用分子筛脱氢法制备coelenterazine。

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mitsuhiro Nakamura, Kazuya Nakayama, Kazuo Matsuda, Satoshi Inouye
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引用次数: 0

摘要

Coelenterazine (CTZ)和脱氢Coelenterazine (dCTZ, CTZ的脱氢形式)首次在发光鱿鱼(Watasenia scintillans)的肝脏中被发现。在本文中,我们首次证明了CTZ可以在异丙醇中使用分子筛(MS4A/Na+, MS13X/Na+, MS5A/Ca2+和MS3A/K+)在37°C下成功转化为dCTZ。到dCTZ的转化效率依次为:MS4A/Na+ = MS13X/Na+ > MS5A/Ca2+ > MS3A/K+。改性的MS4A (TMS-MS4A、NH4+-MS4A和H+-MS4A)的效率较低,说明分子筛中的阳离子在CTZ脱氢反应中起关键作用。值得注意的是,从反应混合物中回收的MS4A可以重复使用,无需热再生脱水。在纯化的dCTZ中,使用荧光素-荧光素酶反应没有检测到残留的CTZ, dCTZ可以作为发光试验的底物来测定酶转化为CTZ的活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dehydrogenation of coelenterazine to dehydrocoelenterazine using molecular sieves.

Coelenterazine (CTZ) and dehydrocoelenterazine (dCTZ, a dehydrogenated form of CTZ) were first identified in the liver of the luminescent squid Watasenia scintillans. In this report, we demonstrate for the first time that CTZ can be successfully converted to dCTZ using molecular sieves (MS4A/Na+, MS13X/Na+, MS5A/Ca2+, and MS3A/K+) in isopropyl alcohol at 37 °C for 3 h. The conversion efficiency to dCTZ followed the order: MS4A/Na+ = MS13X/Na+ > MS5A/Ca2+ > MS3A/K+. Lower efficiencies observed with modified MS4A (TMS-MS4A, NH4+-MS4A, and H+-MS4A) suggest that cations in the molecular sieves play a crucial role in the dehydrogenation reaction of CTZ. Notably, MS4A recovered from the reaction mixture could be reused without thermal regeneration for dehydration. In purified dCTZ, no residual CTZ was detected using the luciferin-luciferase reaction, and dCTZ can be used as a substrate in luminescence assays to determine enzymatic conversion activity to CTZ.

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来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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