Biotechnic & Histochemistry最新文献_第4页

Diazoxide attenuates DOX-induced cardiotoxicity in cultured rat myocytes. 重氮氧化物可减轻 DOX 诱导的大鼠心肌细胞毒性。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-05 DOI: 10.1080/10520295.2024.2324368

Celal Guven, Eylem Taskin, Özgül Aydın, Salih Tunç Kaya, Yusuf Sevgiler

{"title":"Diazoxide attenuates DOX-induced cardiotoxicity in cultured rat myocytes.","authors":"Celal Guven, Eylem Taskin, Özgül Aydın, Salih Tunç Kaya, Yusuf Sevgiler","doi":"10.1080/10520295.2024.2324368","DOIUrl":"10.1080/10520295.2024.2324368","url":null,"abstract":"Doxorubicin (DOX)-induced cardiotoxicity is a well known clinical problem, and many investigations have been made of its possible amelioration. We have investigated whether diazoxide (DIA), an agonist at mitochondrial ATP-sensitive potassium channels (mitoKATP), could reverse DOX-induced apoptotic myocardial cell loss, in cultured rat cardiomyocytes. The role of certain proteins in this pathway was also studied. The rat cardiomyocyte cell line (H9c2) was treated with DOX, and also co-treated with DOX and DIA, for 24 h. Distribution of actin filaments, mitochondrial membrane potential, superoxide dismutase (SOD) activity, total oxidant and antioxidant status (TOS and TAS, respectively), and some protein expressions, were assessed. DOX significantly decreased SOD activity, increased ERK1/2 protein levels, and depolarised the mitochondrial membrane, while DIA co-treatment inhibited such changes. DIA co-treatment ameliorated DOX-induced cytoskeletal changes via F-actin distribution and mitoKATP structure. Co-treatment also decreased ERK1/2 and cytochrome c protein levels. Cardiomyocyte loss due to oxidative stress-mediated apoptosis is a key event in DOX-induced cytotoxicity. DIA had protective effects on DOX-induced cardiotoxicity, via mitoKATP integrity, especially with elevated SUR2A levels; but also by a cascade including SOD/AMPK/ERK1/2. Therefore, DIA may be considered a candidate agent for protecting cardiomyocytes against DOX chemotherapy.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"113-124"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140027230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tribute to G. Stephen Nettleton (May 7, 1946-April 2, 2024). 向斯蒂芬-内特尔顿（G. Stephen Nettleton，1946 年 5 月 7 日-2024 年 4 月 2 日）致敬。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-05-13 DOI: 10.1080/10520295.2024.2347029

Richard W Dapson

引用次数: 0

Effect of leptin on nitrergic neurons in the lateral hypothalamic area and the supraoptic nucleus of rats. 瘦素对大鼠下丘脑外侧区和视上核硝酸神经元的影响

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-27 DOI: 10.1080/10520295.2024.2335167

Milen Hristov, Boycho Landzhov, Krassimira Yakimova

引用次数: 0

Laminin receptor 1 expression in premalignant and malignant squamous lesions of the cervix. 宫颈癌前病变和恶性鳞状病变中层粘蛋白受体 1 的表达。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-05-13 DOI: 10.1080/10520295.2024.2346912

Mertihan Kurdoğlu, Zehra Kurdoğlu, Zehra Küçükaydın, Remzi Erten, Gülay Bulut, Süleyman Özen

