Biotechnic & Histochemistry最新文献

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Grape seed oil attenuates sodium arsenite-induced gastric, hepatic and colonic damage in Wistar rats. 葡萄籽油减轻亚砷酸钠诱发的 Wistar 大鼠胃、肝和结肠损伤。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-08 DOI: 10.1080/10520295.2024.2426049
Akinleye Stephen Akinrinde, Stephen Oluwasemiloore Oyewole, Abimbola Olumide Adekanmbi
{"title":"Grape seed oil attenuates sodium arsenite-induced gastric, hepatic and colonic damage in Wistar rats.","authors":"Akinleye Stephen Akinrinde, Stephen Oluwasemiloore Oyewole, Abimbola Olumide Adekanmbi","doi":"10.1080/10520295.2024.2426049","DOIUrl":"https://doi.org/10.1080/10520295.2024.2426049","url":null,"abstract":"<p><p>Arsenic exposure is associated with numerous morbidities due to dysfunction of various organ systems including the gastrointestinal tract. We investigated the protective effect of grape seed oil (GSO) against sodium arsenite (NaAsO<sub>2</sub>)-induced gastric, hepatic and colonic injuries in rats. Twenty-four male Wistar rats were divided into four groups of six as follows: Group A (control) received saline; group B received NaAsO<sub>2</sub> (2.5 mg/kg) orally for 7 days; group C were treated concurrently with NaAsO<sub>2</sub> and GSO (2 ml/kg), while group D received only GSO. Administration of NaAsO<sub>2</sub> induced significant (<i>p</i> < 0.05) increases in alanine aminotransferase (ALT) and aspartate aminotransferase (AST); increased periodic acid Schiff (PAS) staining for mucus and increased goblet cell numbers in the stomach and colon; inflammatory cell infiltration and vascular congestion and alterations in the fecal bacterial flora. GSO supplementation generally promoted a reversal of changes induced by NaAsO<sub>2</sub> towards control levels. Additionally, there was increased immunohistochemically detected expression of colonic B-cell lymphoma-1 (Bcl-2) and cytokeratins AE1/AE3, but reduced expression of mucin 1 (MUC1) and carcinoembryonic antigen (CEA) in NaAsO<sub>2</sub> + GSO and GSO treated rats when compared with the NaAsO<sub>2</sub> group. These results suggest that GSO promoted anti-inflammatory processes in the liver, stomach and colon, as well as opposing apoptosis in the colon, resulting in significant attenuation of damage to these tissues.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the protective effects of silymarin and thymoquinone in the focal cerebral ischemia-reperfusion rat model. 比较水飞蓟素和胸腺醌在局灶性脑缺血再灌注大鼠模型中的保护作用
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-07 DOI: 10.1080/10520295.2024.2421511
Merve Solmaz, Ender Erdogan, Dervis Dasdelen, Rasim Mogulkoc, Husamettin Vatansev, Fikret Akyurek, Hanefi Ozbek
{"title":"Comparison of the protective effects of silymarin and thymoquinone in the focal cerebral ischemia-reperfusion rat model.","authors":"Merve Solmaz, Ender Erdogan, Dervis Dasdelen, Rasim Mogulkoc, Husamettin Vatansev, Fikret Akyurek, Hanefi Ozbek","doi":"10.1080/10520295.2024.2421511","DOIUrl":"https://doi.org/10.1080/10520295.2024.2421511","url":null,"abstract":"<p><p>Silymarin and thymoquinone exert neuroprotective effects, although their combined effects in focal cerebral ischemia/reperfusion (I/R) models are unknown. We compared the effect of silymarin and thymoquinone in an I/R rat model. Wistar rats were divided into five groups: SHAM, REP (I/R), SIR (200 mg/kg silymarin+I/R), TIR (3 mg/kg thymoquinone+I/R), and STIR (200 mg/kg silymarin+3-mg thymoquinone+I/R). The rats underwent bilateral carotid artery occlusion for 30 min and neurological assessments 24 h thereafter. Apoptosis was evaluated using anti-caspase-3 and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assays. Astrocyte activation was determined using an anti-GFAP antibody. Total antioxidant status (TAS), total oxidant status (TOS), and trimethylamine N-oxide (TMAO) levels were measured. SHAM and REP rats had the lowest and highest neurological scores, respectively (p = 0.001). REP rats showed greater deterioration than SIR, TIR, and STIR rats. SIR, TIR, and STIR rats had fewer TUNEL and caspase-3-positive cells than REP rats (p<0.05). GFAP expression was higher in REP rats (p<0.05) than in SIR, TIR, and STIR rats (p<0.05). SIR and TIR rats showed higher TAS than REP rats (p<0.05). SIR, TIR, and STIR rats had lower TMAO values than REP and SHAM rats (p<0.05). Silymarin/thymoquinone reduces impairment, apoptosis, and astrocyte activation. Combination therapy reduces TMAO levels.