Biotechnic & Histochemistry最新文献_第10页

Protective effect of adenosine triphosphate and benidipine separately or together against cardiotoxicity caused by bevacizumab. 三磷酸腺苷和苯地平单独或联合对贝伐单抗引起的心脏毒性的保护作用。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2153385

Erkan Yıldırım, Nilgun Yıldırım, Mahir Cengiz, Gulce Naz Yazıcı, Resit Coskun, Bahadır Suleyman, Abdulkadir Coban, Halis Suleyman

{"title":"Protective effect of adenosine triphosphate and benidipine separately or together against cardiotoxicity caused by bevacizumab.","authors":"Erkan Yıldırım, Nilgun Yıldırım, Mahir Cengiz, Gulce Naz Yazıcı, Resit Coskun, Bahadır Suleyman, Abdulkadir Coban, Halis Suleyman","doi":"10.1080/10520295.2022.2153385","DOIUrl":"https://doi.org/10.1080/10520295.2022.2153385","url":null,"abstract":"Bevacizumab is a recombinant humanized monoclonal antibody whose adverse effects include cardiotoxicity. We investigated whether using adenosine triphosphate (ATP) or benidipine either separately or together protects against cardiac damage induced by bevacizumab in rats. Forty Wistar albino male rats were allocated to five groups of eight: bevacizumab (Bv), ATP + bevacizumab (ABv), benidipine + bevacizumab (BBv), ATP + benidipine + bevacizumab (ABBv) and untreated controls. Rats in the ABv group were injected intraperitoneally (i.p.) with 2 mg/kg ATP. The BBv group was given 4 mg/kg benidipine by oral gavage. The ABBv group was injected i.p. with 2 mg/kg ATP and simultaneously administered 4 mg/kg benidipine orally. One hour after administration of ATP, benidipine or normal saline, the Bv, ABv, BBv and ABBv groups were injected i.p. with 10 mg/kg bevacizumab. Malondialdehyde (MDA) and total glutathione (tGSH) levels were measured in cardiac tissue, and troponin I (TP I) and creatine kinase MB (CK-MB) levels were measured in blood samples. Tissue samples were examined for histopathology. We found the lowest TP I, CK-MB and MDA levels and the highest tGSH level in the ABBv group; these results were similar to the control group. Nuclei of cardiomyocytes in the BV group were misshapen and shrunken, and myofibers were disrupted; we also observed eosinophilic degeneration and interstitial edema. Blood capillaries were dilated and congested. We observed amelioration of these findings in the ABBv group. We found that ATP and benidipine alone or in combination reduced cardiac damage associated with the use of bevacizumab. ATP + benidipine combined therapy produced the most favorable results.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 3","pages":"193-200"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualization of the carotid body in situ in fixed human carotid bifurcations using a xylene-based tissue clearing method. 使用二甲苯为基础的组织清除方法原位观察固定人颈动脉分叉的颈动脉体。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2022.2140831

Daphne Verdoorn, Cindy Gj Cleypool, Claire Mackaaij, Ronald Law Bleys

引用次数: 0

Effects of diazoxide on streptozotocin induced β cell damage via HSP70/HSP90/TLR4/AMPK signaling pathways. 二氮氧化物通过HSP70/HSP90/TLR4/AMPK信号通路对链脲佐菌素诱导的β细胞损伤的影响

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-04-01 DOI: 10.1080/10520295.2023.2168757

