Bioscience, Biotechnology, and Biochemistry最新文献_第6页

A type II toxin-antitoxin system, ECs3274-ECs3275, in enterohemorrhagic Escherichia coli O157. 肠出血性大肠杆菌 O157 中的 II 型毒素-抗毒素系统 ECs3274-Ecs3275。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae146

Yuka Sasaki, Yuna Mogi, Mizuki Yoshioka, Ke Liu, Yuichi Otsuka

引用次数: 0

Advances in recombinant protein production in microorganisms and functional peptide tags. 微生物重组蛋白生产和功能肽标签方面的进展。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae147

Teruyo Ojima-Kato

引用次数: 0

Construction of a genome-editing system for the thermophilic actinomycete Streptomyces thermodiastaticus K5 strain. 构建嗜热放线菌链霉菌 K5 株的基因组编辑系统。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae157

Kenji Yamagishi, Masakazu Ike, Ken Tokuyasu

引用次数: 0

Eugenol and basil essential oil as priming agents for enhancing Arabidopsis immune response. 丁香酚和罗勒精油是增强拟南芥免疫反应的启动剂。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae156

Shogo Hirose, Soyoka Horiyama, Atsushi Morikami, Kazuki Fujiwara, Hironaka Tsukagoshi

{"title":"Eugenol and basil essential oil as priming agents for enhancing Arabidopsis immune response.","authors":"Shogo Hirose, Soyoka Horiyama, Atsushi Morikami, Kazuki Fujiwara, Hironaka Tsukagoshi","doi":"10.1093/bbb/zbae156","DOIUrl":"10.1093/bbb/zbae156","url":null,"abstract":"Plants, as sessile organisms, must adapt to environmental changes and defend themselves against biotic stress, including pathogen attack. Their immune responses entail recognition of pathogen patterns, activation of defense mechanisms, and accumulation of various antimicrobial compounds. Eugenol, abundant in basil, has antibacterial properties and enhances plant resistance to viruses. However, its priming effects on biotrophic pathogens remain unclear. Thus, we investigated whether eugenol and basil essential oils could prime Arabidopsis thaliana immunity against the hemi-biotroph Pseudomonas syringae pv. maculicola (Psm) MAFF302723. Our study revealed that both eugenol and basil essential oils functioned as priming agents, mitigating disease symptoms upon Psm infection. This priming effect occurred via NPR1-dependent but salicylic acid-independent signaling. Moreover, our gene expression analysis suggested that priming might influence jasmonic acid/ethylene signaling. These findings underscore the potential of employing natural compounds such as basil essential oil to bolster plant immune responses in sustainable agricultural practices.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"41-50"},"PeriodicalIF":1.4,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Choline chloride and N-allylglycine promote plant growth by increasing the efficiency of photosynthesis. 氯化胆碱和 N-烯丙基甘氨酸可提高光合作用的效率，从而促进植物生长。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae145

Naoki Ikeda, Mayu Kamimura, Kousaku Uesugi, Takeshi Kobayashi, Fang-Sik Che

{"title":"Choline chloride and N-allylglycine promote plant growth by increasing the efficiency of photosynthesis.","authors":"Naoki Ikeda, Mayu Kamimura, Kousaku Uesugi, Takeshi Kobayashi, Fang-Sik Che","doi":"10.1093/bbb/zbae145","DOIUrl":"10.1093/bbb/zbae145","url":null,"abstract":"We previously reported that choline chloride and N-allylglycine stimulate photosynthesis in wheat protoplasts. Treatment of Arabidopsis thaliana and Brassica rapa plants with both compounds promoted growth and photosynthesis. To clarify the relationship between the enhancement of photosynthesis and increased growth, A. thaliana T87 cells, which show photosynthesis-dependent growth, and YG1 cells, which use sugar in the medium for growth, were treated with choline chloride or N-allylglycine. Only the T87 cells showed increased growth, suggesting that choline chloride and N-allylglycine promote growth by increasing photosynthetic activity. Transcriptome analysis using choline chloride- and N-allylglycine-treated plants showed that the most abundant transcripts corresponded to photosynthetic electron transfer-related genes among the genes upregulated by both compounds. Furthermore, the compounds also upregulate genes encoding transcription factors that may control the expression of these photosynthetic genes. These results suggest that choline chloride and N-allylglycine promote photosynthesis through increased expression of photosynthetic electron transfer-related genes.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"51-61"},"PeriodicalIF":1.4,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of a S-adenosylmethionine-insensitive methylenetetrahydrofolate reductase identified in methionine-accumulating yeast mutants. 从蛋氨酸积累酵母突变体中发现的对 S-腺苷蛋氨酸不敏感的亚甲基四氢叶酸还原酶的功能分析

