BMC Microbiology最新文献

{"title":"The effect of sample type and location on industrial workplace sink and hand dryer microbiomes.","authors":"T P Thompson, C J Rice, E Athanasakis, J Mawhinney, B F Gilmore, P Fitzgerald, T Skvortsov, S A Kelly","doi":"10.1186/s12866-025-04054-9","DOIUrl":"10.1186/s12866-025-04054-9","url":null,"abstract":"<p><p>One major issue in tackling antimicrobial resistance (AMR) is the ability to effectively track resistance spread in environments where surveillance is limited. Such environments include those experiencing high volumes of hand washing and drying from multiple users. This study characterised the microbial populations and antimicrobial resistomes of two different sample types from a pharmaceutical industrial site as part of an AMR environmental surveillance programme. Paired samples were collected from hand dryers and adjacent sinks in distinct sampling locations: from toilets adjacent to 'wet' labs, and locations associated with 'dry' activities. Microbial populations in hand dryers were significantly different to those of sinks, whereas there was no significant difference based on sample location. The opposite effect was observed for resistomes, where profiles differed significantly based on sample location, but not sample type. When both sample type and location were considered together, differences in microbiomes were driven primarily by hand dryer profiles from different locations. Analysis of metagenomically-assembled genomes revealed the presence of many poorly characterised organisms, and suggested no specific families predominated in terms of ARG carriage. This study emphasises the impact of human activities in determining the resistome of commonly used appliances, and the need for continued AMR surveillance programmes.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"325"},"PeriodicalIF":4.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of freezing period and meat juice model type on the survival, hydrophobicity, and acid resistance of Listeria monocytogenes and Salmonella Typhimurium.","authors":"Ata Kaboudari, Javad Aliakbarlu","doi":"10.1186/s12866-025-04039-8","DOIUrl":"10.1186/s12866-025-04039-8","url":null,"abstract":"<p><strong>Background: </strong>The survival of food-borne pathogenic bacteria in food processing environments and increasing their resistance to antimicrobials is one of the most crucial challenges in food safety. The present study aimed to investigate the effects of freezing period (0-60 days) and meat juice model (beef, mutton, and goat juices) on the survival, hydrophobicity, and acid resistance of Listeria monocytogenes and Salmonella Typhimurium.</p><p><strong>Methods: </strong>The survival of L. monocytogenes and S. Typhimurium in different meat juice models during the freezing period (0, 1, 2, 3, 4, 7, 14, 21, 30, and 60 days) was determined by colony counting method. The cell surface hydrophobicity of the bacteria was measured by bacterial adhesion to hydrocarbon assay. Changes in the bacterial resistance to acetic acid and lactic acid were evaluated by determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).</p><p><strong>Results: </strong>The survival of both bacteria was significantly higher in mutton juice than in other juices (p < 0.05). The survival rate of L. monocytogenes in freezing conditions was higher than that of S. Typhimurium. The amount of hydrophobicity was increased during the freezing period, and the highest increase was observed in the mutton juice. The increase in hydrophobicity of S. Typhimurium was higher than L. monocytogenes. The acid resistance of both bacteria, especially L. monocytogenes, was increased with increasing freezing time.</p><p><strong>Conclusion: </strong>Freezing stress and meat juice model type could change the survival, hydrophobicity, and acid resistance of L. monocytogenes and S. Typhimurium. The mutton juice model showed the highest protective effect on both bacteria during the freezing period. Meanwhile, the bacteria inoculated in the mutton juice model exhibited significantly higher hydrophobicity than those in other meat juices. The acid resistance of L. monocytogenes was significantly increased, especially in the mutton juice model, at the end of the freezing period.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"321"},"PeriodicalIF":4.