Biology and Fertility of Soils最新文献

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Does an enhanced microbial diversity promote the resistance of soil multifunctionality against drought events in amended soils?
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-04-10 DOI: 10.1007/s00374-025-01914-4
L. Morales-Salmerón, E. Fernández-Boy, B. Herrador, R. León, M. T. Domínguez
{"title":"Does an enhanced microbial diversity promote the resistance of soil multifunctionality against drought events in amended soils?","authors":"L. Morales-Salmerón, E. Fernández-Boy, B. Herrador, R. León, M. T. Domínguez","doi":"10.1007/s00374-025-01914-4","DOIUrl":"https://doi.org/10.1007/s00374-025-01914-4","url":null,"abstract":"<p>A large fraction of the Mediterranean soils is threatened by losses of organic matter and biodiversity, which could compromise the provision of soil ecosystem services and the stability of ecosystems in the face of climate change. In this work we explore several hypotheses related to the role of C inputs and microbial diversity on soil multifunctionality and its resistance to drought in degraded Mediterranean soils. We designed a factorial experiment to test the effect of the addition of an organic amendment and of microbial diversity (using four inoculants with different abundance and diversity of soil microbiota), on the resistance of soil functionality against drought in pot mesocosms. Pots were sown with a forage mixture (<i>Lolium rigidum</i> and <i>Medicago polymorpha</i>), and plant productivity, soil chemical properties, and microbial activity and diversity were measured before and after a simulated drought event. The amendment favored soil moisture, enhancing the stability of the productivity of <i>M. polymorpha</i>. In contrast, the manipulation of inoculation load had a limited effect on the resistance of microbiological activity. Indeed, microbial functioning was highly resistant to reduced water inputs, probably related to the prevalence of Gram positive bacteria. Besides, the effect of microbial diversity on soil multifunctionality was limited. Structural equation modelling confirmed that the enhancement of multifunctionality after soil amendment was attributed to the direct effect of organic C on soil moisture and chemical fertility. In these degraded soils, physico-chemical limitations are the major drivers of soil multifunctionality rather than bacterial or fungal diversity.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"183 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phosphorus fertilizer enhances the tolerance of rhizosphere microbial community to low-light stress in Tartary buckwheat
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-04-08 DOI: 10.1007/s00374-025-01915-3
Yuchuan Zhang, Zhijia Cui, Yujiao Li, Meng Wang, Feifei Zhang, Yu Feng, Xi Zhang, Qinghua Yang, Lixin Tian, Baili Feng
{"title":"Phosphorus fertilizer enhances the tolerance of rhizosphere microbial community to low-light stress in Tartary buckwheat","authors":"Yuchuan Zhang, Zhijia Cui, Yujiao Li, Meng Wang, Feifei Zhang, Yu Feng, Xi Zhang, Qinghua Yang, Lixin Tian, Baili Feng","doi":"10.1007/s00374-025-01915-3","DOIUrl":"https://doi.org/10.1007/s00374-025-01915-3","url":null,"abstract":"<p>Low-light stress has become an important factor limiting crop yield and quality improvement. Appropriate phosphorus (P) addition can enhance soil microbial activity and nutrient availability, thereby alleviating the negative impacts of low-light stress. However, the role of crop rhizosphere microorganisms in the mitigation of low-light stress by P addition in agroecosystems remains unclear. In this study, three light conditions (normal light, S0; moderate low-light stress, S1; severe low-light stress, S2) and three P addition levels (0 kg ha<sup>− 1</sup>, P0; 35 kg ha<sup>− 1</sup>, P1; 70 kg ha<sup>− 1</sup>, P2) were applied to analyze the combined effects on the rhizosphere microbial diversities, compositions, co-occurrence patterns, and assembly mechanisms during critical growth stages (flowering, filling, and maturity stages). The results showed that both low-light stress and P appreciably affected rhizosphere microbial community composition, with P promoting the proliferation of rhizosphere beneficial microbes under low-light stress. Low-light stress reduced rhizosphere microbial α-diversity, and S2 simplified microbial networks. In contrast, P1 increased bacterial network complexity, connectivity, and stability. Indicator taxa analysis revealed that P1 increased the abundance of shared and specific species in rhizosphere microbial networks. Under S1, P1 enhanced keystone taxa abundance. Community assembly analysis indicated that bacterial communities were governed by deterministic processes, whereas low-light stress reduced fungal stochasticity, which was increased by P addition. These findings highlight that P addition under low-light stress can enhance the tolerance of Tartary buckwheat rhizosphere microbial community by modulating rhizosphere microbial diversity, composition, and network stability, providing insights into alleviating low-light stress.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"24 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exudate pulses throughout the entire growth period trigger the increase in maize phosphorus use efficiency by modifying soil keystone microbial taxa
