Biotechnic & Histochemistry最新文献_第4页

Effect of leptin on nitrergic neurons in the lateral hypothalamic area and the supraoptic nucleus of rats. 瘦素对大鼠下丘脑外侧区和视上核硝酸神经元的影响

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-27 DOI: 10.1080/10520295.2024.2335167

Milen Hristov, Boycho Landzhov, Krassimira Yakimova

引用次数: 0

Laminin receptor 1 expression in premalignant and malignant squamous lesions of the cervix. 宫颈癌前病变和恶性鳞状病变中层粘蛋白受体 1 的表达。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-05-13 DOI: 10.1080/10520295.2024.2346912

Mertihan Kurdoğlu, Zehra Kurdoğlu, Zehra Küçükaydın, Remzi Erten, Gülay Bulut, Süleyman Özen

{"title":"Laminin receptor 1 expression in premalignant and malignant squamous lesions of the cervix.","authors":"Mertihan Kurdoğlu, Zehra Kurdoğlu, Zehra Küçükaydın, Remzi Erten, Gülay Bulut, Süleyman Özen","doi":"10.1080/10520295.2024.2346912","DOIUrl":"10.1080/10520295.2024.2346912","url":null,"abstract":"Laminin receptor 1 (LAMR) may have a role in the progression of premalignant squamous epithelial lesions to cervical cancer. Therefore, we aimed to investigate the expression of laminin receptor 1 (LAMR) in normal, premalignant, and malignant tissues of the uterine cervix. Paraffin blocks of 129 specimens with the diagnoses of normal cervical tissue (n = 33), cervical intraepithelial neoplasia (CIN) 1 (n = 30), CIN 2 (n = 14), CIN 3 (n = 28), and squamous cell carcinoma (n = 24) were immunohistochemically stained with LAMR antibody and its expression percentage, pattern, and intensity in these tissues were assessed. Compared to the other groups, the nonstaining with LAMR was highest in low grade squamous intraepithelial lesion (LSIL) (p < 0.0001). LAMR expression, which was positive in less than 50% of cells with weak staining, increased significantly between normal cervical epithelium and high-grade squamous intraepithelial lesion (HSIL) or invasive carcinoma, as well as between LSIL and HSIL (p < 0.0001). Between LSIL and invasive carcinoma, a significant increment was also observed for weak staining in less than 50% of cells (p < 0.001). LAMR expression, which was positive in more than 50% of cells with strong staining, was significantly higher in normal cervical tissue compared to the other groups (p < 0.0001). Disease progression related gradual increment of LAMR expression from normal cervical epithelium or LSIL towards HSIL or cervical cancer reveals that LAMR may play an important role in the transition from premalignant to malignant state in cervical lesions.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A histological examination of the effects of Ferula elaeochytris extract on kidney and liver tissues in myoglobinuric acute renal failure. 阿魏提取物对肌红蛋白尿急性肾衰竭患者肾脏和肝脏组织影响的组织学研究。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-14 DOI: 10.1080/10520295.2024.2323973

Fatma Yıldız, Meltem Güngör, Perihan Sezginer, Tiince Aksak

{"title":"A histological examination of the effects of Ferula elaeochytris extract on kidney and liver tissues in myoglobinuric acute renal failure.","authors":"Fatma Yıldız, Meltem Güngör, Perihan Sezginer, Tiince Aksak","doi":"10.1080/10520295.2024.2323973","DOIUrl":"10.1080/10520295.2024.2323973","url":null,"abstract":"Myoglobinuric acute renal failure (MARF) is a structural and functional disorder that occurs in the kidney following the release of muscle cell contents into the circulation. In this present study, possible protective and curative effects of Ferula elaeochytris extract against kidney and liver damage in experimentally induced MARF in a rat model were investigated. 3-4 Month-old, 200-250 g Sprague Dawley rats were divided into 8 equal groups with 7 rats per group. Group I was a no-intervention Control group. All groups except for the Group I were dehydrated for 16 hours. Following this dehydration, 50% v/v aqueous glycerol solution was injected into both hind leg muscles of the animals, at a dose of 8 ml/kg. The rats were given physiological saline (SF) once orally before the model was administered (Group II) and after the model was administered (Group V). Similarly, two different doses of Ferula elaeochytris root extract (40 mg/kg and 80 mg/kg) were dissolved in 2 ml of SF and administered orally before (Groups III and IV) and after (Group VI, VII) the model was created. Following the experimental period, kidney and liver tissues were removed from all groups, and fixed in 10% neutral formaldehyde solution for light microscopic examinations. Intracellular vacuolization, enlargement in the Bowman's space, widespread atrophy in the tubular structures, luminal enlargement, and desquamation were detected in the kidney tissue sections of all the experimental model groups. In the liver tissue sections, was detected hepatocyte degeneration, intracellular vacuolization, irregularity in cell membrane borders, and apoptotic bodies. These histopathological consequences of MARF were evaluated for all groups, and whereas a curative effect of Ferula elaeochytris could be seen, its protective effect was higher than its curative effect.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140118691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nobiletin alleviates methotrexate-induced hepatorenal toxicity in rats. Nobiletin 可减轻甲氨蝶呤引起的大鼠肝肾毒性。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-04-02 DOI: 10.1080/10520295.2024.2335168

