Bioscience, Biotechnology, and Biochemistry最新文献_第2页

Generation of citric acid-hyperproducers independent of methanol effect by high-level expression of cexA encoding citrate exporter in Aspergillus tubingensis. 通过在管曲霉中高水平表达编码柠檬酸盐输出器的 cexA，产生独立于甲醇效应的柠檬酸高产菌。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI: 10.1093/bbb/zbae099

Isato Yoshioka, Kohtaro Kirimura

引用次数: 0

Evaluation of antifungal activity of cerium oxide nanoparticles and associated cellular responses. 评估氧化铈纳米粒子的抗真菌活性及相关细胞反应。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI: 10.1093/bbb/zbae101

Shunsuke Nishino, Sayoko Oiki, Yoshimasa Yamana, Daisuke Hagiwara

{"title":"Evaluation of antifungal activity of cerium oxide nanoparticles and associated cellular responses.","authors":"Shunsuke Nishino, Sayoko Oiki, Yoshimasa Yamana, Daisuke Hagiwara","doi":"10.1093/bbb/zbae101","DOIUrl":"10.1093/bbb/zbae101","url":null,"abstract":"Cerium oxide (CeO2) nanoparticles, as a metal oxide nanomaterial, are increasingly used for various industrial and biomedical applications. Although their cytotoxicity to bacteria and the associated mechanisms have attracted particular attention, the mechanisms behind their antifungal effects have remained unclear. This study investigated the antifungal properties of CeO2, focusing on Aspergillus oryzae. CeO2 inhibited fungal spore germination on solid substrates, and the effect was fungistatic rather than fungicidal. CeO2 inhibited fungal growth, especially under UV irradiation, and induced reactive oxygen species (ROS) production. Tocopherol reduced the intracellular ROS levels and the growth-inhibitory effects of CeO2, suggesting that ROS are involved in these growth-inhibitory effects. Transcriptomic analysis revealed upregulated expression of genes related to phospholipases and phosphate metabolism. CeO2 affected phosphate ion concentration in the medium, potentially influencing cellular responses. This research provided valuable insights into the antifungal effects of CeO2 application, which differ from those of conventional photocatalysts like TiO2.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of an engineered salt-inducible proline biosynthetic operon in a glutamic acid over-producing mutant, Halomonas elongata GOP, confers increased proline yield due to enhanced growth under high-salinity conditions. 在谷氨酸过量产生突变体 Halomonas elongata GOP 中表达经过设计的盐诱导脯氨酸生物合成操作子，可在高盐度条件下提高生长速度，从而增加脯氨酸产量。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI: 10.1093/bbb/zbae102

Huynh Cong Khanh, Pulla Kaothien-Nakayama, Ziyan Zou, Hideki Nakayama

引用次数: 0

Anti-inflammatory effect of covalent PPARγ ligands that have a hybrid structure of GW9662 and a food-derived cinnamic acid derivative. 具有 GW9662 和食品来源肉桂酸衍生物混合结构的共价 PPARγ 配体的抗炎作用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI: 10.1093/bbb/zbae094

Shinano Miyazawa, Misa Sakai, Yuma Omae, Yusuke Ogawa, Hideyuki Shigemori, Yusaku Miyamae

