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Downsizing Effect on Direct Electron Transfer-Type Bioelectrocatalysis by d-Fructose Dehydrogenase with Structural Insight.
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-26 DOI: 10.1093/bbb/zbaf043
Yohei Suzuki, Yuki Kitazumi, Osamu Shirai, Keisei Sowa
{"title":"Downsizing Effect on Direct Electron Transfer-Type Bioelectrocatalysis by d-Fructose Dehydrogenase with Structural Insight.","authors":"Yohei Suzuki, Yuki Kitazumi, Osamu Shirai, Keisei Sowa","doi":"10.1093/bbb/zbaf043","DOIUrl":"https://doi.org/10.1093/bbb/zbaf043","url":null,"abstract":"<p><p>Membrane-bound heterotrimeric d-fructose dehydrogenase (FDH) from Gluconobacter japonicus exhibits distinct direct electron transfer (DET)-type bioelectrocatalytic activity. FDH contains three heme c moieties (heme 1c, 2c, and 3c), and the two downsized variants lacking heme 1c moiety (Δ1c FDH) or heme 1c and 2c moieties (Δ1c2c FDH) were constructed in our previous research. Recently, we elucidated the entire structure of FDH, enabling a structural perspective in mechanistic discussions. In this study, we quantitatively analyzed the downsizing effect of Δ1c- and Δ1c2c-deletion on DET-type bioelectrocatalysis. Non-catalytic redox signals of adsorbed enzymes were successfully obtained, providing a basis for independently evaluating kinetic parameters from DET-type catalytic waves. Analytical results revealed that the downsizing enhanced the electron transfer rate constant by 7-fold and 4-fold for Δ1c FDH and Δ1c2c FDH, respectively. Furthermore, the acceleration factors were discussed using structural predictions of the variants.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PNGase activity and free N-glycans in phloem fluid prepared from Nerium oleander (oleander tree).
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-25 DOI: 10.1093/bbb/zbaf038
Fuki Otaguro, Yoshinobu Kimura, Megumi Maeda
{"title":"PNGase activity and free N-glycans in phloem fluid prepared from Nerium oleander (oleander tree).","authors":"Fuki Otaguro, Yoshinobu Kimura, Megumi Maeda","doi":"10.1093/bbb/zbaf038","DOIUrl":"https://doi.org/10.1093/bbb/zbaf038","url":null,"abstract":"<p><p>Free N-glycans (FNGs) occur ubiquitously in growing plants. Recently, it was reported that these FNGs interact with auxin. In this study, we investigated whether PNGase activity responsible to produce the FNGs occurs in the extracellular fluid, where auxin is present during its polar transfer. Here, we report the occurrences of PNGase activity and FNGs in the phloem fluid.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oral administration of astaxanthin mitigates chronological skin aging in mice. 口服虾青素可减轻小鼠的皮肤老化。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae205
Shuyu Liu, Yuki Manabe, Tatsuya Sugawara
{"title":"Oral administration of astaxanthin mitigates chronological skin aging in mice.","authors":"Shuyu Liu, Yuki Manabe, Tatsuya Sugawara","doi":"10.1093/bbb/zbae205","DOIUrl":"10.1093/bbb/zbae205","url":null,"abstract":"<p><p>Intrinsic skin aging is a chronological decline in skin texture and function influenced largely by genetic factors. Aged skin exhibits morphological alterations, including wrinkling, dryness, and roughness, along with dysfunctional changes in the skin barrier. In this study, the in vivo anti-intrinsic aging efficacy of dietary astaxanthin extracted from Haematococcus pluvialis on the skin was evaluated using aged C57BL/6 J mice. As a result, dietary supplementation of 0.1% astaxanthin significantly alleviated the defects in skin's water retention capacity, viscoelasticity, and reduced wrinkle formation induced by intrinsic aging. Furthermore, gene expression analysis revealed that dietary astaxanthin was capable of mediating genes related to the proliferation and differentiation of skin cells, degradation of proteins in the extracellular matrix and dermal-epidermal junction, synthesis of natural moisturizing factors, and maintenance of skin barrier function. Together, our data indicate that dietary astaxanthin has potential applications as a novel ingredient in nutricosmetics against chronological skin aging.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"612-621"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Co-culture of Aspergillus niger IFM 59706 and RAW264 cells enhances the production of aurasperone A with nitric oxide inhibitory activity. 黑曲霉IFM 59706细胞与RAW264细胞共培养可提高aurasperone A的产量，并具有NO抑制活性。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae211
Yukiko Ujie, Shun Saito, Tomoya Banno, Takashi Yaguchi, Midori A Arai
