Biology and Fertility of Soils最新文献

{"title":"Assessment of phosphorus use and availability by contrasting crop plants in a tropical soil","authors":"Lenir Fátima Gotz, Adila Natália França de Almeida, Rafael de Souza Nunes, Leo Murtagh Condron, Paulo Sergio Pavinato","doi":"10.1007/s00374-024-01833-w","DOIUrl":"https://doi.org/10.1007/s00374-024-01833-w","url":null,"abstract":"<p>Phosphorus (P) is a key element for energy transfer, and biosynthesis of nucleic acids and cell membranes. The objective of this study was to investigate and quantify P utilization by different grain—maize (<i>Zea mays</i> L.) and soybean (<i>Glycine max</i> L.)—and forage-cover crop brachiaria (<i>Brachiaria ruziziensis</i>) plant species in a low fertility highly weathered Oxisol. Two rates of P (25 and 50 mg kg⁻¹) were applied by water-soluble P fertilizer (triple superphosphate) to each of 12 crop cycles, together with a control (no P added). Measurements included plant biomass production and P uptake for each cycle, and analysis of soil P fractions (including labile and non-labile) and enzymes activities (acid phosphatase and β-glucosidase) were done at the beginning of the experiment and after 3, 6, and 12 cycles. Total biomass production and P uptake/removal were significantly higher for brachiaria than maize and soybean, which was reflected in the P use efficiency (PUE), being higher for brachiaria (57%), compared with maize (26%) and soybean (21%). The higher PUE by brachiaria was partly attributed to higher levels of acid phosphatase and β-glucosidase activities which indicated enhanced biological activity and P cycling under brachiaria. Data from the control treatment clearly demonstrated that all three plant species mobilized stable/occluded fractions of P throughout the experiment, however, brachiaria could produce more using less P. The findings of this study indicated the inclusion of brachiaria in crop rotations as a forage or cover crop/green manure may enhance overall P use efficiency.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"44 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141079380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial ammonium immobilization promoted soil nitrogen retention under high moisture conditions in intensively managed fluvo-aquic soils","authors":"Hui Wang, Zhifeng Yan, Zengming Chen, Xiaotong Song, Jinbo Zhang, Si-Liang Li, Christoph Müller, Xiaotang Ju, Xia Zhu-Barker","doi":"10.1007/s00374-024-01831-y","DOIUrl":"https://doi.org/10.1007/s00374-024-01831-y","url":null,"abstract":"<p>Quantifying the gross rates of individual nitrogen (N) processes is critical for understanding the availability, retention and loss of N and its eco-environmental impacts in agricultural ecosystems. Here, we carried out a ¹⁵N tracing study to quantify the influence of soil moisture on the gross rates of ten different N processes in two intensively managed fluvo-aquic soils. Results showed that the gross N mineralization rates were insensitive to changes in soil moisture, ranging from 40 to 120% water-filled pore space (WFPS). Contrarily, the gross ammonium (NH₄⁺) immobilization rates increased exponentially with elevated soil moisture. Specifically, under high soil moisture conditions (i.e., 90–120%WFPS), the gross NH₄⁺ immobilization rates (4.04 ± 0.83 and 0.88 ± 0.28 mg N kg^− 1d^− 1 for the two soils, respectively) were nearly four times higher than those under medium or low moisture conditions (i.e., 40–80%WFPS). Meanwhile, the high WFPS reduced the gross autotrophic nitrification rates (5.92 ± 2.15 and 12.31 ± 3.83 mg-N kg^− 1d^− 1 for the two soils, respectively) to only one-third to one-half of those that were observed under medium or low WFPS. By contrast, the rates of nitrate (NO₃⁻) immobilization increased in one soil whereas they decreased in another under high moisture conditions, and the other N processes (including heterotrophic nitrification and dissimilatory nitrate reduction to ammonium (DNRA)) were negligible throughout the different WFPS. Overall, our results suggest that under highly saturated conditions, the increase in microbial NH₄⁺ immobilization and decrease in autotrophic nitrification are critical for N retention in the fluvo-aquic soils. These findings provide valuable insights into potential alterations in soil N retention or loss under future climate change scenarios, where more intensive irrigation and extreme rainfall events are anticipated.