{"title":"Anti-inflammatory effect of covalent PPARγ ligands that have a hybrid structure of GW9662 and a food-derived cinnamic acid derivative.","authors":"Shinano Miyazawa, Misa Sakai, Yuma Omae, Yusuke Ogawa, Hideyuki Shigemori, Yusaku Miyamae","doi":"10.1093/bbb/zbae094","DOIUrl":"10.1093/bbb/zbae094","url":null,"abstract":"Peroxisome proliferator-activated receptor γ (PPARγ) belongs to the nuclear receptor superfamily and is involved in the inflammatory process. Previously, we synthesized the ligands of PPARγ that possess the hybrid structure of a food-derived cinnamic acid derivative (CA) and GW9662, an irreversible PPARγ antagonist. These ligands activate the transcription of PPARγ through the covalent bond formation with the Cys285 residue of PPARγ, whereas their anti-inflammatory effect has not been examined yet. Here, we show the anti-inflammatory effect of the covalent PPARγ ligands in RAW264 cells, murine macrophage-like cells. GW9662 suppressed the production of nitric oxide (NO) stimulated by lipopolysaccharide and exerted a synergistic effect in combination with CA. The compounds bearing their hybrid structure dramatically inhibited NO production and transcription of proinflammatory cytokines. A comparison study suggested that the 2-chloro-5-nitrobenzoyl group of the ligands is important for anti-inflammation. Furthermore, we synthesized an alkyne-tagged analogue that becomes an activity-based probe for future mechanistic study.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141466150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of an antimalarial compound, burnettiene A, with a multidrug-sensitive Saccharomyces cerevisiae screening system based on mitochondrial function inhibitory activity. 利用基于线粒体功能抑制活性的多药敏感酿酒酵母筛选系统发现抗疟疾化合物 burnettiene A。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI: 10.1093/bbb/zbae098

Aoi Kimishima, Atsuka Nishitomi, Iori Tsuruoka, Katsuyuki Sakai, Rei Hokari, Masako Honsho, Sota Honma, Yuki Ono, Naozumi Kondo, Hayama Tsutsumi, Yuta Kikuchi, Toshiyuki Tokiwa, Hiroki Kojima, Mayuka Higo, Kenichi Nonaka, Yuki Inahashi, Masato Iwatsuki, Shin-Ichi Fuji, Jun-Pil Jang, Jae-Hyuk Jang, Takumi Chinen, Takeo Usui, Yukihiro Asami

{"title":"Discovery of an antimalarial compound, burnettiene A, with a multidrug-sensitive Saccharomyces cerevisiae screening system based on mitochondrial function inhibitory activity.","authors":"Aoi Kimishima, Atsuka Nishitomi, Iori Tsuruoka, Katsuyuki Sakai, Rei Hokari, Masako Honsho, Sota Honma, Yuki Ono, Naozumi Kondo, Hayama Tsutsumi, Yuta Kikuchi, Toshiyuki Tokiwa, Hiroki Kojima, Mayuka Higo, Kenichi Nonaka, Yuki Inahashi, Masato Iwatsuki, Shin-Ichi Fuji, Jun-Pil Jang, Jae-Hyuk Jang, Takumi Chinen, Takeo Usui, Yukihiro Asami","doi":"10.1093/bbb/zbae098","DOIUrl":"10.1093/bbb/zbae098","url":null,"abstract":"In this paper, we describe our discovery of burnettiene A (1) as an antimalarial compound from the culture broth of Lecanicillium primulinum (current name: Flavocillium primulinum) FKI-6715 strain utilizing our original multidrug-sensitive yeast system. This polyene-decalin polyketide natural product was originally isolated as an antifungal active compound from Aspergillus burnettii. However, the antifungal activity of 1 has been revealed in only one fungal species, and the mechanism of action of 1 remains unknown. After the validation of mitochondrial function inhibitory of 1, we envisioned a new antimalarial drug discovery platform based on mitochondrial function inhibitory activity. We evaluated antimalarial activity and 1 showed antimalarial activity against Plasmodium falciparum FCR3 (chloroquine sensitive) and the K1 strain (chloroquine resistant). Our study revealed the utility of our original screening system based on a multidrug-sensitive yeast and mitochondrial function inhibitory activity for the discovery of new antimalarial drug candidates.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cocoa extract induces browning of white adipocytes and improves glucose intolerance in mice fed a high-fat diet. 可可提取物可诱导白色脂肪细胞棕色化，并改善高脂饮食小鼠的葡萄糖不耐受症。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI: 10.1093/bbb/zbae105

