Protective effect of Arum maculatum against dextran sulfate sodium induced colitis in rats.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-07-03 DOI:10.1080/10520295.2023.2225226

Gülsüm Toparlı Doğan, Remziye Aysun Kepekçi, Nuray Bostancıeri, Mehmet Tarakçıoğlu

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引用次数: 0

Abstract

Ulcerative colitis (UC) is an inflammatory disease of the large intestine that is characterized by diarrhea, bloody stools, abdominal pain and mucosal ulceration. UC is treated with nonsteroidal anti-inflammatory drugs, corticosteroids or immunosuppressants, but long-term use of these drugs can cause adverse effects. Arum maculatum is used as a traditional treatment for digestive system disorders, but its use for treatment of UC has not been investigated rigorously. We investigated the possible protective effect of a methanol extract of A. maculatum against dextran sulfate sodium (DSS) induced experimental UC in rats. Total phenolic and flavonoid contents of the extract were 32.919 ± 1.125 mg gallic acid equivalent (GAE)/g and 52.045 ± 7.902 µg rutin equivalent (RE)/mg, respectively. The half-maximal inhibitory concentration (IC₅₀) for the extract was 105.76 µg/ml according to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay. Effects of A. maculatum extract on UC induced by DSS were assessed both macroscopically and histologically. We also investigated effects of A. maculatum extract on malondialdehyde (MDA) levels and the oxidative stress index (OSI) in normal rats and rats with UC. We found that treatment with A. maculatum extract protected the colon against DSS induced UC in a dose-dependent manner.

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黄对右旋糖酐硫酸钠诱导的大鼠结肠炎的保护作用。

溃疡性结肠炎（UC）是一种以腹泻、便血、腹痛和粘膜溃疡为特征的大肠炎症性疾病。UC是用非甾体抗炎药、皮质类固醇或免疫抑制剂治疗的，但长期使用这些药物会引起不良反应。黄斑阿鲁姆被用作消化系统疾病的传统治疗方法，但其用于UC的治疗尚未得到严格研究。我们研究了斑蝥甲醇提取物对右旋糖酐硫酸钠（DSS）诱导的大鼠实验性UC的可能保护作用。提取物的总酚类和类黄酮含量分别为32.919±1.125 mg没食子酸当量（GAE）/g和52.045±7.902µg芦丁当量（RE）/mg。根据1,1-二苯基-2-苦基肼（DPPH）自由基清除活性测定，提取物的半最大抑制浓度（IC50）为105.76µg/ml。从宏观和组织学两方面评估了黄斑A.maculatum提取物对DSS诱导的UC的影响。我们还研究了斑蝥提取物对正常大鼠和UC大鼠丙二醛（MDA）水平和氧化应激指数（OSI）的影响。我们发现，黄斑A.maculatum提取物以剂量依赖的方式保护结肠免受DSS诱导的UC的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.