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-04-04 DOI: 10.1007/s00374-025-01912-6
Guiwei Wang, Yuechao Yang, Yuanyuan Yao, Xiaoqi Wang
{"title":"Exudate pulses throughout the entire growth period trigger the increase in maize phosphorus use efficiency by modifying soil keystone microbial taxa","authors":"Guiwei Wang, Yuechao Yang, Yuanyuan Yao, Xiaoqi Wang","doi":"10.1007/s00374-025-01912-6","DOIUrl":"https://doi.org/10.1007/s00374-025-01912-6","url":null,"abstract":"<p>Exudates are the medium through which plants adapt to complex soil environments, however, the mechanisms of how different types of root exudates increase maize yield and phosphorus (P) use efficiency (PUE) throughout the entire growth period remains unknown. In this study, we designed an experiment to examine the effects of continuous addition of exudate substances on maize growth and P uptake over the entire growth period. The addition of succinic acid, luteolin, and inositol significantly increased maize biomass, particularly root biomass. Specifically, the treatment with added succinic acid increased maize yield by 11.6% and PUE by 8%. Additionally, we found that different exudate substances significantly altered the soil bacterial and fungal communities, thereby increasing soil P bioavailability. The microbial co-occurrence networks revealed that Actinobacteriota and Proteobacteria keystone ASVs, enriched by the addition of succinic acid, luteolin, and inositol, were significantly associated with maize P uptake. Furthermore, at the V12 stage (Twelve leaves unfolded), the addition of exudate substances significantly increased alkaline phosphatase activity, and at the R6 stage (Maturity), soil available P content was significantly elevated, enhancing soil P bioavailability. These findings provide evidence for future exploration of the interaction mechanisms between plants and soil microbes and optimizing nutrient management strategies in farmland.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"183 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soil bacterial and fungal communities beneath different forest types differentially and promptly respond to non-catastrophic typhoon disturbance
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-03-31 DOI: 10.1007/s00374-025-01913-5
Zhihui Wang, Rui Zhang, Rui Cao, Lifeng Wang, Zhuang Wang, Qin Wang, Yuchen Lu, Yong Zhang, Wanqin Yang
{"title":"Soil bacterial and fungal communities beneath different forest types differentially and promptly respond to non-catastrophic typhoon disturbance","authors":"Zhihui Wang, Rui Zhang, Rui Cao, Lifeng Wang, Zhuang Wang, Qin Wang, Yuchen Lu, Yong Zhang, Wanqin Yang","doi":"10.1007/s00374-025-01913-5","DOIUrl":"https://doi.org/10.1007/s00374-025-01913-5","url":null,"abstract":"<p>The immediate responses of soil microbial communities to non-catastrophic typhoon disturbances remain largely unclear, despite soil microorganisms are key contributors to ecosystem functioning and sensitive indicators to forest disturbances. To address this gap, we simultaneously investigated soil microbial communities beneath bamboo forest (BF), Chinese fir forest (CF), secondary broadleaf forest (SF) and mixed forest (MF) in the subtropical mountain area of southeastern China before and after typhoon disturbances. Typhoon disturbances significantly altered the composition of soil microbial communities, decreasing bacterial diversity by 12.3% and network robustness by 22.6% in the topsoil, while increasing fungal diversity by 75.0% and network robustness by 33.2% in the litter layer. Notably, these indices returned to the per-typhoon levels post-disturbance. Meanwhile, typhoon disturbances increased the abundance of functional guild on bacterial cellular processes, fungal dung saprotrophs and plant saprotrophs in the topsoil. Moreover, the immediate responses of microbial community composition and diversity to typhoon disturbances were more pronounced in the CF and SF, and bacterial communities were more responsive in the topsoil, while fungal communities were more responsive in the litter layer. Additionally, soil temperature was the primary driver of microbial communities in the topsoil, while litter cellulose and calcium concentrations were key factors in the litter layer. Besides, carbon and nutrient concentrations were also significantly correlated with the composition of taxa and functional guilds of soil microbial communities. In summary, the immediate responses of microbial communities to typhoon disturbances vary considerably depending on forest types and soil horizon, with rapid recovery following typhoon events.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"72 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of methods for assessing fungi-to-bacteria ratio of soil
