Bioscience, Biotechnology, and Biochemistry最新文献_第9页

The DNA binding of plant-specific GROWTH-REGULATING FACTOR transcription factors is stabilized by GRF-INTERACTING FACTOR coactivators. 植物特异性生长调节因子转录因子的 DNA 结合受到 GRF-INTERACTING FACTOR 辅激活因子的稳定。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf016

Shohei Nosaki, Masae Ohtsuka

引用次数: 0

NPD7426 suppresses sterol regulatory element-binding proteins by promoting the degradation of mature SREBP forms. NPD7426 通过促进成熟 SREBP 形式的降解来抑制固醇调节元件结合蛋白。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf012

Manami Kodaka, Yuki Matsunaga, Seiya Terada, Minami Kamei, Tsukasa Suzuki, Yuji Yamamoto, Jun Inoue

引用次数: 0

Investigation of trans-to-cis isomerization of cinnamic acid in Arabidopsis using stable-isotope-labeled cinnamic acid. 利用稳定同位素标记肉桂酸研究拟南芥中肉桂酸的反式-顺式异构化。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf019

Kei Tsuzuki, Taiki Suzuki, Kotaro Nishiyama, Yoshiya Seto

引用次数: 0

Validation of machine learning-assisted screening of PKC ligands: PKC binding affinity and activation. 机器学习辅助筛选PKC配体的验证：PKC结合亲和力和激活。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf008

Jumpei Maki, Asami Oshimura, Yudai Shiotani, Maki Yamanaka, Sogen Okuda, Ryo C Yanagita, Shigeru Kitani, Yasuhiro Igarashi, Yutaka Saito, Yasubumi Sakakibara, Chihiro Tsukano, Kazuhiro Irie

{"title":"Validation of machine learning-assisted screening of PKC ligands: PKC binding affinity and activation.","authors":"Jumpei Maki, Asami Oshimura, Yudai Shiotani, Maki Yamanaka, Sogen Okuda, Ryo C Yanagita, Shigeru Kitani, Yasuhiro Igarashi, Yutaka Saito, Yasubumi Sakakibara, Chihiro Tsukano, Kazuhiro Irie","doi":"10.1093/bbb/zbaf008","DOIUrl":"10.1093/bbb/zbaf008","url":null,"abstract":"Protein kinase C (PKC) is a family of serine/threonine kinases, and PKC ligands have the potential to be therapeutic seeds for cancer, Alzheimer's disease, and human immunodeficiency virus infection. However, in addition to desired therapeutic effects, most PKC ligands also exhibit undesirable pro-inflammatory effects. The discovery of new scaffolds for PKC ligands is important for developing less inflammatory PKC ligands, such as bryostatins. We previously reported that machine learning combined with our knowledge of the pharmacophore yielded 15 PKC ligand candidates, but we did not evaluate their PKC binding affinities fully. In this paper, PKC binding affinities of four candidates were examined to assess their potential as PKC ligands and to validate machine learning-assisted screening. Although compound 3' did not bind to PKC C1 domains, 1a, 2', and 4a exhibited moderate PKC binding affinities, suggesting that machine learning-assisted screening is advantageous in identifying new PKC ligand scaffolds.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"668-679"},"PeriodicalIF":1.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structure of l-2-keto-3-deoxyrhamnonate 4-dehydrogenase involved in the non-phosphorylating pathway of l-rhamnose metabolism by bacteria. 参与细菌 L-鼠李糖代谢非磷酸化途径的 L-2-keto-3-deoxyrhamnonate 4-dehydrogenase 晶体结构。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf015

