BMC Microbiology最新文献

{"title":"Disease-resistant watermelon variety against Fusarium wilt by remodeling rhizosphere soil microenvironment.","authors":"Bingye Yang, Caipeng Yue, Chunhui Guo, Mingzi Zheng, Xiefeng Yao, Jinhua Xu, Sijie Huang, Mengmeng Yang","doi":"10.1186/s12866-025-04065-6","DOIUrl":"https://doi.org/10.1186/s12866-025-04065-6","url":null,"abstract":"<p><strong>Background: </strong>Rhizosphere microorganisms and their interactions play a critical role in enhancing plant disease resistance. Here, we found that the disease severity of the resistant variety LW025 showed a decreasing trend with the increase in continuous cropping cycles. However, the mechanisms underlying the reduction in disease severity during the continuous cropping of the resistant watermelon variety LW025, particularly its relationship with the rhizosphere microbiome, remain unclear.</p><p><strong>Results: </strong>In this study, the transcriptome of different watermelon varieties after continuous planting in pathogen-containing and pathogen-free soils was analyzed. The results showed that only two genes expression showed significant differences in disease-resistant variety between healthy and diseased soils. Subsequently, we analyzed the differences of rhizosphere soil microbial communities after planting different watermelon varieties for three consecutive seasons, as well as the relationship between differential microorganisms and soil physiochemical properties and soil enzyme activity. The results demonstrated continuous cropping of the disease-resistant variety LW025 formed a rhizosphere microbiome different from the initial soil and susceptible variety. Specifically, fungal changes were primarily observed in Ascomycota and Chytridiomycota, while bacterial changes were mainly observed in Cyanobacteria and Gemmatimonadetes. The bacterial functions enriched in the rhizosphere of the resistant variety LW025 after continuous cropping were primarily associated with soil nitrogen cycling. Furthermore, the plant disease index showed a significant positive correlation with the available phosphorus and potassium content in the soil, while exhibiting a significant negative correlation with soil pH and catalase activity.</p><p><strong>Conclusions: </strong>Overall, the reduction in disease severity associated with continuous cropping of the disease-resistant variety LW025 was more closely related to changes in the rhizosphere microecological environment. This study explained the mechanism of the resistant variety LW025 against Fusarium infection, and provided new prospects for the development of technologies based on rhizosphere microecological environment modification to improve the resistance of watermelon to Fusarium wilt.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"350"},"PeriodicalIF":4.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First detection of resistance genes and virulence factors in Escherichia coli and Salmonella spp in Togo: the case of imported chicken and frozen by-products.","authors":"Kossi Touglo, Bouraïma Djeri, Haziz Sina, Bawa Boya, Guy Géoffroy Ayadokoun, Lamine Baba-Moussa, Yaovi Ameyapoh","doi":"10.1186/s12866-025-04076-3","DOIUrl":"10.1186/s12866-025-04076-3","url":null,"abstract":"<p><strong>Background: </strong>The increasing importation of frozen poultry into Togo raises concerns about the microbiological safety and antimicrobial resistance of associated pathogens. Despite the public health risks posed by resistant foodborne bacteria, data on resistance profiles, resistance genes, and virulence factors in imported frozen chickens in Togo remain limited. This study aims to address this gap by characterizing these factors in pathogenic strains isolated from imported poultry.</p><p><strong>Methods: </strong>A cross-sectional prospective study was undertaken to assess the microbiological quality and resistance profiles of imported poultry products. Samples were collected from seven cold storage facilities located within the Golfe prefecture of the Greater Lomé metropolitan area. In total, 285 poultry meat and cut samples were analyzed following standardized AFNOR microbiological protocols. Isolated Salmonella spp. and Escherichia coli strains underwent antibiotic susceptibility testing using the disk diffusion method, adhering to the guidelines established by the Comité de l'Antibiogramme de la Société Française de Microbiologie (CA-SFM). Furthermore, polymerase chain reaction (PCR) assays were employed to identify genetic determinants of antibiotic resistance and virulence factors in the bacterial isolates.