,5-Triphenyltetrazolium chloride (TTC) staining was performed to analyze cerebral infarction. PPARα and Rho-associated protein kinase (ROCK) expression were detected using Western blot, and the expression of NADPH subunit Nox2 mRNA was detected using real-time polymerase chain reaction. The NADPH oxidase activity level and reactive oxygen species (ROS) content were also investigated.Results: After cerebral ischemia, the volume of cerebral necrosis was reduced in wild-type mice treated with Fasudil. The expression of PPARα was increased, while ROCK was decreased. Nox2 mRNA expression, NADPH oxidase activity, and ROS content decreased. There were no significant changes in cerebral necrosis volumes, NADPH oxidase activity, and ROS content in the PPARα KO mice treated with Fasudil.Conclusions: In mice, the neuroprotective effect of Fasudil depends on the expression of PPARα induced by ROCK-PPARα-NOX axis-mediated reduction in ROS and associated oxidative damage.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e387023"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saffron reduces the liver fibrosis in mice by inhibiting the JAK/STAT3 pathway. 藏红花通过抑制JAK/STAT3通路减少小鼠肝纤维化。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb385823

Lijuan Huang, Yan Han, Zhi Wang, Qiao Qiu, Sichen Yue, Qingmin Zhou, Wei Su, Jianhui Yan

{"title":"Saffron reduces the liver fibrosis in mice by inhibiting the JAK/STAT3 pathway.","authors":"Lijuan Huang, Yan Han, Zhi Wang, Qiao Qiu, Sichen Yue, Qingmin Zhou, Wei Su, Jianhui Yan","doi":"10.1590/acb385823","DOIUrl":"10.1590/acb385823","url":null,"abstract":"Purpose: Chronic inflammation in the liver is a key trigger for liver injury and fibrosis in various liver diseases. Given the anti-inflammatory and antioxidant effects of Saffron, this study aimed to investigate the pharmacological effects of Saffron on hepatic inflammation and fibrosis.Methods: The mice model of hepatic fibrosis was constructed using CCl4, and Saffron was administered at low (10 mg/kg) and high (20 mg/kg) doses by gavage. Then, the changes in liver function, liver inflammation and fibrosis markers were evaluated. The effects and mechanisms of Saffron on hepatic stellate cells were further investigated in in-vitro experiments.Results: Saffron improved liver function, reduced liver inflammation and attenuated liver fibrosis in a dose-dependent manner in hepatic fibrosis mice. Furthermore, Western blotting showed that Saffron significantly inhibited JAK/STAT3 phosphorylation in fibrotic livers.Conclusions: Saffron can attenuate liver fibrosis by inhibiting the JAK/STAT3 pathway and the activation of hepatic stellate cell, providing a theoretical basis for the development of new anti-fibrotic drugs.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e385823"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vasopressin in vasoplegic shock in surgical patients: systematic review and meta-analysis. 血管麻痹性休克手术患者的血管加压素:系统回顾和荟萃分析。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb387523

Taís Felix Szeles, Juliano Pinheiro de Almeida, José Arnaldo Shiomi da Cruz, Everson Luiz Almeida Artifon

{"title":"Vasopressin in vasoplegic shock in surgical patients: systematic review and meta-analysis.","authors":"Taís Felix Szeles, Juliano Pinheiro de Almeida, José Arnaldo Shiomi da Cruz, Everson Luiz Almeida Artifon","doi":"10.1590/acb387523","DOIUrl":"10.1590/acb387523","url":null,"abstract":"Purpose: Vasoplegia, or vasoplegic shock, is a syndrome whose main characteristic is reducing blood pressure in the presence of a standard or high cardiac output. For the treatment, vasopressors are recommended, and the most used is norepinephrine. However, new drugs have been evaluated, and conflicting results exist in the literature.Methods: This is a systematic review of the literature with meta-analysis, written according to the recommendations of the PRISMA report. The SCOPUS, PubMed, and ScienceDirect databases were used to select the scientific articles included in the study. Searches were conducted in December 2022 using the terms \"vasopressin,\" \"norepinephrine,\" \"vasoplegic shock,\" \"postoperative,\" and \"surgery.\" Meta-analysis was performed using Review Manager (RevMan) 5.4. The endpoint associated with the study was efficiency in treating vasoplegic shock and reduced risk of death.Results: In total, 2,090 articles were retrieved; after applying the inclusion and exclusion criteria, ten studies were selected to compose the present review. We found no significant difference when assessing the outcome mortality comparing vasopressin versus norepinephrine (odds ratio = 1.60; confidence interval 0.47-5.50), nor when comparing studies on vasopressin versus placebo. When we analyzed the length of hospital stay compared to the use of vasopressin and norepinephrine, we identified a shorter length of hospital stay in cases that used vasopressin; however, the meta-analysis did not demonstrate statistical significance.Conclusions: Considering the outcomes included in our study, it is worth noting that most studies showed that using vasopressin was safe and can be considered in managing postoperative vasoplegic shock.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e387523"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of laser fluorescence spectroscopy in livers of rats with hypothermic ischemia. 低温缺血大鼠肝脏激光荧光光谱研究。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb386023