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-18"},"PeriodicalIF":1.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of irisin and exercise on adropin and betatrophin in a new metabolic syndrome model. 在一种新的代谢综合征模型中,鸢尾素和运动对adropin和betatropin的影响。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2023.2276205
Suna Aydin, Faruk Kilinc, Kader Ugur, Mustafa Ata Aydin, Mehmet Hanifi Yalcin, Tuncay Kuloglu, Nalan Kaya Tektemur, Serdal Albayrak, Elif Emre, Meltem Yardim, Ramazan Fazil Akkoc, Serhat Hancer, İbrahim Sahin, Vedat Cinar, Taner Akbulut, Selcuk Demircan, Bahri Evren, Berrin Tarakci Gencer, Aziz Aksoy, Merve Yilmaz Bozoglan, İsa Aydemir, Suleyman Aydin
{"title":"Effects of irisin and exercise on adropin and betatrophin in a new metabolic syndrome model.","authors":"Suna Aydin, Faruk Kilinc, Kader Ugur, Mustafa Ata Aydin, Mehmet Hanifi Yalcin, Tuncay Kuloglu, Nalan Kaya Tektemur, Serdal Albayrak, Elif Emre, Meltem Yardim, Ramazan Fazil Akkoc, Serhat Hancer, İbrahim Sahin, Vedat Cinar, Taner Akbulut, Selcuk Demircan, Bahri Evren, Berrin Tarakci Gencer, Aziz Aksoy, Merve Yilmaz Bozoglan, İsa Aydemir, Suleyman Aydin","doi":"10.1080/10520295.2023.2276205","DOIUrl":"10.1080/10520295.2023.2276205","url":null,"abstract":"<p><p>Metabolic syndrome (MetS) is a prevalent public health problem. Uric acid (UA) is increased by MetS. We investigated whether administration of UA and 10% fructose (F) would accelerate MetS formation and we also determined the effects of irisin and exercise. We used seven groups of rats. Group 1 (control); group 2 (sham); group 3 (10% F); group 4 (1% UA); group 5 (2% UA); group 6 (10% F + 1% UA); and Group 7, (10% F + 2% UA). After induction of MetS (groups 3 -7), Group 3 was divided into three subgroups: 3A, no further treatment; 3B, irisin treatment; 3C, irisin treatment + exercise. Group 4, 1% UA, which was divided into three subgroups: 4A, no further treatment; 4B, irisin treatment; 4C, Irisin treatment + exercise. Group 5, 2% UA, which was divided into three subgroups: 5A, no further treatment; 5B, irisin treatment; 5C, irisin treatment + exercise. Group 6, 10% F + 1% UA, which was divided into three subgroups: 6A, no further treatment; 6B, irisin treatment; 6C, irisin treatment + exercise. Group 7, 10% F + 2% UA, which was divided into three subgroups: 7A, no further treatment; 7B, irisin treatment; 7C, irisin treatment + exercise., İrisin was administered 10 ng/kg irisin intraperitoneally on Monday, Wednesday, Friday, Sunday each week for 1 month. The exercise animals (in addition to irisin treatment) also were run on a treadmill for 45 min on Monday, Wednesday, Friday, Sunday each week for 1 month. The rats were sacrificed and samples of liver, heart, kidney, pancreas, skeletal muscles and blood were obtained. The amounts of adropin (ADR) and betatrophin in the tissue supernatant and blood were measured using an ELISA method. Immunohistochemistry was used to detect ADR and betatrophin expression in situ in tissue samples. The duration of these experiments varied from 3 and 10 weeks. The order of development of MetS was: group 7, 3 weeks; group 6, 4 weeks; group 5, 6 weeks; group 4, 7 weeks; group 3, 10 weeks. Kidney, liver, heart, pancreas and skeletal muscle tissues are sources of adropin and betatrophin. In these tissues and in the circulation, adropin was decreased significantly, while betatrophin was increased significantly due to MetS; irisin + exercise reversed this situation. We found that the best method for creating a MetS model was F + UA2 supplementation. Our method is rapid and simple. Irisin + exercise was best for preventing MetS.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"21-32"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The number and distribution of proliferating cells in the rat's rostral migratory stream as identified by means of two different proliferation markers. 用两种不同的增殖标记物鉴定大鼠喙迁徙流中增殖细胞的数量和分布。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-01 DOI: 10.1080/10520295.2024.2424252
