Biotechnic & Histochemistry最新文献_第5页

Effects of an energy drink on myonectin in the liver, kidney and skeletal muscle of exercised rats. 能量饮料对运动大鼠肝脏、肾脏和骨骼肌中肌连蛋白的影响

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-02-01 Epub Date: 2024-01-31 DOI: 10.1080/10520295.2024.2305113

Emsal Cagla Avcu, Vedat Çınar, Yavuz Yasul, Taner Akbulut, Zarife Pancar, I Sa Aydemir, Suna Aydin, Mehmet Hanifi Yalcin, Suleyman Aydin

{"title":"Effects of an energy drink on myonectin in the liver, kidney and skeletal muscle of exercised rats.","authors":"Emsal Cagla Avcu, Vedat Çınar, Yavuz Yasul, Taner Akbulut, Zarife Pancar, I Sa Aydemir, Suna Aydin, Mehmet Hanifi Yalcin, Suleyman Aydin","doi":"10.1080/10520295.2024.2305113","DOIUrl":"10.1080/10520295.2024.2305113","url":null,"abstract":"Myonectin is a hormone that is produced mainly by skeletal muscle. We investigated the effects of exercise and energy drink (ED) administration on myonectin expression in skeletal muscle, liver and kidney tissue in rats; myonectin is produced by all three tissues. We used 28 male albino rats in four groups: untreated control (C), exercise (E), energy drink (ED) and exercise + energy drink (E + ED). The E and E + ED groups were exercised using a treadmill for 4 weeks. We also administered 3.5 ml/kg/day ED during week 1, 7 ml/kg/day during week 2 and 10 ml/kg/day during weeks 3 and 4 in the E and E + ED groups. We used ELISA to measure the levels of myonectin in skeletal muscle, liver and kidney tissues. We used immunohistochemical staining to investigate the localization and intensity of myonectin in these tissues. The amount of myonectin in skeletal muscle tissue was increased significantly in all experimental groups compared to group C. The amount of myonectin in the ED group was significantly greater than group E. No significant difference was observed in liver tissue; however, the amount of myonectin in the liver of group C was the greatest among all groups. The amount of myonectin in kidney tissue exhibited no significant difference among groups. Consumption of ED during exercise increased the amount of myonectin in kidney and skeletal muscle tissues and decreased it in liver tissue. We suggest that consumption of ED might adapt metabolism to incresed exercise by controling synthesis of myonectin in liver, kidney and skeletal muscle.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139641563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of asprosin and meteorin-like peptide in progression of actinic keratosis to squamous cell carcinoma. 天冬氨酸和陨石样肽在光化性角化病向鳞状细胞癌发展过程中的作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-02-01 Epub Date: 2024-01-09 DOI: 10.1080/10520295.2024.2302016

Esma Inan Yuksel, Demet Cicek, Betul Demir, Nevin Kocaman, Ilknur Calik, Tuncay Kuloglu

{"title":"Role of asprosin and meteorin-like peptide in progression of actinic keratosis to squamous cell carcinoma.","authors":"Esma Inan Yuksel, Demet Cicek, Betul Demir, Nevin Kocaman, Ilknur Calik, Tuncay Kuloglu","doi":"10.1080/10520295.2024.2302016","DOIUrl":"10.1080/10520295.2024.2302016","url":null,"abstract":"Squamous cell carcinoma (SCC) often develops from an underlying premalignant lesion. Factors that affect the progression of actinic keratosis (AK) to invasive SCC are not fully known. Asprosin (ASP) and meteorin-like peptide (METRNL) are adipokines that are involved primarily in glucose metabolism. We investigated the expression of ASP and METRNL in AK and SCC to evaluate the role of these adipokines in the development of SCC. We used 15 SCC specimens, 12 AK specimens and 12 healthy control skin specimens. ASP and METRNL protein expression in tumor and surrounding tissue was evaluated using immunohistochemistry. ASP expression in tumor tissue was significantly greater in the SCC group than in the control and AK groups, but it did not differ significantly between the AK and control groups. A positive correlation was observed for both ASP and METRNL expressions between tumor tissue and adjacent epidermis, hair follicles, sebaceous gland, eccrine gland, inflammatory cells and vascular structures. ASP and METRNL may exert pro-tumor effects toward development of invasive SCC. The expression intensity of ASP and METRNL can be used as a biomarker of risk of progression to SCC.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relation of Langerhans cell size to buccal carcinoma. 朗格汉斯细胞大小与口腔癌的关系

