Bioscience, Biotechnology, and Biochemistry最新文献_第4页

Gene identification of L-alanine-3-formylquinuclidine aminotransferase from Brevibacterium aurantiacum NBRC 12171. 金短杆菌nbrc12171 l -丙氨酸-3-甲酰基喹啉转氨酶基因的鉴定

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-24 DOI: 10.1093/bbb/zbag143

Yuki Okumura, Hiroyuki Jomen, Michiki Takeuchi, Shoko Kozono, Ryotaro Hara, Tomotsune Onaga, Tetsuji Noda, Seiichiro Matsumoto, Makoto Ueda, Jun Ogawa

引用次数: 0

Biochemical analysis of catalytic and disease-associated residues in mouse Golgi-resident 3'-phosphoadenosine-5'-phosphate (PAP) phosphatase BPNT2/gPAPP. 小鼠高尔基- 3′-磷酸腺苷-5′-磷酸（PAP）磷酸酶BPNT2/gPAPP催化残基和疾病相关残基的生化分析

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-24 DOI: 10.1093/bbb/zbag144

Ryota Urushihara, Yasuhisa Maruno, Saki Maruoka, Moe Tsuruta, Ryotaro Matsuzaki, Etsuko Nishimoto, Takamasa Teramoto, Yoshimitsu Kakuta

{"title":"Biochemical analysis of catalytic and disease-associated residues in mouse Golgi-resident 3'-phosphoadenosine-5'-phosphate (PAP) phosphatase BPNT2/gPAPP.","authors":"Ryota Urushihara, Yasuhisa Maruno, Saki Maruoka, Moe Tsuruta, Ryotaro Matsuzaki, Etsuko Nishimoto, Takamasa Teramoto, Yoshimitsu Kakuta","doi":"10.1093/bbb/zbag144","DOIUrl":"https://doi.org/10.1093/bbb/zbag144","url":null,"abstract":"Golgi-resident 3'-phosphoadenosine-5'-phosphate (PAP) phosphatase BPNT2/gPAPP plays an essential role in maintaining sulfation homeostasis, but its biochemical characterization has been limited by the difficulty of in vitro analyses. Here, we established a stable expression and activity assay system using a Trigger factor-fused BPNT2 construct. Using this system, we performed AlphaFold 3-guided mutational analyses. The predicted model and mutational analyses showed that several acidic residues are required for efficient PAP phosphatase activity, likely through their contribution to Mg²⁺ coordination. In addition, T177 was suggested to help maintain an active-site geometry that could accommodate a catalytic water molecule. Conservation analysis and lithium inhibition supported the idea that BPNT2 shares a conserved Mg²⁺-dependent catalytic framework with related PAP phosphatases. Disease-associated variants D175N and T181P showed substantially reduced activity, possibly due to impaired Mg2+ coordination and local structural distortion. These findings provide a biochemical framework for understanding BPNT2 function in Golgi sulfation regulation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of a malic acid-overproducing sake yeast that does not possess the known mutations causing high-malic acid-production. 一株苹果酸高产清酒酵母的分离，该酵母不具有引起苹果酸高产的已知突变。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-21 DOI: 10.1093/bbb/zbag142

Tomoyuki Nishi, Ikuhisa Nishida, Takeshi Akao, Dai Hirata

引用次数: 0

Selective curing of the endogenous plasmid pSF3 encoding organophosphorus hydrolase in Sphingobium fuliginis ATCC 27551. 黄颡鱼ATCC 27551有机磷水解酶内源质粒pSF3的选择性固化。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-16 DOI: 10.1093/bbb/zbag141

Satoshi Ishikawa, Natsumi Ishikawa, Satoshi Onodera, Yuji Kikuchi, Kazuyoshi Kawahara

引用次数: 0

Elucidating causes of fouling of canned black coffee manufacturing equipment. 黑咖啡罐头生产设备结垢原因分析。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-15 DOI: 10.1093/bbb/zbag138

Md Sajjad Hossain, Tokio Takahashi, Sho Ogasawara, Mario Shibata, Tomoaki Hagiwara

