Bioscience, Biotechnology, and Biochemistry最新文献_第3页

Utilization of duckweed-derived biomass for polyhydroxybutyrate production in Escherichia coli via dual enzymatic saccharification using amylase and cellulase complex. 通过使用淀粉酶和纤维素酶复合物进行双酶糖化，在大肠杆菌中利用浮萍生物质生产多羟基丁酸。

IF 1.6 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-12 DOI: 10.1093/bbb/zbae127

Toshihiko Ooi,Rikako Sato,Ken'ichiro Matsumoto

引用次数: 0

Decomposition of brown algae in the ocean by microbiota: biological insights for recycling blue carbon. 微生物群分解海洋中的褐藻：回收蓝碳的生物学启示。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-05 DOI: 10.1093/bbb/zbae126

Natsuko Katsuhiro, Kanomi Sato, Ryuichi Takase, Shigeyuki Kawai, Kohei Ogura, Wataru Hashimoto

{"title":"Decomposition of brown algae in the ocean by microbiota: biological insights for recycling blue carbon.","authors":"Natsuko Katsuhiro, Kanomi Sato, Ryuichi Takase, Shigeyuki Kawai, Kohei Ogura, Wataru Hashimoto","doi":"10.1093/bbb/zbae126","DOIUrl":"https://doi.org/10.1093/bbb/zbae126","url":null,"abstract":"Brown algae are one of the most abundant biomasses on Earth. To recycle them as blue carbon sources, an effective decomposition system is necessary. This study focused on microorganisms present in seawater that decompose brown algae which contain laminarin and alginate. Where Undaria and Sargassum spp. were present, genera Psychromonas, Psychrobacter, and Pseudoalteromonas were predominant in seawater, while genera Arcobacter and Fusobacterium increased in abundance during the process of decomposition. The inoculation of Undaria samples into laminarin-minimal media led to a predominance of Pseudoalteromonas species. A Pseudoalteromonas isolate, identified as Pseudoalteromonas distincta, possesses genes encoding a putative laminarinase, polysaccharide lyase family 6 (PL6) alginate lyases, and a PL7 alginate lyase. The culture media of P. distincta contained no monosaccharides, suggesting the rapid conversion of polysaccharides to metabolites. These findings indicated that Pseudoalteromonas species play a major role in the decomposition of brown algae and affect the microbiota associated with them.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of sesquiterpene aldehydes as volatile antifungal compounds in Phaeolepiota aurea culture filtrate. 鉴定 Phaeolepiota aurea 培养滤液中的倍半萜醛类挥发性抗真菌化合物。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-05 DOI: 10.1093/bbb/zbae125

Kota Seki, Tomoya Tanaka, Emiko Shimoda, Shinji Tanio, Ryo C Yanagita, Tsugumi Miyazaki, Kento Tokumoto, Toshiaki Tazawa, Kumiko Osaki-Oka, Atsushi Ishihara

{"title":"Identification of sesquiterpene aldehydes as volatile antifungal compounds in Phaeolepiota aurea culture filtrate.","authors":"Kota Seki, Tomoya Tanaka, Emiko Shimoda, Shinji Tanio, Ryo C Yanagita, Tsugumi Miyazaki, Kento Tokumoto, Toshiaki Tazawa, Kumiko Osaki-Oka, Atsushi Ishihara","doi":"10.1093/bbb/zbae125","DOIUrl":"https://doi.org/10.1093/bbb/zbae125","url":null,"abstract":"The culture filtrate extract of golden bootleg (Phaeolepiota aurea) exhibited strong antifungal activity in a bioassay for volatile compounds. Purification of active compounds through silica gel column chromatography and HPLC isolated two compounds, 1 and 2, with molecular weights of 234 and 259, respectively, as confirmed by mass spectrometry. NMR analysis identified these compounds as new sesquiterpenoids with the α-bourbonene skeleton. Both compounds had an aldehyde group, while 2 possessed a cyanohydrin group. These compounds were named phaeolep aldehydes A (1) and B (2). Phaeolep aldehyde A inhibited hyphae elongation of Penicillium citrinum, Aspergillus niger, Cladosporium sphaerospermum, and Alternaria brassicicola at 10 ppm, but did not exhibit any antibacterial activity. In contrast, phaeolep aldehyde B exhibited potent inhibitory effects on the spore germination of Pe. citrinum, achieving 58.2% inhibition at 0.25 ppm, outperforming phaeolep aldehyde A, which exhibited 25.7% inhibition at 10 ppm.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of physiological roles of UDP-glycosyltransferase UGT76F2 in auxin homeostasis through the TAA-YUCCA auxin biosynthesis pathway. 研究 UDP-糖基转移酶 UGT76F2 通过 TAA-YUCCA auxin 生物合成途径在 auxin 平衡中的生理作用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-04 DOI: 10.1093/bbb/zbae124

