Bioscience, Biotechnology, and Biochemistry最新文献_第3页

PNGase activity and free N-glycans in phloem fluid prepared from Nerium oleander (oleander tree). 夹竹桃韧皮液中PNGase活性及游离n -聚糖的测定。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-21 DOI: 10.1093/bbb/zbaf038

Fuki Otaguro, Yoshinobu Kimura, Megumi Maeda

引用次数: 0

Combinatory breeding of sake yeast strains with mutations that enhance Ginjo aroma production. 具有增强银杏香气突变的清酒酵母菌的组合选育。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-21 DOI: 10.1093/bbb/zbaf029

Yifeng Gong, Norapat Klinkaewboonwong, Risa Hayashi, Yan Zhou, Ikuhisa Nishida, Rei Saito, Tetsuya Goshima, Tomoyuki Nishi, Daisuke Watanabe, Dai Hirata, Takeshi Akao, Yoshikazu Ohya

{"title":"Combinatory breeding of sake yeast strains with mutations that enhance Ginjo aroma production.","authors":"Yifeng Gong, Norapat Klinkaewboonwong, Risa Hayashi, Yan Zhou, Ikuhisa Nishida, Rei Saito, Tetsuya Goshima, Tomoyuki Nishi, Daisuke Watanabe, Dai Hirata, Takeshi Akao, Yoshikazu Ohya","doi":"10.1093/bbb/zbaf029","DOIUrl":"10.1093/bbb/zbaf029","url":null,"abstract":"Isoamyl acetate and ethyl caproate are the primary aroma compounds responsible for the fruity fragrance characteristic of Ginjo sake. Simultaneous high-level production of both compounds is crucial to achieving a balanced aroma and complex flavor. Isoamyl acetate is predominantly produced by hda1∆/hda1∆ and LEU4(G516S)/LEU4(G516S), while ethyl caproate is produced in high quantities by FAS2(G1250S)/FAS2(G1250S). In this study, to maximize the production of both aroma compounds, genome editing was employed to generate sake yeast strains combining these mutations. After small-scale fermentation tests were conducted to evaluate the production of aroma compounds, we found that the isoamyl acetate-enhancing effect of hda1∆/hda1∆ was almost completely masked by FAS2(G1250S)/FAS2(G1250S). In contrast, the effects of LEU4(G516S)/LEU4(G516S) were not entirely masked by FAS2(G1250S)/FAS2(G1250S), resulting in 2.4- and 5.4-fold greater production of isoamyl acetate and ethyl caproate, respectively. This study highlights the utility of genome editing in the combinatorial breeding of sake yeast.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"910-917"},"PeriodicalIF":1.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T7 expression system in Bacillus subtilis utilizing the rhiL promoter responsive to pectin and glucose. 利用rhiL启动子在枯草芽孢杆菌中对果胶和葡萄糖响应的T7表达系统。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-21 DOI: 10.1093/bbb/zbaf035

Yuna Kuwahara, Kazutake Hirooka

引用次数: 0

Synthesis of homocarnosine for applications in bioactive peptide and functional food studies. 正肌肽的合成及其在生物活性肽和功能性食品研究中的应用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-20 DOI: 10.1093/bbb/zbaf075

Tomoka Nagao, Kanako Sato, Takeshi Arima, Takumi Komaru, Sudathip Sae-Tan, Masubon Thongngam, Hisato Kuniyoshi, Makoto Hirayama, Yoshiaki Nakamura, Akiko Koi, Jun-Ichi Kakimura, Hitoshi Fujitaka, Ruge Cao, Noriyuki Yanaka, Thanutchaporn Kumrungsee

引用次数: 0

Evidence that abscisic acid mediates gene activation under dehydrating conditions in pearl millet. 脱落酸介导珍珠粟脱水条件下基因活化的证据。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-20 DOI: 10.1093/bbb/zbaf070

Maimuna Qazi, Kota Kambara, Shashi Kumar Gupta, Tetsuo Takano, Daisuke Tsugama

引用次数: 0

Deficiency of BTB and CNC Homology 1 promotes colon tumorigenesis by enhancing intestinal epithelial cell proliferation in mice. BTB和CNC同源物1缺乏通过增强小鼠肠上皮细胞增殖促进结肠肿瘤的发生。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-19 DOI: 10.1093/bbb/zbaf073

Karen Kanamaru, Jun Tanikawa, Tomohisa Takagi, Akihiko Muto, Kazuhiko Igarashi, Yuji Naito, Yasuki Higashimura

{"title":"Deficiency of BTB and CNC Homology 1 promotes colon tumorigenesis by enhancing intestinal epithelial cell proliferation in mice.","authors":"Karen Kanamaru, Jun Tanikawa, Tomohisa Takagi, Akihiko Muto, Kazuhiko Igarashi, Yuji Naito, Yasuki Higashimura","doi":"10.1093/bbb/zbaf073","DOIUrl":"https://doi.org/10.1093/bbb/zbaf073","url":null,"abstract":"Intestinal epithelial cells serve as the frontline of host defense and function as a physical barrier. BTB and CNC homology 1 (Bach1) is a transcriptional repressor involved in gastrointestinal physiology, but its role in epithelial proliferation and colorectal tumorigenesis remains unclear. This study demonstrates that Bach1 deficiency promotes colonic epithelial proliferation and enhances colorectal tumorigenesis. In Bach1-deficient (Bach1-/-) mice, structural analysis revealed elongation of villi in the small intestine and pronounced changes in the colon, with increased Ki67+ and BrdU+ cells indicating hyperproliferation. In HT-29 colon cancer cells, BACH1 knockdown accelerated cell cycle progression by reducing G0/G1-phase cells and increasing S-phase and G2/M-phase cells, accompanied by upregulation of cell cycle-related genes. Additionally, in an azoxymethane-induced colorectal carcinogenesis model, Bach1-/- mice exhibited a significant increase in aberrant crypt foci formation. These findings suggest that Bach1 deficiency contributes to colorectal tumorigenesis by promoting epithelial hyperproliferation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced Heat Resistance in Rhodococcus Strains via Alkane Phase Translocation in Aqueous-Alkane Two-phase Cultures. 在水-烷烃两相培养中通过烷烃相易位诱导红球菌的耐热性。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-16 DOI: 10.1093/bbb/zbaf074

