Protective effect of proteins extracted from Plumeria pudica latex on ethanol-induced gastric injury in mice.

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Acta cirurgica brasileira Pub Date : 2025-06-06 eCollection Date: 2025-01-01 DOI:10.1590/acb404025

Lucas Arruda Moita, Bruna da Silva Souza, Naylla Veras de Moraes Oliveira, Ana Clara Silva Sales, Lucas Eduardo Silva Oliveira, Ana Patrícia de Oliveira, Francisca Beatriz Melo Sousa, Jand-Venes Rolim Medeiros, Jefferson Soares de Oliveira

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Abstract

Purpose: To evaluate proteins from Plumeria pudica latex (LPPp) for their protective effect against ethanol-induced gastric injury in mice.

Methods: The LPPp fraction was obtained by collecting P. pudica latex in tubes containing distilled water, followed by centrifugation and dialysis. The female Swiss mice (Mus musculus) received saline or LPPp (40 mg/kg) intraperitoneally 1 hour before oral administration of 500 μL of 50% ethanol. One hour later, the animals were euthanized, and their stomachs were removed for evaluation of tissue lesion area, histopathological analysis, and measurements of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and nitrate/nitrite (NO3/NO2). An independent experiment assessed the effect of LPPp on gastric mucus production.

Results: The LPPp-treated animals showed a significant reduction in the mean injured areas of gastric tissue (0.73 ± 1.01 mm2) compared to the ethanol group (37.99 ± 3.11 mm2). Histopathological analysis revealed significant preservation of tissue architecture in the LPPp group compared to ethanol group. Additionally, LPPp maintained tissue levels of MDA, GSH, SOD, and NO3/NO2 comparable to the saline group and influenced gastric mucus production favorably (p < 0.05).

Conclusion: These results suggested that LPPp has a protective effect against ethanol-induced gastric lesions, likely involving antioxidant activity and increased gastric mucus production.

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鸡蛋花乳胶提取蛋白对乙醇致小鼠胃损伤的保护作用。

目的：探讨鸡蛋鸡乳蛋白对乙醇致小鼠胃损伤的保护作用。方法：用蒸馏水管收集耻骨假体乳胶，离心、透析得到LPPp部分。雌性瑞士小鼠（小家鼠）在口服50%乙醇500 μL前1小时腹腔注射生理盐水或LPPp （40 mg/kg）。1小时后，对大鼠实施安乐死，取胃进行组织病变面积评估、组织病理学分析、丙二醛（MDA）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、硝酸盐/亚硝酸盐（NO3/NO2）测定。一项独立实验评估了LPPp对胃粘液产生的影响。结果：与乙醇组（37.99±3.11 mm2）相比，lpp组胃组织平均损伤面积（0.73±1.01 mm2）明显减少。组织病理学分析显示，与乙醇组相比，LPPp组明显保留了组织结构。此外，与生理盐水组相比，LPPp维持了组织中MDA、GSH、SOD和NO3/NO2的水平，并有利地影响了胃粘液的产生（p < 0.05）。结论：这些结果表明，LPPp对乙醇诱导的胃病变具有保护作用，可能涉及抗氧化活性和增加胃粘液生成。

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Acta cirurgica brasileira

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