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In vitro observation of histone-hexamer association with and dissociation from the amino-terminal region of budding yeast Mcm2, a subunit of the replicative helicase. 体外观察组蛋白六聚体与芽殖酵母 Mcm2（复制螺旋酶的一个亚基）氨基末端区域的结合和解离。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae109
Kohji Hizume
{"title":"In vitro observation of histone-hexamer association with and dissociation from the amino-terminal region of budding yeast Mcm2, a subunit of the replicative helicase.","authors":"Kohji Hizume","doi":"10.1093/bbb/zbae109","DOIUrl":"10.1093/bbb/zbae109","url":null,"abstract":"<p><p>During DNA replication, core histones that form nucleosomes on template strands are evicted and associate with newly synthesized strands to reform nucleosomes. Mcm2, a subunit of the Mcm2-7 complex, which is a core component of the replicative helicase, interacts with histones in the amino-terminal region (Mcm2N) and is involved in the parental histone recycling to lagging strands. Herein, the interaction of Mcm2N with histones was biochemically analyzed to reveal the molecular mechanisms underlying histone recycling by Mcm2N. With the addition of Mcm2N, a histone hexamer, comprising an H3-H4 tetramer and an H2A-H2B dimer, was excised from the histone octamer to form a complex with Mcm2N. The histone hexamer, but not H3-H4 tetramer was released from Mcm2N in the presence of Nap1, a histone chaperone. FACT, another histone chaperone, stabilized Mcm2N-histone hexamer complex to protect from Nap1-dependent dissociation. This study indicates cooperative histone transfer via Mcm2N and histone chaperones.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141892899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rice transcription factor DPF regulates stress-induced biosynthesis of diterpenoid phytoalexins. 水稻转录因子 DPF 调控胁迫诱导的二萜植物毒素的生物合成
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae118
Kazuki Ishikawa, Chihiro Yamamura, Koji Miyamoto, Yasukazu Kanda, Haruhiko Inoue, Kazunori Okada, Takashi Kamakura, Masaki Mori
{"title":"Rice transcription factor DPF regulates stress-induced biosynthesis of diterpenoid phytoalexins.","authors":"Kazuki Ishikawa, Chihiro Yamamura, Koji Miyamoto, Yasukazu Kanda, Haruhiko Inoue, Kazunori Okada, Takashi Kamakura, Masaki Mori","doi":"10.1093/bbb/zbae118","DOIUrl":"10.1093/bbb/zbae118","url":null,"abstract":"<p><p>Diterpenoid Phytoalexin Factor (DPF) is a key transcription factor involved in diterpenoid phytoalexin (DP) biosynthesis under non-stressed conditions in rice (Oryza sativa L.). Using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9, DPF knockout rice lines were generated. Treatments with abiotic stresses (copper chloride, ultraviolet light, and jasmonic acid) and biotic stress (blast fungus infection) to the knockout lines revealed that the DPF positively regulates stress-induced DP biosynthesis.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Subcellular compartmentalized localization of transmembrane proteins essential for production of fungal cyclic peptide cyclochlorotine. 生产真菌环肽环氯丁二烯所必需的跨膜蛋白的亚细胞区系定位。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae122
Takuya Katayama, Yulu Jiang, Taro Ozaki, Hideaki Oikawa, Atsushi Minami, Jun-Ichi Maruyama
{"title":"Subcellular compartmentalized localization of transmembrane proteins essential for production of fungal cyclic peptide cyclochlorotine.","authors":"Takuya Katayama, Yulu Jiang, Taro Ozaki, Hideaki Oikawa, Atsushi Minami, Jun-Ichi Maruyama","doi":"10.1093/bbb/zbae122","DOIUrl":"10.1093/bbb/zbae122","url":null,"abstract":"<p><p>Fungal biosynthetic gene clusters often include genes encoding transmembrane proteins, which have been mostly thought to be transporters exporting the products. However, there is little knowledge about subcellular compartmentalization of transmembrane proteins essential for biosynthesis. Fungal mycotoxin cyclochlorotine is synthesized by non-ribosomal peptide synthetase, which is followed by modifications with three transmembrane UstYa-family proteins. Heterologous expression in Aspergillus oryzae revealed that total biosynthesis of cyclochlorotine requires additional two transporter proteins. Here, we investigated subcellular localizations of the five transmembrane proteins under heterologous expression in A. oryzae. Enhanced green fluorescent protein (EGFP) fusions to the transmembrane proteins, which were confirmed to normally function in cyclochlorotine production, were expressed together with organellar markers. All the transmembrane proteins exhibited localizations commonly in line of the trans-Golgi, endosomes, and vacuoles. This study suggests that subcellular compartmentalization of UstYa family proteins and transporters allows corporative functions of delivering intermediates and subsequent modifications, completing cyclochlorotine biosynthesis.