Salih Tunc Kaya

{"title":"Effects of diazoxide on streptozotocin induced β cell damage via HSP70/HSP90/TLR4/AMPK signaling pathways.","authors":"Salih Tunc Kaya","doi":"10.1080/10520295.2023.2168757","DOIUrl":"https://doi.org/10.1080/10520295.2023.2168757","url":null,"abstract":"I investigated the effects of diazoxide, a mitochondrial potassium channel opener, on streptozotocin (STZ) induced pancreatic β cell damage via the HSP70/HSP90/TLR4/AMPK signaling pathways in vitro. I used the pancreatic β cell line, 1.1B4, to create four groups: control, STZ treated, diazoxide treated, STZ + diazoxide treated. The STZ treated cells were exposed to 20 µM STZ for 2 h with or without 100 µM diazoxide for 24 h. Total antioxidant status (TAS), total oxidant status (TOS), cell viability and mitochondrial membrane potential (MMP) were measured. Expression of ATP-sensitive potassium channel (KATP) subunits, heat shock protein-70 (HSP70), heat shock protein-90 (HSP90), toll-like receptor 4 (TLR4), AMP-activated protein kinase (AMPK) and some apoptotic proteins were detected using western blotting. Apoptosis was assessed using TUNEL staining. STZ increased TOS and OSI in the pancreatic β cells; however, diazoxide failed to improve oxidative stress. Also, STZ increased tunnel positive cells in the pancreatic β cells. Diazoxide decreased the tunnel positive cells in the STZ treated β cell. STZ decreased MMP; however, diazoxide did not normalize MMP in the STZ induced β cells. Diazoxide increased the HSP70:HSP90 protein expression ratio. STZ decreased expression of AMPK and subunits of KATP channel and increased the expression of caspase-3 and TLR4 protein; diazoxide normalized the expression of all proteins studied. KATP channel opening by diazoxide protects pancreatic β cells against STZ toxicity via HSP70/HSP90/TLR4/AMPK signaling.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 3","pages":"210-219"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9151093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-03-01 DOI: 10.1080/10520295.2023.2184029

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-02-08 DOI: 10.1080/10520295.2023.2174670

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-02-07 DOI: 10.1080/10520295.2023.2174669

引用次数: 0

Stains recently certified. 最近认证的污渍。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-02-07 DOI: 10.1080/10520295.2023.2174668

引用次数: 1

Expression of nuclear factor-erythroid 2-related factor 2 (Nrf2) in mouse uterus during the peri-implantation period. 核因子-红细胞2相关因子2 (Nrf2)在围着床期小鼠子宫中的表达。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-02-01 DOI: 10.1080/10520295.2022.2127156

Hakan Soylu, Kubra Aksu, Ezgi Golal, Ismail Ustunel, V Nimet Izgut-Uysal, Nuray Acar

引用次数: 0

Differential gene expression of ADAMTS-1, ADAMTS-9 and TIMP-3 in periodontitis. 牙周炎中ADAMTS-1、ADAMTS-9和TIMP-3基因的差异表达。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-02-01 DOI: 10.1080/10520295.2022.2121857

M Ayşe Tayman, İsmail Koyuncu

{"title":"Differential gene expression of ADAMTS-1, ADAMTS-9 and TIMP-3 in periodontitis.","authors":"M Ayşe Tayman, İsmail Koyuncu","doi":"10.1080/10520295.2022.2121857","DOIUrl":"https://doi.org/10.1080/10520295.2022.2121857","url":null,"abstract":"A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) are metalloproteinases that bind to components of the extracellular matrix (ECM) to regulate tissue remodeling and homeostasis. ADAMTS can be inhibited by tissue inhibitors of metalloproteinases (TIMPs). Expression of ADAMTS increases under inflammatory conditions. We investigated the mRNA expression of ADAMTS-1, ADAMTS-9 and TIMP-3 genes in both healthy gingival tissues and periodontitis. Clinical periodontal measurements were conducted and gingival biopsies were obtained from stage IIIgrade C generalized periodontitis and healthy (control) groups. mRNA expression was evaluated using real-time quantitative polymerase chain reaction (RTqPCR). All clinical periodontal parameters were significantly higher in the periodontitis group than for the control group. ADAMTS-1 levels were significantly higher in the periodontitis group and were significantly correlated with clinical attachment level and probing pocket depth. Differences in ADAMTS-9 and TIMP-3 mRNA in the periodontitis group compared to the control group were not statistically significant. Increased ADAMTS-1 mRNA expression in periodontitis indicates that members of the ADAMTS family of metalloproteinases are associated with pathogenesis and progression of periodontal disease. Maintaining balance between ADAMTS and TIMP is important for limiting ECM catabolism and preventing tissue damage.","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"98 2","pages":"126-131"},"PeriodicalIF":1.6,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10656264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Use of asprosin and subfatin for differential diagnosis of serous ovarian tumors. 应用亚脂素和亚脂素鉴别诊断浆液性卵巢肿瘤。

IF 1.6 4区生物学

Biotechnic & Histochemistry Pub Date : 2023-02-01 DOI: 10.1080/10520295.2022.2135763

Miyase Mirzaoglu, Seyda Yavuzkir, Cetin Mirzaoglu, Nurdan Yurt, Adile Ferda Dagli, Sena Ozcan Yildirim, İbrahim Sahin, Suleyman Aydin

引用次数: 3