Biotechnic & Histochemistry最新文献

{"title":"Assessment of the protective effects of tannic acid against bleomycin-induced lung fibrosis in rats.","authors":"Amir Siahpoosh, Narges Atefipour, Mohammadreza Momeni Qomi, Mehdi Goudarzi, Laya Sadat Khorsandi, Rezvan Hafezi, Ali Reza Malayeri","doi":"10.1080/10520295.2025.2611824","DOIUrl":"10.1080/10520295.2025.2611824","url":null,"abstract":"<p><p>Pulmonary fibrosis (PF) is a prevalent adverse effect associated with bleomycin (Bleo) administration. Despite extensive research efforts, there is no effective treatment currently available for PF. The current study investigates the antifibrotic and antioxidant properties of tannic acid (TA), a natural polyphenol known for its potent antioxidant and anti-inflammatory activities, in the context of Bleo-induced PF in rats. Fifty Wistar rats were randomly assigned to five distinct groups for the study. Group 1 served as the control (normal saline). Group 2 received a single dose of Bleo (7.5 U/kg via intratracheal injection) on the 7th day. Groups 3, 4, and 5 were treated with 100, 200, and 400 mg/kg of TA, respectively, administered 7 days prior to and 21 days following Bleo administration. The rats were euthanized 24 hours after the last administration. The protective effects of TA were assessed through histopathological and biochemical analyses, which included measurements of lung index, hydroxyproline (HP), nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). Additionally, the influence of TA on tumor necrosis factor-α (TNF-α) levels was evaluated. The results indicated that TA significantly reduced the Bleo-induced increase in lung index, MDA, NO, HP, and TNF-α levels. Furthermore, TA enhanced the activities of CAT, SOD, and GPx, as well as the content of GSH. Additionally, TA mitigated the infiltration of fibroblasts and inflammatory cells and prevented alveolar thickening induced by Bleo. The findings demonstrated that TA exhibits a protective effect against PF induced by Bleo.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"161-172"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146177672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and evaluation of an in-situ hybridization assay for infectious pancreatic necrosis virus.","authors":"Nihat Toplu, Kubilay Metin, Harun Albayrak, Tuba Çiğdem Oğuzoğlu, Erkemen Tuğrul Epikmen, Emrah İpek, Ayşe Nur Akkoç","doi":"10.1080/10520295.2026.2640836","DOIUrl":"10.1080/10520295.2026.2640836","url":null,"abstract":"<p><p>In the present study, an in-situ hybridization (ISH) assay was performed on cell lines and paraffin-embedded tissue blocks infected with infectious pancreatic necrosis (IPN) virus in fish. The molecular diagnosis by ISH was established through four main steps: (1) generation of DIG (Digoxigenin)-labeled probes, (2) production of anti-DIG antibodies and their conjugation with alkaline phosphatase, (3) preparation of ISH reagents, including pre-hybridization and hybridization solutions, and (4) application of the ISH assay to cell culture and paraffin tissue sections. Protease concentrations, hybridization solutions, and assay modifications were optimized separately for cell lines and paraffin sections. Ultimately, ISH assays using DIG-labeled probes targeting the VP2 and VP3 gene regions of IPN virus were successfully performed in virus-infected cell lines and paraffin-embedded tissues.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"184-194"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the expressions of GPR30, GPR120 and GPR125 proteins in rat testis in the experimentally created Parkinson model.","authors":"S Ünver Saraydin, M Ergin","doi":"10.1080/10520295.2026.2641746","DOIUrl":"10.1080/10520295.2026.2641746","url":null,"abstract":"<p><p>Parkinson's disease (PD) is a progressive neurodegenerative disorder associated with aging. It is characterized by both motor and non-motor symptoms, including cognitive impairment, mood disturbances, sleep disruption, sexual dysfunction, and weight loss. These symptoms may precede a clinical diagnosis. Sex-related differences in PD, influenced by estrogen's neuroprotective effects and testosterone deficiency, contribute to a higher prevalence and severity of the disease in males. Male rats received daily subcutaneous injections of rotenone (2 mg/kg) for 20 days to establish a PD model, which enabled the