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myochromella unveiled: exploring its global distribution through a public database of amplicons.","authors":"Shuwei Wei, Bao Qi, Xiaozhuo Zhang, Hui Zhang, Dongfang Shi, Qi Wang, Yu Li, Zhanwu Peng","doi":"10.1186/s12866-025-04036-x","DOIUrl":"10.1186/s12866-025-04036-x","url":null,"abstract":"<p><p>Myochromella mammosa, a novel species from southwestern China, is distinguished by its small, solitary or gregarious basidiomata. The pileus is clock-shaped, with distinct central mastoids, edge stripes, and a darker center that flattens upon maturation. The stipe is white, firm when young, and becomes hollow and slightly thickened at the base with age. We conducted a comprehensive analysis of publicly available ITS sequences from NCBI-GenBank and fungal amplicon sequencing data from NCBI-SRA to explore the global distribution and enumeration of recorded species of Myochromella. At a 98% sequence similarity threshold, eleven species were identified, including eight without formal designations. Myochromella's diversity is primarily Northern Hemisphere-centric and tends toward endemic distribution. Leveraging amplicon sequencing data sets enables precise species diversity assessments, enhancing field collection efficiency and providing novel insights into macrofungal species diversity.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"320"},"PeriodicalIF":4.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of aquatic clade 2 and 3 Campylobacter coli isolates from Slovenia reveals admixture with other Campylobacter species.","authors":"Stephan Lorenzen, Anastasia-Lisa Dieckmann, Sonja Smole Možina, Katja Zelenik, Pauline Marquardt, Aljoscha Tersteegen, Achim J Kaasch, Wolfgang Bohne, Andreas Erich Zautner","doi":"10.1186/s12866-025-04042-z","DOIUrl":"10.1186/s12866-025-04042-z","url":null,"abstract":"<p><p>Campylobacter coli, a significant foodborne pathogen, has undergone extensive genetic exchange with its close relative, Campylobacter jejuni, leading to the emergence of three distinct clades. While clade 1 strains are commonly isolated from clinical and agricultural sources, clades 2 and 3 are primarily found in aquatic environments. This study aimed to enhance our understanding of C. coli clade 2 and 3 isolates through genomic and phenotypic characterization. A total of 48 surface water samples were collected from 19 different water bodies throughout Slovenia, and eleven Campylobacter isolates initially identified as C. coli from clades 2 and 3 were cultured. Whole genome sequencing was then performed on these isolates. Phylogenetic analysis was conducted using core genome multilocus sequence typing (cgMLST) and k-mer analysis. Phenotypic characterization included growth analysis, autoagglutination, biofilm formation, motility, antimicrobial susceptibility, water survival, and metabolic profiling. Genomic analysis revealed significant admixture with other Campylobacter species in the clade 2 and 3 isolates. One isolate was found to represent a new species related to C. coli. Besides C. jejuni and C. lari, this novel species appears to have contributed to introgression in the C. coli clades 2 and 3 isolates. Phenotypic characterization demonstrated diverse growth patterns, motility, autoagglutination abilities, and biofilm formation among the isolates. This study provides new insights into the genetic diversity and phenotypic characteristics of aquatic C. coli clade 2 and 3 isolates from Slovenia. The observed admixture with other Campylobacter species highlights the complex evolutionary history of these environmental strains and underscores the importance of continued surveillance and characterization of Campylobacter isolates from diverse ecological niches.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"322"},"PeriodicalIF":4.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of Acinetobacter baumannii from environmental waters in Dhaka City, Bangladesh.","authors":"Mohammed Aziz Hossain, Nayara Noor E Fatima, Jahid H Tushar, Hasib Mahmud, Fahim Kabir Monjurul Haque","doi":"10.1186/s12866-025-04029-w","DOIUrl":"10.1186/s12866-025-04029-w","url":null,"abstract":"<p><p>Acinetobacter baumannii, a gram-negative bacterium commonly associated with nosocomial infections, has been relatively unexplored in the environmental context. The present study was conducted in Dhaka City, Bangladesh, with the primary objective of isolating and characterizing A. baumannii in environmental water sources. Surface water samples were collected from various water reservoirs to detect the presence of A. baumannii. Isolates were confirmed as A. baumannii using culture and