Biotechnic & Histochemistry最新文献

Cross-reactivity of anti-human antibodies in canine tissues using immunohistochemistry. 免疫组织化学研究犬组织中抗人抗体的交叉反应性。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-10-08 DOI: 10.1080/10520295.2025.2566683

Abbey Forehand, Sheila Criswell

{"title":"Cross-reactivity of anti-human antibodies in canine tissues using immunohistochemistry.","authors":"Abbey Forehand, Sheila Criswell","doi":"10.1080/10520295.2025.2566683","DOIUrl":"https://doi.org/10.1080/10520295.2025.2566683","url":null,"abstract":"Dogs represent the most common domesticated animal outside of the research arena for whom tissue for pathological diagnosis is requested. Currently, there is a paucity of literature describing the cross-reactivity of anti-human antibodies on canine tissue for oncological diagnosis. This study aimed to evaluate the cross-reactivity of commonly used anti-human diagnostic antibodies in veterinary histopathology. One hundred seventy-one various samples from 153 canine tissues were processed to paraffin and tested with 76 fully validated anti-human antibody clones frequently employed in hospital pathology laboratories using immunohistochemistry methods to determine which ones exhibited comparable cross-reactivity and therefore potential use in veterinary pathology laboratories. Some of the anti-human antibodies were excluded from the study due to a lack of comparable canine tissues on which to test. However, almost half of the antibodies demonstrated results similar to those in human tissues and about one quarter showed weaker or less consistent labeling. The antibodies that demonstrated weaker or inconsistent labeling suggest areas where further development and modification could improve their employment on canine specimens. Fully one-third of antibodies tested failed to show labeling congruent with human tissues. The outcomes from this study could facilitate the adoption of established anti-human antibody markers in veterinary histopathology practices.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-8"},"PeriodicalIF":1.4,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-10-08 DOI: 10.1080/10520295.2025.2565887

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-10-07 DOI: 10.1080/10520295.2025.2565886

引用次数: 0

Investigation of ameliorative effects of indole-3 carbinol on TNBS-induced ulcerative colitis in rats. 吲哚-3甲醇对tnbs诱导的大鼠溃疡性结肠炎的改善作用研究。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-10-03 DOI: 10.1080/10520295.2025.2561682

Erkmen Tuğrul Epikmen, Emrah İpek, Mehmet Hesapçıoğlu, Mehmet Karaboğa, Ali Riza Öztürk, Hamdi Avci

{"title":"Investigation of ameliorative effects of indole-3 carbinol on TNBS-induced ulcerative colitis in rats.","authors":"Erkmen Tuğrul Epikmen, Emrah İpek, Mehmet Hesapçıoğlu, Mehmet Karaboğa, Ali Riza Öztürk, Hamdi Avci","doi":"10.1080/10520295.2025.2561682","DOIUrl":"https://doi.org/10.1080/10520295.2025.2561682","url":null,"abstract":"Ulcerative colitis is a chronic inflammatory condition of the gastrointestinal tract that can predispose patients to colonic neoplasms. Various natural compounds have been explored for their therapeutic potential. Indole-3-carbinol (I3C), a natural compound derived from cruciferous vegetables, is recognized for its tissue-protective and regenerative properties. This study aimed to investigate the effects of I3C on experimental ulcerative colitis in rats. Thirty-two Wistar rats were randomly assigned to four groups: a control group receiving isotonic saline, a TNBS group administered trinitrobenzene sulfonic acid (TNBS) intrarectally, an I3C group receiving I3C via gastric gavage, and a TNBS+I3C group treated with I3C following TNBS induction. After 7 days, all animals were euthanized under anesthesia, and pathological, histochemical, and immunohistochemical evaluations were conducted. The results revealed that I3C mitigated the severity of TNBS-induced colonic lesions and facilitated tissue repair. The I3C-treated group exhibited reduced tissue damage and enhanced mucosal regeneration. Additionally, vessel count, collagen, and myofibroblastic activity were markedly increased following I3C treatment. In conclusion, I3C exhibits both protective and reparative effects in experimental ulcerative colitis, potentially through anti-inflammatory mechanisms and the activation of tissue repair pathways.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-11"},"PeriodicalIF":1.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative role of curcumin and ascorbic acid on nephrotoxicity induced by environmentally relevant concentrations of different combinations of lead, fluoride and nitrate in albino rats. 姜黄素和抗坏血酸对环境相关浓度铅、氟化物和硝酸盐不同组合所致白化大鼠肾毒性的改善作用

