Biotechnic & Histochemistry最新文献_第6页

Pravastatin attenuates isoprenaline induced cardiac fibrosis in a mouse model. 普伐他汀在小鼠模型中减轻异丙肾上腺素诱导的心脏纤维化。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10520295.2023.2260303

Abhinav Rana, Thakur Uttam Singh, Meemansha Sharma, Manju Gari, Tarun Kumar, Subhashree Parida, Madhu Cholenahalli Lingaraju, Asok Kumar Mariappan, Akhilesh Kumar, Dinesh Kumar

{"title":"Pravastatin attenuates isoprenaline induced cardiac fibrosis in a mouse model.","authors":"Abhinav Rana, Thakur Uttam Singh, Meemansha Sharma, Manju Gari, Tarun Kumar, Subhashree Parida, Madhu Cholenahalli Lingaraju, Asok Kumar Mariappan, Akhilesh Kumar, Dinesh Kumar","doi":"10.1080/10520295.2023.2260303","DOIUrl":"10.1080/10520295.2023.2260303","url":null,"abstract":"We investigated the effects of pravastatin (PRAVA) on isoprenaline (ISP) induced cardiac fibrosis using four groups of mice: untreated control, PRAVA, ISP, ISP + PRAVA groups. ISP, 20 mg/kg, was administered subcutaneously daily for 14 days. PRAVA, 20 mg/kg, was administered orally daily for 14 days. Mice were sacrificed on day15 and heart and blood samples were collected to investigate cardiac injury markers. The mean body weight for the ISP group on day 15 was decreased significantly compared to day 0; PRAVA increased the mean body weight slightly on day 15 of treatment compared to day 0. The heart:body weight ratio was increased in the ISP group compared to the control group, but the ratio was returned to near control ratio in the PRAVA + ISP group. The serum creatine kinase-myocardial band (CK-MB) level was reduced significantly in the PRAVA + ISP group compared to the ISP group. Serum triglyceride level was decreased significantly in ISP + PRAVA group compared to the ISP group. PRAVA administration significantly reduced tissue collagen I and III levels in the ISP + PRAVA group compared to the ISP group. Lipid oxidation was decreased and reduced glutathione activity was increased in the PRAVA + ISP group compared to the ISP group. IL-6, α-SMA, CTGF, TGF-β and SMAD-3 gene expressions were decreased in the PRAVA + ISP group compared to the ISP group. We found fewer inflammatory cells and less fibrosis in heart tissue in the PRAVA + ISP group compared to the ISP group. PRAVA decreased ISP induced cardiac fibrosis by reducing oxidative stress, collagen deposition and inflammation, as well as by decreasing expression of TGF-β, SMAD-3 and CTGF genes.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cocaine and amphetamine regulated transcript (CART): a newly characterized neuropeptide in human prostate. 可卡因和苯丙胺调节转录物（CART）：人类前列腺中一种新表征的神经肽。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-08-24 DOI: 10.1080/10520295.2023.2245328

Cyprian Weaver, Marie Antony, Jack Fite, Paari Murugan, Andrew C Nelson, Juan C Manivel

{"title":"Cocaine and amphetamine regulated transcript (CART): a newly characterized neuropeptide in human prostate.","authors":"Cyprian Weaver, Marie Antony, Jack Fite, Paari Murugan, Andrew C Nelson, Juan C Manivel","doi":"10.1080/10520295.2023.2245328","DOIUrl":"10.1080/10520295.2023.2245328","url":null,"abstract":"Cocaine and amphetamine regulated transcript (CART) is a somatostatin-like polypeptide. CART has been localized in the CNS, hypothalamo-pituitary-adrenocortical (HPA) axis, pancreatic islets and enteric nervous system. We investigated the cellular localization of CART in normal human prostate, benign prostatic hyperplasia, prostatic intraepithelial neoplasia and acinar adenocarcinoma. CART was assessed using immunohistochemistry (IHC) and in situ hybridization (ISH), and its gene expression was identified by RTqPCR. We found cellular expression of CART in both normal prostatic luminal secretory epithelial cells neuroendocrine cells (NEC) of both ducts and acini. The cellular appearance indicated a cycle of neuropeptide synthesis and secretion as validated by ISH/IHC concordance. RTqPCR analysis also validated the immunohistochemical data and gene expression, which both indicated low to moderate expression in prostatic tissues. CART expression also was increased in both neuroendocrine and glandular epithelial cell populations from samples of benign prostatic hyperplasia as validated by IHC, ISH and RTqPCR. CART expression was markedly diminished and, in some cases, entirely absent in tissues of prostatic intraepithelial neoplasia and adenocarcinoma. Owing to loss of CART expression in adenocarcinoma and its increase in benign prostatic hyperplasia, CART may prove to be an important prostate marker.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10415540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disulfiram ameliorates bleomycin induced pulmonary inflammation and fibrosis in rats. 二硫仑改善博莱霉素诱导的大鼠肺部炎症和纤维化。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10520295.2023.2261367

