Biology and Fertility of Soils最新文献_第9页

Microbial nitrification inhibitor enhances alpine grassland productivity through narrowing plant niche breadth by utilizing more ammonium 微生物硝化抑制剂利用更多的铵，通过缩小植物生态位宽度来提高高寒草地的生产力

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-04-25 DOI: 10.1007/s00374-025-01917-1

Lizhen Cui, Kai Xue, Junfu Dong, Shun He, Congjia Li, Zhe Pang, Wenjing Liu, Biao Zhang, Shutong Zhou, Kui Wang, Tong Li, Jianqing Du, Xiaoyong Cui, Yanbin Hao, Yanfen Wang

{"title":"Microbial nitrification inhibitor enhances alpine grassland productivity through narrowing plant niche breadth by utilizing more ammonium","authors":"Lizhen Cui, Kai Xue, Junfu Dong, Shun He, Congjia Li, Zhe Pang, Wenjing Liu, Biao Zhang, Shutong Zhou, Kui Wang, Tong Li, Jianqing Du, Xiaoyong Cui, Yanbin Hao, Yanfen Wang","doi":"10.1007/s00374-025-01917-1","DOIUrl":"https://doi.org/10.1007/s00374-025-01917-1","url":null,"abstract":"Stimulated microbial nitrification has been reported during grassland degradation when plants and microbes compete for declined nitrogen (N) resources. However, it remains unclear whether inhibiting microbial nitrification would change such competition and alter grassland productivity. Here, we investigated changes induced by the nitrification inhibitor (NI) application in N acquisition strategies, niche breadth and competitiveness of plants and microbes, with different soil N levels in greenhouse. The 15N labeling technology was employed with ammonium, nitrate and glycine to quantify N uptake. NI significantly (P ≤ 0.02) decreased abundances of AOA and AOB genes for microbial nitrifiers in low-N (1.3 g/kg for total N) soils, and AOB abundance in high-N (1.8 g/kg) soils, validating the efficacy of NI in inhibiting nitrification. NI significantly (P < 0.01) increased the soil ammonium content by 25.50% and 10.43% in low- and high-N soils, respectively. Moreover, NI narrowed the plant niche breadth for N utilization by concentrating more on ammonium uptake in both low- and high-N soils. Consequently, NI significantly (P ≤ 0.04) increased the plant biomass by 10.02% and 10.16% in low- and high-N soils, respectively. In comparison, microbial competitiveness against plants for ammonium decreased by NI in low-N soils, leading to a 23.41% reduction in microbial biomass (P < 0.01); while they remained unchanged in high-N soils. Overall, our study revealed the effectiveness of NI application for enhancing grassland productivity by reducing plant niche breadth through utilizing more ammonium, suggesting a viable strategy to restore degraded grasslands without any external N input.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"259 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyraclostrobin and polyethylene nanoplastics jointly interfere with the antibiotic resistome in earthworm gut 吡咯菌酯和聚乙烯纳米塑料共同干扰蚯蚓肠道抗生素抵抗组

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-04-21 DOI: 10.1007/s00374-025-01916-2

Jing Yang, Conglai Zheng, Enyu Zhang, Guy Smagghe, Shunhua Gui, Xiaomao Wu, Xiangsheng Chen

{"title":"Pyraclostrobin and polyethylene nanoplastics jointly interfere with the antibiotic resistome in earthworm gut","authors":"Jing Yang, Conglai Zheng, Enyu Zhang, Guy Smagghe, Shunhua Gui, Xiaomao Wu, Xiangsheng Chen","doi":"10.1007/s00374-025-01916-2","DOIUrl":"https://doi.org/10.1007/s00374-025-01916-2","url":null,"abstract":"Antibiotic resistance has emerged as a global threat to public health. However, the current information is insufficient to understand how other pollutants, such as fungicides and nanoplastics, affect the spread of antibiotic resistance genes (ARGs) among bacteria in the soil. Here, our findings revealed that polyethylene nanoplastics (PENPs) prolonged the persistence of pyraclostrobin (PYR) in the soil by 13 days, increased PYR bioaccumulation in earthworm (Eisenia fetida) by 8.4%, and reduced its weights by 26.8%. PYR alone or combined with PENPs significantly increased the microbiome diversities of earthworm guts, while PENPs alone decreased those but increased the relative abundances of Proteobacteria and Firmicute. PYR and/or PENPs enhanced the diversity and abundance of ARGs in earthworm guts, the range of ARG hosts, and the complexity of ARGs and antibiotic-resistant bacteria coexistence network. The abundance of plasmid-origin ARG-harboring contigs in PYR, PENP, and PYR + PENP treatments was 1.5-, 3.8-, and 2.4-fold higher than that in the control, respectively. Overall, PYR and/or PENPs specifically disturbed the antibiotic resistome in earthworm guts by altering the bacterial community composition and richness, increasing the abundance of mobile genetic elements (MGEs) and ARGs, and modifying the co-occurrence pattern of ARGs-MGEs, particularly plasmids.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"4 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does an enhanced microbial diversity promote the resistance of soil multifunctionality against drought events in amended soils? 微生物多样性的增强是否促进了改良土壤对干旱事件的多功能性抵抗？

