Podophyllotoxin and α-peltatin inhibit nuclear factor κB activation and gene expression stimulated by a double-stranded RNA analogue in human umbilical vein endothelial cells.

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuka Yokota, Piimwara Yarangsee, Kosuke Baba, Saki Sasaki, Hiroyuki Hirano, Hiroyuki Osada, Takao Kataoka
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引用次数: 0

Abstract

In vascular endothelial cells, proinflammatory cytokines and Toll-like receptor (TLR) ligands up-regulate the expression of adhesion molecules. In the present study, screening of the RIKEN Natural Products Depository chemical libraries identified podophyllotoxin and α-peltatin, which inhibited intercellular adhesion molecule-1 (ICAM-1) expression induced by polyinosinic-polycytidylic acid (Poly(I:C)) as a TLR3 ligand. In human umbilical vein endothelial cells (HUVEC), podophyllotoxin and its derivative α-peltatin inhibited Poly(I:C)-induced increases in the mRNA expression of ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. In addition, podophyllotoxin and α-peltatin inhibited the Poly(I:C)-induced nuclear translocation of the nuclear factor κB (NF-κB) subunit RelA. Microtubule-targeting agents (colchicine, vinblastine, and paclitaxel) exerted different effects on the Poly(I:C)-induced mRNA expression of ICAM-1, VCAM-1, and E-selectin. Vinblastine potently inhibited nuclear RelA translocation in Poly(I:C)-stimulated HUVEC, whereas colchicine and paclitaxel did not. Collectively, these results demonstrate that podophyllotoxin and α-peltatin inhibited NF-κB activation and mRNA expression by TLR3 stimulation in HUVEC.

足臼毒素和α-peltatin抑制双链RNA类似物刺激的人脐静脉内皮细胞核因子κB的激活和基因表达。
在血管内皮细胞中,促炎细胞因子和toll样受体(TLR)配体上调黏附分子的表达。在本研究中,通过筛选RIKEN天然产物库化学文库,鉴定出podophyllotoxin和α-peltatin作为TLR3配体抑制多肌苷-多胞酸(Poly(I: C))诱导的细胞间粘附分子-1 (ICAM-1)的表达。在人脐静脉内皮细胞(HUVEC)中,鬼臼毒素及其衍生物α-peltatin抑制Poly(I: C)诱导的ICAM-1、血管细胞粘附分子-1 (VCAM-1)和e-选择素mRNA表达的增加。此外,鬼臼毒素和α-peltatin抑制Poly(I: C)诱导的核因子κB (NF-κB)亚基RelA的核易位。微管靶向药物(秋水仙碱、长春花碱和紫杉醇)对Poly(I: C)诱导的ICAM-1、VCAM-1和e-选择素的mRNA表达有不同的影响。长春花碱能有效抑制Poly(I: C)刺激的HUVEC的核RelA易位,而秋水秋碱和紫杉醇则没有作用。总之,这些结果表明,鬼臼毒素和α-peltatin通过刺激TLR3抑制HUVEC中NF-κB的激活和mRNA的表达。
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来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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