Biology and Fertility of Soils最新文献

{"title":"Pre-sowing recurrent inoculation with Pseudomonas fluorescens promotes maize growth","authors":"Marine Papin, Amélie Polrot, Marie-Christine Breuil, Sonia Czarnes, Assia Dreux-Zigha, Xavier Le Roux, Ahmed Taibi, Aymé Spor, Laurent Philippot","doi":"10.1007/s00374-024-01873-2","DOIUrl":"https://doi.org/10.1007/s00374-024-01873-2","url":null,"abstract":"<p>Despite the rapid development of microbial inoculants use, their effectiveness still lacks robustness, partly due to our limited understanding of the factors influencing their establishment in soil. Recurrent inoculation can temporarily increase their abundance, but the effect of this inoculation strategy on plant growth and on the resident microbial community is still poorly studied. Here, we investigated maize growth and soil bacterial community responses under recurrent inoculation of the plant-beneficial bacterium <i>Pseudomonas fluorescens</i> B177. We further assessed how the effect of recurrent inoculation was modulated by the inoculant dose, the application timing and the soil type. Recurrent inoculation at high dose transiently increased the abundance of <i>P. fluorescens</i> B177 and resulted in larger shifts in the resident bacterial community compared to a single inoculation event. Moreover, recurrent inoculation prior to sowing had the strongest effect on maize growth, with increased shoot dry weight by 47.4%, likely due to an indirect effect of the inoculant through early changes in the resident community. Altogether these findings highlight the significance of recurrent pre-sowing inoculations as an alternative strategy for promoting plant growth.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"14 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactive effects of plant litter chemistry and organic/inorganic forms of nitrogen addition on Moso bamboo (Phyllostachys edulis) soil respiration","authors":"Shoujia Zhuo, Yunying Fang, Youchao Chen, Tony Vancov, Huaqiang Du, Yongfu Li, Bing Yu, Scott X. Chang, Yanjiang Cai","doi":"10.1007/s00374-024-01875-0","DOIUrl":"https://doi.org/10.1007/s00374-024-01875-0","url":null,"abstract":"<p>The impact of plant litter on soil carbon (C) cycling is influenced by external nitrogen (N) deposition and plant litter chemistry. While previous research has mainly focused on inorganic N deposition and its effect on plant litter decomposition and soil C cycling, the influence of organic N remains poorly understood. In this study, we conducted a 180-day incubation experiment to investigate how different N forms (NH₄NO₃, Urea 50% + Glycine 50%) and litter chemistry (varying lignin/N ratios) affect CO₂ emissions from an acidic Moso bamboo (<i>Phyllostachys edulis</i>) forest soil. Our findings indicate that litter addition increased soil CO₂ emissions and the proportion of CO₂-C to Total C (considering added litter-C as a part of total C). Specifically, Moso bamboo leaf litter with a lower lignin/N ratio led to higher soil CO₂ emissions and CO₂-C/Total C ratios. The combined addition of litter and N exhibited an antagonistic effect on soil CO₂ emissions, with inorganic N having a more pronounced effect compared to organic N. This antagonistic effect was attributed to the N addition-induced soil acidification, thereby inhibiting microbial activities and reducing soil respiration promoted by litter input. This effect was confirmed by random forest analysis and partial least squares path modeling, which further identified soil dissolved organic C and pH as critical factors positively influencing soil CO₂ emissions. Overall, our study suggests that atmospheric N deposition can mitigate litter-induced soil CO₂ emissions, particularly under inorganic N forms and when leaf litters with high lignin/N ratios are introduced.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"14 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the influence of intercropping and arbuscular mycorrhizal inoculation on two modern durum wheat cultivars and their associated microbiota","authors":"Elisa Zampieri, Fabiano Sillo, Giulio Metelli, Maria Alexandra Cucu, Vincenzo Montesano, Giulia Quagliata, Lena Philipp, Francesca Brescia, Adriano Conte, Luca Giovannini, Carmelo Mennone, Angelo Fiore, Stefania Astolfi, Daniel Savatin, Francesco Sestili, Thomas Reitz, Raffaella Balestrini","doi":"10.1007/s00374-024-01872-3","DOIUrl":"https://doi.org/10.1007/s00374-024-01872-3","url":null,"abstract":"<p>Intercropping, based on the interplay between cereals and legumes, might be an encouraging approach to improve soil fertility and crop productivity and to guarantee more sustainable farming systems. However, plant consociation is also influenced by the interaction between roots and soil microbial communities, and different plant genotypes might differently respond to this management. Here, a 2-year field study was carried out, verifying the impact of intercropping and the inoculation with arbuscular mycorrhizal fungi (AMF) on two varieties of durum wheat, using a lentil variety as intercropped plant species, on wheat agronomic parameters and grain features, as well as on microbial communities of soil, rhizosphere and wheat roots. Results showed a genotype effect on diverse agronomic parameters, gluten quality and grain elemental concentrations. Additionally, intercropping and AM fungal inoculation affected and shaped the microbial alpha diversity and composition, especially for the AMF community, at root level. Overall, the effects of the considered treatments (intercropping with lentil and AM fungal inoculation) were noticeably influenced by the specific wheat genotype, suggesting the importance to conduct a careful selection of intercropped genotypes.