Suppressive Effect of Transient Receptor Potential Vanilloid 1 on Olfactory Receptor Signal Transduction.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-06-23 DOI:10.1093/bbb/zbaf093

Sakura Moriyama, Shuji Hinuma, Shun'ichi Kuroda

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Abstract

We investigated the effect of transient receptor potential vanilloid 1 (TRPV1) on ligand-stimulated olfactory receptor (OR) activation (intracellular cAMP elevation). The co-expression of TRPV1 with OR51E1 in HEK293T cells suppressed the activation of OR51E1 stimulated with isovaleric acid. This suppression was further potentiated by capsaicin stimulation. On the other hand, capsaicin suppressed the ligand-induced cAMP production in HEK293T cells expressing OR51E1 without co-transfection of the TRPV1 expression plasmid. Treatment with siRNA for TRPV1 offset this capsaicin's effect on ligand-induced OR51E1 activation, suggesting the involvement of endogenously expressed TRPV1 in this suppression. Additionally, TRPV1-dependent suppression of OR51E1 activation was abolished by Ca2+ depletion in the extracellular fluid. A calcium ionophore (A23187) exhibited effects like TRPV1 on the OR response. Furthermore, the suppressive effect of A23187 was inhibited by a G protein-coupled receptor kinase (GRK) inhibitor CCG21022. Our results indicate that TRPV1 suppresses OR activation via Ca²⁺ influx, followed by GRK activation.

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瞬时受体电位香兰素1对嗅觉受体信号转导的抑制作用。

我们研究了瞬时受体电位香草素1 （TRPV1）对配体刺激嗅觉受体（OR）激活（细胞内cAMP升高）的影响。TRPV1与OR51E1在HEK293T细胞中的共表达抑制了异戊酸刺激下OR51E1的活化。辣椒素的刺激进一步增强了这种抑制作用。另一方面，在不共转染TRPV1表达质粒的情况下，辣椒素抑制了表达OR51E1的HEK293T细胞中配体诱导的cAMP产生。用siRNA处理TRPV1抵消了辣椒素对配体诱导的OR51E1激活的影响，表明内源性表达的TRPV1参与了这种抑制。此外，trpv1依赖性的OR51E1激活抑制被细胞外液中的Ca2+耗尽所消除。钙离子载体（A23187）表现出与TRPV1类似的效应。此外，G蛋白偶联受体激酶（GRK）抑制剂CCG21022可抑制A23187的抑制作用。我们的研究结果表明，TRPV1通过Ca 2 +内流抑制OR激活，随后抑制GRK激活。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).