BMC Microbiology最新文献

{"title":"Unraveling the pathogenic potential, virulence traits, and antibiotic resistance genes of multidrug-resistant Streptococcus agalactiae strains retrieved from Nile tilapia.","authors":"Abdelazeem M Algammal, Mahmoud Mabrok, Bian K Almessiry, Banan Atwah, Amenah S Al-Otaibi, Yehia S Mohamed, Sinclair Steele, Mohamed Enany, Geraldine B Dayrit, Fatma M Yousseff, Marwa E Abo Hashem","doi":"10.1186/s12866-025-04405-6","DOIUrl":"10.1186/s12866-025-04405-6","url":null,"abstract":"<p><strong>Background: </strong>Streptococcus agalactiae is implicated in severe infections in humans and causes considerable financial losses in the aquaculture industry, particularly in tilapia farming. Given its dual threat to public health and food security, this study was conducted to comprehensively assess the prevalence, molecular identification, multidrug resistance (MDR) patterns, virulence traits, antimicrobial resistance genes, and pathogenic potential of S. agalactiae strains isolated from Oreochromis niloticus. Notably, this study highlights the detection of critical virulence genes (cfb, hylB, lmb, and cylE), which play essential roles in host invasion and immune evasion. In parallel, the presence of resistance determinants such as pbp1A, aac(6')-aph(2''), tetM or tetK, and ermB or ermA underscores the growing threat of antimicrobial resistance in aquatic environments.</p><p><strong>Methods: </strong>A total of 180 Oreochromis niloticus samples-comprising 90 apparently healthy and 90 moribund fish-were collected from private fish farms in Ismailia, Egypt. Clinical and postmortem (PM) examinations, along with bacteriological analysis, were subsequently performed. The recovered isolates were then subjected to molecular identification via 16 S rRNA gene amplification, antibiotic susceptibility testing via standard protocols, and PCR-based screening for key virulence and antimicrobial resistance genes.</p><p><strong>Results: </strong>All recovered Streptococcus agalactiae isolates were confirmed by the presence of the species-specific 16 S rRNA gene. The overall prevalence of S. agalactiae among the examined O. niloticus was 10% (18/180), with the brain identified as the primary target organ for infection. PCR analysis revealed that the most frequently detected virulence-associated genes among the isolates were cfb (100%), hylB (82.1%), lmb (78.5%), and cylE (57.1%). Additionally, 35.7% of the S. agalactiae isolates exhibited multidrug resistance (MDR) to seven different antimicrobial classes and harbored resistance genes including pbp1A, aac(6')-aph(2''), tetM, and ermB. Another 17.8% of the isolates were MDR to six antimicrobial classes and carried the pbp1A, tetM, and ermA genes. Pathogenicity assays demonstrated a strong correlation between the presence of virulence genes and the observed mortality rates in experimentally infected fish. Notably, mortality sharply increased within the first 7 days post-inoculation, reaching approximately 73.3%, underscoring the high virulence potential of the recovered strains.</p><p><strong>Conclusion: </strong>Briefly, this study highpoints the development of MDR S. agalactiae in O. niloticus, affirming a public health risk. The emerging MDR S. agalactiae strains in O. niloticus frequently harbor the cfb, hylB, lmb, and cylE virulence genes, and the pbp1A, aac(6')aph(2''), tetM or tetK, and ermB or ermA resistance genes.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"629"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of prevalence and phylogenetic classification of Microsporidia MB and insecticide target site insensitivity resistance mutations in Anopheles gambiae s.l. and Anopheles funestus mosquitoes from Busia, Kenya.","authors":"Herzel Tiffany Wandera, Godfrey Nattoh, Daniel Kiboi, Manase Onyango Aloo, Stanley Kitur, Sharon Nyagaka, Malvin Atieno Otieno, Eric Ochomo, Damaris Matoke-Muhia, Jeremy Keith Herren, Luna Kamau","doi":"10.1186/s12866-025-04324-6","DOIUrl":"10.1186/s12866-025-04324-6","url":null,"abstract":"<p><strong>Background: </strong>Microsporidia MB, a Plasmodium-transmission-impairing symbiont in Anopheles arabiensis, has malaria control potential. This study assessed its prevalence and phylogeny in An. gambiae s.l. and An. funestus in Busia, Kenya and investigated the influence of environmental factors on its occurrence. Additionally, the prevalence of key insecticide resistance mutations in these mosquito populations was determined.