{"title":"Co-culture of Aspergillus niger IFM 59706 and RAW264 cells enhances the production of aurasperone A with nitric oxide inhibitory activity.","authors":"Yukiko Ujie, Shun Saito, Tomoya Banno, Takashi Yaguchi, Midori A Arai","doi":"10.1093/bbb/zbae211","DOIUrl":"10.1093/bbb/zbae211","url":null,"abstract":"<p><p>Most actinomycetes and fungi have a multitude of silent biosynthetic genes whose activation could lead to the production of new natural products. Our group recently designed and used a co-culture method to isolate new natural products, based on the idea that pathogens might produce immune suppressors to avoid attack by immune cells. Here, we searched for compounds produced by the co-culture of immune cells with pathogenic fungi isolated from clinical specimens. The production of dimeric naphtho-γ-pyrone aurasperone A (1) was enhanced by the co-culture of pathogenic fungus Aspergillus niger IFM 59706 and RAW264 mouse macrophage-like cells. The absolute configuration of 1 was confirmed by comparison with the reported electronic circular dichroism spectrum. This is the first report of the inhibitory activity of 1 on nitric oxide production, an inflammatory mediator.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"541-547"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anaerobic plasmalogen production in recombinant Escherichia coli carrying plasmalogen synthase gene from Selenomonas ruminantium. 携带反刍硒单胞菌质酵素合成酶基因的重组大肠杆菌厌氧产质酵素。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae208
Naoki Yoshii, Keita Higuchi, Tomoko Onodera, Naoki Abe, Jun Kaneko
{"title":"Anaerobic plasmalogen production in recombinant Escherichia coli carrying plasmalogen synthase gene from Selenomonas ruminantium.","authors":"Naoki Yoshii, Keita Higuchi, Tomoko Onodera, Naoki Abe, Jun Kaneko","doi":"10.1093/bbb/zbae208","DOIUrl":"10.1093/bbb/zbae208","url":null,"abstract":"<p><p>Escherichia coli expressing SrPlsAR from Selenomonas ruminantium produces plasmalogen, comprising almost 60% of the total phospholipid content under anaerobic conditions. Both plasmenylethanolamine and plasmenylglycerol were detected, and the major acyl aldehyde derived from sn-1 vinyl ether was C16:1. Plasmalogen synthesis is affected by mutations in ATP-binding sites and Cys is expected to be involved in the formation of the [4Fe-4S] cluster.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"594-598"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of microemulsion system on water dispersibility and bioavailability of γ-oryzanol. 微乳液体系对γ-谷维醇水分散性和生物利用度的影响。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbaf002
Junya Ito, Naoko Kumagai, Ayaka Suzuki, Naoki Shoji, Isabella Supardi Parida, Mamoru Takahashi, Kiyotaka Nakagawa
{"title":"Effect of microemulsion system on water dispersibility and bioavailability of γ-oryzanol.","authors":"Junya Ito, Naoko Kumagai, Ayaka Suzuki, Naoki Shoji, Isabella Supardi Parida, Mamoru Takahashi, Kiyotaka Nakagawa","doi":"10.1093/bbb/zbaf002","DOIUrl":"10.1093/bbb/zbaf002","url":null,"abstract":"<p><p>This study developed water-dispersible γ-oryzanol (WD-OZ) using a microemulsion system and assessed their absorption in rats. While OZ itself is hardly soluble in water, WD-OZ exhibited high water dispersibility, and OZ, along with its metabolites, was detected in rat plasma. These findings provide a solid basis for future application of the microemulsion-based approach to enhance the bioavailability of OZ in food.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"633-637"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FRPR-1, a G protein-coupled receptor in the FMRFamide-related peptide receptor family, modulates larval development as a receptor candidate of the FMRFamide-like peptide FLP-1 in Caenorhabditis elegans. FRPR-1是fmrfamily相关肽受体家族中的G蛋白偶联受体（GPCR），在秀丽隐杆线虫中作为fmrfamily样肽FLP-1的候选受体调节幼虫的发育。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbaf004
Risako Une, Riko Uegaki, Sho Maega, Masahiro Ono, Tomohiro Bito, Takashi Iwasaki, Akira Shiraishi, Honoo Satake, Tsuyoshi Kawano
{"title":"FRPR-1, a G protein-coupled receptor in the FMRFamide-related peptide receptor family, modulates larval development as a receptor candidate of the FMRFamide-like peptide FLP-1 in Caenorhabditis elegans.","authors":"Risako Une, Riko Uegaki, Sho Maega, Masahiro Ono, Tomohiro Bito, Takashi Iwasaki, Akira Shiraishi, Honoo Satake, Tsuyoshi Kawano","doi":"10.1093/bbb/zbaf004","DOIUrl":"10.1093/bbb/zbaf004","url":null,"abstract":"<p><p>FMRFamide-like peptides (FLPs) and their receptors, FMRFamide-related peptide receptors (FRPRs) are widely conserved in free-living and parasitic nematodes. Herein, we identified FRPR-1 as an FLP-1 receptor candidate involved in larval development and diapause in the model nematode Caenorhabditis elegans. Our molecular genetic study, supported by in silico research, revealed the following: (1) frpr-1 loss-of-function completely suppresses the promotion of larval diapause caused by flp-1 overexpression; (2) AlphaFold2 analysis revealed the binding of FLP-1 to FRPR-1; (3) FRPR-1 as well as FLP-1 modulates the production and secretion of the predominant insulin-like peptide DAF-28, which is produced in ASI neurons; and (4) the suppression of larval diapause by frpr-1 loss-of-function is completely suppressed by a daf-28 defect. Thus, FRPR-1 regulates larval development and diapause by modulating DAF-28 production and secretion. This study may provide new insights into the development of novel nematicides targeting parasitic nematodes using FRPR-1 inhibitors.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"586-593"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plasmodium falciparum histidine-rich protein 2 exhibits cell penetration and cytotoxicity with autophagy dysfunction. 恶性疟原虫富组氨酸蛋白2表现出细胞渗透和细胞毒性，并伴有自噬功能障碍。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae209