Negar Hamidi, Farideh Feizi, Abbas Azadmehr, Ebrahim Zabihi, Soraya Khafri, Zeinab Zarei-Behjani, Zahra Babazadeh

{"title":"Disulfiram ameliorates bleomycin induced pulmonary inflammation and fibrosis in rats.","authors":"Negar Hamidi, Farideh Feizi, Abbas Azadmehr, Ebrahim Zabihi, Soraya Khafri, Zeinab Zarei-Behjani, Zahra Babazadeh","doi":"10.1080/10520295.2023.2261367","DOIUrl":"10.1080/10520295.2023.2261367","url":null,"abstract":"Bleomycin (BL) is a widely used anticancer drug that can cause pulmonary fibrosis due to increased fibroblast proliferation and increased secretion of extracellular matrix. RASSF1A is a tumor suppressor gene that is down-regulated by DNA methylation during fibrosis. Disulfiram (DSF), a noncytosine DNA methyltransferase inhibitor, can revert epigenetic biomarkers and re-express silenced genes. We investigated anti-inflammatory and anti-fibrotic effects of DSF on regulation of epigenetic molecules and histopathology in a rat model of BL induced pulmonary fibrosis. We used six groups of rats: sesame oil (SO) control (Co) group, BL group, BL + SO group and three BL + DSF groups administered 1 mg/kg DSF (BL + DSF), 10 mg/kg DSF (BL + DSF10) or 100 mg/kg DSF (BL + DSF100), respectively. BL was administered intratracheally to induce pulmonary fibrosis. DSF and SO were injected intraperitoneally (i.p.) 2 days before BL administration; these injections were continued for 3 weeks. At the end of the study, lung tissues were removed for molecular and histopathologic studies. Administration of 10 or 100 mg/kg DSF after BL induced pulmonary inflammation and fibrosis, and up-regulated RASSF1A and down-regulated TNF-α and IL-1 β compared to the BL and BL + SO groups. A RASSF1A unmethylated band was observed using the methylation-specific PCR technique in rats that had been administered 10 and 100 mg/kg DSF, which indicated partial DNA demethylation. Histopathologic evaluation revealed that fibrosis and all inflammatory scores were decreased significantly in the BL + DSF10 and BL + DSF100 groups compared to the BL group. Our findings indicate that DSF modified DNA methylation by up-regulating RASSF1A, which reduced inflammation and fibrosis in BL induced pulmonary inflammation and fibrosis.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41115583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stains recently certified. 污渍最近得到认证。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10520295.2023.2262844

引用次数: 0

Effects of nicorandil on QT prolongation and myocardial damage caused by citalopram in rats. 尼可地尔对西酞普兰所致大鼠QT延长及心肌损伤的影响。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-07-19 DOI: 10.1080/10520295.2023.2233417

Gozde Akturk, Serap Cilaker Micili, Ozlem Gursoy Doruk, Nil Hocaoglu, Pinar Akan, Bekir Ugur Ergur, Samar Ahmed, Sule Kalkan

{"title":"Effects of nicorandil on QT prolongation and myocardial damage caused by citalopram in rats.","authors":"Gozde Akturk, Serap Cilaker Micili, Ozlem Gursoy Doruk, Nil Hocaoglu, Pinar Akan, Bekir Ugur Ergur, Samar Ahmed, Sule Kalkan","doi":"10.1080/10520295.2023.2233417","DOIUrl":"10.1080/10520295.2023.2233417","url":null,"abstract":"Citalopram is a selective serotonin re-uptake inhibitor (SSRI) antidepressant; it exhibits the greatest cardiotoxic effect among SSRIs. Citalopram can cause drug-induced long QT syndrome (LQTS) and ventricular arrhythmias. We investigated the protective effect of nicorandil, a selective mitochondrial KATP (mito-KATP) channel opener, on LQTS and myocardial damage caused by citalopram in male rats. In a preliminary study, we determined that the minimum citalopram dose that prolonged the QT interval was 102 mg/kg injected intraperitoneally. For the main study, rats were divided randomly into five experimental groups: untreated control, normal saline + citalopram, nicorandil + citalopram, 5-hydroxydecanoate (5-HD) + citalopram, 5-HD + nicorandil + citalopram. Biochemical and histologic data from blood and heart tissue samples from six untreated control rats were evaluated. Electrocardiographic parameters including QRS duration, QT interval, corrected QT interval (QTc) and heart rate (HR) were assessed, and biochemical parameters including malondialdehyde, reduced glutathione, glutathione peroxidase, superoxide dismutase were measured. We also performed histomorphologic and immunohistochemical examination of heart tissue. Citalopram prolonged QT-QTc intervals significantly and increased significantly the histomorphologic score and proportion of apoptotic cells, but produced no differences in the oxidant and antioxidant parameters. Nicorandil did not prevent citalopram induced QT-QTc interval prolongation and produced no significant changes in oxidant and antioxidant parameters; however, it did reduce histologic damage and apoptosis caused by citalopram.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9886832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of Arum maculatum against dextran sulfate sodium induced colitis in rats. 黄对右旋糖酐硫酸钠诱导的大鼠结肠炎的保护作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-07-03 DOI: 10.1080/10520295.2023.2225226

