BMC Microbiology最新文献

{"title":"Diversity of sulfur cycling halophiles within the Salton Sea, California's largest lake.","authors":"Linton Freund, Caroline Hung, Talyssa M Topacio, Charles Diamond, Alyson Fresquez, Timothy W Lyons, Emma L Aronson","doi":"10.1186/s12866-025-03839-2","DOIUrl":"10.1186/s12866-025-03839-2","url":null,"abstract":"<p><strong>Background: </strong>Microorganisms are the biotic foundation for nutrient cycling across ecosystems, and their assembly is often based on the nutrient availability of their environment. Though previous research has explored the seasonal lake turnover and geochemical cycling within the Salton Sea, California's largest lake, the microbial community of this declining ecosystem has been largely overlooked. We collected seawater from a single location within the Salton Sea at 0 m, 3 m, 4 m, 5 m, 7 m, 9 m, 10 m, and 10.5 m depths in August 2021, December 2021, and April 2022.</p><p><strong>Results: </strong>We observed that the water column microbiome significantly varied by season (R² = 0.59, P = 0.003). Temperature (R² = 0.27, P = 0.004), dissolved organic matter (R² = 0.13, P = 0.004), and dissolved oxygen (R² = 0.089, P = 0.004) were significant drivers of seasonal changes in microbial composition. In addition, several halophilic mixotrophs and other extremotolerant bacteria were consistently identified in samples across depths and time points, though their relative abundances fluctuated by season. We found that while sulfur cycling genes were present in all metagenomes, their relative coverages fluctuated by pathway and season throughout the water column. Sulfur oxidation and incomplete sulfur oxidation pathways were conserved in the microbiome across seasons.</p><p><strong>Conclusions: </strong>Our work demonstrates that the microbiome within the Salton Seawater has the capacity to metabolize sulfur species and utilize multiple trophic strategies, such as alternating between chemorganotrophy and chemolithoautrophy, to survive this harsh, fluctuating environment. Together, these results suggest that the Salton Sea microbiome is integral in the geochemical cycling of this ever-changing ecosystem and thus contributes to the seasonal dynamics of the Salton Sea. Further work is required to understand how these environmental bacteria are implicated relationship between the Salton Sea's sulfur cycle, dust proliferation, and respiratory distress experienced by the local population.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"120"},"PeriodicalIF":4.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical, coagulation, and platelet count profiles among Schistosoma mansoni infected patients attending at selected Dembiya health institutions, Northwest Ethiopia.","authors":"Wagaw Abebe, Wossenseged Lemma, Yalewayker Tegegne, Assefa Sisay, Tadesse Misganaw, Sisay Ayana, Desie Kasew, Marye Nigatie Debash, Alemu Birara Zemariam, Tadele Emagneneh, Adane Derso","doi":"10.1186/s12866-025-03838-3","DOIUrl":"10.1186/s12866-025-03838-3","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Schistosomiasis is a parasitic disease that causes coagulation disorders and biochemical abnormalities. This is due to liver failure, platelet destruction, disruption of blood flow, and endothelial function by the schistosomes. However, there is no adequate data on biochemical and coagulation profiles and platelet count of patients infected with Schistosoma mansoni in Dembiya Selected Health Institutions. Hence, the aim of this study was to assess the effect of Schistosoma mansoni infection on selected biochemical and coagulation profiles and platelet count.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Method: &lt;/strong&gt;An institutional-based comparative cross-sectional study was conducted from March to August 2022 at Dembiya Primary Hospital, Chuahit Health Center, and Abrija Health Center, Northwest Ethiopia. A total of 70 individuals were enrolled in the study using convenient sampling techniques. A stool sample was collected for Schistosoma mansoni detection. Likewise, a blood sample was collected for biochemical and coagulation profiles and platelet count analysis. The data were analyzed using SPSS version 25. A p-value less than 0.05 was considered statistically significant.