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-03-27 DOI: 10.1007/s00374-025-01911-7
Miikka B. Laine, Sami J. Taipale, Marja Tiirola
{"title":"Comparison of methods for assessing fungi-to-bacteria ratio of soil","authors":"Miikka B. Laine, Sami J. Taipale, Marja Tiirola","doi":"10.1007/s00374-025-01911-7","DOIUrl":"https://doi.org/10.1007/s00374-025-01911-7","url":null,"abstract":"<p>Measuring bacterial and fungal biomass may offer insights into agroecosystem health. Nevertheless, few studies have directly compared the ability of different methods to assess the abundance of these two microbial groups and their ratio (F/B ratio). This study compared the ability, precision, and repeatability of three commonly used laboratory methods - phospholipid fatty acid (PLFA) analysis, quantitative PCR (qPCR), and droplet-digital PCR (ddPCR) - alongside a commercially available microbial carbon testing tool (microBIOMETER<sup>®</sup>), to assess the F/B ratio and microbial abundance in agroecosystem soils. We also reviewed recent literature on common measurement and reporting practices. PLFA and ddPCR provided the most reliable outcomes, with PLFA being the most precise, repeatable, and widely used (81% of reviewed studies). However, significant variability in analytical procedures exists between laboratories, and key details, such as storage conditions, are often underreported. MicroBIOMETER<sup>®</sup> can offer a low-cost option for assessing total microbial biomass, but did not match PLFA results in determining the F/B ratio. ddPCR offered better precision than qPCR but had a narrower dynamic range. Therefore, optimal approach is to use the two methods in parallel. In conclusion, we recommend future studies adopt PLFA analysis as the primary method for assessing microbial abundance and F/B ratio of soils, as PCR-based measurements are influenced by several unavoidable biases. Furthermore, we suggest improvements to the PLFA method to ensure more reliable comparisons across laboratories. Altogether, our study gives guidelines for improving the monitoring of F/B ratio and microbial abundance in agroecosystems.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"18 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inoculation with in vitro promising plant growth-promoting bacteria isolated from nitrogen-limited boreal forest did not translate to in vivo growth promotion of agricultural plants
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-03-25 DOI: 10.1007/s00374-025-01910-8
Tinkara Bizjak-Johansson, Anne Braunroth, Regina Gratz, Annika Nordin
{"title":"Inoculation with in vitro promising plant growth-promoting bacteria isolated from nitrogen-limited boreal forest did not translate to in vivo growth promotion of agricultural plants","authors":"Tinkara Bizjak-Johansson, Anne Braunroth, Regina Gratz, Annika Nordin","doi":"10.1007/s00374-025-01910-8","DOIUrl":"https://doi.org/10.1007/s00374-025-01910-8","url":null,"abstract":"<p>Many yet undiscovered plant growth-promoting bacteria are proposed to be harboured in the nitrogen-limited boreal forest. These bacteria are suggested to increase plant growth not only due to their ability to fix nitrogen but also through other growth-promoting properties. Therefore, this study looked at the plant growth promotion potential of endophytic bacteria isolated from boreal forest conifer Scots pine (<i>Pinus sylvestris</i>) needles. Seven assays were used to measure the potential plant growth-promoting abilities of two newly isolated bacteria in this study and seven additionally selected bacteria isolated in our previous study. The three best-performing bacteria were used, either individually or in a consortium, to assess growth promotion on four common crop species. The greenhouse study included the presence of native soil and seed microbiota and used naturally nutrient-abundant soil. The results showed that while all bacteria were capable of multiple plant growth-promoting properties in the in vitro assays, they did not promote plant growth in the in vivo experiment as inoculated plants had similar or decreased chlorophyll content, root and shoot length and dry biomass compared to control plants. Our results show that bacterial plant growth-promoting potential does not always translate into successful plant growth increase in in vivo conditions and highlight the need for a better understanding of plant-bacteria interaction for the future establishment of successful bacterial bioinoculants.