Bioscience, Biotechnology, and Biochemistry最新文献

Characterization of N-malonylurea hydrolase in the pyrimidine oxidative degradation pathway of Rhodococcus erythropolis JCM 3132. 红红红球菌JCM 3132嘧啶氧化降解途径中n -丙二酰脲水解酶的表征

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-16 DOI: 10.1093/bbb/zbaf083

Kengo Deguchi, Nobuyuki Horinouchi, Michiki Takeuchi, Chee-Leong Soong, Sakayu Shimizu, Jun Ogawa

引用次数: 0

Conferring transfer capability by cloning transfer-related genes from the conjugative linear plasmid SAP1. 从共轭线性质粒SAP1克隆转移相关基因以获得转移能力。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-14 DOI: 10.1093/bbb/zbaf090

Yuta Fukada, Naoya Maruyama, Shota Endou, Haruo Ikeda, Masakazu Kataoka

引用次数: 0

Efficient production of D-sedoheptulose using transketolase from Thermus thermophilus HB8 and epimerization reaction using D-tagatose 3-epimerase from Pseudomonas cichorii ST24. 利用嗜热热菌HB8的转酮醇酶和池氏假单胞菌ST24的d -塔格糖3-外聚酶进行高效产d -糖庚糖的反应。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-14 DOI: 10.1093/bbb/zbaf088

Kana Watanuki, Susumu Mochizuki, Yusuke Hanaki, Hiromi Yoshida, Shigehiro Kamitori, Ken Izumori, Akihide Yoshihara

{"title":"Efficient production of D-sedoheptulose using transketolase from Thermus thermophilus HB8 and epimerization reaction using D-tagatose 3-epimerase from Pseudomonas cichorii ST24.","authors":"Kana Watanuki, Susumu Mochizuki, Yusuke Hanaki, Hiromi Yoshida, Shigehiro Kamitori, Ken Izumori, Akihide Yoshihara","doi":"10.1093/bbb/zbaf088","DOIUrl":"https://doi.org/10.1093/bbb/zbaf088","url":null,"abstract":"Heptose is a rare monosaccharide with seven carbon atoms. In this study, we demonstrated that two heptoses can be produced cost-effectively and efficiently using transketolase from Thermus thermophilus HB8 (TtTK). First, alternative substrates to a conventional donor substrate, lithium hydroxypyruvate, were investigated, suggesting that 5-keto-D-fructose (5-KF) was the best donor substrate among the 14 donor substrates evaluated. Using 5-KF and aldopentoses as acceptors, we confirmed that four heptoses could be produced, and that the optimal reaction temperature and donor and acceptor substrate concentration are 50°C and 500 mM, respectively. Furthermore, the D-sedoheptulose produced by the partially purified TtTK was converted to D-allopeptulose through the epimerization by D-tagatose 3-epimerase (D-TE) from Pseudomonas. cichorii ST24. The equilibrium ratio of the epimerization reaction between D-sedoheptulose and D-alloheptulose was 45:55. This is the first report that D-TEcould catalyze epimerization reaction between D-sedoheptulose and D-alloheptulose.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of GDE1 decreases glycerophosphoinositol in intestinal epithelial cells in vivo. 体内过表达GDE1可降低肠上皮细胞中的甘油磷酸肌醇。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-13 DOI: 10.1093/bbb/zbaf087

Siyi Chen, Rahmawati Aisyah, Ayako Honda, Takahiro Kudo, Yusuke Sotomaru, Satoshi Ishizuka, Thanutchaporn Kumrungsee, Toshihiko Iwanaga, Noriyuki Yanaka

{"title":"Overexpression of GDE1 decreases glycerophosphoinositol in intestinal epithelial cells in vivo.","authors":"Siyi Chen, Rahmawati Aisyah, Ayako Honda, Takahiro Kudo, Yusuke Sotomaru, Satoshi Ishizuka, Thanutchaporn Kumrungsee, Toshihiko Iwanaga, Noriyuki Yanaka","doi":"10.1093/bbb/zbaf087","DOIUrl":"https://doi.org/10.1093/bbb/zbaf087","url":null,"abstract":"Glycerophosphoinositol (GPI), a product of phospholipases acting on membrane phosphoinositides, is increasingly being recognized as an important bioactive molecule. However, its cellular functions in vivo remain unexplored. Recently, several mammalian glycerophosphodiesterases (GDEs) have shown to be involved in the catabolism of GPI, possibly regulating its intracellular levels. Given the growing interest in biological significance of GPI in intestinal epithelial cells, this study focused on screening a GDE involved in intracellular GPI metabolism. Our results showed that GDE1 mRNA was highly expressed in intestinal epithelial cells and that its overexpression decreased intracellular GPI in cultured cells. We then created transgenic mice with GDE1 overexpression specifically in intestinal epithelial cells. These mice showed that GDE1 protein is localized in epithelial cells and that GPI levels are downregulated in the colon epithelial layers. This study provides a novel animal model for exploring GPI functions in vivo.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Impact of Polysaccharide from Chinese Yam (Dioscorea opposita Thunb) on DSS-Induced Ulcerative Colitis in Mice. 山药多糖对dss致小鼠溃疡性结肠炎的治疗作用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-11 DOI: 10.1093/bbb/zbaf086