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Median values for alanine aminotransferase, aspartate aminotransferase, creatinine, total bilirubin, and direct bilirubin values were significantly higher, while total protein and glucose were significantly lower in Schistosoma mansoni infected than in the healthy control participants (P &lt; 0.05). Prothrombin time, activated partial thromboplastin time, and international normalization ratio were significantly higher, while the platelet count was significantly lower in the Schistosoma mansoni infected than healthy control participants (P &lt; 0.05). The values of alanine aminotransferase, aspartate aminotransferase, creatinine, total bilirubin, direct bilirubin, prothrombin time, activated partial thromboplastin time, and international normalization ratio were significantly higher, while total protein, glucose, and platelet count were significantly lower in those with moderate and heavy Schistosoma mansoni infection intensity compared to healthy control participants (P &lt; 0.05). The number of Schistosoma mansoni eggs per gram of stool had a positive correlation with biochemical and coagulation profiles, except for total protein, glucose, and platelet count, which were correlated negatively in Schistosoma mansoni infected participants (P &lt; 0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Biochemical and coagulation profiles, including alanine aminotransferase, aspartate aminotransferase, creatinine, total bilirubin, direct bilirubin, glucose, total protein, prothrombin time, activated partial thromboplastin time, international normalization ratio, and platelet count, were significantly altered in S. mansoni infected participants compared to controls (p &lt; 0.05). These findings underscore the need for routine biochemical and coagulation monitoring in end","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"119"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved protocols for isolation of Mycobacterium ulcerans from clinical samples.","authors":"Bernadette Agbavor, Rejoice Agyeiwaa Arthur, Abigail Agbanyo, Dzifa Kofi Ahiatrogah, Charity Wiafe Akenten, Cynthia Adu-Asiamah, Kabiru Mohammed Abass, Elizabeth Ofori, George Amofa, Kwadwo Boampong, Thorsten Thye, Denise Dekker, Mathew Glover Addo, Mark Wansbrough-Jones, Tim John Bull, Yaw Ampem Amoako, Richard Odame Phillips","doi":"10.1186/s12866-025-03835-6","DOIUrl":"10.1186/s12866-025-03835-6","url":null,"abstract":"<p><strong>Background: </strong>The isolation and culture of Mycobacterium ulcerans (Mu) as a primary diagnostic modality for Buruli ulcer (BU) disease are limiting due to their low sensitivity and slow-growing nature. M. ulcerans cultures can also be overgrown with other bacteria and fungi. Culture, however, remains an important tool for the study of persisting viable M. ulcerans, drug susceptibility tests, and other molecular assays to improve management of the disease. The challenge of contamination with other fast-growing bacteria necessitates decontamination of clinical samples prior to culturing, but current methods may be too harsh, resulting in low yields of M. ulcerans. We aimed to evaluate a Tika-Kic decontamination process for M. ulcerans that uses supplements to stimulate M. ulcerans growth to improve recovery.</p><p><strong>Methods: </strong>Swab and Fine Needle Aspirate (FNA) samples were collected from 21 individuals with confirmed BU at baseline (week 0) and weeks 2 and 4 after initiating antibiotic treatment. Samples were decontaminated with Tika-Kic decontamination medium and the modified Petroff (NaOH) methods then inoculated each into Mycobacterium Growth Indicator Tube (MGIT) or Löwenstein Jensen (LJ) medium. Time to growth detection and confirmation by qPCR as well as the proportion of positive cultures for all three methods and the proportion of positive cultures for all three time points were documented. Common contaminating bacteria were also isolated and identified.</p><p><strong>Results: </strong>The proportion of M. ulcerans positive cultures obtained was higher for Tika-MGIT samples [14/43 (32%)] compared to Petroff-MGIT samples [10/43 (23%)] and Petroff-LJ samples [8/43 (19%)]. Baseline samples had a higher isolate proportion [17 (53%)] compared to samples collected after treatment initiation [9 (28%) for week 2 and 6 (19%) for week 4]. Contaminating bacteria isolated include Burkholderia cepacia, Pseudomonas aeruginosa, Pasteurella pneumotropica, Proteus mirabilis, Morganella morganii, Staphylococcus aureus and Enterococcus.</p><p><strong>Conclusion: </strong>Our study shows an advantage for culturing Mycobacterium ulcerans from clinical samples using the Tika-Kic decontamination and growth medium. Further research is needed to refine sample processing to improve M. ulcerans recovery.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"118"},"PeriodicalIF":4.