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A unique structure of bacteriophage T4 gene 32 protein with double-stranded DNA in low-salt conditions is distinguished by antibodies. 噬菌体 T4 基因 32 蛋白与双链 DNA 在低盐条件下的独特结构可通过抗体区分。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf009
Mina Yasuda, Ngan Thi Kim Pham, Yuki Hirakawa, Keiko Momma, Teisuke Takita, Makoto Tsuboi, Kiyoshi Yasukawa, Kazuaki Yoshimune
{"title":"A unique structure of bacteriophage T4 gene 32 protein with double-stranded DNA in low-salt conditions is distinguished by antibodies.","authors":"Mina Yasuda, Ngan Thi Kim Pham, Yuki Hirakawa, Keiko Momma, Teisuke Takita, Makoto Tsuboi, Kiyoshi Yasukawa, Kazuaki Yoshimune","doi":"10.1093/bbb/zbaf009","DOIUrl":"10.1093/bbb/zbaf009","url":null,"abstract":"<p><p>Bacteriophage T4 gene 32 protein (gp32) preferentially binds to single-stranded DNA (ssDNA) to facilitate DNA replication but shows weak binding to double-stranded DNA (dsDNA). Polyclonal and monoclonal antibodies against gp32 were raised, and an enzyme-linked immunosorbent assay was used to evaluate their reactivities against gp32. The reactivity of the monoclonal antibody MGP45 was diminished in the presence of 5 ng/mL dsDNA, suggesting a conformational change that reduces epitope availability. Notably, the same concentration of ssDNA had little effect; instead, 500 ng/mL ssDNA was required to elicit the same degree of inhibition. A decrease in MGP45 reactivity with gp32 was observed in the presence of NaCl at concentrations less than 100 m m under neutral conditions. These changes in antibody reactivity reflect differences in the gp32 conformation, which may underlie its different affinities for ssDNA and dsDNA.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"728-732"},"PeriodicalIF":1.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological and biochemical studies on cell surface functions in microorganisms used in brewing and fermentation industry. 酿造和发酵工业中微生物细胞表面功能的生物学和生化研究。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-22 DOI: 10.1093/bbb/zbaf020
Keietsu Abe
{"title":"Biological and biochemical studies on cell surface functions in microorganisms used in brewing and fermentation industry.","authors":"Keietsu Abe","doi":"10.1093/bbb/zbaf020","DOIUrl":"10.1093/bbb/zbaf020","url":null,"abstract":"<p><p>When brewing microorganisms, which include bacteria and fungi, act on solid cereal substrates, the microbial cell surface interacts with the substrate. When microorganisms use sugars and amino acids released by hydrolysis of the substrate, this occurs on the cell surface. Throughout my career, I have focused on functional studies of cell surface molecules such as solute transporters, cell wall components, and bio-surfactants and applied the knowledge obtained to the development of fermentation technologies. In this review, I describe (i) catabolite control by sugar transporters and energy generation coupled with amino acid decarboxylation in lactic acid bacteria; (ii) recruitment of a polyesterase by the fungal bio-surfactant proteins to polyesters and subsequent promotion of polyester hydrolysis; and (iii) hyphal aggregation via cell wall α-1,3-glucan and galactosaminogalactan in aspergilli and the development of a novel liquid culture method with hyphal dispersed mutants lacking these two polysaccharides.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"649-667"},"PeriodicalIF":1.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liraglutide enhances myotube differentiation and muscle contractile activity upon electric pulse stimulation in mouse skeletal muscle cells. 利拉鲁肽增强电脉冲刺激小鼠骨骼肌细胞的肌管分化和肌肉收缩活性。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-21 DOI: 10.1093/bbb/zbaf060
Risa Mukai, Ayumu Kojima, Mizuki Morisasa, Wakako Tawara, Tsukasa Mori, Naoko Goto-Inoue
{"title":"Liraglutide enhances myotube differentiation and muscle contractile activity upon electric pulse stimulation in mouse skeletal muscle cells.","authors":"Risa Mukai, Ayumu Kojima, Mizuki Morisasa, Wakako Tawara, Tsukasa Mori, Naoko Goto-Inoue","doi":"10.1093/bbb/zbaf060","DOIUrl":"https://doi.org/10.1093/bbb/zbaf060","url":null,"abstract":"<p><p>Glucagon-like peptide-1 (GLP-1) is a potent incretin hormone produced by L-cells in the ileum and colon. Skeletal muscle, the most important organ for glucose metabolism, is also affected by GLP-1. Short-term administration of liraglutide, a GLP-1 analog, ameliorated glucose uptake in palmitate-treated type II diabetes models; however, the influence of long-term liraglutide administration on normal muscle cells has not been evaluated. We analyzed the effects of chronic (3 consecutive days) administration of liraglutide at various concentrations on healthy C2C12 skeletal muscle cells by investigating morphological changes, muscle contractile properties, and glucose uptake. Liraglutide administration at an appropriate dose (0.5 μM) had positive effects, including the promotion of muscle hypertrophy, in C2C12 cells; however, excessive administration (10 μM) had atrophic effects. Therefore, proper liraglutide dosing is very important.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Total synthesis and structure determination of ephedroidin. 麻黄素的全合成及结构测定。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-04-17 DOI: 10.1093/bbb/zbaf059