Takashi Iwasaki, Mayu Shimoda, Haru Kanayama, Tsuyoshi Kawano
{"title":"Plasmodium falciparum histidine-rich protein 2 exhibits cell penetration and cytotoxicity with autophagy dysfunction.","authors":"Takashi Iwasaki, Mayu Shimoda, Haru Kanayama, Tsuyoshi Kawano","doi":"10.1093/bbb/zbae209","DOIUrl":"10.1093/bbb/zbae209","url":null,"abstract":"<p><p>Plasmodium falciparum is a major cause of severe malaria. This protozoan infects human red blood cells and secretes large quantities of histidine-rich protein 2 (PfHRP2) into the bloodstream, making it a well-known diagnostic marker. Here, however, we identified PfHRP2 as a pathogenic factor produced by P. falciparum. PfHRP2 showed cell penetration and cytotoxicity against various human cells. PfHRP2 also exhibited significant cytotoxicity at concentrations found in P. falciparum-infected patients' blood (90-100 n m). We also showed that PfHRP2 binds to Ca2+ ions, localizes to intracellular lysosomes, increases lysosomal Ca2+ levels, and inhibits the basal level of autophagy by preventing autolysosome formation. Furthermore, the Ca2+-dependent cytotoxicity of PfHRP2 was suppressed by the metal ion chelator ethylenediaminetetraacetic acid. In summary, our findings suggest PfHRP2 as a crucial pathogenic factor produced by P. falciparum and its mode of action. Overall, this study provides preliminary insights into P. falciparum malaria pathogenesis.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"548-561"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Manipulation and analysis of large DNA molecules by controlling their dynamics using micro and nanogaps. 操纵和分析大的DNA分子，通过控制他们的动态使用微和纳米间隙。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae179
Naoki Azuma
{"title":"Manipulation and analysis of large DNA molecules by controlling their dynamics using micro and nanogaps.","authors":"Naoki Azuma","doi":"10.1093/bbb/zbae179","DOIUrl":"10.1093/bbb/zbae179","url":null,"abstract":"<p><p>Manipulation and analysis methods for large DNAs are critical for epidemiological, clinical, diagnostic, and fundamental research on bacteria, membrane vesicles, plants, yeast, and human cells. However, the physical properties of large DNAs often challenge their manipulation and analysis with high accuracy and speed using conventional methods such as gel electrophoresis and column-based methods. This review presents the approaches that leverage micrometer- and nanometer-sized gaps within microchannels to control the dynamics and conformations of large DNAs, thereby overcoming these challenges. By designing gap structures and migration conditions based on the relationship between gap parameters and the physical characteristics of large DNAs-such as diameter and persistence length-these methods enable swifter and more precise manipulation and analysis of large DNAs, including size separation, concentration, purification, and single-molecule analysis.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"508-514"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic mechanisms underlying diverse panicle architecture in rice. 水稻穗型结构多样性的遗传机制。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-03-24 DOI: 10.1093/bbb/zbae189
Ayumi Agata
{"title":"Genetic mechanisms underlying diverse panicle architecture in rice.","authors":"Ayumi Agata","doi":"10.1093/bbb/zbae189","DOIUrl":"10.1093/bbb/zbae189","url":null,"abstract":"<p><p>Rice panicle architecture exhibits remarkable diversity and is crucial in determining grain production. Recent advances in the understanding of the genetic mechanisms underlying panicle morphogenesis offer promising avenues for improving rice productivity. Here, I reviewed recent studies on the developmental regulatory genes responsible for panicle architecture and explored how these findings can be applied to crop breeding. I also discuss the potential of using wild Oryza genetic resources, highlighting their value not only for scientific exploration but also for breeding innovation. Isolating novel genes related to panicle development and understanding their function are essential for designing diverse panicle architectures by quantitative trait locus pyramiding or genome editing technology. The use of these genetic resources offers a sustainable means to improve rice plant architecture and their resilience to climate change.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"502-507"},"PeriodicalIF":1.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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