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative in silico and in vivo study of the antioxidant activity of lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis against Rotavirus infection in male mice.","authors":"Tarek A M Ismail, Naglaa Elshafey, Shehab E Talat, Mona E Saif, Amany Mohammed Hegab, Komla Mawunyo Dossouvi, Hanan M Alharbi, Amr Elkelish, Khalid Abd El Ghany","doi":"10.1186/s12866-025-03820-z","DOIUrl":"10.1186/s12866-025-03820-z","url":null,"abstract":"<p><p>Rotavirus is a major cause of pediatric gastroenteritis, for which effective treatments are limited. This study investigates the antioxidant and antiviral potential of lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis against Rotavirus infection. In this study, Geobacillus stearothermophilus and Lactobacillus delbrueckii subsp. lactis were isolated from Hammam Pharon soil and milk cheese, respectively, and identified using molecular techniques with accession numbers PP758390 and PP758383. The antioxidant effect against DPPH showed that lactoferrin exhibited the strongest scavenging ability, followed by Geobacillus stearothermophilus and Lactobacillus delbrueckii subsp. lactis. In vivo experiments involved administering lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis in the drinking water of young mice for three days, followed by Rotavirus infection on the fourth day and sacrifice on the fifth day. The results demonstrated that lactoferrin significantly reduced the pathogenic effects of Rotavirus, as indicated by the normalization of inflammatory cytokines (TNF-α and IL-6) in the serum (p ≤ 0.001). Histological examination of small intestinal sections from Rotavirus-infected mice revealed extensive destruction of villus structures, while mice treated with lactoferrin showed no pathological changes compared to the control group. Geobacillus stearothermophilus-treated mice exhibited less pathological alteration and Lactobacillus delbrueckii subsp. lactis-treated mice showed mild pathological changes. Additionally, molecular docking studies indicated that bacteriocin (a bacterial protein) exhibited the highest binding affinity for the Rotavirus outer membrane protein (VP6) at -261.92 kcal/mol, outperforming lactoferrin (-229.32 kcal/mol). Additionally, bacteriocin's active compounds, turimicin (-7.9 kcal/mol) and lactin (-6.5 kcal/mol), also showed strong binding to VP6, suggesting their potential as therapeutic agents against Rotavirus. In conclusion, this study highlights the significant antiviral potential of lactoferrin against Rotavirus, demonstrating its ability to mitigate pathological changes and normalize inflammatory responses in infected mice. The findings also suggest that bacteriocins, particularly those with high binding affinities to Rotavirus proteins, could serve as promising candidates for therapeutic interventions against Rotavirus infections.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"117"},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11877866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and photoinactivation evaluation of zinc hydroxide chloride nanosheets against Streptococcus mutans.","authors":"Shima Afrasiabi, Fatemeh Saghatchi, Ahmad Reza Dehpour, Ramin Goudarzi, Mohammad Reza Karimi, Alireza Partoazar","doi":"10.1186/s12866-025-03822-x","DOIUrl":"10.1186/s12866-025-03822-x","url":null,"abstract":"<p><strong>Background: </strong>Considering the efficacy of antimicrobial photodynamic therapy (PDT) in inactivating bacteria, this study reports that zinc hydroxide chloride nanosheets (ZHC-NSs) are useful for this purpose.</p><p><strong>Materials & methods: </strong>The characterization of ZHC-NSs was performed using microscopic and spectroscopic techniques. The irritation test, acute toxicity test, and genotoxicity test of ZHC-NSs were evaluated and their effects on human pulp fibroblast cells (HPFC) viability, intracellular reactive oxygen species (ROS) levels, and antibacterial activity of ZHC-NSs (1-8 mg ml^-1) alone or in light conditions were investigated.</p><p><strong>Results: </strong>The ZHC-NSs structure showed a crystalline form and their sheets' thickness had an average size of 129.6 ± 19.50 nm. ZHC-NSs did not severely damage internal organs and were not genotoxic. The cytotoxic effect of ZHC-NSs on HPFC was concentration-dependent so that ZHC-NSs at higher concentrations (4 and 8 mg ml^-1) killed half of the HPFC cells. When ZHC-NSs were used in combination with a 980 nm diode laser, namely ZHC-NS^©, ROS production increased and led to enhanced antibacterial activity against Streptococcus mutans in planktonic and biofilm form. A statistically significant difference was found between ZHC-NSs without laser irradiation and photoexcited ZHC-NSs.</p><p><strong>Conclusion: </strong>ZHC-NSs^© with the potential ability to produce ROS could be effective in complementary treatment against S. mutans.