Bioscience, Biotechnology, and Biochemistry最新文献

A macrophage-conditioned medium model recapitulates inflammation-associated suppression of UCP-1 expression in KK-Ay mice. 巨噬细胞条件培养基模型重现了KK-Ay小鼠炎症相关的UCP-1表达抑制。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-01 DOI: 10.1093/bbb/zbag133

Hisako Takahashi, Hiromu Morimoto, Hirofumi Inoue, Kentaro Kaneko, Tsuyoshi Goto, Teruo Kawada, Mariko Uehara, Nobuyuki Takahashi

引用次数: 0

Development of a defined three-strain nitrifying microbial community for ammonification and nitrification under organic nitrogen conditions. 在有机氮条件下进行氨化和硝化的确定的三株硝化微生物群落的发展。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-29 DOI: 10.1093/bbb/zbag131

Htun Inzali, Sakuntala Saijai, Wakako Okada, Jun Ogawa, Akinori Ando

引用次数: 0

Syntheses of Nostodione A and Half Scytonemins. 迷迭酮A和半丝胞酮胺的合成。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-26 DOI: 10.1093/bbb/zbag130

Yutaro Udagawa, Seijiro Hosokawa

引用次数: 0

Cloning and characterization of a novel oleic acid hydratase from the filamentous fungus Fusarium sp. D2. 丝状真菌镰刀菌（Fusarium sp. D2）油酸水合酶的克隆与鉴定。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-24 DOI: 10.1093/bbb/zbag091

Takaiku Sakamoto, Naomi Murakawa, Yume Shibata, Shigenobu Kishino, Jun Ogawa, Eiji Sakuradani

引用次数: 0

Concomitant short-term ingestion of fucoidan improves the initial inflammation and immune dysfunction caused by a high-fat diet. 同时短期摄入岩藻糖聚糖可改善由高脂肪饮食引起的初始炎症和免疫功能障碍。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-24 DOI: 10.1093/bbb/zbag081

Sunao Abe, Hyo Hamamoto, Hiro Kobayashi, Yoshikazu Saito, Tomoko Ishijima, Shinji Okada, Keiko Abe, Hiroko Hoshi

{"title":"Concomitant short-term ingestion of fucoidan improves the initial inflammation and immune dysfunction caused by a high-fat diet.","authors":"Sunao Abe, Hyo Hamamoto, Hiro Kobayashi, Yoshikazu Saito, Tomoko Ishijima, Shinji Okada, Keiko Abe, Hiroko Hoshi","doi":"10.1093/bbb/zbag081","DOIUrl":"10.1093/bbb/zbag081","url":null,"abstract":"We examined the effects of fucoidan, a sulfated polysaccharide, administered concurrently with the onset of a high-fat diet (HFD) for a short period in C57BL/6 N mice to evaluate its immunological effects under obesity-inducing conditions. Numerous genes that were downregulated in the HFD group relative to the control-diet group were restored in the HFD + fucoidan group; these genes were mainly associated with inflammatory responses and cellular functions, including migration and viability, as identified by DNA microarray analysis. Upstream regulator analysis in ingenuity pathway analysis identified numerous cytokines and inflammatory mediators. Quantitative PCR confirmed that the expression of selected inflammatory cytokine genes-particularly Ifn-γ and Il-5-was decreased in the HFD group but restored in the HFD + fucoidan group. Our study suggests that fucoidan administered concurrently with an HFD attenuates HFD-induced downregulation of inflammatory and immune-related functions, including T- and B-cell functions, and may improve HFD-induced immune dysfunction and imbalance.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1228-1236"},"PeriodicalIF":1.5,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insig2 3'UTR shortening by alternative polyadenylation escapes from miR-96-5p-mediated regulation and inhibits adipogenesis. 通过选择性聚腺苷化缩短Insig2 3'UTR，可以逃避mir -96-5p介导的调节，并抑制脂肪形成。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-24 DOI: 10.1093/bbb/zbag095

Shizhong Ren, Xuyong Qin, Lichun Zhang, Chengping Li, Yuanyuan Yu, Wenhao Zhou, Meiman Zhao, Guoli Zhou