</p><p><strong>Results: </strong>Microbiological analysis revealed a prevalence of Escherichia coli of 32.98%, while Salmonella spp. were detected in 2.46% of the samples. Antibiotic susceptibility testing demonstrated resistance among isolates to several beta-lactams and quinolones. Specifically, resistance to cefoxitin was observed in 14.28% of strains, whereas resistance to cefalexin, cefuroxime, ceftazidime, ceftriaxone, and nalidixic acid was uniformly detected at a prevalence of 28.57%. Among the E. coli isolates, 9.44% exhibited multidrug resistance to both beta-lactams and quinolones. Molecular characterization identified class 1 integrons in 17.6% of isolates, with gene cassettes predominantly harboring aadA1 and dfr1, which encode resistance to streptomycin, spectinomycin, and trimethoprim. Notably, class 2 and class 3 integrons were absent. Additionally, the plasmid-mediated qnrB gene was detected in 5.9% of isolates. The study also documented the emergence of resistance to third-generation cephalosporins (C3G), primarily associated with extended-spectrum beta-lactamase (ESBL) production, as evidenced by the presence of blaCTX (35.3%) and blaTEM (58.8%) genes among ESBL-producing strains.</p><p><strong>Conclusions: </strong>This study reveals a notable presence of antimicrobial-resistant Escherichia coli and Salmonella in imported frozen poultry in Togo, highlighting significant public health risks. The findings call for improved surveillance and stricter control measures to prevent the spread of resistant pathogens via the food supply.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"349"},"PeriodicalIF":4.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Candida albicans cells exhibit media specific proteomic profiles during induction of filamentation.","authors":"Véronique Legros, Véronique Albanese, Guillaume Chevreux, Jean-Michel Camadro, Françoise Auchère","doi":"10.1186/s12866-025-04080-7","DOIUrl":"10.1186/s12866-025-04080-7","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"348"},"PeriodicalIF":4.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12128542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of antimicrobial resistance, integrons and virulence genes in Escherichia coli isolated from drinking water in Van, Turkiye: a cross-sectional study.","authors":"Erva Rakici, Elif Aydin, Elif Seren Tanriverdi, Osman Birol Ozgumus","doi":"10.1186/s12866-025-04046-9","DOIUrl":"10.1186/s12866-025-04046-9","url":null,"abstract":"<p><strong>Background: </strong>Escherichia coli is one of the recommended freshwater indicator organisms for faecal contamination and can be associated with the pool of genes encoding antibiotic resistance. The aim of this study was to investigate the molecular characterisation of antibiotic resistance and to identify the virulence genes in E. coli isolated from drinking water samples collected for the purpose of monitoring microbial drinking water quality.</p><p><strong>Methods: </strong>The E. coli isolates (n = 244) from municipal tap water samples collected in Van province in Turkiye over a five-month period in 2018 were examined in this study. Antimicrobial susceptibility testing was performed using the standard disc diffusion method with 12 antibiotics. Integron content, the resistance genes such as bla_TEM, bla_SHV, bla_CTX-M groups, tetA-E genes, qnrA, qnrB, qnrS, qepA, and aac(6')-Ib-cr were screened and the virulence genes (afa, sfa, fimA, hly, iroN, aer, cnf, iutA and pap) were assessed by specific PCRs. The fingerprinting of the isolates harbouring the integron was evaluated using dendogram analysis of the band patterns obtained from the enterobacterial repetitive intergenic consensus (ERIC) PCR.</p><p><strong>Results: </strong>Ampicillin, tetracycline, streptomycin, nalidixic acid and trimethoprim/sulfamethoxazole were the most common resistances. MAR index of 18.03% of the isolates was greater than 0.2. The two isolates produced ESBL. Thirty seven (15.16%) isolates carried the class 1 or class 2 integrons harboured the gene cassettes such as aadA, sat, estX and unnamed protein gene. Thirty two (21.47%) isolates were found to carry bla_TEM, 5 (3.33%) isolates carried bla_SHV and 2 (1.3%) isolates carried the bla_CTX-M group genes. tetA (81.25%) and tetB (91.66%) were present in tetracycline-resistant isolates. qnrS (29.92%), qnrB and aac(6')-Ib-cr (3.84%) were present in the nalidixic acid resistant isolates. Most of the isolates carried the virulence genes such as fimA, iutA, iroN, aer and sfa. Isolates belonged to 65.57% B1, 15.98% A, 9.42% D and 9.01% B2 phylogroups. A statistically significant difference was found between the phylogenetic groups of the isolates and the integron content (p < 0.001). Fingerprinting showed the 25 different phylotypes in four clusters.