Biotechnic & Histochemistry最新文献

{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2026.2669446","DOIUrl":"https://doi.org/10.1080/10520295.2026.2669446","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1"},"PeriodicalIF":1.4,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2026.2669445","DOIUrl":"https://doi.org/10.1080/10520295.2026.2669445","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1"},"PeriodicalIF":1.4,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2026.2669447","DOIUrl":"https://doi.org/10.1080/10520295.2026.2669447","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1"},"PeriodicalIF":1.4,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of therapeutic efficacy of intraperitoneal ketamine administration on the brain vascular system in rats with traumatic brain injury.","authors":"Tunahan Yapici, Beste Mentese, M Y Pekmezci, Nurbanu Aygunduz Yapici, Necip Kutlu, Mesut Mete, Elgin Turkoz Uluer, M Kemal Ozbilgin","doi":"10.1080/10520295.2026.2645058","DOIUrl":"10.1080/10520295.2026.2645058","url":null,"abstract":"<p><p>Traumatic brain injury (TBI) is a condition characterized by high morbidity and mortality. This study investigated the therapeutic effects of ketamine on TBI through electrophysiological and immunohistochemical methods in a model of 30 rats divided into five equal groups. Following trauma, ketamine, mannitol, and hypertonic saline treatments were administered. Analyses conducted on the 10th day post-trauma revealed that ketamine most effectively reduced the expressions of HMGB1, S100b, RAGE, and EGR-1 across all treatment groups. Furthermore, EEG findings demonstrated a significant decrease in Delta/Alpha and Delta/Beta ratios in the ketamine group. These results suggest that ketamine exerts a healing effect on brain tissue by suppressing pathological delta waves and modulating the HMGB1/S100b/RAGE/EGR-1 signaling pathway.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"226-239"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diaminobenzidine: 60 years of use in the life sciences, and still going strong.","authors":"Christopher J von Ruhland","doi":"10.1080/10520295.2026.2649494","DOIUrl":"10.1080/10520295.2026.2649494","url":null,"abstract":"<p><p>Since the introduction of diaminobenzidine (DAB) by Richard Graham and Morris Karnovsky 60 years ago, it has enjoyed remarkable success in the life sciences. In this review, the chemistry, reactivity, and polymerization of DAB, and speculations around the coordination chemistry of its polymer, polyDAB, are discussed, and notable developments in the use of DAB in the life sciences, since a previous review in 1979, are described. Histochemical applications include the localization of hydrogen peroxide in plant tissues that accumulates as a consequence of pathology, the metal-catalyzed polymerization of DAB for visualizing endogenous metal complexes, for increasing the visibility of histochemical deposits such as Perl's stain, and for localizing metal oxide nanoparticles. In enzyme histochemistry, DAB is still used for demonstrating cytochrome oxidases activity, but its polymerization by exogenously applied horseradish peroxidase (HRP) is where it gained particular prominence both in neuronal tracing, and especially in immunohistochemistry. The physical inhibition of enzymatic activity, following deposition of polyDAB, that limited immunohistochemical sensitivity led to a variety of methods to address this issue. These exploited the ability of polyDAB to form complexes with metals, resulting in an increase in its intensity and sometimes also its color, its capacity to be amplified by silver-based development, or both. The alteration in the color of polyDAB was also employed for multiple sequential immunostaining with HRP or when immunohistochemistry was combined with other histochemical techniques. More recently, DAB has found utility in the ultrastructural localization of specific proteins that have been genetically labeled with peroxidase tags or fluorochromes, the latter being visualized by the process of photoconversion which exploits the fact that fluorescence excitation can be coupled to the polymerization of DAB. Finally, the future of DAB in the life sciences, whether in its native form or some modified version, is speculated on.