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and characterization of the suppressed lipid accumulation-related gene, SLA1, in the oleaginous yeast Lipomyces starkeyi. 油脂酵母星酵母中抑制脂质积累相关基因 SLA1 的鉴定和特征描述
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae107
Rikako Sato, Harutake Yamazaki, Kazuki Mori, Sachiyo Aburatani, Koji Ishiya, Yosuke Shida, Wataru Ogasawara, Kosuke Tashiro, Satoru Kuhara, Hiroaki Takaku
{"title":"Identification and characterization of the suppressed lipid accumulation-related gene, SLA1, in the oleaginous yeast Lipomyces starkeyi.","authors":"Rikako Sato, Harutake Yamazaki, Kazuki Mori, Sachiyo Aburatani, Koji Ishiya, Yosuke Shida, Wataru Ogasawara, Kosuke Tashiro, Satoru Kuhara, Hiroaki Takaku","doi":"10.1093/bbb/zbae107","DOIUrl":"10.1093/bbb/zbae107","url":null,"abstract":"<p><p>The oleaginous yeast Lipomyces starkeyi is an attractive industrial yeast that can accumulate high amounts of intracellular lipids. Identification of genes involved in lipid accumulation contributes not only to elucidating the lipid accumulation mechanism but also to breeding industrially useful high lipid-producing strains. In this study, the suppressed lipid accumulation-related gene (SLA1) was identified as the causative gene of the sr22 mutant with decreased lipid productivity. Suppressed lipid accumulation-related gene mutation reduced gene expression in lipid biosynthesis and increased gene expression in β-oxidation. Our results suggest that SLA1 mutation may leads to decreased lipid productivity. Suppressed lipid accumulation-related gene deletion also exhibited decreased gene expression in β-oxidation and increased lipid accumulation, suggesting that SLA1 deletion is a useful tool to improve lipid accumulation in L. starkeyi for industrialization.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Secondary metabolites from marine fungus Penicillium chrysogenum VH17 and their antimicrobial and cytotoxic potential. 海洋真菌青霉 VH17 的次级代谢产物及其抗菌和细胞毒性潜力。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae113
Nguyen Mai Anh, Le Thi Hong Minh, Nguyen Thuy Linh, Phi Thi Dao, Do Thi Quynh, Doan Thi Mai Huong, Pham Van Cuong, Vu Thi Thu Huyen, Ton That Huu Dat
{"title":"Secondary metabolites from marine fungus Penicillium chrysogenum VH17 and their antimicrobial and cytotoxic potential.","authors":"Nguyen Mai Anh, Le Thi Hong Minh, Nguyen Thuy Linh, Phi Thi Dao, Do Thi Quynh, Doan Thi Mai Huong, Pham Van Cuong, Vu Thi Thu Huyen, Ton That Huu Dat","doi":"10.1093/bbb/zbae113","DOIUrl":"10.1093/bbb/zbae113","url":null,"abstract":"<p><p>One new compound, methyl 3-((1-((2-carbamoylphenyl)amino)-1-oxopropan-2-yl)amino)-3-oxopropanoate (1), along with 9 known secondary metabolites (2-10) were isolated and elucidated chemical structures from the methanol extract of the marine-derived fungus Penicillium chrysogenum VH17. Subsequent bioassays showed the antimicrobial and cytotoxic potential of the isolated compounds. All compounds 1-10 displayed antimicrobial effects against at least one tested reference microorganism with MIC values ranging from 32 to 256 µg mL-1. Furthermore, compound 4 exhibited significant cytotoxicity against all tested cell lines, HepG2, A549, and MCF7 with IC50 values of 29.43 ± 1.37, 33.02 ± 1.53, and 36.72 ± 1.88 µM, respectively, whereas compound 3 exhibited weak cytotoxicity against MCF7 and HepG2 cell lines with IC50 values of 87.17 ± 6.31 and 97.32 ± 5.66 µM, respectively.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glycosylphosphatidylinositol-anchored lipid transfer proteins influence root cap cuticle formation at primary root tips, promoting NaCl tolerance in Arabidopsis seedlings. 糖基磷脂酰肌醇锚定脂质转移蛋白影响主根顶端根帽角质层的形成，促进拟南芥幼苗对 NaCl 的耐受性。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae117