Filiz Kazak, Ahmet Uyar, Pinar Coskun, Turan Yaman

{"title":"Nobiletin alleviates methotrexate-induced hepatorenal toxicity in rats.","authors":"Filiz Kazak, Ahmet Uyar, Pinar Coskun, Turan Yaman","doi":"10.1080/10520295.2024.2335168","DOIUrl":"10.1080/10520295.2024.2335168","url":null,"abstract":"We investigated the possible ameliorative effects of nobiletin (NBL) against methotrexate (MTX)-induced hepatorenal toxicity in rats. Twenty-eight Wistar albino rats were randomly divided into four groups, namely: Control; MTX (administered 20 mg/kg MTX); MTX+NBL (administered 20 mg/kg MTX and 10 mg/kg NBL per day); and NBL (administered 10 mg/kg/day NBL). Histopathological, immunohistochemical and biochemical analyses were performed on the kidney and liver tissues of rats at the end of the study. MTX caused renal toxicity, as indicated by increases in malondialdehyde (MDA) and caspase-3, as well as decreases in reduced glutathione (GSH), glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx), catalase (CAT) and B-cell lymphoma-2 (Bcl-2). MTX also caused hepatotoxicity, as indicated by increases in 8-hydroxy-2'-deoxyguanosine (8-OHdG), tumor necrosis factor alpha (TNF-α), MDA and caspase-3 and decrease in interleukin 10 (IL-10), GSH, total antioxidant capacity, GPx, G6PD, CAT and Bcl-2. MTX caused histopathological changes in kidney and liver tissues indicating tissue and cellular damage. Administration of NBL concurrently with methotrexate reduced oxidative stress, inflammatory and apoptotic signs, and prevented kidney and liver damage caused by methotrexate. We consider NBL has attenuating and ameliorating effects on methotrexate-induced hepatorenal toxicity.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140334560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative effects of Myrtus communis L. extract involving the inhibition of oxidative stress on high fat diet-induced testis damage in rats. 香桃木提取物对高脂饮食诱发的大鼠睾丸损伤具有抑制氧化应激的改善作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-04-29 DOI: 10.1080/10520295.2024.2344491

Busra Coskunlu, M Kutay Koroglu, Irem Hersek, Busra Ertas, Ali Sen, Goksel Sener, Feriha Ercan

{"title":"Ameliorative effects of Myrtus communis L. extract involving the inhibition of oxidative stress on high fat diet-induced testis damage in rats.","authors":"Busra Coskunlu, M Kutay Koroglu, Irem Hersek, Busra Ertas, Ali Sen, Goksel Sener, Feriha Ercan","doi":"10.1080/10520295.2024.2344491","DOIUrl":"10.1080/10520295.2024.2344491","url":null,"abstract":"The possible protective effects of Myrtus communis L. (MC) extract on a high fat diet (HFD)-induced testicular injury in a rat model were investigated using histological and biochemical methods. Wistar albino rats were divided into three groups: a standard diet control group; a HFD group; and an HFD+MC group. The HFD and HFD+MC groups were fed with a HFD for 16 weeks. MC extract (100 mg/kg) was given orally five days a week to the rats in the HFD+MC group during the last four weeks of the experiment. Leptin, triglyceride, high-density lipoproteins, cholesterol, estrogen, testosterone, LH and FSH were analyzed in blood serum. Sperm parameters were evaluated from the epididymis. Testicular morphology, proliferative, apoptotic and NADPH oxidase-2 (NOX2)-positive cells were evaluated histologically. Testicular oxidative stress parameters were analyzed biochemically. In the HFD group, lipid and hormone profiles were changed, abnormal spermatozoa, degenerated seminiferous tubules with apoptotic and NOX2-positive cells were increased in number, and sperm motility and germinal proliferative cells decreased compared to the control group. Moreover, testicular malondialdehyde, 8-hydroxy-2-deoxyguanosine and myeloperoxidase levels increased, whereas glutathione and superoxide dismutase levels decreased in the HFD group compared to the control group. All these histological and biochemical features were ameliorated by MC treatment of HFD-fed rats. In conclusion, HFD caused alterations in sperm parameters and testicular morphology by increasing oxidative damage and apoptosis. MC extract may have potential protective effects by inhibiting oxidative damage.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140861838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-04-05 DOI: 10.1080/10520295.2024.2335836

