Biotechnic & Histochemistry最新文献_第6页

Investigation of the heat shock protein 70 activity in intestine cells of goats with coccidiosis. 山羊球虫病肠细胞热休克蛋白70活性的研究。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-12-04 DOI: 10.1080/10520295.2024.2427790

Mehmet Hesapçıoğlu, Hamdi Avcı

引用次数: 0

Grape seed oil attenuates sodium arsenite-induced gastric, hepatic and colonic damage in Wistar rats. 葡萄籽油减轻亚砷酸钠诱发的 Wistar 大鼠胃、肝和结肠损伤。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1080/10520295.2024.2426049

Akinleye Stephen Akinrinde, Stephen Oluwasemiloore Oyewole, Abimbola Olumide Adekanmbi

{"title":"Grape seed oil attenuates sodium arsenite-induced gastric, hepatic and colonic damage in Wistar rats.","authors":"Akinleye Stephen Akinrinde, Stephen Oluwasemiloore Oyewole, Abimbola Olumide Adekanmbi","doi":"10.1080/10520295.2024.2426049","DOIUrl":"10.1080/10520295.2024.2426049","url":null,"abstract":"Arsenic exposure is associated with numerous morbidities due to dysfunction of various organ systems including the gastrointestinal tract. We investigated the protective effect of grape seed oil (GSO) against sodium arsenite (NaAsO2)-induced gastric, hepatic and colonic injuries in rats. Twenty-four male Wistar rats were divided into four groups of six as follows: Group A (control) received saline; group B received NaAsO2 (2.5 mg/kg) orally for 7 days; group C were treated concurrently with NaAsO2 and GSO (2 ml/kg), while group D received only GSO. Administration of NaAsO2 induced significant (p < 0.05) increases in alanine aminotransferase (ALT) and aspartate aminotransferase (AST); increased periodic acid Schiff (PAS) staining for mucus and increased goblet cell numbers in the stomach and colon; inflammatory cell infiltration and vascular congestion and alterations in the fecal bacterial flora. GSO supplementation generally promoted a reversal of changes induced by NaAsO2 towards control levels. Additionally, there was increased immunohistochemically detected expression of colonic B-cell lymphoma-1 (Bcl-2) and cytokeratins AE1/AE3, but reduced expression of mucin 1 (MUC1) and carcinoembryonic antigen (CEA) in NaAsO2 + GSO and GSO treated rats when compared with the NaAsO2 group. These results suggest that GSO promoted anti-inflammatory processes in the liver, stomach and colon, as well as opposing apoptosis in the colon, resulting in significant attenuation of damage to these tissues.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"414-425"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histochemical and morphological evaluation of a glyoxal acid-free fixative. 无乙二醛酸固定液的组织化学和形态学评估

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2023.2300797

Chelsea Peeler, Christopher R Pitzer, Hector G Paez, Sheila Criswell

{"title":"Histochemical and morphological evaluation of a glyoxal acid-free fixative.","authors":"Chelsea Peeler, Christopher R Pitzer, Hector G Paez, Sheila Criswell","doi":"10.1080/10520295.2023.2300797","DOIUrl":"10.1080/10520295.2023.2300797","url":null,"abstract":"The application of most chemical fixatives, such as formalin, in the anatomic pathology laboratory requires safety training and hazardous chemical monitoring due to the toxicity and health risks associated with their use. Consequently, the use of formalin has been banned in most applications in Europe; the primary exception is its use in the histology laboratory in lieu of a suitable and safer alternative. Glyoxal based solutions, several of which are available commercially, are the most promising alternative fixatives, because they are based on a mechanism of fixation similar to that of formalin. Unlike formalin, however, glyoxal based solutions do not dissociate from water and therefore do not require ventilation measures such as a fume hood. A primary barrier to the adoption of commercially available glyoxal based solutions is their low pH, which can produce undesirable morphological and antigenic tissue alterations; however, a recently available neutral pH glyoxal product (glyoxal acid free) (GAF) has been developed to mitigate the challenges of low pH. We compared the morphology and histochemistry among tissues fixed in 10% neutral buffered formalin, a commercially available acidic glyoxal product (Prefer), and GAF. Tissues fixed in formalin and Prefer exhibited similar morphology and staining properties; tissues fixed with 2% GAF exhibited deleterious effects.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"49-58"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The number and distribution of proliferating cells in the rat's rostral migratory stream as identified by means of two different proliferation markers. 用两种不同的增殖标记物鉴定大鼠喙迁徙流中增殖细胞的数量和分布。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 DOI: 10.1080/10520295.2024.2424252

