Yang Yu, Xia Kang, Tianhai Liu, Yong Wang, Jie Tang, Weihong Peng, Francis M. Martin, Hao Tan
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In <i>P. chlororaphis</i>-inoculated morel soil beds, <i>P. chlororaphis</i> colonised both the mycelial surface and ascocarp matrix of <i>M. importuna</i>, increasing the abundance of <i>Morchella</i> in soil and the α-diversity of the soil fungal community. Additionally, <i>P. chlororaphis</i> inoculation decreased the abundance of detrimental <i>P. penicillatus</i> and mitigated the WMD incidence, which correspondingly increased the morel yield. Metagenomics revealed that increasing the pseudomonads in the <i>M. importuna</i> mycosphere altered the functionalities of the <i>M. importuna</i> soil microbiota, enhancing the abundances of genes encoding chitinase and alkaline protease and reducing the abundances of genes encoding glucanase and laccase. Under <i>P. chlororaphis</i> inoculation, pathways associated with pathogenic invasion were under-represented in the soil microbiota. 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引用次数: 0
摘要
利用根瘤微生物群作为生物防治剂是保护植物免受病原体侵害的一种很有前景的策略,但其对真菌宿主的功效尚不确定。本研究调查了绿假丝酵母菌(一种细菌菌株)在减轻青霉酵母菌(Paecilomyces penicillatus)方面的功效,青霉酵母菌是一种土传致病真菌,是导致栽培羊肚菌(如 Mochella importuna)白霉病(WMD)的罪魁祸首。有慢性 WMD 的土壤在接种或未接种 P. chlororaphis 的情况下被用来栽培 M. importuna。在接种了 P. chlororaphis 的羊肚菌土壤床中,P. chlororaphis 在 M. importuna 的菌丝表面和 ascocarp 基质上都有定殖,增加了土壤中 Morchella 的数量和土壤真菌群落的 α-多样性。此外,接种 P. chlororaphis 减少了有害的 P. penicillatus 的数量,降低了 WMD 的发生率,从而相应地提高了羊肚菌的产量。元基因组学显示,增加进口羊肚菌菌丝圈中的假单胞菌改变了进口羊肚菌土壤微生物群的功能,提高了几丁质酶和碱性蛋白酶编码基因的丰度,降低了葡聚糖酶和漆酶编码基因的丰度。在 P. chlororaphis 接种的情况下,与病原体入侵相关的途径在土壤微生物群中的代表性不足。这些结果加深了我们对土壤生态系统中细菌-真菌相互作用的理解,并证明了通过操纵真菌菌丝圈中的微生物群抑制疾病的潜力。这些见解可能会带来创新的方法来对抗真菌病原体,并提高羊肚菌等珍贵真菌作物的健康和产量。
Inoculation of the Morchella importuna mycosphere with Pseudomonas chlororaphis alleviated a soil-borne disease caused by Paecilomyces penicillatus
Utilising the rhizosphere microbiota as a biological control agent is a promising strategy to protect plants against pathogens, although its efficacy in fungal hosts is uncertain. This study investigated the efficacy of Pseudomonas chlororaphis, a bacterial strain, in mitigating Paecilomyces penicillatus, a soil-borne pathogenic fungus responsible for white mould disease (WMD) in cultivated morels, such as Morchella importuna. Soils with chronic WMD, inoculated with or without P. chlororaphis, were utilised for M. importuna cultivation. In P. chlororaphis-inoculated morel soil beds, P. chlororaphis colonised both the mycelial surface and ascocarp matrix of M. importuna, increasing the abundance of Morchella in soil and the α-diversity of the soil fungal community. Additionally, P. chlororaphis inoculation decreased the abundance of detrimental P. penicillatus and mitigated the WMD incidence, which correspondingly increased the morel yield. Metagenomics revealed that increasing the pseudomonads in the M. importuna mycosphere altered the functionalities of the M. importuna soil microbiota, enhancing the abundances of genes encoding chitinase and alkaline protease and reducing the abundances of genes encoding glucanase and laccase. Under P. chlororaphis inoculation, pathways associated with pathogenic invasion were under-represented in the soil microbiota. These results enhance our understanding of bacterial–fungal interactions within soil ecosystems and demonstrate the potential for disease suppression through microbiota manipulation within the fungal mycosphere. These insights may lead to innovative approaches to combat fungal pathogens and enhance the health and productivity of valuable fungal crops such as morels.
期刊介绍:
Biology and Fertility of Soils publishes in English original papers, reviews and short communications on all fundamental and applied aspects of biology – microflora and microfauna - and fertility of soils. It offers a forum for research aimed at broadening the understanding of biological functions, processes and interactions in soils, particularly concerning the increasing demands of agriculture, deforestation and industrialization. The journal includes articles on techniques and methods that evaluate processes, biogeochemical interactions and ecological stresses, and sometimes presents special issues on relevant topics.