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Identification of peptaibols as specific inhibitors of the futalosine pathway. 富塔苷途径特异性抑制肽的鉴定。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf050
Marie Kikuchi, Haruka Kiyanagi, Takeshi Tsunoda, Yuki Inahashi, Kenichi Nonaka, Tohru Dairi, Yasushi Ogasawara
{"title":"Identification of peptaibols as specific inhibitors of the futalosine pathway.","authors":"Marie Kikuchi, Haruka Kiyanagi, Takeshi Tsunoda, Yuki Inahashi, Kenichi Nonaka, Tohru Dairi, Yasushi Ogasawara","doi":"10.1093/bbb/zbaf050","DOIUrl":"10.1093/bbb/zbaf050","url":null,"abstract":"<p><p>Menaquinone is an indispensable cofactor in the electron-transfer pathway for most Gram-positive bacteria. While most bacteria biosynthesize menaquinone by the well-known canonical pathway, some bacteria including Helicobacter pylori, which is a pathogen causing stomach cancer, use the futalosine pathway. Because beneficial intestinal bacteria such as lactobacilli use the canonical pathway, the futalosine pathway will be a great target for developing antibiotics specific to H. pylori. In this study, we searched for such metabolites from actinomycetes and fungi and identified 4 new peptaibols from culture broth of Verruciconidia sp. FKI-8918 as specific inhibitors of the futalosine pathway.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"985-989"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression in HEK293 cells, purification, and characterization of recombinant human α-glucosidases. 重组人α-葡萄糖苷酶在HEK293细胞中的表达、纯化和表征。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf055
So Nishimoto, Teisuke Takita, Kazumichi M Nishida, Masaaki Ito, Toshiyuki Kimura, Yasuhisa Kimura, Noriyuki Kioka, Kiyoshi Yasukawa
{"title":"Expression in HEK293 cells, purification, and characterization of recombinant human α-glucosidases.","authors":"So Nishimoto, Teisuke Takita, Kazumichi M Nishida, Masaaki Ito, Toshiyuki Kimura, Yasuhisa Kimura, Noriyuki Kioka, Kiyoshi Yasukawa","doi":"10.1093/bbb/zbaf055","DOIUrl":"10.1093/bbb/zbaf055","url":null,"abstract":"<p><p>In mammals, intestinal α-glucosidase exists within maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) complexes. Here, we purified recombinant human MGAM and SI from human embryonic kidney 293 cells using Flag tag-based affinity chromatography. Steady-state kinetic analysis at pH 6.0 and 37 °C showed that the maltase activities of MGAM and SI were higher than their sucrase and isomaltase activities.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"990-994"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Honey flavors formed via yeast fermentation in honey from Japanese honeybees. 日本蜜蜂的蜂蜜通过酵母发酵形成蜂蜜风味。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf057
Hiroyuki Iguchi, Akihisa Watanabe
{"title":"Honey flavors formed via yeast fermentation in honey from Japanese honeybees.","authors":"Hiroyuki Iguchi, Akihisa Watanabe","doi":"10.1093/bbb/zbaf057","DOIUrl":"10.1093/bbb/zbaf057","url":null,"abstract":"<p><p>Honey is formed from floral nectar through bee-derived substances, dehydration, and chemical reactions during storage in beehives. While bacteria and fungi inhabit honey and beehives, their roles in honey maturation remain unclear. In this study, we characterized the fermentation process of honey from Apis cerana japonica (Japanese honeybee) with respect to its microbial and flavor compound profiles. Metagenomic analysis revealed that the fungi in Japanese honeybee honey are dominated by Zygosaccharomyces siamensis, with minor members of Talaromyces, Oidiodendron, Starmerella, and Priceomyces. Fermentation of diluted raw honey increased the population of Z. siamensis. Inoculating honey with Z. siamensis produced aromatic compounds, including isoamyl alcohol, hotrienol, 2-phenylethanol, and 2-phenylethyl acetate, as well as the organic acid succinate. These results indicate that fermentation by Z. siamensis can generate favorable flavor compounds, offering the potential for enhancing honey's sensory qualities and applications in the food industry.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1062-1069"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant growth regulators from the liquid culture of Lepista sordida. 黄连液体培养植物生长调节剂的研究。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf048
Arbin Sunuwar, Jae-Hoon Choi, Masaaki Sumida, Kokoro Kume, Jing Wu, Akinobu Ito, Kimiko Yamashita, Hirofumi Hirai, Hirokazu Kawagishi