</p><p><strong>Conclusions: </strong>Molecular epidemiological findings in such a five-month period in the 11 sampled districts in the city of Van appearently showed us that the tap waters in public use was continuously contaminated by the antibiotic resistant and virulent E. coli isolates. This could pose a serious public health risk and, thus need the serious precautions for the infrastructures.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"345"},"PeriodicalIF":4.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MtlA gene sequencing based on 3rd -generation sequencing for Vibrio typing.","authors":"Rensi Zhou, Yingju Qi, Lei Zhou, Danlei Liu, Yaozhong Zou, Ting Xian, Zilong Zhang, Huan Hu, Huajun Zheng","doi":"10.1186/s12866-025-04078-1","DOIUrl":"10.1186/s12866-025-04078-1","url":null,"abstract":"<p><strong>Background: </strong>Traditional serotyping and multilocus sequence typing (MLST) for Vibrio parahaemolyticus have limitations in resolution and accuracy, particularly for strains with close genetic relationships. The long read length and high sequencing quality of a new type of nanopore sequencer G-seq500 make it possible to type V. parahaemolyticus strains using mtlA, a new identified typing gene for Vibrio. This gene not only offers advantages in cost-effectiveness and speed relative to MLST but also demonstrates comparable resolution.</p><p><strong>Results: </strong>We sequenced the full-length mtlA gene (1,953 bp) of 33 V. parahaemolyticus strains using Geneus G-seq500. A total of 199,658 long reads with an average length of 17,306 bp were produced by one sequencing chip. After recognizing adaptor and barcode, each long read can be split into 12 subreads with mean accuracy of 93.25%, which were then aligned into 64,086 consensus sequences with a mean quality Q35 (sequence accuracy 99.88-99.93%). The final mtlA gene was generated by clustering all the consensus sequencing from same strain, and its accuracy reached 100% with over four consensus sequences clustered together. Then we developed a naming rule based on mtlA clustering of 7,573 mtlA sequences from NCBI together with the 33 mtlA gene sequenced by G-seq500, with the largest cluster composed of 2,321 strains designated as mtlA0001.</p><p><strong>Conclusions: </strong>Our study confirms the high sequencing accuracy of Geneus G-seq500, and the effectiveness of using the full-length mtlA gene for V. parahaemolyticus strain typing and epidemiological surveillance.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"347"},"PeriodicalIF":4.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated microbiome and metabolome analysis reveals a novel interplay between gut microbiota and metabolites in differentiated thyroid carcinoma.","authors":"Xue Jiang, Qian Liu, Dongkun Xu, Hua Pang, Yuhong Shi","doi":"10.1186/s12866-025-03877-w","DOIUrl":"10.1186/s12866-025-03877-w","url":null,"abstract":"<p><strong>Background: </strong>Differentiated Thyroid carcinoma (DTC) is the most prevalent endocrine malignancy. The identification of novel biomarkers for thyroid carcinoma is essential for enhancing our understanding of the molecular mechanisms underlying DTC development. Notably, gut microorganisms and their metabolites play a role in the development of DTC, although their influence is modulated by the host's genetic background and environmental factors. Our study aimed to identify and classify gut microbiota and metabolites associated with DTC.</p><p><strong>Methods: </strong>90 patients with a confirmed diagnosis of DTC and 33 healthy volunteers donated stool samples for our analysis. To examine the gut microbiota, we utilized 16 S rRNA gene sequencing, a technique that allows for the identification and classification of microorganisms. Additionally, we employed liquid chromatography-mass spectrometry (LC-MS) to investigate the alterations in metabolites present in thyroid carcinoma patients compared to healthy individuals.