Allison Takeo Tsuge, Jaqueline de Jesus Pereira, José Dirceu Vollet-Filho, Márcia Saldanha Kubrusly, Flávio Henrique Ferreira Galvão, Orlando Nascimento Ribeiro, Camila Rodrigues Moreno, Renata Nishiyama Ikegami, Eleazar Chaib, Orlando de Castro E Silva

{"title":"Study of laser fluorescence spectroscopy in livers of rats with hypothermic ischemia.","authors":"Allison Takeo Tsuge, Jaqueline de Jesus Pereira, José Dirceu Vollet-Filho, Márcia Saldanha Kubrusly, Flávio Henrique Ferreira Galvão, Orlando Nascimento Ribeiro, Camila Rodrigues Moreno, Renata Nishiyama Ikegami, Eleazar Chaib, Orlando de Castro E Silva","doi":"10.1590/acb386023","DOIUrl":"10.1590/acb386023","url":null,"abstract":"Purpose: After partial hepatectomy (PH), the remaining liver (RL) undergoes regenerative response proportional to the host. Limited literature exists on hepatic viability after tissue injury during hypothermic preservation. Spectroscopy measures cellular fluorescence and is explored for tissue characterization and parameter investigation. This study aimed to assess fluorescence analysis (spectroscopy) in evaluating liver viability and its relationship with hepatic tissue regeneration 24 hours after PH. Additionally, we analyzed liver regeneration in RL after 70% partial hepatectomy under hypothermic conditions with laser irradiation.Methods: Fifty-six Wistar rats were divided into four groups: total non-perfused liver (control), total perfused liver, partial hepatectomy \"in situ\", and partial hepatectomy \"ex situ\". Tissue analysis was performed at 0 and 24 hours using spectroscopy with laser devices emitting at 532 (green) and 405 nm (violet).Results: Spectroscopy identified tissue viability based on consistent results with Ki67 staining. The fluorescence spectra and Ki67 analysis displayed similar patterns, linking proliferative activity and absorption intensity.Conclusions: Fluorescence spectroscopy proves to be promising for real-time analysis of cellular activity and viability. Metabolic activity was observed in groups of live animals and hypothermically preserved samples, indicating cellular function even under blood deprivation and hypothermic conditions.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e386023"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the need for routine intraoperative cell salvage in liver transplantation. 肝移植术中常规细胞抢救的必要性评估。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb386423

Claudianne Maia de Farias Lima, Tatyane Oliveira Rebouças, Luciana Maria de Barros Carlos, Juliana Bezerra Frota Oliveira, Eulene Lima da Silva, Janaína Soares Alves, Clébia Azevedo de Lima, Felipe Pantoja Mesquita, Jannison Karly Cavalcante Ribeiro, Pedro Everson Alexandre de Aquino, Denise Menezes Brunetta, José Huygens Parente Garcia, Antonio Brazil Viana Júnior