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-04-10 DOI: 10.1007/s00374-025-01914-4

L. Morales-Salmerón, E. Fernández-Boy, B. Herrador, R. León, M. T. Domínguez

{"title":"Does an enhanced microbial diversity promote the resistance of soil multifunctionality against drought events in amended soils?","authors":"L. Morales-Salmerón, E. Fernández-Boy, B. Herrador, R. León, M. T. Domínguez","doi":"10.1007/s00374-025-01914-4","DOIUrl":"https://doi.org/10.1007/s00374-025-01914-4","url":null,"abstract":"A large fraction of the Mediterranean soils is threatened by losses of organic matter and biodiversity, which could compromise the provision of soil ecosystem services and the stability of ecosystems in the face of climate change. In this work we explore several hypotheses related to the role of C inputs and microbial diversity on soil multifunctionality and its resistance to drought in degraded Mediterranean soils. We designed a factorial experiment to test the effect of the addition of an organic amendment and of microbial diversity (using four inoculants with different abundance and diversity of soil microbiota), on the resistance of soil functionality against drought in pot mesocosms. Pots were sown with a forage mixture (Lolium rigidum and Medicago polymorpha), and plant productivity, soil chemical properties, and microbial activity and diversity were measured before and after a simulated drought event. The amendment favored soil moisture, enhancing the stability of the productivity of M. polymorpha. In contrast, the manipulation of inoculation load had a limited effect on the resistance of microbiological activity. Indeed, microbial functioning was highly resistant to reduced water inputs, probably related to the prevalence of Gram positive bacteria. Besides, the effect of microbial diversity on soil multifunctionality was limited. Structural equation modelling confirmed that the enhancement of multifunctionality after soil amendment was attributed to the direct effect of organic C on soil moisture and chemical fertility. In these degraded soils, physico-chemical limitations are the major drivers of soil multifunctionality rather than bacterial or fungal diversity.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"183 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143814262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorus fertilizer enhances the tolerance of rhizosphere microbial community to low-light stress in Tartary buckwheat 施磷肥提高苦荞根际微生物群落对弱光胁迫的耐受性

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-04-08 DOI: 10.1007/s00374-025-01915-3

Yuchuan Zhang, Zhijia Cui, Yujiao Li, Meng Wang, Feifei Zhang, Yu Feng, Xi Zhang, Qinghua Yang, Lixin Tian, Baili Feng

{"title":"Phosphorus fertilizer enhances the tolerance of rhizosphere microbial community to low-light stress in Tartary buckwheat","authors":"Yuchuan Zhang, Zhijia Cui, Yujiao Li, Meng Wang, Feifei Zhang, Yu Feng, Xi Zhang, Qinghua Yang, Lixin Tian, Baili Feng","doi":"10.1007/s00374-025-01915-3","DOIUrl":"https://doi.org/10.1007/s00374-025-01915-3","url":null,"abstract":"Low-light stress has become an important factor limiting crop yield and quality improvement. Appropriate phosphorus (P) addition can enhance soil microbial activity and nutrient availability, thereby alleviating the negative impacts of low-light stress. However, the role of crop rhizosphere microorganisms in the mitigation of low-light stress by P addition in agroecosystems remains unclear. In this study, three light conditions (normal light, S0; moderate low-light stress, S1; severe low-light stress, S2) and three P addition levels (0 kg ha− 1, P0; 35 kg ha− 1, P1; 70 kg ha− 1, P2) were applied to analyze the combined effects on the rhizosphere microbial diversities, compositions, co-occurrence patterns, and assembly mechanisms during critical growth stages (flowering, filling, and maturity stages). The results showed that both low-light stress and P appreciably affected rhizosphere microbial community composition, with P promoting the proliferation of rhizosphere beneficial microbes under low-light stress. Low-light stress reduced rhizosphere microbial α-diversity, and S2 simplified microbial networks. In contrast, P1 increased bacterial network complexity, connectivity, and stability. Indicator taxa analysis revealed that P1 increased the abundance of shared and specific species in rhizosphere microbial networks. Under S1, P1 enhanced keystone taxa abundance. Community assembly analysis indicated that bacterial communities were governed by deterministic processes, whereas low-light stress reduced fungal stochasticity, which was increased by P addition. These findings highlight that P addition under low-light stress can enhance the tolerance of Tartary buckwheat rhizosphere microbial community by modulating rhizosphere microbial diversity, composition, and network stability, providing insights into alleviating low-light stress.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"24 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exudate pulses throughout the entire growth period trigger the increase in maize phosphorus use efficiency by modifying soil keystone microbial taxa 整个生育期的分泌物脉冲通过改变土壤关键微生物类群触发玉米磷利用效率的提高

