一种透气性高、透明的塑料薄膜袋，方便观察无菌培养细胞。

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-23 DOI:10.1093/bbb/zbaf078

Yuki Naka, Motomu Akita

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引用次数: 0

摘要

介绍了一种利用TPX膜袋对外植体培养物进行连续显微观察的培养方法。我们以藓类藓苔（Physcomitrium patens）为模型。绿色荧光蛋白在苔藓配子体中的表达是可以观察到的，并且在膜袋培养体系中原生质体可以再生成原丝体。我们证明，薄膜袋系统可以观察和分析植物与真菌的相互作用。

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Utility of a plastic film bag with high gas permeability and transparency for convenient observation of aseptic cultured cells.

We introduce a culture method using TPX film bags for continuous microscopic observation of explant cultures. We used the moss Physcomitrium patens as a model system. Expression of GFP in moss gametophores is observable and protoplast regeneration into protonemata is achievable in the film-bag culture system. We demonstrate that the film-bag system allows observation and analysis of plant-fungus interaction.

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).