Molecular epidemiology and genomic features of Bordetella pertussis in Tianjin, China, 2023.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

BMC Microbiology Pub Date : 2025-08-23 DOI:10.1186/s12866-025-04276-x

Xiaohui Lu, Wen Li, Xuan Chen, Wei Zhang, Aiping Yu, Guangwen Liu, Baolu Zheng, Xin Gao, Yamin Liu, Xiaoyan Li

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Abstract

Background: Pertussis, a highly contagious respiratory disease caused by Bordetella pertussis (BP), poses challenges due to genomic variation that may contribute to its persistence in vaccinated populations and the development of antimicrobial resistance.

Methods: In this study, we characterized 27 BP isolates collected from Tianjin, China in 2023, using whole-genome sequencing and multilocus genotyping (MGT) to explore their genomic diversity. For comparative analysis, we selected 165 international BP genomes to examine the genomic diversity and evolutionary traits of Tianjin strains within a global context.

Results: The results revealed that all 27 isolates belonged to ST2 (100.0%), with no distinct phylogenetic clustering observed. Furthermore, phylogenetic analysis integrating 165 global BP genomes indicated that the Tianjin strains exhibited high genomic homogeneity with globally circulating strains. Specifically, 16 isolates (59.3%) carried the virulence genotype ptxA-1/ptxC-4/ptxP-3/prn-150(truncated)/fim2-1/fim3-1/fhaB-1, while 11 isolates (40.7%) carried the genotype ptxA-1/ptxC-4/ptxP-3/prn150/fim2-1/fim3-1/fhaB-1. Additionally, all 27 isolates exhibited complete resistance to erythromycin, azithromycin, clarithromycin, and clindamycin, whereas 18.5% of them were resistant to levofloxacin.

Conclusions: This study elucidates the phylogenetic, genomic characteristics, and drug sensitivity profiles of BP strains isolated in Tianjin, China, in 2023, providing valuable insights into BP persistence in vaccinated populations.

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2023年中国天津地区百日咳博德泰拉分子流行病学及基因组特征

背景：百日咳是一种由百日咳博德泰拉（BP）引起的高度传染性呼吸道疾病，由于基因组变异可能导致其在接种人群中的持续存在和抗菌素耐药性的发展，因此面临挑战。方法：利用全基因组测序和多位点基因分型（MGT）技术，对2023年在中国天津采集的27株BP分离株进行基因组多样性分析。为了进行比较分析，我们选择了165个国际BP基因组，在全球范围内研究天津菌株的基因组多样性和进化特征。结果：27株分离株均为ST2型（100.0%），未见明显的系统发育聚类。此外，整合165个全球BP基因组的系统发育分析表明，天津菌株与全球流行菌株具有高度的基因组同源性。其中16株（59.3%）携带ptxA-1/ptxC-4/ptxP-3/prn-150（截短）/fim2-1/fim3-1/fhaB-1基因型，11株（40.7%）携带ptxA-1/ptxC-4/ptxP-3/prn150/fim2-1/fim3-1/fhaB-1基因型。此外，27株分离菌株对红霉素、阿奇霉素、克拉霉素和克林霉素均表现出完全耐药，而对左氧氟沙星的耐药率为18.5%。结论：本研究阐明了2023年在中国天津分离的BP菌株的系统发育、基因组特征和药物敏感性，为BP在接种人群中的持久性提供了有价值的见解。

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.