Bioscience, Biotechnology, and Biochemistry最新文献_第4页

Effects of elevated yet non-inhibitory levels of selenite on growth and gene expression of the methanogenic archaeon Methanococcus maripaludis JJ. 亚硒酸盐对产甲烷古菌maripaludis产甲烷球菌生长和基因表达的影响

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-08 DOI: 10.1093/bbb/zbaf131

Riku Aono, Mikihisa Onoda, Masao Inoue, Anna Ochi, Hisaaki Mihara

{"title":"Effects of elevated yet non-inhibitory levels of selenite on growth and gene expression of the methanogenic archaeon Methanococcus maripaludis JJ.","authors":"Riku Aono, Mikihisa Onoda, Masao Inoue, Anna Ochi, Hisaaki Mihara","doi":"10.1093/bbb/zbaf131","DOIUrl":"https://doi.org/10.1093/bbb/zbaf131","url":null,"abstract":"Selenium is an essential trace element in many organisms but becomes toxic at elevated concentrations. At moderately increased, non-lethal levels, selenite triggers both selenium utilization and stress responses in microorganisms. However, the thresholds of such responses in archaea remain poorly understood. Here, we investigated the effects of elevated yet non-inhibitory concentrations of selenite on growth and gene expression in the methanogenic archaeon Methanococcus maripaludis JJ. We observed no significant impact on growth rate or cell yield, even at 100 µM selenite. RNA sequencing and reverse transcription-quantitative polymerase chain reaction analyses revealed differential expression of genes involved in methanogenesis and transport systems, which may contribute to maintaining growth under elevated selenite. Our findings suggest that the transcriptional response to selenite in M. maripaludis is initiated at low concentrations (e.g., <1 µM) and remains saturated across a hundredfold range (up to 100 µM), without inducing stress responses or impairing growth.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sebacic acid promotes osteoblast differentiation. 癸二酸促进成骨细胞分化。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-05 DOI: 10.1093/bbb/zbaf128

Thira Rojasawasthien, Takumi Ito, Hideto Okamoto, Nobuaki Okumura, Sachiko Yamashita Takeuchi, Tomohiko Shirakawa, Takuma Matsubara, Shoichiro Kokabu

引用次数: 0

Inulin Exacerbates Disease Severity in a Mouse Model of Ulcerative Colitis by Causing Osmotic Diarrhea. 菊粉通过引起渗透性腹泻加重溃疡性结肠炎小鼠模型的疾病严重程度。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-04 DOI: 10.1093/bbb/zbaf129

Takashi Hosono, Keigo Saito, Yu Arima, Yori Ozaki-Masuzawa, Taiichiro Seki

{"title":"Inulin Exacerbates Disease Severity in a Mouse Model of Ulcerative Colitis by Causing Osmotic Diarrhea.","authors":"Takashi Hosono, Keigo Saito, Yu Arima, Yori Ozaki-Masuzawa, Taiichiro Seki","doi":"10.1093/bbb/zbaf129","DOIUrl":"https://doi.org/10.1093/bbb/zbaf129","url":null,"abstract":"Inulin, a soluble dietary fiber, is widely recognized for its gut health benefits; however, its role in the progression of ulcerative colitis remains unclear. In this study, we investigated the effects of inulin supplementation on colitis induced by administering dextran sodium sulfate (DSS) to BALB/c mice. Mice were fed diets containing 10% inulin or cellulose, followed by administration of 2% DSS in drinking water. Inulin-supplemented mice exhibited a higher disease activity index and more severe epithelial damage compared to cellulose-fed controls. Similar pathological features were observed in mice administered polyethylene glycol to induce osmotic diarrhea, suggesting that increased luminal osmotic pressure may exacerbate colitis. Notably, co-administration of polycarbophil calcium with inulin ameliorated clinical symptoms and attenuated tissue damage. These findings suggest that inulin may aggravate colitis, potentially through increased luminal osmotic pressure. Therefore, managing osmotic diarrhea may represent a therapeutic strategy to mitigate colitis symptoms associated with certain dietary fibers.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of an endodextranase from Flavobacterium johnsoniae, the first endo-acting enzyme classified within glycoside hydrolase family 27. 强johnsoniae黄杆菌中一个内切葡聚糖酶的鉴定和表征，这是糖苷水解酶家族中第一个内切酶。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-04 DOI: 10.1093/bbb/zbaf130

