Biotechnic & Histochemistry最新文献

{"title":"Efficiency of griseofulvin and plumbagin in mitigating N-nitrosodiethylamine-induced hepatocellular carcinoma.","authors":"Ekta Tyagi, Rajabrata Bhuyan, Anand Prakash","doi":"10.1080/10520295.2026.2727035","DOIUrl":"https://doi.org/10.1080/10520295.2026.2727035","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) is a globally prevalent malignancy often triggered by oxidative stress and exposure to hepatotoxic agents such as N-nitrosodiethylamine (NDEA), which induces DNA adducts, inflammation, and liver dysfunction. Natural compounds like plumbagin (PL) and griseofulvin (GF) have demonstrated anticancer potential; however, their in vivo efficacy in NDEA-induced HCC models remains insufficiently explored. This study investigated the protective effects of PL and GF against NDEA-induced hepatocarcinogenesis in male Swiss albino mice. Animals were randomly assigned to seven groups, including normal control, NDEA control (200 mg/kg), and treatment groups receiving PL (2 mg/kg and 6 mg/kg, intraperitoneally), GF (225 mg/kg and 450 mg/kg, orally), or the reference drug sorafenib (30 mg/kg, orally) for 14 days. Biochemical, hematological, and oxidative stress markers were evaluated alongside histopathological analysis. NDEA exposure significantly elevated liver enzymes (ALT, AST, ALP), cholesterol, and malondialdehyde (MDA), while reducing antioxidant enzyme levels (SOD, CAT, GST, and GSH-Px), indicating marked hepatic damage. Treatment with PL and GF led to significant, dose-dependent restoration of these parameters, with notable reductions in MDA and improvements in antioxidant activity. Histopathological assessments further confirmed these findings, showing reduced fibrosis, enhanced hepatocyte regeneration, and improved liver architecture, particularly with high-dose PL, which demonstrated efficacy comparable to sorafenib. In conclusion, PL and GF exhibited substantial hepatoprotective and anticancer effects in the NDEA-induced HCC model, primarily by restoring liver function, reducing oxidative stress, and improving histological outcomes. These findings support their potential as complementary therapeutic agents in liver cancer management.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-16"},"PeriodicalIF":1.4,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148944197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoexpression of HSP27 and HSP70 after hyperthermia in postnatal rat lung and heart.","authors":"Banu Kandil, Funda Terzi, Alev Gürol Bayraktaroglu","doi":"10.1080/10520295.2026.2694030","DOIUrl":"10.1080/10520295.2026.2694030","url":null,"abstract":"<p><p>This study aimed to investigate the changes in HSP27 and HSP70 immunoexpression after hyperthermia in the lung and heart of rats on days 5, 10, 20, 25, and 30 of postnatal development. The body temperature of rat pups in the hyperthermia groups was increased to 40°C under the infrared lamp. The recovery time after hyperthermia was kept at 5 hours. HSP27 was present in cardiomyocytes and bronchiole and alveolar epithelial cells of control rats. Compared to the control groups after hyperthermia, HSP27 expression was only significantly different on day PND10 for cardiomyocyte cells (P < 0.05), but not on any day of postnatal development for bronchiole and alveolar epithelial cells (P > 0.05). In control group rats, HSP70 was almost absent in cardiomyocytes and alveolar epithelial cells but was evident in bronchiole epithelial cells. A significant increase in HSP70 expression after hyperthermia was observed throughout the postnatal days in cardiomyocytes and alveolar epithelial cells, but only on day PND20 in bronchiole epithelial cells (P < 0.05). In conclusion, during postnatal development, HSP27 is essential for the development of cardiomyocytes and bronchiole and alveolar epithelial cells, and HSP70 is essential for the development of bronchiole epithelial cells. On the other hand, HSP70 may participate in cytoprotective and repair mechanisms against hyperthermia-induced stress in cardiomyocytes and alveolar epithelial cells during postnatal development.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"341-351"},"PeriodicalIF":1.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148390048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated phenotypic, cytogenetic, and functional characterization of skin fibroblast cultures from the Greater Caribbean manatee (<i>Trichechus manatus manatus</i>, Linnaeus 1758).","authors":"Luanna Lorenna Vieira Rodrigues, Leonardo Vitorino Costa de Aquino, Pedro Rafael Ribeiro Assunção, Yara Letícia Frutuoso E Silva, Vinicius Dantas da Silva, Maria Valéria de Oliveira Santos, Radan Elvis Matias de Oliveira, Fábia Oliveira Luna, Fernanda Loffler Niemeyer Attademo, Alexsandra Fernandes Pereira","doi":"10.1080/10520295.2026.2695350","DOIUrl":"10.1080/10520295.2026.2695350","url":null,"abstract":"<p><p>The Greater Caribbean manatee, recently reclassified as endangered, is a