{"title":"Plant growth regulators from the liquid culture of Lepista sordida.","authors":"Arbin Sunuwar, Jae-Hoon Choi, Masaaki Sumida, Kokoro Kume, Jing Wu, Akinobu Ito, Kimiko Yamashita, Hirofumi Hirai, Hirokazu Kawagishi","doi":"10.1093/bbb/zbaf048","DOIUrl":"10.1093/bbb/zbaf048","url":null,"abstract":"<p><p>\"Fairy rings\" are zones that stimulate or inhibit grass growth, caused by interactions between a fungus and a grass. Lepista sordida is one of the fairy ring-forming fungi. (1S,2S)-1-(4-chloro-3-methoxyphenyl)propane-1,2-diol (1) along with 10 compounds (2-11) was isolated from the culture broth and mycelia of L. sordida. The structures were identified by spectroscopic data analyses, and this was the first reported isolation of 1 from a natural source. The plant growth regulatory activity of 3-6 was investigated using bentgrass seedlings. Compound 3 promoted root growth at 0.1 μmol/paper, while inhibited it at 1 μmol/paper. Compound 4 inhibited root growth at 0.1 and 1 μmol/paper. Compound 6 significantly promoted shoot growth at 1 μmol/paper.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"934-940"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RASL10A, a member of the RAS subfamily of small GTPases, suppresses the EMT and invasion of human lung adenocarcinoma A549 cells. RASL10A是小GTPases的RAS亚家族成员，可抑制人肺腺癌A549细胞的EMT和侵袭。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf052
Makoto Nishizuka, Kanae Nakajima, Chie Odagiri, Nanaka Wada, Satoshi Sakai
{"title":"RASL10A, a member of the RAS subfamily of small GTPases, suppresses the EMT and invasion of human lung adenocarcinoma A549 cells.","authors":"Makoto Nishizuka, Kanae Nakajima, Chie Odagiri, Nanaka Wada, Satoshi Sakai","doi":"10.1093/bbb/zbaf052","DOIUrl":"10.1093/bbb/zbaf052","url":null,"abstract":"<p><p>Lung adenocarcinoma (LUAD) is the most frequently diagnosed form of non-small cell lung cancer and is known to develop distant metastases, such as brain and bone metastases, which can considerably affect the prognosis of patients. Therefore, there is a need to elucidate the metastatic mechanisms of LUAD and establish treatments to control metastasis. In this study, Kaplan-Meier plotter analysis demonstrated that patients with LUAD and low RASL10A expression have a poor prognosis. Furthermore, loss or gain of function experiments revealed that RASL10A suppresses transforming growth factor-beta (TGF-β)-induced epithelial-mesenchymal transition (EMT) and the subsequent invasion of LUAD A549 cells. In addition, we found that RASL10A suppresses Smad3 phosphorylation and Snail expression during the early stages of EMT. These results indicate that RASL10A is a novel factor that inhibits TGF-β signaling-mediated EMT and invasion in A549 cells. RASL10A has the potential to become a new therapeutic target for patients with LUAD.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"945-953"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Breeding of the sake yeast K1801 with normal checkpoint integrity by isolating spontaneous cdc55 revertants. 通过分离cdc55自然复归物选育具有正常检查点完整性的清酒酵母K1801。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf089
Tetsuya Goshima, Ikuhisa Nishida, Hitoshi Shimoi, Takeshi Akao, Dai Hirata
{"title":"Breeding of the sake yeast K1801 with normal checkpoint integrity by isolating spontaneous cdc55 revertants.","authors":"Tetsuya Goshima, Ikuhisa Nishida, Hitoshi Shimoi, Takeshi Akao, Dai Hirata","doi":"10.1093/bbb/zbaf089","DOIUrl":"https://doi.org/10.1093/bbb/zbaf089","url":null,"abstract":"<p><p>Sake yeast strain K1801 with high productivity of ethyl caproate is widely used for high-quality sake brewing, however K1801 has a defect in spindle assembly checkpoint (SAC), whose responsible mutation is R48P of the PP2A regulatory subunit Cdc55. Here, we successfully isolated spontaneous revertants of the cdc55 mutation with the excellent brewing property similar to K1801 and normal checkpoint integrity.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glycolaldehyde affects the growth of and metabolic flux distribution in Synechocystis sp. PCC 6803. 乙二醛影响 Synechocystis sp. PCC 6803 的生长和代谢通量分布。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf046
Tatsumi Imada, Yoshihiro Toya, Hiroshi Shimizu