</p><p><strong>Methods: </strong>Mosquito larvae and adults were collected from three sub-counties in Busia County, Kenya and identified based on morphological characteristics. PCR was used to determine Anopheles species distribution and Microsporidia MB prevalence following DNA extraction from the samples. Insecticide resistance target-site mutations were identified using TaqMan genotyping in a subset of the mosquito samples. Multivariable logistic regression models were used to assess associations of Microsporidia MB infection and ecological factors. Microsporidia MB-positive samples were whole-genome sequenced and phylogenetically analysed.</p><p><strong>Results: </strong>Overall, An. gambiae s.l. (including An. gambiae s.s. and possibly An. coluzzii) comprised 57.3% of samples analysed while An. funestus comprised 25.7% and An. arabiensis 17% and their distribution varied significantly across the three sub-counties (Chi-square, χ² = 577.44, df = 4, p < 0.001). Microsporidia MB prevalence was low to moderate (0 to 6.4%) and highest in An. gambiae s.l. Anopheles gambiae s.l. showed significantly higher odds of infection compared to An. arabiensis (aOR = 5.94, 95% CI: 1.96-26.77, p = 0.006). Larvae reared to adults had significantly lower odds of infection than indoor-collected adults (aOR = 0.48, 95% CI: 0.26-0.86, p = 0.014). Insecticide resistance genotyping revealed high frequencies of kdr-East (94.7%) and kdr-West (60%) mutations in the Anopheles subset analysed, while Ace-1 and GSTe2 mutations were absent. Phylogenetic analysis placed Microsporidia MB isolated from Busia, within Clade IV, closely related to the originally sequenced Ahero reference, but still distinct from other microsporidian clades (I and III).</p><p><strong>Conclusion: </strong>The present study highlights the occurrence of Microsporidia MB in multiple Anopheles vectors associated with malaria suggesting its broader potential as a vector control tool. The high prevalence of kdr mutations indicate a significant challenge to insecticide-based vector control in the region. Further investigation into the phenotypic expression of insecticide resistance in these populations is important. Results of the phylogenetic analysis suggest a common ancestry for Microsporidia MB isolates from Busia with the Ahero reference one, highlighting shared traits with potential for malaria control.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"604"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of a putative novel bacteriocin from Bacillus velezensis G02 and its effects on the intestinal microflora in mice.","authors":"Mengbo Yu, Haotian Ma, Jinju Peng, Yuexia Ding, Qianqian Lin, Fucheng Guo, Yi Ma","doi":"10.1186/s12866-025-04357-x","DOIUrl":"10.1186/s12866-025-04357-x","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"610"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation of the bactericidal and anti-pathogenic efficacy of chemical disinfectants against antibiotic resistant ESKAPE clinical strains.","authors":"Mihaela Magdalena Mitache, Andreea Pîndaru, Ilda Czobor Barbu, Daniela Talapan, Marius Surleac, Ecaterina Sârbu, Luminița Măruțescu, Mariana Carmen Chifiriuc","doi":"10.1186/s12866-025-04273-0","DOIUrl":"10.1186/s12866-025-04273-0","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"592"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving silage characteristics in high-moisture grape residues using cotton stalk as a moisture regulator.","authors":"Ke Xu, Junyu Zhang, Aireti Mirizati, Bate Bayin, Xubiao Han, Lingling Su","doi":"10.1186/s12866-025-04374-w","DOIUrl":"10.1186/s12866-025-04374-w","url":null,"abstract":"<p><p>Grape branches and leaves are promising feedstock for high-quality silage, yet their high moisture content limits effective fermentation. This study aimed to investigate the effect of cotton stalk addition on fermentation quality and microbial characteristics of high-moisture grape branches and leaves silage, providing a basis for the efficient utilization of these resources as animal feed. The experiment included six treatments with different levels of MG (cotton stalk) : 0% (MG0), 10% (MG10), 20% (MG20), 30% (MG30), 40% (MG40), and 50% (MG50). After 60 days of silage fermentation, increasing MG proportion significantly reduced silage moisture content (P < 0.05), while pH and acetic acid (AA) levels significantly increased (P < 0.05). Bacterial community analysis revealed that the dominant genera were mainly Lentilactobacillus, Bacteroides, unclassified_rumen_bacterium, and Romboutsia. Among these, Lentilactobacillus predominated in MG20 group, with a relative abundance of 92.15%. Correlation analysis indicated that Lentilactobacillus was significantly negatively correlated with potentially undesirable bacteria such as Romboutsia and unclassified_Clostridia_UGG_014 (P < 0.01). Network analysis showed that addition of MG altered the bacterial community structure, with MG20 group exhibiting the highest mean degree and graph density, suggesting enhanced microbial interactions. In conclusion, incorporating cotton stalk can significantly improve fermentation quality of high-moisture grape branches and leaves silage while optimizing its microbial community. The best fermentation performance was achieved with 20% cotton stalk addition.