Yuta Uemura, Satomi Sakaoka, Atsushi Morikami, Hironaka Tsukagoshi
{"title":"Glycosylphosphatidylinositol-anchored lipid transfer proteins influence root cap cuticle formation at primary root tips, promoting NaCl tolerance in Arabidopsis seedlings.","authors":"Yuta Uemura, Satomi Sakaoka, Atsushi Morikami, Hironaka Tsukagoshi","doi":"10.1093/bbb/zbae117","DOIUrl":"10.1093/bbb/zbae117","url":null,"abstract":"<p><p>Root cap cuticles (RCCs), comprising mainly very-long-chain fatty acids (VLCFAs), promote salt tolerance by preventing ion influx. Glycosylphosphatidylinositol-anchored lipid transfer protein (LTPG)1 and LTPG2 participate in VLCFA deposition in the extracellular region, aiding RCC formation in the lateral roots. In this study, we investigated whether LTPG1 and LTPG2 have similar functions in the primary roots of young Arabidopsis thaliana. Phenotypic analyses, fluorescence microscopy, and quantitative real-time reverse transcription polymerase chain reaction confirmed that NaCl exposure induced LTPG1 and LTPG2 expression and promoted RCC formation in young primary roots. The loss of RCC in the ltpg1 and ltpg2 mutants resulted in increased NaCl sensitivity of root elongation. NaCl also upregulated the expression of several NaCl-responsive genes in ltpg1 and ltpg2. We conclude that RCC formation via LTPG function is pivotal in enhancing salt tolerance in young primary roots.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142118980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of bacteria from Grifola frondosa cultivation on wood logs to find mycelial growth-promoting bacteria. 从木质原木上栽培的蕨类植物中分离细菌，寻找促进菌丝生长的细菌。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae116
Fu-Chia Chen, Ichiro Kamei
{"title":"Isolation of bacteria from Grifola frondosa cultivation on wood logs to find mycelial growth-promoting bacteria.","authors":"Fu-Chia Chen, Ichiro Kamei","doi":"10.1093/bbb/zbae116","DOIUrl":"10.1093/bbb/zbae116","url":null,"abstract":"<p><p>This study aimed to isolate bacteria that coexist with the edible mushroom Grifola frondosa when it is cultured on wood, and to determine their interactions; in turn, the aim was to find bacteria that stimulate mycelial growth so as to decrease the time required for spawn preparation on potato dextrose agar (PDA). Some Pseudomonas, Dyella, Bacillus, and Priestia spp. isolated from the cultivation surroundings of G. frondosa had a positive effect on the mycelial growth of the fungus in PDA. However, some isolated bacteria had a severe negative effect on the mycelial growth, especially Burkholderia spp. Thus, both mycelial-promoting bacteria and potentially pathogenic bacteria coexist with G. frondosa in cultivation. Enzyme activity assays indicated that some wood-degrading bacteria inhabit the cultivation surroundings of G. frondosa, and these bacteria probably help the fungus to degrade wood (especially cellulose).</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbial production of aromatic compounds and synthesis of high-performance bioplastics. 微生物生产芳香族化合物和合成高性能生物塑料。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae111
Shunsuke Masuo
{"title":"Microbial production of aromatic compounds and synthesis of high-performance bioplastics.","authors":"Shunsuke Masuo","doi":"10.1093/bbb/zbae111","DOIUrl":"10.1093/bbb/zbae111","url":null,"abstract":"<p><p>Microbial fermentation has provided fermented foods and important chemicals such as antibiotics, amino acids, and vitamins. Metabolic engineering of synthetic microbes has expanded the range of compounds produced by fermentation. Petroleum-derived aromatic compounds are widely used in industry as raw materials for pharmaceuticals, dyes, and polymers and are in great demand. This review highlights the current efforts in the microbial production of various aromatic chemicals such as aromatic amines, cinnamic acid derivatives, and flavoring aromatics, including their biosynthesis pathways. In addition, the unique biosynthetic mechanism of pyrazine, a heterocyclic compound, from amino acids is described to expand the use of biomass-derived aromatic compounds. I also discuss our efforts to develop high-performance bioplastics superior to petroleum plastics from the aromatic compounds produced by microbial fermentation.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibitory effects of extracts from Eucalyptus gunnii on α-synuclein amyloid fibrils. 枪叶桉提取物对α-突触核蛋白淀粉样纤维的抑制作用