{"title":"Assessment of the need for routine intraoperative cell salvage in liver transplantation.","authors":"Claudianne Maia de Farias Lima, Tatyane Oliveira Rebouças, Luciana Maria de Barros Carlos, Juliana Bezerra Frota Oliveira, Eulene Lima da Silva, Janaína Soares Alves, Clébia Azevedo de Lima, Felipe Pantoja Mesquita, Jannison Karly Cavalcante Ribeiro, Pedro Everson Alexandre de Aquino, Denise Menezes Brunetta, José Huygens Parente Garcia, Antonio Brazil Viana Júnior","doi":"10.1590/acb386423","DOIUrl":"10.1590/acb386423","url":null,"abstract":"Purpose: This study aimed to assess the necessity of routine intraoperative cell salvage in liver transplantations.Methods: A total of 327 liver transplants performed between 2014 and 2016 was included in the analysis. Patient data, including pre-transplant examinations, intraoperative red blood cell transfusions, and procedural information, were collected.Results: The median age of the patients was 54 years old, with 67% (219) being male. The most prevalent ABO blood type was O, accounting for 48% (155) of cases. The leading causes of liver disease were hepatitis C (113 cases, 34.6%) and alcohol-related liver disease (97 cases, 29.7%). Out of the 327 liver transplants, allogeneic red blood cell transfusions were administered in 110 cases (34%) with a median of two units of red blood cells per case. Cell salvage was employed in 237 transplants (73%), and successful blood recovery was achieved in 221 cases (93%). Among the group that recovered more than 200 mL of blood, the median volume of recovered blood was 417 mL, with no transfusion of allogeneic blood required. A total of 90 transplants was performed without utilizing cell salvage, and, among these cases, 19 required blood transfusions, with a median of zero units transfused.Conclusions: This study suggests that routine cell salvage is unnecessary for all liver transplantations. The most suitable indication for its use is in patients presenting with portal vein thrombosis and abnormal creatinine levels.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e386423"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of three laparoscopic cholecystectomy techniques in rabbit's model. 三种腹腔镜胆囊切除术方法在兔模型中的比较评价。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb383523

Monica Carolina Nery Wittmaack, Maria Eduarda Bastos Andrade Moutinho Conceição, María Camila Maldonado Vera, Rachel Inamassu Faccini, Guilherme Sembenelli, Gabriel Luiz Montanhim, Mareliza Possa de Menezes, Fabiana Del Lama Rocha, Luiz Paulo Nogueira Aires, Paola Castro Moraes

{"title":"Comparative evaluation of three laparoscopic cholecystectomy techniques in rabbit's model.","authors":"Monica Carolina Nery Wittmaack, Maria Eduarda Bastos Andrade Moutinho Conceição, María Camila Maldonado Vera, Rachel Inamassu Faccini, Guilherme Sembenelli, Gabriel Luiz Montanhim, Mareliza Possa de Menezes, Fabiana Del Lama Rocha, Luiz Paulo Nogueira Aires, Paola Castro Moraes","doi":"10.1590/acb383523","DOIUrl":"10.1590/acb383523","url":null,"abstract":"Purpose: The aim of this randomized study was to compare the complications and perioperative outcome of three different techniques of laparoscopic cholecystectomy (LC). Changes in the liver function test after LC techniques were investigated. Also, we compared the degree of postoperative adhesions and histopathological changes of the liver bed.Methods: Thirty rabbits were divided into three groups: group A) Fundus-first technique by Hook dissecting instrument and Roeder Slipknot applied for cystic duct (CD) ligation; group B) conventional technique by Maryland dissecting forceps and electrothermal bipolar vessel sealing (EBVS) for CD seal; group C) conventional technique by EBVS for gallbladder (GB) dissection and CD seal.Results: Group A presented a longer GB dissection time than groups B and C. GB perforation and bleeding from tissues adjacent to GB were similar among tested groups. Gamma-glutamyl transferase and alkaline phosphatase levels increased (p ≤ 0.05) on day 3 postoperatively in group A. By the 15th postoperative day, the enzymes returned to the preoperative values. Transient elevation of hepatic transaminases occurred after LC in all groups. Group A had a higher adherence score than groups B and C and was associated with the least predictable technique.Conclusions: LC can be performed using different techniques, although the use of EBVS is highly recommended.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e383523"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of deep resection of endometriosis in the pelvis on urodynamic parameters. 骨盆子宫内膜异位症深度切除对尿动力学参数的影响。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb386323