{"title":"Laminin receptor 1 expression in premalignant and malignant squamous lesions of the cervix.","authors":"Mertihan Kurdoğlu, Zehra Kurdoğlu, Zehra Küçükaydın, Remzi Erten, Gülay Bulut, Süleyman Özen","doi":"10.1080/10520295.2024.2346912","DOIUrl":"10.1080/10520295.2024.2346912","url":null,"abstract":"Laminin receptor 1 (LAMR) may have a role in the progression of premalignant squamous epithelial lesions to cervical cancer. Therefore, we aimed to investigate the expression of laminin receptor 1 (LAMR) in normal, premalignant, and malignant tissues of the uterine cervix. Paraffin blocks of 129 specimens with the diagnoses of normal cervical tissue (n = 33), cervical intraepithelial neoplasia (CIN) 1 (n = 30), CIN 2 (n = 14), CIN 3 (n = 28), and squamous cell carcinoma (n = 24) were immunohistochemically stained with LAMR antibody and its expression percentage, pattern, and intensity in these tissues were assessed. Compared to the other groups, the nonstaining with LAMR was highest in low grade squamous intraepithelial lesion (LSIL) (p < 0.0001). LAMR expression, which was positive in less than 50% of cells with weak staining, increased significantly between normal cervical epithelium and high-grade squamous intraepithelial lesion (HSIL) or invasive carcinoma, as well as between LSIL and HSIL (p < 0.0001). Between LSIL and invasive carcinoma, a significant increment was also observed for weak staining in less than 50% of cells (p < 0.001). LAMR expression, which was positive in more than 50% of cells with strong staining, was significantly higher in normal cervical tissue compared to the other groups (p < 0.0001). Disease progression related gradual increment of LAMR expression from normal cervical epithelium or LSIL towards HSIL or cervical cancer reveals that LAMR may play an important role in the transition from premalignant to malignant state in cervical lesions.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"99 3","pages":"174-181"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A histological examination of the effects of Ferula elaeochytris extract on kidney and liver tissues in myoglobinuric acute renal failure. 阿魏提取物对肌红蛋白尿急性肾衰竭患者肾脏和肝脏组织影响的组织学研究。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-14 DOI: 10.1080/10520295.2024.2323973

Fatma Yıldız, Meltem Güngör, Perihan Sezginer, Tiince Aksak

{"title":"A histological examination of the effects of Ferula elaeochytris extract on kidney and liver tissues in myoglobinuric acute renal failure.","authors":"Fatma Yıldız, Meltem Güngör, Perihan Sezginer, Tiince Aksak","doi":"10.1080/10520295.2024.2323973","DOIUrl":"10.1080/10520295.2024.2323973","url":null,"abstract":"Myoglobinuric acute renal failure (MARF) is a structural and functional disorder that occurs in the kidney following the release of muscle cell contents into the circulation. In this present study, possible protective and curative effects of Ferula elaeochytris extract against kidney and liver damage in experimentally induced MARF in a rat model were investigated. 3-4 Month-old, 200-250 g Sprague Dawley rats were divided into 8 equal groups with 7 rats per group. Group I was a no-intervention Control group. All groups except for the Group I were dehydrated for 16 hours. Following this dehydration, 50% v/v aqueous glycerol solution was injected into both hind leg muscles of the animals, at a dose of 8 ml/kg. The rats were given physiological saline (SF) once orally before the model was administered (Group II) and after the model was administered (Group V). Similarly, two different doses of Ferula elaeochytris root extract (40 mg/kg and 80 mg/kg) were dissolved in 2 ml of SF and administered orally before (Groups III and IV) and after (Group VI, VII) the model was created. Following the experimental period, kidney and liver tissues were removed from all groups, and fixed in 10% neutral formaldehyde solution for light microscopic examinations. Intracellular vacuolization, enlargement in the Bowman's space, widespread atrophy in the tubular structures, luminal enlargement, and desquamation were detected in the kidney tissue sections of all the experimental model groups. In the liver tissue sections, was detected hepatocyte degeneration, intracellular vacuolization, irregularity in cell membrane borders, and apoptotic bodies. These histopathological consequences of MARF were evaluated for all groups, and whereas a curative effect of Ferula elaeochytris could be seen, its protective effect was higher than its curative effect.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"103-112"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140118691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nobiletin alleviates methotrexate-induced hepatorenal toxicity in rats. Nobiletin 可减轻甲氨蝶呤引起的大鼠肝肾毒性。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-04-02 DOI: 10.1080/10520295.2024.2335168