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae114
Masatomo So, Misaki Ono, Shigeki Oogai, Minako Kondo, Kaede Yamazaki, Charlotte Nachtegael, Hiroshi Hamajima, Risa Mutoh, Masaki Kato, Hisaya Kawate, Tomoyuki Oki, Yasushi Kawata, Shiho Kumamoto, Noritaka Tokui, Toshiki Takei, Kuniyoshi Shimizu, Akio Inoue, Naoki Yamamoto, Motoko Unoki, Kenichi Tanabe, Kinichi Nakashima, Hiroyuki Sasaki, Hironobu Hojo, Yasuo Nagata, Isao Suetake
{"title":"Inhibitory effects of extracts from Eucalyptus gunnii on α-synuclein amyloid fibrils.","authors":"Masatomo So, Misaki Ono, Shigeki Oogai, Minako Kondo, Kaede Yamazaki, Charlotte Nachtegael, Hiroshi Hamajima, Risa Mutoh, Masaki Kato, Hisaya Kawate, Tomoyuki Oki, Yasushi Kawata, Shiho Kumamoto, Noritaka Tokui, Toshiki Takei, Kuniyoshi Shimizu, Akio Inoue, Naoki Yamamoto, Motoko Unoki, Kenichi Tanabe, Kinichi Nakashima, Hiroyuki Sasaki, Hironobu Hojo, Yasuo Nagata, Isao Suetake","doi":"10.1093/bbb/zbae114","DOIUrl":"10.1093/bbb/zbae114","url":null,"abstract":"<p><p>Amyloid fibril formation is associated with various amyloidoses, including neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Despite the numerous studies on the inhibition of amyloid formation, the prevention and treatment of a majority of amyloid-related disorders are still challenging. In this study, we investigated the effects of various plant extracts on amyloid formation of α-synuclein. We found that the extracts from Eucalyptus gunnii are able to inhibit amyloid formation, and to disaggregate preformed fibrils, in vitro. The extract itself did not lead to cell damage. In the extract, miquelianin, which is a glycosylated form of quercetin and has been detected in the plasma and the brain, was identified and assessed to have a moderate inhibitory activity, compared to the effects of ellagic acid and quercetin, which are strong inhibitors for amyloid formation. The properties of miquelianin provide insights into the mechanisms controlling the assembly of α-synuclein in the brain.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular modification of chlorogenic acid via radiolysis with inhibitory effects on NO production. 通过辐射分解对绿原酸进行分子修饰，从而抑制 NO 的产生。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI: 10.1093/bbb/zbae123
Gyeong Han Jeong, Hanui Lee, Kyung-Bon Lee, Byung Yeoup Chung, Hyoung-Woo Bai
{"title":"Molecular modification of chlorogenic acid via radiolysis with inhibitory effects on NO production.","authors":"Gyeong Han Jeong, Hanui Lee, Kyung-Bon Lee, Byung Yeoup Chung, Hyoung-Woo Bai","doi":"10.1093/bbb/zbae123","DOIUrl":"10.1093/bbb/zbae123","url":null,"abstract":"<p><p>The molecular modification of chlorogenic acid (1) through γ-irradiation resulted in the formation of five new products: chlorogenosins A (2), B (3), C (4), D (5), and E (6) along with known compounds rosmarinosin B (7), protocatechuic acid (8), and protocatechuic aldehyde (9). The structures of the new compounds were elucidated using spectroscopic methods, including one-dimensional and two-dimensional nuclear magnetic resonance, high-resolution electrospray ionization mass spectroscopy, and circular dichroism spectroscopy. The potential anti-inflammatory activities of all the isolated compounds were determined by evaluating their inhibitory effects on the nitric oxide (NO) production in lipopolysaccharide-induced RAW 264.7 macrophages. Notably, compounds 2 and 3, which contained two hydroxymethyl functionalities instead of the trans-olefinic moiety present in the original chlorogenic acid, exhibited stronger inhibitory effects on NO production than that of the original compound. These findings suggest that the predominant chemical changes induced in chlorogenic acid by γ-irradiation may enhance its anti-inflammatory properties.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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