Bioscience, Biotechnology, and Biochemistry最新文献_第7页

TRIM59 regulates autophagy-dependent ferroptosis in non-small cell lung cancer by modulating the ubiquitination of TMEM164. TRIM59通过调节TMEM164的泛素化调节非小细胞肺癌自噬依赖性铁凋亡。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-06 DOI: 10.1093/bbb/zbaf118

Jinpeng Qiao, Kai Chen

引用次数: 0

Improved transformation system of Bacillus subtilis competent cells using PCR DNA fragments as donor DNA. 改良的枯草芽孢杆菌能态细胞PCR DNA片段供体转化体系。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-05 DOI: 10.1093/bbb/zbaf120

Kazuhisa Sawada, Keiji Endo, Masatoshi Tohata, Katsuya Ozaki, Masakazu Kataoka

引用次数: 0

Synthesis of Sialylgalactose Analogs via Fully α-Selective Sialylation. 全α-选择性唾液酰化合成唾液酰半乳糖类似物。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-04 DOI: 10.1093/bbb/zbaf117

Asuka Ogawa, Naoko Komura, Hide-Nori Tanaka, Akihiro Imamura, Hideharu Ishida, Hiromune Ando

引用次数: 0

Hirudin Alleviates Renal Fibrosis by Inducing Autophagy to Suppress NLRP3 Inflammasome Activation. 水蛭素通过诱导自噬抑制NLRP3炎性体激活减轻肾纤维化。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-07-29 DOI: 10.1093/bbb/zbaf114

Chunli Long, Fang Lan, Hui Xie, Jiefang Chen, Yongxiang Xie

{"title":"Hirudin Alleviates Renal Fibrosis by Inducing Autophagy to Suppress NLRP3 Inflammasome Activation.","authors":"Chunli Long, Fang Lan, Hui Xie, Jiefang Chen, Yongxiang Xie","doi":"10.1093/bbb/zbaf114","DOIUrl":"https://doi.org/10.1093/bbb/zbaf114","url":null,"abstract":"Renal fibrosis is a pathological feature of chronic kidney injury that contributes to renal failure. This study aimed to explore the effect of Hirudin on renal fibrosis. The anti-fibrotic effect of Hirudin was evaluated using unilateral ureteral obstruction (UUO) rats and TGF-β-treated HK-2 cells. The autophagy inhibitor 3-Methyladenine was used to further explore the potential mechanism. Hirudin treatment significantly reduced UUO-induced elevations in blood urea nitrogen, creatinine, and alpha-smooth muscle actin (α-SMA) levels, and improved kidney injury and renal fibrosis. In addition, Hirudin markedly decreased NLRP3 inflammasome-related protein expression and increased autophagy-related protein expression in the kidneys of UUO rats. Hirudin significantly increased cell viability, reduced α-SMA and NLRP3 inflammasome-related protein levels, and increased autophagy-related protein levels in TGF-β-treated HK-2 cells. However, the effects of Hirudin were counteracted by 3-Methyladenine. In conclusion, Hirudin inhibits the activation of NLRP3 inflammasome by inducing autophagy to improve renal fibrosis.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inactivation of bglH, a key β-glucosidase gene involved in isoflavone aglycone production, in Bacillus subtilis strain Miyagino. 枯草芽孢杆菌Miyagino菌株中参与异黄酮苷元生产的β-葡萄糖苷酶关键基因bglH失活。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-07-26 DOI: 10.1093/bbb/zbaf115

Shyuichiro Inagaki

引用次数: 0

Purification and characterization of CcdB and CcdA toxin-antitoxin system from Acetobacter malorum. 甘露醋杆菌CcdB和CcdA毒素-抗毒素系统的纯化及特性研究。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-07-23 DOI: 10.1093/bbb/zbaf063

Chenguang Yang, Zhenhua Fan, Lvming Wu, Bo Fu, Hongbin Sun

引用次数: 0

Conformational gating in CYP154C2: Gln230-mediated substrate recognition and catalytic switching revealed by structural dynamics. CYP154C2的构象门控：结构动力学揭示的gln230介导的底物识别和催化开关。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-07-23 DOI: 10.1093/bbb/zbaf076

