Brazilian Journal of Medical and Biological Research最新文献_第5页

Effects of esketamine on depression-like behavior and dendritic spine plasticity in the prefrontal cortex neurons of spared nerve injury-induced depressed mice. 艾司氯胺对神经损伤诱导的抑郁小鼠前额叶皮层神经元抑郁样行为和树突棘可塑性的影响

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-08 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13736

Bixin Huang, Xiaoling Li, Yuling Zheng, Ying Mai, Zhongqi Zhang

{"title":"Effects of esketamine on depression-like behavior and dendritic spine plasticity in the prefrontal cortex neurons of spared nerve injury-induced depressed mice.","authors":"Bixin Huang, Xiaoling Li, Yuling Zheng, Ying Mai, Zhongqi Zhang","doi":"10.1590/1414-431X2024e13736","DOIUrl":"10.1590/1414-431X2024e13736","url":null,"abstract":"The present study utilized the spared nerve injury (SNI) to create a mouse model of depression to investigate the impact of esketamine on depressive-like behaviors, on the expression of PSD-95 and CRMP2 proteins, and on changes in neuronal dendritic spine plasticity in the prefrontal cortex (PFC). Depressive-like behavioral tests were performed 1 h after esketamine treatment, and the PFC tissues were obtained on the fourth day after completing the behavioral tests. Then, dendritic spine density and morphology in the PFC were measured using Golgi staining, and CRMP2 and PSD-95 proteins were obtained from PFC tissue by western blotting. The results of this study showed that esketamine significantly increased the immobility time in the forced swimming test and tail suspension test. In the open field test, esketamine increased the time spent in the open arms, the time spent in the central area, and the total distance covered. It also increased the protein expression levels of CRMP2 and PSD-95 in addition to the total and mature dendritic spine density of the PFC in SNI-depressed mice. Esketamine can significantly improve depression-like behaviors in SNI-depressed mice and promote an increase in dendritic spine density and maturation in the PFC. These effects may be associated with changes in CRMP2 and PSD-95 expression.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FNDC5/Irisin in dementia and cognitive impairment: update and novel perspective. 痴呆症和认知障碍中的 FNDC5/Irisin：最新进展和新观点。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-08 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13447

Xiaofeng Guo, Xiaocheng Huang, Yachao Yang, Luying Dong, Dehuan Kong, Jianmei Zhang

引用次数: 0

ABCG2 polymorphism and rivaroxaban pharmacokinetics in healthy individuals after a single dose. 健康人单次用药后的 ABCG2 多态性和利伐沙班药代动力学。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13257

A F Dos Santos, Q A S Francisco, J B Nunes, F A Colombo, V B Boralli

{"title":"ABCG2 polymorphism and rivaroxaban pharmacokinetics in healthy individuals after a single dose.","authors":"A F Dos Santos, Q A S Francisco, J B Nunes, F A Colombo, V B Boralli","doi":"10.1590/1414-431X2024e13257","DOIUrl":"10.1590/1414-431X2024e13257","url":null,"abstract":"Rivaroxaban is a direct factor Xa inhibitor. Its interindividual variability is large and may be connected to the occurrence of adverse drug reactions or drug inefficacy. Pharmacogenetics studies concentrating on the reasons underlying rivaroxaban's inadequate response could help explain the differences in treatment results and medication safety profiles. Against this background, this study evaluated whether polymorphisms in the gene encoding the ABCG2 transporter modify the pharmacokinetic characteristics of rivaroxaban. A total of 117 healthy volunteers participated in two bioequivalence experiments with a single oral dose of 20 mg rivaroxaban, with one group fasting and the other being fed. Ultra-high-performance liquid chromatography coupled with mass spectrometry was employed to determine the plasma concentrations of rivaroxaban, and the WinNonlin program was used to calculate the pharmacokinetics parameters. In the fasting group, the rivaroxaban pharmacokinetic parameters of Vd (508.27 vs 334.45 vs 275.59 L) and t1/2 (41.04 vs 16.43 vs 15.47 h) were significantly higher in ABCG2 421 A/A genotype carriers than in ABCG2 421 C/C and 421 C/A genotype carriers (P<0.05). The mean values of Cmax (145.81 vs 176.27 vs 190.19 ng/mL), AUC0-t (1193.81 vs 1374.69 vs 1570.77 ng/mL·h), and Cl (11.82 vs 14.50 vs 13.01 mL/h) for these groups were lower, but this difference was not statistically significant (P>0.05). These findings suggested that the ABCG2 421 A/A genotype may impact rivaroxaban parameters after a single dose in healthy subjects. This finding must be validated before it is applied in clinical practice.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ag85B with c-di-AMP as mucosal adjuvant showed immunotherapeutic effects on persistent Mycobacterium tuberculosis infection in mice. 以 c-di-AMP 作为粘膜佐剂的 Ag85B 对小鼠持续性结核分枝杆菌感染有免疫治疗作用。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13409

