罗曼诺夫斯基染色:从化学角度看历史、最新进展和未来前景。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI:10.1080/10520295.2023.2273860
Valeriy Kalinin, Pavel Padnya, Ivan Stoikov
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引用次数: 0

摘要

罗曼诺夫斯基染色是19世纪末和20世纪初诊断血液学和细胞病理学的一项重要方法突破;几十年来,它促进了生物学家、血液学家和病理学家研究血细胞的工作。尽管对罗曼诺夫斯基染色进行了一个多世纪的研究,但尚未发表系统综述来解释产生“罗曼诺夫斯基效应”或“罗曼诺斯基-吉姆萨效应”(RGE)的化学过程,即蓝色染料与曙红相互作用产生的紫色,而不仅仅是由于它们同时存在。我们的综述试图在化学家和生物医学科学家之间架起一座桥梁,并总结亚甲基蓝(MB)脱甲基的可用数据,以及通过光、酶系统和微生物将MB还原和分解为更简单的化合物的相关数据。为了做到这一点,我们分析了在酸和碱存在下以及由于光的作用而歧化MB脱甲基机制的现代数据。我们还通过应用实验和计算的物理化学参数,包括染料DNA结合常数和这些配体分子中的电子密度分布,解释了为什么只有当天青B或在较小程度上存在天青a时才会发生RGE。最后,我们讨论了通过对先前已知的染料进行修饰来获得新品种的罗曼诺夫斯基染料的现代技术。我们希望我们的批判性文献研究将帮助科学家更好地了解用这种染料染色细胞的化学和物理化学过程和机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Romanowsky staining: history, recent advances and future prospects from a chemistry perspective.

Romanowsky staining was an important methodological breakthrough in diagnostic hematology and cytopathology during the late 19th and early 20th centuries; it has facilitated for decades the work of biologists, hematologists and pathologists working with blood cells. Despite more than a century of studying Romanowsky staining, no systematic review has been published that explains the chemical processes that produce the "Romanowsky effect" or "Romanowsky-Giemsa effect" (RGE), i.e., a purple coloration arising from the interaction of an azure dye with eosin and not due merely to their simultaneous presence. Our review is an attempt to build a bridge between chemists and biomedical scientists and to summarize the available data on methylene blue (MB) demethylation as well as the related reduction and decomposition of MB to simpler compounds by both light and enzyme systems and microorganisms. To do this, we analyze modern data on the mechanisms of MB demethylation both in the presence of acids and bases and by disproportionation due to the action of light. We also offer an explanation for why the RGE occurs only when azure B, or to a lesser extent, azure A is present by applying experimental and calculated physicochemical parameters including dye-DNA binding constants and electron density distributions in the molecules of these ligands. Finally, we discuss modern techniques for obtaining new varieties of Romanowsky dyes by modifying previously known ones. We hope that our critical literature study will help scientists understand better the chemical and physicochemical processes and mechanisms of cell staining with such dyes.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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