{"title":"Glycolaldehyde affects the growth of and metabolic flux distribution in Synechocystis sp. PCC 6803.","authors":"Tatsumi Imada, Yoshihiro Toya, Hiroshi Shimizu","doi":"10.1093/bbb/zbaf046","DOIUrl":"10.1093/bbb/zbaf046","url":null,"abstract":"<p><p>Glycolaldehyde (GA) is widely used as a photosynthetic inhibitor to suppress metabolic reactions. We aimed to evaluate the effects of GA on the growth of and metabolic flux distribution in Synechocystis sp. PCC 6803. GA significantly inhibited the growth [half-maximal inhibitory concentration (IC50) = 244 μm] and metabolic flux through the Calvin-Benson-Bassham cycle without decrease in flux of the pentose phosphate pathway.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"995-999"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nutritional and functional profiles of aquaponic leaf lettuce across seasons. 水培叶莴苣不同季节的营养和功能特征。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI: 10.1093/bbb/zbaf051
Yasuaki Enoki, Satomi Tsutsuura, Keigo Mikame, Norikuni Ohtake, Tomoko Yamaguchi
{"title":"Nutritional and functional profiles of aquaponic leaf lettuce across seasons.","authors":"Yasuaki Enoki, Satomi Tsutsuura, Keigo Mikame, Norikuni Ohtake, Tomoko Yamaguchi","doi":"10.1093/bbb/zbaf051","DOIUrl":"10.1093/bbb/zbaf051","url":null,"abstract":"<p><p>Aquaponics is a sustainable food production system that combines aquaculture and hydroponics. However, limited studies have focused on the nutritional and functional properties of aquaponic produce. Thus, this study aimed to explore the nutritional and functional components of 4 leaf lettuce lines with different colors cultivated in a commercial aquaponic farm in 4 seasons from summer 2022 to spring 2023. The leaf lettuce lines grown in aquaponics had high levels of micronutrients such as vitamin C, vitamin K, and folic acid, with the levels of vitamin K and folic acid meeting Japan's health claim criteria. The lettuce lines, especially red leaf lettuce, were also rich in bioactive compounds such as nitrate ions, which are linked to blood pressure regulation, and antioxidants such as anthocyanins and chlorogenic acid. These findings suggest that leaf lettuce grown in aquaponics has potential use as a high-value functional food.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1024-1037"},"PeriodicalIF":1.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a Novel Fatty Acid-Derived Biopolymer with Tunable Polarity. 一种新型极性可调脂肪酸衍生生物聚合物的研制。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-20 DOI: 10.1093/bbb/zbaf095
Takeshi Imai, Hiroto Achira, Akihiko Tanitame, Hisaaki Mihara
{"title":"Development of a Novel Fatty Acid-Derived Biopolymer with Tunable Polarity.","authors":"Takeshi Imai, Hiroto Achira, Akihiko Tanitame, Hisaaki Mihara","doi":"10.1093/bbb/zbaf095","DOIUrl":"https://doi.org/10.1093/bbb/zbaf095","url":null,"abstract":"<p><p>A novel biopolymer, fatty acid-derived polyelectrolyte (FADP), was synthesized via oxidative polymerization and base-induced ionization of unsaturated fatty acids. We evaluated its ionization degree, molecular weight progression, and mechanical performance. FADP composites with optimal carboxylate content exhibited high flexural strength, suggesting the potential of producing sustainable, polarity-tunable polymers through a simple process.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery and characterization of high-activity formate oxidase with low Km value under non-acidic conditions. 非酸性条件下低Km值高活性甲酸氧化酶的发现与表征。
IF 1.4 4区 生物学
Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-19 DOI: 10.1093/bbb/zbaf092
Zhenyu Zhai, Yi-Heng P Job Zhang
{"title":"Discovery and characterization of high-activity formate oxidase with low Km value under non-acidic conditions.","authors":"Zhenyu Zhai, Yi-Heng P Job Zhang","doi":"10.1093/bbb/zbaf092","DOIUrl":"https://doi.org/10.1093/bbb/zbaf092","url":null,"abstract":"<p><p>Formate oxidase (FOX) has applications in enzymatic assays, pollutant biodegradation, and bioorganic chemistry. However, its sources are few and none of them have significant activity above pH 7. We performed bioinformatic mining of FOX and identified 579 potential sequences. Two FOXs from Aspergillus nomiae (AnFOX) and Trichophyton rubrum (TrFOX) were expressed in E. coli. AnFOX had an alkalinity-tolerant pH activity range. It not only had a Km value for formate one order of magnitude lower, but also had far higher catalytic activity under non-acidic conditions. Spectroscopic and mass spectrometry analyses found that AnFOX contains an 8-formyl FAD cofactor. AnFOX enabled the H2O2-dependent sulfoxidation of thioanisole over pH 6-8. Structural analysis and kinetic studies revealed that the acidic residue E142, positioned on the surface adjacent to the isoalloxazine ring of flavin, could contribute to the unique pH preference. This novel FOX could greatly expand the applicability under non-acidic conditions.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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