Bioscience, Biotechnology, and Biochemistry最新文献_第10页

Inhibitory activity of amyloid-β aggregation by flavonoids and rosmarinic acid related compounds isolated from Zostera marina. 黄酮类化合物和迷迭香酸相关化合物抑制淀粉样蛋白-β聚集活性的研究

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf058

Mitsuhiro Sekiguchi, Mafumi Horiuchi, Ayuko Tanaka, Yuki Yamada, Yuta Tozawa, Yasuki Higashimura, Hideyuki Shigemori

引用次数: 0

Marine bacterium Cobetia sp. strain GM produces fucose-rich extracellular polymeric substance. 海洋细菌Cobetia sp.菌株GM产生富聚焦的胞外聚合物质。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf025

Joe Watanabe, Kenjiro Sugiyama, Katsuhiko Fujii

引用次数: 0

Biochemical and structural analysis of the mechanism for the catalysis and specificity of cellobiose 2-epimerase from Rhodothermus marinus. 海红藻纤维二糖2-外甲酰基酶的催化机制及特异性的生化和结构分析。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf042

Wataru Saburi, Hirohiko Muto-Fukiya, Nongluck Jaito, Koji Kato, Jian Yu, Min Yao, Haruhide Mori

{"title":"Biochemical and structural analysis of the mechanism for the catalysis and specificity of cellobiose 2-epimerase from Rhodothermus marinus.","authors":"Wataru Saburi, Hirohiko Muto-Fukiya, Nongluck Jaito, Koji Kato, Jian Yu, Min Yao, Haruhide Mori","doi":"10.1093/bbb/zbaf042","DOIUrl":"10.1093/bbb/zbaf042","url":null,"abstract":"Cellobiose 2-epimerase (CE) catalyzes C-2 epimerization of reducing end d-glucose/d-mannose residue of β-(1→4)-disaccharides, and also slightly catalyzes aldose-ketose conversion. In this study, we investigated the structure-function relationship of Rhodothermus marinus CE (RmCEs). In 2H2O, 2H replaced the 2-H of the reducing end sugar residue, suggesting a proton abstraction-addition mechanism via the cis-enediolate intermediate. The structure of the RmCE-mannobiitol complex showed that His259 was suitable for abstracting 2-H from d-mannose residue, whereas His390 was suitable for the d-glucose residue. H259A and H390A mutations abolished activity for Galβ1-4Man and Galβ1-4Glc formation from Galβ1-4Fru, respectively, and these mutants catalyzed both epimerization and isomerization to Galβ1-4Glc and Galβ1-4Man, respectively. Ala substitution of the residues interacting with the 2-O of the reducing end sugar residue significantly reduced the velocity for epimerization, but not for isomerization. Trp385, stacked onto the non-reducing-end sugar residues of disaccharides, was shown to be important for disaccharide specificity.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"973-984"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the bread containing resistant starch from wheat (Triticum aestivum) on the improvement in bowel movements: a randomized, double-blind, placebo-controlled, crossover study. 含有小麦抗性淀粉（Triticum aestivum）的面包对改善肠道运动的影响：一项随机、双盲、安慰剂对照的交叉研究。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf044

Yosuke Kikuchi, Yuto Otomo, Kazuki Toyota, Satoshi Noma, Shukuko Ebihara

{"title":"Effects of the bread containing resistant starch from wheat (Triticum aestivum) on the improvement in bowel movements: a randomized, double-blind, placebo-controlled, crossover study.","authors":"Yosuke Kikuchi, Yuto Otomo, Kazuki Toyota, Satoshi Noma, Shukuko Ebihara","doi":"10.1093/bbb/zbaf044","DOIUrl":"10.1093/bbb/zbaf044","url":null,"abstract":"This study aimed to evaluate the gut-regulating effects of wheat-derived resistant starch. A randomized, double-blind, placebo-controlled crossover study was conducted with 46 healthy Japanese adults. They were divided into 2 groups, consuming 3.26 g/day of wheat-derived resistant starch and a control food for 4 weeks each, separated by a 4-week washout period. There were no significant differences observed in the primary endpoint of defecation frequency. However, subgroup analysis showed significant improvements in bowel movements for participants who felt a strong sensation of incomplete evacuation. No gut-regulating effects were noted in other subgroups and secondary endpoints. The intake of 3.26 g/day of resistant starch was safe with no overall efficacy observed. Increased stool frequency was observed in participants with less frequent bowel movements and a strong feeling of incomplete evacuation, but no effects were confirmed in other subgroup analyses. No significant changes were observed at secondary endpoints (UMIN ID: UMIN000052543).","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1015-1023"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regio-specific synthesis of flavonoid glucuronides using plant UDP-glucuronosyltransferase expressed in yeast. 利用酵母中表达的植物udp -葡萄糖醛基转移酶合成黄酮类葡萄糖醛酸酯的区域特异性研究。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf049

