Biology and Fertility of Soils最新文献

{"title":"Microbiome dynamics of soils covered by plastic and bioplastic mulches","authors":"Giorgia Santini, Maraike Probst, María Gómez-Brandón, Carla Manfredi, Maria Teresa Ceccherini, Giacomo Pietramellara, Lucia Santorufo, Giulia Maisto","doi":"10.1007/s00374-023-01781-x","DOIUrl":"https://doi.org/10.1007/s00374-023-01781-x","url":null,"abstract":"<p>In recent decades, the use of plastic mulch in agriculture has largely increased to meet the growing demand for food. Despite their potential benefits, it is still unknown the long-term impact of mulches on soil microbiome. In this study, we compared at a mesocosm level the effects of polyethylene (Plastic) and Mater-bi® (Bioplastic) mulches on the soil physico-chemical (i.e., pH, soil water content, Fourier transform infrared-attenuated total reflection-FTIR), microbiological, and biochemical (i.e., microbial respiration, enzymatic activities, abundances and composition of bacterial, fungal and microarthropod communities) properties after 6 and 12 months. The analysis of the microbiome revealed an increase in bacterial richness and diversity in the 12-month-treated bioplastic soils. Members of <i>Solirubrobacterales</i>, <i>Vicinamibacterales</i>, <i>Nitrososphaerales</i>, <i>Crenarchaeota</i>, and <i>Clostridiales</i> were the most abundant following the bioplastic treatment. While the fungal and microarthropod communities varied over time, neither of them was affected by the type of mulching. Further longer-term research is needed to clarify the impact of bioplastic and plastic mulches on the soil microbiome, including microarthropods, and its dynamics over time.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138582643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Philip Charles Brookes, 23 March 1951 to 28 September 2023","authors":"R. Joergensen, Kazuyuki Inubushi, Giancarlo Renella","doi":"10.1007/s00374-023-01786-6","DOIUrl":"https://doi.org/10.1007/s00374-023-01786-6","url":null,"abstract":"","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139001887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Competition for nitrogen between plants and microorganisms in grasslands: effect of nitrogen application rate and plant acquisition strategy","authors":"Li Zhang, Jie Liu, Jiazhen Xi, Rui Pang, Anna Gunina, Shurong Zhou","doi":"10.1007/s00374-023-01782-w","DOIUrl":"https://doi.org/10.1007/s00374-023-01782-w","url":null,"abstract":"<p>Several studies have investigated how nitrogen (N) addition changes N competition between focal plant species and soil microorganisms; still, the impact on community-level plant-microbial N competition and the underlying mechanisms remain unclear. We conducted a short-term (4 h) ¹⁵N labeling experiment in an alpine meadow subjected to 7 years of NH₄NO₃ additions (0, 5, 10, and 15 g N m⁻² year⁻¹), by monitoring changes in soil properties (e.g., pH, Al³⁺, NH₄⁺, NO₃⁻), microbial biomass (MB), plant community composition, root traits (e.g., root length, root area, specific root length), as well as the plant (nine focal species and at the community level) and microbial N uptake. Change in the N competition between the nine focal plant species and microorganisms following N addition depended on the species. At the community level, the N addition rate did not affect plant-microbial competition for NH₄⁺ and NO₃⁻ (<i>P</i> &gt; 0.05). Nitrogen addition directly decreased NH₄⁺ competition (<i>β</i> = − 0.700) but indirectly increased because of improved plant uptake due to increased N availability (<i>β</i> = 1.214). Competition for NO₃⁻ was dependent on microbial uptake (<i>β</i> = − 0.953) and was influenced by opposing effects of increased N availability (<i>β</i> = 1.342) and reduced MB (<i>β</i> = − 0.439). Thus, the effects of increased soil N availability and suppressed MB on plant and microbial N competition offset each other, while the plant community had a negligible impact. Such responses should be taken into account for better predictions of the effect of N addition on net primary productivity and ecosystem stability.