BMC Microbiology最新文献

{"title":"Inhibition of Enterotoxigenic Escherichia coli adhesion via aptamers prevents infection in IPEC-J2 cells.","authors":"Zefeng Wang, Tao Xu, Jianxi Tan, Yan Zhou, Qiyi Li, Zhihui Peng","doi":"10.1186/s12866-025-04233-8","DOIUrl":"https://doi.org/10.1186/s12866-025-04233-8","url":null,"abstract":"<p><p>Enterotoxigenic Escherichia coli (ETEC) is a well-known strain associated with post-weaning diarrhea. Consequently, strategies to prevent and inhibit ETEC infections are critically important. Aptamers are single-stranded DNA or RNA sequences that exhibit high affinity and specificity for binding to target molecules. In this study, we used the intestinal porcine epithelial cell line IPEC-J2 as an in vitro model to demonstrate that two aptamers, K88-Apt A04 (a fimbrial-specific aptamer targeting ETEC K88) and K88-Apt 37 (a cell-specific aptamer targeting ETEC K88) effectively inhibited the adhesion of ETEC K88 to intestinal epithelial cells. We established an ETEC K88-IPEC-J2 interaction model by exposing the IPEC-J2 cell line to ETEC K88 at a multiplicity of infection of 10. Our findings revealed that the aptamers inhibited ETEC K88 adhesion to IPEC-J2 cells in a concentration-dependent manner, primarily through adherence inhibition and prevention. Furthermore, enzyme-linked immunosorbent assay and mRNA expression analyses indicated that the aptamers reduced the secretion of pro-inflammatory cytokines triggered by ETEC K88 infection. In conclusion the aptamers demonstrated the ability to reduce ETEC K88-induced cytotoxicity in IPEC-J2 cells in vitro.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"546"},"PeriodicalIF":4.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis and optimization of polyhydroxyalkanoates production by environmental bacterial isolates from Nepal.","authors":"Sandesh Adhikari, Saman Bhattarai, Roja Shrestha, Shikha Shree Shah, Usha Kunwar","doi":"10.1186/s12866-025-04314-8","DOIUrl":"https://doi.org/10.1186/s12866-025-04314-8","url":null,"abstract":"<p><p>The versatile non-biodegradable material plastic has significantly enhanced innovation, but its production heavily relies on fossil fuels and non-renewable resources, which causes severe pollution and ecosystem disruption, highlighting the urgent need for eco-friendly alternatives. Polyhydroxyalkanoates (PHA) are a class of bioplastics that offer a promising solution as biodegradable, environmentally compatible, and versatile biopolymer synthesized by microorganisms using various substrates, aiding in organic waste management. This study was aimed to isolate and profile PHA producing bacteria from diverse sources such as soil, compost, landfill site, and sewage in Nepal. The initial screening of bacteria was performed by Sudan Black B dye, followed by secondary screening with the more specific Nile Blue A dye to detect PHA accumulation. Out of 343 isolates, 81 were confirmed positive for PHA production which were further processed for PHA extraction. The isolates Ht3d (12.76 ± 1.854%), Nk3e (22.748 ± 3.608%), Mn7d (14.24 ± 2.223%), and Dg5c (14.952 ± 3.401%) from soil, compost, landfill, and sewage respectively, showed the highest PHA accumulation and were biochemically identified as Bacillus circulans, Bacillus subtilis, Staphylococcus aureus, Staphylococcus spp., respectively. The yield ratio for isolate Ht3d significantly increased by 2.74 fold under the optimal conditions (pH 7, 35 °C, and 48 h with glucose as carbon source) which was 34.99 ± 5.61% having titer value of 0.82 g/L and production rate of 0.034 g/L/h with respect to 2.4 g/L of cell biomass. The production rate and dry cell weight of other isolates were also enhanced under different optimal conditions. The characterization of the produced biopolymer through UV-Visible spectrophotometry provided maximum absorbance at 232 nm and the FT-IR spectroscopy indicated the presence of O-H and C-H vibrations along with C = O, C-O-C stretching which collectively confirmed the presence of PHA.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"548"},"PeriodicalIF":4.