Shuntaro Nakamura, Kazumi Funane, Takatsugu Miyazaki

{"title":"Identification and characterization of an endodextranase from Flavobacterium johnsoniae, the first endo-acting enzyme classified within glycoside hydrolase family 27.","authors":"Shuntaro Nakamura, Kazumi Funane, Takatsugu Miyazaki","doi":"10.1093/bbb/zbaf130","DOIUrl":"https://doi.org/10.1093/bbb/zbaf130","url":null,"abstract":"Glycoside hydrolase family 27 (GH27) consists primarily of exo-acting enzymes, including α-galactosidase, α-N-acetylgalactosaminidase, β-L-arabinopyranosidase, and isomalto-dextranase. Here, we examined the gene expression profiles and biochemical characteristics of a multi-domain protein, Fjoh_4436 (named FjGH27A), which contains a GH27 catalytic domain and is encoded upstream of the previously described branched dextran utilization locus in Flavobacterium johnsoniae. Expression of this gene increased 7.7-fold when cultured with α-(1→2)- and α-(1→3)-branched dextran, produced by Leuconostoc citreum S-32, as the carbon source, compared with cultures using glucose or linear dextran. The catalytic domain of FjGH27A exhibited hydrolytic activity against linear dextran and produced isomaltooligosaccharides of various sizes in an endo-acting manner. The enzyme showed the highest activity toward linear dextran at pH 5.5-6.0 and 25°C-30°C. This represents the first identification of an endo-acting enzyme in the GH27 family, offering new insights into the mechanism of branched dextran degradation mediated by FjGH27A.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral administration of l-lysine potently promotes GLP-1 secretion and lowers glycemic response in rats. 口服赖氨酸可促进大鼠GLP-1分泌，降低血糖反应。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-09-03 DOI: 10.1093/bbb/zbaf124

Akiho Irie, Tohru Hira

引用次数: 0

Existence, biosynthesis, metabolism, bioactivities, and biological functions of 12-hydroxyjasmonates. 12-羟基茉莉酸盐的存在、生物合成、代谢、生物活性和生物学功能。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-30 DOI: 10.1093/bbb/zbaf127

Naoki Kitaoka

引用次数: 0

Discovery and characterization of high-activity formate oxidase with low Km value under nonacidic conditions. 非酸性条件下低Km值高活性甲酸氧化酶的发现与表征。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf092

Zhenyu Zhai, Yi-Heng P Job Zhang

引用次数: 0

Conferring transfer capability by cloning transfer-related genes from the conjugative linear plasmid SAP1. 从共轭线性质粒SAP1克隆转移相关基因以获得转移能力。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf090

Yuta Fukada, Naoya Maruyama, Shota Endou, Haruo Ikeda, Masakazu Kataoka

引用次数: 0

Suppressive effect of transient receptor potential vanilloid 1 on olfactory receptor signal transduction. 瞬时受体电位香兰素1对嗅觉受体信号转导的抑制作用。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf093

Sakura Moriyama, Shuji Hinuma, Shun'ichi Kuroda

{"title":"Suppressive effect of transient receptor potential vanilloid 1 on olfactory receptor signal transduction.","authors":"Sakura Moriyama, Shuji Hinuma, Shun'ichi Kuroda","doi":"10.1093/bbb/zbaf093","DOIUrl":"10.1093/bbb/zbaf093","url":null,"abstract":"We investigated the effect of transient receptor potential vanilloid 1 (TRPV1) on ligand-stimulated olfactory receptor (OR) activation [intracellular cyclic adenosine monophosphate (cAMP) elevation]. The co-expression of TRPV1 with OR51E1 in HEK293T cells suppressed the activation of OR51E1 stimulated with isovaleric acid. This suppression was further potentiated by capsaicin stimulation. On the other hand, capsaicin suppressed the ligand-induced cAMP production in HEK293T cells expressing OR51E1 without co-transfection of the TRPV1 expression plasmid. Treatment with siRNA for TRPV1 offset this capsaicin's effect on ligand-induced OR51E1 activation, suggesting the involvement of endogenously expressed TRPV1 in this suppression. Additionally, TRPV1-dependent suppression of OR51E1 activation was abolished by Ca2+ depletion in the extracellular fluid. A calcium ionophore (A23187) exhibited effects like TRPV1 on the OR response. Furthermore, the suppressive effect of A23187 was inhibited by a G protein-coupled receptor kinase (GRK) inhibitor, CCG21022. Our results indicate that TRPV1 suppresses OR activation via Ca²⁺ influx, followed by GRK activation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1302-1311"},"PeriodicalIF":1.3,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agrose matrix stiffness mediated neuroprotective effects against H2O2-induced oxidative stress in HT22 spheroids. 脂糖基质硬度介导HT22球体对h2o2诱导的氧化应激的神经保护作用。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf094

Guang-Zhen Jin

引用次数: 0