{"title":"用分子筛脱氢法制备coelenterazine。","authors":"Mitsuhiro Nakamura, Kazuya Nakayama, Kazuo Matsuda, Satoshi Inouye","doi":"10.1093/bbb/zbaf109","DOIUrl":null,"url":null,"abstract":"<p><p>Coelenterazine (CTZ) and dehydrocoelenterazine (dCTZ, a dehydrogenated form of CTZ) were first identified in the liver of the luminescent squid Watasenia scintillans. In this report, we demonstrate for the first time that CTZ can be successfully converted to dCTZ using molecular sieves (MS4A/Na+, MS13X/Na+, MS5A/Ca2+, and MS3A/K+) in isopropyl alcohol at 37 °C for 3 h. The conversion efficiency to dCTZ followed the order: MS4A/Na+ = MS13X/Na+ > MS5A/Ca2+ > MS3A/K+. Lower efficiencies observed with modified MS4A (TMS-MS4A, NH4+-MS4A, and H+-MS4A) suggest that cations in the molecular sieves play a crucial role in the dehydrogenation reaction of CTZ. Notably, MS4A recovered from the reaction mixture could be reused without thermal regeneration for dehydration. In purified dCTZ, no residual CTZ was detected using the luciferin-luciferase reaction, and dCTZ can be used as a substrate in luminescence assays to determine enzymatic conversion activity to CTZ.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1425-1431"},"PeriodicalIF":1.3000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dehydrogenation of coelenterazine to dehydrocoelenterazine using molecular sieves.\",\"authors\":\"Mitsuhiro Nakamura, Kazuya Nakayama, Kazuo Matsuda, Satoshi Inouye\",\"doi\":\"10.1093/bbb/zbaf109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Coelenterazine (CTZ) and dehydrocoelenterazine (dCTZ, a dehydrogenated form of CTZ) were first identified in the liver of the luminescent squid Watasenia scintillans. In this report, we demonstrate for the first time that CTZ can be successfully converted to dCTZ using molecular sieves (MS4A/Na+, MS13X/Na+, MS5A/Ca2+, and MS3A/K+) in isopropyl alcohol at 37 °C for 3 h. The conversion efficiency to dCTZ followed the order: MS4A/Na+ = MS13X/Na+ > MS5A/Ca2+ > MS3A/K+. Lower efficiencies observed with modified MS4A (TMS-MS4A, NH4+-MS4A, and H+-MS4A) suggest that cations in the molecular sieves play a crucial role in the dehydrogenation reaction of CTZ. Notably, MS4A recovered from the reaction mixture could be reused without thermal regeneration for dehydration. In purified dCTZ, no residual CTZ was detected using the luciferin-luciferase reaction, and dCTZ can be used as a substrate in luminescence assays to determine enzymatic conversion activity to CTZ.</p>\",\"PeriodicalId\":9175,\"journal\":{\"name\":\"Bioscience, Biotechnology, and Biochemistry\",\"volume\":\" \",\"pages\":\"1425-1431\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience, Biotechnology, and Biochemistry\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1093/bbb/zbaf109\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience, Biotechnology, and Biochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/bbb/zbaf109","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Dehydrogenation of coelenterazine to dehydrocoelenterazine using molecular sieves.
Coelenterazine (CTZ) and dehydrocoelenterazine (dCTZ, a dehydrogenated form of CTZ) were first identified in the liver of the luminescent squid Watasenia scintillans. In this report, we demonstrate for the first time that CTZ can be successfully converted to dCTZ using molecular sieves (MS4A/Na+, MS13X/Na+, MS5A/Ca2+, and MS3A/K+) in isopropyl alcohol at 37 °C for 3 h. The conversion efficiency to dCTZ followed the order: MS4A/Na+ = MS13X/Na+ > MS5A/Ca2+ > MS3A/K+. Lower efficiencies observed with modified MS4A (TMS-MS4A, NH4+-MS4A, and H+-MS4A) suggest that cations in the molecular sieves play a crucial role in the dehydrogenation reaction of CTZ. Notably, MS4A recovered from the reaction mixture could be reused without thermal regeneration for dehydration. In purified dCTZ, no residual CTZ was detected using the luciferin-luciferase reaction, and dCTZ can be used as a substrate in luminescence assays to determine enzymatic conversion activity to CTZ.
期刊介绍:
Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).