Kamila Fabianová, Adam Raček, Alexandra Popovičová, Marcela Martončíková, Enikő Račeková
{"title":"The number and distribution of proliferating cells in the rat's rostral migratory stream as identified by means of two different proliferation markers.","authors":"Kamila Fabianová, Adam Raček, Alexandra Popovičová, Marcela Martončíková, Enikő Račeková","doi":"10.1080/10520295.2024.2424252","DOIUrl":"https://doi.org/10.1080/10520295.2024.2424252","url":null,"abstract":"<p><p>In the brains of adult rodents, the cells arising in the subventricular zone of the lateral ventricles maintain the ability to divide when migrating to the olfactory bulb along the rostral migratory stream (RMS). Dividing cells in the RMS are most frequently revealed through immunohistochemical detection of an exogenous marker of proliferation, 5-Bromo-2-deoxyuridine (BrdU), which incorporates into DNA during the S-phase of mitosis. The more recently recognized antigen Ki-67 (also known as Kiel-67 and MKI67), an endogenous protein expressed in nuclei at all stages of mitosis, is also used for proliferation detection. BrdU and Ki-67 are often used as alternative methods, but they have not previously been compared in the RMS. We analyzed the numbers and distribution of cells labeled either with BrdU or Ki-67 within the RMS of adult rats. The first group of animals received a single i.p. dose of BrdU. In the second group, dividing cells were visualized by Ki-67 immunohistochemistry. Some sections from brains of BrdU-treated rats were also immunostained for Ki-67. Labeled cells were counted in the three anatomical parts of the RMS (vertical arm, elbow and horizontal arm) using a method for unbiased estimation of cell density. The distribution of proliferating cells was similar for both markers. Most BrdU and Ki-67 positive cells were located in the vertical arm and in the elbow, but a caudo-rostral reduction in cell divisions was more evident with Ki-67 labeling. The number of Ki-67 positive cells significantly exceeded the number of BrdU positive cells in all parts of the RMS. Our results indicate that BrdU and Ki-67 are not interchangeable markers for evaluation of proliferative activity in the RMS.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histochemical and morphological evaluation of a glyoxal acid-free fixative. 无乙二醛酸固定液的组织化学和形态学评估
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2023.2300797
Chelsea Peeler, Christopher R Pitzer, Hector G Paez, Sheila Criswell
{"title":"Histochemical and morphological evaluation of a glyoxal acid-free fixative.","authors":"Chelsea Peeler, Christopher R Pitzer, Hector G Paez, Sheila Criswell","doi":"10.1080/10520295.2023.2300797","DOIUrl":"10.1080/10520295.2023.2300797","url":null,"abstract":"<p><p>The application of most chemical fixatives, such as formalin, in the anatomic pathology laboratory requires safety training and hazardous chemical monitoring due to the toxicity and health risks associated with their use. Consequently, the use of formalin has been banned in most applications in Europe; the primary exception is its use in the histology laboratory in lieu of a suitable and safer alternative. Glyoxal based solutions, several of which are available commercially, are the most promising alternative fixatives, because they are based on a mechanism of fixation similar to that of formalin. Unlike formalin, however, glyoxal based solutions do not dissociate from water and therefore do not require ventilation measures such as a fume hood. A primary barrier to the