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"254-273"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk between autophagy and matrix remodeling pathways in the synovial tissue of estrogen-deficient and diabetic rats.","authors":"R Florencio-Silva, G R S Sasso, P C Franco, R S Simões, M J Simões","doi":"10.1080/10520295.2026.2654453","DOIUrl":"10.1080/10520295.2026.2654453","url":null,"abstract":"<p><p>Estrogen deficiency and diabetes mellitus (DM) are major endocrine and metabolic disorders contributing to musculoskeletal degeneration through oxidative stress, inflammation, and extracellular matrix remodeling. The synovial membrane, essential for joint homeostasis, is particularly vulnerable to these systemic disturbances. Autophagy, a key cellular mechanism for maintaining synovial integrity by degrading damaged organelles and proteins, may be dysregulated under such conditions; however, its regulation in response to estrogen deficiency and DM remains poorly understood. This study investigated the combined effects of estrogen deficiency and streptozotocin-induced DM on rat knee synovial tissue, focusing on histopathological changes and the immunoexpression of autophagy (Beclin-1, LC3B), angiogenic (VEGF-A), matrix remodeling (MMP-9), and inflammasome-related (NLRP3) markers. Twenty adult female Wistar rats were ovariectomized (OVX) or SHAM-operated (SHAM) and assigned to SHAM, OVX, SHAM-DM, and OVX-DM groups. DM was induced by intraperitoneal streptozotocin injection in the diabetic groups. After seven weeks, knee joints were fixed in paraformaldehyde, decalcified, and embedded in paraffin. Sections were analyzed histologically and immunohistochemically. OVX-DM rats showed pronounced synovial lining hyperplasia, fibrosis, and vascular proliferation, with increased expression of Beclin-1, LC3B, MMP-9, VEGF-A, and NLRP3. Significant positive correlations were observed among these markers and with fibrosis, vascularity, and inflammation scores. These findings suggest that estrogen deficiency and DM synergistically promote pathological synovial remodeling via activation of autophagic, angiogenic, and matrix degradation pathways.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"274-287"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined melatonin and curcumin treatment ameliorates renal ischemia-reperfusion injury via reducing oxidative stress, inflammation, and apoptosis in rats.","authors":"S Topkaraoglu, T Sapmaz, K Sevgin, M Tekayev, S Kuras, H H Pence, E M Guler, S Aktas, O Irkorucu","doi":"10.1080/10520295.2026.2642383","DOIUrl":"10.1080/10520295.2026.2642383","url":null,"abstract":"<p><p>Renal ischemia-reperfusion (Isc-Rep) injury commonly occurs during kidney transplantation, renal vascular surgery, or traumatic hemorrhagic shock, and is marked by excessive oxidative stress and inflammation. We aimed to examine the antioxidant properties of the melatonin-curcumin combination in an experimental renal Isc-Rep model in rats. Thirty-five rats were allocated into five distinct experimental groups. While the rats in the Control (C) group did not undergo any surgical procedure, each of the rats in the remaining groups underwent 45 minutes of renal ischemia followed by 2 h of reperfusion. In all experimental groups, except the Isc-Rep group, the rats were administered an active compound immediately prior to reperfusion, namely melatonin (MEL, 20 mg/kg), curcumin (CUR, 200 mg/kg), or a combination of MEL-CUR. In all groups, histological evaluation was carried out, while apoptotic cell analyses were similarly performed by means of the TUNEL method. In addition, oxidative stress parameters (TOS, TAS, OSI), inflammatory cytokines (TNF-α, IL-6, and IL-1β), superoxide-dismutase (SOD), and malondialdehyde (MDA) activity levels were also examined. Histological evaluation demonstrated that renal Isc-Rep-induced cellular damage, tubular dilatation, and inflammation were significantly reduced in the MEL-CUR groups in comparison with the Isc-Rep group (p < 0.005). Furthermore, statistically significant differences were observed among the groups in TOS, TAS, OSI, TNF-α, IL-6, and IL-1β levels (p < 0.005). The co-administration of melatonin and curcumin resulted in greater protection against ischemia-reperfusion-induced renal damage compared with either treatment alone. Moreover, the co-administration of these antioxidant agents demonstrates greater therapeutic efficacy compared to their individua.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"215-225"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neuroprotective effects of thalidomide in cerebral ischemia-reperfusion injury induced by bilateral carotid artery occlusion in rats.","authors":"Onural Ozhan, Ugur Cem Mete, Azibe Yildiz, Merve Durhan, Nigar Vardi, Yilmaz Cigremis, Gul Busra Kaya, Ahmet Acet, Hakan Parlakpinar","doi":"10.1080/10520295.2026.2648550","DOIUrl":"10.1080/10520295.2026.2648550","url":null,"abstract":"<p><p>This study investigates the neuroprotective effects of thalidomide (THA) in cerebral ischemia-reperfusion (I/R) injury induced by bilateral carotid artery occlusion in rats. Thirty-two female Wistar albino rats were divided into four groups: Sham, I/R, THA+I/R, and I/R+THA. I/R injury was induced by occluding both carotid arteries for 15 minutes followed by 24-hour reperfusion. THA (20 mg/kg) was administered intraperitoneally either before or after ischemia. Biochemical analyses measured malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in brain tissues. Histopathological evaluations assessed neuronal degeneration and tissue damage. The I/R group exhibited significantly increased MDA levels and reduced SOD and GSH enzyme activities in cerebrum and cerebellum tissues (P < 0.05). THA