Biotechnic & Histochemistry最新文献

{"title":"Postradiation angiosarcoma of the breast: a 25-year single-institution analysis of clinicopathological characteristics and immunohistochemical biomarker study.","authors":"Kristin J Rybski, Brian S Finkelman, Bin Zhang, Ajay Dhakal, Bradley M Turner, David G Hicks, Huina Zhang","doi":"10.1080/10520295.2026.2626268","DOIUrl":"10.1080/10520295.2026.2626268","url":null,"abstract":"<p><p>We examined the clinicopathologic features and immunohistochemical expression of commonly tested biomarkers relevant to targeted therapy in postradiation angiosarcoma (PRAS) of the breast, aiming to better understand tumor biology and provide baseline data on tumor biomarkers to inform future therapeutic strategies for PRAS. Clinicopathologic features, outcomes, and the immunohistochemical expression of tumor biomarkers, including human epidermal growth factor receptor 2 (HER2), programmed death-ligand 1 (PD-L1), and DNA mismatch repair proteins (MMR), were analyzed in breast PRAS specimens diagnosed from 2000 to 2024. A total of 24 breast PRAS specimens from 18 patients were included in the study. All PRAS patients were female, with a median age of 72 years at the time of diagnosis. High-grade tumors were observed in 47%, 43%, and 65% of specimens according to Fédération Nationale des Centers de Lutte Contre le Cancer (FNCLCC), Donnell-Rosen, and Kuba scoring methods, respectively. Surgical intervention, adjuvant chemotherapy, and radiotherapy were administered to 100%, 29%, and 23% of patients, respectively. Five patients (29%) died from the disease during a median follow-up period of 37 months. Exploratory analysis showed there was no significant association between histologic grade and overall survival, regardless of the grading system used. All PRASs were MMR-proficient with a low number of tumor-infiltrating lymphocytes (TILs) and exhibited an absence of HER2 protein expression. PD-L1 was positive in seven (32%) specimens and positively correlated with stromal TILs. Our findings suggest that breast PRAS are genomically stable and poorly immunogenic, and are likely ineligible for any HER2-targeted therapies based on current approval criteria. A subset of specimens demonstrated PD-L1 positivity, underscoring the need for further investigation of immunotherapy with anti-PD1/PD-L1 immune checkpoint inhibitors as a potential treatment option.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"173-183"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hsa-miR-10a-5p/RNF186 modulates endoplasmic reticulum stress to exacerbate the development of ulcerative colitis.","authors":"Yifan Guo, Haidong Wu, Xuyong Chen, Fan Jiang, Ye Zhao, Xinpu Miao","doi":"10.1080/10520295.2025.2606089","DOIUrl":"10.1080/10520295.2025.2606089","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a chronic inflammatory bowel disease, and endoplasmic reticulum stress (ERS) may contribute to the pathogenesis and progression of UC. Previous research has found that hsa-miR-10a-5p is abnormally expressed in UC, but its molecular mechanisms remain unclear. This study aimed to explore the role of hsa-miR-10a-5p in regulating ERS in UC through RNF186. LPS-induced HT-29 cells and a dextran sulfate sodium (DSS)-induced animal model were utilized to explore the regulatory roles of hsa-miR-10a-5p and RNF186 in ERS in UC. Following modulation of hsa-miR-10a-5p and RNF186 expression, we assessed the expression of ERS-related genes in the UC model using qPCR and immunofluorescence, and evaluated apoptosis with flow cytometry and WB. Furthermore, we conducted DAI scoring, HE staining, and permeability testing in the animal model, and analyzed the inflammatory profile of UC by ELISA to further understand disease progression and the impact of molecular changes on intestinal pathology. Overexpression of hsa-miR-10a-5p in HT-29 cells and animal models promoted cell proliferation, inhibited apoptosis, and mitigated inflammatory factor release and ERS response. Conversely, miR-10a-5p knockdown activated colonic mucosal epithelial damage, reduced cell proliferation, increased apoptosis, aggravated ERS response, and enhanced inflammatory factor expression. This research elucidated that hsa-miR-10a-5p participates in the process of UC development and progression by modulating the ERS response through RNF186. This discovery offered novel insights, enhancing our comprehension of the underlying pathophysiology of UC and provided a theoretical basis for the potential application of miRNAs in UC therapy.