引用次数: 0

The Digestive Fate and Intestinal Transport Pathway of Parvalbumin Underlying Its Strong Allergenicity. 小白蛋白强致敏性背后的消化命运和肠道转运途径。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-14 DOI: 10.1093/bbb/zbag140

Nobuhiro Sudo, Ga-Hyun Joe, Hiroki Saeki, Kazuhiko Watanabe, Yutaka Shimizu

{"title":"The Digestive Fate and Intestinal Transport Pathway of Parvalbumin Underlying Its Strong Allergenicity.","authors":"Nobuhiro Sudo, Ga-Hyun Joe, Hiroki Saeki, Kazuhiko Watanabe, Yutaka Shimizu","doi":"10.1093/bbb/zbag140","DOIUrl":"https://doi.org/10.1093/bbb/zbag140","url":null,"abstract":"Parvalbumin, the major fish allergen, exhibits allergenicity, yet its digestive and absorptive behavior in vivo remains poorly understood. This study evaluated digestion-dependent changes in IgE reactivity and the in vivo behavior of parvalbumin from salmon, flounder, and herring. In vitro, parvalbumin was rapidly cleaved by pepsin-trypsin, but the resulting low-molecular-weight fragments retained substantial IgE reactivity. In mice, orally administered parvalbumin was also digested in the gastrointestinal tract, although part of the protein persisted in the small intestine for extended periods. Notably, parvalbumin traversed the cytoplasm of intestinal epithelial cells and rapidly appeared in the venous bloodstream, indicating transcellular absorption. These findings were consistent across the three species, suggesting that the persistence of parvalbumin's IgE reactivity despite extensive digestive fragmentation, together with its sustained intestinal uptake, is associated with its strong allergenic potential. This study provides key insights into the mechanisms linking digestive and absorptive processes to parvalbumin allergenicity.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heating duration alters the low-molecular-weight compound profile of fish muscle, with pyruvic acid as a potential marker of thermal history. 加热时间改变了鱼肌肉的低分子量化合物分布，丙酮酸是热历史的潜在标志。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-12 DOI: 10.1093/bbb/zbag139

Ayumi Furuta, Ryu Sugimasab, Miyu Saba, Shota Tanimoto, Ryota Mabuchi

引用次数: 0

Exploratory analysis of the associations of short-chain fatty acids and their related factors with growth performance in growing pigs using a Bayesian network. 利用贝叶斯网络探索性分析短链脂肪酸及其相关因素与生长猪生长性能的关系。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-10 DOI: 10.1093/bbb/zbag137

Toru Takahashi, Takamitsu Tsukahara, Hiroto Miura, Takahiro Kawase, Shin-Ichi Nakamura, Shozo Tomonaga, Kikuto Fukuta, Ryo Inoue

{"title":"Exploratory analysis of the associations of short-chain fatty acids and their related factors with growth performance in growing pigs using a Bayesian network.","authors":"Toru Takahashi, Takamitsu Tsukahara, Hiroto Miura, Takahiro Kawase, Shin-Ichi Nakamura, Shozo Tomonaga, Kikuto Fukuta, Ryo Inoue","doi":"10.1093/bbb/zbag137","DOIUrl":"https://doi.org/10.1093/bbb/zbag137","url":null,"abstract":"We suggested the causal relationships between daily weight gains (DG) and feed conversion ratios (FCR) of growing pigs, and short-chain fatty acids (SCFA) concentrations in digesta and blood, and SCFA-related factors such as intestinal morphology, SCFA transporters and tight junction (TJ) proteins. Twelve pigs were divided into 5% of rapeseed meal-, beet pulp- or starch-supplemented groups, kept for 21 days, and dissected. Bayesian network showed that factors affecting DG were expressions of sodium-coupled monocarboxylate transporter 1, claudin-1, and Zonula occludens-1 in cecal mucosa, monocarboxylate transporter 1 and occludin in ileal mucosa, and SCFA concentrations in abdominal aortas. Factors affecting FCR were type of diet, villous height in ileum, SCFA concentrations in ileal veins and cecal digesta. Greater DG may be associated with stimulation of SCFA transportation and regulation of TJ functions, whereas reduced FCR appears to be linked to villous elongation and stimulation of SCFA production in the intestine.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capric Acid Attenuates Palmitate-Induced Lipotoxicity and Metabolic Dysfunction in Skeletal Muscle Cells. 癸酸减轻棕榈酸诱导的脂肪毒性和骨骼肌细胞代谢功能障碍。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-09 DOI: 10.1093/bbb/zbag136