keystone species in coastal and freshwater ecosystems. Its long-term conservation requires integrative strategies that combine <i>in situ</i> and <i>ex situ</i> approaches. Although skin-derived cell cultures have been reported for other sirenian species, none have yet been established and characterized for the Greater Caribbean manatee. Thus, the aim of this study was to establish and characterize skin-derived fibroblast cultures from the Greater Caribbean manatee. Skin biopsies obtained from seven animals were used to establish primary cultures. The cells were expanded <i>in vitro</i>, identified as fibroblasts by vimentin immunostaining, and characterized by ultrastructural features, karyotype stability, and the absence of microbial contamination under antibiotic-free culture conditions. Fibroblasts at passages 2-10 were characterized for morphology, viability, metabolic activity, population doubling time, oxidative stress levels, mitochondrial membrane potential, and apoptotic profile. Explant-derived cultures adhered within 24 h, reached sub-confluence after a mean of 8.4 days, and a full confluence by 17.9 days, sustaining active proliferation for up to 84.6 days across ten passages. Cells displayed typical fibroblast-like morphology, high viability (≥ 84%), and stable metabolic activity and proliferation rates (P > 0.05) throughout the culture period. Reactive oxygen species levels increased significantly at passages 4, 8, and 10 relative to passage 2 (<i>P</i> < 0.05), accompanied by a mild reduction in mitochondrial membrane potential, while overall cellular functionality was preserved. Apoptotic and necrotic events exhibited only transient fluctuations without evidence of progressive cellular damage. Cytogenetic analyses revealed a stable karyotype of 2n = 48, with normal XX/XY constitution and no detectable chromosomal abnormalities. Cultures remained free of microbial contamination for 30 days under antibiotic-free conditions. Therefore, this study establishes the first established fibroblast culture from the Greater Caribbean manatee, offering a novel and reliable platform for functional, genomic, cytogenetic, and toxicological studies in conservation biotechnology.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"370-385"},"PeriodicalIF":1.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between mast cell density and microvascular density with pathological features in canine mammary gland carcinomas.","authors":"Erdinc Guner, Mehmet Karaboğa, Burcu Saraçoğlu","doi":"10.1080/10520295.2026.2694021","DOIUrl":"10.1080/10520295.2026.2694021","url":null,"abstract":"<p><p>Canine mammary gland tumors (CMGTs) are among the most common neoplasms in female dogs, and serve as valuable models for human breast cancer in terms of histopathology and biological behavior. This study aimed to investigate the relationship between mast cell density (MCD) (total, peritumoral, and intratumoral) and microvascular density (MVD), and to determine their association with clinicopathological features in 40 canine mammary carcinomas (CMCs). The mast cells (MCs) assessment was conducted by utilizing standard Giemsa staining. To evaluate angiogenesis, the immunohistochemical expression of CD31 was assessed. Results revealed that peritumoral MCD was significantly higher than intratumoral MCD (P < 0.001). Higher histological grades (Grade III) exhibited increased MVD (P = 0.09) and total/intratumoral MCD (P = 0.031 and P = 0.020, respectively). Necrosis was associated with elevated MVD (P = 0.04) and intratumoral MCD (P = 0.027). However, no correlation was found between MVD and MCD, nor were significant differences observed among histological subtypes and lymphovascular invasion. These findings indicate that mast cells might play a role in tumor progression, potentially by promoting angiogenesis. Additionally, their distribution could serve as an extra histopathological marker that, when combined with traditional grading, may enhance prognostic accuracy for CMCs. Further studies with larger cohorts are needed to highlight the complex role of mast cells in the tumor microenvironment.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"335-340"},"PeriodicalIF":1.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization, and biological impact of PEGylated uricase from Aspergillus flavus: stability, activity, and <i>in vitro</i> immunological evaluation.","authors":"Firouze Mahmoudi, Mohammad Mirzaei, Masoud Torkzadeh-Mahani, Mohamad Mahani","doi":"10.1080/10520295.2026.2694720","DOIUrl":"10.1080/10520295.2026.2694720","url":null,"abstract":"<p><p>Despite the commercial success of protein- and peptide-based therapeutics, their clinical application remains limited due to rapid clearance and inactivation via metabolic pathways. In this study, we PEGylated Aspergillus flavus-derived uricase (Uox) using methoxy polyethylene glycol (5 kDa) activated with Succinimidyl Succinamide (mPEG-SS) at a molar ratio of 1:10 (Uox:mPEG-SS) to enhance its