BMC Microbiology最新文献

{"title":"Correction: Biological characterization and genomic analysis of the newly discovered mycobacteriophage WST1.","authors":"Xingyan Tan, Xiaorui Zhang, Zhiqun He, Zhiping Luo, Ni An, Kerui Ye, Donghui Ke, Yuqing Li, Jumei Zeng","doi":"10.1186/s12866-025-04365-x","DOIUrl":"https://doi.org/10.1186/s12866-025-04365-x","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"637"},"PeriodicalIF":4.2,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of multi-drug resistant and extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae among meat products sold at Sohag Governorate, Egypt.","authors":"Usama Hassan Abo-Shama, Aly El Sayed Abo-Amer, Eman Abd El-Atty Ahmed, Reem Mohamed Alsaadawy, Haitham Helmy Sayed","doi":"10.1186/s12866-025-04392-8","DOIUrl":"https://doi.org/10.1186/s12866-025-04392-8","url":null,"abstract":"<p><p>Meat products (MPs) are among the most commonly consumed food items in Egypt, and may serve as a potential vehicle for transmission of Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) to humans. This study aimed to determine the prevalence of E. coli and K. pneumoniae in MPs marketed in Sohag Governorate, Egypt, with a particular focus on extended-spectrum β-lactamases (ESBL)-producing and Shiga toxin-producing E. coli (STEC), as well as to evaluate their antimicrobial resistance (AMR) profiles. Therefore, a total of 150 MP samples (30 of each burger, kofta, luncheon, minced meat, and sausage) were randomly collected from the stores and supermarkets in Sohag Governorate and subjected to bacteriological examinations. E. coli and K. pneumoniae were detected in 10% and 4% of the samples, respectively. Among the isolates, 66.7% of both E. coli and K. pneumoniae were multi-drug resistant (MDR), and all the isolates had a multiple antibiotic resistance (MAR) index above 0.2. Moreover, 53.3% of E. coli and 33.3% of K. pneumoniae isolates were ESBL-producers, and demonstrated higher levels of co-resistance to non-β-lactam antibiotics, compared to non-ESBL-producers isolates. PCR analysis revealed the presence of resistance and virulence genes in the investigated E. coli isolates (n = 10), including bla_CTX-M, bla_TEM, dfrA, stx1, and stx2 genes, with 90%, 80%, 90%, 10%, and 20% prevalence, respectively. E. coli isolates carrying stx1 or stx2 gene were found as MDR and ESBL-producing isolates. The concordance between genotypic and phenotypic AMR ranged from 30% to 90% %, indicating varying degrees of correlation. The findings highlight the presence of MDR, ESBL-producing E. coli, K. pneumoniae, and STECs in retail MPs in Sohag Governorate, posing a potential risk to public health. These results underscore the urgent need for improved hygienic measures along the food production chain and stricter regulations on the use of antimicrobials in food animals.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"636"},"PeriodicalIF":4.2,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of MgO, ZnO, chitosan hydroxyapatite and silver hydroxyapatite nanoparticles against carbapenem-resistant Gram-negative Egyptian clinical isolates: a combined in-silico and in-vitro study.","authors":"Salma Jamal Ahmed, Shima Mahmoud Ali, Mohammed A Khedr, Mohamed Emara, Selwan Hamed","doi":"10.1186/s12866-025-04364-y","DOIUrl":"https://doi.org/10.1186/s12866-025-04364-y","url":null,"abstract":"","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"632"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Touching Surfaces - Presence of microorganisms on antimicrobial metal surfaces on the International Space Station and in German schools.","authors":"Carolin L Krämer, Daniel W Müller, Franca Arndt, Anna Rehm, Bernd Walkenfort, Aisha S Ahmed, Aaron Haben, Alessa Schiele, Alina Auerhammer, Mike Hasenberg, Alessa L Boschert, Ralf Kautenburger, Stefan Leuko, Stefan Janssen, Matthias Maurer, Frank Mücklich, Katharina Siems","doi":"10.1186/s12866-025-04316-6","DOIUrl":"https://doi.org/10.1186/s12866-025-04316-6","url":null,"abstract":"<p><p>Microorganisms are an integral component of human health on Earth as well as for life on the International Space Station. However, inescapably, fomites in human habitats can serve as crucial niches for opportunistic pathogens. To explore potential countermeasures for the associated infection risk, the Touching Surfaces experiment evaluated antibacterial surfaces as high-touch surfaces on the International Space Station and on Earth. We used copper-based surfaces that integrate chemical antimicrobial properties with topography, thereby creating a metasurface. 