发布求助
文献互助 智能选刊 最新文献

Bioscience, Biotechnology, and Biochemistry最新文献

筛选
英文 中文
Secretory expression in Bacillus subtilis, purification and characterization of a persistent protein-degrading enzyme from Nocardiopsis sp. TOA-1.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-10 DOI: 10.1093/bbb/zbae191
Aoto Takano, Mamiko Yano, Tomoka Nakamura, Kazufumi Takano, Shun-Ichi Tanaka
{"title":"Secretory expression in Bacillus subtilis, purification and characterization of a persistent protein-degrading enzyme from Nocardiopsis sp. TOA-1.","authors":"Aoto Takano, Mamiko Yano, Tomoka Nakamura, Kazufumi Takano, Shun-Ichi Tanaka","doi":"10.1093/bbb/zbae191","DOIUrl":"https://doi.org/10.1093/bbb/zbae191","url":null,"abstract":"<p><p>Keratinase from Nocardiopsis sp. TOA-1 (NAPase) holds significant potential for industrial and medical applications. Here, we developed a heterologous secretory expression system for NAPase in Bacillus subtilis. The recombinant enzyme exhibited catalytic properties comparable to the native enzyme, demonstrating its suitability for further protein engineering. This work provides a foundation for enhancing NAPase activity and stability, expediting its biotechnological applications.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Angiogenin-catalyzed cleavage within tRNA anticodon-loops identified by cP-RNA-seq.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-10 DOI: 10.1093/bbb/zbae192
Megumi Shigematsu, Ryuma Matsubara, Justin Gumas, Takuya Kawamura, Yohei Kirino
{"title":"Angiogenin-catalyzed cleavage within tRNA anticodon-loops identified by cP-RNA-seq.","authors":"Megumi Shigematsu, Ryuma Matsubara, Justin Gumas, Takuya Kawamura, Yohei Kirino","doi":"10.1093/bbb/zbae192","DOIUrl":"https://doi.org/10.1093/bbb/zbae192","url":null,"abstract":"<p><p>Angiogenin (Ang), an endoribonuclease belonging to the RNase A superfamily, cleaves the anticodon-loops of tRNAs to produce tRNA half molecules. Although previous studies have demonstrated the involvement of Ang in the pathobiology of neurodegenerative disorders, the characterization of Ang-generated tRNA halves in neuronal cells remains limited. This is partly due to the technical limitations of standard RNA-seq methods, which cannot capture Ang-generated RNAs containing a 2',3'-cyclic phosphate (cP). In this report, we established an Ang treatment model using SH-SY5Y, a human neuroblastoma cell line, and demonstrated Ang-dependent accumulation of tRNA halves. By performing cP-RNA-seq, which selectively captures cP-containing RNAs, we identified Ang-generated tRNA halves and the specific cleavage positions within tRNA anticodon-loops responsible for their generation. Our results provide insights into the anticodon-loop cleavage and the selective production of a specific subset of tRNA halves by Ang.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PDZK1 gene transfer ameliorates lipopolysaccharide-induced cholestasis in rats by rescuing hepatic ABC transporters.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-10 DOI: 10.1093/bbb/zbae190
Tao Wu, Ji Wu, Li Liu, Hongping Song
{"title":"PDZK1 gene transfer ameliorates lipopolysaccharide-induced cholestasis in rats by rescuing hepatic ABC transporters.","authors":"Tao Wu, Ji Wu, Li Liu, Hongping Song","doi":"10.1093/bbb/zbae190","DOIUrl":"https://doi.org/10.1093/bbb/zbae190","url":null,"abstract":"<p><p>Lipopolysaccharide (LPS) causes inflammatory cholestasis in sepsis. We investigated the role of PDZK1 in the repression of ABC transporters in LPS-induced cholestasis. Lentiviral gene transfer of PDZK1 to rats was conducted to explore its influence on cholestasis induced by LPS. And the effect of lentivirus-mediated shRNA targeting PDZK1 on ABC transporters in rat liver BRL-3A cells was evaluated. Lentiviral vector encoding rat PDZK1 was administered to rats by tail intravenous injection. Obviously elevated serum total bile acid (TBA) level and liver biochemical markers in cholestatic rats were decreased by the Lv-PDZK1 delivery. Also Lv-PDZK1 delivery stimulated the suppressed hepatic ABC transporters expression. In vitro, after the lentiviral vector LV3/PDZK1 shRNA transfection, no expression of PDZK1 and mild expression of ABC transporters were detected in BRL-3A cells by Western blotting. Our results indicate that the lentiviral-mediated hepatocyte PDZK1 expression ameliorates LPS-induced cholestasis in rats by rescuing hepatic ABC transporters expression.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nitrogen deficiency influences strigolactone levels in basal parts of shoots and shoot branching phenotype in Arabidopsis thaliana.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-09 DOI: 10.1093/bbb/zbae185
Riko Sakioka, Kaori Yoneyama