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"145-160"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145932045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical labeling of carbonic anhydrase isoenzymes in labial glands of infants.","authors":"Mechthild Stoeckelhuber, Klaus-Dietrich Wolff, Marco R Kesting, Denys J Loeffelbein, Christoph Schmitz, Lucas M Ritschl","doi":"10.1080/10520295.2026.2618820","DOIUrl":"10.1080/10520295.2026.2618820","url":null,"abstract":"<p><p>In salivary glands, the concurrent availability of HCO3^- is required for normal mucus release that is guaranteed by the carbonic anhydrases in this area. Due to lack of detailed carbonic anhydrase data in minor salivary glands, we identified various carbonic anhydrases (CA) in the human labial glands of infants in this study. Specifically, the CA isoenzymes II, III, IV, VI, VII, and XII were investigated in secretory and ductal epithelial cells. CA II was detected in serous glandular cells and sporadically in ductal cells, CA III in myoepithelial cells and skeletal muscle cells, CA IV in ductal cells, endothelial cells, erythrocytes, and skeletal muscle. The first immunohistochemical analysis of CA VII in salivary glands resulted in a positive reaction in serous glandular cells and ductal cells, as well as in skeletal muscle and endothelial cells. CA XII was sporadically localized at the basolateral membrane of ductal cells and in serous glandular cells. Mucous endpieces were negative in all carbonic anhydrases tested. Knowledge of carbonic anhydrases distribution in healthy tissues supports their assessment as biomarkers for cancer diagnosis, prevention, and therapy.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"207-213"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the protective effects of tannic acid against bleomycin-induced lung fibrosis in rats.","authors":"Amir Siahpoosh, Narges Atefipour, Mohammadreza Momeni Qomi, Mehdi Goudarzi, Laya Sadat Khorsandi, Rezvan Hafezi, Ali Reza Malayeri","doi":"10.1080/10520295.2025.2611824","DOIUrl":"10.1080/10520295.2025.2611824","url":null,"abstract":"<p><p>Pulmonary fibrosis (PF) is a prevalent adverse effect associated with bleomycin (Bleo) administration. Despite extensive research efforts, there is no effective treatment currently available for PF. The current study investigates the antifibrotic and antioxidant properties of tannic acid (TA), a natural polyphenol known for its potent antioxidant and anti-inflammatory activities, in the context of Bleo-induced PF in rats. Fifty Wistar rats were randomly assigned to five distinct groups for the study. Group 1 served as the control (normal saline). Group 2 received a single dose of Bleo (7.5 U/kg via intratracheal injection) on the 7th day. Groups 3, 4, and 5 were treated with 100, 200, and 400 mg/kg of TA, respectively, administered 7 days prior to and 21 days following Bleo administration. The rats were euthanized 24 hours after the last administration. The protective effects of TA were assessed through histopathological and biochemical analyses, which included measurements of lung index, hydroxyproline (HP), nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). Additionally, the influence of TA on tumor necrosis factor-α (TNF-α) levels was evaluated. The results indicated that TA significantly reduced the Bleo-induced increase in lung index, MDA, NO, HP, and TNF-α levels. Furthermore, TA enhanced the activities of CAT, SOD, and GPx, as well as the content of GSH. Additionally, TA mitigated the infiltration of fibroblasts and inflammatory cells and prevented alveolar thickening induced by Bleo. The findings demonstrated that TA exhibits a protective effect against PF induced by Bleo.