Biotechnic & Histochemistry最新文献

{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2026.2669445","DOIUrl":"https://doi.org/10.1080/10520295.2026.2669445","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1"},"PeriodicalIF":1.4,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stains recently certified.","authors":"","doi":"10.1080/10520295.2026.2669447","DOIUrl":"https://doi.org/10.1080/10520295.2026.2669447","url":null,"abstract":"","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1"},"PeriodicalIF":1.4,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of therapeutic efficacy of intraperitoneal ketamine administration on the brain vascular system in rats with traumatic brain injury.","authors":"Tunahan Yapici, Beste Mentese, M Y Pekmezci, Nurbanu Aygunduz Yapici, Necip Kutlu, Mesut Mete, Elgin Turkoz Uluer, M Kemal Ozbilgin","doi":"10.1080/10520295.2026.2645058","DOIUrl":"10.1080/10520295.2026.2645058","url":null,"abstract":"<p><p>Traumatic brain injury (TBI) is a condition characterized by high morbidity and mortality. This study investigated the therapeutic effects of ketamine on TBI through electrophysiological and immunohistochemical methods in a model of 30 rats divided into five equal groups. Following trauma, ketamine, mannitol, and hypertonic saline treatments were administered. Analyses conducted on the 10th day post-trauma revealed that ketamine most effectively reduced the expressions of HMGB1, S100b, RAGE, and EGR-1 across all treatment groups. Furthermore, EEG findings demonstrated a significant decrease in Delta/Alpha and Delta/Beta ratios in the ketamine group. These results suggest that ketamine exerts a healing effect on brain tissue by suppressing pathological delta waves and modulating the HMGB1/S100b/RAGE/EGR-1 signaling pathway.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"226-239"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk between autophagy and matrix remodeling pathways in the synovial tissue of estrogen-deficient and diabetic rats.","authors":"R Florencio-Silva, G R S Sasso, P C Franco, R S Simões, M J Simões","doi":"10.1080/10520295.2026.2654453","DOIUrl":"10.1080/10520295.2026.2654453","url":null,"abstract":"<p><p>Estrogen deficiency and diabetes mellitus (DM) are major endocrine and metabolic disorders contributing to musculoskeletal degeneration through oxidative stress, inflammation, and extracellular matrix remodeling. The synovial membrane, essential for joint homeostasis, is particularly vulnerable to these systemic disturbances. Autophagy, a key cellular mechanism for maintaining synovial integrity by degrading damaged organelles and proteins, may be dysregulated under such conditions; however, its regulation in response to estrogen deficiency and DM remains poorly understood. This study investigated the combined effects of estrogen deficiency and streptozotocin-induced DM on rat knee synovial tissue, focusing on histopathological changes and the immunoexpression of autophagy (Beclin-1, LC3B), angiogenic (VEGF-A), matrix remodeling (MMP-9), and inflammasome-related (NLRP3) markers. Twenty adult female Wistar rats were ovariectomized (OVX) or SHAM-operated (SHAM) and assigned to SHAM, OVX, SHAM-DM, and OVX-DM groups. DM was induced by intraperitoneal streptozotocin injection in the diabetic groups. After seven weeks, knee joints were fixed in paraformaldehyde, decalcified, and embedded in paraffin. Sections were analyzed histologically and immunohistochemically. OVX-DM rats showed pronounced synovial lining hyperplasia, fibrosis, and vascular proliferation, with increased expression of Beclin-1, LC3B, MMP-9, VEGF-A, and NLRP3. Significant positive correlations were observed among these markers and with fibrosis, vascularity, and inflammation scores. These findings suggest that estrogen deficiency and DM synergistically promote pathological synovial remodeling via activation of autophagic, angiogenic, and matrix degradation pathways.