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-09-29 DOI: 10.1080/10520295.2025.2555991

Chetanjyoti Tuteja, Navdeep Kaur

{"title":"Ameliorative role of curcumin and ascorbic acid on nephrotoxicity induced by environmentally relevant concentrations of different combinations of lead, fluoride and nitrate in albino rats.","authors":"Chetanjyoti Tuteja, Navdeep Kaur","doi":"10.1080/10520295.2025.2555991","DOIUrl":"https://doi.org/10.1080/10520295.2025.2555991","url":null,"abstract":"Groundwater pollution with lead, fluoride, and nitrate presents a growing environmental and health challenge. This study aimed to evaluate the nephrotoxic effects of these pollutants in male albino rats and assess the potential ameliorative effects of curcumin and ascorbic acid in counteracting their toxicity for 135 days. A total of ten treatment groups were established viz. control, lead + fluoride + nitrate (BIS), lead + nitrate, lead + nitrate + curcumin + ascorbic acid, lead + fluoride, lead + fluoride + curcumin + ascorbic acid, fluoride + nitrate, fluoride + nitrate + curcumin + ascorbic acid, lead + fluoride + nitrate, lead + fluoride + nitrate + curcumin + ascorbic acid. Exposure to lead, fluoride, and nitrate resulted in a significant decrease in the activity of oxidative stress enzymes viz. superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase, and a notable increase in the lipid peroxidation levels. Further, significantly increased urea and creatinine levels in plasma and renal damage including glomerular shrinkage, widened Bowman's space, and tubular degeneration were also observed. The greatest damage was recorded in the lead + fluoride + nitrate group followed by lead + fluoride, lead + nitrate, and fluoride + nitrate. Co-treatment with curcumin and ascorbic acid demonstrated remarkable protective effects, with improvements in oxidative stress markers, plasma urea, and creatinine levels along with a significant restoration of glomerular structure and normalization of Bowman's space reflecting improved renal function. This research highlights the kidneys' susceptibility to environmental toxicants and the combined efficacy of curcumin and ascorbic acid in mitigating nephrotoxicity.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":1.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of acetyl-CoA carboxylase induces ferroptosis and inhibits tumor growth in oral squamous cell carcinoma. 下调乙酰辅酶a羧化酶诱导铁下垂并抑制口腔鳞状细胞癌肿瘤生长。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-09-12 DOI: 10.1080/10520295.2025.2555561

Zhen Xu, Guodong Jia

{"title":"Downregulation of acetyl-CoA carboxylase induces ferroptosis and inhibits tumor growth in oral squamous cell carcinoma.","authors":"Zhen Xu, Guodong Jia","doi":"10.1080/10520295.2025.2555561","DOIUrl":"https://doi.org/10.1080/10520295.2025.2555561","url":null,"abstract":"In this study, we aimed to investigate the specific role of the acetyl-CoA carboxylase (ACACA) gene in oral squamous cell carcinoma (OSCC). We constructed the human tongue carcinoma cell line SAS with low ACACA expression and evaluated changes in cell proliferation, apoptosis, and ferroptosis. Then, the effect of combined treatment with cisplatin and ferroptosis inducer erastin was measured. To assess the impact of ACACA expression on tumor growth in vivo, we established xenograft models with varying ACACA levels in twelve male BALB/c nude mice. ACACA knockdown significantly reduced the proliferation ability of SAS cells, and increased the number of apoptotic cells. ACACA knockdown also induces ferroptosis, and this effect was enhanced by combined treatment with cisplatin and erastin. In vivo experiments demonstrated lower tumor volume and weight in the ACACA knockdown group than those in the control group. Exploring the combined effect of ACACA knockdown and cisplatin treatment revealed a promising synergistic effect against ferroptosis signaling and downstream signaling pathways in SAS cells and in vivo. These findings suggest that targeting the ACACA gene has the potential to be a novel therapeutic strategy for oral cancer treatment.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DAPI (4',6-diamidino-2-phenylindole) as a novel fluorochrome for amyloid staining that binds to β-pleated sheet. DAPI（4'，6-二氨基-2-苯基吲哚）作为淀粉样蛋白染色的一种新型荧光染料，与β-褶片结合。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-09-09 DOI: 10.1080/10520295.2025.2552251

Yu Uchida, Mao Mizukawa, Yumiko Kamiya, Takanori Shiga, Naoyuki Aihara, Junichi Kamiie