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"116"},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11877797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated microbiome and metabolome analysis reveals altered gut microbial communities and metabolite profiles in dairy cows with subclinical mastitis.","authors":"Jie Yu, Chenhui Liu, Dingfa Wang, Pingmin Wan, Lei Cheng, Xianghua Yan","doi":"10.1186/s12866-025-03810-1","DOIUrl":"10.1186/s12866-025-03810-1","url":null,"abstract":"<p><strong>Background: </strong>Dairy cow mastitis is a common and prevalent disease arose by various complicated pathogeny, which poses serious threat to the health of cows, safety of dairy product and economic benefits for pastures. Due to the high stealthiness and long incubation period, subclinical mastitis (SM) of cows causes enormous economic losses. Besides the infection by exogenous pathogenic microorganisms, previous studies demonstrated that gastrointestinal microbial dysbiosis is one of the crucial causes for occurrence and development of mastitis based on the theory of entero-mammary axis. Whereas, limited researches have been conducted on potential pathological metabolic mechanisms underlying the relationship between gut microbiota and SM in cows.</p><p><strong>Results: </strong>The differences in blood parameters, gut microbiome, plasma and fecal metabolome between healthy and SM cows were compared by performing 16 S rDNA sequencing and non-targeted metabolomic analysis in the current study. The content of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and activity of catalase (CAT), total antioxidant capacity(T-AOC) were significantly decreased, while malondialdehyde (MDA) concentration was dramatically increased in serum of SM cows in comparison with healthy cows. The gut of cows with SM harbored more abundant Cyanobacteria, Proteobacteria, Succinivibrio and Lactobacillus_iners. Moreover, the abundance of Paraprevotella, Coprococcus, Succiniclasticum, Desulfovibrio and Bifidobacterium_pseudolongum were observably reduced in the gut of SM cows. Furthermore, higher abundance of pro-inflammatory metabolites were observed in feces (9(S)-HPODE, 25-hydroxycholesterol, dodecanedioic acid, etc.) and plasma (9-hydroxy-10,12-octadecadienoic acid, 13,14-dihydro PGF1α, 5,6-dehydro arachidonic acid, myristic acid, histamine, etc.) of SM cows. The abundance of certain metabolites with anti-inflammatory and antioxidant properties (mandelic acid, gamma-tocotrienol, deoxycholic acid, etc.) were notably decreased in feces or plasma of cows with SM.</p><p><strong>Conclusions: </strong>The intestinal microbial composition and metabolic profiles of healthy and SM cows were significantly distinct, that were characterized by decreased abundance of intestinal symbiotic bacteria, potential probiotics and anti-inflammatory, antioxidant compounds, along with increased abundance of potential pro-inflammatory bacteria, lipid metabolites, and the occurrence of oxidative stress in cows suffered from SM. The results of this study further enriched our understanding of the correlations between gut microbiota and metabolic profiles and SM, which provided insight into the formulation of management strategies for SM in cows.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"115"},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11877966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of rumen methane production and microbial community to different abatement strategies in yaks.","authors":"Qian Zhang, Tongqing Guo, Xungang Wang, Lin Wei, Yalin Wang, Shanshan Li, Hongjin Liu, Na Zhao, Shixiao Xu","doi":"10.1186/s12866-025-03817-8","DOIUrl":"10.1186/s12866-025-03817-8","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Developing region-specific dietary strategies is crucial for mitigating methane (CH&lt;sub&gt;4&lt;/sub&gt;) emissions from yaks. However, there is a lack of tailored emission reduction strategies for yak production in the Qinghai-Tibet Plateau region. This study utilizes an in vitro rumen fermentation technique (Based on the ANKOMRF gas production measurement system) to investigate the effects of different dietary interventions on CH&lt;sub&gt;4&lt;/sub&gt; production from regional yaks. The selected strategies-Sodium Nitrate solution, regional Medicago sativa L., and regional Helianthus tuberosus L.-were chosen for their potential to reduce CH&lt;sub&gt;4&lt;/sub&gt; production through various mechanisms: Sodium Nitrate as a methanogenesis inhibitor, Medicago sativa L. for its high nutritional value and its ability to modulate microbial fermentation, and Helianthus tuberosus L. due to its inulin content, which promotes beneficial microbial activity. These dietary interventions aim not only to reduce CH&lt;sub&gt;4&lt;/sub&gt; production but also to support rumen health and productivity. In addition, gas chromatography and microbial sequencing techniques were employed to identify the optimal emission reduction strategy for regional yaks and to elucidate the key factors influencing the efficacy of these strategies.