PCR. Confirmed isolates were screened for antimicrobial susceptibility, antimicrobial resistance genes, and serum resistance. Results revealed that 32% of the water samples tested positive for A. baumannii. In total, 23 A. baumannii isolates were obtained. All isolates showed resistance to Cefepime. Varying degrees of resistance to other antibiotics were observed, and 56% showed resistance to the bactericidal effect of serum. This study underscored the remarkable adaptability of A. baumannii and its ability to flourish in diverse environmental conditions, highlighting public health concerns of increasing antibiotic resistant bacteria. The study concluded that, given the significance of effective infection control and sanitation and waste management measures, understanding the presence and behavior of A. baumannii in the environment is paramount. This study acts as the first report on environmental A. baumannii in Bangladesh and further research is warranted to elucidate the underlying mechanisms of antibiotic resistance and their implications for human health.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"314"},"PeriodicalIF":4.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of Extended Spectrum β-Lactamase Genes (ESBLs) among community and health care infection in Mansoura University Hospital, Egypt.","authors":"Alaa Aboelnour Badran, Fatma A Elgayar, Mona K Gouda, Nancy M El Halfawy","doi":"10.1186/s12866-025-04030-3","DOIUrl":"10.1186/s12866-025-04030-3","url":null,"abstract":"<p><strong>Background: </strong>Multidrug-resistant (MDR) Gram-negative bacteria pose a significant challenge due to their limited treatment options. The production of extended-spectrum β-lactamases (ESBLs) is an important mechanism of resistance. This study aimed to identify the incidence and characteristics of ESBL-encoding genes (bla_CTX-M, bla_TEM, bla_SHV, and bla_OXA) in MDR isolates.</p><p><strong>Materials and methods: </strong>A cross-sectional study was conducted from September 2022 to May 2023. ESBL-producing isolates (n = 105) out of 412 were recovered from hospitalized and outpatient settings and analyzed. Standard microbiological methods were used for isolates identification, susceptibility testing, and phenotypic ESBL detection. Additionally, bla_CTX-M, bla_TEM, bla_SHV, and bla_OXA genes were identified using conventional PCR.</p><p><strong>Results: </strong>Molecular profiling of β-lactamase determinants was conducted via PCR targeting bla_CTX-M, bla_TEM, bla_SHV, and bla_OXA genes. Among phenotypically confirmed (100%) ESBL producers, 98% harbored one or more target genes, with bla_CTX-M predominant (81%), followed by bla_SHV (70.4%), bla_TEM (62%), and bla_OXA (30.4%). Carbapenem resistance was higher in ESBL-producing strains compared to non-ESBL strains. Extensively drug-resistant (XDR) isolates were the most common across hospital departments and outpatients.</p><p><strong>Discussion: </strong>This study highlights the significant prevalence of ESBL genes and multidrug resistance among Gram-negative bacteria. The dominance of bla_CTX-M and the existence of multiple resistance genes raise concerns about limited treatment options. The findings emphasize the need for stricter antibiotic stewardship and infection control measures to curb the spread of MDR pathogens.</p><p><strong>Conclusion: </strong>This study provides valuable insights into the alarming incidence of ESBL genes and MDR in Mansoura, Egypt. Continuous surveillance and implementation of effective control strategies are crucial to combat this growing public health threat.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"316"},"PeriodicalIF":4.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic insights into microbial diversity and potential pathogenic transmission in poultry farm environments of Bangladesh.","authors":"Afroja Yasmin, M Shaminur Rahman, S M Kador, Md Mustak Ahmed, Md Eashanul Karim Moon, Humaira Akhter, Munawar Sultana, Anowara Begum","doi":"10.1186/s12866-025-03970-0","DOIUrl":"10.1186/s12866-025-03970-0","url":null,"abstract":"<p><p>The microbiome plays a critical role in poultry health and productivity, influencing growth, immunity, and the overall farm ecosystem. This study investigated microbial diversity, antibiotic resistance pathways, and functional potential across various components of poultry ecosystems-cloacal swabs, droppings, feed, hand swabs, soil, and water-in different districts of Bangladesh. Using 16S rRNA gene amplicon sequencing, we