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-04-04 DOI: 10.1007/s00374-025-01912-6

Guiwei Wang, Yuechao Yang, Yuanyuan Yao, Xiaoqi Wang

{"title":"Exudate pulses throughout the entire growth period trigger the increase in maize phosphorus use efficiency by modifying soil keystone microbial taxa","authors":"Guiwei Wang, Yuechao Yang, Yuanyuan Yao, Xiaoqi Wang","doi":"10.1007/s00374-025-01912-6","DOIUrl":"https://doi.org/10.1007/s00374-025-01912-6","url":null,"abstract":"Exudates are the medium through which plants adapt to complex soil environments, however, the mechanisms of how different types of root exudates increase maize yield and phosphorus (P) use efficiency (PUE) throughout the entire growth period remains unknown. In this study, we designed an experiment to examine the effects of continuous addition of exudate substances on maize growth and P uptake over the entire growth period. The addition of succinic acid, luteolin, and inositol significantly increased maize biomass, particularly root biomass. Specifically, the treatment with added succinic acid increased maize yield by 11.6% and PUE by 8%. Additionally, we found that different exudate substances significantly altered the soil bacterial and fungal communities, thereby increasing soil P bioavailability. The microbial co-occurrence networks revealed that Actinobacteriota and Proteobacteria keystone ASVs, enriched by the addition of succinic acid, luteolin, and inositol, were significantly associated with maize P uptake. Furthermore, at the V12 stage (Twelve leaves unfolded), the addition of exudate substances significantly increased alkaline phosphatase activity, and at the R6 stage (Maturity), soil available P content was significantly elevated, enhancing soil P bioavailability. These findings provide evidence for future exploration of the interaction mechanisms between plants and soil microbes and optimizing nutrient management strategies in farmland.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"183 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil bacterial and fungal communities beneath different forest types differentially and promptly respond to non-catastrophic typhoon disturbance 不同森林类型下土壤细菌和真菌群落对非灾害性台风扰动的响应不同且迅速

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-03-31 DOI: 10.1007/s00374-025-01913-5

Zhihui Wang, Rui Zhang, Rui Cao, Lifeng Wang, Zhuang Wang, Qin Wang, Yuchen Lu, Yong Zhang, Wanqin Yang

{"title":"Soil bacterial and fungal communities beneath different forest types differentially and promptly respond to non-catastrophic typhoon disturbance","authors":"Zhihui Wang, Rui Zhang, Rui Cao, Lifeng Wang, Zhuang Wang, Qin Wang, Yuchen Lu, Yong Zhang, Wanqin Yang","doi":"10.1007/s00374-025-01913-5","DOIUrl":"https://doi.org/10.1007/s00374-025-01913-5","url":null,"abstract":"The immediate responses of soil microbial communities to non-catastrophic typhoon disturbances remain largely unclear, despite soil microorganisms are key contributors to ecosystem functioning and sensitive indicators to forest disturbances. To address this gap, we simultaneously investigated soil microbial communities beneath bamboo forest (BF), Chinese fir forest (CF), secondary broadleaf forest (SF) and mixed forest (MF) in the subtropical mountain area of southeastern China before and after typhoon disturbances. Typhoon disturbances significantly altered the composition of soil microbial communities, decreasing bacterial diversity by 12.3% and network robustness by 22.6% in the topsoil, while increasing fungal diversity by 75.0% and network robustness by 33.2% in the litter layer. Notably, these indices returned to the per-typhoon levels post-disturbance. Meanwhile, typhoon disturbances increased the abundance of functional guild on bacterial cellular processes, fungal dung saprotrophs and plant saprotrophs in the topsoil. Moreover, the immediate responses of microbial community composition and diversity to typhoon disturbances were more pronounced in the CF and SF, and bacterial communities were more responsive in the topsoil, while fungal communities were more responsive in the litter layer. Additionally, soil temperature was the primary driver of microbial communities in the topsoil, while litter cellulose and calcium concentrations were key factors in the litter layer. Besides, carbon and nutrient concentrations were also significantly correlated with the composition of taxa and functional guilds of soil microbial communities. In summary, the immediate responses of microbial communities to typhoon disturbances vary considerably depending on forest types and soil horizon, with rapid recovery following typhoon events.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"72 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of methods for assessing fungi-to-bacteria ratio of soil 土壤真菌细菌比测定方法的比较