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"65 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Land use drives prokaryotic community composition of directly adjacent grasslands","authors":"Rubén Martínez-Cuesta, Anna Holmer, Franz Buegger, Michael Dannenmann, Michael Schloter, Stefanie Schulz","doi":"10.1007/s00374-024-01871-4","DOIUrl":"https://doi.org/10.1007/s00374-024-01871-4","url":null,"abstract":"<p>Understanding the impact of agricultural land use on the soil prokaryotic communities in connected downslope sites is crucial for developing sustainable strategies to preserve ecosystem properties and mitigate agriculture’s environmental impacts. In this study, we investigated topsoil samples collected at three time points in 2022 (March, June, and November) from two adjacent catenas, reaching from hillslope to floodplain. The catenas differed in land use (extensive grassland vs. extensive cropland) at the top and middle parts, while the floodplain remained an extensive grassland due to legal restrictions. Using quantitative real-time PCRs and metabarcoding, we assessed prokaryotic abundance and prokaryotic community composition. Results show higher bacterial abundance in the cropland-influenced floodplain part across all time points compared to the grassland-influenced floodplain part. Temporal dynamics revealed a progressive decrease in the shared prokaryotic communities of the floodplain parts, peaking at the summer sampling time point, indicating a significant influence of the respective management type of the agricultural sites over the bacterial and archaeal communities of the floodplain parts. Differential abundance analyses identified several nitrifying taxa as more abundant in the cropland-influenced floodplain. Upstream land use also influenced the prokaryotic network of the cropland-floodplain, with some cropland taxa becoming keystone taxa and altering network morphology, an effect not observed in the grassland-influenced floodplain. These findings suggest that upstream agricultural land use practices have exerted a long-term influence on the floodplain prokaryotic communities over the past three decades. Moreover, there is evidence suggesting that these prokaryotic communities may undergo a potential reset during winter, which requires further investigation.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"31 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metatranscriptomic analysis to reveal the coupling between nitrogen fixation and CH4 oxidation in root tissues of Phragmites australis","authors":"Zhihua Bao, Jing Cui, Jumei Liu, Meng Zhang, Linxia Chen, Weiwei Cao, Ke Yu, Lixin Wang, Zhongjun Jia, Ji Zhao","doi":"10.1007/s00374-024-01869-y","DOIUrl":"https://doi.org/10.1007/s00374-024-01869-y","url":null,"abstract":"<p>The root-associated type II methanotrophs significantly contribute to CH₄ oxidation-dependent N₂ fixation. However, it is unclear whether type I methanotrophs are involved in CH₄ oxidation and N₂ fixation, especially in natural wetlands. So far, limited attention has given to root-associated active microorganisms. Here, metatranscriptomic analysis of root-associated microbes has been proposed to reveal the aerobic methanotrophs contributing to CH₄ and nitrogen cycles in the roots of <i>Phragmites australis</i> grown in a natural wetland. Results showed Methylocystaceae (type II methanotrophs) and Methylococcaceae (type I methanotrophs) as major taxa (relative abundance, 14%) at transcription level. However, based on 16S rRNA gene sequencing, contribution of these taxa was &lt; 1% at DNA level. Genes encoding methane monooxygenase (enzyme responsible for the first step of CH₄ oxidation) were detected in <i>Methylomonas</i> (<i>pmoCBA</i>) and <i>Methylosinus</i> (<i>mmoXYZCB</i>). Furthermore, genes related to methanol dehydrogenase, formaldehyde dehydrogenase, and formate dehydrogenase were also detected in <i>Methyosinus</i> and <i>Methylomonas</i>, while <i>mcrA</i> gene was observed in <i>Methanospirillum</i> and <i>Methanofollis</i>. Moreover, nitrogenase structural genes, such as <i>nifHDK,</i> were found in <i>Methylosinus</i> (Methylocystaceae) and <i>Methylomonas</i> (Methylococcaceae). Minor nitrogenase genes were detected in <i>Cyanothece</i>, <i>Lyngbya</i>, <i>Pelobacter</i> and <i>Smithella</i> of Cyanobacteriaceae family. In addition, N₂ fixing activity of <i>P. australis</i> was determined by analyzing the natural abundance of δ¹⁵N from June to August. The N₂ fixing activity of <i>P. australis</i> increased in presence of CH₄ in root system under ¹⁵N-N₂ feeding. Metatranscriptomic analysis revealed that not only type II methanotrophs, but also type I methanotrophs oxidize CH₄ and fix N₂.