identified 2,745 Operational Taxonomic Units (OTUs) and analyzed microbial richness, community structure, and functional pathways. Alpha diversity metrics revealed that droppings exhibited the highest microbial richness (726 OTUs in Noakhali), while feed samples showed the lowest diversity (211 OTUs). Beta diversity analysis indicated significant differences in microbial composition across sample sources, with PERMANOVA confirming that sample origin accounted for 51.45% of the variability (p < 0.001). Proteobacteria dominated the microbial communities (48.36%), followed by Firmicutes (19.83%) and Cyanobacteria (12.02%). Key genera of concern, such as Enterobacter (26.62% in hand swabs), Acinetobacter (30.87% in cloacal swabs), and Shigella (22.89% in cloacal swabs), were identified, highlighting potential contamination and zoonotic risks. Conversely, beneficial genera like Lactobacillus (36.89% in feed) and Enterococcus (10.78% in droppings) were prevalent, suggesting roles in gut health and nutrient cycling. Functional pathway analysis (KEGG) revealed that carbohydrate and amino acid metabolism were highly active in droppings and feed, reflecting nutrient utilization. Antimicrobial resistance (AMR) pathways, such as 23S rRNA-methyltransferase and multidrug efflux pumps, were widespread, with pathogenic genera (Enterobacter, Acinetobacter, Shigella, Pseudomonas) showing strong positive correlations with AMR pathways. These findings underscore the influence of environmental factors on microbial diversity and functional potential in poultry farming. The study highlights the need for improved management practices and biosecurity measures to mitigate risks associated with microbial pathogens and antimicrobial resistance, ultimately supporting healthier and more sustainable poultry production in Bangladesh.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"318"},"PeriodicalIF":4.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary bacterial infections of Carbapenem-Resistant Acinetobacter baumannii in patients with COVID-19 admitted to Chinese ICUs.","authors":"Fuhong Chen, Jia Lin, Wei Yang, Jie Chen, Xiang Qian, Tao Yan, Xiuping Liu, Yewei Lu, Qi Chen","doi":"10.1186/s12866-025-04032-1","DOIUrl":"10.1186/s12866-025-04032-1","url":null,"abstract":"<p><strong>Background: </strong>A significant proportion of patients who are hospitalized with coronavirus disease 2019 (COVID-19), particularly those being admitted to ICUs, exhibit the development of secondary bacterial infections (SBIs). However, there is a lack of detailed epidemiological investigations and genetic information of Carbapenem-Resistant Acinetobacter baumannii (CRAB) based on whole genome sequencing (WGS), which is one of the frequently detected bacteria among COVID-19 patients, to confirm alterations in the clonal structure and infection mechanism.</p><p><strong>Results: </strong>A total of 37 unique CRAB strains, sourced from patients, along with an additional 2 CRAB strains form the environment, were isolated. Among the cohort of 37 patients, 22 individuals succumbed to CRAB infection, resulting in a mortality rate of 54.46%. The median duration of illness for these patients was 7.95 days, highlighting the severity and rapid progression of CRAB infections in this patient population. A total of 22 CRAB strains, isolated from deceased individuals, in addition to two strains isolated from the environment, were subjected to further investigation. All 24 CRAB isolates exhibited a high ability to form biofilm and displayed a similar spectrum of resistance. Except for two isolates from patients with COVID-19, all the remaining CRAB isolates were categorized as ST195 and demonstrated highly close genetic background based on analysis of WGS. The ST195 strain of CRAB harbored three copies of the bla_OXA-23 gene located on the chromosome, each of which was carried by Tn2006. Notably, one Tn2006 element was integrated within Tn6022, leading to the formation of AbaR4-like resistance islands Tn6166-I.</p><p><strong>Conclusions: </strong>Our findings underscore the significance of SBIs in the COVID-19 pandemic, particularly those caused by CRAB and specifically those belonging to MLST types that were previously prevalent in ICUs.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"319"},"PeriodicalIF":4.