BMC Microbiology最新文献_第3页

Sustainable waste-based fermentation for enhanced β-carotene biosynthesis by Blakeslea trispora and functional evaluation of the extracted pigment. 三孢黑霉可持续废物发酵促进β-胡萝卜素生物合成及提取色素的功能评价。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-30 DOI: 10.1186/s12866-026-04971-3

Negin Ghazanfari, Ali Motlagh Hosseini, Fereshteh Falah, Motahareh Nasiri, Farideh Tabatabaei Yazdi, Mohamad Reza Razavi, Alireza Vasiee

{"title":"Sustainable waste-based fermentation for enhanced β-carotene biosynthesis by Blakeslea trispora and functional evaluation of the extracted pigment.","authors":"Negin Ghazanfari, Ali Motlagh Hosseini, Fereshteh Falah, Motahareh Nasiri, Farideh Tabatabaei Yazdi, Mohamad Reza Razavi, Alireza Vasiee","doi":"10.1186/s12866-026-04971-3","DOIUrl":"https://doi.org/10.1186/s12866-026-04971-3","url":null,"abstract":"Background: Valorization of agri-food waste as nutrient-rich substrates provides a sustainable strategy for microbial bioprocessing. Blakeslea trispora is a well-known fungal producer of natural β-carotene; however, its performance on low-cost industrial waste streams remains underexplored.Results: In this study, dairy sludge and soybean meal were evaluated as alternative carbon and nitrogen sources for β-carotene biosynthesis by B. trispora. Response Surface Methodology (RSM) identified an optimized medium containing 12% dairy sludge, 2% soybean meal, and 0.4% yeast extract, yielding 65 g/L biomass and 352 μg/mL β-carotene in laboratory-scale fermentation. The pigment extracted from optimized cultures was confirmed as β-carotene through HPLC, FTIR, and 13C-NMR analyses. Biofunctional assays revealed antimicrobial activity (MIC: 250-500 mg/mL), with greater susceptibility in Gram-positive strains. Moderate antioxidant activity was observed in the DPPH assay (38% inhibition), and cytotoxic evaluation against Caco-2 cells resulted in an IC₅₀ of 400 mg/mL, indicating potential anticancer effects.Conclusion: These findings demonstrate that agri-food wastes can serve as efficient substrates for fungal β-carotene production, supporting cost-effective and sustainable microbial bioprocessing. The functional properties of the extracted β-carotene further highlight its applicability as a bioactive microbial metabolite with antimicrobial, antioxidant, and anticancer potential.","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of AI-enhanced FTIR spectroscopy for species and biovar typing of intracellular pathogenic Brucella spp. 人工智能增强FTIR光谱对细胞内致病性布鲁氏菌种类和生物分型的评价。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-30 DOI: 10.1186/s12866-026-05057-w

Sanja Duvnjak, Maja Dopuđ, Sandra Šuto, Irena Reil, Maja Zdelar-Tuk, Andrzej Mikolajczak, Silvio Špičić

{"title":"Evaluation of AI-enhanced FTIR spectroscopy for species and biovar typing of intracellular pathogenic Brucella spp.","authors":"Sanja Duvnjak, Maja Dopuđ, Sandra Šuto, Irena Reil, Maja Zdelar-Tuk, Andrzej Mikolajczak, Silvio Špičić","doi":"10.1186/s12866-026-05057-w","DOIUrl":"https://doi.org/10.1186/s12866-026-05057-w","url":null,"abstract":"Background: Rapid and accurate identification of intracellular pathogenic Brucella species and biovars is essential for effective public health surveillance, outbreak control, and preservation of animal and human health. While traditional biotyping remains the gold standard for biovar classification, it is time-consuming, technically demanding, dangerous, and costly. We validated artificial intelligence (AI)-coupled Fourier-transform infrared (FTIR) spectroscopy for Brucella biotyping as a faster, automated, and cost-effective alternative.Results: One hundred and sixty-three strains of different species and biovars, originating from humans and animals, were assessed. The FTIR achieved high accuracy across all assessed species, achieving 95-100% sensitivity and specificity. On the biovar level, differentiating also vaccine from wild type strains, it achieved 80-100% sensitivity and specificity, depending on the species and the number of available strains for different biovars.Conclusions: Our research shows the identification of the FTIR as a highly robust and automated high-throughput method. This approach generates real-time data that shows potential for real-time epidemiological surveillance and allows early detection of outbreaks caused by zoonotic pathogens. By integrating classical microbiological methods with AI, the method shows great promise for its applicability in routine laboratory work and a high-throughput, fast, simple, safe, and cost-effective strategy for epidemiological surveillance of intracellular pathogenic Brucella bacteria on a global scale.","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug repurposing: antimicrobial and antibiofilm effects of MLS0315771 against Gram-positive bacteria. 药物再利用：MLS0315771对革兰氏阳性菌的抗菌和抗菌膜作用。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-29 DOI: 10.1186/s12866-026-05074-9