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"11 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the survival rate and effectiveness of plant growth-promoting bacteria through bioencapsulation techniques","authors":"Luz de-Bashan, Juan D. Giraldo, Mauricio Cruz-Barrera, Mauricio Schoebitz","doi":"10.1007/s00374-024-01870-5","DOIUrl":"https://doi.org/10.1007/s00374-024-01870-5","url":null,"abstract":"<p>In the absence of an appropriate formulation, the population of plant growth-promoting bacteria (PGPB) inoculated into soil may be significantly reduced. These unprotected introduced bacteria must compete with the often-more adapted native microflora and are susceptible to predation by soil microfauna. This opinion paper addresses the significance of proper formulation in creating an effective inoculant, discusses the primary challenges associated with current liquid and dry formulations, and emphasizes the rationale for bioencapsulation as the optimal approach for protecting PGPB in a successful inoculant.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"13 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142385496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced CO2 emissions from soil organic matter in agricultural fields during microbial community assemblage","authors":"Guozhen Gao, Haiyan Cui, Pengfa Li, Shiyu Ma, Ming Liu, Meng Wu, Zhongpei Li","doi":"10.1007/s00374-024-01868-z","DOIUrl":"https://doi.org/10.1007/s00374-024-01868-z","url":null,"abstract":"<p>Using two kinds of microbial inoculations extracted from soil cropped to rice and peanut, we conducted a swap-inoculation experiment to explore the relative importance of microbial inoculation and soil properties on CO₂ emissions from soil. Inoculated microorganisms into a soil different from their origin (swap inoculation) were partially successful and reduced CO₂ emissions, namely according to home-field advantage (HFA); The success of invasive microorganisms depended on molecular composition of soil organic matter (SOM) compared to inoculation of native microbes (inoculated microorganisms into origin soil). The different habits screened the fewer microorganisms to undergo respiration for energy and life-sustaining activities, thus decreasing CO₂ emissions from SOM. However, the effect of HFA diminished with incubation time, as the invasive microorganisms reshaped SOM molecular diversity and composition during microbial community assemblage, which fits with the Gaia effect (GE). Specific microbial communities, such as <i>Bacteroidetes</i> and <i>Actinobacteria</i>, drove the conversion of persistent molecules to labile molecules, thereby increasing the chances of SOM mineralization by microorganisms. We found there was positive correlation between labile SOM molecules and SOM mineralization. In addition, MBC increased in swap inoculation compared to native inoculation after 60 days, which also resulted in higher CO₂ emissions from SOM. HFA and GE provide new perspectives to help decipher the interaction between microorganisms and the habitat under microbial invasion, and the mechanism of influence on CO₂ emissions from SOM.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"32 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142385495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated rather than organic farming history facilitates soil nitrogen turnover and N2O reduction in a green rye – silage maize cropping sequence","authors":"Fawad Khan, Samuel Franco-Luesma, Michael Ulrich Dannenmann, Rainer Gasche, Andreas Gattinger, Frederik Hartmann, Beatrice Tobisch, Ralf Kiese, Benjamin Wolf","doi":"10.1007/s00374-024-01865-2","DOIUrl":"https://doi.org/10.1007/s00374-024-01865-2","url":null,"abstract":"<p>Soil gross mineral N production and consumption processes are crucial regulators of plant productivity and N loss from croplands. Substituting synthetic fertilizers by integrating legumes in cultivation systems is common in organic farming, but research on its long-term impact on dynamics of gross soil N transformation and associated environmental N loss is scarce. In particular, studies at a temporal resolution that allows for a mechanistic understanding of long-term effects of organic farming are missing. Therefore, we determined gross N turnover rates of ammonification, nitrification, and ammonium and nitrate immobilization at monthly temporal resolution during a full green rye-maize cropping sequence. Measurements were carried out at sites with same pedo-climatic background but organic farming (OF) and integrated farming (IF) history. During green rye growing, N turnover rates for OF and IF were low and not significantly different, likely owing to low temperatures. During silage maize growing, IF exhibited significantly higher average N turnover rates of 1.86, 4.46, and 5.57 mg N kg⁻¹ dry soil d⁻¹ for gross ammonification, ammonium immobilization, and nitrate immobilization, respectively, compared to OF values of 1.11, 1.80, and 2.90 mg N kg⁻¹ dry soil d⁻¹. The significantly higher N turnover rates were likely due to higher soil organic C, N and microbial biomass which result from different long-term management practices. Especially the increased immobilization potential on the IF site contributed to significantly lower area-scaled N₂O emissions (1.45 vs. 4.36 kg N ha⁻¹) during periods of high nitrification. This shows that for low SOC soils, integrated farming history with high C return enhances soil N cycling and reduces the risk of N losses in the form of N₂O emission.