</p><p><strong>Results: </strong>The Venn diagram visualized the distribution of bacterial species, with 926 species shared by both groups and 12,225 species unique to DTC patients. Notably, the gut microbiota of DTC patients exhibited higher species richness and diversity compared to healthy individuals. LDA Effect Size (LEfSe) analysis identified Faecalibacterium and Prevotella_9 as more abundant in healthy individuals, while Oscillospiraceae, Subdoligranulum, and Actinobacteriota were significantly more prevalent in DTC patients. We successfully characterized 3255 metabolites in both groups, which were primarily associated with biosynthesis of plant secondary metabolites, neomycin, kanamycin, and gentamicin biosynthesis, bile secretion, and steroid hormone biosynthesis. Among these metabolites, 550 were differentially expressed, with 402 metabolites being highly expressed in DTC patients. Six metabolites exhibiting an area under the curve (AUC) value exceeding 0.87 were identified as potential clinical diagnostic markers for DTC. Furthermore, Spearman's rank correlations were utilized to explore the potential functional relationships between the 10 distinctive microbial species and the top 10 differential metabolites.</p><p><strong>Conclusions: </strong>The gut microbiota and its associated metabolites may play a crucial role in the development of DTC. The identification of altered metabolites and microbiota in DTC patients suggests their potential as diagnostic markers and therapeutic targets. This offers new insights into the molecular pathogenesis of DTC, providing opportunities for early diagnosis and improved treatment strategies.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"346"},"PeriodicalIF":4.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic, genomic, and ecological insights into a novel Flavobacteriaceae strain from coastal tidal flats.","authors":"Haohao Wang, Jiahui Liu, Yifan Guo, Yaqin Chen, Chi Zhang, Shan He, Weiyan Zhang, Lijian Ding","doi":"10.1186/s12866-025-04069-2","DOIUrl":"10.1186/s12866-025-04069-2","url":null,"abstract":"<p><strong>Background: </strong>Tidal flats are vital coastal ecosystems that play a significant role in organic carbon accumulation and biogeochemical cycles. Members of the family Flavobacteriaceae is known for its ability to degrade complex organic matter, including polysaccharides. However, the ecological roles and metabolic capabilities of Flavobacteriaceae in tidal flat environments remain underexplored.</p><p><strong>Results: </strong>In this study, we isolated and characterized a novel bacterium, strain NBU2967^T, from the tidal flats of Meishan Island in the East China Sea. Phylogenetic and genomic analyses identified this strain as a new genus and species within the family Flavobacteriaceae, for which we propose the name Meishania litoralis gen. nov., sp. nov. Comprehensive polyphasic characterization, including morphological, physiological, chemotaxonomic, and genomic analyses, confirmed its distinct taxonomic status. Genomic analysis revealed a diverse set of carbohydrate-active enzymes (CAZymes), along with multiple metabolic pathways associated with carbon and sulfur cycling, highlighting the strain's potential adaptation to organic-rich marine environments. Comparative genomic and pangenome analyses further demonstrated significant genetic divergence from related taxa. Environmental distribution data revealed that the newly proposed genus Meishania is widely distributed across global marine ecosystems.</p><p><strong>Conclusions: </strong>We isolated and characterized a novel bacterium, designated NBU2967^T (= KCTC 82912^T = MCCC 1K06391^T), for which we propose the name Meishania litoralis gen. nov., sp. nov. This strain is classified as a new genus within the family Flavobacteriaceae. The strain's ability to process both carbon and sulfur compounds underscores its ecological significance in marine ecosystems. These findings provide novel insights into the ecological functions of the family Flavobacteriaceae in coastal tidal flats environments and enhance our understanding of microbial-mediated degradation and transformation of chemical compounds in dynamic coastal ecosystems.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"344"},"PeriodicalIF":4.