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"161-172"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and evaluation of an in-situ hybridization assay for infectious pancreatic necrosis virus.","authors":"Nihat Toplu, Kubilay Metin, Harun Albayrak, Tuba Çiğdem Oğuzoğlu, Erkemen Tuğrul Epikmen, Emrah İpek, Ayşe Nur Akkoç","doi":"10.1080/10520295.2026.2640836","DOIUrl":"10.1080/10520295.2026.2640836","url":null,"abstract":"<p><p>In the present study, an in-situ hybridization (ISH) assay was performed on cell lines and paraffin-embedded tissue blocks infected with infectious pancreatic necrosis (IPN) virus in fish. The molecular diagnosis by ISH was established through four main steps: (1) generation of DIG (Digoxigenin)-labeled probes, (2) production of anti-DIG antibodies and their conjugation with alkaline phosphatase, (3) preparation of ISH reagents, including pre-hybridization and hybridization solutions, and (4) application of the ISH assay to cell culture and paraffin tissue sections. Protease concentrations, hybridization solutions, and assay modifications were optimized separately for cell lines and paraffin sections. Ultimately, ISH assays using DIG-labeled probes targeting the VP2 and VP3 gene regions of IPN virus were successfully performed in virus-infected cell lines and paraffin-embedded tissues.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"184-194"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the expressions of GPR30, GPR120 and GPR125 proteins in rat testis in the experimentally created Parkinson model.","authors":"S Ünver Saraydin, M Ergin","doi":"10.1080/10520295.2026.2641746","DOIUrl":"10.1080/10520295.2026.2641746","url":null,"abstract":"<p><p>Parkinson's disease (PD) is a progressive neurodegenerative disorder associated with aging. It is characterized by both motor and non-motor symptoms, including cognitive impairment, mood disturbances, sleep disruption, sexual dysfunction, and weight loss. These symptoms may precede a clinical diagnosis. Sex-related differences in PD, influenced by estrogen's neuroprotective effects and testosterone deficiency, contribute to a higher prevalence and severity of the disease in males. Male rats received daily subcutaneous injections of rotenone (2 mg/kg) for 20 days to establish a PD model, which enabled the investigation of central dopaminergic degeneration and peripheral testicular effects. Tyrosine hydroxylase (TH) immunoreactivity in the substantia nigra pars compacta (SNpc) was assessed alongside the testicular expression of GPR30, GPR120, and GPR125 in the testes using immunohistochemical and immunofluorescence techniques. Rotenone-treated animals exhibited significant weight loss (z = -4.292, <i>p</i> < 0.001), reduced TH expression in the SNpc, and hallmark PD pathology including Lewy body-like inclusions, Lewy neurites, and halo-like structures around dopaminergic neuron nuclei. Testicular analysis revealed a time-dependent decline in GPR protein expression. GPR30 was found in spermatids and interstitial cells, GPR120 was found predominantly in Leydig cells, and GPR125 was found in interstitial tissue, Sertoli cells, and spermatocytes. These findings suggest that the loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) may affect testicular function via testosterone-dependent regulation of GPR30, GPR120, and GPR125 expression. This highlights the systemic nature of PD and its potential impact on male reproductive health. Alterations in testicular G Protein-Coupled Receptors (GPCRs) may serve as peripheral biomarkers of disease progression, emphasizing the importance of considering endocrine and reproductive dysfunction in both experimental models and the clinical management of PD.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"195-206"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling mechanistic effects of mast cell in the progression of fibrosis and malignant transformation of oral submucous fibrosis: a systematic review and meta-analysis.","authors":"Keerthika R, Akhilesh Chandra, Dinesh Raja, Rahul Agarwal","doi":"10.1080/10520295.2025.2595966","DOIUrl":"10.1080/10520295.2025.2595966","url":null,"abstract":"<p><p>Oral submucous fibrosis (OSMF) is a ubiquitous fatal fibrotic mucosal disease with multifactorial etiology and complex pathogenesis. The role of mast cells in the pathophysiology of OSMF