investigation of central dopaminergic degeneration and peripheral testicular effects. Tyrosine hydroxylase (TH) immunoreactivity in the substantia nigra pars compacta (SNpc) was assessed alongside the testicular expression of GPR30, GPR120, and GPR125 in the testes using immunohistochemical and immunofluorescence techniques. Rotenone-treated animals exhibited significant weight loss (z = -4.292, <i>p</i> < 0.001), reduced TH expression in the SNpc, and hallmark PD pathology including Lewy body-like inclusions, Lewy neurites, and halo-like structures around dopaminergic neuron nuclei. Testicular analysis revealed a time-dependent decline in GPR protein expression. GPR30 was found in spermatids and interstitial cells, GPR120 was found predominantly in Leydig cells, and GPR125 was found in interstitial tissue, Sertoli cells, and spermatocytes. These findings suggest that the loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) may affect testicular function via testosterone-dependent regulation of GPR30, GPR120, and GPR125 expression. This highlights the systemic nature of PD and its potential impact on male reproductive health. Alterations in testicular G Protein-Coupled Receptors (GPCRs) may serve as peripheral biomarkers of disease progression, emphasizing the importance of considering endocrine and reproductive dysfunction in both experimental models and the clinical management of PD.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"195-206"},"PeriodicalIF":1.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling mechanistic effects of mast cell in the progression of fibrosis and malignant transformation of oral submucous fibrosis: a systematic review and meta-analysis.","authors":"Keerthika R, Akhilesh Chandra, Dinesh Raja, Rahul Agarwal","doi":"10.1080/10520295.2025.2595966","DOIUrl":"10.1080/10520295.2025.2595966","url":null,"abstract":"<p><p>Oral submucous fibrosis (OSMF) is a ubiquitous fatal fibrotic mucosal disease with multifactorial etiology and complex pathogenesis. The role of mast cells in the pathophysiology of OSMF remains uncharted territory owing to a dearth of studies. Thus, the present systematic review and meta-analysis aimed to unentangle the mysteric role of mast cells in the pathogenesis, progression of fibrosis and malignant transformation of OSMF. Using various databases, full-text articles that investigated mast cell concentrations in OSMF were entailed for review. A modified Newcastle-Ottawa scale was employed to evaluate the risk of bias in all articles and Review Manager was utilized for meta-analysis. Twenty and fourteen qualified articles, respectively, were included for qualitative and quantitative data synthesis. Progressive amplification of mast cell density is linked with fibrosis-induced malignant transformation of OSMF. The fixed-effect model also confirmed that significantly upregulated mast cell counts have a decreased risk of association with control as well as a significantly increased risk of being associated with early-stage fibrosis and malignant transformation of OSMF. This review authenticates the mechanistic effects of mast cells in the pathogenesis, chronicity, progression of fibrosis and malignant transformation of OSMF.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"109-118"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic and ultrastructural insights into the nephro-therapeutic role of chitosan nanoparticles against streptozotocin-induced diabetic nephropathy.","authors":"Aml A Elngar, Eman A Moussa, Felwa A Thagfan, Huda A Alqahtani, Reem Dkhil, Nabila M Mira, Shaimaa M Kasem, Mohamed A Dkhil, Nora F Ghanem","doi":"10.1080/10520295.2025.2603933","DOIUrl":"10.1080/10520295.2025.2603933","url":null,"abstract":"<p><p>Diabetes mellitus is a prevalent chronic disease, with diabetic nephropathy being a significant complication that causes structural alterations in the kidneys. The purpose of this research was to examine the effects of nano-chitosan (NCh) on the kidneys of rats with diabetes induced by streptozotocin (STZ). NCh was prepared and characterized using scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy technique (EDX) and zeta potential. A total of 40 male Sprague Dawley albino rats (225 ± 25 grams, 2.5-3 months old) were involved and divided into 4 equal groups, each with 10 rats: Control (Ctrl), non-diabetic-administered with 0.5 