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-03-27 DOI: 10.1007/s00374-025-01911-7

Miikka B. Laine, Sami J. Taipale, Marja Tiirola

{"title":"Comparison of methods for assessing fungi-to-bacteria ratio of soil","authors":"Miikka B. Laine, Sami J. Taipale, Marja Tiirola","doi":"10.1007/s00374-025-01911-7","DOIUrl":"https://doi.org/10.1007/s00374-025-01911-7","url":null,"abstract":"Measuring bacterial and fungal biomass may offer insights into agroecosystem health. Nevertheless, few studies have directly compared the ability of different methods to assess the abundance of these two microbial groups and their ratio (F/B ratio). This study compared the ability, precision, and repeatability of three commonly used laboratory methods - phospholipid fatty acid (PLFA) analysis, quantitative PCR (qPCR), and droplet-digital PCR (ddPCR) - alongside a commercially available microbial carbon testing tool (microBIOMETER®), to assess the F/B ratio and microbial abundance in agroecosystem soils. We also reviewed recent literature on common measurement and reporting practices. PLFA and ddPCR provided the most reliable outcomes, with PLFA being the most precise, repeatable, and widely used (81% of reviewed studies). However, significant variability in analytical procedures exists between laboratories, and key details, such as storage conditions, are often underreported. MicroBIOMETER® can offer a low-cost option for assessing total microbial biomass, but did not match PLFA results in determining the F/B ratio. ddPCR offered better precision than qPCR but had a narrower dynamic range. Therefore, optimal approach is to use the two methods in parallel. In conclusion, we recommend future studies adopt PLFA analysis as the primary method for assessing microbial abundance and F/B ratio of soils, as PCR-based measurements are influenced by several unavoidable biases. Furthermore, we suggest improvements to the PLFA method to ensure more reliable comparisons across laboratories. Altogether, our study gives guidelines for improving the monitoring of F/B ratio and microbial abundance in agroecosystems.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"18 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inoculation with in vitro promising plant growth-promoting bacteria isolated from nitrogen-limited boreal forest did not translate to in vivo growth promotion of agricultural plants 从氮限制的北方森林中分离的植物促生长细菌在体外接种不能转化为促进农业植物的体内生长

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-03-25 DOI: 10.1007/s00374-025-01910-8

Tinkara Bizjak-Johansson, Anne Braunroth, Regina Gratz, Annika Nordin

{"title":"Inoculation with in vitro promising plant growth-promoting bacteria isolated from nitrogen-limited boreal forest did not translate to in vivo growth promotion of agricultural plants","authors":"Tinkara Bizjak-Johansson, Anne Braunroth, Regina Gratz, Annika Nordin","doi":"10.1007/s00374-025-01910-8","DOIUrl":"https://doi.org/10.1007/s00374-025-01910-8","url":null,"abstract":"Many yet undiscovered plant growth-promoting bacteria are proposed to be harboured in the nitrogen-limited boreal forest. These bacteria are suggested to increase plant growth not only due to their ability to fix nitrogen but also through other growth-promoting properties. Therefore, this study looked at the plant growth promotion potential of endophytic bacteria isolated from boreal forest conifer Scots pine (Pinus sylvestris) needles. Seven assays were used to measure the potential plant growth-promoting abilities of two newly isolated bacteria in this study and seven additionally selected bacteria isolated in our previous study. The three best-performing bacteria were used, either individually or in a consortium, to assess growth promotion on four common crop species. The greenhouse study included the presence of native soil and seed microbiota and used naturally nutrient-abundant soil. The results showed that while all bacteria were capable of multiple plant growth-promoting properties in the in vitro assays, they did not promote plant growth in the in vivo experiment as inoculated plants had similar or decreased chlorophyll content, root and shoot length and dry biomass compared to control plants. Our results show that bacterial plant growth-promoting potential does not always translate into successful plant growth increase in in vivo conditions and highlight the need for a better understanding of plant-bacteria interaction for the future establishment of successful bacterial bioinoculants.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"41 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143695671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrous oxide fluxes, their sources, and soil microbial communities depend more on carbon availability than long- and short-term phosphorus addition 一氧化二氮通量、其来源和土壤微生物群落更多地取决于碳有效性，而不是长期和短期磷添加