16 S rRNA sequencing revealed that most bacteria found were human associated. While no significant distinction was observed between the microbial communities on the reference and antibacterial surfaces, isolation of microorganisms from the surfaces suggests that copper-based nanometer-structured surfaces exhibit enhanced antibacterial efficacy. The antibacterial efficacy of touched surfaces was reduced, as assessed by wet contact killing assays carried out using a methicillin-resistant Staphylococcus aureus (MRSA) isolate. The simplicity of implementing the surfaces allowed for straightforward testing of surfaces in both space and on Earth.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"631"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity study of SARS-CoV-2 Spike protein expressed on Bacillus subtilis spores.","authors":"Atiqah Hazan, Amalia A Saperi, Nurfatihah Zulkifli, Nor-Aziyah Mat Rahim, Vunjia Tiong, Hai Yen Lee, Sazaly AbuBakar","doi":"10.1186/s12866-025-04186-y","DOIUrl":"https://doi.org/10.1186/s12866-025-04186-y","url":null,"abstract":"<p><strong>Background: </strong>The SARS-CoV-2 spike protein is the primary antigen used in most COVID-19 vaccines. To improve protection, especially at mucosal surfaces, a robust and safe delivery system capable of inducing both systemic and mucosal immunity is needed. Bacillus subtilis spores offer a promising platform due to their stability, safety, and capacity for protein display. A study to evaluate the potential acute oral toxicity of recombinant B. subtilis spores expressing SARS-CoV-2 spike proteins was undertaken.</p><p><strong>Results: </strong>B. subtilis spore was used to express the SARS-CoV-2 S1 domain spike proteins during the bacterial sporulation. No in vitro cytotoxicity was observed when the spores were used to treat cells. BALB/c mice were given a single oral dose (5 × 10¹¹ CFU/ml) over three consecutive days and monitored for 14 days to assess acute toxicity. BALB/c mice fed with up to 3 × 10¹¹ CFU/mouse of the spores showed no adverse effects. A gross necropsy and histology of the treated mice's vital organs, kidneys, liver, heart, lungs, and spleen revealed no pathological alterations, and the biochemical parameters remained within normal ranges.</p><p><strong>Conclusion: </strong>These findings suggest that the SARS-CoV-2 S1 domain spike proteins expressed on B. subtilis spores is well tolerated in mice when administered orally at a high dose. Findings from this study, hence, support further investigation of the recombinant bacterial spores expressing SARS-CoV-2 S1 domain spike proteins as an oral vaccine candidate against SARS-CoV-2 infection.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"633"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of compensatory mutations in rifampicin-resistant tuberculosis and their association with compensated transmission in Ningbo, China.","authors":"Yang Che, Xiangchen Li, Dongliang Zhang, Guoxin Sang, Junli Gao, Junshun Gao, Yewei Lu, Zhengwei Liu","doi":"10.1186/s12866-025-04390-w","DOIUrl":"https://doi.org/10.1186/s12866-025-04390-w","url":null,"abstract":"<p><strong>Background: </strong>The global spread of rifampicin-resistant tuberculosis (RR-TB) presents a significant challenge to tuberculosis control, with compensatory mutations hypothesized to offset the fitness cost of drug resistance, thereby facilitating transmission. However, the characteristics and epidemiological impact of these mutations in coastal regions of Eastern China remain inadequately understood. This study aimed to characterize the spectrum of compensatory mutations in RR-TB isolates and to assess their association with transmission dynamics in a well-developed coastal region of Eastern China.</p><p><strong>Methods: </strong>We collected RR-TB cases identified through drug-resistance surveillance in Ningbo, China, from 2021 to 2024. Whole-genome sequencing (WGS) was performed on 180 RR-TB isolates to identify resistance-conferring and compensatory mutations, particularly in the rpoA, rpoB, and rpoC genes. Transmission clusters were inferred using single nucleotide polymorphism (SNP) analysis, and the association between compensatory mutations and the risk of RR-TB clustering was evaluated using logistic regression.</p><p><strong>Results: </strong>Among the 180 RR-TB isolates analyzed, 28.9% harbored putative compensatory mutations, predominantly in rpoC. Isolates with compensatory mutations were significantly more likely to be part of genomic transmission clusters than those without such mutations (odds ratio: 4.28, 95% CI: 2.07-8.85). No significant differences in demographic or clinical characteristics were observed between clustered and non-clustered cases. Phylogenetic analysis indicated ongoing local transmission of compensated RR-TB strains.