Bioscience, Biotechnology, and Biochemistry最新文献_第2页

Evaluation of synthetic peptides for direct protein delivery into Saccharomyces cerevisiae. 直接向酿酒酵母传递蛋白质的合成肽的评价。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf111

Shota Isogai, Ryoya Tanahashi, Hiroshi Takagi, Akira Nishimura

引用次数: 0

Transcriptional response to elemental sulfur in Geobacter sulfurreducens. 硫还原地杆菌对单质硫的转录反应。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf106

Masao Inoue, Daiki Fujita, Yukiko Izu, Riku Aono, Hisaaki Mihara

{"title":"Transcriptional response to elemental sulfur in Geobacter sulfurreducens.","authors":"Masao Inoue, Daiki Fujita, Yukiko Izu, Riku Aono, Hisaaki Mihara","doi":"10.1093/bbb/zbaf106","DOIUrl":"10.1093/bbb/zbaf106","url":null,"abstract":"The metal-reducing bacterium Geobacter sulfurreducens PCA is capable of anaerobic respiration using elemental sulfur as an electron acceptor. Despite 3 decades since its isolation, the molecular mechanisms underlying sulfur respiration remain unclear. In this study, we conducted a transcriptome analysis of G. sulfurreducens PCA cultured with and without sublimed sulfur. In the presence of sulfur, 153 genes were significantly up-regulated, while 599 genes were down-regulated. Notably, genes encoding redox proteins involved in energy conservation, particularly multiheme c-type cytochromes, exhibited altered expression patterns. In addition, elemental sulfur induced the transcription of genes associated with sulfur, selenium, and nitrogen metabolism, as well as protein redox homeostasis, DNA repair, and even cell motility. These transcriptional responses may reflect metabolic adaptation to sulfur respiration, redox state alterations, and sulfur-induced stress. Our findings uncover a complex regulatory landscape governing sulfur respiration and provide critical insights into this long-standing biochemical enigma in G. sulfurreducens PCA.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1499-1508"},"PeriodicalIF":1.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dehydrogenation of coelenterazine to dehydrocoelenterazine using molecular sieves. 用分子筛脱氢法制备coelenterazine。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf109

Mitsuhiro Nakamura, Kazuya Nakayama, Kazuo Matsuda, Satoshi Inouye

引用次数: 0

The aqueous extracts of Inonotus obliquus (Chaga) augment host defense against Listeria monocytogenes infection. 斜桦茸（Inonotus obliquus, Chaga）水提物增强宿主防御单核细胞增生李斯特菌感染。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf102

Akio Nakane, Tatsuji Takahashi, Kenichi Ito, Krisana Asano

{"title":"The aqueous extracts of Inonotus obliquus (Chaga) augment host defense against Listeria monocytogenes infection.","authors":"Akio Nakane, Tatsuji Takahashi, Kenichi Ito, Krisana Asano","doi":"10.1093/bbb/zbaf102","DOIUrl":"10.1093/bbb/zbaf102","url":null,"abstract":"Listeria monocytogenes, which grows in host cells intracellularly, is important as an opportunistic pathogen. Extracts of Chaga mushroom, Inonotus obliquus, have been shown to possess various biological activities. This study explores the impact of Chaga extracts on host defense against infection with L. monocytogenes, which induces T-helper (Th)1-type immune response. Chaga extracts enhanced cytokine production including tumor necrosis factor-α, interleukin (IL-6), and IL-12 but suppressed IL-10 production in murine macrophages and dendritic cells. Oral administration of Chaga extracts inhibited the proliferation of L. monocytogenes in the livers of infected mice. Interferon-γ (IFN-γ) and IL-12 production was enhanced in the spleen cell cultures of the extract-treated mice. Chaga extract treatment augmented the clearance of L. monocytogenes from the livers and spleens during re-infection. IFN-γ production was also enhanced in the spleen cell cultures of primarily infected mice. Together, the results indicate that Chaga extracts possess protective potentials for infectious diseases such as listeriosis.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1479-1487"},"PeriodicalIF":1.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human cathelicidin peptide LL-37 induces endothelial-to-mesenchymal transition. 人抗菌肽LL-37诱导内皮细胞向间质细胞转化。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf112

Kaori Suzuki, Mari Ohkuma, Isao Nagaoka

引用次数: 0

A key regulatory region required for the inhibition of proline utilization in the yeast transceptor Can1. 酵母受体Can1中抑制脯氨酸利用的关键调控区域。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf110

Ryoya Tanahashi, Hiroshi Takagi, Akira Nishimura

引用次数: 0

Palmitoylation of the fission yeast protein Isp3 is essential for the formation of the outermost layer of the spore wall. 裂变酵母蛋白Isp3的棕榈酰化对孢子壁最外层的形成至关重要。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf104