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138550641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type I dominated methane oxidation and assimilation in rice paddy fields by the consequence of niche differentiation","authors":"Shengmeng Zheng, Shaohong Deng, Chong Ma, Yinhang Xia, Hang Qiao, Jun Zhao, Wei Gao, Qiang Tu, Youming Zhang, Yichao Rui, Jinshui Wu, Yirong Su, Xiangbi Chen","doi":"10.1007/s00374-023-01773-x","DOIUrl":"https://doi.org/10.1007/s00374-023-01773-x","url":null,"abstract":"<p>Conventional aerobic methanotrophs oxidize methane (CH₄) and covert CH₄-derived carbon (C) into biomass at the oxic-anoxic interface of inundated rice paddy fields, playing indispensable role in mitigating greenhouse gas emissions and loss of organic C from methanogenesis. Two phylogenetically distinct groups of methanotrophs, type I (γ-proteobacteria) and type II (α-proteobacteria) methanotrophs, often co-exist in rice paddy soil and compete for CH₄ biotransformation. Since these two methanotrophic groups also possess differential kinetics of CH₄ oxidation and pathways of C assimilation, the consequence of their niche differentiation and metabolic differences in soil is expected to affect the CH₄ oxidation rate and C conversion efficiency. Here, we examined the microbiology, chemistry, and CH₄ metabolism in 24 geographically different paddy soils, covering four climate zones of eastern China. High-throughput sequencing of <i>pmoA</i> gene displayed a clear separation of in situ methanotrophic compositions between temperate (warm and mid-temperate) and warmer (subtropics and tropics) climate zones, likely driven by soil pH. Both methanotrophic groups were detected in soils but proportions of type I methanotrophs increased in temperate soils of higher pH (accounting for 76.1 ± 12.4% and 44.1 ± 14.8% in warm temperate and mid-temperate, respectively). Type II methanotrophs prevailed in warmer zones (accounting for 66.2 ± 21.6% and 70.5 ± 12.1% in tropics and subtropics, respectively) where soils were more acidic. Higher incorporation of ¹³C for synthesis in C₁₄+C₁₆ PLFAs (63.1–93.4% of total production of ¹³C-PLFAs) was found based on microcosm incubation, reflecting type I methanotrophs dominated the CH₄ assimilation in paddy soils. Particularly, temperate soils with increased proportions of type I methanotrophs showed higher CH₄ oxidation rate and C conversion efficiency. Collectively, this study depicts a continental-scale disparity of methanotrophic dynamics that tightly associates with consequence of niche differentiation of different types of methanotrophs and highlights the importance of microbiological control to maximize the rate and efficiency of methanotrophy.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138468862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 5-and a-half-year-experiment shows precipitation thresholds in litter decomposition and nutrient dynamics in arid and semi-arid regions","authors":"Hao Qu, Eduardo Medina-Roldán, Shaokun Wang, Xujun Ma, Xinyuan Wang, Xia Tang, Liangxu Liu","doi":"10.1007/s00374-023-01779-5","DOIUrl":"https://doi.org/10.1007/s00374-023-01779-5","url":null,"abstract":"<p>Precipitation is a key driver of litter decomposition in arid/semiarid regions; where soils are poor in organic matter, and thus re-incorporation of litter is key for soil nutrient accumulation and soil structure. It remains unclear, though, whether litter decomposition responds symmetrically to precipitation variation (e.g., if precipitation surpluses produce a stimulatory effect of a similar magnitude, but opposite direction to inhibitory effects of precipitation deficits), and whether litter decomposition and litter nutrient dynamics in arid and semiarid ecosystems that differ in climate show similar responses to precipitation. We set up a 5-and-a-half-year experiment that manipulated rainfall along a gradient (7 treatments): increases by 20%, 40%, and 60%, background precipitation, and reductions by the same 3 percentages. We applied such experiment in two sites with different pattens of precipitation (Urat: arid; and Naiman: semiarid) in Inner Mongolia to elucidate our questions. Litter mass loss and all nutrients that we measured (carbon, nitrogen, phosphorous, potassium, plus