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of USP25 knockout on the gut microbial diversity and composition in mice.","authors":"Jinqiu Li, Zhonghui Chen, Xingchen Yan, Qizhou Chen, Cirong Chen, Huan Liu, Jianlin Shen","doi":"10.1186/s12866-025-04035-y","DOIUrl":"10.1186/s12866-025-04035-y","url":null,"abstract":"<p><strong>Background: </strong>The gut microbiota plays a crucial role in host health. Recent study revealed that ubiquitin-specific protease 25 (USP25) deficiency affected colonic immune responses and resistance to certain bacterial infection. This study aimed to investigate the impact of USP25 gene deletion on the gut microbiota of mice, utilizing 16 S rRNA amplicon sequencing and metagenomic sequencing to provide a comprehensive analysis of microbial diversity, composition and functional characteristics.</p><p><strong>Methods: </strong>We collected fecal samples from 10 wild type (WT) C57BL/6J mice and 10 USP25^-/- mice (C57BL/6J-Usp25^em1cyagen) for 16 S rRNA amplicon sequencing. Subsequently, the 6 of the 20 samples underwent further analysis using metagenomic sequencing.</p><p><strong>Results: </strong>Our results revealed significant differences in the gut microbiota between USP25 knockout (KO) mice and wild-type (WT) controls, with KO mice exhibiting 1,858 unique amplicon sequence variants (ASVs) compared to 1,723 in WT mice. Notably, the KO group displayed a higher tendency for biofilm formation and a greater proportion of gram-negative bacteria, while the WT group demonstrated enhanced stress tolerance and a higher presence of gram-positive bacteria. Functional prediction analyses indicated an increase in antibiotic resistance genes in the KO mice, particularly for tetracycline, cephalosporin, and sulfonamides, suggesting a potential risk for clinical antibiotic treatment efficacy. Moreover, KEGG pathway enrichment analysis revealed significant enrichment for fructose and mannose metabolism, streptomycin biosynthesis in the KO group. Furthermore, an increase in protective microbes alongside a decrease in potential pathogens in the KO microbiota hinted at altered immune responses due to USP25 deletion.</p><p><strong>Conclusion: </strong>Our findings elucidate the essential role of USP25 in modulating gut microbiota composition and function, providing insights for future therapeutic strategies targeting gut microbiota in disease contexts.</p><p><strong>Clinical trail number: </strong>Not applicable.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"315"},"PeriodicalIF":4.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of fermentation conditions for enhanced acetylcholine and biomass production of Lactiplantibacillus plantarum AM2 using the Taguchi approach.","authors":"Walid A Lotfy, Amira M Ali, Heba M Abdou, Khaled M Ghanem","doi":"10.1186/s12866-025-04017-0","DOIUrl":"10.1186/s12866-025-04017-0","url":null,"abstract":"<p><p>This study aimed to optimize the fermentation conditions and medium composition for maximum acetylcholine (ACh) and biomass production by Lactiplantibacillus plantarum AM2 using the Taguchi array design, which enables efficient identification of influential variables through minimal experimental runs. Seven key factors were evaluated: beef extract, peptone, yeast extract, glucose, pH, agitation rate, and inoculation size. The optimization process identified the most significant variables influencing ACh and biomass production, with beef extract and peptone being critical for ACh synthesis, while inoculation size was a critical determinant of biomass yield. The optimal conditions for ACh production were determined as beef extract (11 g/l), peptone (40 g/l), yeast extract (5 g/l), glucose (20 g/l), pH 5.7, no agitation, and 1% (v/v) inoculation size, resulting in a predicted ACh concentration of 490.83 pg/ml and an experimental value of 495.8 pg/ml. For biomass production, the optimal conditions were beef extract (8 g/l), peptone (10 g/l), yeast extract (20 g/l), glucose (35 g/l), pH 6.6, agitation at 150 rpm, and 4% (v/v) inoculation size, yielding a predicted biomass of 20.58 g/l and an experimental value of 21.3 g/l. The optimized conditions significantly improved ACh production (6.32-fold) and biomass production (4.56-fold) compared to basal conditions. These findings highlight the efficiency of the Taguchi approach in enhancing the production of ACh and biomass, providing insights into the functional niche of Lactiplantibacillus plantarum AM2 for potential industrial applications and its use in a symbiotic form.</p>","PeriodicalId":9233,"journal":{"name":"BMC Microbiology","volume":"25 1","pages":"317"},"PeriodicalIF":4.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}