Gülsüm Toparlı Doğan, Remziye Aysun Kepekçi, Nuray Bostancıeri, Mehmet Tarakçıoğlu

{"title":"Protective effect of Arum maculatum against dextran sulfate sodium induced colitis in rats.","authors":"Gülsüm Toparlı Doğan, Remziye Aysun Kepekçi, Nuray Bostancıeri, Mehmet Tarakçıoğlu","doi":"10.1080/10520295.2023.2225226","DOIUrl":"10.1080/10520295.2023.2225226","url":null,"abstract":"Ulcerative colitis (UC) is an inflammatory disease of the large intestine that is characterized by diarrhea, bloody stools, abdominal pain and mucosal ulceration. UC is treated with nonsteroidal anti-inflammatory drugs, corticosteroids or immunosuppressants, but long-term use of these drugs can cause adverse effects. Arum maculatum is used as a traditional treatment for digestive system disorders, but its use for treatment of UC has not been investigated rigorously. We investigated the possible protective effect of a methanol extract of A. maculatum against dextran sulfate sodium (DSS) induced experimental UC in rats. Total phenolic and flavonoid contents of the extract were 32.919 ± 1.125 mg gallic acid equivalent (GAE)/g and 52.045 ± 7.902 µg rutin equivalent (RE)/mg, respectively. The half-maximal inhibitory concentration (IC50) for the extract was 105.76 µg/ml according to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay. Effects of A. maculatum extract on UC induced by DSS were assessed both macroscopically and histologically. We also investigated effects of A. maculatum extract on malondialdehyde (MDA) levels and the oxidative stress index (OSI) in normal rats and rats with UC. We found that treatment with A. maculatum extract protected the colon against DSS induced UC in a dose-dependent manner.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9740465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using extract from alkanet (Alkanna tinctoria) as a source of both a red lipid stain and a blue counterstain for histology. 使用烷烃提取物（Alkanna tinctoria）作为组织学红色脂质染色和蓝色复染的来源。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10520295.2023.2271397

Hayfaa A Alshamar, Richard W Dapson

引用次数: 0

Immunohistochemical evaluation of hormones secreted by pancreatic endocrine tumors. 胰腺内分泌肿瘤分泌激素的免疫组织化学评价。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10520295.2023.2260307

Anne Mones, Sheila Criswell

引用次数: 0

Tribute to Chuck Willis. 致敬查克·威利斯。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2205205

Richard Dapson

{"title":"Tribute to Chuck Willis.","authors":"Richard Dapson","doi":"10.1080/10520295.2023.2205205","DOIUrl":"https://doi.org/10.1080/10520295.2023.2205205","url":null,"abstract":"I met Chuck Willis in the 1980’s at one of the annual meetings of the Biological Stain Commission. I had known about the Commission’s activities with dyes for years and had always sought out Commissioncertified products for my teaching and research activities. What I did not realize, however, was the amount of work involved behind those little certification labels. At our formal dinner that year, I had the good fortune to sit close to Chuck and ply him with questions. He described some of the tests, and as my curiosity grew, he described the record-keeping aspects. He gave many details, but characteristically omitted something very important: he was the one who did so much of the work. Decades later, as I was reviewing certification procedures for possible revision, I obtained copies of pertinent paperwork. There in Chuck’s handwriting were not only the “facts,” but annotations that revealed just how perceptive he was. It became obvious to me that his dedication to detail truly enhanced the value of the BSC Lab’s archives. Each time we met in Rochester, NY, we made time to simply sit and get to know one another better. Frequently his wife, Lorraine, and my wife, Janet, joined in. We learned about Chuck’s dedication to youth and how he coached them for many years in the art and skill of camping. He obviously derived a great personal satisfaction in helping those boys. His stories shed a bright light upon this man whom I had known previously only as a meticulous record keeper and lab worker. On behalf of the Biological Stain Commission, I thank you, Chuck Willis, for being so instrumental in building our organization.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9447222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Charles T. Willis April 17, 1929-December 17, 2022. 查尔斯·威利斯1929年4月17日- 2022年12月17日

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI: 10.1080/10520295.2023.2196894

Chad Fagan

引用次数: 0