Noriyuki Iwabuchi, Yuki Tsuchiya, Tomoko Aizawa-Urai, Jun Ogihara

引用次数: 0

Enhancement of carbohydrate metabolism by probiotic and prebiotic intake promotes short-chain fatty acid production in the gut microbiome: A randomized, double-blind, placebo-controlled crossover trial. 益生菌和益生元摄入促进碳水化合物代谢促进肠道微生物组短链脂肪酸的产生：一项随机、双盲、安慰剂对照交叉试验

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-14 DOI: 10.1093/bbb/zbaf071

Yuhei Baba, Daisuke Tsuge, Ryo Aoki

{"title":"Enhancement of carbohydrate metabolism by probiotic and prebiotic intake promotes short-chain fatty acid production in the gut microbiome: A randomized, double-blind, placebo-controlled crossover trial.","authors":"Yuhei Baba, Daisuke Tsuge, Ryo Aoki","doi":"10.1093/bbb/zbaf071","DOIUrl":"https://doi.org/10.1093/bbb/zbaf071","url":null,"abstract":"Short-chain fatty acids (SCFAs) are thought to be a key factor in the health benefits of prebiotics and probiotics. This study investigated the effect of Bifidobacterium animalis subsp. lactis GCL2505 and inulin (BL+IN) on fecal SCFAs and gut microbial composition and function. A placebo-controlled, randomized, double-blind, crossover study was conducted with 120 participants. Two weeks of BL+IN intake significantly increased acetate, propionate, and butyrate concentrations and Bifidobacterium abundance compared to placebo. Functional analysis of the gut metagenome showed enrichment of genes associated with carbohydrate and amino acid metabolism, suggesting an enhanced capacity for SCFA production. A responder was defined as a participant with increased fecal SCFAs after BL+IN consumption. Responder metagenomes exhibited greater enrichment of genes involved in SCFA production and carbohydrate metabolism. In conclusion, short-term BL+IN ingestion may benefit healthy adults by increasing fecal SCFAs through influencing the composition and functional activation of SCFA-associated pathways in the gut microbiome.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1,6-Digalloyl glucose in Emblica officinalis increases the proliferation of human keratinocytes and fibroblasts with upregulated expression of periostin. officinalis中的1,6-二烯丙基葡萄糖增加人角质形成细胞和成纤维细胞的增殖，并上调骨膜蛋白的表达。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-09 DOI: 10.1093/bbb/zbaf068

Yoshimi Yamakami, Kyoko Morino, Robert A Kanaly, Raku Irie, Masato Oikawa, Yuki Takauji, Kensuke Miki, Mohammad Nazir Hossain, Dai Ayusawa, Michihiko Fujii

{"title":"1,6-Digalloyl glucose in Emblica officinalis increases the proliferation of human keratinocytes and fibroblasts with upregulated expression of periostin.","authors":"Yoshimi Yamakami, Kyoko Morino, Robert A Kanaly, Raku Irie, Masato Oikawa, Yuki Takauji, Kensuke Miki, Mohammad Nazir Hossain, Dai Ayusawa, Michihiko Fujii","doi":"10.1093/bbb/zbaf068","DOIUrl":"https://doi.org/10.1093/bbb/zbaf068","url":null,"abstract":"Keratinocytes and fibroblasts are the major cell types in the epidermis and the dermis, respectively. Since both cells play important roles in wound healing, compounds that regulate their proliferation are expected to be beneficial to epidermal health. We have previously shown that the extract of Emblica officinalis, which is a well-known herb in Ayurvedic traditional medicine, increases the proliferation of keratinocytes. Here we purified a compound that increased cell proliferation from E. officinalis extract. Through liquid chromatography electrospray ionization tandem mass spectrometry and NMR techniques, the compound was determined to be 1,6-digalloyl glucose. Consistent with this, tannic acid, a type of polygalloyl glucose, also increased cell proliferation. We further found that E. officinalis extract increased the proliferation of fibroblasts as well and showed that it increased cell proliferation at least partly through upregulating the expression of periostin. These findings revealed the mechanism by which E. officinalis extract regulates cell proliferation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143979261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved emulsifying property and reduced immunogenicity of β-lactoglobulin by conjugation with carboxymethyl cellulose of different molecular weight. 通过与不同分子量的羧甲基纤维素偶联，改善了β-乳球蛋白的乳化性能，降低了免疫原性。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-05-09 DOI: 10.1093/bbb/zbaf069

Moeko Ono, Tadashi Yoshida, Makoto Hattori

引用次数: 0