Bioscience, Biotechnology, and Biochemistry最新文献_第2页

Marine bacterium Cobetia sp. strain GM produces fucose-rich extracellular polymeric substance. 海洋细菌Cobetia sp.菌株GM产生富聚焦的胞外聚合物质。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf025

Joe Watanabe, Kenjiro Sugiyama, Katsuhiko Fujii

引用次数: 0

Suppressive Effect of Transient Receptor Potential Vanilloid 1 on Olfactory Receptor Signal Transduction. 瞬时受体电位香兰素1对嗅觉受体信号转导的抑制作用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf093

Sakura Moriyama, Shuji Hinuma, Shun'ichi Kuroda

{"title":"Suppressive Effect of Transient Receptor Potential Vanilloid 1 on Olfactory Receptor Signal Transduction.","authors":"Sakura Moriyama, Shuji Hinuma, Shun'ichi Kuroda","doi":"10.1093/bbb/zbaf093","DOIUrl":"https://doi.org/10.1093/bbb/zbaf093","url":null,"abstract":"We investigated the effect of transient receptor potential vanilloid 1 (TRPV1) on ligand-stimulated olfactory receptor (OR) activation (intracellular cAMP elevation). The co-expression of TRPV1 with OR51E1 in HEK293T cells suppressed the activation of OR51E1 stimulated with isovaleric acid. This suppression was further potentiated by capsaicin stimulation. On the other hand, capsaicin suppressed the ligand-induced cAMP production in HEK293T cells expressing OR51E1 without co-transfection of the TRPV1 expression plasmid. Treatment with siRNA for TRPV1 offset this capsaicin's effect on ligand-induced OR51E1 activation, suggesting the involvement of endogenously expressed TRPV1 in this suppression. Additionally, TRPV1-dependent suppression of OR51E1 activation was abolished by Ca2+ depletion in the extracellular fluid. A calcium ionophore (A23187) exhibited effects like TRPV1 on the OR response. Furthermore, the suppressive effect of A23187 was inhibited by a G protein-coupled receptor kinase (GRK) inhibitor CCG21022. Our results indicate that TRPV1 suppresses OR activation via Ca²⁺ influx, followed by GRK activation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agrose matrix stiffness mediated neuroprotective effects against H2O2-induced oxidative stress in HT22 spheroids. 脂糖基质硬度介导HT22球体对h2o2诱导的氧化应激的神经保护作用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf094

Guang-Zhen Jin

引用次数: 0

Biochemical and structural analysis of the mechanism for the catalysis and specificity of cellobiose 2-epimerase from Rhodothermus marinus. 海红藻纤维二糖2-外甲酰基酶的催化机制及特异性的生化和结构分析。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf042

Wataru Saburi, Hirohiko Muto-Fukiya, Nongluck Jaito, Koji Kato, Jian Yu, Min Yao, Haruhide Mori

{"title":"Biochemical and structural analysis of the mechanism for the catalysis and specificity of cellobiose 2-epimerase from Rhodothermus marinus.","authors":"Wataru Saburi, Hirohiko Muto-Fukiya, Nongluck Jaito, Koji Kato, Jian Yu, Min Yao, Haruhide Mori","doi":"10.1093/bbb/zbaf042","DOIUrl":"10.1093/bbb/zbaf042","url":null,"abstract":"Cellobiose 2-epimerase (CE) catalyzes C-2 epimerization of reducing end d-glucose/d-mannose residue of β-(1→4)-disaccharides, and also slightly catalyzes aldose-ketose conversion. In this study, we investigated the structure-function relationship of Rhodothermus marinus CE (RmCEs). In 2H2O, 2H replaced the 2-H of the reducing end sugar residue, suggesting a proton abstraction-addition mechanism via the cis-enediolate intermediate. The structure of the RmCE-mannobiitol complex showed that His259 was suitable for abstracting 2-H from d-mannose residue, whereas His390 was suitable for the d-glucose residue. H259A and H390A mutations abolished activity for Galβ1-4Man and Galβ1-4Glc formation from Galβ1-4Fru, respectively, and these mutants catalyzed both epimerization and isomerization to Galβ1-4Glc and Galβ1-4Man, respectively. Ala substitution of the residues interacting with the 2-O of the reducing end sugar residue significantly reduced the velocity for epimerization, but not for isomerization. Trp385, stacked onto the non-reducing-end sugar residues of disaccharides, was shown to be important for disaccharide specificity.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"973-984"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the bread containing resistant starch from wheat (Triticum aestivum) on the improvement in bowel movements: a randomized, double-blind, placebo-controlled, crossover study. 含有小麦抗性淀粉（Triticum aestivum）的面包对改善肠道运动的影响：一项随机、双盲、安慰剂对照的交叉研究。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf044