Eito Yonemoto, Risa Ihara, Emi Tanaka, Takakazu Mitani

引用次数: 0

Isolation of microorganisms from the feces of Kitasato Yakumo beef cattle as bioactive natural product producers. 从北里八云肉牛粪便中分离可产生生物活性天然产品的微生物。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-20 DOI: 10.1093/bbb/zbae103

Aoi Kimishima, Atsuka Nishitomi, Naozumi Kondo, Sota Honma, Masako Honsho, Sota Negami, Serino Maruyama, Kazuki Taguchi, Hidehito Matsui, Hideaki Hanaki, Takumi Chinen, Takeo Usui, Hideki Ogasawara, Yukihiro Asami

引用次数: 0

Synergistic xylan decomposition by a reducing-end xylose-releasing exo-oligoxylanase with other xylanolytic enzymes derived from Paenibacillus xylaniclasticus strain TW1. 一种还原端木糖释放外低聚木糖酶与其他木糖分解酶协同分解木糖，这些酶来自木糖胶冻芽孢杆菌（Paenibacillus xylaniclasticus）菌株 TW1。

IF 1.6 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-18 DOI: 10.1093/bbb/zbae130

Koki Taniguchi,Shuichi Karita,Midori Umekawa

引用次数: 0

β-Caryophyllene, a dietary phytocannabinoid, alleviates high-fat diet-induced hepatic steatosis in mice via AMPK activation. 膳食植物大麻素β-茶碱通过激活AMPK缓解高脂饮食诱发的小鼠肝脂肪变性。

IF 1.6 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-13 DOI: 10.1093/bbb/zbae129

Ryosuke Kamikubo,Hiroki Yoshida,Taiki Fushimi,Yuki Kamei,Mitsugu Akagawa

{"title":"β-Caryophyllene, a dietary phytocannabinoid, alleviates high-fat diet-induced hepatic steatosis in mice via AMPK activation.","authors":"Ryosuke Kamikubo,Hiroki Yoshida,Taiki Fushimi,Yuki Kamei,Mitsugu Akagawa","doi":"10.1093/bbb/zbae129","DOIUrl":"https://doi.org/10.1093/bbb/zbae129","url":null,"abstract":"β-Caryophyllene (BCP), a dietary phytocannabinoid, significantly suppresses palmitate-induced lipid accumulation in human HepG2 hepatocytes via activation of AMP-activated protein kinase (AMPK) signaling. The objective of the preset research was to assess whether oral administration of BCP alleviates obesity-induced hepatic steatosis in mice through AMPK activation. We examined the protective action of supplementation of 0.3% BCP (w/w) in a high-fat diet (HFD) on C57BL/6 J mice for 12 weeks. BCP supplementation evidently ameliorated histological hepatic steatosis features, and significantly reduced triglycerides and cholesterol levels in liver, and serum levels of aspartate aminotransferase and alanine aminotransferase as compared with non-supplemented HFD-fed mice. Immunoblotting revealed that BCP supplementation in HFD-fed mice also caused hepatic AMPK activation. Furthermore, treatment with BCP in HFD-fed mice significantly suppressed body weight gain and attenuated obesity-related phenotypes relative to the HFD mice. Our results suggest the usefulness of BCP in the prevention of obesity-related liver steatosis and liver injury.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142268566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aurantiochytrium mutant strains exhibiting different colony colors altered the contents of squalene. 表现出不同菌落颜色的 Aurantiochytrium 突变菌株改变了角鲨烯的含量。

IF 1.6 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-13 DOI: 10.1093/bbb/zbae128

Tianjing Yang,Shohei Kubo,Darryl Joy Juntila,Yasushi Iwata,Kanako Tomita,Yoshihiro Hase,Yutaka Oono,Hiroya Araie,Kohei Yoneda,Yoshiaki Maeda,Iwane Suzuki

引用次数: 0