Kamila Fabianová, Adam Raček, Alexandra Popovičová, Marcela Martončíková, Enikő Račeková

{"title":"The number and distribution of proliferating cells in the rat's rostral migratory stream as identified by means of two different proliferation markers.","authors":"Kamila Fabianová, Adam Raček, Alexandra Popovičová, Marcela Martončíková, Enikő Račeková","doi":"10.1080/10520295.2024.2424252","DOIUrl":"10.1080/10520295.2024.2424252","url":null,"abstract":"In the brains of adult rodents, the cells arising in the subventricular zone of the lateral ventricles maintain the ability to divide when migrating to the olfactory bulb along the rostral migratory stream (RMS). Dividing cells in the RMS are most frequently revealed through immunohistochemical detection of an exogenous marker of proliferation, 5-Bromo-2-deoxyuridine (BrdU), which incorporates into DNA during the S-phase of mitosis. The more recently recognized antigen Ki-67 (also known as Kiel-67 and MKI67), an endogenous protein expressed in nuclei at all stages of mitosis, is also used for proliferation detection. BrdU and Ki-67 are often used as alternative methods, but they have not previously been compared in the RMS. We analyzed the numbers and distribution of cells labeled either with BrdU or Ki-67 within the RMS of adult rats. The first group of animals received a single i.p. dose of BrdU. In the second group, dividing cells were visualized by Ki-67 immunohistochemistry. Some sections from brains of BrdU-treated rats were also immunostained for Ki-67. Labeled cells were counted in the three anatomical parts of the RMS (vertical arm, elbow and horizontal arm) using a method for unbiased estimation of cell density. The distribution of proliferating cells was similar for both markers. Most BrdU and Ki-67 positive cells were located in the vertical arm and in the elbow, but a caudo-rostral reduction in cell divisions was more evident with Ki-67 labeling. The number of Ki-67 positive cells significantly exceeded the number of BrdU positive cells in all parts of the RMS. Our results indicate that BrdU and Ki-67 are not interchangeable markers for evaluation of proliferative activity in the RMS.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"405-413"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Romanowsky staining: history, recent advances and future prospects from a chemistry perspective. 罗曼诺夫斯基染色：从化学角度看历史、最新进展和未来前景。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2023.2273860

