Bioscience, Biotechnology, and Biochemistry最新文献_第10页

New abietane diterpenes from Origanum vulgare exhibiting lipase inhibitory activity. 具有脂肪酶抑制活性的新戊二萜。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag046

Yu Komata, Ayano Watanabe, Eri Fukushi, Masaru Hashimoto, Jing Wu, Hirokazu Kawagishi, Naoki Kitaoka, Hideyuki Matsuura, Chika Kitaoka-Saito, Kosaku Takahashi

{"title":"New abietane diterpenes from Origanum vulgare exhibiting lipase inhibitory activity.","authors":"Yu Komata, Ayano Watanabe, Eri Fukushi, Masaru Hashimoto, Jing Wu, Hirokazu Kawagishi, Naoki Kitaoka, Hideyuki Matsuura, Chika Kitaoka-Saito, Kosaku Takahashi","doi":"10.1093/bbb/zbag046","DOIUrl":"10.1093/bbb/zbag046","url":null,"abstract":"Many herbs and spices have been reported to exhibit various biological activities, including antioxidant effects, antimicrobial activity, and antitumor activity; consequently, they are expected to contain novel bioactive compounds. In this study, antiobesity agents were identified in several herbs and spices by screening for inhibitors of lipase, a key enzyme involved in lipid digestion. The extracts of Origanum vulgare (oregano) demonstrated considerable lipase inhibitory activity. The active constituents from this plant were isolated by subjecting the oregano extract to several separation protocols, including SiO2 gel column chromatography and reversed-phase high-performance liquid chromatography. The purification process was guided by lipase inhibitory activity, affording origanone A (1) and origanone B (2) as novel abietane diterpenes. Notably, these compounds exhibited moderate lipase inhibitory activity. These findings indicate the potential of oregano and its active constituents as functional food ingredients for regulating lipid levels and obesity.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"729-735"},"PeriodicalIF":1.5,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147526938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential nitrogen-fixing endophytic bacteria in hydroponically grown sweet potato with low nitrogen fertilization revealed by 16S rRNA gene amplicon sequencing. 16S rRNA基因扩增子测序揭示低氮条件下水培甘薯潜在的固氮内生细菌

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag036

Masanori Takamura

引用次数: 0

Characterization of bacterial l-galactose dehydrogenase with l-glucose dehydrogenase activity from Luteolibacter sp. strain LG18. Luteolibacter sp.菌株LG18细菌l-半乳糖脱氢酶与l-葡萄糖脱氢酶活性的研究

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag041

Kairi Koubara, Myoungjin Kim, Mihoko Takenoya, Aoi Nakanishi, Mayu Suzuki, Shion Azuma, Shinsaku Ito, Yasuyuki Sasaki, Akira Nakamura, Shunsuke Yajima

{"title":"Characterization of bacterial l-galactose dehydrogenase with l-glucose dehydrogenase activity from Luteolibacter sp. strain LG18.","authors":"Kairi Koubara, Myoungjin Kim, Mihoko Takenoya, Aoi Nakanishi, Mayu Suzuki, Shion Azuma, Shinsaku Ito, Yasuyuki Sasaki, Akira Nakamura, Shunsuke Yajima","doi":"10.1093/bbb/zbag041","DOIUrl":"10.1093/bbb/zbag041","url":null,"abstract":"The crystal structure of l-galactose dehydrogenase (LGDH) with l-glucose dehydrogenase activity from Luteolibacter sp. strain LG18 (Lu-LGDH) was determined in complex with l-galactose or l-glucose and NADP⁺. This structural analysis identified key residues involved in substrate binding, and alanine-substituted mutants demonstrated the roles of these residues, including Tyr56, acting as a potential general base within the catalytic tetrad. Unlike plant enzymes that show a preference for NAD⁺, Lu-LGDH exhibits a marked preference for NADP⁺ as a cofactor. This preference was attributed to the interaction of the phosphate group with Arg28, Thr269, and Asn274. The binding mode of l-glucose was similar to that of l-galactose. The C4 hydroxyl group (the structural difference between these pyranoses) was not used for substrate binding, which explains the dual activity of the enzyme. Furthermore, among the substrate-binding residues that were mutated, Arg308, which is not conserved among LGDHs, was crucial for the enzymatic activity.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"746-754"},"PeriodicalIF":1.5,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of blastmycetin G, a sofa-conformation fixed analog of teleocidin B's, produced by Streptomyces blastmyceticus. blastmycetin G是由blastmyceticus链霉菌产生的一种软构象固定类似物。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag044

Shin-Ichiro Kajiyama, Ryo C Yanagita, Chihiro Tsukano, Kazuhiro Irie

引用次数: 0

Functional analysis of CAPRICE S1 motif residues via alanine scanning in Arabidopsis. 拟南芥CAPRICE S1基序残基的丙氨酸扫描功能分析。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag039

Arata Idogawa, Juri Wakamatsu, Souma Matsuzaka, Hiroki Natsume, Sotaro Fujii, Rumi Tominaga