</p><p><strong>Conclusion: </strong>Compensatory mutations are prevalent among RR-TB strains in coastal Eastern China and are strongly associated with increased transmission, underscoring their role in sustaining the RR-TB epidemic in this region. Enhanced molecular surveillance and targeted interventions are warranted to curb the spread of compensated RR-TB.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"634"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical-mediated effects of Chaetoceros affinis on colony formation of Phaeocystis globosa: induced release of allelopathic compounds.","authors":"Dayong Liang, Huanxin Li, Hua Xiang","doi":"10.1186/s12866-025-04378-6","DOIUrl":"https://doi.org/10.1186/s12866-025-04378-6","url":null,"abstract":"<p><p>Phaeocystis globosa is a significant cause of harmful algal blooms in temperate and tropical regions, with outbreaks typically occurring after spring diatom blooms. A previous study revealed that Chaetoceros affinis promotes the formation of P. globosa colonies. However, the composition of its extracellular metabolites and the underlying molecular mechanisms driving colony formation remain unclear. This study utilized C. affinis and its filtrate as stress conditions to stimulate P. globosa. Results indicated that both C. affinis and its filtrate induced a life-history transition in P. globosa. The abundance of solitary cells decreased, whereas colony abundance, colony diameter, and the number of colonial cells increased. Additionally, the filtrate induced significant alterations in the metabolic pathways of P. globosa cells. Photosynthesis was enhanced; genes related to DNA replication were downregulated, while those involved in fatty acid synthesis, amino acid synthesis, and polysaccharide synthesis were upregulated, and its degradation were downregulated. The reduced concentrations of 3-hydroxy-4-methoxycinnamic acid, abietic acid, 4,6-dinitro-o-cresol, and oleamide in the filtrate favored algal growth, thereby promoting colony formation. Nonetheless, the specific metabolites responsible for colony formation warrant further characterization in future studies. This study offers new insights into the role of chemical interactions in regulating the alternation processes between diatoms and P. globosa.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"635"},"PeriodicalIF":4.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and genomic evaluation of novel exopolysaccharide produced by marine Pediococcus pentosaceus E3 with antidiabetic, anticancer, and anti-inflammatory potentials.","authors":"Nancy M El Halfawy, Eman H Zaghloul","doi":"10.1186/s12866-025-04370-0","DOIUrl":"10.1186/s12866-025-04370-0","url":null,"abstract":"<p><p>Lactic acid bacteria (LAB) exopolysaccharides (EPS) are highly valuable due to their unique structure and functional properties. Pediococcus pentosaceus E3 is a promising marine probiotic strain. An investigation of the E3 genome identified a gene cluster responsible for EPS production, comprising 13 genes organized into four regions: the regulatory region for EPS expression, the chain length determination region, genes that catalyze the biosynthesis of EPS repeat units, and genes for polymerization and EPS transportation. Furthermore, a total of 16 key enzymes involved in the nucleotide sugar biosynthesis pathway were predicted according to the KEGG metabolic pathways in the E3 genome sequence. Therefore, the current study investigates the characteristics and bioactivities of E3-EPS. E3 strain was grown in MRS broth supplied with 1.0% sucrose for EPS production, and E3 produced a significant quantity of EPS (400 mg/L). Structural characteristics of E3-EPS were investigated through carbohydrate content determination, FTIR, SEM, EDX, TGA, HPLC, and NMR. HPLC analysis revealed that E3-EPS is a heteropolysaccharide composed of four sugar moieties: galactose, glucose, mannose, and fucose. Moreover, E3-EPS demonstrated promising bioactivities, as its anticancer activity was evaluated against colon cancer cell lines, and the IC₅₀ value was determined to be 77.05 ± 0.24 µg/mL. E3-EPS inhibited α-amylase activity by 58.3% and 82.8% at 10 and 100 µg/mL concentrations, respectively. Additionally, E3-EPS successfully decreases the expression levels of inflammatory cytokines (TNF-α and IL-6). The findings of this study suggest that the safe marine probiotic P. pentosaceus E3 is a source of unique EPS suitable for pharmacological applications.