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"274-287"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147761250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diaminobenzidine: 60 years of use in the life sciences, and still going strong.","authors":"Christopher J von Ruhland","doi":"10.1080/10520295.2026.2649494","DOIUrl":"10.1080/10520295.2026.2649494","url":null,"abstract":"<p><p>Since the introduction of diaminobenzidine (DAB) by Richard Graham and Morris Karnovsky 60 years ago, it has enjoyed remarkable success in the life sciences. In this review, the chemistry, reactivity, and polymerization of DAB, and speculations around the coordination chemistry of its polymer, polyDAB, are discussed, and notable developments in the use of DAB in the life sciences, since a previous review in 1979, are described. Histochemical applications include the localization of hydrogen peroxide in plant tissues that accumulates as a consequence of pathology, the metal-catalyzed polymerization of DAB for visualizing endogenous metal complexes, for increasing the visibility of histochemical deposits such as Perl's stain, and for localizing metal oxide nanoparticles. In enzyme histochemistry, DAB is still used for demonstrating cytochrome oxidases activity, but its polymerization by exogenously applied horseradish peroxidase (HRP) is where it gained particular prominence both in neuronal tracing, and especially in immunohistochemistry. The physical inhibition of enzymatic activity, following deposition of polyDAB, that limited immunohistochemical sensitivity led to a variety of methods to address this issue. These exploited the ability of polyDAB to form complexes with metals, resulting in an increase in its intensity and sometimes also its color, its capacity to be amplified by silver-based development, or both. The alteration in the color of polyDAB was also employed for multiple sequential immunostaining with HRP or when immunohistochemistry was combined with other histochemical techniques. More recently, DAB has found utility in the ultrastructural localization of specific proteins that have been genetically labeled with peroxidase tags or fluorochromes, the latter being visualized by the process of photoconversion which exploits the fact that fluorescence excitation can be coupled to the polymerization of DAB. Finally, the future of DAB in the life sciences, whether in its native form or some modified version, is speculated on.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"254-273"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined melatonin and curcumin treatment ameliorates renal ischemia-reperfusion injury via reducing oxidative stress, inflammation, and apoptosis in rats.","authors":"S Topkaraoglu, T Sapmaz, K Sevgin, M Tekayev, S Kuras, H H Pence, E M Guler, S Aktas, O Irkorucu","doi":"10.1080/10520295.2026.2642383","DOIUrl":"10.1080/10520295.2026.2642383","url":null,"abstract":"<p><p>Renal ischemia-reperfusion (Isc-Rep) injury commonly occurs during kidney transplantation, renal vascular surgery, or traumatic hemorrhagic shock, and is marked by excessive oxidative stress and inflammation. We aimed to examine the antioxidant properties of the melatonin-curcumin combination in an experimental renal Isc-Rep model in rats. Thirty-five rats were allocated into five distinct experimental groups. While the rats in the Control (C) group did not undergo any surgical procedure, each of the rats in the remaining groups underwent 45 minutes of renal ischemia followed by 2 h of reperfusion. In all experimental groups, except the Isc-Rep group, the rats were administered an active compound immediately prior to reperfusion, namely melatonin (MEL, 20 mg/kg), curcumin (CUR, 200 mg/kg), or a combination of MEL-CUR. In all groups, histological evaluation was carried out, while apoptotic cell analyses were similarly performed by means of the TUNEL method. In addition, oxidative stress parameters (TOS, TAS, OSI), inflammatory cytokines (TNF-α, IL-6, and IL-1β), superoxide-dismutase (SOD), and malondialdehyde (MDA) activity levels were also examined. Histological evaluation demonstrated that renal Isc-Rep-induced cellular damage, tubular dilatation, and inflammation were significantly reduced in the MEL-CUR groups in comparison with the Isc-Rep group (p < 0.005). Furthermore, statistically significant differences were observed among the groups in TOS, TAS, OSI, TNF-α, IL-6, and IL-1β levels (p < 0.005). The co-administration of melatonin and curcumin resulted in greater protection against ischemia-reperfusion-induced renal damage compared with either treatment alone. Moreover, the co-administration of these antioxidant agents demonstrates greater therapeutic efficacy compared to their individua.