引用次数: 0

Structure-guided design and rapid cell-free screening of a superior restriction endonuclease DpnI mutant to enhance the specific activity and efficiency of site-directed mutagenesis. 结构引导设计和快速无细胞筛选一个优越的限制性内切酶DpnI突变体，以提高定点突变的特异性活性和效率。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag038

Dan Zhang, Hongjie Ren, Yiyang Wang, Ning Jin, Xiuyu Feng, Meiqi Liu, Zhidan Luo, Chen Lu

引用次数: 0

Excavation of Lentinula edodes-stem-derived peptides doubly inhibiting DPP-IV and ACE activity. 香菇茎源多肽双抑制DPP-IV和ACE活性的挖掘。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag026

Qiulan Zheng, Qian Kang, Haosu Zhang, Lega Tu, Yuanshan Yu, Jiguo Yang, Yuqian Tang

引用次数: 0

Structure-guided identification of dipeptides modulating PPARα-associated lipid metabolism. 结构引导鉴定调节ppar α相关脂质代谢的二肽。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag037

Arata Banno, Rana Yu, Nanami Nishino, Mako Yamamoto, Asahi Takeuchi, Natsuki Oda, Akio Ebihara, Satoshi Nagaoka

引用次数: 0

Influence of dietary fiber fermentability on DSS-induced colitis severity and muscle wasting via gut microbiota. 膳食纤维发酵性通过肠道菌群对dss诱导的结肠炎严重程度和肌肉萎缩的影响

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag035

Takafumi Aoki, Masato Kawashima, Eri Oyanagi, Chihiro Watanabe, Hiroki Hamada, Takumi Kono, Michael J Kremenik, Hiromi Yano

{"title":"Influence of dietary fiber fermentability on DSS-induced colitis severity and muscle wasting via gut microbiota.","authors":"Takafumi Aoki, Masato Kawashima, Eri Oyanagi, Chihiro Watanabe, Hiroki Hamada, Takumi Kono, Michael J Kremenik, Hiromi Yano","doi":"10.1093/bbb/zbag035","DOIUrl":"10.1093/bbb/zbag035","url":null,"abstract":"Dietary fiber may improve dysbiosis and contribute to the management of intestinal inflammation and muscle wasting. We examined whether low- and high-fermentable fibers differently influence dextran sulfate sodium (DSS)-induced colitis severity and muscle wasting. Male C57BL/6 J mice were assigned to four groups: nonfiber control (N), nonfiber with DSS (ND), cellulose with DSS (CD), and partially hydrolyzed guar-gum with DSS (GD), then subjected to a 27-day DSS protocol. Colitis severity was attenuated in the CD group, accompanied by increased Lactobacillus and Lactococcus lactis and decreased Clostridium innocuum group. These microbial changes were associated with maintenance of gastrocnemius muscle mass through mitochondrial biogenesis. Conversely, the GD group exhibited exacerbated colitis, associated with increased cecal succinate and expansion of Bacteroides, Blautia, and Enterococcaceae. These alterations correlated with muscle wasting accompanied by mitochondrial dysfunction. These results suggest that fiber fermentability plays a pivotal role in colitis management via gut microbiota alterations, also associated with muscle wasting.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"804-814"},"PeriodicalIF":1.5,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species-specific deglucuronidation of quercetin glucuronides by the human gut microbiota. 人类肠道菌群对槲皮素葡糖醛酸酯的物种特异性去糖醛酸化。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-05-20 DOI: 10.1093/bbb/zbag032

Mst Julia Sultana, Yuta Yoshida, Shin Kurihara, Miyu Nishikawa, Shinichi Ikushiro

{"title":"Species-specific deglucuronidation of quercetin glucuronides by the human gut microbiota.","authors":"Mst Julia Sultana, Yuta Yoshida, Shin Kurihara, Miyu Nishikawa, Shinichi Ikushiro","doi":"10.1093/bbb/zbag032","DOIUrl":"10.1093/bbb/zbag032","url":null,"abstract":"The gut microbiota plays a pivotal role in human health and interacts with quercetin, which undergoes extensive glucuronidation in the host organism. Microbial β-glucuronidases can deconjugate quercetin monoglucuronides, regenerating the aglycone and modulating bioavailability; however, the magnitude and drivers of inter-individual variability remain unclear. We assembled a panel of human-derived intestinal isolates and quantified the deglucuronidation of four regioisomeric quercetin monoglucuronides using intact whole cells. Deglucuronidation capacity varied by approximately an order of magnitude across taxa and depended on the conjugation site: A/C-ring glucuronides were generally more labile than B-ring forms, with some species-specific exceptions. Notably, Faecalibacterium duncaniae emerged as a high-efficiency, broad-coverage deglucuronidator, outperforming the other isolates across all substrates. Whole-community assays of cecal content and fecal pellets echoed these preferences and revealed higher activity in digesta. These findings delineate species-resolved deglucuronidation patterns underlying inter-individual differences in quercetin bioavailability and identify species microbiota-driven deconjugation into pharmacokinetic models.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"821-831"},"PeriodicalIF":1.5,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147316393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0