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predicted sorting platform dynamically associates with the type III secretion system from Xanthomonas euvesicatoria in response to the external pH.","authors":"Christian Otten, Daniela Büttner","doi":"10.1186/s12866-025-04227-6","DOIUrl":"https://doi.org/10.1186/s12866-025-04227-6","url":null,"abstract":"<p><p>The Gram-negative bacterium Xanthomonas euvesicatoria (Xe) causes bacterial spot disease in pepper and tomato plants, and employs a type III secretion (T3S) system to translocate effector proteins into plant cells. The T3S system is a highly complex nanomachine which spans both bacterial membranes and is conserved in different bacterial species. In animal-pathogenic bacteria, structural and functional studies showed that the membrane-spanning secretion apparatus dynamically associates with a cytoplasmic sorting platform for substrate docking. The sorting platform is a wheel-like structure and contains the central ATPase SctN, which is connected via SctL to peripheral pod-like SctQ complexes. The architecture and function of a similar substructure of the T3S system in plant-pathogenic bacteria is still unknown. We previously reported that the SctQ homolog HrcQ from Xe forms complexes with its alternative translation initiation product HrcQ_C, which stabilizes HrcQ and contributes to T3S. In the present study, we analysed the protein-protein interaction network of predicted sorting platform components from Xe and performed localization studies with HrcQ and the ATPase HrcN by fluorescence microscopy. We show that HrcQ complex formation depends on the SctL homolog HrcL which interacts with HrcQ and HrcN via its N- and C-terminal regions, respectively. Both HrcQ and HrcL contribute to HrcN stability, suggesting that a preassembled HrcQ-HrcL complex associates with and stabilizes the ATPase. Colocalization studies revealed that the HrcN-HrcL-HrcQ complex preferentially assembles at the membranes under T3S-permissive conditions when bacteria are cultivated at a low external pH. In contrast, at pH 7.0, HrcN and HrcQ form large cytoplasmic aggregates. Our data indicate that the interaction of the predicted sorting platform with the T3S system is influenced by external pH levels. These dynamic protein-protein interactions likely control the initiation of effector delivery in response to environmental stimuli, a strategy that might be also used by other plant pathogens.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"545"},"PeriodicalIF":4.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shallow shotgun metagenomic sequencing of vaginal microbiomes with the Oxford Nanopore technology enables the reliable determination of vaginal community state types and broad community structures.","authors":"Enid Graeber, Alona Tysha, Azlan Nisar, Daniel Wind, Werner Mendling, Patrick Finzer, Alexander Dilthey","doi":"10.1186/s12866-025-04236-5","DOIUrl":"https://doi.org/10.1186/s12866-025-04236-5","url":null,"abstract":"<p><strong>Background: </strong>The vaginal microbiome plays an important role in female health; it is associated with reproductive success, susceptibility to sexually transmitted infections, and, importantly, the most prevalent vaginal condition in reproduction-age women, bacterial vaginosis (BV). Traditionally, 16S rRNA gene sequencing-based approaches have been used to characterize the composition of vaginal microbiomes, but shallow shotgun metagenomic sequencing (SMS) approaches, in particular when implemented with the Oxford Nanopore Technologies, have important potential advantages with respect to cost effectiveness, speed of data generation, and the availability of flexible multiplexing schemes.</p><p><strong>Results: </strong>Based on a study cohort of n = 52 women, of which 23 were diagnosed with BV, we evaluated the applicability of Nanopore-based SMS for the characterization of vaginal microbiomes in direct comparison to Illumina 16S-based sequencing. We observed perfect agreement between the two approaches with respect to detecting the dominance of individual samples by either Lactobacilli, vaginosis-associated, or other taxa; very high concordance (92%) with respect to community state type (CST) classification; and a high degree of concordance with respect to the overall clustering structures of the sequenced