Effects of vitamin D and common nettle (Urtica dioica) extract administration on Mn-SOD and Catalase (CAT) secretion in the colon tissues of rats with experimentally induced Crohn's disease.

Abstract

We have examined some effects of administering vitamin D and extract of common nettle (Urtica dioica) to rats with experimentally induced Crohn's disease (CR). Body weight and colon length were lower in the CR group than in normal controls, whereas scores for histopathologic changes seen in sections stained by the H&E and PAS methods were lower in rats with CR than in those that also received either vitamin D (CRD) or nettle extract (CRI). Strong manganese-superoxide dismutase (Mn-SOD) immunoreactivity was detected in the crypt epithelium of the CR and CRI groups and in the lymphoid tissue of the CRD group. Weak catalase (CAT) immunoreactivity in the crypt epithelium in the CR, CRI, and CRD groups and strong CAT immunoreactivity in the lymphoid tissue in the CR group were also observed. Our results reveal that administering either vitamin D and common nettle extract can have augment Mn-SOD and CAT expression in colon tissues and contribute to alleviation of some complications of experimental Crohn's disease.