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The results indicate that supplementing the confined feeding ration (FR group) with Sodium Nitrate (12 mmol/L, FRN group), Medicago sativa L. (25%, FRM group), and Helianthus tuberosus L. (3%, FRH group) all have the effect of reducing CH&lt;sub&gt;4&lt;/sub&gt; production from yak rumen. Among these interventions, the FRM group exhibits the most significant reduction, with a decrease in rumen CH&lt;sub&gt;4&lt;/sub&gt; production by 42.76% compared to the FR group. The dry matter digestibility, total volatile fatty acids (TVFA), propionate, and butyrate levels in all groups were higher than those in the FR group. However, only the FRM group reached a significant level (P &lt; 0.01). The pH values were significantly lower than those in the FR group (P &lt; 0.01) across all groups. Each group exhibited distinct clustering patterns in bacterial and archaeal communities compared to the FR group (P &lt; 0.05). The α diversity of bacterial communities was significantly lower than that of the FR group (P &lt; 0.01), while the α diversity of archaeal communities was significantly higher than that of the FR group (P &lt; 0.01). Taxa such as Lachnospiraceae, Clostridium, Treponema, Methanomicrobiaceae, Methanosphaera, and Methanoplanus were enriched in the FR group.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;CH&lt;sub&gt;4&lt;/sub&gt; production from yak rumen were significantly negatively correlated with substrate crude protein (CP) levels, fermentation fluid TVFA levels, α diversity of archaeal communities, and the relative abundance of Selenomonas and Megasphaera in bacterial communities (P &lt; 0.01). Conversely, CH&lt;sub&gt;4&lt;/sub&gt; production were significantly positively correlated with ","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"111"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The high efficiency protective effectiveness of a newly isolated myoviruses bacteriophage vB_AceP_PAc in protecting mice from Aeromonas caviae infection in mice.","authors":"Chao Feng, Lu Wang, Huifang Bai, Qixing Huang, Shuang Liang, Ruiqi Liang, Jiahao Yu, Shun Wang, Hui Guo, Sayed Haidar Abbas Raza, Xiaofeng Shan, Dongxing Zhang, Wuwen Sun, Lei Zhang","doi":"10.1186/s12866-025-03796-w","DOIUrl":"10.1186/s12866-025-03796-w","url":null,"abstract":"<p><p>Phage therapy is an effective strategy to combat multidrug resistance in bacteria and has been increasingly utilized to protect animals from bacterial infections. This study involved the isolation and purification of a novel myoviruses phage, vB_AceP_PAc, from a drug-resistant bacterial strain Aeromonas caviae. Genome sequence analysis based on nucleotide sequences revealed that vB_AceP_PAc shared significant similarity with the genomes of 10 other Aeromonas phages, with the highest coverage rate of 52% with phiA047. Intraperitoneal injection of 1 × 10⁷ CFU/mL (100 µL/mouse) A. caviae AC-CY resulted in diarrhea in mice three days later. At this time, oral administration of 1 × 10⁹ PFU/mL (100 µL/mouse) vB_AceP_PAc effectively alleviated the diarrhea induced by Pseudomonas aeruginosa infection in the mice. Furthermore, oral administration of 1 × 10⁹ PFU/ml vB_AceP_PAc (100 µl/mouse) in healthy mice significantly reduced inflammatory cytokine levels, increased tight junction molecule levels, and improved intestinal barrier function. Moreover, the consumption of vB_AceP_PAc by health mice led to a significant increase in the abundance of Lactobacillaceae in the gut, while the expression of CD3⁺CD4⁺/CD3⁺CD8⁺ was minimally affected. Overall, the findings of this study confirmed the promising potential of bacteriophage vB_AceP_PAc in treating diarrhea caused by A. caviae.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"112"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system.","authors":"Dandan Li, Wenxing Xu, Shengnan Huang, Wei An, Zhe Chao, Fang Zhao, Jun Ai, Junxing Yang, Shenyang Gao, Yuying Li, Lijun Chen, Guofeng Xu","doi":"10.1186/s12866-025-03807-w","DOIUrl":"10.1186/s12866-025-03807-w","url":null,"abstract":"<p><strong>Background: </strong>Brucella spp. are Gram-negative bacteria causing brucellosis, a major zoonotic disease affecting animals and humans. Annually, over 500,000 human cases are reported globally, with many undiagnosed due to nonspecific symptoms and diagnostic challenges. Current methods for Brucella detection, such as culture and serology, are time-consuming and lack specificity, hindering effective disease control. This study aims to develop a novel dual priming oligonucleotide (DPO) system-based PCR method for the specific detection of Brucella spp. in slaughter-aged livestock. This approach provides a rapid, sensitive, and field-deployable tool to improve early diagnosis and control of brucellosis.