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2024-02-01 Epub Date: 2024-01-31 DOI: 10.1080/10520295.2024.2305499

John Kandam Kulathu Mathew, Pranay Gaikwad, Rajadoss M K Pandian, Grace Rebekah, Suganthy Rabi

{"title":"Relation of Langerhans cell size to buccal carcinoma.","authors":"John Kandam Kulathu Mathew, Pranay Gaikwad, Rajadoss M K Pandian, Grace Rebekah, Suganthy Rabi","doi":"10.1080/10520295.2024.2305499","DOIUrl":"10.1080/10520295.2024.2305499","url":null,"abstract":"Oral cancer decreases quality of life despite timely medical management. The carcinogens in tobacco products and their role in tumorigenesis are well documented. Langerhans cells (LCs) are a subset of antigen-presenting cells (APCs) that monitor the tumor microenvironment and engulf carcinogens and foreign bodies. We investigated the distribution and size of LCs and their relation to the mode of tobacco consumption and clinical outcome in patients with buccal carcinoma. We recruited patients with oral cancer who were scheduled for tumor excision and men with urethral stricture undergoing substitution urethroplasty using buccal mucosa. Normal and tumor-adjacent tissues were stained with CD1a antibody. The distribution and mean diameter of 100 LCs/patient were determined. We found significantly smaller LCs in patients who chewed only tobacco compared to those who consumed tobacco by other means. The size of LCs decreased significantly with progressive stages of malignant disease. We found that patients with larger LCs survived longer than those with smaller LCs during an average follow-up of 24 months. We suggest a relation between the size of LCs and clinical outcomes in patients with buccal carcinoma.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139641564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of ABCB5 immunostained epithelial stem cells in oral squamous cell carcinoma, inflammatory gingival hyperplasia and normal mucosa 评估口腔鳞状细胞癌、炎性牙龈增生和正常粘膜中的 ABCB5 免疫染色上皮干细胞

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-12-11 DOI: 10.1080/10520295.2023.2292062

M. G. Aadithi, Bose Divya, G. Nandhini, Krishnan Rajkumar, A. Ramesh Kumar, R. Sarangarajan

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-27 DOI: 10.1080/10520295.2024.2286747

引用次数: 0

Use of hyaluronic acid matrix in dorsal augmentation rhinoplasty. 透明质酸基质在隆鼻术中的应用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-08-30 DOI: 10.1080/10520295.2023.2248889

Zuleyha Erisgin, Omer Hizli, Guven Yildirim, Cengiz Sivrikaya, Ahmet Burcin Sarisoy, Yonca Avci, Kursat Murat Ozcan

{"title":"Use of hyaluronic acid matrix in dorsal augmentation rhinoplasty.","authors":"Zuleyha Erisgin, Omer Hizli, Guven Yildirim, Cengiz Sivrikaya, Ahmet Burcin Sarisoy, Yonca Avci, Kursat Murat Ozcan","doi":"10.1080/10520295.2023.2248889","DOIUrl":"10.1080/10520295.2023.2248889","url":null,"abstract":"Augmentation rhinoplasty sometimes is required for patients with saddle nose deformity caused by failed rhinoplasty or facial trauma; finding appropriate grafting material remains a significant problem for this procedure. We investigated hyaluronic acid matrix as an allograft for dorsal augmentation rhinoplasty in a rabbit model. We performed an osteotomy on the nasal bones of eight rabbits. Four animals were sham operated as the control group and four were administered a mixture of saline-gelled hyaluronic acid matrix and sliced cartilage. Ultrasonography and three-dimensional reconstruction tomography were performed at the end of the experimental period. After sacrifice of the animals, nasal tissues were examined for histopathology, and both collagen scores and number of capillaries were compared between the two groups. Increased collagen and capillaries were apparent in the hyaluronic acid matrix group compared to controls. The median collagen score was significantly greater for the hyaluronic acid matrix group than for the control group. Although the number of capillaries for the hyaluronic acid matrix group was greater than for the control group, the difference was not statistically significant. Three weeks is sufficient for adhesion of ends of fractures in clinical practice; however, we found no ossification at this time in either group. A hyaluronic acid matrix may be a useful alternative supplement for dorsal augmentation rhinoplasty. Development of collagen was commensurate with membranous ossification; however, assessment of complete ossification requires a longer experimental period.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10112984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic apoptotic effects of ethanolic extracts of ginger and Ganoderma lucidum in a colorectal cancer cell line. 生姜和灵芝乙醇提取物对结直肠癌细胞凋亡的协同作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-03-27 DOI: 10.1080/10520295.2023.2190620