Mio Harada, Tomoaki Kubotsu, Takemoto Agui, Xinhua Dai, Yunde Zhao, Hiroyuki Kasahara, Ken-Ichiro Hayashi

{"title":"Investigation of physiological roles of UDP-glycosyltransferase UGT76F2 in auxin homeostasis through the TAA-YUCCA auxin biosynthesis pathway.","authors":"Mio Harada, Tomoaki Kubotsu, Takemoto Agui, Xinhua Dai, Yunde Zhao, Hiroyuki Kasahara, Ken-Ichiro Hayashi","doi":"10.1093/bbb/zbae124","DOIUrl":"https://doi.org/10.1093/bbb/zbae124","url":null,"abstract":"Cellular auxin (indole-3-acetic acid, IAA) levels are coordinately regulated by IAA biosynthesis and inactivation. IAA is synthesized through sequential reactions by two enzymes, TAA1 and YUCCA, in a linear indole-3-pyruvic acid (IPA) pathway. TAA1 converts tryptophan to IPA, and YUCCA catalyzes the oxidative decarboxylation of IPA into IAA. Arabidopsis UDP-glycosyltransferase UGT76F2 (At3g55710) was previously reported to catalyze the glycosylation of IPA and consequently modulate IAA levels. We carefully analyzed the physiological roles of UGT76F2 and its close homolog UGT76F1 (At3g55700) in IAA homeostasis. We generated two independent ugt76f1 ugt76f2 double null Arabidopsis mutants (ugt76f1f2) with a 2.7 kb deletion, along with two independent ugt76f2 single null mutants by CRISPR/Cas9 gene editing technology. Surprisingly, these null mutants exhibited indistinguishable phenotypes from the wild-type seedlings under our laboratory conditions. Our results indicate that UGT76F1 and UGT76F2 do not play important roles in regulating IAA biosynthesis via the IPA glycosylation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcellular compartmentalized localization of transmembrane proteins essential for production of fungal cyclic peptide cyclochlorotine. 生产真菌环肽环氯丁二烯所必需的跨膜蛋白的亚细胞区系定位。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-04 DOI: 10.1093/bbb/zbae122

Takuya Katayama, Yulu Jiang, Taro Ozaki, Hideaki Oikawa, Atsushi Minami, Jun-Ichi Maruyama

{"title":"Subcellular compartmentalized localization of transmembrane proteins essential for production of fungal cyclic peptide cyclochlorotine.","authors":"Takuya Katayama, Yulu Jiang, Taro Ozaki, Hideaki Oikawa, Atsushi Minami, Jun-Ichi Maruyama","doi":"10.1093/bbb/zbae122","DOIUrl":"https://doi.org/10.1093/bbb/zbae122","url":null,"abstract":"Fungal biosynthetic gene clusters often include genes encoding transmembrane proteins, which have been mostly thought to be transporters exporting the products. However, there is little knowledge about subcellular compartmentalization of transmembrane proteins essential for biosynthesis. Fungal mycotoxin cyclochlorotine is synthesized by non-ribosomal peptide synthetase, which is followed by modifications with three transmembrane UstYa-family proteins. Heterologous expression in Aspergillus oryzae revealed that total biosynthesis of cyclochlorotine requires additional two transporter proteins. Here, we investigated subcellular localizations of the five transmembrane proteins under heterologous expression in A. oryzae. Enhanced green fluorescent protein (EGFP) fusions to the transmembrane proteins, which were confirmed to normally function in cyclochlorotine production, were expressed together with organellar markers. All the transmembrane proteins exhibited localizations commonly in line of the trans-Golgi, endosomes, and vacuoles. This study suggests that subcellular compartmentalization of UstYa family proteins and transporters allows corporative functions of delivering intermediates and subsequent modifications, completing cyclochlorotine biosynthesis.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular modification of chlorogenic acid via radiolysis with inhibitory effects on NO production. 通过辐射分解对绿原酸进行分子修饰，从而抑制 NO 的产生。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-04 DOI: 10.1093/bbb/zbae123

