Bioscience, Biotechnology, and Biochemistry最新文献_第2页

Different sterol compositions of honey bee Apis mellifera L. indicate role-dependent sterol metabolism. 蜜蜂不同的甾醇成分表明了角色依赖性的甾醇代谢。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag017

Makoto Ito, Hiroki Kohno, Hitomi Seike, Takao K Suzuki, Shinji Nagata

{"title":"Different sterol compositions of honey bee Apis mellifera L. indicate role-dependent sterol metabolism.","authors":"Makoto Ito, Hiroki Kohno, Hitomi Seike, Takao K Suzuki, Shinji Nagata","doi":"10.1093/bbb/zbag017","DOIUrl":"10.1093/bbb/zbag017","url":null,"abstract":"Honey bee Apis mellifera L. adults exhibit a sterol composition dominated by phytosterols such as 24-methylene-cholesterol and β-sitosterol, while most insects possess cholesterol as a primary sterol. To examine whether sterol composition varies by social roles, we analyzed nurse bees and foragers using gas chromatography-mass spectrometry. The analysis showed the four phytosterols, with 24-methylene-cholesterol being predominant. Honey, a dietary source for honey bees, contained more campesterol than in the whole body, suggesting possible metabolic use. Few significant differences in sterol compositions among tissues indicated that sterol metabolism is not tissue specific. However, social roles influenced different sterol compositions; nurse bees had higher β-sitosterol and isofucosterol, while foragers had more 24-methylene-cholesterol. Additionally, nurse bees retained more 24-methylene-cholesterol in their midgut. These findings suggest that sterol metabolism is influenced by social roles, probably due to differences in diet, absorption, or retention, rather than tissue dependent metabolism.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"587-596"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schisandrin A alleviates chondrocyte senescence and extracellular matrix degeneration by suppressing PI3K/Akt signaling pathway. 五味子素A通过抑制PI3K/Akt信号通路减轻软骨细胞衰老和细胞外基质变性。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag018

Kecun Chen, Danqiu Xu, Yunrong Ding, Yanjun Cao, Huajun Bo

{"title":"Schisandrin A alleviates chondrocyte senescence and extracellular matrix degeneration by suppressing PI3K/Akt signaling pathway.","authors":"Kecun Chen, Danqiu Xu, Yunrong Ding, Yanjun Cao, Huajun Bo","doi":"10.1093/bbb/zbag018","DOIUrl":"10.1093/bbb/zbag018","url":null,"abstract":"Osteoarthritis (OA) is a progressive joint disorder characterized by inflammation and metabolic imbalance. Schisandrin A (Sch-A), a bioactive compound from Schisandra sphenanthera, is known for its anti-inflammatory and protective properties. This study investigated the effects of Sch-A on chondrocyte senescence and metabolism using IL-1β-stimulated CHON-001 cells as an in vitro OA model. Sch-A showed no cytotoxicity up to 100 μm and alleviated IL-1β-induced chondrocyte injury. It restored anabolic metabolism, suppressed catabolic activity, and reduced inflammatory and fibrotic responses. Bioinformatics indicated links between Sch-A, cellular senescence, and the PI3K/Akt pathway. Functional assays confirmed that Sch-A suppressed senescence-associated secretory phenotype factors, senescence markers, SA-β-galactosidase activity, and PI3K/Akt activation, while PI3K inhibition enhanced its anti-senescent effects. These findings suggest that Sch-A mitigates chondrocyte senescence and metabolic dysregulation by modulating PI3K/Akt signaling, supporting its therapeutic potential for OA.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"624-632"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concise and enantioselective synthesis of (+)-SDEF 678 metabolite and (+)-speciosins A, C, and L. （+）-SDEF 678代谢物和(+)-物种蛋白A、C和L的简明和对映选择性合成。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag030

Kentaro Koyama, Hirosato Takikawa, Hironori Okamura

引用次数: 0

Induction of oxidative stress-associated cell death in HepG2 cells by combined methylmercury and palmitic acid exposure at low non-toxic concentrations. 低无毒浓度甲基汞和棕榈酸联合暴露诱导HepG2细胞氧化应激相关细胞死亡

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag027

Sora Toma, Genki Mizuno, Hiroya Yamada, Eiji Munetsuna, Takuya Wakasugi, Yuri Kamiya, Sei Nakanishi, Akira Kagami, Haruna Ishii, Yoshitaka Ando, Mirai Yamazaki, Hiroaki Ishikawa, Koji Suzuki, Koji Ohashi, Yoshiji Ohta, Mitsuhiro Okazaki

