直接向酿酒酵母传递蛋白质的合成肽的评价。

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Shota Isogai, Ryoya Tanahashi, Hiroshi Takagi, Akira Nishimura
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引用次数: 0

摘要

由于酿酒酵母细胞壁坚硬,将功能蛋白有效地传递到酵母中仍然是一个重大的技术挑战。在这里,我们报告了合成肽标签,特别是穿透素,尽管具有实质性的细胞毒性,但可以几乎完全递送GFP。该方法为酵母系统中蛋白质的细胞内传递和功能分析提供了一个有前途的非遗传平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of synthetic peptides for direct protein delivery into Saccharomyces cerevisiae.

Efficient intracellular delivery of functional proteins into the yeast Saccharomyces cerevisiae remains a major technical challenge due to its rigid cell wall. Here, we report that synthetic peptide tags, particularly penetratin, enable nearly complete delivery of GFP, albeit with substantial cytotoxicity. This method provides a promising non-genetic platform for the intracellular delivery and functional analysis of proteins in yeast systems.

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来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
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