lignin) decomposed faster at the highest level of surplus precipitation, and more slowly in the two largest precipitation reductions. This indicates that these levels of precipitation constitute thresholds (value of precipitation beyond which ecosystem function is critically altered). Litter decomposition in the semiarid site was faster and more complete, but decomposition in the direr Urat was more efficient per unit cumulative rainfall. Thus, site specific effects played an important role in decomposition. Reductions in precipitation decreased the loss of C, N, P, K, and lignin from litter; and clear precipitation thresholds in the dynamic of these nutrients in litter were observed. Overall, this indicated the importance of precipitation limitation at controlling nutrient release. Our study highlights the importance of long-term studies on litter decomposition in environments with slow decomposition rates, and the importance of taking into account mechanistic effects of water availability on decomposition.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138442096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Native soil origin influences the symbiotic N fixation effectiveness of chickpea mesorhizobia grown in Australian soils","authors":"JR Rathjen, M Zaw, MH Ryder, Y Zhou, TV Lai, MD Denton","doi":"10.1007/s00374-023-01780-y","DOIUrl":"https://doi.org/10.1007/s00374-023-01780-y","url":null,"abstract":"<p>Experiments conducted under controlled conditions can be poor predictors of the field performance of rhizobial inoculants. In this study, five field experiments were conducted over 2 years to evaluate the symbiotic performance of 12 previously identified strains isolated from Australia and Myanmar soils that had potential to improve chickpea productivity through symbiotic N₂ fixation. Strains collected from Australian soils had more than double the survival on seed and up to three times the nodulation at some experimental sites, compared with strains isolated from Myanmar soils. Generally, the newly isolated strains did not perform better than the current Australian commercial strain, <i>Mesorhizobium ciceri</i> CC1192. Although Myanmar strains had poor nodulation of chickpea plants (below nodule rating 1 in most cases) under Australian field conditions, the plant traits related to growth and symbiosis, such as shoot dry weight (SDW), yield and N fixation, were improved and sometimes equal to the plants inoculated with the Australian strains. Partial correlations showed that plants inoculated with Myanmar strains had greater associations with N fixation measurements (7 plant traits) than nodule number (1 trait), while a symbiotic effectiveness measure of the ratio between N fixation and nodule mass indicated that Myanmar strains are more than 75% more symbiotically efficient compared with the Australian strains. Better seed and soil survival of the Myanmar strains may increase plant nodulation and may lead to a highly effective inoculant strain. This study is one of the first to report increased symbiotic efficiency of N fixation of novel strains compared to a widely utilised commercial chickpea-nodulating strain, on a per nodule basis.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138442193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal fungi with contrasting life-history strategies differently affect health-promoting compounds in field-grown tomato by changing arbuscule occurrence and mycorrhizal assemblages in roots","authors":"Elisa Pellegrino, Myriam Arcidiacono, Alessandra Francini, Laura Ercoli","doi":"10.1007/s00374-023-01778-6","DOIUrl":"https://doi.org/10.1007/s00374-023-01778-6","url":null,"abstract":"<p>Contrasting life-history characteristics of arbuscular mycorrhizal (AM) fungal families may have important implications for mycorrhizal functioning. Nevertheless, the effect of inoculation with AM fungi having different life-history strategies on the quality parameters of tomato fruits was not investigated. In this study, fruit and sauce quality of two tomato varieties were evaluated in field conditions after inoculation with four AM fungal species belonging to Glomeraceae and Gigasporaceae. The functional relationship between AM fungal traits (i.e., root colonization structures, community diversity) and fruit quality parameters was analyzed. AM fungal inoculation increased total phenols (TPC) and lycopene concentration in fruits of both varieties (47% and 247%, respectively) and antioxidant activity in var. Rio Grande (85%). Gigasporaceae were more effective in increasing TPC and antioxidant activity compared to Glomeraceae in var. Rio Grande. <i>Gigaspora gigantea</i> outperformed <i>Scutellospora pellucida</i> in var. Pisanello for TPC, antioxidant activity, and lycopene<i>.