Xuan Liang, Ruonan Cui, Xue Li, Huanhuan Ning, Jian Kang, Yanzhi Lu, Shan Zhou, Xinying Huang, Yujun Peng, Jingyao Zhang, Shiyun Li, Yanling Ma, Yinlan Bai

{"title":"Ag85B with c-di-AMP as mucosal adjuvant showed immunotherapeutic effects on persistent Mycobacterium tuberculosis infection in mice.","authors":"Xuan Liang, Ruonan Cui, Xue Li, Huanhuan Ning, Jian Kang, Yanzhi Lu, Shan Zhou, Xinying Huang, Yujun Peng, Jingyao Zhang, Shiyun Li, Yanling Ma, Yinlan Bai","doi":"10.1590/1414-431X2024e13409","DOIUrl":"10.1590/1414-431X2024e13409","url":null,"abstract":"Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains the leading cause of mortality by a single infectious agent in the world. M. tuberculosis infection could also result in clinical chronic infection, known as latent TB infection (LTBI). Compared to the current limited treatment, several subunit vaccines showed immunotherapeutic effects and were included in clinical trials. In this study, a subunit vaccine of Ag85B with a novel mucosal adjuvant c-di-AMP (Ag85B:c-di-AMP) was delivered intranasally to a persistent M. tuberculosis H37Ra infection mouse model, which also presented the asymptomatic characteristics of LTBI. Compared with Ag85B immunization, Ag85B:c-di-AMP vaccination induced stronger humoral immune responses, significantly higher CD4+ T cells recruitment, enhanced Th1/Th2/Th17 profile response in the lung, decreased pathological lesions of the lung, and reduced M. tuberculosis load in mice. Taken together, Ag85B:c-di-AMP mucosal route immunization provided an immunotherapeutic effect on persistent M. tuberculosis H37Ra infection, and c-di-AMP, as a promising potential mucosal adjuvant, could be further used in therapeutic or prophylactic vaccine strategies for persistent M. tuberculosis infection as well as LTBI.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between loneliness and mental health among nurses: a cross-sectional research in China. 中国护士孤独感与心理健康的关系：一项横断面研究。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13408

Yu Qiao, Chao Wang, Xueyan Tian, Ming Cao

{"title":"Association between loneliness and mental health among nurses: a cross-sectional research in China.","authors":"Yu Qiao, Chao Wang, Xueyan Tian, Ming Cao","doi":"10.1590/1414-431X2024e13408","DOIUrl":"10.1590/1414-431X2024e13408","url":null,"abstract":"This study explored the association between loneliness and mental health among nurses in China during the COVID-19 pandemic. This cross-sectional study was conducted from March to April 2022. We enrolled 2,811 nurses from a tertiary hospital in China. Demographic characteristics, lifestyle factors, work-related factors, and psychological characteristics were collected from participants via a self-reported questionnaire. Loneliness was measured with the three-item short form of the Revised UCLA Loneliness Scale, and the Patient Health Questionnaire (PHQ-9) and the General Anxiety Disorder (GAD-7) scale were used to measure mental health. Adjusted odds ratios (ORs) and 95% confidence intervals (CI) were determined using binary logistic regression. Among participants in this study, 12.0% (337) experienced loneliness, and 7.8% (219) and 6.7% (189) reported depression and anxiety, respectively. The loneliness scores were categorized into three levels (3, 4-6, and 7-9). For depression, compared with the lowest reference, the ORs and 95% CI across the tertile were 1.31 (0.69-1.84) and 2.53 (1.11-5.76) after adjustment, respectively, and the P-value for trend was 0.045. For anxiety, compared with the lowest reference, the ORs and 95%CI across the tertile were 1.84 (1.28-2.63) and 2.52 (1.57-4.10) after adjustment, respectively; the P-value for trend was 0.004. This study showed that loneliness was significantly associated with poor mental health among nurses during the COVID-19 pandemic. These findings suggested that medical establishments should offer interventions for nurses to prevent mental health problems by targeting this modifiable risk factor.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemoprotective effect of arbutin on azoxymethane-induced aberrant crypt foci in rat colon via modulation of PCNA/Bax protein. 熊果苷通过调节 PCNA/Bax 蛋白对偶氮甲烷诱导的大鼠结肠异常隐窝病灶的化学保护作用

