Bioscience, Biotechnology, and Biochemistry最新文献_第4页

Gene cloning and characterization of N-carbamyl-l-glutamic acid amidohydrolase involved in ergothioneine utilization in Burkholderia sp. HME13. 伯克霍尔德氏菌利用麦角硫因的n -氨基甲酰-l-谷氨酸氨基水解酶基因克隆及特性研究

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-24 DOI: 10.1093/bbb/zbae180

Hisashi Muramatsu, Masaaki Yamada, Hiroki Maguchi, Shin-Ichiro Kato

{"title":"Gene cloning and characterization of N-carbamyl-l-glutamic acid amidohydrolase involved in ergothioneine utilization in Burkholderia sp. HME13.","authors":"Hisashi Muramatsu, Masaaki Yamada, Hiroki Maguchi, Shin-Ichiro Kato","doi":"10.1093/bbb/zbae180","DOIUrl":"10.1093/bbb/zbae180","url":null,"abstract":"Burkholderia sp. HME13 utilizes ergothioneine, a strong antioxidant, as the nitrogen source. We have previously shown that ergothionase, thiourocanate hydratase, 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase, and hydantoin-5-propionic acid amidohydrolase may be involved in ergothioneine utilization in this strain. In this study, we identified the ertE gene in Burkholderia sp. HME13, which encodes a bivalent metal-dependent N-carbamyl-l-glutamic acid amidohydrolase (ErtE). ErtE showed maximum activity at 60 °C and pH 7.0 and was stable at temperatures up to 55 °C and pH 6.5-8.0. The Km and Vmax values of ErtE for N-carbamyl-l-glutamic acid were 0.74 m m and 140 U/mg, respectively. Ethylenediaminetetraacetic acid-treated ErtE showed no enzymatic activity, which was restored upon the addition of Co2+, Mn2+, Ni2+, and Fe2+. Expression analyses and enzymatic assays suggested that ErtE is involved in ergothioneine utilization in this strain. Finally, we propose a mechanism for ergothioneine utilization in Burkholderia sp. HME13.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"255-262"},"PeriodicalIF":1.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Looking back at the achievements of functional food science in Japan. 回顾日本功能食品科学的成就。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-24 DOI: 10.1093/bbb/zbae134

Akira Murakami

引用次数: 0

Rosae Multiflorae Fructus Extracts Regulate the Differentiation and Vascular Endothelial Cell-mediated Proliferation of Keratinocytes.

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-23 DOI: 10.1093/bbb/zbaf007

Sae Asayama, Tami Igarashi, Yosihimi Abe, Ayaka Iwasaki, Mizuki Kubo, Ayaka Ikeda, Kouki Akiyama, Tadashi Okamoto, Masayuki Yagi, Yoko Niki, Hideya Ando, Masamitsu Ichihashi, Ken-Ichi Mizutani

{"title":"Rosae Multiflorae Fructus Extracts Regulate the Differentiation and Vascular Endothelial Cell-mediated Proliferation of Keratinocytes.","authors":"Sae Asayama, Tami Igarashi, Yosihimi Abe, Ayaka Iwasaki, Mizuki Kubo, Ayaka Ikeda, Kouki Akiyama, Tadashi Okamoto, Masayuki Yagi, Yoko Niki, Hideya Ando, Masamitsu Ichihashi, Ken-Ichi Mizutani","doi":"10.1093/bbb/zbaf007","DOIUrl":"https://doi.org/10.1093/bbb/zbaf007","url":null,"abstract":"Keratinocytes are the primary component of the epidermis, so maintaining the precise balance between proliferation and differentiation is essential for conserving epidermal structure and function. Rosae multiflorae fructus extract (RMFE) has wide application in the cosmetic industry, but the molecular mechanisms underlying beneficial effects on keratinocytes are still not fully understood. In this study, we found that RMFE promoted epidermal differentiation and enhanced the barrier function of normal human epidermal keratinocytes (NHEKs) and three-dimensional epidermis model in culture. In addition, RMFE promoted human umbilical vein endothelial cell (HUVEC) proliferation and angiogenesis, whereas the conditioned medium from RMFE-treated HUVECs further promoted NHEK proliferation and increased wound healing ability. Analysis of constituent bioactivities identified a quercetin derivative as a potential mediator of NHEK and HUVEC responses to RMFE. Taken together, these results suggest that RMFE enhances epidermal functions through both direct effects on keratinocytes and indirect effects mediated by endothelial cells.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A unique substrate specificity of PonAAS2, an aromatic aldehyde synthase, involved in a phytohormone auxin biosynthesis in a gall-inducing sawfly Euura sp. "Pontania". PonAAS2是一种芳香醛合成酶，参与植物激素生长素的生物合成，具有独特的底物特异性。“Pontania”。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-16 DOI: 10.1093/bbb/zbaf005

