Improved transformation system of Bacillus subtilis competent cells using PCR DNA fragments as donor DNA.

IF 1.3 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2025-08-05 DOI:10.1093/bbb/zbaf120

Kazuhisa Sawada, Keiji Endo, Masatoshi Tohata, Katsuya Ozaki, Masakazu Kataoka

引用次数: 0

Abstract

To enhance the transformation efficiency of Bacillus subtilis Marburg 168 using PCR DNA fragments, we optimized the competent cell preparation by adding Mn2+ to the competence medium. This Mn2+ addition promoted both cell growth and increased transformation frequency. Moreover, we determined the necessary length of the homologous sequence on each end to achieve practically sufficient transformation through double crossover recombination.

查看原文本刊更多论文

改良的枯草芽孢杆菌能态细胞PCR DNA片段供体转化体系。

为了利用PCR DNA片段提高枯草芽孢杆菌马尔堡168的转化效率，我们在培养培养基中添加Mn2+，优化了适能细胞的制备。Mn2+的加入既促进了细胞生长，又增加了转化频率。此外，我们确定了两端同源序列的必要长度，从而通过双交叉重组实现实际充分的转化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).