Gyeong Han Jeong, Hanui Lee, Kyung-Bon Lee, Byung Yeoup Chung, Hyoung-Woo Bai

{"title":"Molecular modification of chlorogenic acid via radiolysis with inhibitory effects on NO production.","authors":"Gyeong Han Jeong, Hanui Lee, Kyung-Bon Lee, Byung Yeoup Chung, Hyoung-Woo Bai","doi":"10.1093/bbb/zbae123","DOIUrl":"https://doi.org/10.1093/bbb/zbae123","url":null,"abstract":"The molecular modification of chlorogenic acid (1) through γ-irradiation resulted in the formation of five new products: chlorogenosins A (2), B (3), C (4), D (5), and E (6) along with known compounds rosmarinosin B (7), protocatechuic acid (8), and protocatechuic aldehyde (9). The structures of the new compounds were elucidated using spectroscopic methods, including one-dimensional and two-dimensional nuclear magnetic resonance, high-resolution electrospray ionization mass spectroscopy, and circular dichroism spectroscopy. The potential anti-inflammatory activities of all the isolated compounds were determined by evaluating their inhibitory effects on the nitric oxide production in lipopolysaccharide-induced RAW 264.7 macrophages. Notably, compounds 2 and 3, which contained two hydroxymethyl functionalities instead of the trans-olefinic moiety present in the original chlorogenic acid, exhibited stronger inhibitory effects on NO production than that of the original compound. These findings suggest that the predominant chemical changes induced in chlorogenic acid by γ-irradiation may enhance its anti-inflammatory properties.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice Transcription Factor DPF Regulates Stress-Induced Biosynthesis of Diterpenoid Phytoalexins. 水稻转录因子 DPF 调控胁迫诱导的二萜植物毒素的生物合成

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-03 DOI: 10.1093/bbb/zbae118

Kazuki Ishikawa, Chihiro Yamamura, Koji Miyamoto, Yasukazu Kanda, Haruhiko Inoue, Kazunori Okada, Takashi Kamakura, Masaki Mori

引用次数: 0

Glycosylphosphatidylinositol-anchored lipid transfer proteins influence root cap cuticle formation at primary root tips, promoting NaCl tolerance in Arabidopsis seedlings. 糖基磷脂酰肌醇锚定脂质转移蛋白影响主根顶端根帽角质层的形成，促进拟南芥幼苗对 NaCl 的耐受性。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-02 DOI: 10.1093/bbb/zbae117

Yuta Uemura, Satomi Sakaoka, Atsushi Morikami, Hironaka Tsukagoshi

引用次数: 0

Bacillus subtilis grown in a "breathing" vessel without sparger aeration. 枯草芽孢杆菌在 "会呼吸 "的容器中生长，不使用喷射器通气。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-09-02 DOI: 10.1093/bbb/zbae120

Ken-Ichi Yoshida, Kyosuke Yokoyama, Ayşegül Öktem, Shu Ishikawa, Jan Maarten van Dijl, Masato Yotsuya, Ryosuke Sato

引用次数: 0

Effects of acetic acid fermentation product and its components on defense signaling in rice. 醋酸发酵产物及其成分对水稻防御信号的影响

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-08-31 DOI: 10.1093/bbb/zbae121

Koharu Abe, Manae Mori, Akira Nakayama

引用次数: 0