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"08 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140919639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of volatile emissions in olive trees: sustained effect of Trichoderma afroharzianum T22 on induced plant defenses after simulated herbivory","authors":"Martin Aguirrebengoa, Beatriz Moreno, Rafael Alcalá-Herrera, Rafael Núñez, Nuria Guirado, Juan M. García, María J. Pozo, Emilio Benítez","doi":"10.1007/s00374-024-01830-z","DOIUrl":"https://doi.org/10.1007/s00374-024-01830-z","url":null,"abstract":"<p>We explored the activation of defense genes and the changes in volatile profiles in olive (<i>Olea europaea</i> var. Picual) plants subjected to mechanical wounding and prior soil inoculation with the fungus <i>Trichoderma afroharzianum</i> T22. Our findings indicate a sustained effect of the inoculant in olive plants, which shifted the constitutive volatile emission more significantly towards an aldehyde-dominated blend than the mechanical damage alone. Furthermore, we found that wounding alone did not alter the expression of hydroperoxide lyase genes associated with aldehyde biosynthesis. However, this expression was significantly enhanced when combined with prior T22 inoculation. Mechanical wounding amplified the plant’s immediate defensive response by enhancing the upregulation of the direct defense enzyme acetone cyanohydrin lyase. <i>Trichoderma afroharzianum</i> T22 also modulated direct defense, although to a lesser extent, and its effect persisted 9 months after inoculation. Metagenomic analyses revealed that aerial mechanical damage did influence specific root bacterial functions. Specifically, an upregulation of predicted bacterial functions related to various metabolic processes, including responses to biotic and abiotic stresses, was observed. On the contrary, T22’s impact on bacterial functional traits was minor and/or transient.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"6 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140919724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fates of slurry-nitrogen applied to mountain grasslands: the importance of dinitrogen emissions versus plant N uptake","authors":"Michael Dannenmann, Irina Yankelzon, Svenja Wähling, Elisabeth Ramm, Mirella Schreiber, Ulrike Ostler, Marcus Schlingmann, Claus Florian Stange, Ralf Kiese, Klaus Butterbach-Bahl, Johannes Friedl, Clemens Scheer","doi":"10.1007/s00374-024-01826-9","DOIUrl":"https://doi.org/10.1007/s00374-024-01826-9","url":null,"abstract":"<p>Intensive fertilization of grasslands with cattle slurry can cause high environmental nitrogen (N) losses in form of ammonia (NH₃), nitrous oxide (N₂O), and nitrate (NO₃⁻) leaching. Still, knowledge on short-term fertilizer N partitioning between plants and dinitrogen (N₂) emissions is lacking. Therefore, we applied highly ¹⁵N-enriched cattle slurry (97 kg N ha⁻¹) to pre-alpine grassland field mesocosms. We traced the slurry ¹⁵N in the plant-soil system and to denitrification losses (N₂, N₂O) over 29 days in high temporal resolution. Gaseous ammonia (NH₃), N₂ as well N₂O losses at about 20 kg N ha⁻¹ were observed only within the first 3 days after fertilization and were dominated by NH₃. Nitrous oxide emissions (0.1 kg N ha⁻¹) were negligible, while N₂ emissions accounted for 3 kg of fertilizer N ha⁻¹. The relatively low denitrification losses can be explained by the rapid plant uptake of fertilizer N, particularly from 0–4 cm depth, with plant N uptake exceeding denitrification N losses by an order of magnitude already after 3 days. After 17 days, total aboveground plant N uptake reached 100 kg N ha⁻¹, with 33% of N derived from the applied N fertilizer. Half of the fertilizer N was found in above and belowground biomass, while at about 25% was recovered in the soil and 25% was lost, mainly in form of gaseous emissions, with minor N leaching. Overall, this study shows that plant N uptake plays a dominant role in controlling denitrification losses at high N application rates in pre-alpine grassland soils.