Yoshihito Suzuki, Hikaru Ichikawa, Yuri Kunioka, Umi Miyata, Shugo Nakamura, Zui Fujimoto

引用次数: 0

Regulation of eumelanogenesis-related genes by ethanol extract of Melaleuca Leucadendron leaves. 千层桉叶乙醇提取物对真黑发生相关基因的调控作用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-16 DOI: 10.1093/bbb/zbae204

Myungsam Park, Younhwa Nho, Insanu Muhamad, Rizaldy Defri, Jae Sung Hwang

{"title":"Regulation of eumelanogenesis-related genes by ethanol extract of Melaleuca Leucadendron leaves.","authors":"Myungsam Park, Younhwa Nho, Insanu Muhamad, Rizaldy Defri, Jae Sung Hwang","doi":"10.1093/bbb/zbae204","DOIUrl":"https://doi.org/10.1093/bbb/zbae204","url":null,"abstract":"Eumelanin, a type of skin melanin pigment, possesses the ability to absorb a wide range of wavelengths, providing protection to the skin from ultraviolet radiation. However, excessive production of eumelanin may result in hyperpigmentation. Consequently, the development of skin-brightening products that suppress eumelanin synthesis to achieve a lighter and more even skin tone is necessary. The present study investigated the potential application of Melaleuca leucadendron as a natural skin-brightening agent. We investigated the effects of the ethanol leaf extract of M. Leucadendron on α-melanocyte-stimulating hormone-induced melanogenesis in B16F10 cells and identified the key factors involved in melanogenesis. This leaf extract significantly inhibited melanin production by downregulating the expression of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1, and dopachrome tautomerase without causing cytotoxicity. Furthermore, it substantially reduced melanin content in a 3D skin model. These results suggest that the extract of M. Leucadendron is a promising, safe, and effective skin-brightening agent.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of microemulsion system on water dispersibility and bioavailability of γ-oryzanol. 微乳液体系对γ-谷维醇水分散性和生物利用度的影响。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-15 DOI: 10.1093/bbb/zbaf002

Junya Ito, Naoko Kumagai, Ayaka Suzuki, Naoki Shoji, Isabella Supardi Parida, Mamoru Takahashi, Kiyotaka Nakagawa

引用次数: 0

FRPR-1, a G protein-coupled receptor (GPCR) in the FMRFamide-related peptide receptor family, modulates larval development as a receptor candidate of the FMRFamide-like peptide FLP-1 in Caenorhabditis elegans. FRPR-1是fmrfamily相关肽受体家族中的G蛋白偶联受体（GPCR），在秀丽隐杆线虫中作为fmrfamily样肽FLP-1的候选受体调节幼虫的发育。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-15 DOI: 10.1093/bbb/zbaf004

Risako Une, Riko Uegaki, Sho Maega, Masahiro Ono, Tomohiro Bito, Takashi Iwasaki, Akira Shiraishi, Honoo Satake, Tsuyoshi Kawano