Jian Yang, Jiekun Huang, Xinghan He, Shinya Fushinobu, Lian-Hua Xu

{"title":"Conformational gating in CYP154C2: Gln230-mediated substrate recognition and catalytic switching revealed by structural dynamics.","authors":"Jian Yang, Jiekun Huang, Xinghan He, Shinya Fushinobu, Lian-Hua Xu","doi":"10.1093/bbb/zbaf076","DOIUrl":"10.1093/bbb/zbaf076","url":null,"abstract":"Previously, we reported that CYP154C2 from Streptomyces avermitilis is capable of catalyzing the 2α-hydroxylation of the two model substrates, testosterone (TES) and androstenedione (ASD), and resolved the closed structures of both the substrate-free form and the TES-bound form. In this study, we extend these findings by determining the open-conformation structures of the substrate-free and ASD-bound forms-a rare achievement among bacterial P450s. Structural analyses revealed coordinated conformational shifts in the FG helices, HI helices, and BC loop during open-to-closed transitions. Despite divergent overall conformations, both substrates positioned their C2 atoms near the heme iron, aligning for 2α-hydroxylation. Mutagenesis studies established Gln230's pivotal role in substrate recognition and catalytic activation. High-resolution crystallography (1.97 Å) of the Q230A mutant revealed polyethylene glycol-occupied catalytic pockets (indicating complete loss of TES binding) while maintaining the open conformation. These results provide atomic-level evidence that Gln230 coordinates both substrate-driven conformational gating and catalytic site optimization.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1144-1153"},"PeriodicalIF":1.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial biotransformation of proteins into amino acids in unpolished Thai and polished Japanese rice varieties cultivated with distinct industrial strains of koji mold. 用不同工业菌种栽培的泰国和日本未抛光水稻品种中蛋白质向氨基酸的微生物转化。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-07-23 DOI: 10.1093/bbb/zbaf079

Jirayu Jitpakdee, Kazunari Ito, Yuka Tanino, Hayato Takeuchi, Hideyuki Yamashita, Takuro Nakagawa, Teruhiko Nitoda, Hiroshi Kanzaki

{"title":"Microbial biotransformation of proteins into amino acids in unpolished Thai and polished Japanese rice varieties cultivated with distinct industrial strains of koji mold.","authors":"Jirayu Jitpakdee, Kazunari Ito, Yuka Tanino, Hayato Takeuchi, Hideyuki Yamashita, Takuro Nakagawa, Teruhiko Nitoda, Hiroshi Kanzaki","doi":"10.1093/bbb/zbaf079","DOIUrl":"10.1093/bbb/zbaf079","url":null,"abstract":"We previously reported the cultivation of industrial koji mold strains to produce unpolished Thai-colored rice kojis. These kojis, along with those made from unpolished Thai white rice and polished Japanese white rice, showed increased polyphenol content after cultivation, with the highest levels observed in unpolished Thai-colored rice kojis. In this study, an increase in both proteinogenic and non-proteinogenic amino acid contents, particularly γ-aminobutyric acid (GABA) content, was observed in both unpolished Thai and polished Japanese rice kojis, suggesting the ability of koji mold in the biotransformation of proteins. This increase was almost comparable even when using different rice varieties; in contrast, it varied depending on the koji mold strain used. The observed increase in both polyphenol and functional amino acid contents, especially GABA content, highlights the potential of unpolished Thai and polished Japanese rice kojis, particularly unpolished Thai-colored rice koji, as multifunctional materials, benefiting from polyphenol and amino acid functionalities.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1217-1226"},"PeriodicalIF":1.4,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144156747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liraglutide enhances myotube differentiation and muscle contractile activity upon electric pulse stimulation in mouse skeletal muscle cells. 利拉鲁肽增强电脉冲刺激小鼠骨骼肌细胞的肌管分化和肌肉收缩活性。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-07-23 DOI: 10.1093/bbb/zbaf060

Risa Mukai, Ayumu Kojima, Mizuki Morisasa, Wakako Tawara, Tsukasa Mori, Naoko Goto-Inoue

引用次数: 0

Effects of combination of krill oil with curcumin and/or silymarin on mouse non-alcoholic fatty liver disease. 磷虾油与姜黄素和/或水飞蓟素联合使用对小鼠非酒精性脂肪肝的影响。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-07-23 DOI: 10.1093/bbb/zbaf064

Sidan Lu, Yangyang Jiang, Yong Ling, Xuguang Zhai, Yajun Zhou

引用次数: 0