{"title":"DAPI (4',6-diamidino-2-phenylindole) as a novel fluorochrome for amyloid staining that binds to β-pleated sheet.","authors":"Yu Uchida, Mao Mizukawa, Yumiko Kamiya, Takanori Shiga, Naoyuki Aihara, Junichi Kamiie","doi":"10.1080/10520295.2025.2552251","DOIUrl":"https://doi.org/10.1080/10520295.2025.2552251","url":null,"abstract":"Amyloidosis is caused by the extracellular deposition of amyloid fibrils with a β-pleated sheet structure. Diagnosis typically relies on Congo red or Thioflavine T staining. Recently, DAPI (4',6-Diamidino-2-Phenylindole), which is a common nuclear fluorochrome, has been reported to stain amyloid. DAPI staining is simpler than Congo Red and Thioflavine T staining, but its staining mechanism remains unclear. Thus, this study aimed to investigate the mechanism and specificity of DAPI staining for amyloid and its utility. The staining properties of DAPI and Thioflavine T for amyloid were compared on the basis of their stereochemical similarity. In addition, formic acid-treated amyloid specimens were used to investigate the mechanism by which structural changes affect DAPI binding to amyloid fibrils. DAPI staining was also evaluated in four specimens with different types of amyloid. DAPI and Thioflavine T had similar stereochemistry and staining behavior. The amyloid present in formic acid-treated specimens was negative for DAPI staining, indicating that DAPI may recognize the conformation of amyloid fibrils. DAPI stained positively in specimens deposited with AA, Aβ, AL, and AIAPP. DAPI staining recognizes the β-pleated sheet structure of the amyloid fibril structure, and is a simple and sensitive method for detecting amyloid deposition.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":1.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff's reagent in periodic acid-Schiff staining: Its application to in-resin correlative light-electron microscopy. 7-氨基-4-甲基香豆素在周期性酸-希夫染色中作为希夫试剂的荧光替代品：在树脂相关光电子显微镜中的应用。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-09-01 DOI: 10.1080/10520295.2025.2548790

Hiroshi Takase, Daisuke Hachisuka, Tomoya Sawano, Mariko Sugiura, Keiichiro Fujii, Ayako Masaki, Takayuki Murase, Hiroshi Inagaki

{"title":"7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff's reagent in periodic acid-Schiff staining: Its application to in-resin correlative light-electron microscopy.","authors":"Hiroshi Takase, Daisuke Hachisuka, Tomoya Sawano, Mariko Sugiura, Keiichiro Fujii, Ayako Masaki, Takayuki Murase, Hiroshi Inagaki","doi":"10.1080/10520295.2025.2548790","DOIUrl":"https://doi.org/10.1080/10520295.2025.2548790","url":null,"abstract":"Periodic acid-Schiff (PAS) staining is useful for visualizing glycogen and mucus. 7-amino-4-methylcoumarin (AMC), an organic reagent, exhibits blue fluorescent signals upon UV excitation. We recently showed that AMC can be used as a fluorescent substitute for Schiff's reagent in PAS staining. In-resin correlative light-electron microscopy (CLEM) is an excellent technique employing fluorescent and electron microscopy (EM) images obtained from the same resin-embedded ultra-thin sections, and provides a high level of morphological concordance between the fluorescent and EM images. Here we studied whether AMC was useful in detecting PAS-positive cells in in-resin CLEM using human pathological samples. Formalin-fixed, paraffin-embedded sections of human colonic and renal tissues and colonic amoebiasis were used. After staining with AMC, these samples were subjected to EM preparation and embedded in epoxy resin. Semi-thin and ultra-thin sections were prepared, and fluorescent and EM images were obtained. AMC signals were well preserved in colonic goblet cells, renal basement membrane, and amoebic bodies in epoxy resin-embedded sections. Using the CLEM technique, a small number of amoebic bodies were easily detected in an inflammatory background of colonic amoebiasis. In conclusion, we successfully detected PAS-positive cells in in-resin CLEM with AMC. Our method may enhance EM analysis in human pathology.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-7"},"PeriodicalIF":1.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression profiles of Nrf1 and Nrf2 in adult rat small intestine. Nrf1和Nrf2在成年大鼠小肠中的表达谱。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-09-01 DOI: 10.1080/10520295.2025.2550471