Takafumi Sakai, Nagisa Minomo, Tomoki Sakaguchi, Yuhei O Tahara, Makoto Miyata, Taro Nakamura

{"title":"Palmitoylation of the fission yeast protein Isp3 is essential for the formation of the outermost layer of the spore wall.","authors":"Takafumi Sakai, Nagisa Minomo, Tomoki Sakaguchi, Yuhei O Tahara, Makoto Miyata, Taro Nakamura","doi":"10.1093/bbb/zbaf104","DOIUrl":"10.1093/bbb/zbaf104","url":null,"abstract":"Fission yeast spores possess strong resistance to environmental stresses, largely due to the outermost proteinaceous \"Isp3 layer,\" which comprises Isp3 protein. Isp3 is palmitoylated, and its localization to the spore periphery is impaired in mutants lacking palmitoyltransferase; however, the precise role of Isp3 palmitoylation remains unclear. Here, we found that Isp3-GFP was expressed at wild-type levels in forming spores in mug142∆ cells lacking the palmitoyltransferase catalytic unit; thus, lack of palmitoylation did not reduce Isp3 protein stability. Next, we identified cysteine 7 as the key palmitoylation site essential for Isp3 localization to the spore periphery. Electron microscopy revealed that the Isp3 fibrillar layer was absent in both mug142Δ and isp3-C7S spores. Additionally, the isp3-C7S spores displayed increased sensitivity to alcohol stress, similar to isp3∆ spores. Collectively, these results demonstrate that palmitoylation of Isp3 is essential for the relocation of Isp3 to the spore surface and the assembly of the Isp3 layer.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1444-1455"},"PeriodicalIF":1.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Podophyllotoxin and α-peltatin inhibit nuclear factor κB activation and gene expression stimulated by a double-stranded RNA analogue in human umbilical vein endothelial cells. 足臼毒素和α-peltatin抑制双链RNA类似物刺激的人脐静脉内皮细胞核因子κB的激活和基因表达。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf108

Yuka Yokota, Piimwara Yarangsee, Kosuke Baba, Saki Sasaki, Hiroyuki Hirano, Hiroyuki Osada, Takao Kataoka

{"title":"Podophyllotoxin and α-peltatin inhibit nuclear factor κB activation and gene expression stimulated by a double-stranded RNA analogue in human umbilical vein endothelial cells.","authors":"Yuka Yokota, Piimwara Yarangsee, Kosuke Baba, Saki Sasaki, Hiroyuki Hirano, Hiroyuki Osada, Takao Kataoka","doi":"10.1093/bbb/zbaf108","DOIUrl":"10.1093/bbb/zbaf108","url":null,"abstract":"In vascular endothelial cells, proinflammatory cytokines and Toll-like receptor (TLR) ligands up-regulate the expression of adhesion molecules. In the present study, screening of the RIKEN Natural Products Depository chemical libraries identified podophyllotoxin and α-peltatin, which inhibited intercellular adhesion molecule-1 (ICAM-1) expression induced by polyinosinic-polycytidylic acid (Poly(I:C)) as a TLR3 ligand. In human umbilical vein endothelial cells (HUVEC), podophyllotoxin and its derivative α-peltatin inhibited Poly(I:C)-induced increases in the mRNA expression of ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. In addition, podophyllotoxin and α-peltatin inhibited the Poly(I:C)-induced nuclear translocation of the nuclear factor κB (NF-κB) subunit RelA. Microtubule-targeting agents (colchicine, vinblastine, and paclitaxel) exerted different effects on the Poly(I:C)-induced mRNA expression of ICAM-1, VCAM-1, and E-selectin. Vinblastine potently inhibited nuclear RelA translocation in Poly(I:C)-stimulated HUVEC, whereas colchicine and paclitaxel did not. Collectively, these results demonstrate that podophyllotoxin and α-peltatin inhibited NF-κB activation and mRNA expression by TLR3 stimulation in HUVEC.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1432-1443"},"PeriodicalIF":1.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and characterization of vitamin B12 compounds in traditional Japanese sweet "kuzu-mochi" made from fermented wheat starch. 由发酵小麦淀粉制成的日本传统甜“苦豆麻糬”中维生素B12化合物的存在和特征。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf113

Fumio Watanabe, Kyohei Koseki, Tomohiro Bito

引用次数: 0

Diversity and ecological roles of alginate lyases in marine and human microbial ecosystems. 海藻酸解酶在海洋和人类微生物生态系统中的多样性和生态作用。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-23 DOI: 10.1093/bbb/zbaf105

Kohei Ogura

引用次数: 0