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"121 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140902885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil contribution to the cobalamin (vitamin B12) supply of terrestrial organisms","authors":"Arne Matteo Jörgensen, Rainer Georg Joergensen","doi":"10.1007/s00374-024-01828-7","DOIUrl":"https://doi.org/10.1007/s00374-024-01828-7","url":null,"abstract":"<p>Cobalamin (Vitamin B₁₂) is a cofactor for many enzymes, including those in bacteria, archaea, algae, and mammals. In humans, cobalamin deficiency can lead to pernicious anaemia as well as gastrointestinal and neurological disorders. In contrast to marine ecosystems, there is a great paucity of information on the role of soils and terrestrial plants in the supply of cobalt and cobalamin to microorganisms and animals. The content of cobalt cations in most soils is usually sufficient to maintain growth, and the density of cobalamin-producing soil prokaryotes is high in comparison to water bodies. The cobalt content of most soils is usually sufficient in comparison with water, and the density of cobalamin-producing soil prokaryotes is high. Therefore, terrestrial plants are an important cobalt source for cobalamin-producing rumen and gut prokaryotes. The major source of cobalamin for most other animals is the meat of ruminants as well as other animal-derived products, bacteria in insects, and coprophagy, e.g., by rodents. In addition, faecal deposits, and fertilizers as well as soil bacteria add to the cobalamin supply. However, those archaea and bacteria that do not produce cobalamin obtain this coenzyme or its analogues from the environment. Therefore, presence or absence of cobalamin-producing species in soil affects the whole soil microbiome. However, our knowledge concerning microbial producers and consumers of cobalamin in soils is still limited, despite some recent advances. The main reasons are a low cobalamin content in soils and challenging methods of determination. In this regard, advanced analytical knowledge and technical equipment are required, which are usually unavailable in soil laboratories. This review provides relevant methodological information on sample homogenization, extraction, concentration, and purification as well as analysis of cobalamin.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"20 5 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140895380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root mucilage nitrogen for rhizosphere microorganisms under drought","authors":"Meisam Nazari, Samuel Bickel, Yakov Kuzyakov, Nataliya Bilyera, Mohsen Zarebanadkouki, Birgit Wassermann, Michaela A. Dippold","doi":"10.1007/s00374-024-01827-8","DOIUrl":"https://doi.org/10.1007/s00374-024-01827-8","url":null,"abstract":"<p>Nitrogen (N) is a crucial nutrient for the growth and activity of rhizosphere microorganisms, particularly during drought conditions. Plant root-secreted mucilage contains N that could potentially nourish rhizosphere microbial communities. However, there remains a significant gap in understanding mucilage N content, its source, and its utilization by microorganisms under drought stress. In this study, we investigated the impact of four maize varieties (DH02 and DH04 from Kenya, and Kentos and Keops from Germany) on the secretion rates of mucilage from aerial roots and explored the origin of mucilage N supporting microbial life in the rhizosphere. We found that DH02 exhibited a 96% higher mucilage secretion rate compared to Kentos, while Keops showed 114% and 89% higher secretion rates compared to Kentos and DH04, respectively. On average, the four maize varieties released 4 μg N per root tip per day, representing 2% of total mucilage secretion. Notably, the natural abundance of ¹⁵N isotopes increased (higher δ¹⁵N signature) with mucilage N release. This indicates a potential dilution of the isotopic signal from biological fixation of atmospheric N by mucilage-inhabiting bacteria as mucilage secretion rates increase. We proposed a model linking mucilage secretion to a mixture of isotopic signatures and estimated that biological N fixation may contribute to 45 - 75% of mucilage N per root tip. The N content of mucilage from a single maize root tip can support a bacterial population ranging from 10⁷ to 10¹⁰ cells per day. In conclusion, mucilage serves as a significant N-rich resource for microbial communities in the rhizosphere during drought conditions.