pharmacological properties. This study aimed to evaluate and compare the kinetic behavior, thermodynamic stability, and immunogenic potential of native and PEGylated uricase. mPEG-SS was synthesized through a two-step reaction and characterized by FT-IR,¹ H-NMR spectroscopy, and TLC. Successful conjugation to Uox (mPEG-SS@Uox) was confirmed using DLS and SDS-PAGE. Kinetic and thermodynamic analyses revealed a 16.44% increase in thermal stability and a 23.37% increase in half-life at 45°C upon PEGylation, with only a modest 27.3% reduction in catalytic efficiency, likely due to steric hindrance. Immunogenicity assays using CFSE-labeled PBMC proliferation and cytokine profiling via ELISA indicated that PEGylated Uox induced significantly lower immune activation compared to the native enzyme (p < 0.0045), with no statistical difference from the control group (p = 0.2426). These findings demonstrate that PEGylation with 5 kDa mPEG-SS substantially improves uricase's stability and diminishes its <i>in vitro</i> immunogenic potential (as evidenced by reduced PBMC activation), making it a promising candidate for further preclinical development in the treatment of hyperuricemia and gout.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"352-369"},"PeriodicalIF":1.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148418369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequential multi-organ preparation of rat liver and kidney precision-cut tissue slices: impact of collection method, anatomical region, and processing time on ATP-based viability.","authors":"Almira Fathadina, R H Nawawi, R R Tjandrawinata","doi":"10.1080/10520295.2026.2663024","DOIUrl":"10.1080/10520295.2026.2663024","url":null,"abstract":"<p><p>Precision-cut tissue slices (PCTS), defined as thin, uniformly cut sections of fresh organs that preserve native tissue architecture and cell-cell interactions, serve as valuable ex vivo models for studying tissue physiology, toxicology, and metabolism of drug substances. However, existing PCTS preparation protocols typically focus on single-organ preparation, leaving the feasibility of sequential multi-organ slicing remains uncertain. This study evaluated the viability of rat liver and kidney slices during a 72-hour incubation period, considering factors such as sex, organ collection method, coring technique, and slicing duration. Viability was assessed based on intracellular Adenosine Triphosphate (ATP) as an indicator of metabolic activity. No sex-related differences in ATP levels were observed in 18-20-month-old rats. ATP content was also comparable between central and peripheral liver regions. In contrast, kidney slices composed of the medulla demonstrated significantly higher ATP levels than those predominantly composed of cortex, reflecting regional differences in microanatomy and metabolic adaptability. Furthermore, slices obtained from anesthetized rats showed better ATP recovery during incubation than those from euthanized rats, highlighting the importance of adequate oxygen supply maintained through blood circulation during organ collection. The sequential preparation of liver and kidney slices within 4 hours after organ collection was feasible, as slicing performed within 0-2 hours and 2-4 hours did not significantly affect viability. These findings provide practical insights for optimizing PCTS preparation supporting reliable slice viability, and enabling efficient multi-organ processing when tissue slicer capacity is limited.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"310-323"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147873123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of omega-3 supplements against ocular surface damage in circadian disruption rat model.","authors":"Mehmet Argun, Semra Acer, Özlem Özmen, Cafer Açıkgöz, Özlem Tök","doi":"10.1080/10520295.2026.2661626","DOIUrl":"10.1080/10520295.2026.2661626","url":null,"abstract":"<p><p>Circadian rhythms are biological processes that occur in approximately 24-hour cycles in organisms. Disruption of circadian rhythms (CD) is thought to have adverse effects on many organs, including the eyes. The aim of this study was to evaluate the effects of circadian disruption on the cornea and conjunctiva, as well as the impact of two forms of omega-3 supplementation on the ocular surface, using a rat model of circadian disruption. This experimental study included 32 female Wistar albino rats, which were divided into four groups. The control group (Group 1) was maintained under normal feeding and sleeping conditions without any disruption. Circadian disruption was induced in the other three groups, which were administered saline (Group 2), fish oil (Group 3), or flaxseed oil (Group 4) via oral gavage. On the 31st day, all rats were euthanized, and corneal, conjunctival, and palpebral tissues were collected from both eyes through enucleation. Histological examination and immunohistochemical analysis of Caspase-3 (Cas-3), tumor necrosis factor-alpha (TNF-α), and PERIOD-2 (PER-2) were performed on the corneal and conjunctival tissues. Group 2 exhibited significantly thicker corneas compared to Group 1 (P < 0.001). Additionally, hyperemia, inflammatory cell infiltration in the conjunctiva, higher expression levels of Cas-3 and TNF-α, and decreased PER-2 expression were observed in the corneal and conjunctival tissues of Group 2. These pathological changes were minimal or absent in Groups 3 and 4. Notably, Group 3 showed better amelioration of these alterations compared to Group 4. Disruption of circadian rhythms can have a negative impact on the cornea and conjunctiva. Omega-3 supplementation demonstrated a significant protective effect against ocular tissue damage induced by circadian disruption. Fish oil was more effective than flaxseed oil in reducing corneal thickening, inflammation, and apoptotic marker expression, highlighting its potential as a therapeutic intervention for circadian rhythm-related ocular pathologies.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"301-309"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of fluorescent biomarkers for determination of effects induced by cyanobacterial oligopeptides: microginin-FR1, anabaenopeptin-B, aeruginosins 98A and 98B in RTgill-W1 cells.","authors":"Adam Bownik, Barbara Pawlik-Skowrońska","doi":"10.1080/10520295.2026.2661621","DOIUrl":"10.1080/10520295.2026.2661621","url":null,"abstract":"<p><p>The aim of this study was to determine the effects of three linear cyanobacterial oligopeptides: microginin-FR1 (MG-FR1), anabaenopeptin-B (ANA-B), aeruginosin 98A (AER-A), aeruginosin 98B (AER-B) and one cyclic oligopeptide anabaenopeptin-B (ANA-B) on RTgill-W1 fluorescent biomarkers of cell line derived from <i>Rainbow trout</i> gill cells. The following cellular parameters were determined after 48-h exposure to different concentrations (205 nM, 512 nM and 1004 nM) of the oligopeptides: Hoechst and Propidium Iodide (PI) fluorescence, intracellular glutathione level and cytoskeletal F-actin structure. The study showed that AER-B and MG-FR1 were the most potent inhibitors of Hoechst fluorescence, AER-B additionally increased PI fluorescence level. MG-FR1 and ANA-B substantially reduced glutathione and F-actin fluorescence level in RTgill-W1 cells. The obtained results suggest that both linear and cyanobacterial oligopeptides should be considered as factors inducing detrimental alterations in RTgill-W1 cells, suggesting that in natural conditions exposure of fish to cyanobacterial metabolites may result in cytotoxic effects in fish gills.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"289-300"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xiaokeping combined with low-carbohydrate diet slows cognitive decline in diabetic rats through activating TGF-β1/Smad7 signaling.","authors":"Ying Xun, Xiaoli Wang, Lili Chen, Yu Fu, Xiang Wu, Wenqing Zeng","doi":"10.1080/10520295.2026.2675930","DOIUrl":"10.1080/10520295.2026.2675930","url":null,"abstract":"<p><p>Glucose-lowering treatment and antidiabetic drugs show great potential for preventing and intervening in diabetes mellitus (DM)-associated cognitive dysfunction or advanced dementia. This study delves into the role and mechanism of low-carbohydrate diet (LCD) combined with Xiaokeping (XKP) on these conditions. Type 2 diabetes (T2D) rat models were constructed via high-fat diet and streptozotocin (STZ) injection, and treated with LCD and XKP alone or in combination for 8 weeks. Body weight and indicators of blood glucose and lipids were determined. Additionally, memory and spatial learning abilities were assessed. Tissue staining of the hippocampus was used to observe pathological changes. Oxidative stress, levels of inflammatory factors, and phosphatidylinositol 3-kinase (PI3K)/Akt serine/threonine kinase (Akt) were analyzed. The results demonstrated that LCD and/or XKP treatment recovered body weight, blood glucose, and lipid balance, while enhancing spatial memory and attenuating lesion in the hippocampus of diabetic rats. LCD and/or XKP treatment increased superoxide dismutase, decreased malondialdehyde, interleukin-1β/6, and tumor necrosis factor alpha level in the hippocampus of diabetic rats, which suggests a reduction in oxidative stress and neuroinflammation. Moreover, LCD and/or XKP treatment activated the PI3K/Akt pathway. LCD combined with XKP displayed superior efficacy compared to monotherapy. Collectively, LCD combined with XKP mixture improves cognitive decline of T2D rats by suppressing oxidative stress and neuroinflammation in the hippocampus, through a mechanism involving the activation of the PI3K/Akt pathway.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"324-333"},"PeriodicalIF":1.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2026.2669446","DOIUrl":"https://doi.org/10.1080/10520295.2026.2669446","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1"},"PeriodicalIF":1.4,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}