Valeriy Kalinin, Pavel Padnya, Ivan Stoikov

{"title":"Romanowsky staining: history, recent advances and future prospects from a chemistry perspective.","authors":"Valeriy Kalinin, Pavel Padnya, Ivan Stoikov","doi":"10.1080/10520295.2023.2273860","DOIUrl":"10.1080/10520295.2023.2273860","url":null,"abstract":"Romanowsky staining was an important methodological breakthrough in diagnostic hematology and cytopathology during the late 19th and early 20th centuries; it has facilitated for decades the work of biologists, hematologists and pathologists working with blood cells. Despite more than a century of studying Romanowsky staining, no systematic review has been published that explains the chemical processes that produce the \"Romanowsky effect\" or \"Romanowsky-Giemsa effect\" (RGE), i.e., a purple coloration arising from the interaction of an azure dye with eosin and not due merely to their simultaneous presence. Our review is an attempt to build a bridge between chemists and biomedical scientists and to summarize the available data on methylene blue (MB) demethylation as well as the related reduction and decomposition of MB to simpler compounds by both light and enzyme systems and microorganisms. To do this, we analyze modern data on the mechanisms of MB demethylation both in the presence of acids and bases and by disproportionation due to the action of light. We also offer an explanation for why the RGE occurs only when azure B, or to a lesser extent, azure A is present by applying experimental and calculated physicochemical parameters including dye-DNA binding constants and electron density distributions in the molecules of these ligands. Finally, we discuss modern techniques for obtaining new varieties of Romanowsky dyes by modifying previously known ones. We hope that our critical literature study will help scientists understand better the chemical and physicochemical processes and mechanisms of cell staining with such dyes.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-20"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the protective effects of silymarin and thymoquinone in the focal cerebral ischemia-reperfusion rat model. 比较水飞蓟素和胸腺醌在局灶性脑缺血再灌注大鼠模型中的保护作用

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-11-07 DOI: 10.1080/10520295.2024.2421511

Merve Solmaz, Ender Erdogan, Dervis Dasdelen, Rasim Mogulkoc, Husamettin Vatansev, Fikret Akyurek, Hanefi Ozbek

{"title":"Comparison of the protective effects of silymarin and thymoquinone in the focal cerebral ischemia-reperfusion rat model.","authors":"Merve Solmaz, Ender Erdogan, Dervis Dasdelen, Rasim Mogulkoc, Husamettin Vatansev, Fikret Akyurek, Hanefi Ozbek","doi":"10.1080/10520295.2024.2421511","DOIUrl":"10.1080/10520295.2024.2421511","url":null,"abstract":"Silymarin and thymoquinone exert neuroprotective effects, although their combined effects in focal cerebral ischemia/reperfusion (I/R) models are unknown. We compared the effect of silymarin and thymoquinone in an I/R rat model. Wistar rats were divided into five groups: SHAM, REP (I/R), SIR (200 mg/kg silymarin+I/R), TIR (3 mg/kg thymoquinone+I/R), and STIR (200 mg/kg silymarin+3-mg thymoquinone+I/R). The rats underwent bilateral carotid artery occlusion for 30 min and neurological assessments 24 h thereafter. Apoptosis was evaluated using anti-caspase-3 and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assays. Astrocyte activation was determined using an anti-GFAP antibody. Total antioxidant status (TAS), total oxidant status (TOS), and trimethylamine N-oxide (TMAO) levels were measured. SHAM and REP rats had the lowest and highest neurological scores, respectively (p = 0.001). REP rats showed greater deterioration than SIR, TIR, and STIR rats. SIR, TIR, and STIR rats had fewer TUNEL and caspase-3-positive cells than REP rats (p<0.05). GFAP expression was higher in REP rats (p<0.05) than in SIR, TIR, and STIR rats (p<0.05). SIR and TIR rats showed higher TAS than REP rats (p<0.05). SIR, TIR, and STIR rats had lower TMAO values than REP and SHAM rats (p<0.05). Silymarin/thymoquinone reduces impairment, apoptosis, and astrocyte activation. Combination therapy reduces TMAO levels.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"387-404"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The renaissance of multipurpose AMeX method over conventional tissue processing method in the current diagnostic era: a comparative study. 在当前诊断时代，多用途AMeX方法相对于传统组织处理方法的复兴：一项比较研究。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-12-04 DOI: 10.1080/10520295.2024.2434743

Hanspal Singh, Priya Kumar, Aadithya B Urs, Jeyaseelan Augustine, Sujata Mohanty, Somesh Kumar, Bhaskar Narayan

引用次数: 0

Stains recently certified. 污渍最近通过了认证。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2024.2286747

引用次数: 0

Protective effects of chlorogenic acid against cyclophosphamide induced liver injury in mice. 绿原酸对环磷酰胺所致小鼠肝损伤的保护作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2023.2287452

