携带反刍硒单胞菌质酵素合成酶基因的重组大肠杆菌厌氧产质酵素。

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI:10.1093/bbb/zbae208

Naoki Yoshii, Keita Higuchi, Tomoko Onodera, Naoki Abe, Jun Kaneko

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引用次数: 0

摘要

在厌氧条件下，表达反刍硒单胞菌SrPlsAR的大肠杆菌产生的plasmalogen几乎占总磷脂含量的60%。同时检测到血浆乙醇胺和血浆甘油，sn-1乙烯醚衍生的主要酰基醛为C16:1。Plasmalogen的合成受到atp结合位点和Cys突变的影响，Cys可能参与[4Fe-4S]簇的形成。

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Anaerobic plasmalogen production in recombinant Escherichia coli carrying plasmalogen synthase gene from Selenomonas ruminantium.

Escherichia coli expressing SrPlsAR from Selenomonas ruminantium produces plasmalogen, comprising almost 60% of the total phospholipid content under anaerobic conditions. Both plasmenylethanolamine and plasmenylglycerol were detected, and the major acyl aldehyde derived from sn-1 vinyl ether was C16:1. Plasmalogen synthesis is affected by mutations in ATP-binding sites and Cys is expected to be involved in the formation of the [4Fe-4S] cluster.

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).