Bioscience, Biotechnology, and Biochemistry最新文献_第6页

Efficient production of d-sedoheptulose using transketolase from Thermus thermophilus HB8 and epimerization reaction using d-tagatose 3-epimerase from Pseudomonas cichorii ST24. 利用嗜热热菌HB8的转酮醇酶和池氏假单胞菌ST24的d -塔格糖3-外聚酶进行高效产d -糖庚糖的反应。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf088

Kana Watanuki, Susumu Mochizuki, Yusuke Hanaki, Hiromi Yoshida, Shigehiro Kamitori, Ken Izumori, Akihide Yoshihara

{"title":"Efficient production of d-sedoheptulose using transketolase from Thermus thermophilus HB8 and epimerization reaction using d-tagatose 3-epimerase from Pseudomonas cichorii ST24.","authors":"Kana Watanuki, Susumu Mochizuki, Yusuke Hanaki, Hiromi Yoshida, Shigehiro Kamitori, Ken Izumori, Akihide Yoshihara","doi":"10.1093/bbb/zbaf088","DOIUrl":"10.1093/bbb/zbaf088","url":null,"abstract":"Heptose is a rare monosaccharide with 7 carbon atoms. In this study, we demonstrated that 2 heptoses can be produced cost-effectively and efficiently using transketolase from Thermus thermophilus HB8 (TtTK). First, alternative substrates to a conventional donor substrate, lithium hydroxypyruvate, were investigated, suggesting that 5-keto-d-fructose (5-KF) was the best donor substrate among the 14 donor substrates evaluated. Using 5-KF and aldopentoses as acceptors, we confirmed that 4 heptoses could be produced, and that the optimal reaction temperature and donor and acceptor substrate concentration are 50 °C and 500 m m, respectively. Furthermore, the d-sedoheptulose produced by the partially purified TtTK was converted to d-allopeptulose through the epimerization by d-tagatose 3-epimerase (d-TE) from Pseudomonas cichorii ST24. The equilibrium ratio of the epimerization reaction between d-sedoheptulose and D-alloheptulose was 45:55. This is the first report that d-TE could catalyze an epimerization reaction between d-sedoheptulose and d-alloheptulose.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1279-1285"},"PeriodicalIF":1.3,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic resource and hormonal profiling of the smut fungus, Ustilago esculenta, coexisting with Zizania latifolia and forming Makomotake. 黑穗病菌黑穗病菌（Ustilago esculenta）与紫穗病菌（Zizania latifolia）共存并形成Makomotake的转录组资源和激素分析。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf084

Yuki Tanaka, Akiko Ono, Daichi Aoki, Rina Matsuda, Yuina Kitsukawa, Jae-Hoon Choi, Xiaonan Xie, M Catherine Aime, Hirokazu Kawagishi, Tomohiro Suzuki

{"title":"Transcriptomic resource and hormonal profiling of the smut fungus, Ustilago esculenta, coexisting with Zizania latifolia and forming Makomotake.","authors":"Yuki Tanaka, Akiko Ono, Daichi Aoki, Rina Matsuda, Yuina Kitsukawa, Jae-Hoon Choi, Xiaonan Xie, M Catherine Aime, Hirokazu Kawagishi, Tomohiro Suzuki","doi":"10.1093/bbb/zbaf084","DOIUrl":"10.1093/bbb/zbaf084","url":null,"abstract":"Ustilago esculenta is a smut fungus that parasitizes Zizania latifolia, forming edible fungal galls known as Makomotake. In this study, we quantified indole-3-acetic acid (IAA) produced in liquid-cultured U. esculenta and in fungal galls at different growth stages. IAA was detected in both samples, and its content showed a tendency to increase during the mature growth stage in October. Furthermore, RNA-seq analysis was performed to investigate gene expression changes in U. esculenta during different developmental stages. The gene involved in spore maturation was upregulated in October, consistent with fungal maturation. Moreover, numerous genes with unknown functions were significantly upregulated, highlighting potential targets for further investigation. These findings provide insights into the molecular mechanisms underlying fungal gall development and the symbiotic relationship between U. esculenta and Z. latifolia, providing a foundation for future research on fungal-plant interactions.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1293-1301"},"PeriodicalIF":1.3,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breeding of the sake yeast K1801 with normal checkpoint integrity by isolating spontaneous cdc55 revertants. 通过分离cdc55自然复归物选育具有正常检查点完整性的清酒酵母K1801。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf089

Tetsuya Goshima, Ikuhisa Nishida, Hitoshi Shimoi, Takeshi Akao, Dai Hirata

引用次数: 0

Brominated bitter compounds from the skin tubercle gland of the stonefish Synanceia verrucosa. 从石鱼的皮肤结节腺中提取的溴化苦味化合物。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf082

Ryoya Kawabe, Sota Hoshi, Toru Hifumi, Akihiko Yamamoto, Hajime Uchida, Masayuki Satake, Hiroshi Nagai

引用次数: 0

Overexpression of GDE1 decreases glycerophosphoinositol in intestinal epithelial cells in vivo. 体内过表达GDE1可降低肠上皮细胞中的甘油磷酸肌醇。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-23 DOI: 10.1093/bbb/zbaf087