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"619"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral and fecal microbiome alterations in pancreatic cancer: insights into potential diagnostic biomarkers.","authors":"Sogol Tavanaeian, Mohammad Mehdi Feizabadi, Sarvenaz Falsafi, Hamid Asadzadeh Aghdaei, Hamidreza Houri","doi":"10.1186/s12866-025-04344-2","DOIUrl":"10.1186/s12866-025-04344-2","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"624"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence factors of Candida spp. isolated from COVID-19 patients: hydrolytic enzyme activity and biofilm formation.","authors":"Ahmad Jabrodini, Somayeh Yazdanpanah, Mina Malekzadeh, Shafigheh Shabanzadeh, Maryam Ahmadyan, Keyvan Pakshir, Kamiar Zomorodian","doi":"10.1186/s12866-025-04351-3","DOIUrl":"10.1186/s12866-025-04351-3","url":null,"abstract":"<p><p>During the coronavirus disease 2019 (COVID-19) pandemic, an increased prevalence of Candida co-infections has been reported. However, data on the virulence factors of Candida spp. isolated from COVID-19 patients remain scarce. This study aimed to assess the virulence factors of Candida spp. isolated from COVID-19 patients and to explore their potential associations with clinical parameters. A total of 71 Candida (C.) strains were analyzed, representing four species: 49 (69.01%) C. albicans strains, 14 (19.71%) C. glabrata strains, 7 (9.85%) C. tropicalis strains, and 1 (1.40%) C. dubliniensis strain. The activities of proteinase, phospholipase, and hemolysin, as well as biofilm-forming capacity, were evaluated. All C. albicans strains and 21 (95.45%) non-albicans Candida (NAC) strains produced proteinase. Phospholipase activity was detected in 46 (93.87%) C. albicans strains and 10 (45.45%) NAC strains. High hemolytic activity was observed in all Candida strains. Biofilm formation was detected in 31 (43.66%) Candida strains, with variable intensities. These findings highlight high levels of hydrolytic enzyme activity among Candida spp. isolated from COVID-19 patients and contribute to the growing understanding of Candida pathogenesis in immunocompromised populations, providing insights for patient management.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"611"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized production and characterization of red dye from Talaromyces purpureogenus PH7 for application as a textile dye.","authors":"Iqbal Hussain, Muhammad Irshad, Anwar Hussain, Muneebur Rahman, Arshad Qasim, Abdulwahed Fahad Alrefaei, Mikhlid H Almutairi, Muhammad Siraj, Sajid Ali, Muhammad Hamayun","doi":"10.1186/s12866-025-04294-9","DOIUrl":"10.1186/s12866-025-04294-9","url":null,"abstract":"<p><p>Switching from use of synthetic dyes in the textile industry to ecofriendly organic dyes will be a good initiative to safeguard the environment. As an attempt to contribute to the cause, this study optimized conditions for the growth of a rhizospheric fungus Talaromyces purpureogenus PH7 (PQ281486) and production of a red dye by it, under lab conditions. Additionally, the dye produced by the strain was applied to cotton and cotton fabrics following the standard staining procedure and the dyed fabrics were subjected to washes with hot and cold water, detergents and continuous sunlight as preliminary tests for its use as a potential dye of the textile industry. Maximum growth of the strain and dye production by it were attained at 28 °C and pH7 using dextrose as source carbon in the Czapek medium. On the other hand, high concentration of sodium chloride significantly reduced growth of the strain as well as production of the red dye by it. During evaluation of color as potential textile dye, it efficiently dyed cotton and cotton fabrics that did not get faded by any of the experimental treatments including exposure to hot/cold water, detergents and sunlight. It's appealing that the effluents generated during culturing of the strain could have an overall beneficial impact on the environment as it produces metabolites with important roles in well-being of plant, like IAA, flavonoids, phenolics, lipids, proteins and sugars, and exhibits strong antioxidant activity. In conclusion, the fungus T. purpureogenus PH7 is a promising candidate for producing a natural, biodegradable yet durable red textile-grade dye in an eco-friendly manner.