使用烷烃提取物(Alkanna tinctoria)作为组织学红色脂质染色和蓝色复染的来源。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnic & Histochemistry Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI:10.1080/10520295.2023.2271397
Hayfaa A Alshamar, Richard W Dapson
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引用次数: 0

摘要

Alkanet(Alkanna tinctoria)是一种原产于欧洲、亚洲和中东部分地区的植物。数千年来,它一直被用作药物和纺织品、食品和化妆品的着色剂。这种植物根部的提取物已与媒染剂一起用于染色细胞核。我们在这里描述了这种植物的不同提取物的多功能性,可以将脂质染成红色,并将某些其他组织元素复染成蓝色。颜色变化和选择性差异染色是由于溶剂极性和pH。提取物含有许多化学物质,所有这些都是指示染料萘天青的衍生物。我们的红色提取物在pH 7以下是非离子的,并从其100%异丙醇的极性溶剂中分离出非极性脂质。蓝色提取物在70%异丙醇中的高pH下是双离子的,并与阳离子组织结构如胶原、肌肉和其他细胞的细胞质离子结合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using extract from alkanet (Alkanna tinctoria) as a source of both a red lipid stain and a blue counterstain for histology.

Alkanet (Alkanna tinctoria) is a plant native to and cultivated in parts of Europe, Asia and the Middle East. It has been used for thousands of years as a medicinal agent and as a colorant for textiles, food and cosmetics. An extract from the root of this plant has been used with a mordant to stain nuclei. We describe here the versatility of different extracts from this plant to stain lipids red and to counterstain certain other tissue elements blue. The color variation and selective differential staining is due to solvent polarity and pH. Extracts contain numerous chemical species, all of which are derivatives of the indicator dye, naphthazurin. Our red extract is nonionic below pH 7 and partitions from its somewhat polar solvent of 100% isopropanol to nonpolar lipids. The blue extract is dianionic at high pH in 70% isopropanol and binds ionically to cationic tissue structures such as collagen, muscle and cytoplasm of other cells.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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