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"215-225"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neuroprotective effects of thalidomide in cerebral ischemia-reperfusion injury induced by bilateral carotid artery occlusion in rats.","authors":"Onural Ozhan, Ugur Cem Mete, Azibe Yildiz, Merve Durhan, Nigar Vardi, Yilmaz Cigremis, Gul Busra Kaya, Ahmet Acet, Hakan Parlakpinar","doi":"10.1080/10520295.2026.2648550","DOIUrl":"10.1080/10520295.2026.2648550","url":null,"abstract":"<p><p>This study investigates the neuroprotective effects of thalidomide (THA) in cerebral ischemia-reperfusion (I/R) injury induced by bilateral carotid artery occlusion in rats. Thirty-two female Wistar albino rats were divided into four groups: Sham, I/R, THA+I/R, and I/R+THA. I/R injury was induced by occluding both carotid arteries for 15 minutes followed by 24-hour reperfusion. THA (20 mg/kg) was administered intraperitoneally either before or after ischemia. Biochemical analyses measured malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in brain tissues. Histopathological evaluations assessed neuronal degeneration and tissue damage. The I/R group exhibited significantly increased MDA levels and reduced SOD and GSH enzyme activities in cerebrum and cerebellum tissues (P < 0.05). THA treatment significantly decreased MDA levels and restored SOD and GSH enzyme activities in both THA+I/R and I/R+THA groups (P < 0.05). Histopathological analyses revealed neuronal degeneration in the I/R group, whereas THA treatment mitigated neuronal damage, particularly in the I/R+THA group. In the IR+THA group, there was a significant decrease in the severity of caspase-3 immunoreactivity compared to the I/R group. THA demonstrates neuroprotective effects against cerebral I/R injury by reducing oxidative stress and neuronal damage. Administration of THA post-ischemia appears to be more effective in preserving neuronal integrity. These findings suggest that THA has neuroprotective potential in an experimental model of cerebral I/R injury; however, given its known adverse effects, further studies focusing on safety, dosing, and risk-benefit balance are required before any clinical implications can be considered.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"240-253"},"PeriodicalIF":1.4,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postradiation angiosarcoma of the breast: a 25-year single-institution analysis of clinicopathological characteristics and immunohistochemical biomarker study.","authors":"Kristin J Rybski, Brian S Finkelman, Bin Zhang, Ajay Dhakal, Bradley M Turner, David G Hicks, Huina Zhang","doi":"10.1080/10520295.2026.2626268","DOIUrl":"10.1080/10520295.2026.2626268","url":null,"abstract":"<p><p>We examined the clinicopathologic features and immunohistochemical expression of commonly tested biomarkers relevant to targeted therapy in postradiation angiosarcoma (PRAS) of the breast, aiming to better understand tumor biology and provide baseline data on tumor biomarkers to inform future therapeutic strategies for PRAS. Clinicopathologic features, outcomes, and the immunohistochemical expression of tumor biomarkers, including human epidermal growth factor receptor 2 (HER2), programmed death-ligand 1 (PD-L1), and DNA mismatch repair proteins (MMR), were analyzed in breast PRAS specimens diagnosed from 2000 to 2024. A total of 24 breast PRAS specimens from 18 patients were included in the study. All PRAS patients were female, with a median age of 72 years at the time of diagnosis. High-grade tumors were observed in 47%, 43%, and 65% of specimens according to Fédération Nationale des Centers de Lutte Contre le Cancer (FNCLCC), Donnell-Rosen, and Kuba scoring methods, respectively. Surgical intervention, adjuvant chemotherapy, and radiotherapy were administered to 100%, 29%, and 23% of patients, respectively. Five patients (29%) died from the disease during a median follow-up period of 37 months. Exploratory analysis showed there was no significant association between histologic grade and overall survival, regardless of the grading system used. All PRASs were MMR-proficient with a low number of tumor-infiltrating lymphocytes (TILs) and exhibited an absence of HER2 protein expression. PD-L1 was positive in seven (32%) specimens and positively correlated with stromal TILs. Our findings suggest that breast PRAS are genomically stable and poorly immunogenic, and are likely ineligible for any HER2-targeted therapies based on current approval criteria. A subset of specimens demonstrated PD-L1 positivity, underscoring the need for further investigation of immunotherapy with anti-PD1/PD-L1 immune checkpoint inhibitors as a potential treatment option.