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"41 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143695671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nitrous oxide fluxes, their sources, and soil microbial communities depend more on carbon availability than long- and short-term phosphorus addition
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-03-24 DOI: 10.1007/s00374-025-01909-1
Pauline Sophie Rummel, Anthony Imoudu Oyeogbe, Haitao Wang, Tim Urich, Nicole Wrage-Mönnig
{"title":"Nitrous oxide fluxes, their sources, and soil microbial communities depend more on carbon availability than long- and short-term phosphorus addition","authors":"Pauline Sophie Rummel, Anthony Imoudu Oyeogbe, Haitao Wang, Tim Urich, Nicole Wrage-Mönnig","doi":"10.1007/s00374-025-01909-1","DOIUrl":"https://doi.org/10.1007/s00374-025-01909-1","url":null,"abstract":"<p>Denitrification is the key process leading to production and loss of nitrogen gases from soils. Its main drivers are N availability and soil water content, but interactions with other elements, such as carbon and phosphorus, can also influence N<sub>2</sub>O formation. So far, robust information on the effects of P and the historical context of P addition on N<sub>2</sub>O sources remains limited. To address this knowledge gap, we conducted a mesocosm chamber experiment using isotopic approaches to investigate N transformations and N<sub>2</sub>O sources following P fertilizer addition in soils with varying histories of P fertilization (low and high P). Differences in long-term fertilization affected C, N, and P availability as well as microbial community composition and nutrient cycling processes. Initially, microbes in both soils were C-limited with slightly higher C availability and microbial respiration in high P soils. Low P availability in low P soil did not restrict denitrification. In contrast, long-term P-unfertilized soil had higher N<sub>2</sub>O losses compared to high P soil, which were further stimulated with P addition. Glucose addition alleviated C limitation and strongly promoted microbial growth and respiration, but did not affect N<sub>2</sub>O emissions among treatments. Bacterial denitrification and nitrifier denitrification were the main N<sub>2</sub>O forming processes, while dissimilatory nitrate reduction to ammonium (DNRA) contributed to NO<sub>3</sub><sup>−</sup> reduction, but only slightly to N<sub>2</sub>O formation.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"20 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increasing phosphorus availability reduces priming effect by facilitating microbial carbon use efficiency in a subtropical forest soil
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-03-21 DOI: 10.1007/s00374-025-01906-4
Quanxin Zeng, Qiufang Zhang, Kongcan Mei, Jiguang Feng, Xiaochun Yuan, Yuanyuan Liu, Min Xu, Hao Sun, Biao Zhu, Yuehmin Chen
{"title":"Increasing phosphorus availability reduces priming effect by facilitating microbial carbon use efficiency in a subtropical forest soil","authors":"Quanxin Zeng, Qiufang Zhang, Kongcan Mei, Jiguang Feng, Xiaochun Yuan, Yuanyuan Liu, Min Xu, Hao Sun, Biao Zhu, Yuehmin Chen","doi":"10.1007/s00374-025-01906-4","DOIUrl":"https://doi.org/10.1007/s00374-025-01906-4","url":null,"abstract":"<p>The mechanisms by which phosphorus (P) availability regulates the priming effect (PE) induced by the addition of leaf litter with different qualities remain unclear. Here, soil samples from a subtropical <i>Pinus massoniana</i> forest were added with/without P and/or high- and low-quality <sup>13</sup>C-labeled leaf litter. The samples were then incubated in the laboratory for 75 days to assess the PE, microbial community composition, enzyme activity, and microbial carbon use efficiency (CUE). The results showed that litter addition led to a positive PE. High-quality litter inputs stimulated microbial activity but reduced microbial CUE, resulting in a higher PE intensity. By contrast, the PE exhibited a decrease with P addition. Such finding indicates that strategies for obtaining P, such as microbial decomposition of soil organic matter, may be reduced. The random forest analysis revealed that microbial CUE is the dominant factor regulating PE, accounting for 62% of the variation in PE, and it exhibited a negative effect on PE. Collectively, our findings emphasize that P availability regulates PE by decreasing microbial decomposition and increasing CUE, highlighting its essential role in carbon-climate feedbacks.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"26 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional shift in soil microbiome with decline in photosynthetic carbon input signifies rapid decrease of soil organic carbon during alpine grassland degradation