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13306

K A Ahmed, S H Shareef, T A Faraj, M A Abdulla, S K Najmaldin, N F S Agha, R K Kheder

{"title":"Chemoprotective effect of arbutin on azoxymethane-induced aberrant crypt foci in rat colon via modulation of PCNA/Bax protein.","authors":"K A Ahmed, S H Shareef, T A Faraj, M A Abdulla, S K Najmaldin, N F S Agha, R K Kheder","doi":"10.1590/1414-431X2024e13306","DOIUrl":"10.1590/1414-431X2024e13306","url":null,"abstract":"Arbutin is utilized in traditional remedies to cure numerous syndromes because of its anti-microbial, antioxidant, and anti-inflammatory properties. This study aimed to evaluate chemopreventive effects of arbutin on azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) in rats. Five groups of rats were used: normal control group (rats injected hypodermically with sterile phosphate-buffered saline once per week for two weeks) and groups 2-5, which were subcutaneously inoculated with 15 mg/kg AOM once a week for two weeks. AOM control and 5-fluorouracil (5-FU) control groups were fed 10% Tween orally daily for 8 weeks using a feeding tube. The treated groups were fed 30 and 60 mg/kg arbutin every day for 2 months. ACF from the AOM control group had aberrant nuclei in addition to multilayered cells and an absence of goblet cells. The negative control group displayed spherical cells and nuclei in basal positions. Histological examination revealed a reduced number of AFC cells from colon tissues of the 5-FU reference group. Arbutin-fed animals showed down-regulation of proliferating cell nuclear antigen (PCNA) and up-regulation of Bax protein compared to AOM control. Rats fed with arbutin displayed a significant increase of superoxide dismutase (SOD) and catalase (CAT) activities in colon tissue homogenates compared to the AOM control group. In conclusion, arbutin showed therapeutic effects against colorectal cancer, explained by its ability to significantly decrease ACF, down-regulate PCNA protein, and up-regulate Bax protein. In addition, arbutin significantly increased SOD and CAT, and decreased malondialdehyde (MDA) levels, which might be due to its anti-proliferative and antioxidant properties.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nandrolone decanoate impairs gastrointestinal motility and duodenal morphometry in moderately exercised rats. 癸酸诺龙会损害中度运动大鼠的胃肠道蠕动和十二指肠形态。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13452

A T Hauschildt, L A Gama, G T Volpato, L A Corá, A A V Silva, M O Belém, P J C Magalhães, A A Santos, M F Américo

