Chelsea Peeler, Christopher R Pitzer, Hector G Paez, Sheila Criswell
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引用次数: 0
摘要
由于福尔马林等大多数化学固定剂的毒性和健康风险,在解剖病理实验室中使用福尔马林等化学固定剂需要接受安全培训和危险化学品监测。因此,在欧洲,福尔马林已被禁止用于大多数用途;主要的例外是在组织学实验室中使用福尔马林,以替代更安全的合适替代品。乙二醛溶液是最有前途的替代固定剂,因为它们的固定机理与福尔马林相似。但与福尔马林不同的是,乙二醛溶液不会从水中解离,因此不需要通风橱等通风措施。采用市售乙二醛溶液的一个主要障碍是其 pH 值较低,可能会产生不良的形态学和抗原性组织变化;不过,最近开发出了一种中性 pH 值的乙二醛产品(无乙二醛酸)(GAF),可以缓解 pH 值低带来的挑战。我们比较了用 10%中性缓冲福尔马林、市售酸性乙二醛产品(Prefer)和 GAF 固定的组织的形态和组织化学。用福尔马林和 Prefer 固定的组织显示出相似的形态和染色特性;而用 2% GAF 固定的组织则显示出有害影响。
Histochemical and morphological evaluation of a glyoxal acid-free fixative.
The application of most chemical fixatives, such as formalin, in the anatomic pathology laboratory requires safety training and hazardous chemical monitoring due to the toxicity and health risks associated with their use. Consequently, the use of formalin has been banned in most applications in Europe; the primary exception is its use in the histology laboratory in lieu of a suitable and safer alternative. Glyoxal based solutions, several of which are available commercially, are the most promising alternative fixatives, because they are based on a mechanism of fixation similar to that of formalin. Unlike formalin, however, glyoxal based solutions do not dissociate from water and therefore do not require ventilation measures such as a fume hood. A primary barrier to the adoption of commercially available glyoxal based solutions is their low pH, which can produce undesirable morphological and antigenic tissue alterations; however, a recently available neutral pH glyoxal product (glyoxal acid free) (GAF) has been developed to mitigate the challenges of low pH. We compared the morphology and histochemistry among tissues fixed in 10% neutral buffered formalin, a commercially available acidic glyoxal product (Prefer), and GAF. Tissues fixed in formalin and Prefer exhibited similar morphology and staining properties; tissues fixed with 2% GAF exhibited deleterious effects.
期刊介绍:
Biotechnic & Histochemistry (formerly Stain technology) is the
official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926.
Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well.
Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.