{"title":"Nitrogen deficiency influences strigolactone levels in basal parts of shoots and shoot branching phenotype in Arabidopsis thaliana.","authors":"Riko Sakioka, Kaori Yoneyama","doi":"10.1093/bbb/zbae185","DOIUrl":"https://doi.org/10.1093/bbb/zbae185","url":null,"abstract":"<p><p>Strigolactones (SLs) are allelochemicals attracting both symbiotic arbuscular mycorrhizal (AM) fungi and root parasitic plants and a novel class of phytohormones inhibiting lateral shoot branching. In general, nutrient deficiency significantly promotes SL production in roots and exudation into the rhizosphere, and also induces suppressed shoot branching phenotype in host plants of AM fungi including rice, tomato, maize, etc. Nutrient deficiencies also inhibit the shoot branching in Arabidopsis thaliana (hereafter Arabidopsis), a non-host of AM fungi, while the level of carlactone, a non-canonical SL and the SL precursor for the other SLs, was reported to be unaffected. Because Arabidopsis SLs including CL and methyl carlactonoate,, are highly unstable, relationships between SL levels and shoot branching in Arabidopsis remain elusive. Herein, we demonstrate that nitrogen deficiency increases SL levels in the basal part of shoots in Arabidopsis and lateral shoot branching appears to be strongly regulated by these SLs.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Moonlight function of antioxidants.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-09 DOI: 10.1093/bbb/zbae186
Masanori Itakura, Kosuke Yamaguchi, Koji Uchida
{"title":"Moonlight function of antioxidants.","authors":"Masanori Itakura, Kosuke Yamaguchi, Koji Uchida","doi":"10.1093/bbb/zbae186","DOIUrl":"https://doi.org/10.1093/bbb/zbae186","url":null,"abstract":"<p><p>We take a wide variety of antioxidants, including polyphenols, daily from our diet. They are generally considered to be beneficial for our health. However, the intrinsic function of antioxidants in biological systems remained unknown. On the other hand, antioxidants in general are sensitive to oxidation, generating their oxidized intermediates. Intriguingly, these intermediates are highly reactive to proteins. Although the specific cellular targets and response mechanism remain unclear, protein modification by oxidized antioxidants may represent the intrinsic \"moonlight\" function of antioxidants by taking on a secondary role beyond their traditional activity. This mini-review summarizes recent findings on antioxidants, with a particular focus on the interactions of antioxidant-modified proteins with histones.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isomaltodextrin inhibits kidney enlargement induced by a high-protein diet through its metabolism by gut microbiota.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-06 DOI: 10.1093/bbb/zbae188
Ryodai Takagaki, Jun Takahashi, Shin Endo, Ryota Kujirai, Mizuki Abe, Koichi Kikuchi, Chitose Suzuki, Yotaro Matsumoto, Yoshihisa Tomioka, Takaaki Abe, Hidetoshi Morita
{"title":"Isomaltodextrin inhibits kidney enlargement induced by a high-protein diet through its metabolism by gut microbiota.","authors":"Ryodai Takagaki, Jun Takahashi, Shin Endo, Ryota Kujirai, Mizuki Abe, Koichi Kikuchi, Chitose Suzuki, Yotaro Matsumoto, Yoshihisa Tomioka, Takaaki Abe, Hidetoshi Morita","doi":"10.1093/bbb/zbae188","DOIUrl":"https://doi.org/10.1093/bbb/zbae188","url":null,"abstract":"<p><p>To evaluate the effects of the soluble fiber isomaltodextrin in a protein-biased diet, a 21-day protein diet trial was conducted in rats, with 60% of the calories derived from protein. The results revealed that the high-protein diet alone led to a significant increase in kidney weight. In contrast, the consumption of water with 5% isomaltodextrin dissolved in it, along with a high-protein diet, suppressed this weight gain. To elucidate this mechanism, an analysis of serum urea toxins confirmed that the concentrations of phenyl sulfate were significantly higher with high protein, and significantly lower with isomaltodextrin. The impact of a high-protein on increased phenol in cecal contents, an increased that was mitigated by the isomaltodextrin. This suggests that the inhibitory effect of isomaltodextrin on renal hypertrophy was due to the suppression of urea toxin precursor production by the gut microbiota.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new phthalide derivative from the mushroom Cyclocybe cf. erebia culture filtrate affects the phase of circadian rhythms in mouse fibroblasts. 一种来自蘑菇 Cyclocybe cf. erebia 培养滤液的新邻苯二甲酸酯衍生物会影响小鼠成纤维细胞的昼夜节律相位。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-05 DOI: 10.1093/bbb/zbae187
Yusei Kobayashi, Yuanyuan Lu, Nan Li, Naoki Endo, Kozue Sotome, Kotomi Ueno, Yu Tahara, Atsushi Ishihara
{"title":"A new phthalide derivative from the mushroom Cyclocybe cf. erebia culture filtrate affects the phase of circadian rhythms in mouse fibroblasts.","authors":"Yusei Kobayashi, Yuanyuan Lu, Nan Li, Naoki Endo, Kozue Sotome, Kotomi Ueno, Yu Tahara, Atsushi Ishihara","doi":"10.1093/bbb/zbae187","DOIUrl":"https://doi.org/10.1093/bbb/zbae187","url":null,"abstract":"<p><p>Circadian rhythms are biological systems that provide approximately 24-hour cycles for the behavior and physiological functions of organisms. As diverse modern lifestyles often cause disturbances in circadian rhythms, new approaches to their regulation are required. Therefore, new compounds that affect circadian rhythms have been explored in edible mushrooms. The extract from the culture filtrate of Cyclocybe cf. erebia showed activity that advanced the circadian rhythm in a bioassay with mouse fibroblasts expressing the LUCIFERASE protein under the control of the Period2 promoter. Bioassay-guided fractionation of the extract resulted in the isolation of the compound. Spectroscopic analyses identified the compound as a phthalide derivative, and the compound was named cyclocybelide. Treatment of mouse fibroblasts with the compound shifted the circadian rhythm forward, irrespective of the timing of treatment. In addition, some phthalide derivatives with hydroxy and methoxy groups showed similar effects on circadian rhythms.