{"title":"Nandrolone decanoate impairs gastrointestinal motility and duodenal morphometry in moderately exercised rats.","authors":"A T Hauschildt, L A Gama, G T Volpato, L A Corá, A A V Silva, M O Belém, P J C Magalhães, A A Santos, M F Américo","doi":"10.1590/1414-431X2024e13452","DOIUrl":"10.1590/1414-431X2024e13452","url":null,"abstract":"The misuse of anabolic androgenic steroid associated or not with physical workouts disrupts gastrointestinal (GI) function homeostasis. Our goal was to investigate the effects of nandrolone decanoate (ND) and moderate swimming on the GI transit of solid meals, GI motor contractility, and intestinal histology in rats. Male Wistar rats were allocated to four groups that received intramuscular injections of ND (5.0 mg/kg) or vehicle (60.0 µL) and were submitted or not to swimming sessions (60 min, 5% body weight overload) for 4 weeks. Gastric emptying, intestinal transit, in vitro GI contractility, intestinal morphometry, and duodenal mucosal mast cells were evaluated in all experimental groups. ND treatment accelerated gastric emptying, slowed small intestine transit time, enhanced gastric carbachol-mediated reactivity, decreased crypt depth and villus height, reduced mucosal thickness, and increased the circular and longitudinal muscle layer thickness of the duodenum in sedentary rats. Moderate exercise accelerated intestinal transit time and reduced submucosa thickness. In vehicle-treated animals, a strong negative correlation was found between intestinal transit and mucosal mast cells, which was reversed by ND treatment. Combining ND treatment and swimming accelerated gastric emptying, increased duodenal cholinergic reactivity, inhibited the sodium nitroprusside relaxing response, increased the number of duodenal mast cells, decreased villus height, and increased the thickness of all muscle layers. ND changed the morphological and functional properties of the GI tract over time, with intense dysmotility, especially in sedentary animals, but moderate exercise seemed to have played a compensatory role in these harmful effects in the gut.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining metabolomics and network pharmacology to investigate the protective effect of Jiawei Xinglou Chengqi Granules in ischemic stroke. 结合代谢组学和网络药理学研究加味杏楼承气颗粒对缺血性中风的保护作用

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13388

Raoqiong Wang, Linshen Mao, Pan Liang, Yulu Gan, Qixue Gao, Shizhi Liang, Dechou Zhang, Gang Luo, Sijin Yang

{"title":"Combining metabolomics and network pharmacology to investigate the protective effect of Jiawei Xinglou Chengqi Granules in ischemic stroke.","authors":"Raoqiong Wang, Linshen Mao, Pan Liang, Yulu Gan, Qixue Gao, Shizhi Liang, Dechou Zhang, Gang Luo, Sijin Yang","doi":"10.1590/1414-431X2024e13388","DOIUrl":"10.1590/1414-431X2024e13388","url":null,"abstract":"Jiawei Xinglou Chengqi Granule (JXCG) is an effective herbal medicine for the treatment of ischemic stroke (IS). JXCG has been shown to effectively ameliorate cerebral ischemic symptoms in clinical practice, but the underlying mechanisms are unclear. In this study, we investigated the mechanisms of action of JXCG in the treatment of IS by combining metabolomics with network pharmacology. The chemical composition of JXCG was analyzed using ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Ultra-high performance liquid chromatography-tandem time-of-flight mass spectrometry (UHPLC-Q-TOF MS) untargeted metabolomics were used to identify differential metabolites within metabolic pathways. Network pharmacology was applied to mine potential targets of JXCG in the treatment of IS. The identified key targets were validated by constructing an integrated network of metabolomics and network pharmacology and by molecular docking using Cytoscape. The effect of JXCG on IS was evaluated in vivo, and the predicted targets and pathways of JXCG in IS therapy were assessed using immunoblotting. Combining metabolomics and network pharmacology, we identified the therapeutic targets of JXCG for IS. Notably, JXCG lessened neuronal damage and reduced cerebral infarct size in rats with IS. Western blot analysis showed that JXCG upregulated PRKCH and downregulated PRKCE and PRKCQ proteins. Our combined network pharmacology and metabolomics findings showed that JXCG may have therapeutic potential in the treatment of IS by targeting multiple factors and pathways.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FRAX486, a PAK inhibitor, overcomes ABCB1-mediated multidrug resistance in breast cancer cells. PAK抑制剂FRAX486能克服乳腺癌细胞中ABCB1介导的多药耐药性。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13357

Meng Zhang, Xiaoqi Zeng, Meiling She, Xingduo Dong, Jun Chen, Qingquan Xiong, Guobin Qiu, Shuyi Yang, Xiangqi Li, Guanghui Ren