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"10 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140895555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring polyphosphates in soil: presence, extractability, and contribution to microbial biomass phosphorus","authors":"Petr Čapek, Adéla Tupá, Michal Choma","doi":"10.1007/s00374-024-01829-6","DOIUrl":"https://doi.org/10.1007/s00374-024-01829-6","url":null,"abstract":"<p>Polyphosphates (Poly-P) are known to fulfil several important physiological functions. Many microorganisms can accumulate large amounts of Poly-P in their biomass. Regardless of these facts, systematic research on Poly-P in soil is missing, probably due to the absence of any method of direct Poly-P quantification. In this study, we attempted to unequivocally prove the presence of Poly-P in the biomass of soil microorganisms and quantify their extractability and contribution to microbial biomass phosphorus. To do so, we combined several approaches that can indicate Poly-P presence in soil microbial biomass indirectly, i.e. growth of soil inoculum on media without phosphorus, associated with measurement of changes in the microbial biomass stoichiometry, and the colour of the microbial suspension stained by the Neisser method. All soil microbial communities exhibited growth on media without phosphorus. As the growth on this media depleted Poly-P content, the biomass carbon to phosphorus and nitrogen to phosphorus ratio increased and the colour of the microbial suspension stained by the Neisser method changed predictively. The associated Poly-P addition experiment indicated that the recovery of added Poly-P from soil in form of soluble reactive phosphorus in sodium bicarbonate extract may reach up to 93% mainly due to abiotic depolymerization. Using a simple stoichiometric model applied to measured data, we calculated that the Poly-P content of microbial biomass in our soils may be up to 45 or 70% of total microbial biomass phosphorus depending on the assumptions applied regarding parameter values. We discuss the magnitude of error associated with the measurement of soil microbial phosphorus due to the high extractability of Poly-P.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"08 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inoculation and tracking of beneficial microbes reveal they can establish in field-grown potato roots and decrease blemish diseases","authors":"Geoffrey Darbon, Stéphane Declerck, Gaétan Riot, Marcé Doubell, Brice Dupuis","doi":"10.1007/s00374-024-01822-z","DOIUrl":"https://doi.org/10.1007/s00374-024-01822-z","url":null,"abstract":"<p>In a three-year field study, we inoculated two potato varieties with a selection of four beneficial microbial strains (i.e. <i>Rhizophagus irregularis</i> MUCL41833, <i>Trichoderma asperelloides</i> A, <i>Pseudomonas brassicacearum</i> 3Re2-7 and <i>Paraburkholderia phytofirmans</i> PsJN), alone or in combination. Plants were grown under rainfed or irrigated conditions, and potato yield and development of several diseases were evaluated. The microbial inoculants were traced in the root system at different stages of crop development via molecular markers. Whatever the water supply, the inoculants had no effect on yield. Conversely, some of the inoculants were able to lower the incidence and/or severity of several blemish diseases, namely common scab-associated symptoms (CSAS) and silver scurf/black dot-associated symptoms (SSAS). Microbial consortia were more efficient in decreasing symptoms compared to single strain inoculations. The best control was obtained with the combination of <i>R. irregularis</i> and <i>P. brassicacearum</i>, which reduced the incidence of CSAS by 22% and severity of SSAS by 21%. Root tracking revealed that <i>P. brassicacearum</i> and <i>P. phytofirmans</i> PsJN were able to establish in the root system of the potato, while only <i>P. brassicacearum</i> was detected from emergence until flowering of the plants.