Kenjiro Furusho, Wakako Tawara, Shunsuke Amagaya, Ayumu Kojima, Risa Mukai, Atsushi Kuno, Azusa Tomioka, Shinji Okada, Tsukasa Mori, Nobuhiro Zaima, Naoko Goto-Inoue

{"title":"Capric Acid Attenuates Palmitate-Induced Lipotoxicity and Metabolic Dysfunction in Skeletal Muscle Cells.","authors":"Kenjiro Furusho, Wakako Tawara, Shunsuke Amagaya, Ayumu Kojima, Risa Mukai, Atsushi Kuno, Azusa Tomioka, Shinji Okada, Tsukasa Mori, Nobuhiro Zaima, Naoko Goto-Inoue","doi":"10.1093/bbb/zbag136","DOIUrl":"https://doi.org/10.1093/bbb/zbag136","url":null,"abstract":"Skeletal muscle insulin resistance and atrophy are associated with lipid overload and lipotoxicity. The effects of medium-chain fatty acids on skeletal muscle lipotoxicity are unclear. Here, we investigated the effects of capric acid (C10) on palmitate-induced dysfunction in C2C12 myotubes by evaluating their morphology, lipid accumulation, contractile activity, and insulin signaling, and performing lipidomic and proteomic analyses. Palmitate increased lipid droplet accumulation, impaired myotube integrity and contractility, and reduced insulin-stimulated Akt phosphorylation. Concurrent C10 treatment suppressed lipid accumulation and restored myotube morphology, contractile activity, and insulin responsiveness. Lipidomic analyses revealed that C10 reduced palmitate-containing ceramide and triacylglycerol species while increasing C10-containing triacylglycerols. Proteomics revealed the enrichment of cytoskeletal, motor protein, glycolytic, and tricarboxylic acid cycle pathways and downregulation of diabetic cardiomyopathy-related pathways. Furthermore, C10 pre-treatment partially protected against palmitate-induced dysfunction. These findings suggest that C10 attenuates palmitate-induced muscle dysfunction by promoting lipid, metabolic, and structural remodeling.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preventive intervention with prebiotic resistant maltodextrin mitigates dextran sulfate sodium-induced colitis in female mice. 抗益生元麦芽糖糊精预防干预可减轻硫酸葡聚糖钠诱导的雌性小鼠结肠炎。

IF 1.5 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-08 DOI: 10.1093/bbb/zbag134

Kyoka Yamazaki, Akane Kanasaki, Machiko Kitagawa, Yusuke Sone, Yuka Kishimoto

{"title":"Preventive intervention with prebiotic resistant maltodextrin mitigates dextran sulfate sodium-induced colitis in female mice.","authors":"Kyoka Yamazaki, Akane Kanasaki, Machiko Kitagawa, Yusuke Sone, Yuka Kishimoto","doi":"10.1093/bbb/zbag134","DOIUrl":"https://doi.org/10.1093/bbb/zbag134","url":null,"abstract":"Ulcerative colitis (UC), an inflammatory bowel disease with a rising global prevalence, may be alleviated by short-chain fatty acids (SCFAs) derived from dietary fiber fermentation. Additionally, preventive fiber intake, rather than therapeutic, reportedly attenuates clinical and inflammatory markers of experimental colitis. To investigate the effects of preventive intake of resistant maltodextrin (RMD), a soluble dietary fiber, on colitis, female C57BL/6 mice received 6% RMD for 28 days before dextran sulfate sodium (DSS) administration. RMD mitigated colitis, as evaluated by reducing disease activity index (DAI), body weight loss, colon shortening. It increased SCFAs, particularly butyrate, before DSS treatment, with butyrate showing a negative correlation trend with DAI and a positive correlation with colon length. RMD tended to increase Fusicatenibacter saccharivorans, known to decrease in active UC, after DSS treatment, which was negatively correlated with DAI. Taken together, preventive RMD intake may attenuate experimental colitis, possibly through increased butyrate and F. saccharivorans.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0