Mst Julia Sultana, Takuto Kurakawa, Miyu Nishikawa, Shinichi Ikushiro

{"title":"Regio-specific synthesis of flavonoid glucuronides using plant UDP-glucuronosyltransferase expressed in yeast.","authors":"Mst Julia Sultana, Takuto Kurakawa, Miyu Nishikawa, Shinichi Ikushiro","doi":"10.1093/bbb/zbaf049","DOIUrl":"10.1093/bbb/zbaf049","url":null,"abstract":"Glucuronidation is a well-established biotransformation process that modifies the physiological and pharmacological properties of small molecules, making it a valuable tool for enhancing the chemical diversity of natural compounds in drug development. However, the chemical synthesis of glucuronides is often complex, time-consuming, and environmentally unsustainable. To overcome these challenges, plant uridine diphosphate (UDP)-glucuronosyltransferase (UGT)-mediated glucuronidation, using transformed yeast, offers a selective and efficient alternative for producing flavonoid glucuronides. This study aimed to conjugate quercetin with glucuronic acid by stably co-transforming Saccharomyces cerevisiae with plant UGTs (UGT78A11 and UGT88D7) and rat UDP-glucose-6-dehydrogenease. The UGT78A11 and UGT88D7 selectively conjugated quercetin at specific positions, producing quercetin-3-O-glucuronide and quercetin-7-O-glucuronide, respectively. The whole-cell biotransformation platform effectively leverages the regio-selectivity of UGT78A11 and UGT88D7 to convert polyhydroxy secondary metabolites into monoglucuronides with promising yields, thereby enhancing the availability and physiological potential of these glucuronides.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"954-964"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of the microalga Pavlova sp. OPMS 30543 with diclofenac attenuates hyperalgesia in adjuvant-induced arthritis mice. 微藻Pavlova sp. OPMS 30543与双氯芬酸联合使用可减轻佐剂性关节炎小鼠的痛症过敏。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf047

Wataru Nemoto, Kotaro Yamada, Ruka Kobayashi, Tomohiro Hoshi, Ken Nakahara, Akihiko Kanamoto, Osamu Nakagawasai, Koichi Tan-No

引用次数: 0

Downsizing effect on direct electron transfer-type bioelectrocatalysis by d-fructose dehydrogenase with structural insight. d-果糖脱氢酶对直接电子转移型生物电催化的缩小效应及其结构研究。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf043

Yohei Suzuki, Yuki Kitazumi, Osamu Shirai, Keisei Sowa

引用次数: 0

Intracellular electron donor stripping to identify electron donors of indigo reduction. 胞内电子给体剥离鉴定靛蓝还原的电子给体。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf054

Hiroki Tanaka, Mayu Kikuchi, Kenji Kano, Eiji Sakuradani, Jun Ogawa, Kouichi Kuroda, Michiki Takeuchi

引用次数: 0

Identification of peptaibols as specific inhibitors of the futalosine pathway. 富塔苷途径特异性抑制肽的鉴定。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf050

Marie Kikuchi, Haruka Kiyanagi, Takeshi Tsunoda, Yuki Inahashi, Kenichi Nonaka, Tohru Dairi, Yasushi Ogasawara

引用次数: 0

Expression in HEK293 cells, purification, and characterization of recombinant human α-glucosidases. 重组人α-葡萄糖苷酶在HEK293细胞中的表达、纯化和表征。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf055

So Nishimoto, Teisuke Takita, Kazumichi M Nishida, Masaaki Ito, Toshiyuki Kimura, Yasuhisa Kimura, Noriyuki Kioka, Kiyoshi Yasukawa

引用次数: 0