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative analysis of sterol balance in a mouse model of hepatic lipid accumulation induced by cholesterol and cholic acid supplementation.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-04 DOI: 10.1093/bbb/zbae183
Hinata Soma, Ryo Yoshida, Satoshi Ishizuka
{"title":"Quantitative analysis of sterol balance in a mouse model of hepatic lipid accumulation induced by cholesterol and cholic acid supplementation.","authors":"Hinata Soma, Ryo Yoshida, Satoshi Ishizuka","doi":"10.1093/bbb/zbae183","DOIUrl":"https://doi.org/10.1093/bbb/zbae183","url":null,"abstract":"<p><p>The cholesterol balance and bile acid metabolism in a mouse model of hepatic lipid accumulation induced by a diet supplemented with cholesterol and cholic acid (CA) were quantitatively evaluated. The mice were fed diets supplemented with different levels of cholesterol (0, 3, or 6 g/kg diet) and CA (0.5 g/kg diet) for six weeks. Cholesterol supplementation doubled the hepatic triglyceride concentration, regardless of the supplementation level, without inflammation or gallstone formation. Both cholesterol supplementations enhanced fecal excretion of muricholic acid. Additionally, the higher cholesterol supplementation led to an increase in fecal cholesterol excretion, accompanied by elevated expression of hepatic cholesterol exporters and a reduction in fecal bile acid excretion. In this mouse study, supplementation with 3 g cholesterol/kg diet and 0.5 g CA/kg diet was sufficient to induce hepatic lipid accumulation.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heterologous expression and enzymological characterization of L-glutamate oxidase from the marine actinomycete Streptomyces lydicamycinicus NBRC 110027.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-04 DOI: 10.1093/bbb/zbae184
Tadao Oikawa, Kazuya Yamanaka
{"title":"Heterologous expression and enzymological characterization of L-glutamate oxidase from the marine actinomycete Streptomyces lydicamycinicus NBRC 110027.","authors":"Tadao Oikawa, Kazuya Yamanaka","doi":"10.1093/bbb/zbae184","DOIUrl":"https://doi.org/10.1093/bbb/zbae184","url":null,"abstract":"<p><p>We successfully constructed a heterologous expression system for L-glutamate oxidase from the marine actinomycete Streptomyces lydicamycinicus NBRC 110027 (Sl-LGOX) in Escherichia coli BL21(DE3) as a host. This is the first example of L-glutamate oxidase from a marine microorganism. A chemically synthesized gene optimized for codon usage in E. coli was used as the inserted fragment, which was effective for enzyme expression. We expressed Sl-LGOX in the soluble fraction of E. coli BL21(DE3)/pET21b-Sl-lgox. We also succeeded in purifying recombinant Sl-LGOX (rSl-LGOX) to homogeneity from the cell-free extract of this clone via an Ni-NTA column. rSl-LGOX showed high specificity for L-Glu and was active and stable over a wide range of temperatures and pH values. In particular, it showed high specific activity and stability at an acidic pH. A variety of applications can take advantage of the unique enzymatic properties of rSl-LGOX.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
This review was written in response to the author's receipt of the JSBBA Award for Young Scientists in 2024.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-11-28 DOI: 10.1093/bbb/zbae182
Haruya Takahashi
{"title":"This review was written in response to the author's receipt of the JSBBA Award for Young Scientists in 2024.","authors":"Haruya Takahashi","doi":"10.1093/bbb/zbae182","DOIUrl":"https://doi.org/10.1093/bbb/zbae182","url":null,"abstract":"<p><p>Proper diet is crucial for obesity prevention. Food health research primarily focuses on two aspects: the pathogenesis of lifestyle-related diseases caused by obesity and the identification of dietary components that can aid in the prevention and treatment of lifestyle-related diseases. Substantial knowledge has been accumulated regarding these aspects via health function evaluations based on biological experiments. However, the intricate causal relationships and specific biological metabolites or food compounds that affect health remain unclear. To address these issues, recent my studies have incorporated mass spectrometry data, particularly metabolomic data obtained via comprehensive component analysis, to enhance health function evaluation via biological experiments. These studies have revealed various biological and healthy compounds derived from different food materials for the management of obesity-induced metabolic disorders.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信