Ami Sakurai, Atsushi Kawamura, Yasunao Hattori, Hidefumi Makabe
{"title":"Total synthesis and structure determination of ephedroidin.","authors":"Ami Sakurai, Atsushi Kawamura, Yasunao Hattori, Hidefumi Makabe","doi":"10.1093/bbb/zbaf059","DOIUrl":"https://doi.org/10.1093/bbb/zbaf059","url":null,"abstract":"<p><p>The total synthesis and structure determination of enantiomerically pure (+)- and (-)-ephedroidin from the leguminous plant, is described. (+)- and (-)-Ephedroidin were a flavonoid with a chiral secondary alcohol on a side chain derived from a prenyl group. These compounds were obtained by optical resolution of (±)-ephedroidin through α-methoxyphenylacetic acid (MPA) esterification. The racemic secondary hydroxy group of (±)-ephedroidin was generated by photooxidation of prenylated flavonoid. Based on the total synthesis and Trost's method using α-methoxyphenylacetic acid (MPA) ester derivatives, the absolute configurations of (+)- and (-)-ephedroidin were revealed.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oral administration of astaxanthin mitigates chronological skin aging in mice. 口服虾青素可减轻小鼠的皮肤老化。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae205
Shuyu Liu, Yuki Manabe, Tatsuya Sugawara
{"title":"Oral administration of astaxanthin mitigates chronological skin aging in mice.","authors":"Shuyu Liu, Yuki Manabe, Tatsuya Sugawara","doi":"10.1093/bbb/zbae205","DOIUrl":"10.1093/bbb/zbae205","url":null,"abstract":"<p><p>Intrinsic skin aging is a chronological decline in skin texture and function influenced largely by genetic factors. Aged skin exhibits morphological alterations, including wrinkling, dryness, and roughness, along with dysfunctional changes in the skin barrier. In this study, the in vivo anti-intrinsic aging efficacy of dietary astaxanthin extracted from Haematococcus pluvialis on the skin was evaluated using aged C57BL/6 J mice. As a result, dietary supplementation of 0.1% astaxanthin significantly alleviated the defects in skin's water retention capacity, viscoelasticity, and reduced wrinkle formation induced by intrinsic aging. Furthermore, gene expression analysis revealed that dietary astaxanthin was capable of mediating genes related to the proliferation and differentiation of skin cells, degradation of proteins in the extracellular matrix and dermal-epidermal junction, synthesis of natural moisturizing factors, and maintenance of skin barrier function. Together, our data indicate that dietary astaxanthin has potential applications as a novel ingredient in nutricosmetics against chronological skin aging.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"612-621"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Co-culture of Aspergillus niger IFM 59706 and RAW264 cells enhances the production of aurasperone A with nitric oxide inhibitory activity. 黑曲霉IFM 59706细胞与RAW264细胞共培养可提高aurasperone A的产量，并具有NO抑制活性。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae211
Yukiko Ujie, Shun Saito, Tomoya Banno, Takashi Yaguchi, Midori A Arai
{"title":"Co-culture of Aspergillus niger IFM 59706 and RAW264 cells enhances the production of aurasperone A with nitric oxide inhibitory activity.","authors":"Yukiko Ujie, Shun Saito, Tomoya Banno, Takashi Yaguchi, Midori A Arai","doi":"10.1093/bbb/zbae211","DOIUrl":"10.1093/bbb/zbae211","url":null,"abstract":"<p><p>Most actinomycetes and fungi have a multitude of silent biosynthetic genes whose activation could lead to the production of new natural products. Our group recently designed and used a co-culture method to isolate new natural products, based on the idea that pathogens might produce immune suppressors to avoid attack by immune cells. Here, we searched for compounds produced by the co-culture of immune cells with pathogenic fungi isolated from clinical specimens. The production of dimeric naphtho-γ-pyrone aurasperone A (1) was enhanced by the co-culture of pathogenic fungus Aspergillus niger IFM 59706 and RAW264 mouse macrophage-like cells. The absolute configuration of 1 was confirmed by comparison with the reported electronic circular dichroism spectrum. This is the first report of the inhibitory activity of 1 on nitric oxide production, an inflammatory mediator.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"541-547"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of microemulsion system on water dispersibility and bioavailability of γ-oryzanol. 微乳液体系对γ-谷维醇水分散性和生物利用度的影响。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbaf002
Junya Ito, Naoko Kumagai, Ayaka Suzuki, Naoki Shoji, Isabella Supardi Parida, Mamoru Takahashi, Kiyotaka Nakagawa