Amir Mohammad Saeedifar, Ali Ghazavi, Ghasem Mosayebi, Ali Ganji

{"title":"Synergistic apoptotic effects of ethanolic extracts of ginger and Ganoderma lucidum in a colorectal cancer cell line.","authors":"Amir Mohammad Saeedifar, Ali Ghazavi, Ghasem Mosayebi, Ali Ganji","doi":"10.1080/10520295.2023.2190620","DOIUrl":"10.1080/10520295.2023.2190620","url":null,"abstract":"Current conventional therapy for colorectal cancer includes surgery, radiation and chemotherapy, all of which produce side effects. Herbal medicine can control the side effects of conventional treatments. We investigated the synergistic effect of a mixture of Zingiber officinale Roscoe (Ginger) and Ganoderma lucidum extracts on colorectal cancer cell apoptosis in vitro. We prepared ethanolic extracts of ginger (GEE) and G. lucidum (GLEE). Cytotoxicity was evaluated using MTT assay and the half-maximal inhibitory concentration (IC50) of each extract was calculated. The effect of these extracts on apoptosis in cancer cells was assessed using flow cytometry; Bax, Bcl2 and caspase-3 gene expression was evaluated using real-time PCR. GEE and GLEE decreased CT-26 cell viability significantly in a dose-dependent manner; however, the combined application of GEE + GLEE was most effective. Bax:Bcl-2 gene expression ratio, caspase-3 gene expression and the number of apoptotic cells were increased significantly in CT-26 cells treated at the IC50 level of each compound, especially in the GEE + GLEE treatment group. Combined ginger and Ganoderma lucidum extracts exhibited synergistic antiproliferative and apoptotic effects on colorectal cancer cells.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10038622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Influence of type 2 diabetes on immunohistochemical detection of TRAF6, cFos and NFATC1 in the gingiva in cases of chronic periodontitis. 2型糖尿病对慢性牙周炎患者牙龈TRAF6、cFos和NFATC1免疫组织化学检测的影响。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-07-24 DOI: 10.1080/10520295.2023.2236543

R Mathangi, Vidyarani Shyamsundar, A Meenakshi, N Aravindha Babu, Yaongamphi Vashum, S Shila

引用次数: 0

Classification and naming of polymethine dyes used as staining agents for microscopy. A short guide for biomedical investigators. 显微镜染色用聚甲基染料的分类和命名。给生物医学研究者的简短指南。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10520295.2023.2263837

Heinz Mustroph, Richard W Horobin

{"title":"Classification and naming of polymethine dyes used as staining agents for microscopy. A short guide for biomedical investigators.","authors":"Heinz Mustroph, Richard W Horobin","doi":"10.1080/10520295.2023.2263837","DOIUrl":"10.1080/10520295.2023.2263837","url":null,"abstract":"The scientific literature contains many accounts of application of polymethine dyes, including cyanine dyes, as imaging agents, i.e., \"biological stains,\" for microscopic investigation of biological materials. Currently, many such dyes are used as probes for living cells, i.e., \"fluorescent probes.\" Polymethine dyes are defined here by two criteria. First, they possess a conjugated chain of (2n + 1) sp2-hybridized carbon atoms that connect a terminal π-electron-accepting (π-electron withdrawing) group with a terminal π-electron-donating group. Second, they have an odd number (2n + 3) of π-centers and an even number (2n + 4) of π-electrons in this chain, where n equals the number of -CR2=CR3- groups, usually vinylene groups -CH=CH-. Commercialization of diverse chemical types of many polymethine dyes has been attempted. The dyes that have achieved wide application, however, are limited in number and it is these dyes that are emphasized here. Because these polymethine dyes sometimes have been described by confusing, and sometimes confused, names, we clarify here the chemical categories and names of such dyes for the nonchemist, biomedical end user of such imaging agents. Nevertheless, the nomenclature presented here is not intended to replace the traditional \"chromophore\" categories of dyestuff chemistry, because the latter are held in place both by wide usage and by venerable authorities, such as the Colour Index.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50156959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of canagliflozin in ameliorating isoprenaline induced cardiomyocyte oxidative stress via the heme oxygenase-1 mediated pathway. 卡格列净通过血红素加氧酶-1介导的途径改善异丙肾上腺素诱导的心肌细胞氧化应激的作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10520295.2023.2262390

Ahmed Ahmed, Dina Abdel-Rahman, Ehab M Hantash

引用次数: 0