Hao Hao, Youmei Xu, Rui Chen, Shanshan Qi, Xiang Liu, Beibei Lin, Xiaohua Chen, Xiaoying Zhang, Lijuan Yue, Chen Chen

{"title":"Protective effects of chlorogenic acid against cyclophosphamide induced liver injury in mice.","authors":"Hao Hao, Youmei Xu, Rui Chen, Shanshan Qi, Xiang Liu, Beibei Lin, Xiaohua Chen, Xiaoying Zhang, Lijuan Yue, Chen Chen","doi":"10.1080/10520295.2023.2287452","DOIUrl":"10.1080/10520295.2023.2287452","url":null,"abstract":"We investigated possible protective effects of chlorogenic acid (CGA) against cyclophosphamide (CP) induced hepatic injury in mice. We measured aminotransferase alanine transaminase (ALT) and aspartate transaminase (AST) levels in the serum. We assayed catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in hepatic tissue. We assessed expression of nuclear transcription factor 2 (Nrf2) and Kelch sample related protein-1 (keap1) proteins in hepatic tissues using immunohistochemistry. The relative mRNA expression levels of heme oxygenase-1 (HO-1), NADH quinone oxidoreductase 1 (NQO1), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Hematoxylin & eosin staining was used to assess liver histopathology. We found that administration of CGA prior to induction of injury by CP decreased serum ALT, AST and MDA expressions in hepatic tissue, while CAT, SOD, GSH and GSH-Px concentrations were increased. We found that hepatocytes of animals administered CGA gradually returned to normal morphology. CGA increased the protein expression of Nrf2 in murine hepatic tissue. Administration of CGA up-regulated mRNA expression levels of HO-1, NQO1, TNF-α and IL-6 in hepatic tissue. CGA exhibited a marked protective effect on CP induced liver injury in mice.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"33-43"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138450826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of licorice root (Glycyrrhiza glabra) extract on the livers of obese rats. 甘草提取物对肥胖大鼠肝脏的影响

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-10-01 Epub Date: 2024-10-15 DOI: 10.1080/10520295.2024.2401159

Ahmet Uyar, Barış Özdere, Turan Yaman, Ahmet Ufuk Kömüroğlu

{"title":"Effects of licorice root (Glycyrrhiza glabra) extract on the livers of obese rats.","authors":"Ahmet Uyar, Barış Özdere, Turan Yaman, Ahmet Ufuk Kömüroğlu","doi":"10.1080/10520295.2024.2401159","DOIUrl":"10.1080/10520295.2024.2401159","url":null,"abstract":"We have investigated anti-obesity effects of the extract of licorice (Glycyrrhiza glabra) root in rats with diet-induced obesity and hyperlipidemia by using histopathological and biochemical methods. Thirty-two Wistar albino rats were divided to four groups of eight: normal control (C), high fat diet (HFD), high fat Diet + Glycyrrhiza glabra (HFD+M), and normal diet with Glycyrrhiza glabra (M). The high fat diet contained 300 g/kg fat (4000 kcal/kg); the daily dosage of Glycyrrhiza glabra extract was 1g/kg body weight by orogastric gavage. Supplementation of Glycyrrhiza glabra extract dramatically reduced increases in body weight caused by the induction of obesity. A hepatoprotective effect of Glycyrrhiza glabra extract was supported by the almost normal histology in the livers of the HFD+M rats, in contrast to the degenerative changes in the HFD rats, which included macrovesicular and microvesicular fat deposits, hydropic degeneration, dilatation of sinusoids and coagulation necrosis of some hepatocytes. Serum levels of alanine transaminase (ALT), aspartic transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), cholesterol (HDL and LDL) and triglycerides, were ameliorated by Glycyrrhiza glabra extract treatment. We conclude that Glycyrrhiza glabra extract given together with HFD could prevent obesity and reduce liver damage in rats.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"370-378"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0