{"title":"Effect of microemulsion system on water dispersibility and bioavailability of γ-oryzanol.","authors":"Junya Ito, Naoko Kumagai, Ayaka Suzuki, Naoki Shoji, Isabella Supardi Parida, Mamoru Takahashi, Kiyotaka Nakagawa","doi":"10.1093/bbb/zbaf002","DOIUrl":"10.1093/bbb/zbaf002","url":null,"abstract":"<p><p>This study developed water-dispersible γ-oryzanol (WD-OZ) using a microemulsion system and assessed their absorption in rats. While OZ itself is hardly soluble in water, WD-OZ exhibited high water dispersibility, and OZ, along with its metabolites, was detected in rat plasma. These findings provide a solid basis for future application of the microemulsion-based approach to enhance the bioavailability of OZ in food.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"633-637"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anaerobic plasmalogen production in recombinant Escherichia coli carrying plasmalogen synthase gene from Selenomonas ruminantium. 携带反刍硒单胞菌质酵素合成酶基因的重组大肠杆菌厌氧产质酵素。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae208
Naoki Yoshii, Keita Higuchi, Tomoko Onodera, Naoki Abe, Jun Kaneko
{"title":"Anaerobic plasmalogen production in recombinant Escherichia coli carrying plasmalogen synthase gene from Selenomonas ruminantium.","authors":"Naoki Yoshii, Keita Higuchi, Tomoko Onodera, Naoki Abe, Jun Kaneko","doi":"10.1093/bbb/zbae208","DOIUrl":"10.1093/bbb/zbae208","url":null,"abstract":"<p><p>Escherichia coli expressing SrPlsAR from Selenomonas ruminantium produces plasmalogen, comprising almost 60% of the total phospholipid content under anaerobic conditions. Both plasmenylethanolamine and plasmenylglycerol were detected, and the major acyl aldehyde derived from sn-1 vinyl ether was C16:1. Plasmalogen synthesis is affected by mutations in ATP-binding sites and Cys is expected to be involved in the formation of the [4Fe-4S] cluster.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"594-598"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FRPR-1, a G protein-coupled receptor in the FMRFamide-related peptide receptor family, modulates larval development as a receptor candidate of the FMRFamide-like peptide FLP-1 in Caenorhabditis elegans. FRPR-1是fmrfamily相关肽受体家族中的G蛋白偶联受体（GPCR），在秀丽隐杆线虫中作为fmrfamily样肽FLP-1的候选受体调节幼虫的发育。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbaf004
Risako Une, Riko Uegaki, Sho Maega, Masahiro Ono, Tomohiro Bito, Takashi Iwasaki, Akira Shiraishi, Honoo Satake, Tsuyoshi Kawano
{"title":"FRPR-1, a G protein-coupled receptor in the FMRFamide-related peptide receptor family, modulates larval development as a receptor candidate of the FMRFamide-like peptide FLP-1 in Caenorhabditis elegans.","authors":"Risako Une, Riko Uegaki, Sho Maega, Masahiro Ono, Tomohiro Bito, Takashi Iwasaki, Akira Shiraishi, Honoo Satake, Tsuyoshi Kawano","doi":"10.1093/bbb/zbaf004","DOIUrl":"10.1093/bbb/zbaf004","url":null,"abstract":"<p><p>FMRFamide-like peptides (FLPs) and their receptors, FMRFamide-related peptide receptors (FRPRs) are widely conserved in free-living and parasitic nematodes. Herein, we identified FRPR-1 as an FLP-1 receptor candidate involved in larval development and diapause in the model nematode Caenorhabditis elegans. Our molecular genetic study, supported by in silico research, revealed the following: (1) frpr-1 loss-of-function completely suppresses the promotion of larval diapause caused by flp-1 overexpression; (2) AlphaFold2 analysis revealed the binding of FLP-1 to FRPR-1; (3) FRPR-1 as well as FLP-1 modulates the production and secretion of the predominant insulin-like peptide DAF-28, which is produced in ASI neurons; and (4) the suppression of larval diapause by frpr-1 loss-of-function is completely suppressed by a daf-28 defect. Thus, FRPR-1 regulates larval development and diapause by modulating DAF-28 production and secretion. This study may provide new insights into the development of novel nematicides targeting parasitic nematodes using FRPR-1 inhibitors.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"586-593"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toward nanoscale structural and chemical analysis of microbial surfaces. 实现微生物表面的纳米级结构和化学分析。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae176
Ryo Kato, Keisuke Miyazawa, Takumi Imura, Takeo Minamikawa
{"title":"Toward nanoscale structural and chemical analysis of microbial surfaces.","authors":"Ryo Kato, Keisuke Miyazawa, Takumi Imura, Takeo Minamikawa","doi":"10.1093/bbb/zbae176","DOIUrl":"10.1093/bbb/zbae176","url":null,"abstract":"<p><p>Microbial surfaces play a critical role in various biological processes, including cell adhesion and biofilm formation. Understanding these surfaces at the nanoscale is essential for both fundamental and applied microbiology. This review explores recent advancements in nanoscale structural and chemical analyses of microbial surfaces, with a focus on vibrational spectroscopy, such as Raman spectroscopy, infrared spectroscopy, and atomic force microscopy. The review also discusses current challenges of these techniques, including variability in sample preparation and the reproducibility of data, and highlights future directions in nanoscale analysis that could lead to new insights in microbial physiology, antimicrobial resistance, and biofilm research.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"489-495"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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