Jardel Cavalcante de Farias, Maria do Desterro Soares Brandão Nascimento, Plínio da Cunha Leal, Caio Márcio Barros de Oliveira, Ed Carlos Rey Moura

{"title":"Impact of deep resection of endometriosis in the pelvis on urodynamic parameters.","authors":"Jardel Cavalcante de Farias, Maria do Desterro Soares Brandão Nascimento, Plínio da Cunha Leal, Caio Márcio Barros de Oliveira, Ed Carlos Rey Moura","doi":"10.1590/acb386323","DOIUrl":"10.1590/acb386323","url":null,"abstract":"Purpose: To evaluate the effects of deep resection of endometriosis in the posterior pelvic region on urodynamic parameters.Methods: A prospective observational study conducted with female patients diagnosed with deep pelvic endometriosis before and after endometriosis resection surgery. Clinical history, image exams, the Female Lower Urinary Tract Symptoms questionnaire, urodynamic examination, cystometry, and voiding study were evaluated.Results: Patients aged 30-39 years old, operative duration of 132.5 minutes, and 2.7 days of hospital stay. Uroflowmetry and cystometry showed tendency for an increase after the surgery in the flow duration, time to maximum flow, and first voiding desire and decreased residual volume and maximum cystometric capacity. Opening, maximum urinary flow, and maximum flow pressure decreased at T1, and the closing parameters increased, although statistically non significant. The variables decreased at T1 in the urodynamic, except for detrusor overactivity. Although we observed a reasonable number of low bladder compliance and abnormal bladder sensation, the results were maintained at T1. General scores for filling and incontinence showed a significant decrease after surgery.Conclusions: A significant response in the patient's perception of urinary function was demonstrated after surgery. It is observed that the surgical procedure did not affect the uroflowmetric and cystometric characteristics of the evaluated patients.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e386323"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagy and anti-inflammation ameliorate diabetic neuropathy with Rilmenidine. 利美尼定可改善糖尿病神经病变的自噬和抗炎作用。

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI: 10.1590/acb387823

Mehmet Burak Yalçın, Ejder Saylav Bora, Adem Çakır, Sabiye Akbulut, Oytun Erbaş

{"title":"Autophagy and anti-inflammation ameliorate diabetic neuropathy with Rilmenidine.","authors":"Mehmet Burak Yalçın, Ejder Saylav Bora, Adem Çakır, Sabiye Akbulut, Oytun Erbaş","doi":"10.1590/acb387823","DOIUrl":"10.1590/acb387823","url":null,"abstract":"Purpose: To evaluate the neuroprotective effects of Rilmenidine on diabetic peripheral neuropathy (DPN) in a rat model of diabetes induced by streptozotocin (STZ).Methods: STZ (60 mg/kg) was administered to adult Sprague-Dawley rats to induce diabetes. On the 30th day after STZ administration, electromyography (EMG) and motor function tests confirmed the presence of DPN. Group 1: Control (n = 10), Group 2: DM + 0.1 mg/kg Rilmenidine (n = 10), and Group 3: DM + 0.2 mg/kg Rilmenidine (n = 10) were administered via oral lavage for four weeks. EMG, motor function test, biochemical analysis, and histological and immunohistochemical analysis of sciatic nerves were then performed.Results: The administration of Rilmenidine to diabetic rats substantially reduced sciatic nerve inflammation and fibrosis and prevented electrophysiological alterations. Immunohistochemistry of sciatic nerves from saline-treated rats revealed increased perineural thickness, HMGB-1, tumor necrosis factor-α, and a decrease in nerve growth factor (NGF), LC-3. In contrast, Rilmendine significantly inhibited inflammation markers and prevented the reduction in NGF expression. In addition, Rilmenidine significantly decreased malondialdehyde and increased diabetic rats' total antioxidative capacity.Conclusions: The findings of this study suggest that Rilmenidine may have therapeutic effects on DNP by modulating antioxidant and autophagic pathways.","PeriodicalId":93850,"journal":{"name":"Acta cirurgica brasileira","volume":"38 ","pages":"e387823"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0