Bioscience, Biotechnology, and Biochemistry最新文献

Comprehensive analysis of the lipid composition in Saccharomyces cerevisiae, Saccharomyces eubayanus, and Saccharomyces pastorianus grown at different temperatures by untargeted lipidomics. 利用非靶向脂质组学对不同温度下培养的酿酒酵母、真巴酵母和pastorianus酵母的脂质组成进行综合分析。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-08 DOI: 10.1093/bbb/zbag067

Tomoko Takahashi, Ryo Iwama, Nobuyuki Okahashi, Fumio Matsuda, Ryouichi Fukuda, Ryo Nakasato, Yoichi Noda

{"title":"Comprehensive analysis of the lipid composition in Saccharomyces cerevisiae, Saccharomyces eubayanus, and Saccharomyces pastorianus grown at different temperatures by untargeted lipidomics.","authors":"Tomoko Takahashi, Ryo Iwama, Nobuyuki Okahashi, Fumio Matsuda, Ryouichi Fukuda, Ryo Nakasato, Yoichi Noda","doi":"10.1093/bbb/zbag067","DOIUrl":"https://doi.org/10.1093/bbb/zbag067","url":null,"abstract":"The brewer's yeast Saccharomyces pastorianus originated as a hybrid between the mesophilic yeast Saccharomyces cerevisiae and cryotolerant yeast Saccharomyces eubayanus. In this study, we investigated temperature-dependent changes in lipid composition in three yeast species using untargeted lipidomics. We found that both S. cerevisiae and S. pastorianus accumulated storage lipids, particularly triacylglycerols (TG), as the temperature decreased, whereas TG levels in S. eubayanus showed slight temperature-dependent changes. Mitochondria-associated lipids, such as cardiolipin (CL) and its precursor phosphatidylglycerol, also exhibited species-specific patterns. In particular, S. cerevisiae and S. eubayanus differed in their regulatory modes of fatty acid desaturation in CL. The acyl chain composition of CL in S. pastorianus was biased toward the characteristics of S. eubayanus. Our findings show that S. pastorianus integrates parental lipid regulatory systems in a lipid class-specific manner, combining S. cerevisiae-like regulation of storage lipids with S. eubayanus-like control of mitochondrial membrane lipids.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of a Salt- and Oxygen-Tolerant Bifidobacterium from Kishu-Narezushi. 一株Kishu-Narezushi耐盐耐氧双歧杆菌的分离与鉴定。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-30 DOI: 10.1093/bbb/zbag062

Takato Hirata, Koki Obai, Miki Kubo, Yuichiro Ikagawa, Hitoshi Iwahashi

{"title":"Isolation and Characterization of a Salt- and Oxygen-Tolerant Bifidobacterium from Kishu-Narezushi.","authors":"Takato Hirata, Koki Obai, Miki Kubo, Yuichiro Ikagawa, Hitoshi Iwahashi","doi":"10.1093/bbb/zbag062","DOIUrl":"https://doi.org/10.1093/bbb/zbag062","url":null,"abstract":"Bifidobacterium psychraerophilum was isolated from Kishu-Narezushi, marking the first discovery of the Bifidobacterium species originating from a spontaneously fermented traditional Japanese food. Kishu-Narezushi is a traditional Japanese spontaneously fermented food composed of fish, rice, and salt. Typically, Bifidobacterium species are isolated from milk-related fermented foods or human intestines. The isolated strain was identified as B. psychraerophilum and named the Yasuke strain. B. psychraerophilum are closely related with Bifidobacterium aquikeferi and Bifidobacterium crudilactis. The Yasuke strain and Bifidobacterium psychraerophilum DSM 22366 exhibited greater resistant to salt and oxygen compared to other Bifidobacterium species. Draft genome analysis of the Yasuke strain and four other B. psychraerophilum strains indicates the presence of stress tolerance-related genes. Superoxide dismutase [Mn] and glutathione peroxidase genes are observed in this species. Genes involved in phage interactions, proline metabolism, and sugar and amino acid transport were uniquely identified in the Yasuke strain.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147762623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Flux Control Enhances Intracellular Biosynthesis of Fumagillin Derivatives in Human Cells. 代谢通量控制增强人细胞内富马青霉素衍生物的生物合成。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-29 DOI: 10.1093/bbb/zbag061

Shinya Matsuda

引用次数: 0

This review was written in response to the author's receipt of the JSBBA Award in 2024. 这篇评论是为了回应作者在2024年获得JSBBA奖而写的。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-25 DOI: 10.1093/bbb/zbag060

Koji Uchida

引用次数: 0

Mitochondrial NADP+-isocitrate dehydrogenase Idp1 involves CoQ biosynthesis in parallel with NAD kinase Pos5. 线粒体NADP+-异柠檬酸脱氢酶Idp1与NAD激酶Pos5平行参与CoQ的生物合成。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-23 DOI: 10.1093/bbb/zbag059

Shogo Nishihara, Yasuhiro Matsuo, Makoto Kawamukai

引用次数: 0

De novo biosynthesis of phenylpyruvic acid by engineered Escherichia coli. 工程大肠杆菌新合成苯丙酮酸的研究。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag050

Xiaobing Yang, Chengbin Wang, Jingxin Yin, Jinli Wang, Dongxu Zhao, Jing Zhang, Haijun Gao

引用次数: 0

High-resolution profiling for the composition of various peptones by multimodal targeting analysis. 通过多模态靶向分析对各种蛋白胨的组成进行高分辨率分析。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag023