Füsun Erhan Baycumendur, Elif Nur Tas Kepenek

{"title":"Expression profiles of Nrf1 and Nrf2 in adult rat small intestine.","authors":"Füsun Erhan Baycumendur, Elif Nur Tas Kepenek","doi":"10.1080/10520295.2025.2550471","DOIUrl":"https://doi.org/10.1080/10520295.2025.2550471","url":null,"abstract":"Safeguarding the integrity and functionality of the gastrointestinal system is paramount, given its vulnerability to several detrimental effects. One of the factors that can cause functional disorders is oxidative stress, which can disrupt the homeostasis of intestinal tissue and cause various diseases. In this study, we aimed to evaluate the expression patterns of nuclear factor erythroid 2-like 1 (Nrf1) and nuclear factor erythroid 2-like 2 (Nrf2) transcription factors, which are part of an important cleaning system that protects cells against oxidative stress, in the adult rat small intestine by immunohistochemical means. Six Wistar albino adult rats were used in this study. After taking 5 μm thick sections of the small intestine, immunohistochemistry labeling was performed to investigate the expression of Nrf1 and Nrf2. Both transcription factors were found to exhibit immunopositivity in the duodenum, jejunum, and ileum segments of the small intestine. In the crypt epithelium, Nrf1 and Nrf2 showed similar intensity and distribution of staining, whereas, in the villus epithelium, Nrf2 immunoreactivity was most prominent in the ileum and weakest in the jejunum. Nrf1 exhibited lower staining intensity in the ileum. In the smooth muscle layers and the myenteric plexus, Nrf2 immunopositivity was highest in the duodenum, while it was weaker in the jejunum and ileum. These findings indicate that Nrf1 and Nrf2 display region-specific expression patterns in the small intestine and suggest that these transcription factors may play distinct roles in the regional oxidative stress response of intestinal tissues.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-10"},"PeriodicalIF":1.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopic and ultrastructural insights into the protective role of melatonin against tartrazine-induced hepatotoxicity. 褪黑素对酒石黄诱导的肝毒性的保护作用的微观和超微结构见解。

IF 1.4 4区生物学

Biotechnic & Histochemistry Pub Date : 2025-08-27 DOI: 10.1080/10520295.2025.2548792

Esraa M Hussein, Nora F Ghanem, Samaa M Bakr, Shaimaa M Kasem, Mohamed A Dkhil, Felwa A Thagfan, Amina E Essawy

{"title":"Microscopic and ultrastructural insights into the protective role of melatonin against tartrazine-induced hepatotoxicity.","authors":"Esraa M Hussein, Nora F Ghanem, Samaa M Bakr, Shaimaa M Kasem, Mohamed A Dkhil, Felwa A Thagfan, Amina E Essawy","doi":"10.1080/10520295.2025.2548792","DOIUrl":"https://doi.org/10.1080/10520295.2025.2548792","url":null,"abstract":"Tartrazine, a coal tar-derived azo color, is utilized in food, drinks, cosmetics, and pharmaceuticals. Its azo group catabolizes in the gut, poisoning the liver. This study investigated the efficacy of melatonin, an endogenous antioxidant from the pineal gland against hepatotoxicity in tartrazine-intoxicated rats. Thirty-two adult male wistar albino rats were allocated into four groups: control group, melatonin group (10 mg/kg), tartrazine-treated group (7.5 mg/kg), and tartrazine + melatonin-treated group (7.5 mg/kg tartrazine + 10 mg/kg melatonin). Doses were taken daily for 4 weeks. Melatonin's influence on hepatotoxicity was assessed by monitoring liver enzyme activity, antioxidant state, apoptotic and inflammatory markers, DNA fragmentation, histological and ultrastructural changes. Rats exposed to tartrazine exhibited elevated liver enzymes, oxidant-antioxidant imbalance, and elevated hepatic inflammatory markers (TNF-α, IL-6). Tartrazine also damaged DNA and induced histological and ultrastructural alterations in liver tissue, as shown by the comet assay. Alpha-fetoprotein (AFP) and proliferating cell nuclear antigen (PCNA) were strongly expressed in immunohistochemistry. In rats, melatonin significantly reduced all tartrazine effects. Conversely, melatonin treatment significantly alleviated all aforementioned effects induced by tartrazine in rats by decreasing liver enzymes, elevating antioxidant enzymes, and reducing hepatic inflammatory markers. Enhanced histological assessment and the ultrastructure of the liver was detected following melatonin use. The use of melatonin may safeguard against tartrazine-induced hepatic DNA damage. In conclusion, the current findings indicate that tartrazine administration has detrimental health effects and deleterious impacts on liver function and structure. Melatonin mitigated tartrazine-induced liver damage via antioxidant, anti-inflammatory, and anti-apoptotic pathways.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":1.4,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144941582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0