{"title":"FRPR-1, a G protein-coupled receptor (GPCR) in the FMRFamide-related peptide receptor family, modulates larval development as a receptor candidate of the FMRFamide-like peptide FLP-1 in Caenorhabditis elegans.","authors":"Risako Une, Riko Uegaki, Sho Maega, Masahiro Ono, Tomohiro Bito, Takashi Iwasaki, Akira Shiraishi, Honoo Satake, Tsuyoshi Kawano","doi":"10.1093/bbb/zbaf004","DOIUrl":"https://doi.org/10.1093/bbb/zbaf004","url":null,"abstract":"FMRFamide-like peptides (FLPs) and their receptors FMRFamide-related peptide receptors (FRPRs) are widely conserved in free-living and parasitic nematodes. Herein, we identified FRPR-1 as a of FLP-1 receptor candidate involved in larval development and diapause in the model nematode Caenorhabditis elegans. Our molecular genetic study, supported by in silico research, revealed the following: 1) frpr-1 loss-of-function completely suppresses the promotion of larval diapause caused by flp-1 overexpression; 2) AlphaFold2 analysis revealed the binding of FLP-1 to FRPR-1; 3) FRPR-1 as well as FLP-1modulates the production and secretion of the predominant insulin-like peptide DAF-28, which is produced in ASI neurons; and 4) the suppression of larval diapause by frpr-1 loss-of-function is completely suppressed by a daf-28 defect. Thus, FRPR-1 regulates larval development and diapause by modulating DAF-28 production and secretion. This study may provide new insights into the development of novel nematicides targeting parasitic nematodes using FRPR-1 inhibitors.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional expression mechanisms of food-derived components based on target proteins. 基于靶蛋白的食源性成分功能表达机制。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-13 DOI: 10.1093/bbb/zbaf003

Takakazu Mitani

引用次数: 0

Tetragonia tetragonioides extract prevented high-fat diet-induced obesity and changed hepatic and adipose transcriptomic signatures in C57BL/6J male mice. 四角草提取物可预防高脂肪饮食引起的肥胖，并改变C57BL/6J雄性小鼠的肝脏和脂肪转录组特征。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-11 DOI: 10.1093/bbb/zbaf001

Hea Ry Oh, Yong Hyun Park, Hye Ryeong Hong, Hyun Jin Kim, Jinbong Park, Yohan Han, Seong-Gyu Ko, Eui Cheol Shin, Tae Gyun Kim, Hyung Taek Cho, Jeong Hoon Pan, Hyo Ri Shin, Youn Young Shim, Martin J T Reaney, Tae Jin Cho, Ji Youn Hong, Young Jun Kim, Bok Kyung Han, Geung-Joo Lee, Kangwook Lee, Seon Gil Do, Jae Kyeom Kim

{"title":"Tetragonia tetragonioides extract prevented high-fat diet-induced obesity and changed hepatic and adipose transcriptomic signatures in C57BL/6J male mice.","authors":"Hea Ry Oh, Yong Hyun Park, Hye Ryeong Hong, Hyun Jin Kim, Jinbong Park, Yohan Han, Seong-Gyu Ko, Eui Cheol Shin, Tae Gyun Kim, Hyung Taek Cho, Jeong Hoon Pan, Hyo Ri Shin, Youn Young Shim, Martin J T Reaney, Tae Jin Cho, Ji Youn Hong, Young Jun Kim, Bok Kyung Han, Geung-Joo Lee, Kangwook Lee, Seon Gil Do, Jae Kyeom Kim","doi":"10.1093/bbb/zbaf001","DOIUrl":"https://doi.org/10.1093/bbb/zbaf001","url":null,"abstract":"Obesity, often driven by high-fat diets (HFD), is a major global health issue, necessitating effective preventive measures. Tetragonia tetragonoides, a plant with known medicinal properties, has not been extensively studied for its effects on HFD-induced obesity and related genetic changes in mice. This study explores the impact of Tetragonia tetragonoides extract (TTE; 300 mg/kg) on obesity-related traits in C57BL/6J male mice, with a focus on transcriptomic changes in the liver and white adipose tissue (WAT). Over eight weeks, TTE supplementation led to significant reductions in obesity-related phenotypes and modulated gene expression altered by HFD. Key genes like Cd180 and MUPs, linked to immune responses and lipid metabolism, were notably influenced by TTE. The study highlighted TTE's effects on lipid metabolism pathways in the liver and immune processes in WAT, underscoring its potential as an anti-obesity agent, while advocating for further research into its bioactive components.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmodium falciparum histidine-rich protein 2 exhibits cell penetration and cytotoxicity with autophagy dysfunction. 恶性疟原虫富组氨酸蛋白2表现出细胞渗透和细胞毒性，并伴有自噬功能障碍。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-01-07 DOI: 10.1093/bbb/zbae209

Takashi Iwasaki, Mayu Shimoda, Haru Kanayama, Tsuyoshi Kawano

引用次数: 0