Zhichao Xu, Yuanyuan Tang, Junhua Ma, Peiyu Li, Zewen Wen, Zhijian Yu, Bing Bai, Zhong Chen, Shiqing Han, Tieying Hou

引用次数: 0

Response of rhizosphere bacterial and fungal communities to reduced light intensity in tomato plant. 番茄根际细菌和真菌群落对弱光的响应

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-29 DOI: 10.1186/s12866-026-05038-z

Fei Sun, Xinjie Pan, Mingming Chen, Rahmatullah Khan, Hongyu Yang, Shouwei Liu, Jingyu Zhang, Xingang Zhou, Fengzhi Wu, Danmei Gao

{"title":"Response of rhizosphere bacterial and fungal communities to reduced light intensity in tomato plant.","authors":"Fei Sun, Xinjie Pan, Mingming Chen, Rahmatullah Khan, Hongyu Yang, Shouwei Liu, Jingyu Zhang, Xingang Zhou, Fengzhi Wu, Danmei Gao","doi":"10.1186/s12866-026-05038-z","DOIUrl":"https://doi.org/10.1186/s12866-026-05038-z","url":null,"abstract":"Background: In protected vegetable cultivation, shading is commonly used to alleviate high light stress by reducing light intensity. However, the effects of shading on the structure and composition of soil microbial communities remain unclear.Methods: To compare the effects of no-shading and shading conditions on bacterial and fungal communities in the tomato rhizosphere, high-throughput sequencing was performed on the 16S rRNA gene and the internal transcribed spacer (ITS) amplicons.Results: The results showed that the alpha diversity of the fungal community and the Shannon index of bacteria were reduced by shading. The relative abundances of photoautotrophic microorganisms (such as the phylum Cyanobacteria and its class Cyanophyceae) was significantly reduced, while the relative abundance of the class Agaricomycetes was significantly increased. Notably, shading increased the relative abundances of microbial groups known to be associated with plant performance and soil nutrient transformation, such as Sphingomonas, Pseudomonas, and Mortierella. In contrast, the relative abundance of the potential plant pathogenic fungus Gibberella decreased.Conclusion: Overall, shading altered the composition of the tomato rhizosphere microbial community by enriching specific groups while inhibiting potential pathogenic and photoautotrophic microorganisms. This study investigated the response of rhizosphere microorganisms to shading from a microbiological perspective, providing a scientific basis for optimizing light environment management in facility cultivation.","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal activity of curcumin nanoparticles and Saccharomyces cerevisiae against aflatoxigenic Aspergillus flavus isolated from broiler chickens rations in Egypt. 姜黄素纳米颗粒和酿酒酵母对埃及肉鸡口粮中产黄曲霉的抗真菌活性研究

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-29 DOI: 10.1186/s12866-026-04963-3

Usama Hassan Abo-Shama, Eman Mahmoud El-Diasty, Doaa Ebrahim Mohamed, Reem Mohamed Alsaadawy, Haitham Helmy Sayed

引用次数: 0

A novel yeast extract from a multi-stress-resistant Saccharomyces cerevisiae confers potent antioxidant and UV-protective activities to skin cells. 一种新的酵母提取物从多重抗应激酿酒酵母赋予有效的抗氧化和紫外线保护活性的皮肤细胞。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-29 DOI: 10.1186/s12866-026-05047-y