{"title":"FRAX486, a PAK inhibitor, overcomes ABCB1-mediated multidrug resistance in breast cancer cells.","authors":"Meng Zhang, Xiaoqi Zeng, Meiling She, Xingduo Dong, Jun Chen, Qingquan Xiong, Guobin Qiu, Shuyi Yang, Xiangqi Li, Guanghui Ren","doi":"10.1590/1414-431X2024e13357","DOIUrl":"10.1590/1414-431X2024e13357","url":null,"abstract":"The overexpression of P-glycoprotein (P-gp/ABCB1) is a leading cause of multidrug resistance (MDR). Hence, it is crucial to discover effective pharmaceuticals that counteract ABCB1-mediated multidrug resistance. FRAX486 is a p21-activated kinase (PAK) inhibitor. The objective of this study was to investigate whether FRAX486 can reverse ABCB1-mediated multidrug resistance, while also exploring its mechanism of action. The CCK8 assay demonstrated that FRAX486 significantly reversed ABCB1-mediated multidrug resistance. Furthermore, western blotting and immunofluorescence experiments revealed that FRAX486 had no impact on expression level and intracellular localization of ABCB1. Notably, FRAX486 was found to enhance intracellular drug accumulation and reduce efflux, resulting in the reversal of multidrug resistance. Docking analysis also indicated a strong affinity between FRAX486 and ABCB1. This study highlights the ability of FRAX486 to reverse ABCB1-mediated multidrug resistance and provides valuable insights for its clinical application.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11221864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ACE gene polymorphisms (rs4340) II and DI are more responsive to the ergogenic effect of caffeine than DD on aerobic power, heart rate, and perceived exertion in a homogeneous Brazilian group of adolescent athletes. 在巴西的一个同质青少年运动员群体中，ACE基因多态性（rs4340）II和DI比DD对咖啡因对有氧功率、心率和感觉用力的促进作用反应更灵敏。

IF 1.9 4区医学

Brazilian Journal of Medical and Biological Research Pub Date : 2024-06-17 eCollection Date: 2024-01-01 DOI: 10.1590/1414-431X2024e13217

H Spineli, M Dos Santos, D Almeida, D Gitaí, M Silva-Cavalcante, P Balikian, T Ataide-Silva, A Marinho, F Sousa, G de Araujo

{"title":"ACE gene polymorphisms (rs4340) II and DI are more responsive to the ergogenic effect of caffeine than DD on aerobic power, heart rate, and perceived exertion in a homogeneous Brazilian group of adolescent athletes.","authors":"H Spineli, M Dos Santos, D Almeida, D Gitaí, M Silva-Cavalcante, P Balikian, T Ataide-Silva, A Marinho, F Sousa, G de Araujo","doi":"10.1590/1414-431X2024e13217","DOIUrl":"10.1590/1414-431X2024e13217","url":null,"abstract":"The purpose of this study was to verify the association between angiotensin-converting enzyme (ACE) genotypes DD, DI, and II and caffeine (CAF) ingestion on endurance performance, heart rate, ratio of perceived exertion (RPE), and habitual caffeine intake (HCI) of adolescent athletes. Seventy-four male adolescent athletes (age: DD=16±1.7; DI=16±2.0; II=15±1.7 years) ingested CAF (6 mg/kg) or placebo (PLA) one hour before performing the Yo-Yo Intermittent Recovery level 1 (Yo-Yo IR1) test. No difference was found among groups for HCI. However, CAF increased the maximal distance covered and VO2max in DI and II genotype carriers compared to PLA (DD: Δ=31 m and 0.3 mL·kg-1·min-1; DI: Δ=286 m and 1.1 mL·kg-1·min-1; II: Δ=160 m and 1.4 mL·kg-1·min-1). Heart rate of DI and II genotype carriers increased with CAF compared to PLA, while RPE was higher in the II and lower in the DD genotypes. The correlations between HCI and maximal distance covered or VO2max were significant in the II genotype carriers with CAF. CAF increased endurance capacity, heart rate, and RPE in adolescent athletes with allele I, while endurance performance and aerobic power had a positive correlation to HCI in the II genotype group. These findings suggested that DD genotype were less responsive to CAF and that genetic variations should be taken into account when using CAF supplementation to enhance exercise performance.","PeriodicalId":9088,"journal":{"name":"Brazilian Journal of Medical and Biological Research","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0