{"title":"Insig2 3'UTR shortening by alternative polyadenylation escapes from miR-96-5p-mediated regulation and inhibits adipogenesis.","authors":"Shizhong Ren, Xuyong Qin, Lichun Zhang, Chengping Li, Yuanyuan Yu, Wenhao Zhou, Meiman Zhao, Guoli Zhou","doi":"10.1093/bbb/zbag095","DOIUrl":"10.1093/bbb/zbag095","url":null,"abstract":"Alternative polyadenylation (APA) is a key post-transcriptional regulatory mechanism in eukaryotes, but its role in adipogenesis remains poorly understood. Here, we investigated how insulin-induced gene 2 (Insig2) APA regulates adipogenesis. Functional assays showed that Insig2 knockdown promoted 3T3-L1 preadipocyte differentiation, whereas Insig2 overexpression inhibited it. During adipogenesis, Insig2 generated two APA isoforms with distinct 3'UTR lengths, and the short 3'UTR isoform was more abundant than the long isoform. Mechanistically, miR-96-5p specifically targeted the unique extension region of the long Insig2 3'UTR isoform to suppress its expression, thereby alleviating its inhibitory effect on adipogenesis. In contrast, the short Insig2 isoform lacked the miR-96-5p binding site and escaped miR-96-5p-mediated repression, thus exerting a stronger inhibitory effect on adipogenesis. Collectively, these findings reveal an isoform-specific Insig2 APA/miR-96-5p regulatory axis that dynamically controls adipogenesis at the post-transcriptional level. Our results revealed a new regulatory mechanism of Insig2 APA in adipogenesis.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1171-1181"},"PeriodicalIF":1.5,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hot-water extract of defatted Perilla frutescens seed residue suppresses osteoclast differentiation and improves bone-related parameters in ovariectomized mice. 脱脂紫苏籽渣热水提取物抑制去卵巢小鼠破骨细胞分化，改善骨相关参数。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-24 DOI: 10.1093/bbb/zbag082

Hiroyuki Asano, Sogo Nishimoto

引用次数: 0

Altered flagellin-TLR5 signaling pathway in the interaction between the host and laboratory Escherichia coli strains engineered for enhanced intestinal colonization in mice. 鞭毛蛋白- tlr5信号通路在宿主与实验室大肠杆菌菌株相互作用中的改变，以增强小鼠肠道定植。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-24 DOI: 10.1093/bbb/zbag079

Tsuyoshi Tachibana, Atsushi Minami, Ryutaro Fukui, Ryota Tochinai, Kohei Mochizuki, Wataru Fujii, Tetsuhiro Ogawa, Shigeru Kakuta

{"title":"Altered flagellin-TLR5 signaling pathway in the interaction between the host and laboratory Escherichia coli strains engineered for enhanced intestinal colonization in mice.","authors":"Tsuyoshi Tachibana, Atsushi Minami, Ryutaro Fukui, Ryota Tochinai, Kohei Mochizuki, Wataru Fujii, Tetsuhiro Ogawa, Shigeru Kakuta","doi":"10.1093/bbb/zbag079","DOIUrl":"10.1093/bbb/zbag079","url":null,"abstract":"The intestinal microbiota is essential for maintaining intestinal homeostasis by regulating mucosal immune responses. Identifying bacterial traits that support stable host interactions is therefore crucial for developing next-generation mucosal probiotics. RNase T2 is a conserved ribonuclease with species-specific functions. In Escherichia coli, RNase T2 (RNase I) suppresses biofilm formation, a key process for intestinal colonization. We previously showed that an RNase I-deficient E. coli (Δrna) strain stably colonizes the mouse intestine. Here, we analyzed the immunogenic properties of the Δrna strain. Heat-killed Δrna induced an early IL-1β and TNF-α-skewed cytokine response in RAW264.7 macrophages, with reduced IL-6 expression compared to wild type. In mice, Δrna colonization caused no intestinal tissue injury but enhanced IL-6 and IL-22 expression in the cecum and colon. Selected TLR-specific reporter assays identified TLR5-dependent signaling driven by increased FliC expression. These findings show that RNase I deficiency modulates immune activation while maintaining non-pathogenic mucosal interactions.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1265-1272"},"PeriodicalIF":1.5,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148210095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic profiling of LPS-induced systemic inflammation in mice: tryptophan as a biomarker for nutritional and inflammatory status. lps诱导小鼠全身性炎症的代谢组学分析：色氨酸作为营养和炎症状态的生物标志物。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-24 DOI: 10.1093/bbb/zbag078