Siyi Chen, Rahmawati Aisyah, Ayako Honda, Takahiro Kudo, Yusuke Sotomaru, Satoshi Ishizuka, Thanutchaporn Kumrungsee, Toshihiko Iwanaga, Noriyuki Yanaka

{"title":"Overexpression of GDE1 decreases glycerophosphoinositol in intestinal epithelial cells in vivo.","authors":"Siyi Chen, Rahmawati Aisyah, Ayako Honda, Takahiro Kudo, Yusuke Sotomaru, Satoshi Ishizuka, Thanutchaporn Kumrungsee, Toshihiko Iwanaga, Noriyuki Yanaka","doi":"10.1093/bbb/zbaf087","DOIUrl":"10.1093/bbb/zbaf087","url":null,"abstract":"Glycerophosphoinositol (GPI), a product of phospholipases acting on membrane phosphoinositides, is increasingly being recognized as an important bioactive molecule. However, its cellular functions in vivo remain unexplored. Recently, several mammalian glycerophosphodiesterases (GDEs) have shown to be involved in the catabolism of GPI, possibly regulating its intracellular levels. Given the growing interest in the biological significance of GPI in intestinal epithelial cells, this study focused on screening a GDE involved in intracellular GPI metabolism. Our results showed that GDE1 mRNA was highly expressed in intestinal epithelial cells and that its overexpression decreased intracellular GPI in cultured cells. We then created transgenic mice with GDE1 overexpression specifically in intestinal epithelial cells. These mice showed that GDE1 protein is localized in epithelial cells and that GPI levels are downregulated in the colon epithelial layers. This study provides a novel animal model for exploring GPI functions in vivo.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":"1312-1319"},"PeriodicalIF":1.3,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro functional reconstitution of cofactor-dependent plant cytochrome P450 system on artificial liposomes. 辅助因子依赖性植物细胞色素P450系统在人工脂质体上的体外功能重建。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-21 DOI: 10.1093/bbb/zbaf126

JiaXin Fu, Ayami Ota, Hironori Takeda, Yuzuru Tozawa

引用次数: 0

Synthesis of Farinomaleins and their Antimicrobial Activities. 糠麦芽素的合成及其抗菌活性研究。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-20 DOI: 10.1093/bbb/zbaf125

Md Masud Rana, Taku Oshima, Ei Horiuchi, Issay Osaka, Yasuhiro Igarashi, Daisuke Urabe

引用次数: 0

The aerial part of Panax japonicus alleviates HFD-induced non-alcoholic fatty liver via activation of the AMPK/ACC pathway in rats. 参气部通过激活AMPK/ACC通路减轻大鼠hfd诱导的非酒精性脂肪肝。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-19 DOI: 10.1093/bbb/zbaf123

Miao He, Huachan Zheng, Yundong Xie, Dongdong Zhang, Wenli Huang, Yuze Li, Huawei Zhang, Chong Deng, Jiping Liu, Xiaomei Song, Hong Xu, Yi Jiang

引用次数: 0

Upcycling whey through the production of edible Aspergillus oryzae cell biomass. 通过生产可食用米曲霉细胞生物量对乳清进行升级回收。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-12 DOI: 10.1093/bbb/zbaf122

Masao Nakamura, Ryota Hattori, Satoshi Suzuki, Junichi Mano

引用次数: 0

Mucin-dependent transcriptional dynamics of Akkermansia muciniphila in co-culture with human colonic organoids. 嗜粘阿克曼氏菌与人结肠类器官共培养中粘蛋白依赖的转录动力学。

IF 1.3 4区生物学

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-07 DOI: 10.1093/bbb/zbaf121

Moemi Matsuzaki, Atsuki Imai, Kota Yanagisawa, Tsukasa Oda, Hideo Satsu, Nobuo Sasaki, Eiji Miyauchi

{"title":"Mucin-dependent transcriptional dynamics of Akkermansia muciniphila in co-culture with human colonic organoids.","authors":"Moemi Matsuzaki, Atsuki Imai, Kota Yanagisawa, Tsukasa Oda, Hideo Satsu, Nobuo Sasaki, Eiji Miyauchi","doi":"10.1093/bbb/zbaf121","DOIUrl":"https://doi.org/10.1093/bbb/zbaf121","url":null,"abstract":"Akkermansia muciniphila, a mucin-degrading gut bacterium, contributes to intestinal homeostasis and metabolic disorders, yet its transcriptional response to human colonic mucin remains unclear. Here, we used human colonic organoids derived from either a healthy tissue (wild-type) or ATOH1-knockout lines lacking goblet cell differentiation. Co-culturing wild-type or ATOH1-knockout organoids with A. muciniphila followed by RNA-sequencing revealed distinct transcriptional profiles modulated by mucin availability, particularly affecting genes for growth and stress resistance. Notably, mucin degradation genes exhibited limited responses, contrasting with studies using porcine mucin, highlighting the specificity of human mucin interactions. Conversely, genes for mucin uptake and pili formation (e.g., Amuc_1100), which are crucial for host interaction, were upregulated with wild-type organoids. These results underscore the importance of using physiologically relevant human models. Our findings reveal A. muciniphila's adaptive gene regulation in response to human mucin, offering insights into host-microbe interactions shaped by the mucosal environment.","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0