</i> Inoculated strains of <i>G. gigantea</i>, <i>S. pellucida</i>, <i>Funneliformis mosseae</i>, and <i>Sclerocystis sinuosa</i> were molecularly retrieved within tomato roots. In both varieties, a functional relationship between occurrence of arbuscules in roots and fruit quality was found. In var. Rio Grande, the abundance of some native AM fungal taxa shaped the pattern of fruit quality parameters. Gigasporaceae might be of great relevance for the synthesis of health-promoting compounds in tomato and should be included in biostimulant programmes targeting the production of high-quality vegetables.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional players involved in the distinct nitrogen metabolism in two geographically different paddy soils","authors":"Qiaoyu Wu, Yiming Ma, Xinhui Wang, Siyu Yu, Xu Zhao, Xiaoyuan Yan, Xiaojun Zhang","doi":"10.1007/s00374-023-01776-8","DOIUrl":"https://doi.org/10.1007/s00374-023-01776-8","url":null,"abstract":"<p>Black paddy soil (BP) and gleyed paddy soil (GP) are typical rice-planting soils distributed in two different climatic zones in China. The nitrogen utilization efficiency (NUE) of rice growing on the BP was higher than that in GP even when the soils were transplanted to the same location and subjected to the same agricultural management. In this study, we aimed to explore the microbial mechanisms underlying the difference in nitrogen transformation between the two types of soils. The microcosm experiments using BP and GP were performed under different aeration and temperature conditions with controlled ammonium or nitrate. The results indicated that the nitrification of GP was stronger than that of BP, which was associated with the higher relative abundance of ammonium-oxidizing genes in GP than in BP and a group of specialized ammonium-oxidizing species in GP. It indicated that GP had more vigorous nitrifiers, which is not conducive to the nitrogen utilization of ammonium-preferring rice due to faster ammonium consumption. Moreover, more nitrate was consumed in BP than in GP owing to the higher relative abundance of <i>narG</i> and carbon fixation gene <i>accA</i> in BP. Simultaneously, the overall N₂O accumulation in BP was obviously higher than that in GP, which was associated with higher relative abundance of <i>narG</i>, <i>nirK</i>, and <i>norB</i> and the lower relative abundance of <i>nosZ</i>, as well as some specific <i>norB</i> species enriched in BP. These findings advance our understanding of the microbial mechanism of distinct nitrogen metabolism driving the different NUEs in two types of paddy soils.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Converting acidic forests to managed plantations reduces soil nitrogen loss by inhibiting autotrophic nitrification while inducing nitrate immobilization in the tropics","authors":"Qilin Zhu, Ahmed S. Elrys, Lijun Liu, Yunxing Wan, Ruoyan Yang, Jinxia Mou, Yunzhong Chen, Yuqin Wang, Juan Liu, Tongbin Zhu, Yanzheng Wu, Shuirong Tang, Lei Meng, Jinbo Zhang, Christoph Müller","doi":"10.1007/s00374-023-01777-7","DOIUrl":"https://doi.org/10.1007/s00374-023-01777-7","url":null,"abstract":"<p>Soil gross nitrogen (N) transformation rates are highly sensitive to land use change. However, understanding the effect of land use change on internal N cycling patterns and its underlying mechanisms in tropical soils remains elusive. Here, four typical land uses including forest (&gt; 400 years), eucalyptus (15 years), rubber (35 years), and paddy field (40 years) plantations in tropical region of China were investigated. The