Bioscience, Biotechnology, and Biochemistry最新文献_第10页

Dual-faceted regulation by butyrophilin 1A1 to produce MUC5AC and IL-8 from human colonic cell line HT29-MTX-E12. 嗜丁酸蛋白1A1对人结肠细胞系HT29-MTX-E12产生MUC5AC和IL-8的双重调控

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag072

Minori Ishida, Yuki Kuwana, Mizuki Tsujimoto, Hyejin Namgung, Ayami Furue, Fumito Tani

{"title":"Dual-faceted regulation by butyrophilin 1A1 to produce MUC5AC and IL-8 from human colonic cell line HT29-MTX-E12.","authors":"Minori Ishida, Yuki Kuwana, Mizuki Tsujimoto, Hyejin Namgung, Ayami Furue, Fumito Tani","doi":"10.1093/bbb/zbag072","DOIUrl":"10.1093/bbb/zbag072","url":null,"abstract":"Butyrophilin subfamily 1 member A1 (BTN1A1) is a major protein of the milk fat globule membrane, but its immunoregulatory function remains largely unresolved, despite its structural similarity to the B7 co-stimulatory molecules. In this study, we investigated the immunological significance of BTN1A1 in gastrointestinal epithelial cells. The human ectodomain of BTN1A1 expressed as a soluble Fc fusion protein (ectoBTN-Fc) was added to HT29-MTX-E12 cells as a model for gastrointestinal epithelial cells. In the absence of tumor necrosis factor (TNF)-α, it significantly promoted MUC5AC production via a weak activation of NF-κB signal transduction pathway. In contrast, prior incubation with ectoBTN-Fc inhibited TNF-α-provoked-IL-8 secretion by suppressing the phosphorylation of NF-κB p65. Binding analyses demonstrated that ectoBTN-Fc likely binds to HT29-MTX-E12 cells, followed by its endocytosis. These results suggest a dual-faceted regulatory function of BTN1A1. It reinforces intestinal mucosal barrier function under basal conditions, while it may endow a prophylactic effect alleviating strong inflammatory stimulation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1007-1018"},"PeriodicalIF":1.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recrystallization-induced tautomerism and selective imine formation of N-diphenylmethylene-serine methyl ester. n-二苯基亚甲基丝氨酸甲酯重结晶诱导的互变异构和选择性亚胺生成。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag068

Resky Nugraha, Fumiya Nakayama, Desita Triana Aziz, Sumayya Binti Abd Azis, Marwah Wirda Ningsih, Yuki Fukuda, Zetryana Puteri Tachrim, Tsunayoshi Takehara, Takeyuki Suzuki, Yuta Murai, Makoto Hashimoto

{"title":"Recrystallization-induced tautomerism and selective imine formation of N-diphenylmethylene-serine methyl ester.","authors":"Resky Nugraha, Fumiya Nakayama, Desita Triana Aziz, Sumayya Binti Abd Azis, Marwah Wirda Ningsih, Yuki Fukuda, Zetryana Puteri Tachrim, Tsunayoshi Takehara, Takeyuki Suzuki, Yuta Murai, Makoto Hashimoto","doi":"10.1093/bbb/zbag068","DOIUrl":"10.1093/bbb/zbag068","url":null,"abstract":"Diphenylmethylene serine methyl ester is an important precursor for the synthesis of O-glycoprotein mimics; however, it exists as an equilibrium mixture of imine and oxazolidine forms due to intramolecular cyclization. Upon crystallization from ether/hexane, the oxazolidine form is typically obtained as the predominant species, and the pure imine form has not been isolated to date. In this study, diphenylmethylene serine methyl ester was synthesized from serine methyl ester and benzophenone imine, purified by chromatography, and subsequently recrystallized from methanol to selectively afford the imine form in high purity. The structure was confirmed by nuclear magnetic resonance spectroscopy and single-crystal X-ray diffraction analysis. Tautomeric interconversion was investigated in deuterated chloroform at various temperatures, revealing a gradual conversion of the imine into the oxazolidine form until equilibrium was established. These findings clarify the tautomeric behavior of this compound and provide an effective strategy for the selective isolation of tautomers of serine derivatives.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"992-1000"},"PeriodicalIF":1.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thymol gel ameliorates anesthesia-related postoperative neuropathic pain: modulation of inflammatory mediators in a rat model. 百里香酚凝胶改善麻醉相关术后神经性疼痛：炎症介质在大鼠模型中的调节。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag071

Hu Fan, Wenfeng Xiong, Liming Liu, Chengzhe Zhang, Yunjia Hu, Lili Zhang, Hemant Kamble, Xiaojun Liang, Zhenzhou He, Qingqing Fan