microbiomes. Comparing the inferred abundances of individual species in individual samples, we observed significant differences (Wilcoxon signed-rank test p < 0.05) between the two approaches for 12 of the 20 species most abundant in our cohort, indicating differences in the fine-scale characterization of vaginal microbiomes. Higher overall abundance of Gardnerella vaginalis, associated with an increased number of CST IV detections, in the Nanopore shallow SMS data indicated potentially increased sensitivity of this approach to dysbiotic states of the vaginal microbiome. Nanopore shallow SMS also enabled the methylation-based quantification of different human cell types in the characterized samples as well as the detection of non-prokaryotic species, including Lactobacillus phage and Candida albicans in study participants with microscopically detected Candida. One important potential limitation of the evaluated Nanopore-based SMS approach was marked variation in sequencing yields.</p><p><strong>Conclusion: </strong>Our study demonstrated the successful application and potential advantages of Nanopore-based shallow SMS for the characterization of vaginal microbiomes and paves the way for its application in larger-scale research or diagnostic settings.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"544"},"PeriodicalIF":4.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From sequence to activity: the HgaI-homologous restriction modification system RM.MhoVI of Mycoplasma hominis.","authors":"Lars Vogelgsang, Manuel Dolgopolow-Schmidt, Azlan Nisar, Dana Bäcker, Alexander T Dilthey, Birgit Henrich","doi":"10.1186/s12866-025-04270-3","DOIUrl":"https://doi.org/10.1186/s12866-025-04270-3","url":null,"abstract":"<p><p>Restriction-modification (RM) systems are widespread defense mechanisms in prokaryotes that protect the host from potentially harmful foreign DNA. They typically consist of a DNA methyltransferase (MTase), which methylates the host genome at an adenine (6 mA methylation) or cytosine (4mC or 5mC), and a restriction endonuclease (REase), which cleaves foreign, unmethylated DNA. In addition to the 2023 published family of 5mC-MTases, an HgaI-homolog RM system was detected in Mycoplasma hominis with the more rare constellation of two 5mC MTase genes, called RM.MhoVI. A qPCR screening of 239 randomly selected M. hominis isolates revealed a prevalence of the MhoVI-RM system of 12.97% (n = 31/239). Notably, in all tested MhoVI-positive isolates, the MhoVI-RM cassette localized between MHO_3110 and MHO_3120 and comprised an XRE-family transcriptional regulator gene in addition to the RM genes. Intra-species conservation of the encoded MhoVI-enzymes was high (> 99% identities), and inter-species conservation was the lowest compared to the eponymous species Haemophilus gallinarum (46.6% M1.MhoVI; 48.1% M2.MhoVI; 27.4% R.MhoVI). A polycistronic organization of the MhoVI-genes was strongly suspected due to the discovery of gene-overlapping mRNA regions. The MTases activity was demonstrated in RM.MhoVI positive M. hominis isolates by protection of genomic DNA from cleavage by the methylation-sensitive endonuclease HgaI; and bioinformatics analysis using the Dorado basecaller on the Oxford Nanopore sequenced genomes revealed methylation rates of the respective motifs, 5'-GA^mCGC-3'/5'-G^mCGTC-3', above 95% in MhoVI-positives, with a higher methylation frequency of 5'-GA^mCGC-3' than 5'-G^mCGTC-3 in most isolates. A final proof of MhoVI-RM representing an HgaI-RM-like methylation activity was demonstrated through expression and analysis of recombinant rM2.MhoVI in E. coli.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"543"},"PeriodicalIF":4.