technique of ¹⁵N tracing was used to quantify soil gross N transformation rates. We also measured soil biochemical properties as well as carbon (C) and N fractions to evaluate the controls on any changes in soil N cycling processes. We found that converting natural tropical forests to managed ecosystems shifts the soil N dynamics from nitrate-dominated N forms towards ammonium-dominated N forms, suggesting that managed ecosystems becoming conservative (i.e., lower ratio of autotrophic nitrification (<i>O</i>_<i>NH4</i>) to ammonium immobilization (<i>I</i>_<i>NH4</i>) and nitrous oxide (N₂O) emissions and higher nitrate immobilization) than the natural tropical forest. The higher tendency of N loss (i.e., higher <i>O</i>_<i>NH4</i><i>/I</i>_<i>NH4</i> and N₂O emissions) of the natural tropical forest was mainly due to the higher concentrations of soil total N and hydrolysable ammonium N and microbial biomass, which stimulated <i>O</i>_<i>NH4</i>. Lower microbial biomass, hydrolysable ammonium N, particulate organic C, and gross N mineralization, however, significantly decreased <i>O</i>_<i>NH4</i> in managed ecosystems. Our study also showed a pivotal role of soil C and N fractions in controlling soil heterotrophic nitrification, which enhanced significantly with decreasing amino sugar N, amino acid N, dissolved organic C, easily oxidizable organic C, and light fraction organic C. Our findings highlighted the pivotal role of soil C and N fractions in regulating soil N cycling under future land use changes.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"109126863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil pH differently affects N2O emissions from soils amended with chemical fertilizer and manure by modifying nitrification and denitrification in wheat-maize rotation system","authors":"Gong Wu, Fei Liang, Qi Wu, Xiao-Gang Feng, Wen-ding Shang, Hua-wei Li, Xiao-xiao Li, Zhao Che, Zhao-rong Dong, He Song","doi":"10.1007/s00374-023-01775-9","DOIUrl":"https://doi.org/10.1007/s00374-023-01775-9","url":null,"abstract":"<p>Emissions of nitrous oxide (N₂O), a potent greenhouse gas, from farmland have been recognized to be affected by soil pH and nitrogen (N) fertilizer application. However, the interactive effects of soil pH and N fertilizer type on N₂O emissions and their influencing mechanism are poorly understood. A field experiment was conducted to elucidate the impacts of synthetic fertilizer and manure on soil properties and N₂O fluxes along a soil acidity gradient (soil pH = 6.8, 6.1, 5.2, and 4.2) in the Huai River Basin, and a lab incubation experiment was performed to understand the underlying mechanisms of changed N₂O flux. Low soil pH inhibited the ammonia-oxidizing bacteria abundance and thereby reduced the N₂O production by nitrification under both synthetic fertilizer and manure application. The N₂O production by denitrification was also reduced with declining soil pH, likely due to the decreased <i>nirS</i> and <i>nirK</i> abundances, and lower NO₃⁻. However, low soil pH reduced the <i>nosZ</i> abundance and increased (<i>nirS</i> + <i>nirK</i>)/<i>nosZ</i> ratio, resulting in the increased N₂O/(N₂O + N₂) ratio. Finally, with the decreased nitrification and denitrification, soil N₂O emission was significantly reduced with declining soil pH regardless of fertilizer types. Compared with synthetic fertilizer, manure application increased soil nutrients (total N, dissolved organic C, and NO₃⁻), <i>nirK</i> abundance, and (<i>nirS</i> + <i>nirK</i>)/<i>nosZ</i> ratio in the soils with pH of 5.2 and 4.2, thereby promoting N₂O production by denitrification and N₂O/(N₂O + N₂) product ratio in acidic soils. Consequently, soil N₂O emission was increased with manure application in acidic soils. This study provides novel insight and improves our understanding of how soil pH regulates nitrification, denitrification, and N₂O emissions from soils amended with chemical fertilizer and manure, which gives guidance on developing N management strategies for sustainable production and N₂O mitigation in acid soils.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71507473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}