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae154

Shota Isogai, Akira Nishimura, Akiko Inoue, Shino Sonohara, Takashi Tsugukuni, Hiroshi Takagi

{"title":"Functional analysis of a S-adenosylmethionine-insensitive methylenetetrahydrofolate reductase identified in methionine-accumulating yeast mutants.","authors":"Shota Isogai, Akira Nishimura, Akiko Inoue, Shino Sonohara, Takashi Tsugukuni, Hiroshi Takagi","doi":"10.1093/bbb/zbae154","DOIUrl":"10.1093/bbb/zbae154","url":null,"abstract":"Essential amino acids (EAAs) are important for the maintenance of brain functions. Therefore, the yeast Saccharomyces cerevisiae that accumulates EAAs would help elderly people ingest appropriate levels of EAAs, which in turn could slow neurodegeneration, extend the healthy lifespan, and improve quality of life. Here, we isolated 2 mutant strains, ETH-80 and ETH-129, that accumulate the EAA methionine. Both strains were derived from a diploid laboratory yeast by conventional mutagenesis and carry a novel mutation in the MET13 gene, which encodes the Ser443Phe variant of methylenetetrahydrofolate reductase. Enzymatic analysis revealed that the Ser443Phe substitution abolished the sensitivity to S-adenosyl methionine (SAM)-mediated inhibition even in the presence of 2 m m SAM, while increasing the activity for NADPH-dependent reduction. Furthermore, yeast cells expressing the Ser443Phe variant showed a 4-fold increase in intracellular methionine content compared to the wild-type Met13. These findings will be useful for the future development of methionine-accumulating yeast strains.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"124-132"},"PeriodicalIF":1.4,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustained germination-promoting effect of cold atmospheric plasma on spinach seeds. 冷大气等离子体对菠菜种子的持续发芽促进作用。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae155

Seiya Nishino, Kaori Shimizu, Fuko Horie, Shizu Fukuda, Shingo Izawa

引用次数: 0

TLR4 promotes smooth muscle cell-derived foam cells formation by inducing receptor-independent macropinocytosis. TLR4 通过诱导与受体无关的大吞噬作用促进平滑肌细胞衍生泡沫细胞的形成。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae153

Xue Chen, Yulai Kang, Chunhua Tang, Lili Zhang, Lu Guo

{"title":"TLR4 promotes smooth muscle cell-derived foam cells formation by inducing receptor-independent macropinocytosis.","authors":"Xue Chen, Yulai Kang, Chunhua Tang, Lili Zhang, Lu Guo","doi":"10.1093/bbb/zbae153","DOIUrl":"10.1093/bbb/zbae153","url":null,"abstract":"Foam cells are primarily formed through scavenger receptors that mediate the uptake of various modified low-density lipoproteins (LDL) into cells. In addition to the receptor-dependent pathway, macropinocytosis is an essential nonreceptor endocytic pathway for vascular smooth muscle cells (VSMCs) to take up lipids. However, the molecular mechanisms underlying this process remain unclear. Primary cultured VSMCs were stimulated with 200 ng/mL lipopolysaccharide (LPS) and 200 µg/mL native LDL (nLDL). We observed a significant increase in Toll-like receptor 4 (TLR4) protein expression and a significant activation of macropinocytosis, which correlated with the highest uptake of nLDL and intracellular lipid deposition in WT VSMCs. However, macropinocytosis was inhibited and lipid accumulation decreased after treatment with macropinocytosis inhibitors and Syk inhibitors in WT VSMCs. Consistently, TLR4 knockout significantly suppressed macropinocytosis and lipid droplets accumulation in VSMCs. Taken together, our findings suggest a critical role of TLR4/Syk signaling in promoting receptor-independent macropinocytosis leading to VSMC-derived foam cells formation.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"22-32"},"PeriodicalIF":1.4,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural insights into the inhibition mechanism of glucosidase inhibitors toward kojibiose hydrolase belonging to glycoside hydrolase family 65. 糖苷酶抑制剂对属于糖苷水解酶家族 65 的柯西比糖水解酶的抑制机制的结构性见解。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae158

Shuntaro Nakamura, Takatsugu Miyazaki

引用次数: 0

Prokaryotic ABCF proteins in overcoming ribosomal stalling: mechanisms, evolution, and perspective for applications in Bio-manufacturing. 原核ABCF蛋白克服核糖体失速：机制、进化和在生物制造中的应用前景。

IF 1.4 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-12-23 DOI: 10.1093/bbb/zbae201

Hiraku Takada, Ryota Sugimoto, Taku Oshima

引用次数: 0