Takuto Nakajima, Kazuki Watanabe, Tomoko Kagenishi, Masaaki Konishi

{"title":"High-resolution profiling for the composition of various peptones by multimodal targeting analysis.","authors":"Takuto Nakajima, Kazuki Watanabe, Tomoko Kagenishi, Masaaki Konishi","doi":"10.1093/bbb/zbag023","DOIUrl":"10.1093/bbb/zbag023","url":null,"abstract":"Peptone is a promising raw material for culturing microorganisms and mammalian cells, but its composition remains unclear. Here, 36 different peptones were comprehensively quantified using five approaches: gas chromatography/tandem mass spectrometry, liquid chromatography/mass spectrometry, ion chromatography, postcolumn detection-high-performance liquid chromatography, and inductively coupled plasma mass spectrometry (named multimodal targeting analysis). Seventy-eight chemical compounds/ions were detected among 121 targets, including amino acids, nucleic acids, organic acids, sugars, vitamins, and minerals. The sum of the quantitated component weights exceeded 65% of the total weight in all cases (mean 88%). Principal component and cluster dendrogram analyses revealed clear distinctions between not only peptone brands but also production lots. Plant peptones exhibited greater variety than casein and meat peptones. Partial least squares analysis identified components specific to particular manufacturing processes and peptone sources. Acid-digested peptones contained more free amino acids, including Ala, Cys, Gly, Thr, Ser, Asp, Glu, and Pro, than enzyme-digested types.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"702-710"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An acute increase in systemic bile acid levels in rats treated with tunicamycin. tunicamycin治疗大鼠体内胆汁酸水平的急性升高。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag031

Fumika Yokoyama, Riho Yamada, Hiromichi Yamazaki, Honoka Maeda, Satoshi Ishizuka

引用次数: 0

Comparative functional evaluation of the atypically modified GlcHMS326 and porphyra-334, Two structurally distinct mycosporine-like amino acids. 非典型修饰GlcHMS326和Porphyra-334两种结构不同的真菌孢素样氨基酸的比较功能评价

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag011

Taiki Aono, Erika Katayama, Tomoki Tsuboi, Sasiprapa Samsri, Rungaroon Waditee-Sirisattha, Hakuto Kageyama

{"title":"Comparative functional evaluation of the atypically modified GlcHMS326 and porphyra-334, Two structurally distinct mycosporine-like amino acids.","authors":"Taiki Aono, Erika Katayama, Tomoki Tsuboi, Sasiprapa Samsri, Rungaroon Waditee-Sirisattha, Hakuto Kageyama","doi":"10.1093/bbb/zbag011","DOIUrl":"10.1093/bbb/zbag011","url":null,"abstract":"Mycosporine-like amino acids (MAAs) are natural compounds widely studied for their photoprotective and antioxidant properties. Typical MAAs consist of one or two amino acids attached to a cyclohexenone or cyclohexenimine ring, whereas atypical MAAs possess unique chemical modifications such as glycosylation and methylation. Recently, we identified an atypical MAA, GlcHMS326, from the cyanobacterium Gloeocapsa sp. BRSZ, characterized by glycosylation, methylation, and hydroxylation. In this study, we compared the chemical stability and biological activities of GlcHMS326 with those of a typical di-substituted MAA, porphyra-334. GlcHMS326 was less stable under high-temperature conditions but showed a slightly higher residual rate than porphyra-334 under the present visible-light exposure conditions. Functionally, GlcHMS326 showed stronger antioxidant and collagenase inhibitory activities but weaker antiglycative activity. Neither compound inhibited hyaluronidase activity. Both MAAs inhibited angiotensin-converting enzyme, with porphyra-334 showing stronger inhibition. These results provide insights into how chemical modifications influence the functional properties of MAAs.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"650-658"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different sterol compositions of honey bee Apis mellifera L. indicate role-dependent sterol metabolism. 蜜蜂不同的甾醇成分表明了角色依赖性的甾醇代谢。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag017

Makoto Ito, Hiroki Kohno, Hitomi Seike, Takao K Suzuki, Shinji Nagata

{"title":"Different sterol compositions of honey bee Apis mellifera L. indicate role-dependent sterol metabolism.","authors":"Makoto Ito, Hiroki Kohno, Hitomi Seike, Takao K Suzuki, Shinji Nagata","doi":"10.1093/bbb/zbag017","DOIUrl":"10.1093/bbb/zbag017","url":null,"abstract":"Honey bee Apis mellifera L. adults exhibit a sterol composition dominated by phytosterols such as 24-methylene-cholesterol and β-sitosterol, while most insects possess cholesterol as a primary sterol. To examine whether sterol composition varies by social roles, we analyzed nurse bees and foragers using gas chromatography-mass spectrometry. The analysis showed the four phytosterols, with 24-methylene-cholesterol being predominant. Honey, a dietary source for honey bees, contained more campesterol than in the whole body, suggesting possible metabolic use. Few significant differences in sterol compositions among tissues indicated that sterol metabolism is not tissue specific. However, social roles influenced different sterol compositions; nurse bees had higher β-sitosterol and isofucosterol, while foragers had more 24-methylene-cholesterol. Additionally, nurse bees retained more 24-methylene-cholesterol in their midgut. These findings suggest that sterol metabolism is influenced by social roles, probably due to differences in diet, absorption, or retention, rather than tissue dependent metabolism.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"587-596"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0