remains uncharted territory owing to a dearth of studies. Thus, the present systematic review and meta-analysis aimed to unentangle the mysteric role of mast cells in the pathogenesis, progression of fibrosis and malignant transformation of OSMF. Using various databases, full-text articles that investigated mast cell concentrations in OSMF were entailed for review. A modified Newcastle-Ottawa scale was employed to evaluate the risk of bias in all articles and Review Manager was utilized for meta-analysis. Twenty and fourteen qualified articles, respectively, were included for qualitative and quantitative data synthesis. Progressive amplification of mast cell density is linked with fibrosis-induced malignant transformation of OSMF. The fixed-effect model also confirmed that significantly upregulated mast cell counts have a decreased risk of association with control as well as a significantly increased risk of being associated with early-stage fibrosis and malignant transformation of OSMF. This review authenticates the mechanistic effects of mast cells in the pathogenesis, chronicity, progression of fibrosis and malignant transformation of OSMF.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"109-118"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic and ultrastructural insights into the nephro-therapeutic role of chitosan nanoparticles against streptozotocin-induced diabetic nephropathy.","authors":"Aml A Elngar, Eman A Moussa, Felwa A Thagfan, Huda A Alqahtani, Reem Dkhil, Nabila M Mira, Shaimaa M Kasem, Mohamed A Dkhil, Nora F Ghanem","doi":"10.1080/10520295.2025.2603933","DOIUrl":"10.1080/10520295.2025.2603933","url":null,"abstract":"<p><p>Diabetes mellitus is a prevalent chronic disease, with diabetic nephropathy being a significant complication that causes structural alterations in the kidneys. The purpose of this research was to examine the effects of nano-chitosan (NCh) on the kidneys of rats with diabetes induced by streptozotocin (STZ). NCh was prepared and characterized using scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy technique (EDX) and zeta potential. A total of 40 male Sprague Dawley albino rats (225 ± 25 grams, 2.5-3 months old) were involved and divided into 4 equal groups, each with 10 rats: Control (Ctrl), non-diabetic-administered with 0.5 mg/kg B.W NCh (NCh), diabetic induced by STZ intraperitoneally (Diabetic), and diabetic treated with NCh at 0.5 mg/kg B.W (Diabetic-NCh). Seven days post-diabetic induction, NCh was inoculated orally for 21 days once a day. Body weight change and kidney function tests (creatinine and urea), as well as renal histopathological, histochemical (collagen fibers), immunohistochemical; alpha smooth muscle actin (αSMA) and transforming growth factor-beta1 (TGF-β1), and ultrastructural studies were involved. The findings indicated that diabetic-NCh treated rats exhibited improvements in body weight and kidney function tests including creatinine and urea, compared to the diabetic rats. NCh treatment enhanced renal tissue architecture and reduced collagen fiber expression. Immunohistochemical analysis showed decreased concentrations of αSMA and TGF-β1 in the Diabetic-NCh group. Ultrastructural studies confirmed the improvement in kidney tissue structure. In conclusion, oral administration of nano-chitosan demonstrated a potential therapeutic effect on kidneys in diabetic rats, suggesting its promise as a treatment strategy for diabetic nephropathy.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"119-132"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiapoptotic effects of anthocyanin on testicular damage induced by varicocele in male rats.","authors":"Ghasem Rostami, Mohammad Reza Navabakhsh, Seyedeh Sara Salami, Mohammad Rezaei, Elham Hasannezhad, Alireza Rahimi Mamaghani, Majid Shokoohi, Linda Mohammadzadeh Boukani","doi":"10.1080/10520295.2025.2595962","DOIUrl":"10.1080/10520295.2025.2595962","url":null,"abstract":"<p><p>Varicocele is one of the most important disorders causing infertility in men, and oxidative stress is one of the most important factors affecting testicular parenchyma damage caused by varicocele. This study explored the effect of anthocyanins on varicocele-induced