adoption of commercially available glyoxal based solutions is their low pH, which can produce undesirable morphological and antigenic tissue alterations; however, a recently available neutral pH glyoxal product (glyoxal acid free) (GAF) has been developed to mitigate the challenges of low pH. We compared the morphology and histochemistry among tissues fixed in 10% neutral buffered formalin, a commercially available acidic glyoxal product (Prefer), and GAF. Tissues fixed in formalin and Prefer exhibited similar morphology and staining properties; tissues fixed with 2% GAF exhibited deleterious effects.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"49-58"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Romanowsky staining: history, recent advances and future prospects from a chemistry perspective. 罗曼诺夫斯基染色:从化学角度看历史、最新进展和未来前景。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2023.2273860
Valeriy Kalinin, Pavel Padnya, Ivan Stoikov
{"title":"Romanowsky staining: history, recent advances and future prospects from a chemistry perspective.","authors":"Valeriy Kalinin, Pavel Padnya, Ivan Stoikov","doi":"10.1080/10520295.2023.2273860","DOIUrl":"10.1080/10520295.2023.2273860","url":null,"abstract":"<p><p>Romanowsky staining was an important methodological breakthrough in diagnostic hematology and cytopathology during the late 19<sup>th</sup> and early 20<sup>th</sup> centuries; it has facilitated for decades the work of biologists, hematologists and pathologists working with blood cells. Despite more than a century of studying Romanowsky staining, no systematic review has been published that explains the chemical processes that produce the \"Romanowsky effect\" or \"Romanowsky-Giemsa effect\" (RGE), i.e., a purple coloration arising from the interaction of an azure dye with eosin and not due merely to their simultaneous presence. Our review is an attempt to build a bridge between chemists and biomedical scientists and to summarize the available data on methylene blue (MB) demethylation as well as the related reduction and decomposition of MB to simpler compounds by both light and enzyme systems and microorganisms. To do this, we analyze modern data on the mechanisms of MB demethylation both in the presence of acids and bases and by disproportionation due to the action of light. We also offer an explanation for why the RGE occurs only when azure B, or to a lesser extent, azure A is present by applying experimental and calculated physicochemical parameters including dye-DNA binding constants and electron density distributions in the molecules of these ligands. Finally, we discuss modern techniques for obtaining new varieties of Romanowsky dyes by modifying previously known ones. We hope that our critical literature study will help scientists understand better the chemical and physicochemical processes and mechanisms of cell staining with such dyes.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-20"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stains recently certified. 污渍最近通过了认证。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2024.2286747
{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2024.2286747","DOIUrl":"https://doi.org/10.1080/10520295.2024.2286747","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"99 1","pages":"59"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective effects of chlorogenic acid against cyclophosphamide induced liver injury in mice. 绿原酸对环磷酰胺所致小鼠肝损伤的保护作用。
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2023.2287452
Hao Hao, Youmei Xu, Rui Chen, Shanshan Qi, Xiang Liu, Beibei Lin, Xiaohua Chen, Xiaoying Zhang, Lijuan Yue, Chen Chen