Jianing Tan, Ziliang Zhuo, Ling Zhang, Xuemin Jin, Xiuyu Wang, Yanshuang Zhang, Yajun Zhou

{"title":"Therapeutic Impact of Polysaccharide from Chinese Yam (Dioscorea opposita Thunb) on DSS-Induced Ulcerative Colitis in Mice.","authors":"Jianing Tan, Ziliang Zhuo, Ling Zhang, Xuemin Jin, Xiuyu Wang, Yanshuang Zhang, Yajun Zhou","doi":"10.1093/bbb/zbaf086","DOIUrl":"https://doi.org/10.1093/bbb/zbaf086","url":null,"abstract":"While yam polysaccharide (YP) demonstrates diverse biological activities, its anti-inflammatory mechanisms remain insufficiently characterized. This study systematically investigates the therapeutic efficacy of YP in ulcerative colitis (UC) through integrated in vitro and in vivo approaches. YP was purified from Dioscorea opposita via ethanol precipitation, followed by cytotoxicity profiling. In LPS-stimulated intestinal epithelial cells (IEC-6), YP significantly attenuated pro-inflammatory responses. For in vivo validation, a murine colitis model was established through dextran sulfate sodium (DSS) administration in drinking water. Systematic macroscopic and histopathological assessments revealed that YP treatment reduced colon shortening and mitigated crypt architectural distortion. Further analyses combining H&E staining, immunofluorescence, and western blotting demonstrated that YP exerts anti-inflammatory effects through upregulating interleukin-10 (IL-10) expression. These findings position YP as a multifaceted modulator of intestinal inflammation, suggesting its potential as a novel therapeutic agent for UC management.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of isomaltose transporters in Aspergilli demonstrated through nuclear translocation assays of the transcription factor AmyR. 通过转录因子AmyR的核易位测定，证实了曲霉中存在异麦芽糖转运体。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-09 DOI: 10.1093/bbb/zbaf085

Shoki Fujita, Da Min Jeong, Takanori Ichikawa, Takahiro Shintani, Katsuya Gomi

{"title":"Presence of isomaltose transporters in Aspergilli demonstrated through nuclear translocation assays of the transcription factor AmyR.","authors":"Shoki Fujita, Da Min Jeong, Takanori Ichikawa, Takahiro Shintani, Katsuya Gomi","doi":"10.1093/bbb/zbaf085","DOIUrl":"https://doi.org/10.1093/bbb/zbaf085","url":null,"abstract":"Maltose is readily incorporated into Aspergillus oryzae cells. In contrast, isomaltose is unlikely to be incorporated into both A. oryzae and Aspergillus nidulans, even though extremely low concentrations of isomaltose can induce the nuclear translocation of AmyR. This suggests the presence of sensor-like proteins or isomaltose transporters in both Aspergilli. In this study, we showed that isomaltose can be incorporated via sugar/H+ symporters using AmyR nuclear translocation assays. Assuming that sugar transporters are proton-coupled symporters, we examined the effect of the protonophore carbonyl cyanide m-chlorophenyl hydrazone on isomaltose-induced AmyR nuclear translocation. Consequently, AmyR nuclear translocation was impaired by the addition of the protonophore in A. oryzae. This was confirmed under high pH conditions where AmyR nuclear translocation was abrogated in both Aspergilli. These results suggest that instead of sensors, isomaltose is most likely incorporated by sugar/H+ symporters with low isomaltose transport activity, triggering isomaltose-induced AmyR nuclear translocation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic resource and hormonal profiling of the smut fungus, Ustilago esculenta, coexisting with Zizania latifolia and forming Makomotake. 黑穗病菌黑穗病菌（Ustilago esculenta）与紫穗病菌（Zizania latifolia）共存并形成Makomotake的转录组资源和激素分析。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-06 DOI: 10.1093/bbb/zbaf084

Yuki Tanaka, Akiko Ono, Daichi Aoki, Rina Matsuda, Yuina Kitsukawa, Jae-Hoon Choi, Xiaonan Xie, M Catherine Aime, Hirokazu Kawagishi, Tomohiro Suzuki