testis injury in adult Wistar rats by focusing on regulating oxidative stress, Bax and Bcl-2 genes, and protein related to cell death. Rats (n = 32) were divided into four groups: Control (Sham), varicocele, varicocele + anthocyanin, and anthocyanin alone. At the end of the study (week 8), the animals were sacrificed, and H&E staining was used for testicular histopathology. The IHC method was used for the detection of Bax and Bcl-2 protein expression, and TUNEL assays were used to analyze testicular Apoptosis. Additionally, serum levels of oxidative stress markers - MDA, SOD, and GPx - were assessed by ELISA, and RT-qPCR analyzed the mRNA expression of Bax and Bcl-2. Histological analysis revealed notable improvements in Johnsen's score, epithelial thickness, and seminiferous tubule diameter in the varicocele + anthocyanin group relative to the varicocele-only group (<i>p </i>< 0.005). Protein and mRNA expression of Bax significantly increased in the varicocele group (<i>p </i>< 0.005), while treatment with anthocyanin enhanced Bcl-2 expression (<i>p </i>< 0.005). Furthermore, the rate of apoptotic positive germ cells decreased when the rats received anthocyanin. Moreover, anthocyanin increased serum levels of GPx and SOD while decreasing MDA levels in the treatment group compared to rats with varicocele (<i>p </i>< 0.005). These outcomes suggest that anthocyanin may moderate testicular injury from varicocele, primarily through its antioxidative properties.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"84-95"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CTRPs, β3-AR signaling, and placental fibrin deposition: molecular and histopathological aspects of preterm birth.","authors":"D Aşkin Özek, R Melekoğlu, N Akpolat, H Yüce, N Zeyveli-Çelik, Ş Yaşar, Y Berberoğlu, K Tanbek, M Hüz, I Ateş, S Ünüvar, S Sandal","doi":"10.1080/10520295.2025.2595963","DOIUrl":"10.1080/10520295.2025.2595963","url":null,"abstract":"<p><p>It has been suggested that adipokines may modulate plasma lipid levels, and β3-adrenergic receptor (β3-AR) gene expressions may affect adipokine levels and play critical roles in lipid metabolism. This study aims to determine predictive biomarkers for preterm birth (PTB) by evaluating serum complement 1q (C1q)/tumor necrosis factor (TNF)-related protein (CTRP) levels, lipid profiles, gene expressions, and placental pathological changes in women experiencing PTB. A total of 80 pregnant women, 40 preterm and 40 term, who applied to the Department of Obstetrics and Gynecology, Faculty of Medicine, Inonu University, were included in the study. Blood and placenta samples were taken from all participants. Serum CTRP, high-density lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol (TC), and triglyceride (TG) levels were measured; β3-AR gene expression and detection rate of the β3-AR <i>rs4994</i> (Trp64Arg) amplicon were evaluated. Placental tissues were examined histopathologically for perivillous/intervillous fibrin deposition and hydropic degeneration. ROC analysis was used to determine predictive biomarkers for PTB. In the PTB group, compared to the control group, β3-AR gene expression levels and serum CTRP3 levels were significantly decreased, while the detection rate of the Trp64Arg amplicon and serum CTRP4 levels were significantly increased. In addition, LDL levels increased significantly (<i>p</i> = 0.046), TC levels decreased (<i>p</i> = 0.045). According to ROC analysis, LDL (<i>p</i> = 0.039), TC (<i>p</i> = 0.034), CTRP3 (<i>p</i> = 0.019), and CTRP4 (<i>p</i> = 0.033) levels were determined as significant predictive biomarkers for PTB. Histopathological examination revealed increased perivillous and intervillous fibrin deposition and marked hydropic degeneration in the PTB group. Changes in CTRP levels, lipid profile disorders, and a decrease in β3-AR signaling pathways were found to be associated with PTB. LDL, TC, CTRP3, and CTRP4 levels can be evaluated as potential biomarkers that can be used in the early diagnosis and management of PTB.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"96-108"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145767177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}