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12124053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the antimicrobial and anticancer potential of culturable fungal endophytes isolated from the stems of Kirkia acuminata Oliv.","authors":"Mfundo Magagula, Thabiso E Motaung, Zukile Mbita, Khumiso Dithebe","doi":"10.1186/s12866-025-03964-y","DOIUrl":"10.1186/s12866-025-03964-y","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"343"},"PeriodicalIF":4.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into Candida auris dispersal cells and their impact on antifungal resistance.","authors":"Bahgat Fayed","doi":"10.1186/s12866-025-04055-8","DOIUrl":"10.1186/s12866-025-04055-8","url":null,"abstract":"<p><p>The emerging Candidozyma auris (formerly known as Candida auris, C. auris) has caused several outbreaks globally. While several studies explored the resistant biofilm formed by C. auris, little is known regarding the cells dispersed following biofilm formation. Herein, I investigated and characterized the cells dispersed from C. auris biofilms. Cells dispersed from biofilm developed in 96 well plate were isolated and counted. The antifungal susceptibility testing showed that the dispersed cells display similar antifungal susceptibility as the parent planktonic cells, except amphotericin B. Gene expression analysis performed by quantitative real-time PCR indicated that dispersed cells can express genes coded for antifungal resistance (ERG2, ERG6, ERG11, FKS1, CHS1, CHS2, CDR1, MDR1) more than the parent planktonic cells. It was observed that dispersed cells can acquire resistance to caspofungin faster than the parent planktonic cells once exposed to caspofungin at sub MIC level. Furthermore, biofilms formed by dispersed cells displayed significantly higher metabolic activity, as indicated by the XTT analysis. To provide more insight, I explored the expression of genes coding for biofilm initiation and maturation and the data obtained indicated that dispersed cells overexpressed ALS5 and KRE6 genes. Further, GC-MS analysis indicated that dispersed cells exhibit altered metabolic profile that enhance cells survivability under stress and nutrient limit condition. The presented study is the first to explore C. auris dispersed cells and indicated that they are not able to revert to the planktonic mode once released from the biofilm.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"342"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12121289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic attributes, antioxidant and neuromodulatory effects of GABA-Producing Lactiplantibacillus plantarum SY1 and optimization of GABA production.","authors":"Xinfeng Bai, Pu Shi, Weihua Chu","doi":"10.1186/s12866-025-04070-9","DOIUrl":"10.1186/s12866-025-04070-9","url":null,"abstract":"<p><p>Γ-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the central nervous system, has been shown to alleviate various physiological disorders including insomnia, hypertension, depression, and memory loss. Lactic acid bacteria (LAB), recognized as safe GABA producers, have attracted increasing attention. This study aimed to screen GABA-producing LAB from naturally fermented dairy products and evaluate their probiotic potential, antioxidant and neuromodulatory activities, while optimizing GABA production. GABA-producing LAB were screened using the Berthelot method and thin-layer chromatography. The safety of Lactiplantibacillus plantarum SY1 was assessed through hemolysin production and drug sensitivity tests. L. plantarum SY1 demonstrated high tolerance to acidic conditions and low bile salt concentrations, along with significant antioxidant capacity (49 ± 0.2% DPPH radical scavenging rate, 86.1 ± 0.14% hydroxyl radical scavenging rate, and 32.7 ± 1.6% superoxide radical anion scavenging rate). In vivo experiments revealed that L. plantarum SY1 extended the lifespan of C. elegans N2, enhanced oxidative stress resistance, and delayed paralysis in transgenic C. elegans (CL4176) by 23.53%. Through OFAT strategy and RSM optimization, GABA production reached 1.49 g/L under optimal conditions (37℃, pH 4.44, 96 h fermentation, and 4.16% inoculum). These findings demonstrate that L. plantarum SY1 is a promising GABA-producing strain with antioxidant and neuromodulatory effects, suggesting its potential as an anti-aging and neuroprotective probiotic.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"341"},"PeriodicalIF":4.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12121261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}