{"title":"Thymol gel ameliorates anesthesia-related postoperative neuropathic pain: modulation of inflammatory mediators in a rat model.","authors":"Hu Fan, Wenfeng Xiong, Liming Liu, Chengzhe Zhang, Yunjia Hu, Lili Zhang, Hemant Kamble, Xiaojun Liang, Zhenzhou He, Qingqing Fan","doi":"10.1093/bbb/zbag071","DOIUrl":"10.1093/bbb/zbag071","url":null,"abstract":"This study investigated the molecular mechanisms and therapeutic efficacy of chitosan-based thymol gels (2.5%, 5%, and 10%) in postoperative neuropathic pain. Gels exhibited pseudoplastic shear-thinning behavior and a biphasic release profile, achieving > 90% cumulative release within 12 h. Using a Wistar rat model of hind paw incision, topical treatment with 5% and 10% thymol gels significantly (P < .05) reversed the reduction in paw withdrawal latency and threshold compared to the controls. Thymol gel markedly decreased serum corticosterone, cyclooxygenase-2 (COX-II), and prostaglandin E2 (PGE2) levels. Furthermore, it downregulated the messenger ribonucleic acid (mRNA) expression of interleukins and tumor necrosis factor in the sciatic nerve. Histopathological analysis confirmed reduced inflammation and congestion. Positive correlations were observed between pro-inflammatory cytokine expression and serum corticosterone, PGE2, and COX-II levels. These findings suggest that thymol gel effectively ameliorates anesthesia-related postoperative neuropathic pain by modulating key inflammatory mediators, suggesting its beneficial treatment option for alleviating such pain.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1044-1054"},"PeriodicalIF":1.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147970503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of β-glucan nanocarriers for enhanced encapsulation and delivery of doxorubicin for triple negative breast cancer treatment. β-葡聚糖纳米载体的制备用于阿霉素三阴性乳腺癌治疗的增强包封和递送。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag066

Morteza Servatan, Fatma Zeynep Bozkurt, Zeynep Yilmazer Hitit, Birgütay Şahin, Damla Nur Parıltı, Suna Ertunç, Pelin Mutlu

引用次数: 0

Antimelanogenic properties of glycerol-linked hexadecatetraenoic acid derivatives isolated from Ulva prolifera. 从浒苔中分离的甘油连接的十六烷基四烯酸衍生物的抗黑素特性。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag065

Yuzuki Yamada, Togo Shimomura, Marimo Miyazono, Shiho Hagihara, Makoto Nakaya, Shunta Sakamoto, Kenji Kai, Satoshi Handa, Yushi Hashizume, Tandia Mahamadou, Tatsuji Sakamoto

{"title":"Antimelanogenic properties of glycerol-linked hexadecatetraenoic acid derivatives isolated from Ulva prolifera.","authors":"Yuzuki Yamada, Togo Shimomura, Marimo Miyazono, Shiho Hagihara, Makoto Nakaya, Shunta Sakamoto, Kenji Kai, Satoshi Handa, Yushi Hashizume, Tandia Mahamadou, Tatsuji Sakamoto","doi":"10.1093/bbb/zbag065","DOIUrl":"10.1093/bbb/zbag065","url":null,"abstract":"Ethanol extracts from four species of green algae were comparatively evaluated for antimelanogenic activity using B16F10 mouse melanoma cells. The extract derived from Ulva prolifera demonstrated the strongest inhibitory effect on melanin synthesis and was thus selected for further investigation to isolate and identify its bioactive constituents. Through sequential chromatographic techniques, two active compounds-1-hexadecatetraenoylglycerol and 1-hexadecatetraenoyl-3-β-D-galactopyranosylglycerol-were isolated and structurally characterized via NMR and mass spectrometric analyses. Notably, hexadecatetraenoic acid alone did not exhibit antimelanogenic activity under the same experimental conditions, whereas its glycerol-conjugated form, 1-hexadecatetraenoylglycerol, significantly suppressed melanin production. Additionally, neither the ethanol extract of U. prolifera nor the isolated compounds directly inhibited tyrosinase activity, suggesting that they may influence melanogenesis through indirect mechanisms. These results suggest that the molecular configuration of fatty acids plays an important role in antimelanogenic activity and highlight the potential of lipid components from U. prolifera as functional antimelanogenic agents.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"977-985"},"PeriodicalIF":1.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the biosynthetic gene cluster for PF1451A: implications for untapped peptide biosynthetic pathways widely distributed in fungi. PF1451A生物合成基因簇的鉴定：对广泛分布于真菌中的未开发肽生物合成途径的意义。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag074

Genki Hibi, Taro Ozaki, Riku Sugimoto, Yohei Morishita, Teigo Asai

引用次数: 0

Tissue-specific and environmental regulation of ascorbate accumulation in the cactus Nopalea cochenillifera. 仙人掌抗坏血酸积累的组织特异性和环境调控。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag076