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12376442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reverse vaccinology-based identification and in silico characterization of immunogenic membrane proteins of Salmonella Typhimurium as novel vaccine targets against multidrug-resistant infections.","authors":"Tooba Ume Habiba, Zahid Hussain, Fatima Asghar, Wajeeha Nawaz","doi":"10.1186/s12866-025-04124-y","DOIUrl":"https://doi.org/10.1186/s12866-025-04124-y","url":null,"abstract":"<p><strong>Background: </strong>Salmonella enterica serovar Typhimurium (S. Typhimurium) is a leading cause of salmonellosis, gastroenteritis, sepsis, and reactive arthritis. Transmission primarily occurs through contaminated water, eggs, meat, and dairy products. The disease disproportionately affects developing nations, where young children, the elderly, and immunocompromised individuals face high risks of severe morbidity and mortality. Its ability to evade host immune defenses and acquire multidrug resistance (MDR) exacerbates global public health challenges. Currently, no licensed human vaccine is available, underscoring the urgent need for targeted vaccine development.</p><p><strong>Methods: </strong>This study utilized a reverse vaccinology approach and in silico strategies to identify highly immunogenic membrane proteins as potential vaccine candidates. The complete proteome of S. Typhimurium was screened for membrane-associated candidates using the SOSUI server. Antigenicity was evaluated using VaxiJen v2.0 (threshold ≥ 0.9), and allergenicity was assessed using AllerTOP v1.1. To ensure vaccine safety, homologous proteins were excluded based on PSI-BLAST analysis against the human proteome, and toxicity was predicted using ToxinPred. The immunogenic potential was further evaluated through C-ImmSim immune simulation software. B-cell and T-cell epitopes were predicted using ABCpred and the Immune Epitope Database (IEDB). Physicochemical characteristics were analyzed with ProtParam and TMHMM 2.0. Finally, BLASTp analysis was used to confirm the conservation of the selected proteins across MDR clinical isolates.</p><p><strong>Results: </strong>Nine membrane proteins were prioritized based on strong antigenicity, non-allergenicity, non-toxicity, favorable epitope profiles, and physicochemical stability. All proteins were highly conserved in MDR isolates, supporting their utility for broad-spectrum vaccine development.</p><p><strong>Conclusion: </strong>These targets show promising potential for developing a broadly protective multi-epitope vaccine against S. Typhimurium. However, in vitro and in vivo experimental validation is essential to confirm their immunogenicity and protective efficacy.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"542"},"PeriodicalIF":4.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of gut and lung microbiota in patients with lung masses and their relationship with clinical features.","authors":"Yanping Yang, Jiacheng Shen, Sulan Wei, Maosong Ye, Xing Zhao, Jian Zhou, Lin Tong, Jie Hu, Yuanlin Song, Shengdi Wu, Nuo Xu","doi":"10.1186/s12866-025-04325-5","DOIUrl":"https://doi.org/10.1186/s12866-025-04325-5","url":null,"abstract":"<p><strong>Objectives: </strong>The exploration of how dysbiosis relates to lung masses is still nascent, with few studies focusing on the microbial characteristics across various sites. Therefore, we categorized the microbiota into feces and bronchoalveolar fluid (BALF) groups to compare microbial characteristics between benign and malignant masses, analyze their clinical correlations, and develop predictive models for lung cancer.</p><p><strong>Methods: </strong>A total of 238 fecal samples and 34 BALF samples were collected from patients with benign and malignant masses and then analyzed by 16 SrRNA. We explored the distinct composition of the gut and lung microbiota and their associations with clinical features. The diagnostic models were constructed using microbial features identified through two approaches: random forest algorithm with five-fold cross-validation and comparative analysis of significantly differential taxa. The performance evaluation was subsequently conducted using receiver operating characteristic (ROC) analysis.