</p><p><strong>Methods: </strong>We developed a DPO system-based PCR assay for the specific detection of Brucella spp. in slaughter-aged livestock. The method utilizes two sets of primers designed to specifically target unique regions of the Brucella genome. The assay was validated for specificity using a panel of 15 non-target bacterial species commonly found in livestock, including Escherichia coli, Salmonella spp., and Campylobacter spp. Sensitivity was evaluated using DNA extracted from a range of Brucella strains, with detection limits assessed using serially diluted samples. The assay's performance was further tested on 500 samples from slaughter-aged sheep to assess its applicability in field conditions.</p><p><strong>Results: </strong>The DPO-based PCR assay demonstrated excellent specificity, with no cross-reactivity observed in any of the 15 non-target bacterial species tested. The assay was able to detect Brucella spp. at low DNA concentrations, with a sensitivity limit of approximately 5.3 × 10¹ CFU/mL of the Brucella per reaction. In the field validation, 500 samples from slaughter-aged sheep were tested, and the assay successfully identified Brucella infections in animals with no false positives or negatives. When compared to conventional PCR, the DPO-PCR method exhibited improved specificity and faster results, with a significantly reduced time to diagnosis.</p><p><strong>Conclusions: </strong>The DPO-based PCR assay provides a highly specific, rapid, and cost-effective tool for the detection of Brucella spp. in slaughter-aged livestock. This method is suitable for routine surveillance in slaughterhouses, offering a promising solution for early detection and control of brucellosis in both livestock and public health contexts. The assay's simplicity and robustness make it an ideal candidate for field deployment, particularly in resource-limited settings where timely disease control is crucial.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"110"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the fibrinolytic potential of marine Actinoalloteichus caeruleus isolated from Bay of Bengal coast.","authors":"Kothari Neeti Suresh, Subathra Devi C","doi":"10.1186/s12866-025-03815-w","DOIUrl":"10.1186/s12866-025-03815-w","url":null,"abstract":"<p><strong>Background: </strong>One of the main causes of several cardiovascular diseases that have an elevated mortality rate globally is intravascular thrombosis. The current fibrinolytic enzymes, are quite expensive and have a lot of side effects, thus it is necessary to develop alternate, economical techniques for the low-cost manufacture of these vital enzymes. Microbial fibrinolytic enzymes have the capacity to break up these clots and are relatively cheaper with minimal side effects and quick growth rate. Marine actinomycetota are the most prolific prokaryotes, which are capable of synthesizing novel secondary metabolites and are of industrial importance in pharmaceutical and various other industries. Thus, the objective of the research is to isolate, screen and characterize fibrinolytic protease producing actinomycetota from marine samples.</p><p><strong>Results: </strong>In this study, 35 actinomycetota have been successfully isolated from marine water and sediment samples. Among them, 12 isolates were protease positive and on secondary screening 5 isolates showed fibrinolytic activity. Out of the 5 isolates, one potent isolate's clot lysis activity was found out to be 93.12 ± 0.18% and its fibrinolytic potential was determined on fibrin agar plates. Based on the morphological, physiological, biochemical, and molecular analysis, the potent strain (NK60) was identified as Actinoalloteichus caeruleus.</p><p><strong>Conclusions: </strong>In this present study, a rare actinomycetota has been isolated from the Bay of Bengal coast. This is the first study reporting the potent fibrinolytic activity of A. caeruleus, isolated from marine water. This clot-busting enzyme has significant pharmacological value in the management of coronary artery diseases. In the near future, A. caeruleus can serve as an explicit source for commercial production of fibrinolytic enzymes.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"113"},"PeriodicalIF":4.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}