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"173-183"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146200080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hsa-miR-10a-5p/RNF186 modulates endoplasmic reticulum stress to exacerbate the development of ulcerative colitis.","authors":"Yifan Guo, Haidong Wu, Xuyong Chen, Fan Jiang, Ye Zhao, Xinpu Miao","doi":"10.1080/10520295.2025.2606089","DOIUrl":"10.1080/10520295.2025.2606089","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a chronic inflammatory bowel disease, and endoplasmic reticulum stress (ERS) may contribute to the pathogenesis and progression of UC. Previous research has found that hsa-miR-10a-5p is abnormally expressed in UC, but its molecular mechanisms remain unclear. This study aimed to explore the role of hsa-miR-10a-5p in regulating ERS in UC through RNF186. LPS-induced HT-29 cells and a dextran sulfate sodium (DSS)-induced animal model were utilized to explore the regulatory roles of hsa-miR-10a-5p and RNF186 in ERS in UC. Following modulation of hsa-miR-10a-5p and RNF186 expression, we assessed the expression of ERS-related genes in the UC model using qPCR and immunofluorescence, and evaluated apoptosis with flow cytometry and WB. Furthermore, we conducted DAI scoring, HE staining, and permeability testing in the animal model, and analyzed the inflammatory profile of UC by ELISA to further understand disease progression and the impact of molecular changes on intestinal pathology. Overexpression of hsa-miR-10a-5p in HT-29 cells and animal models promoted cell proliferation, inhibited apoptosis, and mitigated inflammatory factor release and ERS response. Conversely, miR-10a-5p knockdown activated colonic mucosal epithelial damage, reduced cell proliferation, increased apoptosis, aggravated ERS response, and enhanced inflammatory factor expression. This research elucidated that hsa-miR-10a-5p participates in the process of UC development and progression by modulating the ERS response through RNF186. This discovery offered novel insights, enhancing our comprehension of the underlying pathophysiology of UC and provided a theoretical basis for the potential application of miRNAs in UC therapy.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"145-160"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145932045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical labeling of carbonic anhydrase isoenzymes in labial glands of infants.","authors":"Mechthild Stoeckelhuber, Klaus-Dietrich Wolff, Marco R Kesting, Denys J Loeffelbein, Christoph Schmitz, Lucas M Ritschl","doi":"10.1080/10520295.2026.2618820","DOIUrl":"10.1080/10520295.2026.2618820","url":null,"abstract":"<p><p>In salivary glands, the concurrent availability of HCO3^- is required for normal mucus release that is guaranteed by the carbonic anhydrases in this area. Due to lack of detailed carbonic anhydrase data in minor salivary glands, we identified various carbonic anhydrases (CA) in the human labial glands of infants in this study. Specifically, the CA isoenzymes II, III, IV, VI, VII, and XII were investigated in secretory and ductal epithelial cells. CA II was detected in serous glandular cells and sporadically in ductal cells, CA III in myoepithelial cells and skeletal muscle cells, CA IV in ductal cells, endothelial cells, erythrocytes, and skeletal muscle. The first immunohistochemical analysis of CA VII in salivary glands resulted in a positive reaction in serous glandular cells and ductal cells, as well as in skeletal muscle and endothelial cells. CA XII was sporadically localized at the basolateral membrane of ductal cells and in serous glandular cells. Mucous endpieces were negative in all carbonic anhydrases tested. Knowledge of carbonic anhydrases distribution in healthy tissues supports their assessment as biomarkers for cancer diagnosis, prevention, and therapy.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"207-213"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}