{"title":"Transcriptomic resource and hormonal profiling of the smut fungus, Ustilago esculenta, coexisting with Zizania latifolia and forming Makomotake.","authors":"Yuki Tanaka, Akiko Ono, Daichi Aoki, Rina Matsuda, Yuina Kitsukawa, Jae-Hoon Choi, Xiaonan Xie, M Catherine Aime, Hirokazu Kawagishi, Tomohiro Suzuki","doi":"10.1093/bbb/zbaf084","DOIUrl":"https://doi.org/10.1093/bbb/zbaf084","url":null,"abstract":"Ustilago esculenta is a smut fungus that parasitizes Zizania latifolia, forming edible fungal galls known as Makomotake. In this study, we quantified indole-3-acetic acid (IAA) produced in liquid-cultured U. esculenta and in fungal galls at different growth stages. IAA was detected in both samples, and its content showed a tendency to increase during the mature growth stage in October. Furthermore, RNA-seq analysis was performed to investigate gene expression changes in U. esculenta during different developmental stages. The gene involved in spore maturation was upregulated in October, consistent with fungal maturation. Moreover, numerous genes with unknown functions were significantly upregulated, highlighting potential targets for further investigation. These findings provide insights into the molecular mechanisms underlying fungal gall development and the symbiotic relationship between U. esculenta and Z. latifolia, providing a foundation for future research on fungal-plant interactions.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A unique tetrasaccharide-containing anchor glycolipid of lipoteichoic acid is commonly found in Lactobacillus gasseri and Lactobacillus paragasseri. 一种独特的含有脂磷胆酸的四糖锚定糖脂常见于干酪乳杆菌和副干酪乳杆菌中。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-02 DOI: 10.1093/bbb/zbaf081

Tsukasa Shiraishi, Ryosuke Kutomi, Yamaha Sato, Akihito Endo, Satoru Fukiya, Satoshi Takahashi, Atsushi Yokota, Shin-Ichi Yokota

{"title":"A unique tetrasaccharide-containing anchor glycolipid of lipoteichoic acid is commonly found in Lactobacillus gasseri and Lactobacillus paragasseri.","authors":"Tsukasa Shiraishi, Ryosuke Kutomi, Yamaha Sato, Akihito Endo, Satoru Fukiya, Satoshi Takahashi, Atsushi Yokota, Shin-Ichi Yokota","doi":"10.1093/bbb/zbaf081","DOIUrl":"https://doi.org/10.1093/bbb/zbaf081","url":null,"abstract":"Lipoteichoic acid (LTA) is a cell surface molecule with physiologically important roles in Gram-positive bacteria. Previously, we identified a novel tetrasaccharide-containing anchor glycolipid in the Lactobacillus gasseri JCM 1131T LTA. However, it is unclear whether this tetrasaccharide-containing anchor glycolipid structure is characteristic of JCM 1131T alone or is commonly found in other L. gasseri-related isolates. Lactobacillus paragasseri has been proposed as a new species, separated from the L. gasseri. In the present study, we characterized chemical structures of LTA from two strains of L. gasseri and four strains of L. paragasseri. All six strains shared a polymer comprising repeating units of glycerophosphate with partial D-alanine substitutions, as well as L. gasseri JCM 1131T. Anchor glycolipid structure suggested that all strains shared a tetrasaccharide-containing glyceroglycolipid with two and three acyl groups. Focusing on LTA from L. gasseri and L. paragasseri species, a tetrasaccharide-containing anchor glycolipid is a specific feature.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brominated bitter compounds from the skin tubercle gland of the stonefish Synanceia verrucosa. 从石鱼的皮肤结节腺中提取的溴化苦味化合物。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-02 DOI: 10.1093/bbb/zbaf082

Ryoya Kawabe, Sota Hoshi, Toru Hifumi, Akihiko Yamamoto, Hajime Uchida, Masayuki Satake, Hiroshi Nagai

引用次数: 0

Microbial biotransformation of proteins into amino acids in unpolished Thai and polished Japanese rice varieties cultivated with distinct industrial strains of koji mold. 用不同工业菌种栽培的泰国和日本未抛光水稻品种中蛋白质向氨基酸的微生物转化。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-27 DOI: 10.1093/bbb/zbaf079

Jirayu Jitpakdee, Kazunari Ito, Yuka Tanino, Hayato Takeuchi, Hideyuki Yamashita, Takuro Nakagawa, Teruhiko Nitoda, Hiroshi Kanzaki

{"title":"Microbial biotransformation of proteins into amino acids in unpolished Thai and polished Japanese rice varieties cultivated with distinct industrial strains of koji mold.","authors":"Jirayu Jitpakdee, Kazunari Ito, Yuka Tanino, Hayato Takeuchi, Hideyuki Yamashita, Takuro Nakagawa, Teruhiko Nitoda, Hiroshi Kanzaki","doi":"10.1093/bbb/zbaf079","DOIUrl":"https://doi.org/10.1093/bbb/zbaf079","url":null,"abstract":"We previously reported the cultivation of industrial koji mold strains to produce unpolished Thai-colored rice kojis. These kojis, along with those made from unpolished Thai white rice and polished Japanese white rice, showed increased polyphenol content after cultivation, with the highest levels observed in unpolished Thai-colored rice kojis. In this study, an increase in both proteinogenic and non-proteinogenic amino acid contents, particularly GABA content, was observed in both unpolished Thai and polished Japanese rice kojis, suggesting the ability of koji mold in the biotransformation of proteins. This increase was almost comparable even when using different rice varieties; in contrast, it varied depending on the koji mold strain used. The observed increase in both polyphenol and functional amino acid contents, especially GABA content, highlights the potential of unpolished Thai and polished Japanese rice kojis, particularly unpolished Thai-colored rice koji, as multifunctional materials, benefiting from polyphenol and amino acid functionalities.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0