mg/kg B.W NCh (NCh), diabetic induced by STZ intraperitoneally (Diabetic), and diabetic treated with NCh at 0.5 mg/kg B.W (Diabetic-NCh). Seven days post-diabetic induction, NCh was inoculated orally for 21 days once a day. Body weight change and kidney function tests (creatinine and urea), as well as renal histopathological, histochemical (collagen fibers), immunohistochemical; alpha smooth muscle actin (αSMA) and transforming growth factor-beta1 (TGF-β1), and ultrastructural studies were involved. The findings indicated that diabetic-NCh treated rats exhibited improvements in body weight and kidney function tests including creatinine and urea, compared to the diabetic rats. NCh treatment enhanced renal tissue architecture and reduced collagen fiber expression. Immunohistochemical analysis showed decreased concentrations of αSMA and TGF-β1 in the Diabetic-NCh group. Ultrastructural studies confirmed the improvement in kidney tissue structure. In conclusion, oral administration of nano-chitosan demonstrated a potential therapeutic effect on kidneys in diabetic rats, suggesting its promise as a treatment strategy for diabetic nephropathy.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"119-132"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiapoptotic effects of anthocyanin on testicular damage induced by varicocele in male rats.","authors":"Ghasem Rostami, Mohammad Reza Navabakhsh, Seyedeh Sara Salami, Mohammad Rezaei, Elham Hasannezhad, Alireza Rahimi Mamaghani, Majid Shokoohi, Linda Mohammadzadeh Boukani","doi":"10.1080/10520295.2025.2595962","DOIUrl":"10.1080/10520295.2025.2595962","url":null,"abstract":"<p><p>Varicocele is one of the most important disorders causing infertility in men, and oxidative stress is one of the most important factors affecting testicular parenchyma damage caused by varicocele. This study explored the effect of anthocyanins on varicocele-induced testis injury in adult Wistar rats by focusing on regulating oxidative stress, Bax and Bcl-2 genes, and protein related to cell death. Rats (n = 32) were divided into four groups: Control (Sham), varicocele, varicocele + anthocyanin, and anthocyanin alone. At the end of the study (week 8), the animals were sacrificed, and H&E staining was used for testicular histopathology. The IHC method was used for the detection of Bax and Bcl-2 protein expression, and TUNEL assays were used to analyze testicular Apoptosis. Additionally, serum levels of oxidative stress markers - MDA, SOD, and GPx - were assessed by ELISA, and RT-qPCR analyzed the mRNA expression of Bax and Bcl-2. Histological analysis revealed notable improvements in Johnsen's score, epithelial thickness, and seminiferous tubule diameter in the varicocele + anthocyanin group relative to the varicocele-only group (<i>p </i>< 0.005). Protein and mRNA expression of Bax significantly increased in the varicocele group (<i>p </i>< 0.005), while treatment with anthocyanin enhanced Bcl-2 expression (<i>p </i>< 0.005). Furthermore, the rate of apoptotic positive germ cells decreased when the rats received anthocyanin. Moreover, anthocyanin increased serum levels of GPx and SOD while decreasing MDA levels in the treatment group compared to rats with varicocele (<i>p </i>< 0.005). These outcomes suggest that anthocyanin may moderate testicular injury from varicocele, primarily through its antioxidative properties.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"84-95"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CTRPs, β3-AR signaling, and placental fibrin deposition: molecular and histopathological aspects of preterm birth.","authors":"D Aşkin Özek, R Melekoğlu, N Akpolat, H Yüce, N Zeyveli-Çelik, Ş Yaşar, Y Berberoğlu, K Tanbek, M Hüz, I Ateş, S Ünüvar, S Sandal","doi":"10.1080/10520295.2025.2595963","DOIUrl":"10.1080/10520295.2025.2595963","url":null,"abstract":"<p><p>It has been suggested that adipokines may modulate plasma lipid levels, and β3-adrenergic receptor (β3-AR) gene expressions may affect adipokine levels and play critical roles in lipid metabolism. This study aims to determine predictive biomarkers for preterm birth (PTB) by evaluating serum complement 1q (C1q)/tumor necrosis factor (TNF)-related protein (CTRP) levels, lipid profiles, gene expressions, and placental pathological changes in women experiencing PTB. A total of 80 pregnant women, 40 preterm and 40 term, who