Rio Kurihara, Yumi Takayama, Riyo Hidaka, Kanae Masuda, Hikono Sakata, Yukina Yumen, Tomomi Komura, Daisuke Saigusa, Masaru Yoshida

{"title":"Metabolomic profiling of LPS-induced systemic inflammation in mice: tryptophan as a biomarker for nutritional and inflammatory status.","authors":"Rio Kurihara, Yumi Takayama, Riyo Hidaka, Kanae Masuda, Hikono Sakata, Yukina Yumen, Tomomi Komura, Daisuke Saigusa, Masaru Yoshida","doi":"10.1093/bbb/zbag078","DOIUrl":"10.1093/bbb/zbag078","url":null,"abstract":"Lipopolysaccharide (LPS)-induced inflammation triggers metabolic reprogramming and nutritional decline. This study aimed to identify biomarkers for inflammatory and nutritional stress using gas chromatography-mass spectrometry (GC-MS/MS)-based metabolomic profiling. Male mice received LPS to induce systemic inflammation. Evaluations included liver histology (H&E, Gr-1), blood biochemistry, and metabolomic analysis of liver and plasma. LPS administration significantly increased hepatic neutrophil infiltration and liver enzymes, while decreasing nutritional markers (total protein, albumin, LDL-cholesterol). In the liver, LPS increased glycolytic and TCAcycle intermediates (e.g. 3-phosphoglycerate, citric acid) but decreased amino acids, including glutamine and tryptophan. Plasma analysis showed significant decreases in tryptophan, glucose, and succinic acid. Notably, tryptophan was significantly reduced in both compartments. Our findings demonstrate that tryptophan serves as a robust biomarker for monitoring the intersection of inflammatory response and nutritional status, reflecting synchronized metabolic shifts in the liver and plasma.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1237-1244"},"PeriodicalIF":1.5,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurite outgrowth-promoting furanocoumarins isolated from sudachi juice in PC12 cells. 从水achi果汁中分离的促进神经突生长的呋喃香豆素在PC12细胞中的作用。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-08-24 DOI: 10.1093/bbb/zbag092

Saki Morimoto, Takeru Koga, Saho Miyazaki, Akihiro Tai

{"title":"Neurite outgrowth-promoting furanocoumarins isolated from sudachi juice in PC12 cells.","authors":"Saki Morimoto, Takeru Koga, Saho Miyazaki, Akihiro Tai","doi":"10.1093/bbb/zbag092","DOIUrl":"10.1093/bbb/zbag092","url":null,"abstract":"Sudachi (Citrus sudachi, a citrus fruit of the Rutaceae family) juice was found to promote neurite outgrowth in PC12 cells. Activity-guided purification of the juice led to the isolation of two furanocoumarins, byakangelicin and isopimpinellin, and a flavone, demethoxysudachitin, identifying them as neurite-outgrowth promoters for the first time. Byakangelicin and isopimpinellin showed markedly stronger neurite outgrowth-promoting activity than demethoxysudachitin, prompting a comparative evaluation of five furanocoumarins, including both isolated and related reference compounds. The results showed that the basic furanocoumarin scaffold alone is inactive, whereas substitution at either the C5 or C8 position of a furanocoumarin confers prominent activity, with dual substitution further improving the activity. Among the tested compounds, byakangelicin and isopimpinellin exhibited the most potent neurite outgrowth-promoting activity. These findings identify sudachi-derived furanocoumarins as previously unrecognized neurite-outgrowth promoters and suggest their potential as neurotrophic agents for supporting cognitive function.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1147-1153"},"PeriodicalIF":1.5,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148307005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0