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"596"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct identification and antimicrobial susceptibility testing of microorganisms from positive blood culture bottles using a membrane filtration method.","authors":"Tsui-Ping Liu, Hui-Fang Wu, Pai-Ling Chang","doi":"10.1186/s12866-025-04337-1","DOIUrl":"10.1186/s12866-025-04337-1","url":null,"abstract":"<p><strong>Background: </strong>Bloodstream infections (BSIs) are a major cause of morbidity and mortality globally. Rapid and accurate pathogen identification and antimicrobial susceptibility testing (AST) are crucial for timely and effective treatment. Conventional blood culture (BC) workflows are time-consuming, typically requiring 18-48 h. Although integrating matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with direct AST protocols is promising, certain limitations remain. Therefore, we aimed to establish a faster and more reliable method for the direct identification and AST of microorganisms from positive BC bottles using a membrane filtration-based protocol.</p><p><strong>Results: </strong>The membrane filtration-based method achieved an overall identification success rate of 76.5%. Rates were highest for Gram-negative bacteria (88.1%), followed by anaerobes (80.0%), Gram-positive cocci (70.2%), and Gram-positive bacilli (43.8%). Yeast identification was unsuccessful. AST results showed strong concordance with those from the conventional method, with essential agreement (EA) exceeding 95% across all groups. For Gram-negative bacteria, EA was 98% and categorical agreement (CA) was 95.4%, with 3.6%, 0.5%, and 0.5% minor, major, and very major errors, respectively. EA and CA were 96.1% and 94.2% for Gram-positive cocci, and 95.5% and 93.4% for Streptococcus spp.</p><p><strong>Conclusions: </strong>The membrane filtration-based method, effective for Gram-negative bacteria, reduced turnaround time by 10-12 h compared to conventional workflows. Further optimization can improve accuracy for Gram-positive bacteria and yeast, offering a promising, streamlined workflow to enhance BSI diagnostics and guide targeted antimicrobial therapy.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"606"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of bacteriophages PAA and PAM and evaluation of their antibiotic synergy against Pseudomonas aeruginosa PAZMYU isolated from urine sample.","authors":"Aneela Nawaz, Nauman Ahmed Khalid, Alina Majid, Muqaddas Shahzadi, Sabeena Zafar, Muhammad Ismail Khan, Nasir Khan, Ali Khalid, Aamer Ali Shah, Malik Badshah, Samiullah Khan","doi":"10.1186/s12866-025-04195-x","DOIUrl":"10.1186/s12866-025-04195-x","url":null,"abstract":"<p><p>Antibiotic resistance has been attributed to both the overuse of current drugs and the lack of availability of newer drugs due to stringent regulatory requirements and reduced commercial incentives. Novel alternative therapies, such as phage treatments, have demonstrated promising outcomes in clinical trials, indicating their potential for treating recalcitrant infections in the future. The objective of this study was to isolate and characterize bacteriophages against a uropathogenic strain of Pseudomonas aeruginosa and evaluate the antimicrobial efficacy of combining sub-inhibitory concentrations of cefepime and meropenem with the isolated bacteriophages. The isolated phages were designated PAA and PAM, both exhibiting icosahedral heads and long non-contractile tails. Both phages maintained stability within Limited pH and temperature ranges. The optimal adsorption times for PAA and PAM were 10 and 20 min, respectively, with PAA demonstrating a short latent time and burst size of 47, while PAM exhibited a burst size of 83. The optimal multiplicity of infection for PAA was 1, and for PAM was 0.1. PAA demonstrated efficacy against 40% of the tested strains of P. aeruginosa, while PAM was effective against 64%. Both phages remained stable at subinhibitory concentrations of cefepime and meropenem and when employed with sub-inhibitory concentrations of cefepime and meropenem, exhibited synergistic effects against planktonic bacterial cells and demonstrated efficacy in both biofilm inhibition and eradication.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"605"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}