引用次数: 0

Stains recently certified. 污渍最近通过了认证。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-03-22 DOI: 10.1080/10520295.2024.2331320

引用次数: 0

Reviewers for Biotechnic & Histochemistry, 2023 2023 年生物技术与组织化学》审稿人

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-03-06 DOI: 10.1080/10520295.2024.2303898

引用次数: 0

β-Glucan ameliorates cisplatin-induced oxidative and histological damage in kidney and liver of rats. β-葡聚糖可改善顺铂诱导的大鼠肝肾氧化损伤和组织损伤

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-02-01 Epub Date: 2024-03-06 DOI: 10.1080/10520295.2024.2320626

Kürşat Kaya, O Ciftci, N Basak Turkmen, A Taşlıdere, C C Gül

{"title":"β-Glucan ameliorates cisplatin-induced oxidative and histological damage in kidney and liver of rats.","authors":"Kürşat Kaya, O Ciftci, N Basak Turkmen, A Taşlıdere, C C Gül","doi":"10.1080/10520295.2024.2320626","DOIUrl":"10.1080/10520295.2024.2320626","url":null,"abstract":"We investigated the effects of β-glucan (βg) on kidney and liver damage caused by cisplatin (CP), an antineoplastic agent widely used to treat many types of cancer, in a rat model. The side effects of CP in many tissues and organs limit its usage. βg is a natural polysaccharide that is an effective free radical scavenger. A total of 28 rats were randomly divided into four groups. Group 1 was a non-intervention control, only feed and water were given. Group 2 was administered 7 mg/kg CP in a single dose. Group 3 was administered 50 mg/kg βg orally for 14 days. Group 4 was administered βg for 14 days, following a single dose of CP. At the end of the experiment, kidney and liver tissues were evaluated biochemically and histopathologically. Increased thiobarbituric acid-reactive substances (TBARS) levels, as well as decreased catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and reduced glutathione (GSH) levels, as well as histological damage, were noted in both the kidney and liver tissues of the CP group. However, βg treatment prevented the oxidative and histopathological effects of CP. The study demonstrates the protective efficacy of βg against CP-induced kidney and liver damage through the effect of its antioxidant properties.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140038637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebral cavernous malformation proteins, CCM1, CCM2 and CCM3, are decreased in metastatic lesions in a murine breast carcinoma model. 小鼠乳腺癌模型转移灶中的脑海绵畸形蛋白（CCM1、CCM2 和 CCM3）减少。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-02-01 Epub Date: 2024-01-31 DOI: 10.1080/10520295.2024.2305114

Mansur Cici, Sayra Dilmac, Gunes Aytac, Gamze Tanriover

{"title":"Cerebral cavernous malformation proteins, CCM1, CCM2 and CCM3, are decreased in metastatic lesions in a murine breast carcinoma model.","authors":"Mansur Cici, Sayra Dilmac, Gunes Aytac, Gamze Tanriover","doi":"10.1080/10520295.2024.2305114","DOIUrl":"10.1080/10520295.2024.2305114","url":null,"abstract":"Three genes are associated with cerebral cavernous malformations (CCMs): CCM1, CCM2 and CCM3. These genes participate in microvascular angiogenesis, cell-to-cell junctions, migration and apoptosis. We evaluated the expression in vivo of CCM genes in primary tumors and metastastases in a murine model of metastatic breast carcinoma. We used cell lines obtained from metastasis of 4T1, 4TLM and 4THM breast cancer to liver and heart. These cells were injected into the mammary ridge of Balb/C female mice. After 27 days, the primary tumors, liver and lung were removed and CCM proteins were assessed using immunohistochemistry and western blot analysis. CCM proteins were expressed in primary tumor tissues of all tumor-injected animals; however, no CCM protein was expressed in metastatic tumor cells that migrated into other tissues. CCM proteins still were observed in the lung and liver tissue cells. Our findings suggest that CCM proteins are present during primary tumor formation, but when these cells develop metastatic potential, they lose CCM protein expression. CCM protein expression was lost or reduced in metastatic tissues compared to the primary tumor, which indicates that CCM proteins might participate in tumorigenesis and metastasis.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139641562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0