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"628"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of probiotic yeasts from traditional fermented horsemeat products in Xinjiang: functional evaluation and potential application.","authors":"Xue Sun, Yuxian Wang, Dilmurat Zilale, Tong Shen","doi":"10.1186/s12866-025-04322-8","DOIUrl":"10.1186/s12866-025-04322-8","url":null,"abstract":"<p><p>Traditional fermented horsemeat and horsemeat sausages in Xinjiang are rich in microbial resources, mainly including lactic acid bacteria and yeasts. Although the probiotic effects of lactic acid bacteria have been widely studied, there is relatively little research on the probiotic properties of yeasts, which limits their application in functional food development. This study isolated 44 yeast strains from traditional fermented horsemeat products in Xinjiang, and 16 strains exhibiting strong tolerance to temperature, acidic pH, simulated gastrointestinal fluids, and high bile salt solution were screened. Molecular identification revealed that these strains belonged to Candida zeylanoides (C-5, C-8, C-13, C-15, C-21, C-22, C-24, R-8), Candida parapsilosis (R-10, R-12, R-14, R-19, R-20), Candida metapsilosis (R-18), and Rhodotorula alborubescens (R-6, R-11). Several strains demonstrated significantly higher cell surface hydrophobicity, auto-aggregation, and co-aggregation with pathogenic bacteria than the control strain Saccharomyces boulardii CNCM I-745, indicating strong intestinal adhesion and antagonistic potential. All strains have high antioxidant activity (> 60%) and certain cholesterol-lowering ability. Furthermore, extracellular metabolites from select strains exhibited antibacterial activity and potential glucose-lowering effects. Specifically, C. zeylanoides C-8 inhibited α-glucosidase by 54.14%, and C-8 inhibited angiotensin-converting enzyme (ACE) by 37.05%. Hemolysis assays confirmed that all strains were non-hemolytic, suggesting good biosafety. These findings highlight the multi-functional probiotic potential of yeast strains from traditional fermented horsemeat products and provide a scientific basis for their development as novel functional food ingredients.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"630"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12495793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptomyces zaomycetitus strain GH90: a source of violet pigment with metabolic profiling and potential application in textile: in vitro supported by in silico studies and molecular docking.","authors":"Gehad H El Sayed, Mohamed Fadel, Mohamed Marzouk, Hend M Ahmed, Nervana S Diab, Ahmed A Hamed","doi":"10.1186/s12866-025-04329-1","DOIUrl":"10.1186/s12866-025-04329-1","url":null,"abstract":"<p><p>Dyes are used daily in the culinary, textile, paper, leather, and agricultural sectors. Synthetic colors pollute the environment and have harmful effects. Microorganism-produced pigments are more cost-effective and environmentally benign than animal, plant, and synthetic pigments. The present study focuses on isolating a pigment-producing actinomycete, a novel violet pigment produced by a local isolate strain identified as S. zaomycetitus GH90, using 16 S rRNA from Egyptian soil. Optimizing violet pigment yield necessitated the precise adjustment of various growth parameters. Streptomyces zaomyceticus GH90 achieved optimal production under specific conditions. pH 8.0, agitation at 180 rpm, temperature at 37 °C, 1.5% starch concentration, ammonium sulfate, dipotassium hydrogen phosphate, 2% inoculum, 20% medium volume, and a 10-day incubation period. Ethanol served as the solvent for pigment extraction. Thermal analysis indicated no degradation at 40 °C. At 50 °C, retention was 98.7% and remained stable at 97.5% when subjected to temperatures between 60 and 80 °C for 1 h. Retention remained above 91% at temperatures of 90 and 100 °C, indicating significant heat stability. The pigment preserved its color integrity in an aqueous solution at pH 8.0. The UV-Vis spectra exhibited a maximum wavelength (λmax) at 580 nm. LC-MS analysis revealed the presence of indole, scopoletin, myricetin, and phosphorylated compounds, which are likely contributors to color and bioactivity. The pigment exhibited superior textile dyeing characteristics, including vivid coloration, high durability, and significant antibacterial activity, establishing it as a versatile and effective dye for antimicrobial textiles. Moreover, the most prevalent molecule, has high permeability, moderate solubility, and selective enzyme interactions, making it a suitable therapeutic candidate. Molecular docking shows significant OMPA and OprD binding affinities, supported by hydrophobic, π-π stacking, and hydrogen bond interactions. The obtained results clearly show how important Streptomyces is as a source of bioactive metabolites with potential applications.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"627"},"PeriodicalIF":4.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}