treatment significantly decreased MDA levels and restored SOD and GSH enzyme activities in both THA+I/R and I/R+THA groups (P < 0.05). Histopathological analyses revealed neuronal degeneration in the I/R group, whereas THA treatment mitigated neuronal damage, particularly in the I/R+THA group. In the IR+THA group, there was a significant decrease in the severity of caspase-3 immunoreactivity compared to the I/R group. THA demonstrates neuroprotective effects against cerebral I/R injury by reducing oxidative stress and neuronal damage. Administration of THA post-ischemia appears to be more effective in preserving neuronal integrity. These findings suggest that THA has neuroprotective potential in an experimental model of cerebral I/R injury; however, given its known adverse effects, further studies focusing on safety, dosing, and risk-benefit balance are required before any clinical implications can be considered.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"240-253"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of omega-3 supplements against ocular surface damage in circadian disruption rat model.","authors":"Mehmet Argun, Semra Acer, Özlem Özmen, Cafer Açıkgöz, Özlem Tök","doi":"10.1080/10520295.2026.2661626","DOIUrl":"https://doi.org/10.1080/10520295.2026.2661626","url":null,"abstract":"<p><p>Circadian rhythms are biological processes that occur in approximately 24-hour cycles in organisms. Disruption of circadian rhythms (CD) is thought to have adverse effects on many organs, including the eyes. The aim of this study was to evaluate the effects of circadian disruption on the cornea and conjunctiva, as well as the impact of two forms of omega-3 supplementation on the ocular surface, using a rat model of circadian disruption. This experimental study included 32 female Wistar albino rats, which were divided into four groups. The control group (Group 1) was maintained under normal feeding and sleeping conditions without any disruption. Circadian disruption was induced in the other three groups, which were administered saline (Group 2), fish oil (Group 3), or flaxseed oil (Group 4) via oral gavage. On the 31st day, all rats were euthanized, and corneal, conjunctival, and palpebral tissues were collected from both eyes through enucleation. Histological examination and immunohistochemical analysis of Caspase-3 (Cas-3), tumor necrosis factor-alpha (TNF-α), and PERIOD-2 (PER-2) were performed on the corneal and conjunctival tissues. Group 2 exhibited significantly thicker corneas compared to Group 1 (P < 0.001). Additionally, hyperemia, inflammatory cell infiltration in the conjunctiva, higher expression levels of Cas-3 and TNF-α, and decreased PER-2 expression were observed in the corneal and conjunctival tissues of Group 2. These pathological changes were minimal or absent in Groups 3 and 4. Notably, Group 3 showed better amelioration of these alterations compared to Group 4. Disruption of circadian rhythms can have a negative impact on the cornea and conjunctiva. Omega-3 supplementation demonstrated a significant protective effect against ocular tissue damage induced by circadian disruption. Fish oil was more effective than flaxseed oil in reducing corneal thickening, inflammation, and apoptotic marker expression, highlighting its potential as a therapeutic intervention for circadian rhythm-related ocular pathologies.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of fluorescent biomarkers for determination of effects induced by cyanobacterial oligopeptides: microginin-FR1, anabaenopeptin-B, aeruginosins 98A and 98B in RTgill-W1 cells.","authors":"Adam Bownik, Barbara Pawlik-Skowrońska","doi":"10.1080/10520295.2026.2661621","DOIUrl":"https://doi.org/10.1080/10520295.2026.2661621","url":null,"abstract":"<p><p>The aim of this study was to determine the effects of three linear cyanobacterial oligopeptides: microginin-FR1 (MG-FR1), anabaenopeptin-B (ANA-B), aeruginosin 98A (AER-A), aeruginosin 98B (AER-B) and one cyclic oligopeptide anabaenopeptin-B (ANA-B) on RTgill-W1 fluorescent biomarkers of cell line derived from <i>Rainbow trout</i> gill cells. The following cellular parameters were determined after 48-h exposure to different concentrations (205 nM, 512 nM and 1004 nM) of the oligopeptides: Hoechst and Propidium Iodide (PI) fluorescence, intracellular glutathione level and cytoskeletal F-actin structure. The study showed that AER-B and MG-FR1 were the most potent inhibitors of Hoechst fluorescence, AER-B additionally increased PI fluorescence level. MG-FR1 and ANA-B substantially reduced glutathione and F-actin fluorescence level in RTgill-W1 cells. The obtained results suggest that both linear and cyanobacterial oligopeptides should be considered as factors inducing detrimental alterations in RTgill-W1 cells, suggesting that in natural conditions exposure of fish to cyanobacterial metabolites may result in cytotoxic effects in fish gills.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":1.4,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}