applied to the Department of Obstetrics and Gynecology, Faculty of Medicine, Inonu University, were included in the study. Blood and placenta samples were taken from all participants. Serum CTRP, high-density lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol (TC), and triglyceride (TG) levels were measured; β3-AR gene expression and detection rate of the β3-AR <i>rs4994</i> (Trp64Arg) amplicon were evaluated. Placental tissues were examined histopathologically for perivillous/intervillous fibrin deposition and hydropic degeneration. ROC analysis was used to determine predictive biomarkers for PTB. In the PTB group, compared to the control group, β3-AR gene expression levels and serum CTRP3 levels were significantly decreased, while the detection rate of the Trp64Arg amplicon and serum CTRP4 levels were significantly increased. In addition, LDL levels increased significantly (<i>p</i> = 0.046), TC levels decreased (<i>p</i> = 0.045). According to ROC analysis, LDL (<i>p</i> = 0.039), TC (<i>p</i> = 0.034), CTRP3 (<i>p</i> = 0.019), and CTRP4 (<i>p</i> = 0.033) levels were determined as significant predictive biomarkers for PTB. Histopathological examination revealed increased perivillous and intervillous fibrin deposition and marked hydropic degeneration in the PTB group. Changes in CTRP levels, lipid profile disorders, and a decrease in β3-AR signaling pathways were found to be associated with PTB. LDL, TC, CTRP3, and CTRP4 levels can be evaluated as potential biomarkers that can be used in the early diagnosis and management of PTB.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"96-108"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145767177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of tanshinone IIA in folic acid-induced acute kidney injury: a network pharmacology approach.","authors":"Peijian Chen, Ping He, Jieting Liu, Ran Guo, Yanhui Chu, Yang Xiao, Minglu Ding","doi":"10.1080/10520295.2025.2589264","DOIUrl":"10.1080/10520295.2025.2589264","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a clinical syndrome that can be caused by a variety of factors, leading to rapid decline of kidney function and increased morbidity and mortality, whilst also exerting significant economic burden on the affected patient. <i>Salvia miltiorrhiza</i> is a highly valued plant in traditional Chinese medicine (TCM). Tanshinone IIA (Tan IIA) is an important active compound that can be extracted from salvia miltiorrhiza, which has reported anti-inflammatory effects. The objective of the present investigation was to explore the potential effects of Tan IIA on folic acid-induced AKI and elucidate its underlying mechanism. A comprehensive analysis was conducted utilizing the TCM Systematic Pharmacology Database and Analytical Platform database to screen for chemical components and their corresponding targets. Subsequently, by using network pharmacology techniques and Cytoscapes 3.7.2 software, a protein-protein interaction (PPI) network was constructed and analyzed. Through Venn diagram analysis of the DeGeNET, OMIM, PharmGKB, and GeneCards databases using the key word \"acute kidney injury,\" a total of 76 overlapping targets were obtained. Building upon this, a compound-target gene network was constructed and analyzed by Cytoscapes 3.7.2 software, revealing TP53, STAT3, CASP3, VEGFA, and JUN to be pivotal therapeutic targets. Subsequently, an AKI mouse model was established to investigate the renal effects of Tan IIA. By immunohistochemistry, Western blot results showed the Tan IIA ameliorated kidney function by alleviating inflammation, mitigating necrosis of renal tubular cells, promoting their proliferation and attenuating kidney injury. These beneficial effects were found to be achieved by inhibiting the PI3K/AKT signaling pathway and inhibiting the expression of TP53 by Western blot. In conclusion, TP53may be a potential target for folic acid-induced AKI, whilst Tan IIA exerts its renoprotective effects and improves renal function by PI3K/AKT signaling pathways.