Filiz Kazak, Ahmet Uyar, Pinar Coskun, Turan Yaman

{"title":"Nobiletin alleviates methotrexate-induced hepatorenal toxicity in rats.","authors":"Filiz Kazak, Ahmet Uyar, Pinar Coskun, Turan Yaman","doi":"10.1080/10520295.2024.2335168","DOIUrl":"10.1080/10520295.2024.2335168","url":null,"abstract":"We investigated the possible ameliorative effects of nobiletin (NBL) against methotrexate (MTX)-induced hepatorenal toxicity in rats. Twenty-eight Wistar albino rats were randomly divided into four groups, namely: Control; MTX (administered 20 mg/kg MTX); MTX+NBL (administered 20 mg/kg MTX and 10 mg/kg NBL per day); and NBL (administered 10 mg/kg/day NBL). Histopathological, immunohistochemical and biochemical analyses were performed on the kidney and liver tissues of rats at the end of the study. MTX caused renal toxicity, as indicated by increases in malondialdehyde (MDA) and caspase-3, as well as decreases in reduced glutathione (GSH), glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx), catalase (CAT) and B-cell lymphoma-2 (Bcl-2). MTX also caused hepatotoxicity, as indicated by increases in 8-hydroxy-2'-deoxyguanosine (8-OHdG), tumor necrosis factor alpha (TNF-α), MDA and caspase-3 and decrease in interleukin 10 (IL-10), GSH, total antioxidant capacity, GPx, G6PD, CAT and Bcl-2. MTX caused histopathological changes in kidney and liver tissues indicating tissue and cellular damage. Administration of NBL concurrently with methotrexate reduced oxidative stress, inflammatory and apoptotic signs, and prevented kidney and liver damage caused by methotrexate. We consider NBL has attenuating and ameliorating effects on methotrexate-induced hepatorenal toxicity.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"134-146"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of Myrtus communis L. extract involving the inhibition of oxidative stress on high fat diet-induced testis damage in rats. 香桃木提取物对高脂饮食诱发的大鼠睾丸损伤具有抑制氧化应激的改善作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-04-29 DOI: 10.1080/10520295.2024.2344491

Busra Coskunlu, M Kutay Koroglu, Irem Hersek, Busra Ertas, Ali Sen, Goksel Sener, Feriha Ercan

{"title":"Ameliorative effects of Myrtus communis L. extract involving the inhibition of oxidative stress on high fat diet-induced testis damage in rats.","authors":"Busra Coskunlu, M Kutay Koroglu, Irem Hersek, Busra Ertas, Ali Sen, Goksel Sener, Feriha Ercan","doi":"10.1080/10520295.2024.2344491","DOIUrl":"10.1080/10520295.2024.2344491","url":null,"abstract":"The possible protective effects of Myrtus communis L. (MC) extract on a high fat diet (HFD)-induced testicular injury in a rat model were investigated using histological and biochemical methods. Wistar albino rats were divided into three groups: a standard diet control group; a HFD group; and an HFD+MC group. The HFD and HFD+MC groups were fed with a HFD for 16 weeks. MC extract (100 mg/kg) was given orally five days a week to the rats in the HFD+MC group during the last four weeks of the experiment. Leptin, triglyceride, high-density lipoproteins, cholesterol, estrogen, testosterone, LH and FSH were analyzed in blood serum. Sperm parameters were evaluated from the epididymis. Testicular morphology, proliferative, apoptotic and NADPH oxidase-2 (NOX2)-positive cells were evaluated histologically. Testicular oxidative stress parameters were analyzed biochemically. In the HFD group, lipid and hormone profiles were changed, abnormal spermatozoa, degenerated seminiferous tubules with apoptotic and NOX2-positive cells were increased in number, and sperm motility and germinal proliferative cells decreased compared to the control group. Moreover, testicular malondialdehyde, 8-hydroxy-2-deoxyguanosine and myeloperoxidase levels increased, whereas glutathione and superoxide dismutase levels decreased in the HFD group compared to the control group. All these histological and biochemical features were ameliorated by MC treatment of HFD-fed rats. In conclusion, HFD caused alterations in sperm parameters and testicular morphology by increasing oxidative damage and apoptosis. MC extract may have potential protective effects by inhibiting oxidative damage.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"157-173"},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140861838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-04-05 DOI: 10.1080/10520295.2024.2335836

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Stains recently certified. 污渍最近通过了认证。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-03-22 DOI: 10.1080/10520295.2024.2331320

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Reviewers for Biotechnic & Histochemistry, 2023 2023 年生物技术与组织化学》审稿人

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-03-06 DOI: 10.1080/10520295.2024.2303898

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