Yosuke Kikuchi, Yuto Otomo, Kazuki Toyota, Satoshi Noma, Shukuko Ebihara

{"title":"Effects of the bread containing resistant starch from wheat (Triticum aestivum) on the improvement in bowel movements: a randomized, double-blind, placebo-controlled, crossover study.","authors":"Yosuke Kikuchi, Yuto Otomo, Kazuki Toyota, Satoshi Noma, Shukuko Ebihara","doi":"10.1093/bbb/zbaf044","DOIUrl":"10.1093/bbb/zbaf044","url":null,"abstract":"This study aimed to evaluate the gut-regulating effects of wheat-derived resistant starch. A randomized, double-blind, placebo-controlled crossover study was conducted with 46 healthy Japanese adults. They were divided into 2 groups, consuming 3.26 g/day of wheat-derived resistant starch and a control food for 4 weeks each, separated by a 4-week washout period. There were no significant differences observed in the primary endpoint of defecation frequency. However, subgroup analysis showed significant improvements in bowel movements for participants who felt a strong sensation of incomplete evacuation. No gut-regulating effects were noted in other subgroups and secondary endpoints. The intake of 3.26 g/day of resistant starch was safe with no overall efficacy observed. Increased stool frequency was observed in participants with less frequent bowel movements and a strong feeling of incomplete evacuation, but no effects were confirmed in other subgroup analyses. No significant changes were observed at secondary endpoints (UMIN ID: UMIN000052543).","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1015-1023"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of a monomeric flavin-containing opine dehydrogenase from bacteria. 从细菌中发现单体含黄素的阿片脱氢酶。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf096

Seiya Watanabe, Kentaroh Yoshiwara, Mizue Date, Yasunori Watanabe

引用次数: 0

Regio-specific synthesis of flavonoid glucuronides using plant UDP-glucuronosyltransferase expressed in yeast. 利用酵母中表达的植物udp -葡萄糖醛基转移酶合成黄酮类葡萄糖醛酸酯的区域特异性研究。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf049

Mst Julia Sultana, Takuto Kurakawa, Miyu Nishikawa, Shinichi Ikushiro

{"title":"Regio-specific synthesis of flavonoid glucuronides using plant UDP-glucuronosyltransferase expressed in yeast.","authors":"Mst Julia Sultana, Takuto Kurakawa, Miyu Nishikawa, Shinichi Ikushiro","doi":"10.1093/bbb/zbaf049","DOIUrl":"10.1093/bbb/zbaf049","url":null,"abstract":"Glucuronidation is a well-established biotransformation process that modifies the physiological and pharmacological properties of small molecules, making it a valuable tool for enhancing the chemical diversity of natural compounds in drug development. However, the chemical synthesis of glucuronides is often complex, time-consuming, and environmentally unsustainable. To overcome these challenges, plant uridine diphosphate (UDP)-glucuronosyltransferase (UGT)-mediated glucuronidation, using transformed yeast, offers a selective and efficient alternative for producing flavonoid glucuronides. This study aimed to conjugate quercetin with glucuronic acid by stably co-transforming Saccharomyces cerevisiae with plant UGTs (UGT78A11 and UGT88D7) and rat UDP-glucose-6-dehydrogenease. The UGT78A11 and UGT88D7 selectively conjugated quercetin at specific positions, producing quercetin-3-O-glucuronide and quercetin-7-O-glucuronide, respectively. The whole-cell biotransformation platform effectively leverages the regio-selectivity of UGT78A11 and UGT88D7 to convert polyhydroxy secondary metabolites into monoglucuronides with promising yields, thereby enhancing the availability and physiological potential of these glucuronides.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"954-964"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of the microalga Pavlova sp. OPMS 30543 with diclofenac attenuates hyperalgesia in adjuvant-induced arthritis mice. 微藻Pavlova sp. OPMS 30543与双氯芬酸联合使用可减轻佐剂性关节炎小鼠的痛症过敏。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf047

Wataru Nemoto, Kotaro Yamada, Ruka Kobayashi, Tomohiro Hoshi, Ken Nakahara, Akihiko Kanamoto, Osamu Nakagawasai, Koichi Tan-No

引用次数: 0

Downsizing effect on direct electron transfer-type bioelectrocatalysis by d-fructose dehydrogenase with structural insight. d-果糖脱氢酶对直接电子转移型生物电催化的缩小效应及其结构研究。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf043

Yohei Suzuki, Yuki Kitazumi, Osamu Shirai, Keisei Sowa

引用次数: 0

Intracellular electron donor stripping to identify electron donors of indigo reduction. 胞内电子给体剥离鉴定靛蓝还原的电子给体。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf054

Hiroki Tanaka, Mayu Kikuchi, Kenji Kano, Eiji Sakuradani, Jun Ogawa, Kouichi Kuroda, Michiki Takeuchi

引用次数: 0