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-03-24 DOI: 10.1007/s00374-025-01909-1

Pauline Sophie Rummel, Anthony Imoudu Oyeogbe, Haitao Wang, Tim Urich, Nicole Wrage-Mönnig

{"title":"Nitrous oxide fluxes, their sources, and soil microbial communities depend more on carbon availability than long- and short-term phosphorus addition","authors":"Pauline Sophie Rummel, Anthony Imoudu Oyeogbe, Haitao Wang, Tim Urich, Nicole Wrage-Mönnig","doi":"10.1007/s00374-025-01909-1","DOIUrl":"https://doi.org/10.1007/s00374-025-01909-1","url":null,"abstract":"Denitrification is the key process leading to production and loss of nitrogen gases from soils. Its main drivers are N availability and soil water content, but interactions with other elements, such as carbon and phosphorus, can also influence N2O formation. So far, robust information on the effects of P and the historical context of P addition on N2O sources remains limited. To address this knowledge gap, we conducted a mesocosm chamber experiment using isotopic approaches to investigate N transformations and N2O sources following P fertilizer addition in soils with varying histories of P fertilization (low and high P). Differences in long-term fertilization affected C, N, and P availability as well as microbial community composition and nutrient cycling processes. Initially, microbes in both soils were C-limited with slightly higher C availability and microbial respiration in high P soils. Low P availability in low P soil did not restrict denitrification. In contrast, long-term P-unfertilized soil had higher N2O losses compared to high P soil, which were further stimulated with P addition. Glucose addition alleviated C limitation and strongly promoted microbial growth and respiration, but did not affect N2O emissions among treatments. Bacterial denitrification and nitrifier denitrification were the main N2O forming processes, while dissimilatory nitrate reduction to ammonium (DNRA) contributed to NO3− reduction, but only slightly to N2O formation.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"20 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing phosphorus availability reduces priming effect by facilitating microbial carbon use efficiency in a subtropical forest soil 提高磷有效性通过促进亚热带森林土壤微生物碳利用效率来降低启动效应

IF 6.5 1区农林科学

Biology and Fertility of Soils Pub Date : 2025-03-21 DOI: 10.1007/s00374-025-01906-4

Quanxin Zeng, Qiufang Zhang, Kongcan Mei, Jiguang Feng, Xiaochun Yuan, Yuanyuan Liu, Min Xu, Hao Sun, Biao Zhu, Yuehmin Chen

{"title":"Increasing phosphorus availability reduces priming effect by facilitating microbial carbon use efficiency in a subtropical forest soil","authors":"Quanxin Zeng, Qiufang Zhang, Kongcan Mei, Jiguang Feng, Xiaochun Yuan, Yuanyuan Liu, Min Xu, Hao Sun, Biao Zhu, Yuehmin Chen","doi":"10.1007/s00374-025-01906-4","DOIUrl":"https://doi.org/10.1007/s00374-025-01906-4","url":null,"abstract":"The mechanisms by which phosphorus (P) availability regulates the priming effect (PE) induced by the addition of leaf litter with different qualities remain unclear. Here, soil samples from a subtropical Pinus massoniana forest were added with/without P and/or high- and low-quality 13C-labeled leaf litter. The samples were then incubated in the laboratory for 75 days to assess the PE, microbial community composition, enzyme activity, and microbial carbon use efficiency (CUE). The results showed that litter addition led to a positive PE. High-quality litter inputs stimulated microbial activity but reduced microbial CUE, resulting in a higher PE intensity. By contrast, the PE exhibited a decrease with P addition. Such finding indicates that strategies for obtaining P, such as microbial decomposition of soil organic matter, may be reduced. The random forest analysis revealed that microbial CUE is the dominant factor regulating PE, accounting for 62% of the variation in PE, and it exhibited a negative effect on PE. Collectively, our findings emphasize that P availability regulates PE by decreasing microbial decomposition and increasing CUE, highlighting its essential role in carbon-climate feedbacks.","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":"26 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0