Miyu Akagashi, Seiya Watanabe

{"title":"Crystal structure of l-2-keto-3-deoxyrhamnonate 4-dehydrogenase involved in the non-phosphorylating pathway of l-rhamnose metabolism by bacteria.","authors":"Miyu Akagashi, Seiya Watanabe","doi":"10.1093/bbb/zbaf015","DOIUrl":"10.1093/bbb/zbaf015","url":null,"abstract":"In the non-phosphorylative l-rhamnose and l-fucose pathways in bacteria, the C4-OH groups of the l-2-keto-3-deoxyrhamnonate (l-KDR) and l-2-keto-3-deoxyfuconate (l-KDF) intermediates are oxidized by different NAD+-dependent dehydrogenases, which belong to the same superfamily: l-KDRDH and l-KDFDH, respectively. To further elucidate their opposite stereospecificities, we herein investigated the crystal structures of l-KDRDH (from Herbaspirillum huttiense) in ligand-free and NAD+-bound forms. The interactions between the side chains of Asp39 and Gln18, and the 2'- and/or 3'-hydroxyl group(s) of NAD+ were consistent with strict specificity for NAD+. In a binding model for the substrate, Asn151 and Arg247 interacted with the C1 carboxyl and/or C5 hydroxyl group(s) of l-KDR with the acrylic α-keto form, which differed from l-KDFDH, which recognizes l-KDF with the cyclic hemiketal. A comparison of gene clusters on the bacterial genome and biochemical characterization suggested that l-KDRDH functions as a novel 4-hydroxy-2-oxopentanoate dehydrogenase in the degradation of aromatic compounds.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"733-742"},"PeriodicalIF":1.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oregano leaf odor regulates sodium chloride consumption in mice. 牛至叶气味调节小鼠氯化钠摄入量。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf014

Kazumi Osada, Nanako Akiyama, Akira Hosono, Motoko Ohata, Issei Yokoyama, Sadaharu Miyazono, Michio Komai

引用次数: 0

Synthesis and characterization of thermally stable and water-soluble pantetheine trisulfide and its composites. 热稳定水溶性三硫化泛硫氨酸及其复合材料的合成与表征。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf010

Shoichiro Tomonaga, Hiroaki Ishimaru, Shizuki Misada, Takahiro Isobe, Etsuo Ohshima, Shinji Kitagaki

引用次数: 0

Synthesis of (S)-4-methylgeranyl esters, the pheromone components of the ponerine ant, Holcoponera striatula, and their (R)-isomers. (S)-4-甲基香叶醚酯及其(R)-异构体的合成。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf021

Takuya Tashiro, Hiroyuki Watanabe

引用次数: 0

Modification of intracellular metabolism by expression of a C-terminal variant of phosphoribulokinase from Synechocystis sp. PCC 6803. 通过表达 Synechocystis sp. PCC 6803 的磷酸纤维素激酶 C 端变体改变细胞内代谢。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf013

Hiroki Nishiguchi, Teppei Niide, Yoshihiro Toya, Hiroshi Shimizu

引用次数: 0

Malate inhibits light-induced stomatal opening through SLAC1- and G-proteins mediated pathway in grapevine and Arabidopsis. 苹果酸盐通过SLAC1-和g蛋白介导的途径抑制葡萄和拟南芥光诱导的气孔打开。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf011

Zhongyi Yang, Ruhai Gong, Yoshiharu Mimata, Shaosong Ye, Wei Ji, Wenxiu Ye

{"title":"Malate inhibits light-induced stomatal opening through SLAC1- and G-proteins mediated pathway in grapevine and Arabidopsis.","authors":"Zhongyi Yang, Ruhai Gong, Yoshiharu Mimata, Shaosong Ye, Wei Ji, Wenxiu Ye","doi":"10.1093/bbb/zbaf011","DOIUrl":"10.1093/bbb/zbaf011","url":null,"abstract":"A key tricarboxylic acid (TCA) cycle metabolite, malate, accumulates in leaves during dehydration and induces stomatal closure by recruiting cytosolic Ca2+, activating SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1), and promoting reactive oxygen species (ROS). However, the effects of malate on stomatal opening and its underlying molecular mechanisms remain poorly understood. Our study revealed that, among TCA cycle metabolites, malate specifically inhibited light-induced stomatal opening in both grapevine and Arabidopsis. We demonstrated that SLAC1 was required for malate's inhibitory effects. The inhibition by malate was disrupted by Ca2+ signaling inhibitors. Additionally, the malate signal was mediated by G-proteins, which regulate the production of second messengers. ROS production was abolished when G-proteins were inhibited. These findings show that malate efficiently maintains stomatal closure by not only inducing stomatal closure but also inhibiting stomatal opening. The inhibition of stomatal opening by malate is mediated through the activation of SLAC1 and the G-protein signaling cascade.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"693-703"},"PeriodicalIF":1.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0