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-03-19 DOI: 10.1007/s00374-025-01907-3
Minghua Song, Rui Pang, Yuqiang Tian, Yikang Li, Guiqiang Wang, Xingliang Xu
{"title":"Functional shift in soil microbiome with decline in photosynthetic carbon input signifies rapid decrease of soil organic carbon during alpine grassland degradation","authors":"Minghua Song, Rui Pang, Yuqiang Tian, Yikang Li, Guiqiang Wang, Xingliang Xu","doi":"10.1007/s00374-025-01907-3","DOIUrl":"https://doi.org/10.1007/s00374-025-01907-3","url":null,"abstract":"<p>How photosynthetic carbon input regulates the microbial processes involved in carbon incorporation into soil organic carbon (SOC) and its stabilization during grassland degradation remains unclear. We utilized <sup>13</sup>C to trace photosynthetic carbon incorporation into SOC and its pools, particulate (POC) and mineral-associated (MAOC) organic carbon, and carbon assimilation by soil microbes across five stages of alpine grassland degradation (S0, without grazing; S1, moderate grazing; S2–S4, light, moderate, and heavy degradation). As grassland approached S4, SOC in the top layer decreased by 53% compared with that in S3. A similar trend was observed in the middle and bottom soil layers, corresponding to a significant decrease in POC (decreased by 54, 40, and 35% in the top, middle and bottom layer, respectively) and carbon incorporation into POC (decreased by 83, 24, and 91% in the top, middle and bottom layer, respectively). A rapid decrease in MAOC was observed in S4, and carbon incorporation into MAOC decreased abruptly in the middle (10–20 cm) and bottom (20–30 cm) soil layers of S3. More than 57% of the incorporated carbon was concentrated in the top (0–10 cm) layers of S0-S4, whereas the middle and bottom layers of S3 and S4 exhibited nearly zero carbon incorporation. During degradation, fungal groups exhibited a downward trend in photosynthetic carbon assimilation, which was associated with their decreasing contribution to carbon incorporation into SOC. However, a relatively high proportion of bacteria participated in carbon assimilation at all soil depths at each stage, suggesting that more bacteria became active in decomposing the original SOC with decreasing carbon input. Our study successfully links aboveground and belowground processes which are crucial to comprehensively understand ecosystem responses to climate change and human activities.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"22 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adenylate energy charge (AEC) in soil: an almost ignored determination of soil microbial activity - in memory of Phil Brookes
IF 6.5 1区 农林科学
Biology and Fertility of Soils Pub Date : 2025-03-17 DOI: 10.1007/s00374-025-01908-2
Rainer Georg Joergensen, Paolo Nannipieri
{"title":"Adenylate energy charge (AEC) in soil: an almost ignored determination of soil microbial activity - in memory of Phil Brookes","authors":"Rainer Georg Joergensen, Paolo Nannipieri","doi":"10.1007/s00374-025-01908-2","DOIUrl":"https://doi.org/10.1007/s00374-025-01908-2","url":null,"abstract":"<p>The current opinion paper aims to revitalize the important methodological approach initiated by Phil Brookes to measure the adenylate energy charge (AEC) of soils, which indicates the energy status of microbial biomass carbon (MBC). Even dormant soil microorganisms maintain high AEC, i.e., (ATP + 0.5 × ADP) / (ATP + ADP + AMP), levels and, thus, rather stable ATP/MBC and adenylate/MBC ratios. New extractants, improved enzymatic tests, and chromatographic systems are available now that could give new impetus to the measurement of adenylates and AEC in soil. The AEC is a useful tool to investigate important and still unsolved questions in soil microbial biochemistry. For example, drying and rewetting cycles of soil lead to AEC fluctuations, where the energy fluxes of ATP hydrolysis and synthesis remain unknown. Decreasing AEC values might give insights into microbial death processes, particularly in combination with amino sugar assays or molecular genetic techniques.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"58 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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