Qiaochu Wang, Shuusuke Kawachi, Kota Handa, Kazuya Yoshimura

{"title":"Tissue-specific and environmental regulation of ascorbate accumulation in the cactus Nopalea cochenillifera.","authors":"Qiaochu Wang, Shuusuke Kawachi, Kota Handa, Kazuya Yoshimura","doi":"10.1093/bbb/zbag076","DOIUrl":"10.1093/bbb/zbag076","url":null,"abstract":"Cacti are xerophytic plants characterized by water-storing cladodes, thick cuticles, and CAM photosynthesis, enabling high productivity under harsh conditions. They accumulate ascorbate (AsA) at levels comparable to those of leafy vegetables, potentially contributing to stress tolerance and nutritional value. However, the regulation of AsA accumulation in cacti remains poorly understood. Here, we used the dwarf prickly pear cactus Nopalea cochenillifera to investigate the tissue-specific distribution and environmental responses of AsA. AsA preferentially accumulated in the cortex, exhibited light-dependent increases, and was maintained or even increased under high-light, drought, and low-temperature stress conditions. Supplementation experiments with AsA and its precursors suggest the presence of multiple biosynthetic pathways and inter-cladode transport mechanisms. These results provide insights into mechanisms specific to the cactus N. cochenillifera for maintaining AsA homeostasis under adverse environmental conditions. This study provides the first evidence of tissue-specific and stress-responsive regulation of AsA metabolism in cactus cladodes.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1072-1082"},"PeriodicalIF":1.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roquefortine C isolated from a quail feces-derived fungus exhibits aryl hydrocarbon receptor antagonistic and broad-spectrum antiviral activities. 从鹌鹑粪源真菌中分离得到的洛克福汀C具有芳烃受体拮抗和广谱抗病毒活性。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-23 DOI: 10.1093/bbb/zbag070

Karin Maruyama, Kou Nakamura, Mitsuki Yasukochi, Hirofumi Ohashi, Shogo Nakajima, Shiki Saitoh, Nozomi Mosu, Namiho Suzuki, Chihiro Kase, Naoyuki Aihara, Hironobu Murakami, Maiko Okada, Kan Fujino, Koichi Watashi, Shinji Kamisuki

{"title":"Roquefortine C isolated from a quail feces-derived fungus exhibits aryl hydrocarbon receptor antagonistic and broad-spectrum antiviral activities.","authors":"Karin Maruyama, Kou Nakamura, Mitsuki Yasukochi, Hirofumi Ohashi, Shogo Nakajima, Shiki Saitoh, Nozomi Mosu, Namiho Suzuki, Chihiro Kase, Naoyuki Aihara, Hironobu Murakami, Maiko Okada, Kan Fujino, Koichi Watashi, Shinji Kamisuki","doi":"10.1093/bbb/zbag070","DOIUrl":"10.1093/bbb/zbag070","url":null,"abstract":"Aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, regulates the metabolism of exogenous compounds. Pharmacological inhibition of AhR reduces lipid droplet (LD) accumulation and suppresses hepatitis C virus (HCV) proliferation. Recent evidence implicates AhR in the propagation of viruses such as herpes simplex virus type 1 (HSV-1), making it a host factor regulating the proliferation of diverse viruses and an attractive target for broad-spectrum antivirals. To identify AhR ligands with antiviral activity, we screened secondary metabolites derived from fungi isolated from various animals. We identified roquefortine C, an indole alkaloid obtained from quail-derived fungi, as an AhR antagonist. Roquefortine C suppressed LD formation and HCV production in human hepatoma cells. Additionally, it showed anti-HSV-1 and anti-pseudorabies virus (PRV) activities. Notably, our study demonstrated the relationship between AhR and PRV for the first time. AhR ligands could represent potential lead compounds with broad-spectrum antiviral properties.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"986-991"},"PeriodicalIF":1.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium tuberculosis shikimate kinase inhibitory activity of a glycodidepside mixture from Cosmospora sp. BioMCC-f.T-7332. cosmspora sp. biomcc -f - t -7332中糖二dep苷混合物对结核分枝杆菌莽草酸激酶抑制活性的研究。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-07-14 DOI: 10.1093/bbb/zbag103

Eka Siska, Novriyandi Hanif, Gustini Syahbirin, Erwahyuni Endang Prabandari, Danang Waluyo, Titin Ariyani, Amila Pramisandi, Diana Dewi, Arif Nurkanto, Kin-Ichi Oyama, Mihoko Mori, Tomoyoshi Nozaki

引用次数: 0

Exposure to furaneol odor affects spontaneous locomotor activity in mice. 暴露于呋喃醇气味影响小鼠的自发运动活动。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-06-23 DOI: 10.1093/bbb/zbag056

Motoko Ohata, Momoka Kinowaki, Kenta Sato, Issei Yokoyama, Eri Kakimoto, Kazumi Osada

引用次数: 0