</p><p><strong>Results: </strong>There was no significant difference in α-and β-diversity between feces and BALF groups. The relative abundance of Lachnospiraceae_NK4A136_group (P = 0.003232) and Erysipelotrichaceae_UCG-003 (P = 0.01316) in feces group and Proteobacteria (P = 0.03654) in BALF group were significantly increased in lung cancer patients. We also found Bacteroides (P = 0.01458) was abundant in NSCLC than those of SCLC in feces group, while the BALF group was dominated by norank_c_Cyanobacteria (P = 0.03384). Smoking history appeared to be related to the distribution of microbiota, with enrichment of Parabacteroides (P = 0.02054) in feces and Prevotella_1 (P = 0.03286) in BALF. Furthermore, the patients with Sellimonas (P = 0.04148) in feces and Alloprevotella (P = 0.04283) in BALF seemed to have better response to chemotherapy combined with immunotherapy. For differentiating benign and malignant masses, the combination of Megasphaera and norank_p__Saccharibacteria in BALF demonstrated superior predictive performance, with an AUC reaching 0.8 (95% CI 0.59-1).</p><p><strong>Conclusion: </strong>The microbiota composition significantly differed between benign and malignant masses in both fecal and BALF groups, with minimal evidence supporting microbial migration between these two sites. Our findings suggest that BALF microbiota may serve as a more reliable biomarker for lung masses classification, offering valuable insights for early diagnosis and clinical decision-making.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"541"},"PeriodicalIF":4.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the aerobic stability, antioxidant and microbial community of Broussonetia papyrifera ensiled with ferulic acid esterase-producing Lactiplantibacillus plantarum in combination with cellulase and/or xylanase.","authors":"YiXi Long, Mengxin Li, Ya Su, Qiang Yu, Yuanjiang Rong, Yulong Xi, Hong Sun, Yixiao Xie, Jun Hao, Chao Chen, Yulong Zheng, Fuyu Yang","doi":"10.1186/s12866-025-04185-z","DOIUrl":"https://doi.org/10.1186/s12866-025-04185-z","url":null,"abstract":"<p><strong>Background: </strong>Ferulic acid possesses certain antioxidant and antibacterial properties. Additionally, ferulic acid esterase (FAE) and cellulolytic enzymes have been associated with synergistic degradation of ferulic acid ester bonds, thereby facilitating greater release of ferulic acid from lignocellulose, which could have important effects on silage quality and aerobic stability.</p><p><strong>Methods: </strong>This study examined the effects of ensiling Broussonetia papyrifera with FAE-producing Lactiplantibacillus plantarum (LP), cellulase (CE) and xylanase (XY) under aerobic exposure conditions. The following treatments were used: distilled water (CK), LP, LP + CE, LP + XY and LP + XY + CE. After 60 days of silage treatment, the samples were unsealed for aerobic exposure for 1, 3, 5, or 7 days.</p><p><strong>Results: </strong>Compared with the CK treatment, the addition of FAE-producing L. plantarum significantly (P < 0.05) led to lower pH, reduced dry matter loss of the silage and increased lactic acid (LA) concentration after 60 d of ensiling (especially for the LP + CE and LP + CE + XY groups). During the aerobic exposure stage, the combined treatment with LP and enzymes effectively inhibited the increase in pH, significantly reduced the rate of dry matter loss and increased the LA concentration and aerobic stability of the silage (P < 0.05). Moreover, the LP + CE and LP + CE + XY treatment groups exhibited higher ferulic acid levels than the other groups did, corresponding with greater aerobic stability, especially for the LP + CE group, which remained stable. In this group, the pH values showed minimal change, increasing by only 0.31 (4.24-4.55) after 7 days of aerobic exposure. In addition, the LP and enzyme co-treatment was linked to shifts in the microbial community of the silage during aerobic exposure, with increased relative abundance of Lactiplantibacillus plantarum, and its abundance positively correlated with lactic acid and ferulic acid concentrations, while negatively correlated with ammonia nitrogen; and inhibited proliferation of spoilage-related bacteria (Enterobacter, Gluconobacter and Cladosporium).</p><p><strong>Conclusions: </strong>The combination of FAE-producing L. plantarum and cellulase can be used as an effective method to increase the preservation efficiency and aerobic stability of B. papyrifera silage.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"533"},"PeriodicalIF":4.