{"title":"Induction of oxidative stress-associated cell death in HepG2 cells by combined methylmercury and palmitic acid exposure at low non-toxic concentrations.","authors":"Sora Toma, Genki Mizuno, Hiroya Yamada, Eiji Munetsuna, Takuya Wakasugi, Yuri Kamiya, Sei Nakanishi, Akira Kagami, Haruna Ishii, Yoshitaka Ando, Mirai Yamazaki, Hiroaki Ishikawa, Koji Suzuki, Koji Ohashi, Yoshiji Ohta, Mitsuhiro Okazaki","doi":"10.1093/bbb/zbag027","DOIUrl":"10.1093/bbb/zbag027","url":null,"abstract":"Epidemiological and experimental studies have shown that low methylmercury (MeHg) exposure causes cytotoxic effects. As to such cytotoxic effects, we have supposed that not only MeHg itself but also MeHg interacting with living environmental factors may cause cytotoxic effects. MeHg exposure is known to induce oxidative stress and cell death via ferroptosis in hepatocytes. In this study, we examined whether MeHg exposure followed by palmitic acid (PA) exposure at low non-toxic concentrations cause oxidative stress and cell death in HepG2 cells. In HepG2 cells combinedly exposed to MeHg and PA at low non-toxic concentrations, cell viability and glutathione peroxidase 4 expression levels were significantly decreased, while reactive oxygen species level was significantly increased. Ferrostatin-1 pretreatment suppressed oxidative stress and cell death found in the HepG2 cells. These results indicate that combined exposure to MeHg and PA at low non-toxic concentrations induces oxidative stress associated cell death in HepG2 cells.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"711-719"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of differential phosphatidylcholine levels on growth and stress response in Acetobacter pasteurianus. 不同磷脂酰胆碱水平对巴氏醋酸杆菌生长和应激反应的影响。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag022

Yosuke Toyotake, Md Riad Hossain Khan, Shota Yamamoto, Yuichiro Hara, Yusuke Kawamata, Daisuke Matsui, Mamoru Wakayama

{"title":"Influence of differential phosphatidylcholine levels on growth and stress response in Acetobacter pasteurianus.","authors":"Yosuke Toyotake, Md Riad Hossain Khan, Shota Yamamoto, Yuichiro Hara, Yusuke Kawamata, Daisuke Matsui, Mamoru Wakayama","doi":"10.1093/bbb/zbag022","DOIUrl":"10.1093/bbb/zbag022","url":null,"abstract":"Acetic acid bacteria (AAB) produce phosphatidylcholine (PC) as a major membrane component. PC has long been considered important for acetic acid tolerance in AAB, yet direct experimental support remains limited, and its physiological roles under diverse environmental stresses are not well defined. In this study, we constructed PC-deficient mutants of Acetobacter pasteurianus by deleting the phosphatidylethanolamine N-methyltransferase gene. PC deficiency resulted in phosphatidylglycerol accumulation and a tendency toward acyl-chain shortening. Phenotypic analysis showed that PC deficiency impaired growth under ionic, heat, and acidic stresses, indicating that PC supports membrane integrity under these stress conditions. Heterologous expression of PC synthase from Pseudomonas aeruginosa enabled choline-dependent regulation of PC biosynthesis. This system revealed that even low PC levels are sufficient to restore normal growth and acetic acid fermentation. These findings suggest that PC has diverse physiological roles in AAB and its function does not necessarily depend on its abundance in the membrane.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"692-701"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146092089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional evaluation of perilla leaf tea as a pancreatic lipase inhibitor: roles of rosmarinic acid and emulsion structure. 紫苏叶茶作为胰脂肪酶抑制剂的功能评价：迷迭香酸和乳剂结构的作用。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag021

Mohammad Ariful Islam Bhuiya, Keisuke Yoshikiyo, Yukine Moda, Pinky Karim Syeda K Fatima, Kaeko Murota

{"title":"Functional evaluation of perilla leaf tea as a pancreatic lipase inhibitor: roles of rosmarinic acid and emulsion structure.","authors":"Mohammad Ariful Islam Bhuiya, Keisuke Yoshikiyo, Yukine Moda, Pinky Karim Syeda K Fatima, Kaeko Murota","doi":"10.1093/bbb/zbag021","DOIUrl":"10.1093/bbb/zbag021","url":null,"abstract":"Obesity is a growing global concern, and pancreatic lipase inhibitors have emerged as potential targets for antiobesity strategies. This study evaluates the potential of Perilla frutescens (L.) Britton var. frutescens leaf tea as a pancreatic lipase inhibitor for managing dietary fat absorption. Perilla leaf tea significantly inhibited the pancreatic lipase-mediated hydrolysis of emulsified triglycerides, depending on its polyphenol and rosmarinic acid (RA) contents. However, the inhibitory effect diminished at higher concentrations, which coincided with the increased emulsion particle size. Perilla leaf tea did not inhibit the hydrolysis of solubilized p-nitrophenyl palmitate, whereas authentic RA reduced lipase activity against both substrate types. These findings suggest that perilla leaf tea and RA have different lipase inhibition mechanisms. This study implies that RA contributes to the lipase-inhibiting properties of perilla leaf tea and emulsion structure plays a pivotal role in modulating the ability of the tea to inhibit lipase activity.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"671-678"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146092130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary purple nonsulfur bacteria increase body size of Caenorhabditis elegans. 饲料中紫色非硫细菌增加秀丽隐杆线虫的体型。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag012