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"31 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of acetochlor degradation and bacterial community in black soil to the application of vermicompost","authors":"Xia Hou, Xinhong Wang, Yang Ou, Liming Yan, Huiping Liu, Xinyi Li, Minglian Shang","doi":"10.1007/s00374-024-01867-0","DOIUrl":"https://doi.org/10.1007/s00374-024-01867-0","url":null,"abstract":"<p>Acetochlor (ACE), one of the widely used herbicides in northeastern China, has raised concerns due to its residual presence in the soil. In this study, a pot experiment was conducted to investigate the effects of adding vermicompost on the degradation efficiency and pathways of acetochlor in black soil under dark conditions. The results showed that the vermicompost addition increased the degradation rate of acetochlor, shortened its degradation half-life, and altered the composition of the bacterial community. The influence of vermicompost on bacterial community diversity is minimal, but it can increase the relative abundance of acetochlor degradation bacteria, promoting the collaboration between exogenous and indigenous bacteria to enhance acetochlor utilization. GC-MS analysis revealed the formation of seven metabolites during the acetochlor degradation process, including 2-chloro-N-(2-ethyl-6-methylphenyl) acetamide, 2-ethyl-6-methylaniline, 4-amino-3-ethyl-5-methylpheno, 2-ethyl-6-methylcychexa-2,5-diene-1,4-diol, 2-ethyl-6-methylcychexa-2,5-diene-1,4-dione, N-(2-ethyl-6-methylphenyl)hydroxylamine and 1-ethyl-3-methyl-2-nitrobenzene. The synergistic action of <i>Sphingomonas</i>, <i>Rhodococcus</i>, <i>Bacillus</i>, <i>Arthrobacter</i>,<i> Methylobacillus</i>, and <i>Streptomyces</i> probably lead to the gradual decomposition of acetochlor into H₂O and CO₂. Comparative analysis of functional genes in the KEGG metabolic pathways showed upregulation of hyaB/hybC, hyaA/hybO, nfsA, nfnB/nfsB, and nemA in the soil treated with vermicompost. These functional genes could promote -NHOH conversion to -NO₂. Additionally, redundancy analysis revealed that soil organic matter and pH were the main driving factors for bacterial community variation. These findings suggest that vermicompost can be used as a bioremediation measure to reduce acetochlor in black soil.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"55 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen input differentially shapes the rhizosphere microbiome diversity and composition across diverse maize lines","authors":"Hussnain Mukhtar, Jingjie Hao, Gen Xu, Emma Bergmeyer, Musa Ulutas, Jinliang Yang, Daniel P. Schachtman","doi":"10.1007/s00374-024-01863-4","DOIUrl":"https://doi.org/10.1007/s00374-024-01863-4","url":null,"abstract":"<p>Despite the crucial role of microbial communities in agroecosystem functioning, a clear picture of how nitrogen shapes rhizosphere microbial complexity and community structure across diverse maize lines remains elusive. To address this gap, we conducted 16S amplicon sequencing of the rhizosphere microbial communities across a diverse range of maize inbred lines (305 genotypes) and their F1 hybrids (196 genotypes) cultivated in both low-nitrogen (unfertilized) and high-nitrogen (fertilized) soils. Our findings reveal that N fertilizer treatment had contrasting effects on the rhizosphere microbial communities of inbreds and hybrids. N fertilization increased alpha diversity but decreased the abundance of <i>Pseudomonas</i> taxa in inbred lines, while the opposite was true for hybrids. The proportion of variance determined by plant host factors was also better explained under low-N, demonstrating that N fertilization reduced the influence of the host over the rhizosphere microbial community. Microbial networks revealed significant differences in the number of nodes and clustering coefficients between the rhizosphere microbial communities of inbred and hybrid maize, with these differences being further differentiated by changes in nitrogen levels. Overall, our study reveals the interplay among rhizosphere microbiomes, abiotic stress induced by low soil nitrogen, and plant host factors facilitating the identification of stable microbial communities in response to environmental stress. These findings contribute to the potential engineering of resilient microbial consortia highlighting the importance of the influence of plant genotype and the environment on the rhizosphere microbiome.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"30 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142321533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}