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology and genomic features of Bordetella pertussis in Tianjin, China, 2023.","authors":"Xiaohui Lu, Wen Li, Xuan Chen, Wei Zhang, Aiping Yu, Guangwen Liu, Baolu Zheng, Xin Gao, Yamin Liu, Xiaoyan Li","doi":"10.1186/s12866-025-04276-x","DOIUrl":"https://doi.org/10.1186/s12866-025-04276-x","url":null,"abstract":"<p><strong>Background: </strong>Pertussis, a highly contagious respiratory disease caused by Bordetella pertussis (BP), poses challenges due to genomic variation that may contribute to its persistence in vaccinated populations and the development of antimicrobial resistance.</p><p><strong>Methods: </strong>In this study, we characterized 27 BP isolates collected from Tianjin, China in 2023, using whole-genome sequencing and multilocus genotyping (MGT) to explore their genomic diversity. For comparative analysis, we selected 165 international BP genomes to examine the genomic diversity and evolutionary traits of Tianjin strains within a global context.</p><p><strong>Results: </strong>The results revealed that all 27 isolates belonged to ST2 (100.0%), with no distinct phylogenetic clustering observed. Furthermore, phylogenetic analysis integrating 165 global BP genomes indicated that the Tianjin strains exhibited high genomic homogeneity with globally circulating strains. Specifically, 16 isolates (59.3%) carried the virulence genotype ptxA-1/ptxC-4/ptxP-3/prn-150(truncated)/fim2-1/fim3-1/fhaB-1, while 11 isolates (40.7%) carried the genotype ptxA-1/ptxC-4/ptxP-3/prn150/fim2-1/fim3-1/fhaB-1. Additionally, all 27 isolates exhibited complete resistance to erythromycin, azithromycin, clarithromycin, and clindamycin, whereas 18.5% of them were resistant to levofloxacin.</p><p><strong>Conclusions: </strong>This study elucidates the phylogenetic, genomic characteristics, and drug sensitivity profiles of BP strains isolated in Tianjin, China, in 2023, providing valuable insights into BP persistence in vaccinated populations.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"534"},"PeriodicalIF":4.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the new antimicrobial benziothiazolinone for management of fire blight disease of pear.","authors":"Jiuxiang Zhu, Ling Cai, Yixin Wang, Wenxi Jiang, Xinran Liu, Jun Wang, Yanli Tian, Baishi Hu, Youfu Zhao","doi":"10.1186/s12866-025-04238-3","DOIUrl":"https://doi.org/10.1186/s12866-025-04238-3","url":null,"abstract":"<p><strong>Background: </strong>Fire blight, caused by Erwinia amylovora, is a devastating disease that affects apples and pears, leading to significant economic losses worldwide. The disease is typically managed through a combination of biological, cultural, and chemical strategies. Due to the emergence of antibiotic resistance and concerns regarding chemical residues, there is an urgent need for new bactericides that are both highly effective and low in toxicity. Recently, the Ministry of Agriculture and Rural Affairs of China approved the registration of a new bactericide, benziothiazolinone also known as 1,2-Benzisothiazol-3-one.</p><p><strong>Results: </strong>The assessment of benziothiazolinone demonstrated an EC₅₀ value of 0.48 µg/mL against E. amylovora BZ16 after a 10-hour inoculation period, and 0.67 µg/mL after 16 h. Furthermore, benziothiazolinone displays a broad antimicrobial spectrum against various microorganisms, including bacteria, fungi, and oomycetes, as evidenced by its EC₅₀ values. Prolonged exposure to benziothiazolinone was found to enhance the production of amylovoran in the E. amylovora strain BZ16^BITR; however, this treatment significantly diminished the pathogenicity of the strain. Resistance risk analysis indicates that E. amylovora has a low risk of developing resistance to benziothiazolinone. Results from two-year field trials demonstrated that benziothiazolinone could significantly reduce the disease incidence of fire blight. Compared to the streptomycin control, the application of benziothiazolinone exhibited excellent efficacy, achieving over 80% effectiveness against the disease.</p><p><strong>Conclusion: </strong>These findings indicate that benziothiazolinone is a promising agrochemical for the management of fire blight of pear.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"540"},"PeriodicalIF":4.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}