Surveillance and characterization of carbapenem-resistant Enterobacter cloacae complex from China, 2015-2018.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

BMC Microbiology Pub Date : 2025-10-02 DOI:10.1186/s12866-025-04299-4

Kun Ye, Yongqing Zhang, Xuemei Qiu, Liyan Ye, Jiyong Yang, Yanning Ma

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引用次数: 0

Abstract

Background: The carbapenem-resistant Enterobacter cloacae (CREC) have become a global health concern. However, our understanding of its epidemiological trends and resistance mechanisms still needs to be improved.

Methods: Ninety-one non-repetitive clinical CREC isolates were collected from 12 hospitals across China and identified using MALDI-TOF MS, rMLST, ANI, MASH, and dDDH. Minimal inhibitory concentrations were determined via broth microdilution. Resistance genes and plasmid replicons were identified using ResFinder and PlasmidFinder. Carbonyl cyanide 3-chlorophenylhydrazone suppression test and OmpC and OmpF overexpression test were conducted on 32 non-carbapenemase-producing (NCP) isolates. A phylogenetic tree was constructed with kSNP4 and visualized through iTOL.

Results: Among the 91 CREC isolates, E. hormaechei was predominate (75 isolates), followed by E. kobei (five isolates). Within E. hormaechei, E. hormaechei subsp xiangfangensis was the most widespread subspecies. Sixty isolates produced carbapenemase, with bla_NDM-1 identified in 36 isolates, bla_KPC-2 in six isolates, and bla_NDM-5 in six isolates. Two isolates harbored both bla_NDM-1 and bla_KPC-2. Thirty-two isolates were NCP isolates, primarily due to carbonyl cyanide 3-chlorophenylhydrazone suppression.

Conclusions: Among CREC in China, the bla_NDM-1 was the dominant carbapenemase-coding gene. For NCP isolates, efflux pumps were the main reason for carbapenem resistance, and susceptibility could be restored in an antibiotic-free environment.

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2015-2018年中国耐碳青霉烯类阴沟肠杆菌的监测与特征分析

背景：耐碳青霉烯阴沟肠杆菌（CREC）已成为全球关注的健康问题。然而，我们对其流行趋势和耐药机制的认识仍有待提高。方法：从全国12家医院收集91株非重复性临床CREC分离株，采用MALDI-TOF MS、rMLST、ANI、MASH和dDDH进行鉴定。通过肉汤微量稀释测定最低抑菌浓度。利用ResFinder和PlasmidFinder对抗性基因和质粒复制子进行鉴定。对32株非产碳青霉烯酶（NCP）菌株进行了羰基氰化物3-氯苯腙抑制试验和OmpC、OmpF过表达试验。利用kSNP4构建系统发育树，并通过iTOL进行可视化。结果：91株CREC菌株中以荷氏大肠杆菌为主（75株），其次为北氏大肠杆菌（5株）。在霍马甲亚种中，霍马甲香方亚种分布最广。60株分离株产生碳青霉烯酶，其中blaNDM-1在36株分离株中鉴定，blaKPC-2在6株分离株中鉴定，blaNDM-5在6株分离株中鉴定。两个分离株同时携带blaNDM-1和blaKPC-2。32株为新型冠状病毒分离株，主要是由于羰基氰化物3-氯苯腙抑制所致。结论：在中国的CREC中，blaNDM-1是主要的碳青霉烯酶编码基因。对于新型冠状病毒分离株，外排泵是碳青霉烯类耐药的主要原因，在无抗生素环境下可恢复敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.