W T R D Wikmal Subasinghe, Kohji Miyahara, Hitoshi Miyasaka, Hiroyuki Takeya

引用次数: 0

Insulin-like growth factor-1 regulates vascular endothelial growth factor synthesis and secretion in mammary epithelial cells. 胰岛素样生长因子-1调节乳腺上皮细胞血管内皮生长因子的合成和分泌。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag020

Asami Kawamura, Kei-Ichi Nakajima, Yuya Nagasawa, Yusaku Tsugami, So Shimoda, Megumi Kanao-Kanda, Takeshi Yamazaki, Yugo Watanabe, Takashi Yazawa, Jun-Ichi Kawabe

{"title":"Insulin-like growth factor-1 regulates vascular endothelial growth factor synthesis and secretion in mammary epithelial cells.","authors":"Asami Kawamura, Kei-Ichi Nakajima, Yuya Nagasawa, Yusaku Tsugami, So Shimoda, Megumi Kanao-Kanda, Takeshi Yamazaki, Yugo Watanabe, Takashi Yazawa, Jun-Ichi Kawabe","doi":"10.1093/bbb/zbag020","DOIUrl":"10.1093/bbb/zbag020","url":null,"abstract":"The mammary circulatory system supports mammary growth and lactation by supplying oxygen and nutrients. Insulin-like growth factor 1 (IGF1) is essential for mammary gland development, yet its involvement in angiogenic regulation remains unclear. This study investigated whether IGF1 stimulates vascular endothelial growth factor (VEGF) production in mammary epithelial cells and explored the underlying signaling pathways. IGF1 increased VEGF mRNA expression in a bovine mammary epithelial cell line and in HC11 cells, and enhanced VEGF promoter activity and secretion in HC11 cells in a dose-dependent manner. Conditioned medium from IGF1-stimulated HC11 cells promoted tube formation in HUVECs, which was attenuated by a VEGFR inhibitor. IGF1 rapidly induced ERK1/2 and Akt phosphorylation, and inhibitors of these pathways suppressed VEGF secretion. These findings suggest that IGF1 upregulates VEGF via the MEK-ERK1/2 and PI3K-Akt pathways, thereby promoting mammary angiogenesis and contributing to mammary gland development and lactation efficiency.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"616-623"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146092133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of autumn coloration of Acer palmatum leaves on their polyphenolic components and antioxidant activity. 掌叶秋季着色对其多酚成分及抗氧化活性的影响。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag016

Kenji Kuriya, Shiho Matsuura, Shunto Araga, Yuichi Masuda, Masahiro Nishio, Hayato Umekawa

引用次数: 0

Tryptanthrin, a constituent of Persicaria tinctoria, suppresses osteoclastogenesis and enhances the expression of tight junction proteins and cell adhesion molecules in gingival epithelial cells. 色氨酸是桃皮草的一种成分，它能抑制牙龈上皮细胞的破骨细胞生成，增强紧密连接蛋白和细胞粘附分子的表达。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-04-22 DOI: 10.1093/bbb/zbag025

Yukari Hatada, Yuji Inagaki, Daisuke Nakamura, Minato Akizuki, Yoshimune Iwata, Rie Kido, Mika Bando, Hiromichi Yumoto

{"title":"Tryptanthrin, a constituent of Persicaria tinctoria, suppresses osteoclastogenesis and enhances the expression of tight junction proteins and cell adhesion molecules in gingival epithelial cells.","authors":"Yukari Hatada, Yuji Inagaki, Daisuke Nakamura, Minato Akizuki, Yoshimune Iwata, Rie Kido, Mika Bando, Hiromichi Yumoto","doi":"10.1093/bbb/zbag025","DOIUrl":"10.1093/bbb/zbag025","url":null,"abstract":"Tryptanthrin, an ingredient of Persicaria tinctoria, has been reported to exhibit antioxidant and anti-inflammatory effects. To elucidate the potential role of tryptanthrin in periodontal disease, we investigated its effects on bone metabolism and innate immunity, including the epithelial barrier. RAW264.7 cells were cultured with soluble receptor activator of nuclear factor-κB ligand and tryptanthrin, and stained with tartrate-resistant acid phosphatase to evaluate osteoclastgenesis. The expression of osteoclast differentiation markers was examined using western blot analysis. OBA-9 cells were cultured with tryptanthrin, and the expression of tight junction (TJ) proteins and cell adhesion molecules (CAMs) was examined by western blot analysis. We found that tryptanthrin significantly inhibited osteoclastgenesis and suppressed the expression of osteoclast differentiation markers. Further, tryptanthrin enhanced the expression of TJ proteins and CAMs. These results indicated that tryptanthrin inhibits osteoclastgenesis by downregulating the osteoclast differentiation markers and enhances cell-cell adhesion by upregulating TJ proteins and CAMs.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"642-649"},"PeriodicalIF":1.3,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146156140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0