Xueyu Yang, Xinyi Zhang, Minjia Yuan, Qi Li, Xinchen Teng

{"title":"A novel yeast extract from a multi-stress-resistant Saccharomyces cerevisiae confers potent antioxidant and UV-protective activities to skin cells.","authors":"Xueyu Yang, Xinyi Zhang, Minjia Yuan, Qi Li, Xinchen Teng","doi":"10.1186/s12866-026-05047-y","DOIUrl":"https://doi.org/10.1186/s12866-026-05047-y","url":null,"abstract":"Yeast extract derived from Saccharomyces cerevisiae is a well-established cosmetic ingredient known for its diverse biological benefits, including UV protection. However, the directed optimization of yeast strains to obtain extracts with enhanced UVA protection for skin cells has not been previously reported. In this study, we developed a novel directed screening strategy to isolate a UVA-tolerant mutant strain, A154, which also exhibits multi-stress tolerance, including resistance to heat, oxidative stress, and UV radiation. Yeast extract from the A154 strain demonstrated significantly stronger UVA-protective and reactive oxygen species (ROS)-scavenging properties in human skin cell lines compared with the parental strain. In the presence of 0.5% A154 yeast extract, cell viability was increased by approximately 1.7-fold (HFF, 120 kJ/m2), 1.4-fold (A431, 120 kJ/m2), and 1.9-fold (HaCaT, 360 kJ/m2) after UVA irradiation. Further analysis of functional components in yeast extracts from the A154 and parental strains revealed an approximately 1.5-fold increase in glutathione and a 1.6-fold increase in polysaccharides. Additionally, whole-genome sequencing coupled with functional validation using knockout strains identified multiple previously uncharacterized genes in the A154 strain that are unrelated to DNA repair and contribute to UVA tolerance. In conclusion, yeast extract derived from A154 exhibits potent UV-protective and antioxidant activities, highlighting its potential applications in mitigating photoaging and UV-induced skin damage.","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-pandemic resurgence and co-detectiondynamics of respiratory pathogens revealed by syndromic multiplex PCR. 综合征多重PCR揭示的大流行后呼吸道病原体的死灰复灭和共同检测动态。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-28 DOI: 10.1186/s12866-026-05081-w

Ismail Selçuk Aygar

引用次数: 0

CgCFEM1 and CgCFEM2 modulate virulence in Colletotrichum gloeosporioides by integrated regulation of TOR and cAMP-PKA signaling pathways. CgCFEM1和CgCFEM2通过综合调控TOR和cAMP-PKA信号通路调控炭疽菌毒力。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-28 DOI: 10.1186/s12866-026-04969-x

Liping Feng, Wenjie Liu, Qiannan Wang, Bang An, Hongli Luo

引用次数: 0

Exploring alterations in the gut resistome in medically treated inflammatory bowel disease patients. 探索经药物治疗的炎症性肠病患者肠道抵抗组的改变。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-28 DOI: 10.1186/s12866-026-05101-9

Jonas Christoffer Lindstrøm, Hilde S Vollan Gjerdrum, Ola B Brynildsrud, Tone Møller Tannæs, Anja Bråthen Kristoffersen, Petr Ricanek, Truls M Leegaard, Jørgen Vildershøj Bjørnholt, Silje Bakken Jørgensen, Hege S Tunsjø, Christine Olbjørn, Trond Espen Detlie, Jørgen Jahnsen, Vendel A Kristensen, Marte Lie Høivik, Johannes R Hov, Aina EFossum Moen

引用次数: 0

Aspergillus flavus glutathione transferase as a potential approach for synthesis of epothilone-glutathione conjugates with enhanced anticancer activity for the drug-resistant cells: characterization and molecular docking analysis. 黄曲霉谷胱甘肽转移酶作为合成具有增强耐药细胞抗癌活性的epothilone-glutathione偶联物的潜在途径：表征和分子对接分析。

IF 4.2 2区生物学

BMC Microbiology Pub Date : 2026-04-28 DOI: 10.1186/s12866-026-05037-0

Ashraf S A El-Sayed, Ahmed A Shindia, Hala Ammar, Eman Fikry, Samar A Khashana, Marwa A Yassin

引用次数: 0