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"51 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140640094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic data highlight shifted nitrogen regime induced by wetland reclamation","authors":"Kexin Li, Nannan Wang, Fenghui Yuan, Xinhao Zhu, Yunjiang Zuo, Jianzhao Liu, Ziyu Guo, Ying Sun, Rui Su, Lihua Zhang, Sergei Lupakov, Yanyu Song, Changchun Song, Xiaofeng Xu","doi":"10.1007/s00374-024-01820-1","DOIUrl":"https://doi.org/10.1007/s00374-024-01820-1","url":null,"abstract":"<p>Natural wetlands are mostly nitrogen-limited ecosystems, while reclamation stimulates the loss of nitrogen (N) in soils by shifting the N regime. To investigate the microbial mechanisms of the N regime shift, we first conducted a global meta-analysis to quantify the wetland reclamation impacts on soil mineral N pools and then a field campaign to sample 24 soil cores up to 100 cm depth in a natural wetland and a 23-year cultivated soybean field from the Sanjiang Plain in northeastern China. After wetland reclamation, the N regime was shifted to cause a potential risk of massive N loss in soils; their microbial mechanisms were revealed through metagenomic data. In cropland, the relative abundance of genes involved in nitrification and assimilatory nitrate reduction to ammonia (ANRA) were enriched while those in N fixation, mineralization, denitrification, and dissimilatory nitrate reduction to ammonia (DNRA) were diminished. Wetland reclamation substantially enhanced the relative abundance of genes involved in nitrification (except for genes for ammonia oxidation to NH₂OH) and denitrification in surface (0–30 cm) soils but decreased them in subsurface (30–100 cm) soils. After wetland reclamation, the relative abundance of genes involved in denitrification and DNRA significantly reduced in spring and summer, but such patterns were not found in autumn and winter. This change enhanced potential microbial-driven N loss in spring and summer. The metagenomic data serve as surrogate data sources for quantifying soil roles on soil N cycles under land use change.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"1 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140640088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of liming and maize residues on N2O and N2 fluxes in agricultural soils: an incubation study","authors":"Lisa Pfülb, Lars Elsgaard, Peter Dörsch, Roland Fuß, Reinhard Well","doi":"10.1007/s00374-024-01825-w","DOIUrl":"https://doi.org/10.1007/s00374-024-01825-w","url":null,"abstract":"<p>Since it is known that nitrous oxide (N₂O) production and consumption pathways are affected by soil pH, optimising the pH of agricultural soils can be an important approach to reduce N₂O emissions. Because liming effects on N₂O reduction had not been studied under ambient atmosphere and typical bulk density of arable soils, we conducted mesoscale incubation experiments with soils from two liming trials to investigate the impact of long-term pH management and fresh liming on N transformations and N₂O production. Soils differed in texture and covered a range of pH levels (3.8–6.7), consisting of non-limed controls, long-term field-limed calcite and dolomite treatments, and freshly limed soils. Both soils were amended with ¹⁵N-labelled potassium nitrate (KNO₃) and incubated with and without incorporated maize litter. Packed soil mesocosms were cycled through four phases of alternating temperatures and soil moistures for at least 40 days. Emissions of N₂O and dinitrogen (N₂) as well as the product ratio of denitrification N₂O/(N₂O + N₂), referred to as N₂Oi were measured with the ¹⁵N gas flux method in N₂-reduced atmosphere. Emissions of N₂O increased in response to typical denitrifying conditions (high moisture and presence of litter). Increased temperature and soil moisture stimulated microbial activity and triggered denitrification as judged from ¹⁵NO₃⁻ pool derived N₂O + N₂ emissions. Fresh liming increased denitrification in the sandy soil up to 3-fold but reduced denitrification in the loamy soil by 80%. N₂Oi decreased throughout the incubation in response to fresh liming from 0.5–0.8 to 0.3–0.4, while field-limed soils had smaller N₂Oi (0.1–0.3) than unlimed controls (0.9) irrespective of incubation conditions. Our study shows that the denitrification response (i.e., N₂O + N₂ production) to liming is soil dependent, whereas liming effects on N₂Oi are consistent for both long- and short-term pH management. This extends previous results from anoxic slurry incubation studies by showing that soil pH management by liming has a good mitigation potential for agricultural N₂O emissions from denitrification under wet conditions outside of cropping season.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"2 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140637634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}