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"69-83"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145699726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of stromal vascular fraction cells derived from epididymal and inguinal adipose tissues on <i>in vitro</i> spermatogenesis.","authors":"Nilgün Öksel, Merve Bulut, Selin Önen, Merve Gizer, Zeliha Cansel Özmen, Petek Korkusuz","doi":"10.1080/10520295.2025.2604573","DOIUrl":"10.1080/10520295.2025.2604573","url":null,"abstract":"<p><p>Infertility affects 10-20% of sexually active couples and male-related factors contribute to 30-50% of all cases of infertility. The development of three-dimensional in vitro approaches to promote the differentiation of spermatogonial stem cells (SSCs) into functional spermatozoa is an essential step for the treatment of male infertility. Stromal vascular fraction (SVF) cells derived from adipose tissue are known to have a regenerative effect on spermatogenesis and testicular regeneration after testicular damage. The study assessed the effect of SVF cells from epididymal and inguinal adipose tissue on in vitro spermatogenesis. Testicular cells from prepubertal mice were cultured alone as control and co-cultured with SVF cells from epididymal (ESVF) and inguinal (ISVF) adipose tissue in experimental groups by an air-liquid interface system. Spermatogenic progression was evaluated histomorphometrically and immunohistochemically at weeks 1, 3, 4, and 6. ESVF increased formation of tubule-like structures at week 1 and ISVF had a similar effect at week 4. The ISVF group showed higher numbers of ID4(+) (Inhibitor of DNA Binding 4) SSCs than control at all time points. At weeks 3 and 4, the ISVF exhibited increased number of SCP3(+) (Synaptonemal Complex Protein 3) spermatocytes compared to the control group and the ESVF showed a similar increase at week 6. The presence of ACR(+) (Acrosin) spermatids was observed in all groups at week 3. At week 4, the ISVF group had more ACR(+) spermatids than the control and ESVF groups. Our findings demonstrated that SVF cells effectively supported in vitro spermatogenesis. Notably, inguinal-derived SVF cells led to a higher production of ACR(+) spermatids than edidiymal-derived SVF cells. In conclusion, inguinal derived SVF cells can be used as a new co-culture method to preserve the SSC pool and promote in vitro spermatogenesis in infertile patients.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"133-143"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of programmed death-ligand 1 protein in head and neck squamous cell carcinoma and its clinicopathological correlates.","authors":"Abdullah Ahmad Bader, Manal A Abbas, Maher A Sughayer","doi":"10.1080/10520295.2025.2583966","DOIUrl":"10.1080/10520295.2025.2583966","url":null,"abstract":"<p><p>The ability to escape immune surveillance is a hallmark of malignancy. Programmed death ligand 1 (PD-L1) facilitates tumor progression by binding to the immune inhibitory receptor known as programmed cell death protein 1 (PD1) on immune cells, resulting in suppression of the cytotoxic T lymphocyte function. The degree of PD-L1 expression may have a prognostic value in some cancer types, and it may vary according to the genetic makeup and the ethnicity of patients. The expression level of PD-L1 in 63 cases of primary head and neck squamous cell carcinoma (HNSCC) tumor tissues was evaluated using immunohistochemistry (IHC). Also, PD-L1 association with various clinicopathologic characteristics and overall survival was studied. The positive expression rate of PD-L1 in HNSCC was 85.7%, 60.3%, and 52.3% of the total number of cases using combined positive score (CPS)<i> ≥ </i>1, CPS<i> ≥ </i>5, and CPS <i>≥ </i>20 cutoff values, respectively. Statistical analysis revealed no significant relationship between the expression of PD-L1 protein and clinicopathological features except for tobacco use using a cutoff CPS ≥ 20. The log-rank chi-square results showed that PD-L1 was not a significant factor affecting the 4-year overall survival of HNSCC patients. Also, the overall survival rate was not significantly affected by the patient's age, tumor differentiation, tumor size, and lymphovascular invasion. However, survival curves demonstrated lower overall survival in HNSCC female patients, disease recurrence, and positive perineural invasion. Our findings showed relatively high PDL-1 expression in most HNSCC patients. No significant association was found between PD-L1 protein expression and overall survival.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"21-29"},"PeriodicalIF":1.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145647260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}