{"title":"Protective effects of chlorogenic acid against cyclophosphamide induced liver injury in mice.","authors":"Hao Hao, Youmei Xu, Rui Chen, Shanshan Qi, Xiang Liu, Beibei Lin, Xiaohua Chen, Xiaoying Zhang, Lijuan Yue, Chen Chen","doi":"10.1080/10520295.2023.2287452","DOIUrl":"10.1080/10520295.2023.2287452","url":null,"abstract":"<p><p>We investigated possible protective effects of chlorogenic acid (CGA) against cyclophosphamide (CP) induced hepatic injury in mice. We measured aminotransferase alanine transaminase (ALT) and aspartate transaminase (AST) levels in the serum. We assayed catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in hepatic tissue. We assessed expression of nuclear transcription factor 2 (Nrf2) and Kelch sample related protein-1 (keap1) proteins in hepatic tissues using immunohistochemistry. The relative mRNA expression levels of heme oxygenase-1 (HO-1), NADH quinone oxidoreductase 1 (NQO1), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined using quantitative real-time polymerase chain reaction (qRT-PCR). Hematoxylin & eosin staining was used to assess liver histopathology. We found that administration of CGA prior to induction of injury by CP decreased serum ALT, AST and MDA expressions in hepatic tissue, while CAT, SOD, GSH and GSH-Px concentrations were increased. We found that hepatocytes of animals administered CGA gradually returned to normal morphology. CGA increased the protein expression of Nrf2 in murine hepatic tissue. Administration of CGA up-regulated mRNA expression levels of <i>HO-1</i>, <i>NQO1</i>, <i>TNF-α</i> and <i>IL-6</i> in hepatic tissue. CGA exhibited a marked protective effect on CP induced liver injury in mice.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"33-43"},"PeriodicalIF":1.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138450826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Therapeutic effect of berberine against 5-fluorouracil induced ovarian toxicity in rats. 小檗碱对 5-氟尿嘧啶诱导的大鼠卵巢毒性的治疗作用
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-10-23 DOI: 10.1080/10520295.2024.2415005
Selim Demir, Nihal Turkmen Alemdar, Hatice Kucuk, Elif Ayazoglu Demir, Ahmet Menteşe, Yuksel Aliyazıcıoğlu
{"title":"Therapeutic effect of berberine against 5-fluorouracil induced ovarian toxicity in rats.","authors":"Selim Demir, Nihal Turkmen Alemdar, Hatice Kucuk, Elif Ayazoglu Demir, Ahmet Menteşe, Yuksel Aliyazıcıoğlu","doi":"10.1080/10520295.2024.2415005","DOIUrl":"https://doi.org/10.1080/10520295.2024.2415005","url":null,"abstract":"<p><p>Berberine (BER) is a naturally occurring alkaloid with a multitude of beneficial effects on human health. Although it is one of the most studied phytochemicals, its curative effect against ovarian damage caused by 5-fluorouracil (5-FU) has not been demonstrated to date. The aim of this study was to investigate the possible protective effect of BER against 5-FU-induced ovotoxicity, focusing on its ability to attenuate oxidative stress, inflammation and apoptosis. The 30 female rats were randomly divided into five groups: Control, BER (2 mg/kg), 5-FU (100 mg/kg), 5-FU+BER (1 mg/kg) and 5-FU+BER (2 mg/kg). The levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and caspase-3 were determined using spectrophotometric methods. In addition, ovarian samples were evaluated histopathologically using hematoxylin&eosin staining method. The MDA, TOS, 8-OHdG, IL-6, TNF-α and caspase-3 levels significantly increased by 5-FU administration. Also, we found that 5-FU significantly decreased TAS, SOD and CAT levels. Treatments with BER significantly attenuated the 5-FU-induced ovarian damage via increasing the antioxidant capacity and reducing the oxidative stress, inflammation and apoptosis in a dose-dependent manner. Moreover, the ovoprotective effect of BER was also confirmed by histopathological evaluation. BER may be evaluated as a potential candidate molecule to reduce 5-FU-induced ovarian toxicity.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-7"},"PeriodicalIF":1.6,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of licorice root (Glycyrrhiza glabra) extract on the livers of obese rats. 甘草提取物对肥胖大鼠肝脏的影响
IF 1.6 4区 生物学
Biotechnic & Histochemistry Pub Date : 2024-10-15 DOI: 10.1080/10520295.2024.2401159
Ahmet Uyar, Barış Özdere, Turan Yaman, Ahmet Ufuk Kömüroğlu
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